A microbial fermented skin-activating oil and its application

Abstract

The invention discloses a microbial fermentation skin-rejuvenating oil and its application. The microbial fermentation skin-rejuvenating oil, by mass, includes: 10-20 parts of fennel, 10-25 parts of safflower, 10-20 parts of cinnamon, 15-20 parts of wormwood, 5-15 parts of cloves, 10-20 parts of round-leaf angelica root, 10-20 parts of Chuanxiong, 5-12 parts of sandalwood, 5-15 parts of microbial fermentation freeze-dried powder, and 100-300 parts of water. The preparation method of the Chinese herbal medicine components in the microbial fermentation skin-rejuvenating oil includes: taking fennel, safflower, cinnamon, wormwood, cloves, round-leaf angelica root, Chuanxiong and sandalwood, washing and drying, crushing and mixing together to obtain a Chinese herbal medicine mixture; mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, performing microbial fermentation to obtain a fermentation solution; sterilizing the fermentation solution, centrifuging, and standing, taking the supernatant to obtain the microbial fermentation skin-rejuvenating oil. The application of microbial fermented skin revitalizing oil is to apply the microbial fermented skin revitalizing oil in the field of skin care. The invention has the effects of anti-aging, skin tightening and body shaping.

Description

Technical Field

[0001] The invention relates to the field of skin care products, in particular to a microbial fermented skin revitalizing oil and application thereof. Background Art

[0002] China is the birthplace of Chinese herbal medicine, and there are about 12,000 kinds of medicinal plants in China. The in-depth exploration, research and summary of Chinese herbal medicine and traditional Chinese medicine by ancient sages have made Chinese herbal medicine the most widely recognized and applied.

[0003] Some Chinese herbal medicines have the effect of improving complexion and moisturizing the skin, but the current research on Chinese herbal medicines in the field of skin care is still relatively superficial. In addition, in the field of skin care, Chinese herbal medicines are mostly crushed or fermented with yeast powder for skin care. There is currently no effective method to release the active ingredients in Chinese herbal medicines to achieve the purpose of improving skin. Summary of the invention

[0004] Based on this, the object of the present invention is to provide a microbial fermentation skin revitalizing oil and its application.

[0005] In a first aspect, a microbial fermentation revitalizing oil comprises, by weight: 10-20 parts of fennel, 10-25 parts of safflower, 10-20 parts of cinnamon, 15-20 parts of mugwort, 5-15 parts of cloves, 10-20 parts of round-leaf angelica root, 10-20 parts of chuanxiong, 5-12 parts of sandalwood, 5-15 parts of microbial fermentation freeze-dried powder, and 100-300 parts of water.

[0006] The microbial fermentation skin revitalizing oil of the present invention adopts fennel, safflower, cinnamon, wormwood, clove, round-leaf angelica root, Chuanxiong and sandalwood as main raw materials, and utilizes microbial fermentation freeze-dried powder for fermentation, so as to release ketone compounds and acid compounds in fennel, a large amount of flavonoid compounds in safflower, ethyl cinnamate and cinnamic acid in cinnamon, flavonoid compounds and triterpenoid active substances in wormwood, eugenol in clove, phenolic compounds in round-leaf angelica root, organic acids such as vanillic acid in Chuanxiong, and ketone compounds in sandalwood. These effective ingredients can promote the synthesis of human protein, activate collagen, enhance the division and regeneration ability of cells, and improve the activity and metabolism of cells, thereby playing the effects of anti-aging and skin tightening and shaping.

[0007] In one embodiment, the above technical scheme includes, by weight: 15-20 parts of fennel, 15-20 parts of safflower, 15-20 parts of cinnamon, 15-20 parts of mugwort, 5-10 parts of cloves, 15-20 parts of round-leaf angelica roots, 15-20 parts of Chuanxiong, 5-8 parts of sandalwood, 5-10 parts of microbial fermentation freeze-dried powder, and 150-200 parts of water.

[0008] In one embodiment of the above technical solution, the method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0009] Take fennel, safflower, cinnamon, mugwort, clove, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind and mix them together to obtain a Chinese herbal medicine mixture;

[0010] After mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, the mixture is subjected to microbial fermentation to obtain a fermentation solution;

[0011] The fermentation solution is sterilized, centrifuged, and allowed to stand, and then the supernatant is taken to obtain the microbial fermentation skin revitalizing oil.

[0012] In one embodiment of the above technical solution, the strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

[0013] In one embodiment of the above technical solution, the content of Lactococcus lactis JYLL-60 is ≥ 10 billion cfu / g.

[0014] In one implementation of the above technical solution, fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood are taken, washed and dried, and then crushed together to 100-200 meshes and mixed.

[0015] In one embodiment of the above technical scheme, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, the pH value is adjusted to 6.5-7, the temperature is adjusted to 30-35°C, and the microbial fermentation is carried out at 200-350r / min for 48-64h to obtain a fermentation solution.

[0016] In one implementation of the above technical solution, the fermentation solution is sterilized at 110-120° C. for 20-30 minutes, centrifuged, and allowed to stand, and then the supernatant is taken to obtain the microbial fermentation skin revitalizing oil.

[0017] In a second aspect, a use of a microbial fermented skin revitalizing oil is provided, wherein any of the microbial fermented skin revitalizing oils described above is used in the field of skin care.

[0018] In one embodiment of the above technical solution, the application method includes: using an ultrasonic introduction device to introduce the microbial fermentation skin revitalizing oil into the skin.

[0019] Compared with the prior art, the microbial fermented skin revitalizing oil and its application of the present invention adopt fennel, safflower, cinnamon, mugwort, clove, round-leaf angelica root, Chuanxiong and sandalwood as main raw materials, and ferment by microbial fermentation freeze-dried powder, which can release ketone compounds and acid compounds in fennel, a large number of flavonoids in safflower, ethyl cinnamate and cinnamic acid in cinnamon, flavonoids and triterpenoid active substances in mugwort, eugenol in cloves, phenolic compounds in round-leaf angelica root, organic acids such as vanillic acid in Chuanxiong, and ketone compounds in sandalwood. These active ingredients can promote the synthesis of human protein, activate collagen, enhance cell division and regeneration ability, and improve cell activity and metabolism, thereby playing an anti-aging and skin tightening and shaping effect on the skin.

[0020] For better understanding and implementation, the present invention is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 These are comparison photos of volunteer 1 before and after the first phase of the trial.

[0022] Figure 2 These are comparison photos of volunteer 1 before and after the second phase of the trial.

[0023] Figure 3 These are comparison photos of Volunteer 2 before and after the first phase of the trial.

[0024] Figure 4 These are comparison photos of Volunteer 2 before and after the second phase of the trial.

[0025] Figure 5 These are comparison photos of volunteer 4 before and after the test.

[0026] Figure 6 These are comparison photos of Volunteer 5 before and after the first phase of the trial.

[0027] Figure 7 These are comparison photos of Volunteer 5 before and after the second phase of the trial. DETAILED DESCRIPTION

[0028] The directional terms such as up, down, left, right, front, back, front, back, top, bottom, etc. mentioned or may be mentioned in this specification are defined relative to their structure and are relative concepts. Therefore, they may change accordingly according to their different locations and different usage conditions. Therefore, these or other directional terms should not be interpreted as restrictive terms.

[0029] The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present disclosure. Instead, they are merely examples of embodiments consistent with some aspects of the present disclosure.

[0030] The terms used in this disclosure are only for the purpose of describing specific embodiments and are not intended to limit the disclosure. The singular forms of "a", "the" and "the" used in this disclosure are also intended to include plural forms, unless the context clearly indicates other meanings. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more associated listed items.

[0031] In a first aspect, a microbial fermentation revitalizing oil comprises, by weight: 10-20 parts of fennel, 10-25 parts of safflower, 10-20 parts of cinnamon, 15-20 parts of mugwort, 5-15 parts of cloves, 10-20 parts of round-leaf angelica root, 10-20 parts of chuanxiong, 5-12 parts of sandalwood, 5-15 parts of microbial fermentation freeze-dried powder, and 100-300 parts of water.

[0032] In one embodiment, the composition comprises, by weight: 15-20 parts of fennel, 15-20 parts of safflower, 15-20 parts of cinnamon, 15-20 parts of mugwort, 5-10 parts of cloves, 15-20 parts of round-leaf angelica roots, 15-20 parts of chuanxiong, 5-8 parts of sandalwood, 5-10 parts of microbial fermentation freeze-dried powder, and 150-200 parts of water.

[0033] Ketone compounds and acid compounds in fennel, a large number of flavonoids in safflower, ethyl cinnamate and cinnamic acid in cinnamon, flavonoids and triterpenoid active substances in mugwort, eugenol in cloves, phenolic compounds in angelica root, organic acids such as vanillic acid in Chuanxiong, and ketone compounds in sandalwood are all compounds beneficial to the skin. These active ingredients can promote the synthesis of human protein, activate collagen, enhance cell division and regeneration ability, and improve cell activity and metabolism.

[0034] Microbial fermentation freeze-dried powder is easy to purchase and does not need to be configured separately, thus reducing costs.

[0035] In one embodiment, the strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60. Lactococcus lactis is a wall-broken strain and is a fungus with excellent fermentation ability. During fermentation, it can effectively release active ingredients in Chinese herbal medicine ingredients.

[0036] Preferably, the content of Lactococcus lactis JYLL-60 is ≥ 10 billion cfu / g.

[0037] The lactococcus lactis JYLL-60 in the present application was purchased from Shandong Nuoran Special Medical Formula Food Co., Ltd. The remaining Chinese herbal medicine raw materials are conventional commercial products. Purified water is preferably used as water.

[0038] In one embodiment, the method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0039] Step 101, taking fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, washing and drying them, and then crushing and mixing them together to obtain a Chinese herbal medicine mixture.

[0040] Preferably, fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood are washed and dried, then crushed together to 100-200 meshes and mixed.

[0041] Step 102, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, perform microbial fermentation to obtain a fermentation solution.

[0042] Preferably, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, the pH value is adjusted to 6.5-7, the temperature is adjusted to 30-35°C, and the microbial fermentation is carried out at 200-350r / min for 48-64h to obtain a fermentation solution.

[0043] Lactococcus lactis JYLL-60 prefers a slightly acidic environment, and the suitable growth temperature is around 30°C. Fermentation under these conditions can maximize the decomposition and fermentation capabilities of Lactococcus lactis JYLL-60 and fully release the active ingredients in Chinese herbal medicines.

[0044] Step 103, sterilize the fermentation solution, centrifuge it, let it stand, and then take the supernatant to obtain the microbial fermentation skin revitalizing oil.

[0045] Preferably, the fermentation solution is sterilized at 110-120° C. for 20-30 min, centrifuged, and allowed to stand, and then the supernatant is collected to obtain an oily microbial fermented skin-revitalizing oil.

[0046] Example 1

[0047] The microbial fermentation skin revitalizing oil comprises, by weight: 10 parts of fennel, 10 parts of safflower, 10 parts of cinnamon, 15 parts of wormwood, 5 parts of cloves, 10 parts of round-leaved angelica root, 10 parts of chuanxiong, 5 parts of sandalwood, 5 parts of microbial fermentation freeze-dried powder, and 100 parts of water. The strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

[0048] The method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0049] Step 101, take fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind them together to 200 mesh, and mix them.

[0050] Step 102, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, adjusting the pH value to 6.5, adjusting the temperature to 35°C, and performing microbial fermentation at 350 r / min for 48 hours to obtain a fermentation solution.

[0051] Step 103, sterilize the fermentation solution at 110°C for 20 minutes, centrifuge and let stand, and then take the supernatant to obtain an oily microbial fermentation skin revitalizing oil.

[0052] Example 2

[0053] The microbial fermentation skin revitalizing oil comprises, by weight: 20 parts of fennel, 25 parts of safflower, 20 parts of cinnamon, 20 parts of wormwood, 15 parts of cloves, 20 parts of round-leaved angelica root, 20 parts of chuanxiong, 12 parts of sandalwood, 15 parts of microbial fermentation freeze-dried powder, and 300 parts of water. The strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

[0054] The method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0055] Step 101, take fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind them together to 200 mesh, and mix them.

[0056] Step 102, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, adjusting the pH value to 6.5, adjusting the temperature to 30°C, and performing microbial fermentation at 200 r / min for 48 hours to obtain a fermentation solution.

[0057] Step 103, sterilize the fermentation solution at 110°C for 30 minutes, centrifuge and let stand, and then take the supernatant to obtain an oily microbial fermentation skin-activating oil.

[0058] Example 3

[0059] The microbial fermentation skin revitalizing oil comprises, by weight: 15 parts of fennel, 18 parts of safflower, 13 parts of cinnamon, 17 parts of wormwood, 12 parts of cloves, 16 parts of round-leaved angelica root, 18 parts of chuanxiong, 8 parts of sandalwood, 9 parts of microbial fermentation freeze-dried powder, and 180 parts of water. The strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

[0060] The method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0061] Step 101, take fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind them together into 100 mesh, and mix them.

[0062] Step 102, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, adjusting the pH value to 7, adjusting the temperature to 35°C, and performing microbial fermentation at 300 r / min for 64 hours to obtain a fermentation solution.

[0063] Step 103, sterilize the fermentation solution at 110°C for 30 minutes, centrifuge and let stand, and then take the supernatant to obtain an oily microbial fermentation skin-activating oil.

[0064] Example 4

[0065] The microbial fermentation skin revitalizing oil comprises, by weight: 15 parts of fennel, 15 parts of safflower, 15 parts of cinnamon, 15 parts of wormwood, 5 parts of cloves, 15 parts of round-leaved angelica root, 15 parts of chuanxiong, 5 parts of sandalwood, 5 parts of microbial fermentation freeze-dried powder, and 150 parts of water. The strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

[0066] The method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0067] Step 101, take fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind them together into 100 mesh, and mix them.

[0068] Step 102, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, adjusting the pH value to 7, adjusting the temperature to 30°C, and performing microbial fermentation at 300 r / min for 64 hours to obtain a fermentation solution.

[0069] Step 103, sterilize the fermentation solution at 120°C for 30 minutes, centrifuge and let stand, and then take the supernatant to obtain an oily microbial fermentation skin-activating oil.

[0070] Example 5

[0071] The microbial fermentation skin revitalizing oil comprises, by weight: 20 parts of fennel, 20 parts of safflower, 20 parts of cinnamon, 20 parts of wormwood, 10 parts of cloves, 20 parts of round-leaved angelica root, 20 parts of chuanxiong, 8 parts of sandalwood, 10 parts of microbial fermentation freeze-dried powder, and 200 parts of water. The strain in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

[0072] The method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises:

[0073] Step 101, take fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind them together into 100 mesh, and mix them.

[0074] Step 102, after mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, adjusting the pH value to 6.5, adjusting the temperature to 30°C, and performing microbial fermentation at 350 r / min for 64 hours to obtain a fermentation solution.

[0075] Step 103, sterilize the fermentation solution at 110°C for 30 minutes, centrifuge and let stand, and then take the supernatant to obtain an oily microbial fermentation skin-activating oil.

[0076] In the second aspect, a microbial fermented revitalizing oil is used in the field of skin care.

[0077] In one embodiment, the application method comprises: introducing the microbial fermentation skin revitalizing oil into the skin using an ultrasonic introduction device.

[0078] Test Example 1

[0079] 45 volunteers aged between 28 and 58 were recruited successively (not simultaneously), including 18 male volunteers and 27 female volunteers. These 45 volunteers generally had problems such as skin aging, sagging, and roughness.

[0080] 45 volunteers were randomly divided into 5 groups, with 9 volunteers in each group. The 5 groups used the microbial fermented skin revitalizing oil of Examples 1-5 respectively. For example, Group 1 used the microbial fermented skin revitalizing oil of Example 1, and so on.

[0081] How to use: After cleansing the skin, apply the microbial fermentation revitalizing oil evenly on the face, and use an ultrasonic introduction device to introduce it into the face for 20 minutes, once a day, for 30 days.

[0082] Results and statistical methods: Collect facial photos of each volunteer without makeup before and after the test and record the skin condition. According to the uniform distribution of age, randomly select 1 volunteer from the 9 volunteers in each group, and a total of 5 volunteers are selected. The 5 volunteers are:

[0083] Volunteer 1: Female, 32 years old;

[0084] Volunteer 2: male, 30 years old;

[0085] Volunteer 3: male, 41 years old;

[0086] Volunteer 4: Female, 55 years old;

[0087] Volunteer 5: Female, 62 years old.

[0088] Photos of the five volunteers before and after the test were collected, and their skin conditions before and after the test were recorded.

[0089] The test results are shown in the table below.

[0090]

[0091] Table 1

[0092] As shown in Table 1, after using the microbial fermentation revitalizing oil of the present invention for skin care, the volunteers' skin became more delicate, elastic, and their cheeks became firmer, and some volunteers also had whitening effects on their skin.

[0093] Test Example 2

[0094] After using it for 30 days and achieving obvious improvement, Volunteer 1, Volunteer 2, Volunteer 4 and Volunteer 5 clearly expressed their willingness to continue using the microbial fermented skin revitalizing oil of the present invention for skin care.

[0095] Record the changes in skin condition of volunteers 1, 2, 4 and 5 during the use period, see Figure 1-Figure 7 and the table below.

[0096]

[0097]

[0098] Table 2

[0099] Depend on Figure 1-Figure 7 As shown in Table 2, after further skin care with the microbial fermentation revitalizing oil of the present invention, the volunteers' skin became more delicate, elastic, and their cheeks became firmer, and their complexion became significantly better. Volunteer 5 also showed a significant reduction in forehead wrinkles and cheek wrinkles.

[0100] Compared with the prior art, the microbial fermented skin revitalizing oil and its application of the present invention adopt fennel, safflower, cinnamon, mugwort, clove, round-leaf angelica root, Chuanxiong and sandalwood as main raw materials, and ferment by microbial fermentation freeze-dried powder, which can release ketone compounds and acid compounds in fennel, a large number of flavonoids in safflower, ethyl cinnamate and cinnamic acid in cinnamon, flavonoids and triterpenoid active substances in mugwort, eugenol in cloves, phenolic compounds in round-leaf angelica root, organic acids such as vanillic acid in Chuanxiong, and ketone compounds in sandalwood. These active ingredients can promote the synthesis of human protein, activate collagen, enhance cell division and regeneration ability, and improve cell activity and metabolism, thereby playing an anti-aging and skin tightening and shaping effect on the skin.

[0101] The above-mentioned embodiments only express several implementation methods of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A microbial fermented skin revitalizing oil, characterized in that: The composition comprises, by weight: 10-20 parts of fennel, 10-25 parts of safflower, 10-20 parts of cinnamon, 15-20 parts of mugwort, 5-15 parts of cloves, 10-20 parts of round-leaf angelica root, 10-20 parts of chuanxiong, 5-12 parts of sandalwood, 5-15 parts of microbial fermentation freeze-dried powder, and 100-300 parts of water; The method for preparing the Chinese herbal medicine components in the microbial fermentation skin revitalizing oil comprises: Take fennel, safflower, cinnamon, mugwort, clove, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind and mix them together to obtain a Chinese herbal medicine mixture; After mixing the microbial fermentation freeze-dried powder, water and the Chinese herbal medicine mixture, adjusting the pH value to 6.5-7, adjusting the temperature to 30-35° C., and performing microbial fermentation at 200-350 r / min for 48-64 hours to obtain a fermentation solution; The fermentation solution is sterilized, centrifuged, and allowed to stand, and then the supernatant is taken to obtain the microbial fermentation skin-activating oil; The bacterial species in the microbial fermentation freeze-dried powder is Lactococcus lactis JYLL-60.

2. The microbial fermented skin revitalizing oil according to claim 1, characterized in that: Calculated by weight, the invention comprises: 15-20 parts of fennel, 15-20 parts of safflower, 15-20 parts of cinnamon, 15-20 parts of mugwort, 5-10 parts of cloves, 15-20 parts of round-leaf angelica roots, 15-20 parts of chuanxiong, 5-8 parts of sandalwood, 5-10 parts of microbial fermentation freeze-dried powder and 150-200 parts of water.

3. The microbial fermented skin revitalizing oil according to any one of claims 1 to 2, characterized in that: The content of Lactococcus lactis JYLL-60 is ≥10 billion cfu / g.

4. The microbial fermented skin revitalizing oil according to any one of claims 1 to 2, characterized in that: Take fennel, safflower, cinnamon, mugwort, cloves, round-leaf angelica root, chuanxiong and sandalwood, wash and dry them, grind them together to 100-200 mesh, and mix them.

5. The microbial fermented skin revitalizing oil according to any one of claims 1 to 2, characterized in that: The fermentation solution is sterilized at 110-120° C. for 20-30 minutes, centrifuged, and allowed to stand, and then the supernatant is taken to obtain the microbial fermentation skin revitalizing oil.

6. An application of microbial fermented skin revitalizing oil, characterized in that: The microbial fermented skin revitalizing oil according to any one of claims 1 to 5 is applied in the field of skin care.

7. The use of the microbial fermentation skin revitalizing oil according to claim 6, characterized in that: The application method comprises: using an ultrasonic introduction instrument to introduce the microbial fermentation skin revitalizing oil into the skin.

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