Staphylococcus aureus fluorescence detection method based on navigation type DNA walker and fluorescence regulation mechanism

Abstract

This invention relates to a fluorescence detection method for Staphylococcus aureus based on a navigation-type DNA walker and a fluorescence regulation mechanism. It utilizes a dual-enzyme recognition reaction system of exonuclease I and exonuclease III to identify and distinguish samples containing / without Staphylococcus aureus. Samples containing Staphylococcus aureus generate an efficient fuel chain in the dual-enzyme recognition reaction system, causing the DNA walker to shift along its trajectory, further triggering fluorescence resonance energy transfer, ultimately obtaining an amplified fluorescence signal correlated with the target concentration. The concentration of Staphylococcus aureus in the contaminated sample is determined based on the relationship between fluorescence intensity and Staphylococcus aureus concentration. This invention improves the controllability and efficiency of the DNA walker, reducing the risk of derailment and interruption. The detection range is 9-90 CFU / mL. ‑1 Within its detection range, the accuracy of quantitative detection reaches 100%, and the reproducibility and recovery rate exceed 98%, capable of detecting substances containing 2 CFU / mL. ‑1 The sample of Staphylococcus aureus.

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