A pathological imaging system and imaging method based on fluorescence immunoassay technology

Abstract

The present application relates to the technical field of pathological tissue imaging, and discloses a pathological imaging system and an imaging method based on fluorescence immunotechnology, including: an immunodetection kit: including biomarkers and fluorescent materials, for clinical pathological tissue processing and staining; a polymer cell sieve: for screening and embedding clinical pathological tissues; a photographing platform: for photographing the clinically pathological tissues after staining to form pathological pictures; an AI film reading system: for reading the pathological pictures and completing the annotation of the pathological pictures. It can reduce labor and at the same time reduce the dependence on the "film reading experience" of pathologists; it does not use the paraffin embedding technique, thus shortening the time; fluorescence immunostaining improves the accuracy of AI pathological film reading; multiple biomarkers can be stained at one time, reducing the usage amount of tissue samples.

Description

Technical Field

[0001] This application relates to the technical field of medical pathological imaging, and particularly to a pathological imaging system and imaging method based on fluorescence immunoassay technology. Background Art

[0002] Existing imaging technologies are not convenient for junior pathology doctors to read pathological results, and require senior pathology doctors to read the films; by directly lysing tissues, it solves the problem that "tumor heterogeneity" cannot be detected using paraffin sections in conventional pathology and immunohistopathology;

[0003] In immunohistochemical pathology, only 1 biomarker can be detected each time (or for each paraffin section), increasing the usage of pathological tissues (especially in puncture tissues, etc.);

[0004] Moreover, the recognition degree of conventional pathology and immunohistochemical staining pictures is low, and senior pathology doctors are required to complete the annotation, resulting in high labor costs. Summary of the Invention

[0005] To solve the above technical problems, this application provides a pathological imaging system and imaging method based on fluorescence immunoassay technology, which reduces labor and simultaneously reduces the dependence on "film reading experience" of pathology doctors; does not use paraffin embedding technology, shortening the time; fluorescence immunoassay staining improves the accuracy of AI pathological film reading;

[0006] Multiple biomarkers are stained at one time, reducing the usage of tissue samples.

[0007] A pathological imaging system based on fluorescence immunoassay technology provided by this application adopts the following technical solutions:

[0008] A pathological imaging system based on fluorescence immunoassay technology includes:

[0009] Immunodetection kit: including biomarkers and fluorescent materials, used for clinical pathological tissue processing and staining;

[0010] Polymer cell sieve: used for screening and embedding clinical pathological tissues;

[0011] Photographing platform: used for photographing the stained clinical pathological tissues to form pathological pictures;

[0012] AI film reading system: used for reading the pathological pictures and completing the annotation of the pathological pictures.

[0013] By adopting the above technical solutions: This system collaborates to conduct automated experiments, generates "pathological pictures" in the order of "sample processing", "sample staining", and "picture reading", and then completes the film reading through the "AI system" to complete the annotation work of the pathological pictures.

[0014] Optionally, the immunoassay kit includes a box body, in which a biological label and a fluorescent material are provided, and the fluorescent material is an organic fluorescent material or an inorganic fluorescent material.

[0015] By adopting the above technical solution: the clinical pathological tissue can be stained and labeled by the biological label and the fluorescent material, which is convenient for the recognition of later imaging reading.

[0016] Optionally, the polymer cell sieve includes a sieve body, a sieve mesh is provided at the lower part of the sieve body, and an elastic component is provided inside the sieve body above the sieve mesh.

[0017] By adopting the above technical solution: by arranging the elastic component, the disturbance of the bottom cells can be realized, which is convenient for embedding and staining.

[0018] Optionally, the sieve body is in a conical barrel shape, and the elastic component includes an elastic sheet arranged inside the sieve body and a driving member for driving the elastic sheet to act.

[0019] By adopting the above technical solution: by arranging the elastic sheet, the cells above the sieve mesh can be disturbed, which is convenient for screening and embedding.

[0020] Optionally, the elastic sheet includes a driving elastic part and a main elastic part. Both the driving elastic part and the main elastic part are arc-shaped, and the radian of the driving elastic part is smaller than that of the main elastic part. One end of the main elastic part is connected to the inside of the sieve body, the other end is connected to the driving elastic part, and the other end of the driving elastic part is connected to the inner wall of the sieve body.

[0021] By adopting the above technical solution: when the driving member drives the elastic sheet, it first contacts the driving elastic part. While the driving elastic part is moving, it drives the main elastic part to move. When the driving member moves upward, the driving elastic part and the main elastic part return to their original states, which can form a side disturbance effect on the cells and is more convenient for screening and embedding.

[0022] Optionally, the driving member includes an electric telescopic rod. One end of the electric telescopic rod extending into the sieve body is connected with a driving frame, and a driving ring is connected to the outside of the driving frame. The driving ring contacts the elastic sheet.

[0023] By adopting the above technical solution: by arranging the driving frame and the driving ring, the flow direction of the cells will not be affected.

[0024] Optionally, an arc-shaped guiding part is provided on the side of the driving ring.

[0025] By adopting the above technical solution: the arc-shaped guiding part can contact the elastic sheet first during the downward movement of the driving ring. Under the action of the arc, it has a guiding effect and can avoid damage to the elastic sheet caused by hard contact.

[0026] A pathological imaging method based on fluorescence immunoassay technology provided by this application includes the following steps:

[0027] S1. Combine a biomarker with a fluorescent material to form a fluorescence immunoassay staining kit, and place the pathological tissue sample in the fluorescence immunoassay staining kit for labeling and staining;

[0028] S2. Embed the stained pathological tissue sample in a polymer cell sieve; the tissue embedded in the polymer cell sieve sequentially completes tissue digestion and cell staining to combine the biomarker on the cell with the staining reagent;

[0029] S3. Place the stained pathological tissue on a photographing platform, and use the specific light source of the fluorescent material to develop color, and complete photographing to form a pathological picture;

[0030] S4. For the pathological picture, complete the annotation of the pathological picture through an AI image reading system.

[0031] In summary, this application includes at least one of the following beneficial technical effects:

[0032] 1. Reduce labor and at the same time reduce the dependence on the "film reading experience" of pathologists; do not use paraffin embedding technology and shorten the time;

[0033] 2. Fluorescence immunoassay staining improves the accuracy of AI pathological image reading; stain multiple biomarkers at one time and reduce the usage amount of tissue samples. Description of the Drawings

[0034] Figure 1 is a schematic diagram of the system framework of an embodiment of this application;

[0035] Figure 2 is a schematic diagram of the structure of the polymer cell sieve of an embodiment of this application;

[0036] Figure 3 is a top view structure diagram of the driving member of an embodiment of this application.

[0037] Description of the reference numerals: 100, immunoassay kit; 200, polymer cell sieve; 210, sieve body; 220, sieve mesh; 230, elastic component; 231, elastic sheet; 2311, driving elastic part; 2312, main elastic part; 232, driving member; 2321, driving frame; 2322, driving ring; 2323, arc guiding part; 300, photographing platform; 400, AI image reading system. Detailed Embodiment

[0038] The following is a further detailed description of this application in combination with the attached Figures 1-3 drawings.

[0039] The embodiments of the present application disclose a pathological imaging system and an imaging method based on fluorescence immunoassay technology, specifically as follows:

[0040] Referring to Figures 1-3 , a pathological imaging system based on fluorescence immunoassay technology provided by the present application in this embodiment adopts the following technical solutions:

[0041] A pathological imaging system based on fluorescence immunoassay technology includes:

[0042] Immunodetection kit 100: including biomarkers and fluorescent materials, used for clinical pathological tissue processing and staining;

[0043] Polymer cell sieve 200: used for screening and embedding clinical pathological tissues;

[0044] Photographing platform 300: used for photographing the stained clinical pathological tissues to form pathological pictures;

[0045] AI film reading system 400: used for reading the pathological pictures and completing the annotation of the pathological pictures. This system collaborates to conduct automated experiments, generates "pathological pictures" in the order of "sample processing", "sample staining", and "picture reading", and then completes the film reading through the "AI system" to complete the annotation work of the pathological pictures.

[0046] Among them, the immunodetection kit 100 includes a box body, and biomarkers and fluorescent materials are arranged inside the box body. The fluorescent material is an organic fluorescent material or an inorganic fluorescent material. The clinical pathological tissues can be stained and marked through the biomarkers and fluorescent materials, which is convenient for the identification of later imaging and film reading.

[0047] In order to better screen and embed cells, the polymer cell sieve 200 includes a sieve body 210, a sieve mesh 220 is arranged at the lower part of the sieve body 210, and an elastic component 230 is arranged inside the sieve body 210 above the sieve mesh 220. By setting the elastic component 230, the disturbance of the bottom cells can be realized, which is convenient for embedding and staining.

[0048] Among them, the sieve body 210 is in a conical barrel shape, and the elastic component 230 includes an elastic sheet 231 arranged inside the sieve body 210 and a driving member 232 for driving the elastic sheet 231 to act. By setting the elastic sheet 231, the cells above the sieve mesh 220 can be disturbed, which is convenient for screening and embedding.

[0049] Among them, the elastic sheet 231 includes a driving elastic part 2311 and a main elastic part 2312. Both the driving elastic part 2311 and the main elastic part 2312 are arc-shaped, and the radian of the driving elastic part 2311 is smaller than that of the main elastic part 2312. One end of the main elastic part 2312 is connected to the inside of the sieve body, the other end is connected to the driving elastic part 2311, and the other end of the driving elastic part 2311 is connected to the inner wall of the sieve body 210. When the driving member 232 drives the elastic sheet 231, it first contacts the driving elastic part 2311. While the driving elastic part 2311 is moving, it drives the main elastic part 2312 to move. When the driving member 232 moves upward, the driving elastic part 2311 and the main elastic part 2312 return to their original states, which can form a side disturbance effect on the cells, making it more convenient for screening and embedding.

[0050] In this embodiment, the driving member 232 includes an electric telescopic rod. One end of the electric telescopic rod extending into the sieve body 210 is connected with a driving frame 2321. The outside of the driving frame 2321 is connected with a driving ring 2322, and the driving ring 2322 contacts the elastic member. By providing the driving frame 2321 and the driving ring 2322, the flow direction of the cells will not be affected. An arc-shaped guiding part 2323 is provided on the side of the driving ring 2322. The arc-shaped guiding part 2323 can contact the elastic sheet 231 first during the downward movement of the driving ring 2322, and has a guiding effect under the action of the arc, avoiding hard contact from damaging the elastic sheet 231.

[0051] A pathological imaging method based on fluorescence immunoassay technology provided by this application includes the following steps:

[0052] S1. Combine a biomarker with a fluorescent material to form a fluorescence immunoassay staining kit, and place the pathological tissue sample in the fluorescence immunoassay staining kit for labeling and staining; among them, the biomarker uses water-soluble quantum dots with good biocompatibility and batch stability;

[0053] S2. Embed the stained pathological tissue sample in the polymer cell sieve 200; the tissue embedded in the polymer cell sieve 200 sequentially completes tissue digestion and cell staining so that the biomarker on the cells binds to the staining reagent;

[0054] S3. Place the stained pathological tissue on the photographing platform 300, and use the specific light source of the fluorescent material to develop color, and complete photographing to form a pathological picture;

[0055] S4. For the pathological picture, through the AI film reading system 400, complete the annotation of the pathological picture.

[0056] The above are all the preferred embodiments of this application. The protection scope of this application is not limited accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A pathological imaging system based on fluorescence immunoassay technology, characterized in that, Including: Immunodetection kit (100): including biomarkers and fluorescent materials, for clinical pathological tissue processing and staining; high-molecular cell sieve (200): for screening and embedding clinical pathological tissues; photographing platform (300): for photographing the clinically pathological tissues after staining to form pathological pictures; AI film reading system (400): for reading the pathological pictures and completing the annotation of the pathological pictures. The immunodetection kit (100) includes a box body, and a biological marker and a fluorescent material are arranged inside the box body. The fluorescent material is an organic fluorescent material or an inorganic fluorescent material. The high-molecular cell sieve (200) includes a sieve body (210), a sieve mesh (220) is arranged at the lower part of the sieve body (210), and an elastic component (230) is arranged inside the sieve body (210) above the sieve mesh (220). The sieve body (210) is in a conical barrel shape. The elastic component (230) includes an elastic sheet (231) arranged inside the sieve body (210) and a driving member (232) for driving the elastic sheet (231) to act. The elastic sheet (231) includes a driving elastic part (2311) and a main elastic part (2312). Both the driving elastic part (2311) and the main elastic part (2312) are arc-shaped, and the radian of the driving elastic part (2311) is smaller than that of the main elastic part (2312). One end of the main elastic part (2312) is connected to the inside of the sieve body (210), the other end is connected to the driving elastic part (2311), and the other end of the driving elastic part (2311) is connected to the inner wall of the sieve body (210). The driving member (232) includes an electric telescopic rod. One end of the electric telescopic rod extending into the sieve body (210) is connected with a driving frame (2321), and a driving ring (2322) is connected to the outside of the driving frame (2321). The driving ring (2322) contacts the elastic sheet (231). An arc guiding part (2323) is arranged on the side of the driving ring (2322).

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