Solid bait containing insect growth regulator and its preparation method and application

Abstract

The present application relates to the field of pest control technology, and specifically discloses a solid bait containing an insect growth regulator, and its preparation method and application. The solid bait includes the following components by weight: 60-70 parts of wheat bran, 10-15 parts of maltose, 5-10 parts of oil, 0.1-3 parts of insect growth regulator, 5-15 parts of protein source, and 1-5 parts of preservative; its preparation method is: weigh maltose, oil, insect growth regulator, protein source, and preservative respectively, dissolve them with a volatile solvent, mix all components thoroughly, and after the volatile solvent is completely volatilized, add wheat bran and mix evenly to obtain the solid bait containing insect growth regulator. The solid bait of the present application can be used to control red fire ants. It is safe, low-toxic, environmentally friendly, and has no cross-resistance. It has good attraction and palatability, a slow lethality rate, is not prone to refusal to feed, and has a high control rate, providing a variety of options for the control of red fire ants.

Description

Technical Field

[0001] The present application relates to the technical field of insect pest control, and more specifically, to a solid bait containing an insect growth regulator and a preparation method and application thereof. Background Art

[0002] Red fire ants are originally distributed in South America. Many countries have listed them as major quarantine pests and strictly guard against them because of their high reproduction rate, strong vitality, ferocious habits, great harm, rapid spread, and difficulty in prevention and control. Once the ants enter a new area, due to the lack of natural enemies and other factors, the population will expand rapidly and erupt into a disaster in a short period of time, seriously affecting the safety of people and animals, agricultural and forestry production, ecological balance and economic development in the invaded areas.

[0003] One of the important means of preventing and controlling red fire ants in China is the poison bait trapping method, which mainly consists of food and pesticides for killing. Worker ants are used to forage and carry the poison bait back to the ant nest. Through feeding and poison transmission, other members are poisoned and die, thereby reducing the density of the red fire ant population and achieving a control effect.

[0004] At present, some bait pesticides used to control red fire ants, while playing a control role, their residual ingredients will pollute water, soil, etc., and produce different degrees of toxicity to non-target organisms such as fish, shrimp, and bees, which is very likely to cause damage to the ecological balance, and is not in line with the concept of green, environmental protection and sustainability; in addition, there are some fast-acting insecticides that can quickly kill some worker ants in a short period of time, but will lead to incomplete transmission of the poison. Among them, when faced with a large number of worker ants in the population, the unpoisoned ant colonies will generally abandon the old nest and build a new one. On the surface, the killing effect is very good, but in fact it cannot achieve the purpose of controlling red fire ants. In addition, the drug effect is fast, which is easy to produce a refusal to feed, which is not conducive to the transmission of active ingredients to the queen ants with reproductive functions, affecting the control effect. In addition, the number of products for the control of red fire ants in my country is small and relatively single, which is not suitable for the comprehensive control of red fire ants, nor is it conducive to delaying the resistance of red fire ants. Summary of the invention

[0005] In order to avoid damage to the environment caused by baits, improve the control effect of red fire ants, and delay the drug resistance of red fire ants, the present application provides a solid bait containing an insect growth regulator and a preparation method and application thereof.

[0006] In the first aspect, the present application provides a solid bait containing an insect growth regulator, adopting the following technical scheme: a solid bait containing an insect growth regulator, comprising the following components in parts by weight: 60-70 parts of wheat bran, 10-15 parts of maltose, 5-10 parts of oil, 0.1-3 parts of insect growth regulator, 5-15 parts of protein source, and 1-5 parts of preservative.

[0007] The solid bait is made of wheat bran, maltose, oil, insect growth regulator, protein source and preservative as raw materials. It has good attracting and killing effect on red fire ants, is safe, low-toxic, environmentally friendly, has no cross-resistance, has good attraction and palatability, has a slow lethality, is not prone to food refusal, has a high prevention and control rate, is easy to operate, etc., and has good application prospects.

[0008] The present application uses maltose as an auxiliary agent. As a kind of sugar substance, maltose can increase the feeding habit of red fire ants on bait and improve the control effect. Maltose has a certain viscosity. Mixing it into the solid bait can protect the effective ingredients from being easily affected by moisture and dilution, thereby ensuring the stability of the solid bait of the present application.

[0009] Optionally, the following components are included in parts by weight: 63.75-67 parts of wheat bran, 10-15 parts of maltose, 5-10 parts of oil, 0.25-2 parts of insect growth regulator, 10-15 parts of protein source, and 1-2.5 parts of preservative.

[0010] Optionally, the insect growth regulator is S-methoprene.

[0011] S-methoprene is an artificially synthesized chiral compound with biological activity. It has a similar structure and the same function as the natural insect juvenile hormone JH-III. It belongs to the class of biochemical pesticides called insect growth regulators (IGRs). It interferes with the normal growth process of target organisms, preventing larvae from successfully transforming into adults, thereby achieving the purpose of large-scale population control.

[0012] The molecular structure of S-methoprene is composed of C, H, and O elements. It is a low molecular weight trans-unsaturated fatty acid ester that decays rapidly under ultraviolet light. Its metabolite in animals and the environment is CO2, which leaves no residue. It is safe for mammals and non-target organisms and relatively safe for aquatic organisms. After being dragged into an ant nest, it will no longer have adverse effects on other non-target organisms and can be used in a variety of occasions.

[0013] The wheat bran used in the present application is the seed coat left after wheat is ground into flour. It is an easily available and common material. As the staple food in the bait, it is light and easy to carry, has a good luring effect on red fire ants, and is easy to make into a preparation with other ingredients. In addition, the wheat bran contains active polysaccharides and phenolic compounds. Combined with the loose and porous characteristics of the wheat bran, the combination of wheat bran and S-methoprene can slow down the degradation of S-methoprene and improve the stability of S-methoprene.

[0014] Optionally, the protein source is bone meal or insect meal.

[0015] Optionally, the oil is a combination of one or more of soybean oil, soybean oil, peanut oil, rapeseed oil, sesame oil, corn oil, cottonseed oil and wheat germ oil.

[0016] By adopting the above technical solution, the luring effect can be improved and the foraging time of red fire ants can be shortened.

[0017] Optionally, the preservative is a combination of one or more of sodium benzoate, potassium sorbate and propylparaben.

[0018] Since the solid bait of the present application contains a high proportion of processed grains, sugars, protein sources and other substances, it is easy to spoil and mildew in the natural state, thus causing the red fire ants to refuse to eat. By adding a preservative to the solid bait, one or a combination of sodium benzoate, potassium sorbate and propylparaben can be selected to effectively delay the mildew of the bait, ensure the bait's attractant effect, and prolong the action time of the present invention.

[0019] In a second aspect, the present application provides a method for preparing a solid bait containing an insect growth regulator, using the following technical solution:

[0020] The invention discloses a method for preparing a solid bait containing an insect growth regulator. The method comprises the following steps: respectively weighing maltose, oil, an insect growth regulator, a protein source and a preservative, dissolving them in a volatile solvent, and fully mixing all the components. After the volatile solvent is completely volatilized, wheat bran is added and mixed evenly to obtain the solid bait containing the insect growth regulator.

[0021] The present application prepares a solid bait by mixing S-methoprene with wheat bran and maltose in a certain proportion. The solid bait is a uniform loose substance with an oily aroma, has good attractant and palatability, can safely and efficiently kill red fire ant nests, has good ecological, environmental and application safety, and is a new type of environmentally friendly preparation.

[0022] Since wheat bran is loose and has a large specific surface area, it can be mixed with other components after the volatile solvent has evaporated, which can effectively disperse the other components and make the components in the solid bait more uniform.

[0023] Optionally, the volatile solvent comprises acetone, n-hexane or n-butanol.

[0024] The present application uses a volatile solvent to mix the ingredients other than wheat bran, so that the ingredients other than wheat bran can be mixed more evenly. Since the volatile components are completely volatilized after the ingredients other than wheat bran are mixed evenly, and will not remain in the final product, the present application does not limit the amount of the volatile solvent, and it is preferred that the ingredients other than wheat bran can be mixed evenly and volatilized as quickly as possible.

[0025] In a third aspect, the present application provides an application of a solid bait containing an insect growth regulator, using the following technical solution:

[0026] The application of a solid bait containing an insect growth regulator, wherein the solid bait is used to control red fire ants, and the solid bait is evenly placed within a radiation range of 80 to 120 cm from the center of the red fire ant nest.

[0027] The solid bait of the present application is evenly placed within a radiation range of 80 to 120 cm from the center of the red fire ant nest. The foraging worker ants bring the solid bait containing S-methoprene back to the ant nest to pass on the larvae through trophobia. S-methoprene works in the larvae to prevent the pupae from becoming worker ants and reproductive ants. When all the existing worker ants die, there are no new worker ants to replace them. Eventually, the ant colony dies due to hunger, achieving the purpose of destroying the entire ant nest. In addition, it can also prevent a large number of reproductive ants from migrating to build new nests.

[0028] Optionally, the solid bait is added in an amount of 10 to 30 g per ant nest.

[0029] By placing 10 to 30 g of solid bait within a radiation range of 80 to 120 cm from the center of each ant nest, more than 90% of the ant nest prevention and control effect can be achieved after 7 weeks of bait feeding.

[0030] In summary, this application has the following beneficial effects:

[0031] 1. The present application provides a solid bait containing an insect growth regulator, which can be used to control red fire ants. The solid bait is safe, low-toxic, environmentally friendly, has no cross-resistance, has good attractivity and palatability, has a slow lethality rate, is not prone to food refusal, has a high control rate, and provides a variety of options for the control of red fire ants.

[0032] 2. Compared with other fast-acting insecticides, since the effect of insect growth regulators is slower than that of traditional chemicals, the solid bait of this application will not kill red fire ants immediately. The number of ant colonies began to decrease from the third week and disappeared within 8 to 10 weeks. On the one hand, this application has no avoidance effect on foraging worker ants, and is not easy to cause food refusal, which is conducive to the transfer of active ingredients to the queen ant through feeding behavior, ensuring the ant killing effect; on the other hand, although the drug effect is slow, it can avoid ant colony migration and rebound, and can achieve efficient control of ant colonies. DETAILED DESCRIPTION

[0033] S-methoprene is an insect growth regulator. It does not kill larvae or adults directly with toxicity, but acts on larvae with juvenile hormones. Larvae die in large numbers in the pupal stage and cannot successfully transform into worker ants and reproductive ants. Given the above mechanism of action, the growth process of insects is inhibited to achieve the purpose of insect extermination.

[0034] However, S-methoprene in the soil, especially under ultraviolet light, will quickly degrade into naturally occurring acetic acid compounds, and eventually decompose into carbon dioxide and water. Once the S-methoprene in the bait is degraded before being eaten by foraging worker ants, the bait loses its function of interfering with the emergence of young ants.

[0035] Based on the above findings, the applicant has conducted a large number of experiments and studies and found that the bait prepared by combining wheat bran, maltose, S-methoprene and other necessary components has a better control effect on red imported fire ants and a longer control effect time than other baits containing S-methoprene. The present application is based on this.

[0036] The following examples further illustrate the present invention in detail. In the following examples, unless otherwise specified, all raw materials used are common commercial products.

[0037] Examples 1 to 6 and Comparative Examples 1 to 4 are all for preparing solid baits containing insect growth regulators, and the preparation steps are the same, the only difference being the composition and addition amount of each component. The specific weight parts of each component refer to each example and comparative example. The specific preparation steps are as follows:

[0038] Maltose, oil, insect growth regulator, protein source and preservative are weighed respectively and dissolved with acetone to fully mix all components. After the acetone is completely volatilized, wheat bran is added and mixed evenly to obtain the solid bait containing the insect growth regulator.

[0039] Example 1

[0040] The solid bait provided in Example 1 comprises 127.5 g of wheat bran, 20 g of maltose, 20 g of soybean oil, 0.5 g of S-methoprene, 30 g of insect powder and 2 g of propylparaben.

[0041] Example 2

[0042] The solid bait provided in Example 2 comprises 134 g of wheat bran, 30 g of maltose, 10 g of soybean oil, 1 g of S-methoprene, 20 g of bone meal, and 5 g of sodium benzoate.

[0043] Example 3

[0044] The solid bait provided in Example 3 comprises 133 g of wheat bran, 25 g of maltose, 16 g of soybean oil, 2 g of S-methoprene, 20 g of insect powder and 4 g of potassium sorbate.

[0045] Example 4

[0046] The solid bait provided in Example 4 comprises 130 g of wheat bran, 20 g of maltose, 16 g of soybean oil, 4 g of S-methoprene, 25 g of bone meal, and 5 g of sodium benzoate.

[0047] Example 5

[0048] The solid bait provided in Example 5 comprises 120 g of wheat bran, 20 g of maltose, 20 g of peanut oil, 6 g of S-methoprene, 30 g of insect powder, and 4 g of sodium benzoate.

[0049] Example 6

[0050] The solid bait provided in Example 5 comprises 140 g of wheat bran, 20 g of maltose, 19.8 g of rapeseed oil, 0.2 g of S-methoprene, 10 g of bone meal, and 10 g of sodium benzoate.

[0051] Comparative Example 1

[0052] The solid bait provided in Comparative Example 1 comprises 130 g corn flour, 20 g maltose, 16 g soybean oil, 4 g S-methoprene, 25 g bone meal and 5 g sodium benzoate.

[0053] Comparative Example 2

[0054] The solid bait provided in Comparative Example 2 comprises 130 g soybean powder, 20 g maltose, 16 g soybean oil, 4 g S-methoprene, 25 g bone meal and 5 g sodium benzoate.

[0055] Comparative Example 3

[0056] The solid bait provided in Comparative Example 3 comprises 130 g of wheat flour, 20 g of maltose, 16 g of soybean oil, 4 g of S-methoprene, 25 g of bone meal and 5 g of sodium benzoate.

[0057] Comparative Example 4

[0058] The solid bait provided in Comparative Example 4 comprises 130 g of bread crumbs, 20 g of maltose, 16 g of soybean oil, 4 g of S-methoprene, 25 g of bone meal and 5 g of sodium benzoate.

[0059] Performance testing

[0060] 1. Lure test

[0061] This experiment is to verify the attraction of raw materials from different sources to red fire ants. The sources are mainly divided into three categories, namely animal-based materials, plant-based materials and sugar-based materials. All materials are defatted materials.

[0062] The method of the luring test is as follows: three different red fire ant nests are selected, which are used for the luring test of raw materials from the above three sources respectively. 10g of each raw material is put into 50ml test tubes respectively. The test tubes of the same type of raw materials face the same ant nest and are arranged radially. The distance between two adjacent test tubes is equal, and the test tube mouth is 10cm away from the ant nest. Before starting the test, gently move the ant nest to make the red fire ants move, start the timing for 60 minutes, observe the number of red fire ants in the test tube after the time is up, and take pictures to identify the number, thereby determining the attractant power.

[0063] The specific test results are shown in Tables 1 to 3.

[0064] Table 1

[0065]

[0066] Table 2

[0067]

[0068] Table 3

[0069]

[0070] It can be seen from the data in Tables 1 to 3 that wheat bran, bone meal and maltose have the greatest attraction to red fire ants. Therefore, using wheat bran, bone meal and maltose as raw materials in solid baits can greatly increase the attraction to red fire ants and be more conducive to the prevention and control of red fire ants.

[0071] 2. Prevention effect test

[0072] Eleven red fire ant nest test areas were selected, and the interval between adjacent test areas was more than 20 meters. All test areas had not been treated with ant nest control in the first four months. Each test area contained 10 active ant nests, and all ant nests were of similar size. The solid baits prepared in Examples 1 to 6 and Comparative Examples 1 to 4 were placed in 10 of the test areas, and 20g of solid bait was placed around each nest in each test area. The placement position was uniformly placed within a radiation range of 80 to 120cm from the center of the red fire ant nest. No bait was placed in another test area, which served as a blank control group. The number of active ant nests in each test area was investigated once 1, 3, 5, 7, 9, 11 and 13 weeks after the solid bait was placed, and the control effect of the active ant nest was calculated. The specific results are shown in Table 4.

[0073] An active ant nest is judged by the following method: use a small wooden stick to slightly damage the surface of the ant nest. If more than 3 red fire ants come out within 60 seconds, it is judged as an active ant nest; if less than 3, it is judged as an inactive ant nest.

[0074] Active ant nest control effect (%) = [1-(M0×T M1) / (T M0×M1)]×100%;

[0075] Wherein, M0 represents the number of active ant nests in the control area before application of pesticides; TM1 represents the number of active ant nests in the test area after application of pesticides; M1 represents the number of active ant nests in the control area after application of pesticides; and TM0 represents the number of active ant nests in the test area before application of pesticides.

[0076] Table 4

[0077] 1 week 3 weeks 5 weeks 7 weeks 9 weeks 11 weeks 13 weeks Example 1 0 10％ 40％ 70％ 80％ 80％ 80％ Example 2 0 10％ 50％ 80％ 90％ 90％ 90％ Example 3 0 10％ 50％ 80％ 90％ 90％ 90％ Example 4 0 10％ 40％ 90％ 90％ 90％ 90％ Example 5 0 10％ 40％ 80％ 80％ 80％ 80％ Example 6 0 10％ 30％ 50％ 60％ 60％ 70％ Comparative Example 1 0 10％ 30％ 50％ 50％ 50％ 50％ Comparative Example 2 0 10％ 30％ 50％ 50％ 50％ 50％ Comparative Example 3 0 10％ 30％ 50％ 50％ 50％ 50％ Comparative Example 4 0 10％ 30％ 50％ 50％ 50％ 50％ Blank control 0 0 0 0 0 0 0

[0078] It can be seen from the results in Table 4 that the solid baits of Examples 3 and 4 have control effects of more than 90% after being put in for 9 weeks, indicating that the formulations of Examples 3 and 4 have excellent control effects on red fire ants. After 1 week of application, the solid baits of Examples 1 to 6 and Comparative Examples 1 to 4 all had a control effect of 0, indicating that there was no reduction in the number of active ant nests within 1 week, and the solid baits had not yet achieved the effect of killing ants; after 3 weeks of application, the solid baits of Examples 1 to 6 and Comparative Examples 1 to 4 showed control effects, indicating that some active ant nests were beginning to die; and after 7 weeks of application, the control effect of the solid bait of Example 1 reached more than 70%, and the control effect of the solid baits of Examples 2, 3, 4, and 5 reached more than 80%; after 9 weeks of application, the control effects of the solid baits of Examples 2, 3, and 4 reached 90%, respectively, the control effect of the solid baits of Examples 1 and 5 reached 80%, and the control effect of the solid bait of Example 6 reached 60%; and after 9 weeks, the control effect of the solid baits of Examples 2, 3, and 4 still remained above 90%. After 7 weeks, the control effect of the solid baits of Comparative Examples 1, 2, 3, and 4 remained at 50%, indicating that their control effects were average. It can be seen that the control effects of the solid baits of Examples 2, 3, and 4 are better than those of Examples 1, 5, and 6, and the control effects of the solid baits of Examples 1 to 6 are significantly higher than those of Comparative Examples 1 to 4 and the blank control group.

[0079] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A solid bait containing an insect growth regulator, characterized in that: The invention comprises the following components in parts by weight: 60-70 parts of wheat bran, 10-15 parts of maltose, 5-10 parts of oil, 0.1-3 parts of insect growth regulator, 5-15 parts of protein source, and 1-5 parts of preservative, wherein the insect growth regulator is S-methoprene; The preparation method comprises: weighing maltose, oil, insect growth regulator, protein source and preservative respectively, dissolving them in volatile solvent, mixing all components thoroughly, adding wheat bran and mixing evenly after the volatile solvent is completely volatilized, and obtaining the solid bait containing insect growth regulator.

2. The solid bait containing an insect growth regulator according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 63.75-67 parts of wheat bran, 10-15 parts of maltose, 5-10 parts of oil, 0.25-2 parts of insect growth regulator, 10-15 parts of protein source and 1-2.5 parts of preservative.

3. The solid bait containing an insect growth regulator according to claim 1, characterized in that: The protein source is bone meal or insect meal.

4. The solid bait containing an insect growth regulator according to claim 1, characterized in that: The oil is a combination of one or more of soybean oil, peanut oil, rapeseed oil, sesame oil, corn oil, cottonseed oil and wheat germ oil.

5. The solid bait containing an insect growth regulator according to claim 1, characterized in that: The preservative is a combination of one or more of sodium benzoate, potassium sorbate and propylparaben.

6. The method for preparing a solid bait containing an insect growth regulator according to any one of claims 1 to 5, characterized in that: include: Maltose, oil, insect growth regulator, protein source and preservative are weighed respectively and dissolved in a volatile solvent to fully mix all the components. After the volatile solvent is completely evaporated, wheat bran is added and mixed evenly to obtain the solid bait containing the insect growth regulator.

7. The method for preparing a solid bait containing an insect growth regulator according to claim 6, characterized in that: The volatile solvent includes acetone, n-hexane or n-butanol.

8. Use of a solid bait containing an insect growth regulator as claimed in any one of claims 1 to 5, characterized in that: The solid bait is used for preventing and controlling red fire ants, and the solid bait is evenly placed within a radiation range of 80 to 120 cm from the center of the red fire ant nest.

9. The use of the solid bait containing an insect growth regulator according to claim 8, characterized in that: The solid bait is put in an amount of 10-30 g / ant nest.

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