Zebrafish full-automatic precision feeding breeding system and use method

Abstract

The scheme discloses a zebrafish full-automatic precision feeding breeding system and a use method thereof, and comprises a worm breeding unit for hatching brine shrimp eggs; and a feeding unit for feeding the brine shrimp eggs bred by the worm breeding unit into a breeding container of zebrafish. The brine shrimp eggs can be automatically bred, and the zebrafish can be bred through the brine shrimp eggs, so that the breeding effect is obviously improved and the cost is reduced. The system has the ability of stable self-purification and sewage reduction, reduces the artificial breeding maintenance cost, and improves the stability and durability of the breeding environment.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of automatic breeding equipment, in particular to a zebrafish full-automatic precision feeding breeding system and a use method thereof. BACKGROUND

[0002] Zebrafish (Danio rerio) is a small tropical freshwater fish of the family Cyprinidae and genus Danio, which is famous for being widely used in scientific experiments. Its advantages are fast growth, short breeding cycle and other excellent physiological characteristics, which can meet the needs of a large number of scientific research in a short time. Female zebrafish can reproduce every two weeks, and each time can produce hundreds of eggs. At the same time, the egg granules have a short incubation period and transparent egg membranes, which are easy to observe. As a model organism, zebrafish genes have high homology with human genes, and the genome size is about 150 million base pairs, about 70% of which are highly conserved with human genes, which makes the research results based on zebrafish as a research model highly recognized. In addition, the genetic information and genome sequence of zebrafish have been completely analyzed, so it has a good application basis in the fields of medicine, toxicology, etc.

[0003] In order to ensure that zebrafish has good growth and development before being used for research and application, stable feed source and feeding environment are very important. Among them, as a kind of high nutritional value feed, brine shrimp eggs have been widely used in the standardized breeding of zebrafish. However, the traditional full-manual brine shrimp egg cultivation and feeding method of zebrafish has certain limitations, such as complicated operation, low feed utilization efficiency, low feeding amount precision, etc. SUMMARY

[0004] In order to solve the above technical problems, the present application provides a zebrafish full-automatic precision feeding breeding system and a use method thereof, which can realize automatic cultivation of brine shrimp eggs, and further cultivate zebrafish through brine shrimp eggs, thereby significantly improving the breeding effect and reducing the cost, having stable self-purification and sewage discharge capacity, reducing the cost of artificial breeding and maintenance, and improving the stability and durability of the breeding environment.

[0005] The technical scheme adopted by the present application to solve the technical problems is: a worm culture unit for hatching brine shrimp eggs;

[0006] A feeding unit for feeding the brine shrimp cultured by the worm culture unit into the zebrafish breeding container.

[0007] Through the present application, the effect of cultivating zebrafish by culturing brine shrimp eggs can be realized.

[0008] Preferably, the worm culture unit comprises a worm culture barrel and an aeration device.

[0009] The bottom of the worm culture barrel is provided with a bait discharge port, the bait discharge port is provided with an electric control valve, the bait discharge port is communicated to the bait feeding unit through a bait discharge pipeline, the sidewall of the worm culture barrel above the bait discharge port is provided with an aeration port, and the aeration port is communicated to the aeration device through an aeration pipe;

[0010] The middle part of the worm culture barrel is provided with a partition plate, the lower end of the partition plate is connected to the inside of the worm culture barrel, the sidewall of the worm culture barrel above the lower end of the partition plate is provided with a water inlet, the water inlet is communicated to a water inlet pipe, a screening gap is arranged between the upper end of the partition plate and the inner wall of the worm culture barrel, and the sidewall of the worm culture barrel above the screening gap is provided with a sewage discharge port, and the sewage discharge port is communicated to a sewage discharge pipe.

[0011] The sidewall of the worm culture barrel is provided with a heating device and a lighting device.

[0012] Preferably, the sidewall of the worm culture barrel is provided with an egg injection port and a salt adding port.

[0013] Preferably, the bait feeding unit comprises a culture container, a lead screw, a sliding rail, a moving block and a metering cavity.

[0014] A plurality of culture containers are arranged in a straight line, the lead screw and the sliding rail are arranged in parallel along the distribution straight line of the culture containers, the lead screw is driven to rotate by a motor, the moving block is threadedly matched with the lead screw, the moving block is slidably matched with the sliding rail, the moving block is provided with the metering cavity, the metering cavity is communicated to the bait discharge pipeline, and the metering cavity is provided with a worm injection pipe for feeding the culture container.

[0015] Preferably, the culture container is provided with a sensing part, and the moving block is provided with a position sensor for detecting the sensing part.

[0016] Preferably, the front end and the rear end of the moving block are respectively provided with proximity switches, and when the proximity switches detect an object at the end, the motor drives the lead screw in the reverse direction to drive the moving block to move.

[0017] Preferably, the moving block is provided with a rudder, the output end of the rudder is fixed with the worm injection pipe, the worm injection pipe is communicated to the metering cavity through a hose, and the rudder drives the worm injection pipe to swing upward or downward to dock to the culture container.

[0018] Preferably, the metering cavity is provided with a liquid level sensor, and when the liquid level sensor detects the water level, the electric control valve is closed.

[0019] A use method of a zebrafish full-automatic precise bait feeding and breeding system, breeding brine shrimp eggs, providing heating, lighting, water inlet, water discharge, air supply and salt adding operations according to the physiological needs of brine shrimp egg hatching.

[0020] Screening of Daphnia egg: through the aeration device, the gas supply in the culture barrel makes the shell float above the partition, the Daphnia egg sinks to form a Daphnia egg suspension, the water inlet pipe enters water to impact, the Daphnia in the partition is washed to the lower part, and the shell on the water surface is washed out to the sewage outlet;

[0021] Daphnia egg feeding: the rudder drives the egg injection pipe to swing upward, opens the electric control valve, injects the Daphnia egg suspension into the metering cavity, when the water level sensor detects the water level, closes the electric control valve, and the rudder drives the egg injection pipe to swing downward and is connected to the culture container, and the Daphnia egg suspension is discharged into the culture container.

[0022] The beneficial effects of the present application: the present application can realize the automatic culture of Daphnia egg, and further realize the effect of Daphnia egg breeding zebrafish, significantly improve the breeding effect and reduce the cost, have the ability of stable self-purification and sewage discharge, reduce the maintenance cost of artificial breeding, and improve the stability and durability of the breeding environment. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 A front view of a zebrafish automatic precise feeding breeding system is provided in the present application;

[0024] Figure 2 A rear view of a zebrafish automatic precise feeding breeding system is provided in the present application;

[0025] Figure 3 A cross-sectional view of a Daphnia culture system contained in a zebrafish automatic precise feeding breeding system is provided in the present application;

[0026] Figure 4 A front view of a Daphnia culture system contained in a zebrafish automatic precise feeding breeding system is provided in the present application;

[0027] In the figure: 1 waste liquid cylinder, 2 culture system, 3 controllable feeding module, 4 screw rod, 5 magnet piece, 6 zebrafish culture cylinder, 7 master control station, 8 lamp strip, 9 culture partition, 10 water inlet, 11 heating disc, 12 bait discharge pipe, 13 water outlet, 14 aeration pump, 15 egg injection port, 16 salt inlet, 17 air inlet pipe. DETAILED DESCRIPTION

[0028] In order to deepen the understanding of the present application, the present application will be further described in detail below in combination with the drawings and examples, which are only used to explain the present application and do not limit the protection scope of the present application. EMBODIMENT

[0029] A zebrafish full-automatic precise feeding breeding system and a using method, comprising the following:

[0030] Culture unit 2: for hatching Daphnia egg;

[0031] Feeding unit 3: for feeding the brine shrimp cultivated by the brine shrimp cultivation unit 2 into the breeding container 6 of the zebrafish.

[0032] The present application can achieve the effect of cultivating zebrafish by cultivating brine shrimp eggs.

[0033] The brine shrimp cultivation unit 2 comprises a brine shrimp barrel and an aeration device 14; the brine shrimp barrel is made of acrylic material, the lower end of the brine shrimp barrel is in a conical structure, the lower end of the conical structure is provided with a bait discharge port 12, the bait discharge port 12 is provided with an electric control valve, the bait discharge port 12 is communicated to the feeding unit 3 through a bait discharge pipeline, the side wall of the brine shrimp barrel above the bait discharge port 12 is provided with an aeration port, and the aeration port is communicated to the aeration device 14 through an aeration pipe.

[0034] The middle part of the brine shrimp barrel is provided with a partition plate 9 at an angle of 40 degrees with the horizontal plane, the lower end of the partition plate 9 is connected to the inside of the brine shrimp barrel, the side wall of the brine shrimp barrel corresponding to the upper end of the partition plate 9 is provided with a water inlet 10, the water inlet 10 is communicated with a water inlet pipe, a screening gap is left between the upper end of the partition plate 9 and the inner wall of the brine shrimp barrel, the side wall of the brine shrimp barrel corresponding to the upper end of the screening gap is provided with a sewage discharge port 13, and the sewage discharge port 13 is communicated with a sewage discharge pipe. The sewage discharge pipe is communicated to the waste liquid barrel 1.

[0035] The side of the brine shrimp barrel is provided with a heating device 11 and a lighting device 8. The heating device 11 adopts a waterproof short-circuit heating device, and the existing commonly used water-electric separation heating device is adopted. The lighting device 8 is a lighting strip arranged at the top of the brine shrimp barrel.

[0036] The side wall of the brine shrimp barrel is provided with an egg injection port 15 and a salt adding port 16. The egg injection port 15 is located above the screening gap. The aeration port is located below the screening gap. The brine shrimp eggs can be added to the brine shrimp barrel through the egg injection port 15, and the concentration of salt in the interior can be adjusted through the salt adding port 16.

[0037] The feeding unit 3 comprises a breeding container 6, a lead screw 4, a sliding rail, a moving block and a metering cavity.

[0038] A plurality of breeding containers 6 are arranged in a straight line, the lead screw 4 and the sliding rail are arranged in parallel according to the distribution straight line of the breeding container 6, the lead screw 4 is driven to rotate by a motor, the moving block is threadedly connected with the lead screw 4, the moving block is slidably connected with the sliding rail, the moving block is provided with the metering cavity, the metering cavity is communicated with the bait discharge pipeline, and the metering cavity is provided with a brine shrimp injection pipe for feeding the breeding container 6. Through the cooperation of the lead screw 4 and the sliding rail, the moving block can be moved, and then the metering cavity can be stopped at the corresponding breeding container 6 to add brine shrimp egg suspension to the breeding container 6 to cultivate zebrafish.

[0039] The breeding container 6 is respectively provided with a sensing part, and the moving block is provided with a positioning sensor. The sensing part is a magnet piece 5, and the positioning sensor is a Hall sensor. After the Hall sensor detects the magnet piece 5, the control motor stops rotating, so that the moving block stays at the corresponding breeding container 6,

[0040] The front end and the rear end of the moving block are respectively provided with a proximity switch. When the proximity switch detects an object at the end, the motor drive screw 4 is controlled to drive the moving block in the opposite direction. The moving block is controlled to move within the range formed by the screw 4, so as to avoid the screw 4 from driving the moving block to collide with the structure at both ends.

[0041] The moving block is provided with a rudder, and the output end of the rudder is fixed with the worm injection pipe. The worm injection pipe is communicated with the metering cavity through a hose. The rudder drives the worm injection pipe to swing upward or downward to dock to the breeding container 6. The metering cavity is provided with a liquid level sensor. When the liquid level sensor detects the water level, the electric control valve is closed. After the brine shrimp egg suspension is injected into the metering cavity, the liquid level sensor detects the liquid level, controls the electric control valve to be closed, stops adding the brine shrimp egg suspension into the metering cavity, and then the rudder drives the worm injection pipe to swing downward. The brine shrimp egg suspension in the metering cavity flows into the breeding container 6. In this way, the brine shrimp egg suspension in the breeding container 6 is measured by the metering cavity, and the effect of quantitative feeding of the brine shrimp egg suspension is achieved.

[0042] In order to improve the overall automation performance, a total control unit is arranged. The total control unit includes a PLC with a preset program, receives models of all sensors, processes, and controls controllable devices.

[0043] A use method of a zebrafish full-automatic precise feeding breeding system. The brine shrimp eggs are bred according to the physiological requirements of the brine shrimp eggs. Heating, lighting, water inlet, water outlet, gas supply and salt adding operations are provided.

[0044] Screening of brine shrimp eggs: the gas supply device 14 supplies gas to the culture barrel to make the shells float above the baffle 9, and the brine shrimp eggs sink to form a brine shrimp egg suspension. The water inlet pipe supplies water to impact and flush the organisms on the baffle 9 to the lower side. The shells on the water surface are flushed to the sewage outlet 13.

[0045] Brine shrimp egg feeding: before the brine shrimp egg feeding, gas needs to be injected into the culture barrel. The gas mixes the brine shrimp eggs and water uniformly. Then the rudder drives the worm injection pipe to swing upward, the electric control valve is opened, the brine shrimp egg suspension is injected into the metering cavity, the liquid level sensor detects the water level, the electric control valve is closed, the rudder drives the worm injection pipe to swing downward to dock to the breeding container 6, and the brine shrimp egg suspension is discharged into the breeding container 6.

[0046] The Daphnia magna egg enters into the culture barrel through the egg injection port 15, and sinks to the lower side of the baffle 9 under the action of gravity, and under the inflation of the aeration port, the Daphnia magna egg is driven to surge and hatch, in the hatching process, the baffle 9 isolates the Daphnia magna egg, so that the Daphnia magna egg cannot enter the upper side of the baffle 9, after the hatching is completed, the surging is stopped, the worm body sinks, the worm shell floats along the baffle, and the worm shell is separated through the screening gap, a small amount of worm body enters the upper side of the baffle 9, and after water flushing at the water inlet 10, the worm body sinks to the lower side of the baffle 9 through the screening gap, so that the loss of the Daphnia magna egg is reduced, and the density of the Daphnia magna egg suspension is accurately ensured.

[0047] Culture Daphnia abundance:

[0048] The Daphnia magna egg density is 4-4.5g / L, the suspension volume in the culture system is recorded as an effective volume, and the calculation formula is V_0=1 / 2 Nk, wherein V_0 is the effective volume, indicating the volume of the worm body suspension in the barrel, N is the zebrafish breeding amount, and k is the feeding constant, that is, the daily feeding frequency, and is usually 2-3. According to this, the culture barrel volume is designed, and the culture suspension volume expansion caused by aeration and the baffle volume in the culture system are considered. The calculation formula is V=γV_0, wherein γ is a constant, and the constant is 1.2-1.5.

[0049] The dormant Daphnia magna egg hatches into adult, and the hatching effect has significant correlation with environmental parameters, the system adopts light intensity 2000Lx, salinity 10‰, water temperature 28℃, and slow aeration culture for 26h.

[0050] The beneficial effects of the present application are as follows: the present application can realize automatic culture of Daphnia magna eggs, and further realize the effect of breeding zebrafish through Daphnia magna eggs, significantly improve the breeding effect and reduce the cost, have the ability of stable self-purification and sewage discharge, reduce the artificial breeding maintenance cost, and improve the stability and durability of the breeding environment.

[0051] The present scheme can reduce human errors, control feeding time, quantity and frequency, reduce inconsistencies caused by manual operation, and improve the reliability and standardization of experimental animals. The automatic feeding device can reduce the interaction between humans and experimental animals, effectively reduce the stress response of experimental animals, and help maintain the stability of experimental results. In terms of hatching of abundant shrimp eggs and feeding of zebrafish, it has significant advantages, providing a more efficient, accurate and reliable solution for biological research, thereby improving the accuracy of experimental quality and research results. In addition, it helps to reduce experimental costs, especially in large-scale feeding modes, the advantages of automated management will be more obvious. It can be customized for laboratory breeding of experimental animals (such as zebrafish), making the device more suitable for the actual needs of the laboratory, ensuring precise control and efficient operation of feeding during the experiment; it can achieve controllable, accurate and precise feeding at specific points, improving breeding efficiency and reducing costs; it has stable self-cleaning and sewage discharge capacity, reducing manual maintenance costs and improving the stability and durability of the breeding environment; it realizes the versatility and scalability of feed, and can flexibly adjust the feeding mode according to different breeding needs.

[0052] The above examples should not limit the present application in any way, and any technical solutions obtained by equivalent substitution or equivalent conversion fall within the scope of the present application.

Claims

1. A full-automatic and precise feeding system for breeding zebrafish, characterized in that: a breeding unit (2) is used for hatching brine shrimp eggs; a feeding unit (3) is used for feeding the brine shrimp bred in the breeding unit (2) into a breeding container (6) for zebrafish; the breeding unit (2) comprises a breeding barrel and an aeration device (14); a bottom of the breeding barrel is provided with a brine shrimp outlet (12) provided with an electric control valve, the brine shrimp outlet (12) is communicated to the feeding unit (3) through a brine shrimp pipeline, and a sidewall of the breeding barrel above the brine shrimp outlet (12) is provided with an aeration opening communicated to the aeration device (14) through an aeration pipeline; a middle part of the breeding barrel is provided with a partition plate (9) inclinedly arranged, a lower end of the partition plate (9) is connected to an inner part of the breeding barrel, a water inlet (10) is arranged on a sidewall of the breeding barrel above the lower end of the partition plate (9), the water inlet (10) is communicated to a water inlet pipeline, a screening gap is arranged between an upper end of the partition plate (9) and an inner wall of the breeding barrel, and a sewage outlet (13) is arranged on a sidewall of the breeding barrel above the screening gap, and the sewage outlet (13) is communicated to a sewage pipeline. A heating device (11) and a lighting device (8) are arranged on a side of the breeding barrel. An egg injection opening (15) and a salt adding opening (16) are arranged on a sidewall of the breeding barrel. The feeding unit (3) comprises the breeding container (6), a lead screw (4), a sliding rail, a moving block and a metering cavity. A plurality of breeding containers (6) are arranged in a straight line in sequence, the lead screw (4) and the sliding rail are arranged in parallel along the distribution straight line of the breeding containers (6), the lead screw (4) is driven to rotate by a motor, the moving block is threadedly matched with the lead screw (4), the moving block is slidingly matched with the sliding rail, the moving block is provided with the metering cavity, the metering cavity is communicated to the brine shrimp pipeline, and the metering cavity is provided with a brine shrimp injection pipeline for feeding the breeding container (6). The moving block is provided with a positioning sensor for detecting the inductive element.

2. The zebrafish automatic precision feeding and breeding system according to claim 1, characterized in that, Proximity switches are arranged at front and rear ends of the moving block, and when the proximity switches detect objects at the ends, the motor drives the lead screw (4) in the reverse direction to drive the moving block to move.

3. The zebrafish automatic and precise feeding and breeding system according to claim 2, characterized in that, The moving block is provided with a steering engine, an output end of the steering engine is fixed with the brine shrimp injection pipeline, the brine shrimp injection pipeline is communicated to the metering cavity through a hose, and the steering engine drives the brine shrimp injection pipeline to swing upward or downward to be docked to the breeding container (6).

4. The full-automatic and precise zebrafish feeding and breeding system according to claim 3, characterized in that, The metering cavity is provided with a liquid level sensor, and the liquid level sensor is closed when detecting the water level. ​ 5. The zebrafish automatic precision feeding and breeding system according to claim 4, characterized in that, ​ 6. The zebrafish automatic precision feeding and breeding system according to claim 5, characterized in that, ​ 7. The zebrafish automatic precision feeding and breeding system according to claim 6, characterized in that, ​ 8. The zebrafish automatic precision feeding and breeding system according to claim 7, characterized in that, ​

Images