Erwinia persicina 2022 tibbst187 and application thereof

Abstract

The application discloses an Erwinia persicina 2022TIBBST187 strain, which is classified and named as Erwinia persicina, is preserved in the China Center for Type Culture Collection, has a preservation number of CCTCC NO: M 2022872, is preserved on June 13, 2022, and is located at Wuhan University, Wuhan, China. The application also discloses application of the Erwinia persicina 2022TIBBST187 strain in biological control, plant growth promotion and stress resistance. The BST187 strain has the advantages of salt tolerance, efficient organic phosphorus degradation and the like, provides a good biocontrol resource for prevention and treatment of bacterial diseases of animals and plants, and provides an active ingredient with potential value for research and development of biological pesticides, i.e., andrimid, and has important application value in the field of biological control.

Description

Technical Field

[0001] This invention belongs to the fields of microbiology, biotechnology and biological control technology, and relates to a strain of Erwinia peachii 2022TIBBST187 and its application. Background Technology

[0002] Biological control, adhering to the principle of sustainable agricultural development, utilizes beneficial organisms or their secondary metabolites to inhibit or mitigate the occurrence and development of harmful organisms. This method not only specifically controls pests and diseases but also boasts advantages such as being environmentally friendly and leaving no chemical residues, achieving the dual benefits of ecological environmental protection and increased agricultural production. As a new industry in the 21st-century pesticide industry, biopesticides are a major means of biological control, representing a new direction in plant protection. Their advantages of no environmental pollution and no pesticide residues greatly improve the quality and price of agricultural products. Therefore, the research and application of biopesticides have an immeasurable driving effect on vigorously promoting rural economic growth and increasing farmers' income.

[0003] The foundation of biological control lies in functional organisms with biocontrol effects, among which biocontrol functional microorganisms have been extensively studied. The number of microbial fertilizers registered with biocontrol functional microorganisms as active ingredients peaked in 2018, reaching 3,800 varieties. In recent years, progress in discovering new microbial strains has been relatively slow, with registered strains still predominantly Bacillus, Trichoderma, and yeast. However, a few new functional microorganisms have been discovered, including Burkholderia, Pantotheca acuminata, and Burkholderia. Therefore, continuous discovery and screening of new biocontrol and growth-promoting functional strains, and expanding the resource bank of functional microbial strains, is essential.

[0004] Currently, the main technology for biological control still relies on the direct application of bacterial strains or inoculants to control diseases. Therefore, the shelf life and efficacy stability of these products are difficult to guarantee. Only by extracting and purifying their antimicrobial substances (antibiotics and antimicrobial proteins) can microbial fermentation products be transformed into truly standardized biological pesticides, thus enabling their better application in agricultural production. However, approximately 90% of microbial natural products and over 99% of microbial secondary metabolites remain to be discovered. Furthermore, research indicates that these bioactive natural products possess diverse ecological functions, including directly acting on pathogens to protect hosts from infection, inducing host resistance to enhance disease resistance, and altering environmental adaptability. Summary of the Invention

[0005] One object of the present invention is to solve at least the above-mentioned problems and / or defects, and to provide at least the advantages described below.

[0006] Another object of the present invention is to provide a strain of Erwinia peachensis 2022TIBBST187.

[0007] Another objective of this invention is to provide a method for producing Erwinia pinka fermentation broth and the active substance andrimid.

[0008] Another objective of this invention is to provide the application of Erwinia peachii strain 2022TIBBST187 and the active substance andrimid.

[0009] Therefore, the technical solution provided by this invention is as follows:

[0010] Erwinia persicina strain 2022TIBBST187, classified as Erwinia persicina, is deposited at the China Center for Type Culture Collection (CCTCC) on June 13, 2022, with accession number CCTCC NO: M 2022872. The deposit date is Wuhan University, Wuhan, China.

[0011] The production method of Erwinia pink, namely the production method of Erwinia pink fermentation broth and active substances, includes the following steps:

[0012] The Erwinia persicina 2022TIBBST187 strain was inoculated into LB liquid medium and cultured to obtain fermentation broth.

[0013] Preferably, the production method further includes the following steps: removing the bacterial cells from the fermentation broth to obtain a supernatant, adding ammonium sulfate to the supernatant to a final concentration of 80% (w / v) and letting it sit overnight, then collecting the precipitate, adding methanol to dissolve the precipitate to a concentration of 0.1 g / mL, to obtain a crude extract of andrimid.

[0014] Preferably, in the production method, the culture is carried out at a temperature of 28°C and a rotation speed of 200 rpm for 24 hours.

[0015] The application of Erwinia philippinii strain 2022TIBBST187 in biological control, promoting plant growth and stress resistance, including its application in the preparation of biocontrol agents.

[0016] Preferably, in the aforementioned application, the Erwinia rosenbergii 2022TIBBST187 strain inhibits the growth of bacterial pathogens.

[0017] Preferably, the application includes the production of andrimid.

[0018] Preferably, in the aforementioned application, the angeminin inhibits the growth of bacterial pathogens.

[0019] Preferably, in the application, the application includes Erwinia lavophila 2022TIBBST187 strain or angemicatin to inhibit the growth of the following pathogens: Rhizoctonia solani, Ralstonia solanacearum, and Dichotomyces solani.

[0020] Preferably, in the application, the *Erwinia pink* 2022TIBBST187 strain exhibits salt tolerance and is capable of solubilizing organophosphates. Strain BST187 can grow normally in LB medium with a salt concentration of 10% or lower, even if the medium becomes turbid, and can be used to promote plant growth in saline soils. Strain BST187 has a strong ability to solubilize organophosphates, with a phosphorus solubilization index as high as 4.26 on day 7, and can be used to promote the dissolution of organophosphates in the soil.

[0021] The present invention has at least the following beneficial effects:

[0022] This invention provides a multifunctional Erwinia purpurea strain 2022TIBBST187 and its secretion angeminin, as well as their applications in biocontrol and growth promotion. This strain was deposited at the China Center for Type Culture Collection (CCTCC) on June 13, 2022, with the sample name Erwinia purpurea 2022TIBBST187 and accession number CCTCC M2022872. This strain 2022TIBBST187 (hereinafter referred to as BST187) can effectively inhibit the growth of bacterial pathogens such as *Ralstonia solanacearum* (the pathogenic form of pepper spot disease), *Ralstonia solanacearum*, and *Dicotyle solanum*. In addition to its antibacterial effect, strain BST187 also exhibits salt tolerance and efficient organophosphate solubilization, promoting growth and stress resistance. Furthermore, angeminin was isolated from Erwinia purpurea for the first time and possesses highly effective antibacterial properties. Therefore, strain BST187 not only provides a good biocontrol resource for the prevention and control of bacterial diseases in plants and animals, but its produced angimycin also provides a potentially valuable active ingredient for the research and development of biological pesticides, and has important application value in the field of biological control.

[0023] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0024] Figure 1 This is a plate diagram of the initial screening of organophosphate strains in an embodiment of the present invention. The arrow points to strain BST187.

[0025] Figure 2This is a graph showing the phosphorus dissolution effect of BST187 on day 7 in an embodiment of the present invention - the plate phosphorus dissolution ring method.

[0026] Figure 3 This is a graph showing the change in phosphorus solubility of BST187 over time in an embodiment of the present invention - liquid culture method.

[0027] Figure 4 This is a graph showing the pH change of the culture medium during the phosphorus dissolution process of BST187 in an embodiment of the present invention - liquid culture method.

[0028] Figure 5 This is a plate antagonism test diagram of the antibacterial effect of BST187 bacteria in an embodiment of the present invention.

[0029] Figure 6 This is a graph showing the comparative analysis results of the antibacterial effects of BST187, biocontrol strains QST713 and BST23 on Xcv in the embodiments of the present invention.

[0030] Figure 7 This is a graph showing the comparative analysis results of the antibacterial effects of BST187, biocontrol strains QST713 and BST23 on Ds in the embodiments of the present invention.

[0031] Figure 8 This is a graph showing the comparative analysis results of the antibacterial effects of BST187, biocontrol strains QST713 and BST23 on Rs in the embodiments of the present invention.

[0032] Figure 9 This figure shows the antibacterial effect of the crude extract of angiin, the active substance obtained after ammonium sulfate precipitation of the metabolites from solid-state and liquid fermentation of strain BST187 in this invention. Xcv: *Rhizoctonia solani*, the pathogenic form of pepper spot disease; DS: *Dichotomum solanum*; RS: *Rhizoctonia solani*.

[0033] Figure 10 This is a diagram showing the analysis results of the Antismash gene cluster, a secondary metabolite of strain BST187 in this embodiment of the invention.

[0034] Figure 11 This is a liquid chromatogram of the crude extract of angiin in an embodiment of the present invention.

[0035] Figure 12 These are the primary and secondary mass spectra of angiotensin in this embodiment of the invention. Detailed Implementation

[0036] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.

[0037] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not imply the presence or addition of one or more other elements or combinations thereof.

[0038] It should be noted that, unless otherwise specified, the experimental methods described in the following implementation plan are all conventional methods, and the reagents and materials described are all commercially available unless otherwise specified.

[0039] To enable those skilled in the art to better understand the technical solution of the present invention, the following embodiments are provided for illustration: The strains and their sources involved in the following examples are as follows:

[0040] Table 1. Bacterial pathogens and their sources

[0041]

[0042] Example 1: Isolation and screening of strain BST187

[0043] Tomato rhizosphere soil was collected from Dongli District, Tianjin in 2022. 1g of soil was mixed with 99mL of 1×PBS buffer (dilution factor 10). 2 Shake at 200 rpm and 28℃ for 30 min. After standing for 20 min, take the supernatant of the soil suspension for serial dilution, and take samples at each dilution factor of 10. 2 10 3 10 4 100 μL of the diluted solution was evenly spread on inorganic phosphate-solubilizing medium (10 g / L glucose, 0.5 g / L ammonium sulfate, 0.5 g / L yeast extract, 0.3 g / L sodium chloride, 0.3 g / L potassium chloride, 0.3 g / L magnesium sulfate, 0.03 g / L ferrous sulfate, 0.023 g / L manganese sulfate, 5 g / L calcium phosphate, and 15 g / L agar) and organic phosphate-solubilizing medium (the calcium phosphate in the inorganic phosphate-solubilizing medium was replaced with 0.2 g / L lecithin and 1 g / L calcium carbonate, with the other components remaining the same). The plates were then incubated upside down at 28°C for 24-48 hours. The formation of phosphate-solubilizing zones could be observed on the phosphate-solubilizing medium, allowing for preliminary screening of phosphate-solubilizing bacteria.

[0044] For the purification of single colonies, select colonies that produce phosphate-solubilizing zones. First, use a sterile inoculation loop to pick bacterial colonies and streak them on LB solid medium plates for purification until the colony morphology is uniform. Then, propagate the single colonies in LB liquid medium. Mix the bacterial suspension with sterile glycerol (working concentration 25%) and store it in a -80°C refrigerator for later use.

[0045] Through the above-described process of isolating and screening phosphate-solubilizing bacteria, we isolated several strains with phosphate-solubilizing zones from organic phosphorus-solubilizing culture media, as shown in the following results. Figure 1As shown. This invention mainly targets one of the phosphate-solubilizing bacteria, which is named 2022TIBBST187 (hereinafter referred to as BST187).

[0046] Erwinia persicina strain 2022TIBBST187, taxonomically named *Erwinia persicina*, is deposited at the China Center for Type Culture Collection (CCTCC) on June 13, 2022, with accession number CCTCC NO: M 2022872. The deposit date is June 13, 2022, and the address of the institution is Wuhan University, Wuhan, China.

[0047] Example 2: Molecular biological identification of strain BST187

[0048] This example demonstrates the molecular biological identification of the BST187 monoclonal strain by sequencing its 16S ribosomal RNA (16S rRNA). Specifically, the 16S rDNA sequence was amplified using the universal bacterial primer pair 27F / 1492R. The PCR amplification reaction volume of 25 μL is shown in Table 2. The PCR reaction conditions were: 94℃ pre-denaturation for 5 min; 94℃ denaturation for 30 s, 56℃ annealing for 30 s, 72℃ extension for 1 min, 30 cycles; 72℃ extension for 5 min.

[0049] Whole-genome sequencing analysis was performed on strain BST187. The BST187 bacterial suspension was sent to Guangdong Megagene Technology Co., Ltd. for sequencing and construction of a detailed bacterial map. The main workflow included three parts: DNA extraction and library construction, high-throughput sequencing (Illumina and Nanopore), and genome assembly and subsequent analysis. The full-length BST187 genome is 4.85 Mbp and contains 4662 genes. The average nucleotide similarity (ANI) of the assembled BST187 genome was 99% similar to that of Erwinia peach Cp2.

[0050] The assembled bacterial framework was further analyzed using average nucleotide similarity (ANI) to identify the genomic relationships of the bacteria. An ANI > 95% indicated that the two genomes belonged to the same species. Table 3 shows the ANI analysis results of Erwinia peache Cp2 from the reference group.

[0051] Table 2 Polymerase Chain Reaction System

[0052] Components Volume (μL) 2×TaqMix 12.5 Forward primer (10 pmol) 1 Reverse primer (10 pmol) 1 DNA model 1 <![CDATA[ddH2O]]> 9.5

[0053] Table 3. Results of average nucleotide similarity analysis

[0054]

[0055]

[0056] The amplified product was detected by agarose gel electrophoresis and then sent to Beijing Qingke Biotechnology Co., Ltd. for sequencing. The obtained sequence is shown in SEQ ID NO:1. The sequencing results were analyzed using SnapgeneViewer and MEGA11. At the same time, BLAST comparison was performed on the NCBI website to determine the strain classification. The results showed that strain BST187 had a similarity of up to 99% with Erwinia persicina Cp1.

[0057] Based on the morphological characteristics of the bacteria and the results of average nucleotide similarity analysis, strain BST187 was identified as Erwinia persicina.

[0058] Example 3: Verification of the phosphorus solubilization function of strain BST187

[0059] 3.1 Determination of the phosphorus solubilization activity of BST187 using the plate phosphorus solubilization ring method

[0060] The culture medium required for the phosphorus solubilization activity assay is: glucose 10 g / L, ammonium sulfate 0.5 g / L, yeast extract 0.5 g / L, sodium chloride 0.3 g / L, potassium chloride 0.3 g / L, magnesium sulfate 0.3 g / L, ferrous sulfate 0.03 g / L, manganese sulfate 0.023 g / L, calcium phosphate 5 g / L (organic phosphorus replaced by: lecithin 0.2 g / L, calcium carbonate 1 g / L) and agar 15 g / L.

[0061] The preserved bacterial strain was removed from the -80℃ freezer and activated by streaking on LB solid medium. After incubating upside down at 28℃ for 1 day, a single colony was picked and inoculated into LB liquid medium, and incubated overnight at 28℃ and 200 rpm. 2 μL of the bacterial suspension was then agarically planted in the center of both organic and inorganic phosphorus solid media. After the bacterial suspension was dried, it was incubated upside down at 28℃. The diameter of the phosphorus-solubilizing zone (D) and the colony diameter (d) were measured and recorded every 24 hours after the appearance of the phosphorus-solubilizing zone. The phosphorus-solubilization index (PSI) was calculated to assess the phosphorus-solubilizing ability of the strain; the formula is PSI = D / d.

[0062] The results are as follows Figure 2 As shown in Table 4, strain BST187 has a strong ability to solubilize organic phosphorus, with a phosphorus solubility index as high as 4.26 on day 7, but a weak ability to solubilize inorganic phosphorus.

[0063] Table 4. Changes in the organic phosphorus index of BST187 over 7 days.

[0064] Measurement time Phosphorus Solubility Index (PSI) Day 2 2.51 Day 3 2.98 Day 4 3.17 Day 5 3.01 Day 6 3.40 Day 7 4.26

[0065] 3.2 Quantitative determination of phosphorus solubilization capacity of BST187 by liquid culture method

[0066] The preserved bacterial strain was removed from the -80℃ freezer and activated by streaking on LB solid medium. After incubation at 28℃ for 1 day (inverted), a single colony was picked and inoculated into LB liquid medium, and incubated overnight at 28℃ and 200 rpm with shaking. 1 mL of the bacterial suspension was inoculated into 50 mL of organic phosphorus medium (without agar), and 1 mL of sterile water was inoculated into the same medium as a control. The cultures were incubated at 28℃ and 180 rpm for a total of 5 days. Samples were taken at 24h, 48h, 72h, 96h, and 120h to measure pH and soluble phosphorus content using the molybdenum-antimony colorimetric method. Three replicates were performed for both the bacterial strain and the control.

[0067] The results are as follows Figure 3 and Figure 4 As shown, the pH of the culture medium dropped to around 4 one day after inoculation with BST187, and remained between 3 and 4 for five days. After five days of culture, the soluble phosphorus content in the culture medium reached as high as 7.81 mg / L, which was 4.02 mg / L after subtracting the control.

[0068] Example 4: Determination of the antibacterial function of strain BST187

[0069] This example uses the plate confrontation culture method to detect the antibacterial function of the highly efficient phosphate-solubilizing bacterium BST187. Plate antagonism experiments were conducted against three plant pathogenic bacteria listed in Table 1: *Xanthomonas campestris* pv. vesicatoria (causing pepper spot disease), *Ralstonia solanacearum*, and *Dickeyasolani*. The specific procedure was as follows: the pathogenic bacteria were activated and multiplied until the bacterial suspension concentration reached 10⁻⁶. 9 CFU / mL, mix evenly into unsolidified LB solid medium at a ratio of 1:100, and pour the medium to prepare a culture medium containing bacteria; take 2 μL of bacterial suspension of strain BST187 (concentration of 10). 9 Three drops of CFU / mL were added to each LB agar plate containing the bacteria. After incubation at 28°C for 24 hours, the diameter of the inhibition zone was measured. The biocontrol efficacy was compared between the commercially available biocontrol strain "QST713" and the biocontrol strain "BST23" preserved in our laboratory. Three replicates were performed for each pathogen.

[0070] For the antagonistic experiment against fungi, the pathogenic fungus was first activated on a PDA plate. A 5mm perforator was used to prepare the pathogenic fungus suspension, which was then inoculated into the center of the PDA plate. 2 μL of strain BST187 suspension was then transferred using a pipette (10... 9Incubate the bacterial culture (CFU / mL) at 2cm from the bacterial cell at 25℃ for 4-7 days. Measure the diameter of the pathogen in the control group and the treatment group respectively, and calculate the inhibition rate. The formula is: Inhibition rate = (Control colony diameter - Diameter of antagonistic bacteria in the treatment group) / Control colony diameter.

[0071] The results are as follows Figure 5 As shown, strain BST187, in addition to its highly efficient phosphorus-solubilizing function, also exhibits highly effective antibacterial effects against plant pathogenic bacteria. Furthermore... Figure 6 , 7 The results showed that, compared with strains QST713 and BST23, the antibacterial effect was significantly superior in inhibiting pathogenic bacteria Ds.

[0072] Example 5: Salt tolerance test of strain BST187

[0073] LB broth media with different salt (NaCl) concentrations were prepared: 1%, 3%, 5%, 7%, 10%, 13%, 15%, and 20%. The preserved bacterial strains were removed from a -80°C freezer, streaked onto LB solid medium for activation, and single colonies were picked and inoculated into LB liquid medium. The cultures were then incubated overnight at 28°C and 200 rpm with shaking. The bacterial suspensions were then added to LB broth media with different salt concentrations at a 1:100 ratio and incubated overnight at 28°C and 200 rpm with shaking. The growth of the bacterial strains was then observed.

[0074] The results showed that strain BST187 could grow normally in LB medium with a salt concentration of 10% or less without the medium becoming turbid.

[0075] Example 6: Preliminary description and activity verification of active substances

[0076] First, the BST187 bacterial strain was removed from the -80℃ freezer and streaked onto LB solid medium for activation, then incubated overnight at 28℃. The next day, single colonies were inoculated into 5 mL of LB liquid medium and incubated overnight at 28℃ and 200 rpm to prepare a seed culture. The following day, the bacterial suspension was centrifuged at 10,000 rpm for 5 min, the supernatant was removed, and the precipitated bacterial cells were resuspended in sterile 0.85% physiological saline. The absorbance (OD) of the bacterial suspension at 600 nm was adjusted. 600 The value was approximately 1. The seed culture was inoculated into 100 mL of LB liquid medium at a ratio of 1:100 and incubated at 28°C and 200 rpm for 24 h.

[0077] After 24 hours of cultivation, the fermentation metabolites were centrifuged directly at 10,000 rpm for 10 minutes. The supernatant was filtered through a 0.22 μm filter to remove bacterial cells. Liquid LB, treated in the same manner, served as a control. 80% ammonium sulfate was added to the cell-free metabolites, and the mixture was slowly stirred until dissolved. A brown solid precipitated after the addition of ammonium sulfate. The mixture was incubated overnight at 4°C. The next day, the mixture was centrifuged at 10,000 rpm for 10 minutes at 4°C. The supernatant was discarded, and the precipitate volume was weighed. Methanol was added to dissolve the precipitate to a concentration of 0.1 g / mL. After dissolution, the mixture was centrifuged at 10,000 rpm for 5 minutes at 4°C, and the supernatant was collected. The supernatant was brownish-yellow.

[0078] The inhibition zone method was used to determine the antibacterial effect of crude angiotensin extract, obtained after ammonium sulfate precipitation from BST187 fermentation metabolites, against pathogenic bacteria. The pathogenic bacteria were activated and propagated to a bacterial suspension concentration of 10... 9 CFU / mL was uniformly mixed into unsolidified LB broth at a ratio of 1:100, and the medium was poured to prepare a culture medium for bacterial contamination. After the medium solidified, three wells were punched in the medium using a 5 mm punch, and the bottoms were sealed with unsolidified LB broth to prevent loss of the test solution. 30 μL of the test solution was added to each well. After adding the test solution, the wells were placed face up in an incubator at 28°C overnight. LB extract was used as a negative control, and each treatment was repeated in triplicate.

[0079] The results are as follows Figure 9 The antibacterial results showed that the crude extract of angicin had a significant inhibitory effect on three pathogenic bacteria.

[0080] Example 7: Identification of Active Substances

[0081] The BST187 genome was submitted to Antismash for analysis of secondary metabolite gene clusters. The results are as follows: Figure 10 As shown, the whole genome of BST187 contains NRP: β-lactam+Polyketide (non-nucleotide: macrolide / polyketide) polypeptide andrimid, which has 100% similarity to the pantoea agglomerans Eh335.

[0082] To identify angimin in the crude extract of active substances, we performed qualitative analysis using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) with a mobile phase of 40 / 60 water / methanol and a UV detection wavelength of 297 nm. The results are as follows: Figure 11 and 12 As shown, mass spectrometry results, through molecular formula lookup and secondary fragment ion structure analysis, identified the substance with a peak elution time of 23.159-25.997 min as andrimid (C27H33N3O5). The content of andrimid in the crude fermentation extract was calculated to be 53.4% ​​using the area normalization method.

[0083] The number of modules and processing scale described herein are for the purpose of simplifying the description of the invention. Applications, modifications, and variations of the invention will be readily apparent to those skilled in the art.

[0084] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. Erwinia pulvinata strain 2022TIBBST187, its classification name: Erwinia pulvinata Erwinia persicina strain Erwinia peachis Erwinia persicina 2022TIBBST187 has been deposited at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M 2022872, deposit date: June 13, 2022, and address of the depository: Wuhan University, Wuhan, China.

2. A method for producing Erwinia peachensis, characterized in that, Includes the following steps: Erwinia pink as described in claim 1 Erwinia persicina The 2022TIBBST187 strain was inoculated into LB liquid medium and cultured to obtain fermentation broth.

3. The production method as described in claim 2, characterized in that, The culture was carried out at a temperature of 28°C and a rotation speed of 200 rpm for 24 hours.

4. The production method as described in claim 2, characterized in that, The process also includes the following steps: removing the bacterial cells from the fermentation broth obtained in production to obtain a supernatant, adding ammonium sulfate to the supernatant to a final concentration of 80% (w / v) and letting it sit overnight, then collecting the precipitate, adding methanol to dissolve the precipitate to a concentration of 0.1 g / mL, and obtaining a crude extract of angiin.

5. The application of Erwinia oxyphylla 2022TIBBST187 strain as described in claim 1 in biological control and stress resistance, characterized in that, The stress resistance is characterized by salt tolerance in the *Erwinia rosenbergii* 2022TIBBST187 strain, and the *Erwinia rosenbergii* 2022TIBBST187 strain is capable of solubilizing organic phosphorus compounds. The biological control refers to the application of Erwinia peachensis strain 2022TIBBST187 in inhibiting the activity of bacterial pathogens, wherein the bacterial pathogens are Ralstonia solanacearum (pepper spot disease pathogen), Ralstonia solanacearum, and Dichotomyces solani.

6. The application as described in claim 5, characterized in that, The applications include the production of andrimid and the use of andrimid in inhibiting the activity of bacterial pathogens, wherein the bacterial pathogens are *Ralstonia solanacearum* (pepper spot disease pathogen), *Ralstonia solanacearum*, and *Dicotyle solanacearum*.

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