Queen bee preservation method and preservation device

Abstract

The application discloses a queen bee preservation method and a preservation device, and the queen bee preservation method is characterized in that each queen bee has its own accompanying worker bee through the establishment of a queen bee preservation group, so that the uneven feeding caused by the uneven ability of queen bees is avoided, and the maximization of production and breeding benefits is achieved; meanwhile, the establishment of the queen bee preservation group and a diversified worker bee replacement scheme can realize the common preservation of multiple queen bees in an incubator environment. In addition, an identity chip is bound to the queen bee during the queen bee preservation, so that the queen bee can be conveniently inspected and managed, and the state of the queen bee can be monitored in real time. The queen bee preservation device is designed with a honeybee living room and a honeybee isolation room, the design fully considers the social habits of honeybees, the honeybees are allowed to be familiar with each other's smell before closely contacting with honeybees of different bee groups, and the fighting of worker bees or the behavior of isolating queen bees caused by the identity recognition error is avoided; meanwhile, the honeybees are fed by adopting a dry-wet separation method, and the healthy preservation state of the honeybees is beneficial to be maintained.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of queen bee preservation, and particularly relates to a queen bee preservation method and a preservation device. BACKGROUND

[0002] In nature, the queen bee is the core of the bee colony, which can release queen pheromones to command the worker bees to work in an orderly manner, and only the queen bee can lay worker eggs to maintain or expand the colony. In bee breeding, in order to obtain higher economic benefits, queen bees with strong productivity and strong disease resistance are usually selected. Modern technology can direct the cultivation of high-quality offspring queen bees by selecting parent and maternal bees and performing artificial insemination of queen bees. In this process, artificial insemination operation can be performed only after the queen bee leaves the house and is cultured to sexual maturity, so efficient cultivation of high-quality queen bees involves the problem of how to preserve multiple queen bees.

[0003] In addition, the queen bee has a certain service life, and when the productivity and pheromone level of the queen bee gradually decrease, it is easy to cause the new house bees to decrease and the colony to weaken. Even the queen bee actively escapes or is passively excluded from the colony, and the worker bees begin to split the bees, which will cause certain economic losses if timely measures are not taken. Therefore, it is necessary to monitor the preservation of the queen bee in the colony on a daily basis, and replace the new queen bee in advance according to the service life of the queen bee in each colony.

[0004] The steps of artificial queen breeding include: selecting a strong colony as a queen breeding colony, removing the original egg-laying queen after ensuring sufficient eggs, placing the queen in a state of losing the queen, and then intervening the prepared artificial queen stand. The queen in the queen stand can produce a new generation of virgin queen after about 16 days of development. At this time, the original egg-laying queen can be put back, the cultivated virgin queen can be put into the colony that needs to be replaced, and a part of the virgin queen can be saved for replacement in case of failure of the first queen intervention. In this process, how to save the original egg-laying queen with excellent traits for a long time or save the standby virgin queen also becomes an unavoidable problem in bee breeding.

[0005] At present, the intelligentization and informatization level of bee breeding management technology in China is low, and the traditional recording method of using paper and pen or marking on the beehive is prone to information transmission errors and contains less information, which is difficult to update in time. The existing bee farm management technology cannot continuously record the identity, strain and use state of the queen bee, and there is a lack in monitoring the preservation of the queen bee and effectively guiding the production of the bee farm.

[0006] In addition, the technology for preserving queen bees commonly involves confining multiple queen bees in a columnar or frame-type queen storage frame, placing the queen storage frame in a queenless bee colony for feeding by worker bees, and then taking out the queen storage frame for use when needed. However, due to the difference in the ability of each queen bee to recruit worker bees, selective feeding by worker bees may occur when the queen bees are cultivated in this way, and the nutritional needs of some queen bees may not be met, resulting in their development lagging behind or even mass death. Secondly, when individual queen bees are taken out, the operation of taking out the queen storage frame may affect the stability of the worker bees and the remaining queen bees in the rearing colony. In addition, the rearing colony is not in a production state because there is no queen bee, and regular artificial intervention of worker bee sperms is needed to maintain the colony strength. The consumption of sperms is large in the long-term preservation, and the temperature control ability of the bee colony is insufficient to pose a challenge to the safety of the queen bee when affected by extreme outdoor weather. SUMMARY

[0007] In view of the above problems in the prior art, the purpose of the present application is to provide a method for preserving and managing queen bees, which solves the problem of selective feeding by worker bees in the existing queen bee preservation technology, leading to malnutrition, delayed development, or even mass death of some queen bees, and also solves the problem of insufficient temperature control ability of the bee colony posing a threat to the safety of the queen bee when affected by extreme outdoor weather.

[0008] The present application also designs a special queen bee preservation device for implementing the above method, which solves the problem of worker bees fighting or even isolating queen bees due to identity recognition errors, and maintains the healthy preservation state of bees from multiple angles.

[0009] To solve the above technical problems, the present application adopts the following technical solutions:

[0010] A method for preserving queen bees, comprising the following steps:

[0011] (1) Establishing a queen bee preservation colony: a queen bee preservation colony is established according to a queen bee to worker bee quantity ratio of 1:50-100, and the queen bees and worker bees are placed in a queen bee preservation device for preservation, and then the preservation device is placed in a 35℃ / 60%RH incubator for preservation; the queen bee preservation device has the function of separate cultivation of existing bees and new bees under the condition that they can contact each other's odor, and can also combine the cultivation of existing bees and new bees as needed;

[0012] (2) Feeding bees: during the preservation process, pure water needs to be added to the water storage pipe in the preservation device at regular intervals, and the cube sugar and pollen balls in the solid feeding box need to be replaced at regular intervals;

[0013] (3) Cleaning up dead bees and replacing worker bees: after a period of cultivation, when dead bees are observed, clean up the dead bees; when new worker bees are needed, anesthetize the new worker bees and place them in the storage device, separate the new worker bees from the original bees for 12-20 hours in the case of contact with each other's odor, and then combine cultivation; when the worker bees need to be replaced, anesthetize the storage device in 60% CO2, remove the queen bee and worker bees, and then place the queen bee and new worker bees into the storage device for separate cultivation, and when obvious feeding behavior appears, combine the queen bee and new worker bees for cultivation;

[0014] (4) Artificial insemination of queen bees: when the queen bee enters the sexual maturity stage, anesthetize the storage device in 60% CO2, remove the bees, and perform artificial insemination on the queen bee, and the remaining worker bees are placed back into the storage device; after the queen bee is inseminated, the queen bee is placed back into the storage device, and then the storage device is placed back into the incubator for storage.

[0015] Further, in the step (1) of establishing a queen bee storage group, when the queen bee needs to be stored for sexual maturity, one queen bee can be placed with 50 accompanying worker bees; when the queen bee needs to be stored for a long time, 100 accompanying worker bees can be placed, and the worker bees can be replaced every 30 days according to the death situation; in the selection of worker bees, young worker bees from queenless groups can be selected and placed into the container, at which time the worker bees have a higher acceptance ability for the queen bee and a shorter queen intervention time; if worker bees from queen groups that have just left the hive are selected, the worker bees need to be placed in the container before the queen bee leaves the hive, so that the worker bees are in a queenless state for a period of time, and are placed in a 35°C / 60% RH incubator environment for 24 hours before being introduced into the queen stand or the queen bee.

[0016] Further, in the step (2) of feeding the bees, the pure water is added 18 hours once for 50 worker bees with 5ml of water; the granulated sugar and pollen balls are replaced every two days to ensure freshness.

[0017] Further, it further includes a queen bee inspection management: before step (2), anesthetize the storage device in a 60% CO2 environment for 3 minutes, remove the queen bee, paste the identity chip on the back of the queen bee, and then place it back into the storage device, turn on the queen bee inspection system, scan the identity chip, and record the strain information and birth information to realize the binding of the queen bee identity; during the storage process, the inspection equipment is used to scan the storage device, monitor the physiological state and movement of the queen bee, so as to facilitate the management of the queen bee.

[0018] The queen bee preservation device for implementing the queen bee preservation method comprises a preservation device body, a cavity for the activity of bees is arranged in the preservation device body, a plurality of strip-shaped main ventilation openings are arranged on the front and rear sides of the preservation device body, and the main ventilation openings are communicated with the cavity of the preservation device body; the cavity comprises two upper and lower cavities; the upper cavity is a bee living room, and the lower cavity is a bee isolation room; the upper cavity and the lower cavity are separated by a first partition plate; the first partition plate can be pushed into or pulled out from the front side of the preservation device body to realize the separation or penetration between the two cavities; the first partition plate is uniformly provided with exchange holes; the exchange holes are larger than the heads of worker bees and smaller than the thoracic and abdominal parts of worker bees; a drawer is installed at the lower part of the front side of the preservation device body, and the drawer is communicated with the bee isolation room; feeding openings are arranged at the lower parts of the left and right sides of the preservation device body, and the feeding openings are communicated with the bee living room; one feeding opening is communicated with a water feeding part for feeding water to bees; and the other feeding opening is provided with a solid feeding box arranged in the cavity for feeding bees with cube sugar and pollen balls.

[0019] Further, a bee feeding opening extending along the left-right direction is arranged on the top surface of the preservation device body, and the bee feeding opening comprises a sliding opening and a pressing opening on the left and right sides; sliding grooves are arranged on the two inner sides of the sliding opening along the left-right direction, and sliding covers matched with the sliding grooves are inserted into the sliding grooves, and the sliding covers can slide leftward and rightward; the length of the sliding opening is much larger than that of the pressing opening; the sliding opening and the pressing opening are separated by a second partition plate; the pressing opening comprises two parallel arranged blocking sheets, one end of the two blocking sheets is connected by a connecting plate, and the other end can be slidably inserted into the second partition plate; a rectangular pressing frame is fixedly connected to the side of the connecting plate away from the blocking sheets, and a spring is installed at the middle part of the side of the connecting plate close to the blocking sheets.

[0020] Furthermore, the water feeding component includes a water feeding cage, a water storage pipe and a water storage pipe support frame; the water feeding cage includes a support leg and a water feeding cage body in the shape of a rectangular parallelepiped; the water feeding cage body has a water inlet cavity for bees to move, and a plurality of ventilation holes are evenly opened on its left side and bottom surface, and the ventilation holes are connected to the water inlet cavity; the upper side of the water feeding cage body is provided with a square water storage pipe support frame installed on the side of the storage device body; the middle part of the water storage pipe support frame and the top surface of the water feeding cage body are correspondingly provided with water storage pipe installation through holes for installing the water storage pipe ; A top cover is provided at the upper end of the water storage pipe for adding water; there is no cover at the bottom and a water-absorbing sponge is installed, and the water-absorbing sponge can completely block the bottom of the water storage pipe; a lifting block is installed on the side of the water feeding cage body connected to the feeding port, which is used to prevent bees from escaping when replacing the water storage pipe; at the same time, removing the lifting block can realize the connection between the water feeding cage body and the bee living room, which is convenient for the bees to take in water; the solid feeding box can slide left and right along the corresponding feeding port, and the cross-sectional area of ​​the water feeding cage body and the solid feeding box matches the cross-sectional area of ​​the corresponding feeding port.

[0021] Furthermore, a transparent observation window is provided in the middle of the front side of the storage device body, and a plurality of air holes are evenly provided on the transparent observation window; the main ventilation port is in a U-shape and surrounds the transparent observation window.

[0022] Furthermore, a pull-out armrest is provided on a side of the solid feeding box away from the storage device body, and the width of the pull-out armrest is greater than the width of the feeding port.

[0023] Furthermore, the sliding opening is away from the pressing opening at one end, and the upper part of both side surfaces is provided with a limiting groove, and a matching limiting block is inserted in the limiting groove to prevent the sliding cover from accidentally sliding out and causing the bees to escape.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The queen bee preservation method of the present invention establishes a queen bee preservation colony, ensuring that each queen bee has its own accompanying worker bees. This prevents uneven feeding due to varying queen bee abilities, thereby maximizing production and breeding efficiency. Furthermore, the queen bees are preserved in an incubator that simulates the temperature and humidity of a natural bee colony, eliminating the challenges of extreme weather and facilitating long-term queen preservation. The establishment of a queen bee preservation colony and the diverse worker bee replacement options enable the co-preservation of multiple queen bees within the incubator environment.

[0026] 2、The queen bee identity is bound during the preservation process, the queen bee has an identity tag from birth, records the strain, birth date, preservation location and the like, and supports custom record content, can realize account intercommunication between administrators in the system, and reduces the information exchange cost. The queen bee information input and the trend and physiological state of the queen bee can be realized by using the ultra-high frequency RFID inspection equipment, the suitable age queen bee can be found by scanning when the queen bee is preserved in batches in the indoor environment; when applied in the beehive, the queen bee can be found without opening the box, which greatly improves the efficiency of queen bee management. The application can be applied in queen bee artificial breeding and bee farm management, and the whole service process of queen bee application is opened up.

[0027] 3、The queen bee preservation device is specially designed according to the method of the application, the design of the honeybee living room and the honeybee isolation room is considered according to the social habits of the honeybee, so that the honeybees can get familiar with each other in a small environment before they closely contact with the honeybees of different bee colonies, and the behavior of fighting of the worker bees and the behavior of isolating the queen bee due to the error of identity recognition are avoided.

[0028] 4、The feeding method of dry and wet separation can be realized by using the device, which is beneficial to maintaining the healthy preservation state of the honeybee. The traditional method is to feed sugar water and pollen to maintain the living demand of the worker bee, but the sugar water is relatively viscous, and it is easy to adhere to the body of the honeybee after leakage, which leads to poor state and even death, and bacteria are easy to breed. Therefore, the device provides water, cube sugar, pollen and the like for the honeybee, and proposes a feeding method of dry and wet separation, the water feeding part is designed by using the water absorption sponge to avoid the leakage of the stored water; the relatively dry sugar and pollen nutrients and the like are provided separately, and the food state is stable and not easy to deteriorate. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 The queen bee preservation and management method flow chart of the application;

[0030] Figure 2 The queen bee inspection management flow chart of the application;

[0031] Figure 3 The queen bee preservation device structure schematic diagram of the application;

[0032] Figure 4 The queen bee preservation device pressing type opening structure schematic diagram of the application.

[0033] In the figure, 1 - the main body of the storage device, 2 - the honey living room, 3 - the honey isolation room, 4 - the first partition, 5 - the exchange hole, 6 - the push-pull handle, 7 - the main ventilation port, 8 - the transparent observation window, 9 - the air hole, 10 - the auxiliary ventilation port, 11 - the drawer, 12 - the feeding port, 13 - the water feeding part, 14 - the solid feeding box, 15 - the water feeding cage, 16 - the water storage pipe, 17 - the water storage pipe support frame, 18 - the supporting leg, 19 - the water feeding cage main body, 20 - the water absorbing sponge, 21 - the pull-out baffle, 22 - the second partition, 23 - the pull-out handle, 24 - the honeycomb back plate, 25 - the groove, 26 - the sliding opening, 27 - the pressing opening, 28 - the sliding cover, 29 - the sliding handle, 30 - the limiting groove, 31 - the limiting block, 32 - the spring, 33 - the baffle, 34 - the connecting plate, 35 - the rectangular pressing frame. DETAILED DESCRIPTION

[0034] The specific embodiments of the present application will be further described in conjunction with specific examples.

[0035] The numerical ranges in this disclosure are to be understood as specifically encompassing each and every integer within the range. In addition, it is specifically intended that any and all presented ranges be understood to be divided into the smaller ranges that are specifically disclosed, as well as into ranges whose upper and lower limits are essentially the same, but which have different upper and lower limits. Any smaller range that falls within the scope of the larger range is specifically encompassed.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. All documents mentioned herein are incorporated by reference to disclose and describe the methods and / or materials in connection with which the documents are cited. In the case of conflict between the present specification and any document incorporated herein by reference, the present specification will control. Reference to 'comprising', 'containing', 'having', 'including', 'containing', 'including' and the like are to be construed open-ended, i.e. meaning 'comprising but not limited to'.

[0037] The experimental methods used in the present application are conventional methods unless otherwise specified.

[0038] The materials, reagents and the like used in the present application can be purchased or synthesized by known methods unless otherwise specified.

[0039] In the quantitative test in the present application, three repeated experiments are set, and the average value is taken.

[0040] In the following examples, RH refers to relative humidity, expressed in %RH.

[0041] Example 1

[0042] Referring to Figure 1 The embodiment provides a queen bee preservation method, and specifically comprises the following steps.

[0043] S1, a queen bee preservation device is built, which has the function of separating and culturing existing bees and new bees in the case of contacting each other's odor, and can also combine and culture the existing bees and the new bees according to needs. For details, see embodiment 2.

[0044] S2, a queen bee preservation group is formed: according to the length of the preservation time, a certain number of worker bees are selected for queen bee feeding. When it is needed to preserve the queen bee to sexual maturity, about 50 accompanying worker bees can be put in; when it is needed to preserve the queen bee for a long time, about 100 accompanying worker bees can be put in, and the worker bees are replaced once every 30 days according to the death situation. In the selection of worker bees, young worker bees of a queenless group can be selected and put in, at this time, the acceptance ability of the worker bees to the queen bee is higher, and the queen bee time is shorter; if the worker bees of a queen group just out of the house are selected, the worker bees need to be put into the container before the queen bee comes out of the house, so that the worker bees are in a queenless state for a period of time, and are placed in a 35℃ / 60%RH incubator environment for 24h, and then are introduced into the queen stand or the queen bee.

[0045] S3, queen bee identity binding: the queen bee is anesthetized in a 60% CO2 environment for 3 minutes, and after it is still, the identity chip is pasted on the top of the queen bee's dorsal plate with biological glue. Then it is put back into the preservation device, and after about 5 minutes, the queen bee completely recovers the action ability. The queen bee inspection system is opened, the identity chip is scanned, and the strain information and birthday information of the queen bee are recorded to realize the queen bee identity binding.

[0046] S4, feeding worker bees: during the preservation process, pure water needs to be added to the water storage pipe in the preservation device regularly, and the cube sugar and pollen balls in the solid feeding box need to be replaced regularly. Taking 5ml of water as an example, 50 worker bees need to be supplemented with pure water once every 18 hours. Cube sugar and pollen balls are put into the feeding part, and the pollen balls should be replaced every two days in order to maintain freshness.

[0047] S5, environment cleaning and worker bee replacement: after a period of culture, when it is observed that there are dead bees, the bee corpses are cleaned up; when it is needed to supplement new worker bees, the new worker bees are anesthetized and put into the preservation device, so that the new worker bees are separated and cultured from the original bees for 12-20h, and then are combined and cultured; when it is needed to replace the worker bees, the preservation device is anesthetized in 60% CO2, the queen bee and the worker bees are taken out, and then the queen bee and the new worker bees are put into the preservation device for separation and culture, and when the feeding behavior is obvious, the queen bee and the new worker bees are combined and cultured;

[0048] When the device of Example 2 is used for bee preservation, the specific steps for environmental cleaning and worker bee replacement are as follows: After the worker bee dies, it falls onto the first partition of the bee living room. The first partition is pulled out, and the carcass falls into the lower bee isolation chamber. The first partition is pushed in to close the gap, and the drawer in the bee isolation chamber is pulled out to remove the bee carcass and complete the environmental cleaning. When there are more worker bees dying or when the preservation time is longer, young worker bees need to be supplemented or replaced. When supplementing worker bees, the worker bees can be anesthetized with CO2 and placed in the drawer, which is then pushed into the bee isolation chamber. The new and old worker bees can mix their odors at a close distance without fighting. After about 12 hours of preservation in the incubator, the partition can be pulled out to allow the supplemented worker bees to enter the bee living room. When replacing worker bees, the preservation device can be placed in CO2 to anesthetize the original queen bee, which is then placed in the bee isolation chamber in the upper cavity of the bee living room, and new worker bees are placed in the bee living room. The new worker bees can familiarize themselves with the queen bee's odor, and the worker bees' feeding behavior can be observed. After about 24 hours of preservation in the incubator, the partition can be pulled out to allow the queen bee to enter the bee living room.

[0049] S6, Artificial insemination queen supply: At 6 days of age, the queen enters the sexual maturity stage. After the preservation device is anesthetized in CO2 with a concentration of 60% for 3 minutes, the queen is removed and placed on the artificial insemination instrument for operation. The remaining worker bees are placed back into the preservation device, and the worker bees wake up after 5 minutes. After the queen is artificially inseminated, the feeding part can be pulled out, the unconscious queen is placed in it, and then pushed into the bee living room. After the queen wakes up, it can climb out by itself to avoid mechanical damage caused by shaking the device. Then, the preservation device is placed back in the incubator.

[0050] S7, Queen inspection management: The queen is bound to an identity tag after birth, so when feeding or checking the queen in the preservation device, the queen information in the current device can be obtained by scanning with the inspection equipment. Through this operation, the current queen can be quickly inquired about which category it belongs to, and the corresponding high-quality drone category can be prepared for crossbreeding. The record of the time out of the room can quickly find the queen in the appropriate insemination period. After the queen is inseminated, it needs to be returned to the beehive for production and application. The preservation device number can be changed to the corresponding beehive number. When the queen is preserved in the beehive, the queen's movement can be continuously monitored without opening the beehive through the mutual induction of the inspection equipment and the identity chip, realizing the whole process management of the preserved queen.

[0051] Reference Figure 2, in order to facilitate the management of queen bee in the queen bee preservation process, the queen bee inspection management and preservation steps are also designed in the application. Specifically, after the queen bee is born, its identity is bound. The preservation device containing the queen bee is placed in a CO2 environment with a concentration of 60% to anesthetize the queen bee for 3 minutes, and after it is still, the identity chip is pasted on the top of the queen bee back plate with biological glue. Then it is put back into the preservation device, and after about 5 minutes, the queen bee fully recovers the action ability. Open the queen bee inspection system, scan the identity chip, and record its strain information and birthday information to realize the queen bee identity binding. When feeding or checking the queen bee in the preservation device, the queen bee information in the current device can be obtained by scanning with the inspection equipment. Through this operation, the current queen bee can be quickly inquired, which is convenient for preparing high-quality drones for crossbreeding; at the same time, the record of the time of leaving the room can help to quickly find the queen bee in the appropriate insemination period. After the queen bee insemination is completed, it needs to be put back into the beehive for production and application, the preservation device number can be changed to the corresponding beehive number, and when the queen bee is preserved in the beehive, the movement of the queen bee can be continuously monitored without opening the beehive through the mutual induction of the inspection equipment and the identity chip, realizing the whole process management of the preserved queen bee. If the queen bee is not read in the first inspection, the second inspection needs to be carried out, and if the queen bee still cannot be read, the beehive needs to be opened for inspection to check whether the queen bee is lost, and the queen bee state can be preserved or changed after the situation is identified. Finally, the operation log is generated according to the operation record, and the inspection is completed. The automatic operation log function makes the queen bee management more efficient and detailed.

[0052] In the queen bee preservation method of the application, the establishment of the queen bee preservation colony and the diversified worker bee replacement scheme can realize the common preservation of multiple queen bees in an incubator environment. The establishment of the queen bee preservation colony allows each queen bee to have its own accompanying worker bee, avoiding uneven feeding caused by uneven queen bee ability, and maximizing the production and breeding efficiency. Preservation in an incubator that simulates the temperature and humidity of a natural beehive will not be challenged by extreme weather, which is conducive to long-term preservation of queen bees. At the same time, the binding of the queen bee identity enables the queen bee category, physiological state and movement to be known without opening the beehive, greatly facilitating the whole process management of the queen bee.

[0053] Example 2

[0054] As Figure 3As shown, the present invention also provides a queen bee preservation device for implementing the above-mentioned queen bee preservation method. The device comprises a rectangular preservation device body 1, which contains a cavity for bee activities, including an upper and lower cavity. The upper cavity is a bee living chamber 2, and the lower cavity is a bee isolation chamber 3. The upper and lower cavities are separated by a first partition 4. The first partition 4 can be pushed in or out from the front side of the preservation device body 1 to separate or connect the two cavities. The first partition 4 is evenly distributed with communication holes 5. The communication holes 5 are larger than the head of the worker bees and smaller than the chest and abdomen of the worker bees. The design of the bee living chamber 2 and the bee isolation chamber 3 fully considers the social habits of bees. Before bees from different bee colonies come into close contact, they are allowed to familiarize themselves with each other's scent in a small environment, thereby avoiding fighting between worker bees or even isolating the queen bee due to identity errors. A push-pull handle 6 is fixedly connected to the end of the first partition 4 away from the preservation device body 1 to facilitate the withdrawal and insertion of the first partition 4. In this embodiment, the storage device body 1 measures 10 cm in length, 4 cm in width, and 12 cm in height, respectively. A drawer 11 is mounted on the lower front side of the storage device body 1. This drawer 11 communicates with the honey-sealed isolation chamber. The front side of this drawer 11 is made of transparent material. This facilitates the removal of dead bees and also serves as a bee entry point. The transparent front side of this drawer 11 facilitates observation of the bees.

[0055] The front and rear sides of the storage device body 1 are provided with several strip-shaped main vents 7, which communicate with the cavity of the storage device body 1. A transparent observation window 8 is located in the center of the front side of the storage device body 1, and a number of air holes 9 are evenly distributed in the transparent observation window 8. The main vents 7 surround the transparent observation window 8 in a U-shaped pattern. Several strip-shaped secondary vents 10 are evenly and symmetrically located in the upper and middle portions of the left and right sides of the storage device body 1, which communicate with the bee living chamber 2. The multiple vents on all four sides of the storage device body 1 and the transparent observation window 8 ensure both ventilation and convenient observation of the bees.

[0056] The left and right side lower part of the storage device body 1 is provided with a feeding port 12, which is communicated with the bee living room 2; one feeding port 12 is communicated with the water feeding part 13 for feeding water to the bees; the other feeding port 12 is provided with a solid feeding box 14 in the cavity for feeding bees with cube sugar and pollen balls. The water feeding part 13 comprises a water feeding cage 15, a water storage pipe 16 and a water storage pipe support frame 17; the water feeding cage 15 comprises a supporting leg 18 and a cuboid-shaped water feeding cage body 19; the water feeding cage body 19 has a water inlet cavity for the bees to move, and a plurality of ventilation holes are uniformly arranged on the left side and the bottom surface of the water feeding cage body 19; the ventilation holes are communicated with the water inlet cavity; the upper side of the water feeding cage body 19 is provided with a square water storage pipe support frame 17 mounted on the side of the storage device body 1; the middle part of the water storage pipe support frame 17 and the top surface of the water feeding cage body 19 are provided with a water storage pipe mounting hole for mounting the water storage pipe 16; the upper end of the water storage pipe 16 is provided with a top cover for adding water; the bottom is not covered and is provided with a water absorbing sponge 20, which can completely block the bottom of the water storage pipe 16; the side of the water feeding cage body 19 communicated with the feeding port 12 is provided with a pull-off baffle 21 for avoiding the bees escaping when replacing the water storage pipe 16; at the same time, removing the pull-off baffle 21 can realize the communication between the water feeding cage body 18 and the bee living room 2, which is convenient for the bees to drink water; the solid feeding box 14 can slide left and right along the corresponding feeding port 12, and the side of the solid feeding box 14 away from the storage device body 1 is provided with a pull-out handle 23 for conveniently pulling out and pushing in the solid feeding box 14, and the width of the pull-out handle 23 is greater than the width of the feeding port 12 to prevent the pushing force from being too large, which causes the solid feeding box 14 to be unable to be pulled out. The cross-sectional area of the water feeding cage body 19 and the solid feeding box 14 matches the cross-sectional area of the corresponding feeding port 12. In this embodiment, the feeding port 12 connected with the water feeding cage 15 is 2.5 cm long and 1.5 cm wide; the total height of the water feeding cage 15 is about 3.5 cm, the height of the supporting leg 18 is about 1.5 cm, the length of the water feeding cage 15 body is about 3.5 cm, the width is about 2.5 cm, and the height is about 2 cm; the feeding port 12 provided with the solid feeding box 14 is 3.0 cm long and 1.5 cm wide; the solid feeding box 14 is 4 cm long, 3 cm wide and 1.5 cm high. When feeding bees, the traditional method is to feed sugar water and pollen to maintain the life needs of worker bees, but the sugar water is relatively viscous and easy to stick to the bees after leaking, which causes the bees to be in poor condition or even die, and bacteria are easy to breed. Therefore, this scheme provides water, cube sugar, pollen and other resources for bees, proposes a dry and wet separation feeding method, and designs a water feeding part 13 with a water absorbing sponge 20 to avoid water leakage; separate dry sugar and pollen nutrients to maintain the stable state of the food and the healthy storage state of the bees.

[0057] The top surface of the storage device body 1 is provided with a honeycomb backboard 24 insertion port and a bee feeding port extending along the left-right direction, both of which are in communication with the cavity; and the bee feeding port is arranged at one end close to the front side of the storage device body 1; and the honeycomb backboard 24 insertion port is arranged at one end close to the back side of the storage device body 1.

[0058] The honeycomb backboard 24 insertion port is slidably inserted with a honeycomb backboard 24, which is plate-shaped and has a cross-sectional area matched with the honeycomb backboard 24 insertion port to achieve a sealing effect; the lower end of the honeycomb backboard 24 abuts against the bottom of the cavity, and the upper end of the honeycomb backboard 24 is located outside the storage device body 1 and is provided with a groove 25 extending along the left-right direction, facilitating the insertion and extraction of the honeycomb backboard 24 for regular cleaning; the part of the honeycomb backboard 24 located in the cavity is provided with a plurality of hexagonal biomimetic nest holes; simulating a real nest, bees can climb and adhere to the biomimetic nest holes, effectively avoiding the aggregation of bees at the bottom of the cavity, providing a better living environment for bees, reducing the difficulty of culturing bees in groups, and improving the experimental efficiency and success rate.

[0059] The bee feeding port includes left and right sliding openings 26 and a pressing opening 27; the two inner sides of the sliding opening 26 are both provided with a sliding groove extending along the left-right direction, and a sliding cover 28 matched with the sliding groove is inserted in the sliding groove; the sliding cover 28 can slide left and right to control the size of the sliding opening 26; the sliding opening 26 has a large opening to facilitate the batch loading of anesthetized worker bees. The top of the sliding cover 28 is provided with a sliding handle 29 to facilitate the sliding in and out of the sliding cover 28. The end of the sliding opening 26 away from the pressing opening 27 is provided with a limiting groove 30 on the upper part of the two sides, and a limiting block 31 matched with the limiting groove 30 is inserted in the limiting groove 30 to prevent the sliding cover 28 from sliding out by mistake and causing the bees to escape.

[0060] In combination Figure 4 It can be seen that the sliding opening 26 and the pressing opening 27 are separated by the second partition plate 22; the pressing opening 27 includes two parallel arranged flaps 33, one end of the two flaps is connected by a connecting plate 34, and the other end can be slidably inserted into the second partition plate 22; the side of the connecting plate 34 away from the flaps 33 is fixedly connected with a rectangular pressing frame 35, and the middle part of the side of the connecting plate 34 close to the flaps 33 is installed with a spring 32. In use, by pressing the rectangular pressing frame 35, the flaps 33 move towards the second partition plate 22 and are inserted into the second partition plate 22; at this time, the opening of the rectangular pressing frame 35 is in communication with the cavity, and bees can be fed into the storage device through the opening; when the rectangular pressing frame 35 is released, the spring 32 will automatically rebound, and the feeding port is closed. The automatic rebound design can avoid the escape of bees in the device when it is necessary to load active bees.

[0061] The honeycomb backboard 24 in the embodiment is 9 cm long, 0.5 cm wide and 9 cm high; the sliding opening 26 is 8 cm long and 1.5 cm wide; the pressing opening 27 is 1.5 cm long and 1 cm wide; and the sliding cover 28 is 8 cm long, 2 cm wide and 7 cm high.

[0062] Working principle: when a batch of bees needs to be put in, the limiting block 31 on one side of the sliding opening 26 is removed, the sliding cover 28 is slid to open the sliding opening 26, the bees are put into the cavity of the storage device main body 1, the sliding cover 28 is slid again to close the sliding opening 26, and the limiting block 31 is inserted into the limiting groove 30 to prevent the sliding cover 28 from being misoperated and causing the bees to escape. (When a small amount of bees needs to be put in, the pressing opening 27 is opened for putting in.) Then the pull-off baffle 21 is put down, the water storage pipe is taken out, the top cover is removed, pure water is injected into the water storage pipe, the water storage pipe is sequentially inserted into the water storage pipe support frame and the water feeding cage 15, then the pull-off baffle 21 is pulled up, the water feeding cage 15 and the bee living room 2 are connected, the bees are facilitated to drink water. At the same time, the solid feeding box 14 is pulled out, the square sugar and pollen balls are put in, then the solid feeding box 14 is pushed into the bee living room 2, and the bees are facilitated to eat. The adding frequency of the pure water is that, for example, 5 ml of water, 50 worker bees need to be supplemented with pure water once about every 18 hours; and the square sugar and pollen balls are replaced every two days to ensure freshness. Finally, the storage device main body 1 is placed in a culture box at 35 DEG C / 60% RH to store, so that the real living conditions of the bees are restored as much as possible, the survival rate of the bees is improved, extreme weather is avoided, and the bees are beneficial to be stored for a long time. When the bees need to be taken, the storage device main body 1 is usually placed in 60% CO2 for 3 minutes of anesthesia, then the first partition plate 4 is taken out, and the bees falling into the drawer 11 and the solid feeding box 14 are taken out.

[0063] It can be known that the queen bee storage device has reasonable design of each component and strong functionality. The four-side ventilation design is beneficial to ventilation of the stored bee colony, the front side is provided with a ventilation observation window, biological observation is facilitated, the design of the bee living room and the bee isolation room fully considers the social habits of the bees, the bees are familiar with each other in a small environment before closely contacting different bee colonies, and the behavior of fighting of worker bees or isolation of a queen bee due to identity recognition error is avoided. The water feeding component with the isolation function in the storage device can conveniently supplement drinking water of the bees and avoid escape of the bees.

[0064] In addition, the application adopts a dry-wet separation feeding method, which is beneficial to maintaining the healthy state of the bees. In the traditional method, sugar water and pollen are fed to meet the living needs of the bees, but the sugar water is relatively thick, and after leaking, it is easy to adhere to the bees, causing the bees to be in poor condition or even die, and bacteria are easy to breed. Therefore, the application provides water, granulated sugar, pollen and other resources for the bees, and proposes a dry-wet separation feeding method, and the water feeding component is designed to use a water-absorbing sponge to avoid the leakage of stored water; relatively dry sugar and pollen nutrients and other substances are provided separately, and the food state is stable and not easy to deteriorate.

[0065] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the technical solutions. Those of ordinary skill in the art should understand that modifications or equivalent replacements to the technical solutions of the present application without departing from the purpose and scope of the technical solutions should be covered in the scope of the claims of the present application.

Claims

1. A queen bee preservation device, comprising a preservation device body, a cavity for bees to move in the preservation device body, a plurality of strip-shaped main ventilation openings on the front and back sides of the preservation device body, the main ventilation openings being in communication with the cavity of the preservation device body; characterized in that, The cavity comprises two cavities, an upper cavity and an lower cavity; wherein the upper cavity is a bee living chamber, and the lower cavity is a bee isolation chamber; the upper cavity and the lower cavity are separated by a first partition; the first partition can be pushed in or pulled out from the front side of the storage device body to achieve separation or communication between the two cavities; communication holes are evenly opened on the first partition; the communication holes are larger than the head of the worker bee and smaller than the chest and abdomen of the worker bee; a drawer is installed at the lower part of the front side of the storage device body, and the drawer is connected to the honey-sealed isolation chamber; feeding ports are opened at the lower parts of the left and right sides of the storage device body, and the feeding ports are connected to the bee living chamber; one feeding port is connected to the water feeding component for feeding water to the bees; the other feeding port is installed with a solid feeding box located in the cavity, for feeding sugar cubes and pollen balls to the bees; The top surface of the storage device body is provided with a bee insertion opening extending in the left and right directions, and the bee insertion opening includes a sliding opening and a press-type opening on the left and right sides; a slide groove is penetrated through the two inner side surfaces of the sliding opening in the left and right directions, and a sliding cover matching the slide groove is inserted in the slide groove, and the slide cover can slide left and right; and the length of the sliding opening is much greater than that of the press-type opening; the sliding opening and the press-type opening are separated by a second partition; the press-type opening includes two parallel blocking pieces, one end of the two blocking pieces is connected by a connecting plate, and the other end can be slidably inserted into the second partition; a rectangular pressing frame is fixedly connected to the side of the connecting plate away from the blocking piece, and a spring is installed in the middle of the side of the connecting plate close to the blocking piece; The water feeding component includes a water feeding cage, a water storage pipe and a water storage pipe support frame; the water feeding cage includes a support leg and a water feeding cage body in the shape of a rectangular parallelepiped; the water feeding cage body has a water inlet cavity for bees to move, and a plurality of ventilation holes are evenly opened on the left side and bottom surface thereof, and the ventilation holes are connected to the water inlet cavity; the upper side of the water feeding cage body is provided with a square water storage pipe support frame installed on the side surface of the storage device body; the middle part of the water storage pipe support frame and the top surface of the water feeding cage body are correspondingly provided with water storage pipe installation through holes for installing the water storage pipe; The upper end of the water storage pipe is provided with a top cover for adding water; the bottom is uncovered and is equipped with a water-absorbing sponge, which can completely block the bottom of the water storage pipe; a lifting baffle is installed on the side of the water feeding cage body connected to the feeding port, which is used to prevent bees from escaping when replacing the water storage pipe; at the same time, removing the lifting baffle can realize the connection between the water feeding cage body and the bee living room, which is convenient for the bees to enter water; the solid feeding box can slide left and right along the corresponding feeding port, and the cross-sectional area of ​​the water feeding cage body and the solid feeding box matches the cross-sectional area of ​​the corresponding feeding port.

2. The queen bee preservation device of claim 1, wherein, A transparent observation window is provided in the middle of the front side of the storage device body, and a plurality of air holes are evenly arranged on the transparent observation window; the main ventilation port is in a U-shaped shape and surrounds the transparent observation window.

3. The queen bee preservation device of claim 1, wherein, A pull-out handrail is provided on a side of the solid feeding box away from the storage device body, and the width of the pull-out handrail is greater than the width of the feeding port.

4. The queen bee preservation device of claim 1, wherein, The sliding opening is away from one end of the pressing opening, upper portions of two side faces are each provided with a limiting slot, a limiting block matched with the limiting slot is inserted into the limiting slot, and the limiting block is used for preventing the sliding cover from being mis-slid to cause the bees to escape.

5. A method for preserving queen bees based on the device according to any one of claims 1 to 4, characterized in that, It comprises the following steps: (1) Assembling queen bee preservation group: assemble the queen bee preservation group according to the quantity ratio of queen bee to worker bee being 1:50-100, and place the queen bee and the worker bee into a queen bee preservation device for preservation, and then place the preservation device into a culture box with a temperature of 35 DEG C and a relative humidity of 60%; the queen bee preservation device has the function of separating the culture of the existing bees and the new bees under the condition of contacting each other's odor, and can also combine the culture of the existing bees and the new bees according to the need; (2) Bee feeding: during the preservation process, it is necessary to add pure water to the water storage pipe in the preservation device at regular intervals, and replace the cube sugar and pollen balls in the solid feeding box at regular intervals; (3) Bee cleaning and worker bee replacement: after a period of culture, when it is observed that there are dead bees, the dead bees are cleaned up; when it is necessary to supplement new worker bees, the new worker bees are placed into the preservation device after being anesthetized, so that the new worker bees are cultured separately from the existing bees under the condition of contacting each other's odor for 12-20 hours, and then the new worker bees are combined with the existing bees for culture; when it is necessary to replace the worker bees, the preservation device is placed into CO2 with a concentration of 60% for anesthetization, the queen bee and the worker bees are taken out, the queen bee and new worker bees are placed into the preservation device for separate culture, and when obvious feeding behavior appears, the queen bee and the new worker bees are combined for culture; (4) Artificial insemination of queen bee: when the queen bee enters the sexual maturity stage, the preservation device is placed into CO2 with a concentration of 60% for anesthetization, the bees are taken out, artificial insemination is performed on the queen bee, and the remaining worker bees are placed back into the preservation device; after the queen bee is inseminated, the queen bee is placed back into the preservation device, and then the preservation device is placed back into the culture box for preservation.

6. The queen bee preservation method of claim 5, wherein, In the step (1) of assembling queen bee preservation group, when it is necessary to preserve the queen bee to sexual maturity, one queen bee can be placed into 50 accompanying worker bees; when it is necessary to preserve the queen bee for a long time, 100 accompanying worker bees are placed, and the worker bees are replaced once every 30 days according to the death condition; in the selection of worker bees, young worker bees of a queenless group are selected and placed, at this time, the worker bees have high acceptance ability to the queen bee, and the queenless time is short; if worker bees of a group with a queen are selected, the worker bees are placed into the container before the queen bee leaves the house, so that the worker bees are in a queenless state for a period of time, and are placed in a culture box with a temperature of 35 DEG C and a relative humidity of 60% for preservation for 24 hours, and then the worker bees are introduced into the queen stand or the queen bee.

7. The queen bee preservation method of claim 5, wherein, In the step (2) of bee feeding, the adding frequency of the pure water is that, taking 5ml of water as an example, 50 worker bees need to be supplemented with pure water once every 18 hours; the cube sugar and the pollen balls are replaced once every two days to ensure freshness.

8. The queen bee preservation method of claim 5, wherein, It also comprises queen bee inspection management: before the step (2), the preservation device is placed into CO2 with a concentration of 60% for anesthetization for 3 minutes, the queen bee is taken out, an identity chip is pasted on the back of the queen bee, and then the queen bee is placed back into the preservation device, a queen bee inspection system is turned on, the identity chip is scanned, and the strain information and the birthday information of the queen bee are recorded, so that the queen bee identity binding can be realized. During the storage process, the inspection device is used to perform a surrounding scan on the storage device to monitor the physiological state and movement of the queen bee, so as to facilitate management of the queen bee.

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