Lysis tube, biological sample lysis device and nucleic acid detection device

Abstract

The application provides a cracking tube, a biological sample cracking device and a nucleic acid detection device. The cracking tube comprises a tube body and a spoiler structure. The spoiler structure is arranged on the inner wall of the tube body and protrudes relative to the inner wall. According to the application, the spoiler structure is arranged on the inner wall of the tube body, so that the cracking efficiency is improved and the cracking is more sufficient.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid detection instrument technology, and in particular to a lysis tube, a biological sample lysis device, and a nucleic acid detection device. Background Technology

[0002] Lysis devices, as an essential component of nucleic acid detection instruments, have always been a popular research area in nucleic acid testing. However, most existing lysis devices are developed to match large-scale detection equipment, which often results in nucleic acid loss, low lysis degree, or low automation while increasing the number of tests.

[0003] Common technical challenges in existing pyrolysis technologies include improving pyrolysis efficiency, increasing automation, ensuring equipment convenience, enhancing safety, and improving pyrolysis precision and purity. Existing pyrolysis equipment primarily achieves ideal grinding conditions by controlling the vibration frequency of the grinding device, the ratio of the pyrolysis solution, and the fit between the grinding cup and grinding beads; this is a commonly used technique in physical pyrolysis. However, meeting the requirements for high-precision and rapid detection in small-scale equipment is often difficult. Summary of the Invention

[0004] A primary objective of this invention is to overcome at least one of the deficiencies of the prior art and to provide a pyrolysis tube that provides more complete pyrolysis and higher efficiency.

[0005] Another major objective of the present invention is to overcome at least one of the defects of the prior art described above and to provide a biological sample lysis device having the above-described lysis tube.

[0006] Another major objective of the present invention is to overcome at least one of the defects of the prior art and to provide a nucleic acid detection device having the above-mentioned biological sample lysis device.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] According to one aspect of the present invention, a pyrolysis tube is provided, comprising a tube body and a flow-disrupting structure; the flow-disrupting structure is disposed on the inner wall of the tube body and protrudes relative to the inner wall.

[0009] According to another aspect of the present invention, a biological sample lysis device is provided, comprising a support and at least one vortex mixing unit; the vortex mixing unit is disposed on the support, each of the vortex mixing units comprising a clamping seat and a lysis tube as proposed in the present invention and described in the above embodiments; the clamping seat is rotatably disposed on the support by a first driving member; the lysis tube is disposed on the clamping seat and is used to hold abrasive particles and sample solution.

[0010] According to another aspect of the present invention, a nucleic acid detection device is provided, comprising the biological sample lysis device proposed in the present invention and described in the above embodiments.

[0011] As can be seen from the above technical solutions, the advantages and positive effects of the lysis tube, biological sample lysis device, and nucleic acid detection device proposed in this invention are as follows:

[0012] The pyrolysis tube proposed in this invention improves pyrolysis efficiency and makes pyrolysis more complete by using a turbulence-inducing structure protruding from the inner wall of the tube.

[0013] The biological sample lysis device proposed in this invention includes a support, a vortex mixing unit, and a reset assembly. The vortex mixing unit is disposed on the support, and each vortex mixing unit includes a clamping seat and a lysis tube. The clamping seat is rotatably mounted on the support. The lysis tube is disposed on the clamping seat. The reset assembly is disposed on the support and is used to reset the clamping seat after rotation, returning it to its initial position before rotation. Through the above design, the biological sample lysis device proposed in this invention can achieve rapid and active reset of the lysis tube through the reset assembly, which helps to reduce operation steps, improve the automation level of the equipment, and achieve rapid and accurate detection functions. Attached Figure Description

[0014] Various objects, features, and advantages of the invention will become more apparent from the following detailed description of preferred embodiments of the invention, taken in conjunction with the accompanying drawings. The drawings are merely illustrative of the invention and are not necessarily drawn to scale. In the drawings, the same reference numerals always denote the same or similar parts. Wherein:

[0015] Figure 1 This is a three-dimensional structural schematic diagram of a biological sample lysis apparatus according to an exemplary embodiment;

[0016] Figure 2 yes Figure 1 An enlarged schematic diagram of a portion of the biological sample lysis device is shown.

[0017] Figure 3 yes Figure 1 Axial cross-sectional view of the lysis tube of the biological sample lysis device shown;

[0018] Figure 4 and Figure 5 These are axial sectional views of the lysis tubes of a biological sample lysis apparatus shown according to several other exemplary embodiments.

[0019] The annotations in the attached figures are explained as follows:

[0020] 100. Bracket;

[0021] 110. Top plate;

[0022] 200. Vortex mixing unit;

[0023] 210. First driving component;

[0024] 211. Eccentric shaft;

[0025] 220. Clamping seat;

[0026] 221. First magnetic component;

[0027] 222. Gripper;

[0028] 230. Pyrolysis tube;

[0029] 231. Pipe body;

[0030] 232. Capping;

[0031] 233. Turbulence structure;

[0032] 240. Vibration damping components;

[0033] 300. Reset assembly;

[0034] 310. Second magnetic component;

[0035] 320. Second driving component;

[0036] 330. Fixture;

[0037] 340. Guide rod;

[0038] D0. Inner diameter;

[0039] D1. Distance;

[0040] D2. Distance;

[0041] H0. Height;

[0042] H1. Height;

[0043] L1. Length. Detailed Implementation

[0044] Typical embodiments embodying the features and advantages of the present invention will be described in detail in the following description. It should be understood that the present invention can have various variations in different embodiments without departing from the scope of the present invention, and the description and drawings therein are for illustrative purposes only and not intended to limit the present invention.

[0045] In the following description of different exemplary embodiments of the invention, reference is made to the accompanying drawings, which form part of the invention, and in which different exemplary structures, systems, and steps that can implement various aspects of the invention are shown by way of example. It should be understood that other specific embodiments of the components, structures, exemplary devices, systems, and steps may be used, and structural and functional modifications may be made without departing from the scope of the invention. Furthermore, although the terms “above,” “between,” “within,” etc., may be used in this specification to describe different exemplary features and elements of the invention, these terms are used herein only for convenience, such as the orientation according to the examples shown in the drawings. Nothing in this specification should be construed as requiring a specific three-dimensional orientation of the structure to fall within the scope of the invention.

[0046] See Figure 3 The diagram illustrates a representative axial sectional view of the pyrolysis tube 230 proposed in this invention. In one embodiment, the pyrolysis tube 230 may include a tube body 231 and a flow-dispersing structure 233. Specifically, the tube body 231 has an opening at its top. The flow-dispersing structure 233 is disposed on the inner wall of the tube body 231, and protrudes relative to the inner wall of the tube body 231. Through the above design, this invention can improve pyrolysis efficiency and achieve more complete pyrolysis by utilizing the pyrolysis tube 230 with the flow-dispersing structure 233. For example, through actual comparison, compared with existing pyrolysis devices, under the same motor (first drive member 210) speed, the pyrolysis tube 230 of this invention using the flow-dispersing structure 233 can save 5% to 15% of the pyrolysis time. Alternatively, in the same pyrolysis time, for example, 5 minutes, the pyrolysis efficiency of this invention can be increased by 5% to 15%.

[0047] In one embodiment of the present invention, the pyrolysis tube 230 may include a plurality of turbulence structures 233, and the plurality of turbulence structures 233 may be arranged at circumferential intervals along the tube body 231.

[0048] In one embodiment of the present invention, when the pyrolysis tube 230 includes a plurality of turbulence structures 233, the plurality of turbulence structures 233 can be uniformly arranged at circumferential intervals along the tube body 231.

[0049] In one embodiment of the present invention, the number of turbulence-inducing structures 233 in a single pyrolysis tube 230 can be 2 to 15, for example, 2, 8, 12, or 15. In some embodiments, the number of turbulence-inducing structures 233 in a single pyrolysis tube 230 can also be more than 15, for example, 16 or 20. Furthermore, the pyrolysis tube 230 may also have only one turbulence-inducing structure 233, and these are not limiting.

[0050] like Figure 3As shown, in one embodiment of the present invention, the width of the top end of the turbulence structure 233 along the radial direction of the tube body 231 can be greater than the width of its bottom end.

[0051] like Figure 3 As shown, in one embodiment of the present invention, the shape of the axial side profile of the turbulence-disrupting structure 233 along the axial direction of the tube body 231 can be generally stepped, such as the stepped shape including three "steps" shown in the figure. In some embodiments, the shape of the axial side profile of the turbulence-disrupting structure 233 along the axial direction of the tube body 231 can also be rectangular, triangular, trapezoidal, hemispherical, semi-ellipsoidal, wavy, sawtooth, or other shapes, and is not limited thereto.

[0052] See Figure 4 , Figure 4 The image shows a representative axial sectional view of the lysis tube 230 of a biological sample lysis apparatus in another embodiment.

[0053] like Figure 4 As shown, in one embodiment of the present invention, each turbulence structure 233 includes a plurality of hemispherical structures arranged along the axial direction of the tube body 231.

[0054] See Figure 5 , Figure 5 The image shows a representative axial sectional view of the lysis tube 230 of a biological sample lysis apparatus in another embodiment.

[0055] like Figure 5 As shown, in one embodiment of the present invention, each turbulence structure 233 is an inverted trapezoidal structure.

[0056] like Figure 3 As shown, in one embodiment of the present invention, along the axial direction of the pipe body 231, the proportion of the height H1 of the turbulence-disrupting structure 233 in the height H0 of the pipe body 231 can be 30% to 95%, for example, 30%, 45%, 70%, 95%, etc. In some embodiments, the proportion of the height H1 of the turbulence-disrupting structure 233 in the height H0 of the pipe body 231 can also be less than 30% or greater than 95%, for example, 25%, 96%, etc., and is not limited thereto.

[0057] like Figure 3 As shown, in one embodiment of the present invention, the length L1 of the turbulence-disrupting structure 233 in the inner diameter D0 of the pipe body 231 along the radial direction is 15% to 40%, for example, 15%, 20%, 30%, 40%, etc. In some embodiments, the length L1 of the turbulence-disrupting structure 233 in the inner diameter D0 of the pipe body 231 may be less than 15% or greater than 40%, for example, 14%, 45%, etc., and is not limited thereto.

[0058] In one embodiment of the present invention, the width of the turbulence-disrupting structure 233 along the circumferential direction of the pipe body 231 can account for 5% to 40% of the inner circumference of the pipe body 231, for example, 5%, 10%, 25%, 40%, etc. In some embodiments, the width of the turbulence-disrupting structure 233 can also account for less than 5% or more than 40% of the inner circumference of the pipe body 231, for example, 4%, 45%, etc., and is not limited thereto.

[0059] like Figure 3 In one embodiment of the present invention, the distance D1 between the bottom end of the turbulence-disrupting structure 233 and the bottom end of the pipe body 231 along the axial direction of the pipe body 231 can account for 3% to 40% of the height H0 of the pipe body 231, for example, 3%, 15%, 30%, 40%, etc. In some embodiments, the distance D1 between the bottom end of the turbulence-disrupting structure 233 and the bottom end of the pipe body 231 can also account for less than 3% or more than 40% of the height H0 of the pipe body 231, for example, 2%, 45%, etc., and is not limited thereto.

[0060] like Figure 3 In one embodiment of the present invention, the distance D2 between the top end of the turbulence-disrupting structure 233 and the top end of the pipe body 231 along the axial direction of the pipe body 231 can account for 10% to 50% of the height H0 of the pipe body 231, for example, 10%, 20%, 35%, 50%, etc. In some embodiments, the distance D2 between the top end of the turbulence-disrupting structure 233 and the top end of the pipe body 231 can also account for less than 10% or more than 50% of the height H0 of the pipe body 231, for example, 8%, 55%, etc., and is not limited thereto.

[0061] In one embodiment of the present invention, the surface of the turbulence structure 233 may have protrusions, thereby further improving the pyrolysis efficiency and further ensuring sufficient pyrolysis.

[0062] In one embodiment of the present invention, the bottom area of ​​the surface protrusion of the turbulence structure 233 accounts for 20%-90% of the outer surface area of ​​the turbulence structure 233. In some embodiments, the bottom area of ​​the surface protrusion of the turbulence structure 233 may be less than 20% or greater than 90%, for example, 10%, 15%, 95%, etc., and is not limited thereto.

[0063] In one embodiment of the present invention, the height of the surface protrusion of the turbulence structure 233 ranges from 0.05 to 1 mm. In some embodiments, the height of the surface protrusion of the turbulence structure 233 may be less than 0.05 mm or greater than 1 mm, for example, 0.03 mm, 1.2 mm, etc., and is not limited thereto.

[0064] In one embodiment of the present invention, the turbulence structure 233 can be connected to the inner wall of the tube body 231 in a seamless contact manner, such as, but not limited to, welding, integral molding, hot-melt connection, etc.

[0065] In one embodiment of the present invention, the tube body 231 has an opening at the top, and the pyrolysis tube 230 further includes a cap 232 for sealing the opening. The cap 232 is provided with an inner membrane that seals the lumen of the tube body 231. Both the cap 232 and the inner membrane have cracks to allow a pipette tip to pass through.

[0066] Based on the design of an inner membrane provided inside the tube 231, in one embodiment of the present invention, the inner membrane can be made of a flexible material.

[0067] Based on the design of having an inner membrane inside the tube 231, in one embodiment of the present invention, the shape of the crack is straight, cross-shaped, arc-shaped, wavy, or any combination of the above.

[0068] like Figure 1 As shown, in one embodiment of the present invention, the biological sample lysis device proposed in this invention includes a support 100, at least one vortex mixing unit 200, and a reset assembly 300. (See also...) Figure 2 , Figure 2 The diagram above shows a partially enlarged schematic of the biological sample lysis device. The structure, connection method, and functional relationship of the main components of the biological sample lysis device proposed in this invention will be described in detail below with reference to the above-mentioned figures.

[0069] like Figure 1 As shown, in one embodiment of the present invention, the biological sample lysis device may include multiple vortex mixing units 200, which may be arranged sequentially along a certain direction. Each vortex mixing unit 200 is disposed on a support 100 and includes a clamping seat 220 and a lysis tube 230. The clamping seat 220 is rotatably disposed on the support 100 and can be driven by a first driving member 210. The lysis tube 230 is disposed on the clamping seat 220 and is used to hold the abrasive particles and sample solution. A reset component 300 is disposed on the support 100 and can reset the clamping seat 220 to its initial position. Through the above design, the biological sample lysis device proposed in this invention can achieve rapid and active reset of the lysis tube 230 through the reset component 300, which helps to reduce operation steps, improve the automation level of the equipment, and achieve rapid and accurate detection functions.

[0070] It should be noted that in some embodiments, the biological sample lysis device may include only one vortex mixing unit 200, and is not limited thereto.

[0071] like Figure 1 As shown, in one embodiment of the present invention, the clamping seat 220 is provided with a first magnetic element 221. Based on this, the reset assembly 300 may include a second magnetic element 310 and a second driving element 320. Specifically, the second magnetic element 310 is movably disposed on the bracket 100, and the second magnetic element 310 is located on the side of the clamping seat 220 where the first magnetic element 221 is disposed. The second driving element 320 is disposed on the bracket 100 and is throttle-connected to the second magnetic element 310. Accordingly, when the clamping seat 220 finishes working, the reset assembly 300 can drive the second magnetic element 310 closer to the clamping seat 220 via the second driving element 320, and use the magnetic attraction between the first magnetic element 221 and the second magnetic element 310 to reset the clamping seat 220. In some embodiments, other forms of reset structures may be used instead of the magnetic attraction reset design of the first magnetic element 221 and the second magnetic element 310, such as connecting rods, chains, etc., and are not limited thereto.

[0072] like Figure 1 As shown, in one embodiment of the present invention, the reset assembly 300 further includes a fixing base 330. Specifically, the fixing base 330 is fixed to the bracket 100, and the fixing base 330 is located on the side of the clamping base 220 where the first magnetic element 221 is provided. Furthermore, a guide rod 340 is connected to the side of the second magnetic element 310 facing away from the clamping base 220. The guide rod 340 is movably inserted through the fixing base 330 to provide guidance during the translation of the second magnetic element 310 relative to the fixing base 330. Additionally, a second driving element 320 may be disposed on the fixing base 330.

[0073] In one embodiment of the present invention, the second driving member 320 may be a permanent magnet stepper motor.

[0074] like Figure 1 As shown, in one embodiment of the present invention, the output end of the first driving member 210 may include an eccentric shaft 211. Furthermore, the eccentric shaft 211 can be connected to the clamping seat 220 via a flexible connector.

[0075] Based on the design of the eccentric shaft 211 of the first driving member 210 being connected to the clamping seat 220 via a flexible connector, in one embodiment of the present invention, the flexible connector may include a flexible connecting rope.

[0076] In one embodiment of the present invention, the connecting rope passes through the internal hole of the buckle and forms an annular area above the hole. The annular area is positioned directly above the eccentric shaft 211 of the clamping piece of the pyrolysis tube 230. The buckle is pushed forward to make the connecting rope fit tightly with the clamping piece of the pyrolysis tube 230. The two ends of the connecting rope are fixed to the side baffle of the clamping piece bracket 100 of the pyrolysis tube 230.

[0077] In one embodiment of the present invention, the first driving member 210 may be a DC motor.

[0078] like Figure 1 As shown, in one embodiment of the present invention, the clamping seat 220 may include a plurality of jaws 222, which are arranged at intervals around the clamping seat 220 in the circumferential direction, and are used to clamp the pyrolysis tube 230.

[0079] like Figure 1 As shown, in one embodiment of the present invention, the bracket 100 includes a top plate 110. Based on this, a clamping seat 220 is rotatably disposed on the top surface of the top plate 110, and a first driving member 210 is disposed below the bottom plate, with the output end of the first driving member 210 passing through the top plate 110 and connected to the clamping seat 220.

[0080] like Figure 2 In one embodiment of the present invention, the support 100 can be disposed on a base, for example, on the base of the housing of a nucleic acid detection device, with the top plate 110 located above the base, and the first driving member 210 disposed between the base and the top plate 110. Furthermore, a shock-absorbing element 240 can be disposed on the outer periphery of the first driving member 210, the height of which is equal to the distance between the base and the top plate 110, so that the top and bottom ends of the shock-absorbing element 240 abut against the top plate 110 and the base, respectively.

[0081] like Figure 2 Based on the design of the first driving member 210 having a shock-absorbing element 240 on its outer periphery, in one embodiment of the present invention, a plurality of shock-absorbing elements 240 may be provided on the outer periphery of the first driving member 210, and these shock-absorbing elements 240 may be evenly arranged at circumferential intervals along the first driving member 210.

[0082] Based on the design of the first driving member 210 having a shock-absorbing element 240 on its outer periphery, in one embodiment of the present invention, the shock-absorbing element 240 can be a rubber column.

[0083] In one embodiment of the present invention, the number of damping elements 240 may be 2-8, such as 2, 4, 6, etc. In some embodiments, the number of damping elements 240 may be less than 2 or more than 8, such as 1, 9, etc., and is not limited thereto.

[0084] In one embodiment of the present invention, the abrasive particles can be a composition comprising at least two types of abrasive particles, wherein the different types of abrasive particles have different particle sizes and / or shapes. In other words, for any two abrasive particles included in the composition, they can have the same shape but different particle sizes, or they can have approximately the same particle size but different shapes, or they can have both different particle sizes and shapes.

[0085] In one embodiment of the present invention, the composition consisting of at least two types of abrasive particles may have a mass percentage of 30% to 70% in the sample solution, such as 30%, 40%, 55%, 70%, etc. In some embodiments, the mass percentage of the composition in the sample solution may be less than 30% or greater than 70%, such as 25%, 75%, etc., and is not limited thereto.

[0086] In one embodiment of the present invention, the composition may specifically include a first abrasive particle and a second abrasive particle, wherein the particle size of the first abrasive particle is smaller than the particle size of the second abrasive particle.

[0087] In one embodiment of the present invention, the particle size of the first abrasive particle can be 0.1 mm to 0.45 mm, for example, 0.1 mm, 0.2 mm, 0.3 mm, 0.45 mm, etc. In some embodiments, the particle size of the first abrasive particle can also be less than 0.1 mm or greater than 0.45 mm, for example, 0.05 mm, 0.5 mm, etc., and is not limited thereto.

[0088] In one embodiment of the present invention, the particle size of the second abrasive particle can be 0.2 mm to 0.65 mm, for example, 0.2 mm, 0.3 mm, 0.5 mm, 0.65 mm, etc. In some embodiments, the particle size of the second abrasive particle can also be less than 0.2 mm or greater than 0.65 mm, for example, 0.15 mm, 0.7 mm, etc., and is not limited thereto.

[0089] In one embodiment of the present invention, the particle size of the first abrasive particle is 0.35 mm, and the particle size of the second abrasive particle is 0.55 mm.

[0090] In one embodiment of the present invention, the mass percentage of the first grinding particles in the sample solution can be 6% to 42%, for example, 6%, 10%, 25%, 42%, etc. In some embodiments, the mass percentage of the first grinding particles in the sample solution can also be less than 6% or greater than 42%, for example, 5%, 45%, etc., and is not limited thereto.

[0091] In one embodiment of the present invention, the mass percentage of the second grinding particles in the sample solution can be 12% to 56%, for example, 12%, 25%, 40%, 56%, etc. In some embodiments, the mass percentage of the second grinding particles in the sample solution can also be less than 12% or greater than 56%, for example, 11%, 60%, etc., and is not limited thereto.

[0092] In one embodiment of the present invention, the mass percentage of the first grinding particles in the sample solution can be 28%, and the mass percentage of the second grinding particles in the sample solution can be 50%.

[0093] In one embodiment of the present invention, the density of the first grinding particles can be 1 g / cm³. 3 ~3g / cm 3 For example, 1g / cm 3 1.5g / cm 3 2g / cm 3 3g / cm 3 In some embodiments, the density of the first abrasive particles may also be less than 1 g / cm³. 3 It may be greater than 3g / cm 3 For example, 0.8 g / cm³ 3 3.5g / cm 3 And so on, without being limited to this.

[0094] In one embodiment of the present invention, the density of the second grinding particles can be 1 g / cm³. 3 ~3g / cm 3 For example, 1g / cm 3 1.5g / cm 3 2g / cm 3 3g / cm 3 In some embodiments, the density of the second grinding particles may also be less than 1 g / cm³. 3 It may be greater than 3g / cm 3 For example, 0.8 g / cm³ 3 3.5g / cm 3 And so on, but not limited to this. In addition, the density of the first abrasive particle and the density of the second abrasive particle may be, but are not limited to, equal.

[0095] In one embodiment of the present invention, the density of the first abrasive particles is 1.6 g / cm³. 3 The density of the second batch of particles is 2.3 g / cm³. 3 .

[0096] In one embodiment of the present invention, the volume percentage of the first abrasive particles in the composition can be 20% to 60%, for example, 20%, 40%, 45%, 60%, etc. In some embodiments, the volume percentage of the first abrasive particles in the composition can also be less than 20%, or greater than 60%, for example, 15%, 65%, etc., and is not limited thereto.

[0097] In one embodiment of the present invention, the volume percentage of the second abrasive particles in the composition can be 40% to 80%, for example, 40%, 50%, 65%, 80%, etc. In some embodiments, the volume percentage of the second abrasive particles in the composition can also be less than 40%, or greater than 80%, for example, 35%, 85%, etc., and is not limited thereto.

[0098] In one embodiment of the present invention, the volume percentage of the first abrasive particles in the composition may be 55%, and the volume percentage of the second abrasive particles in the composition may be 45%.

[0099] Based on the design of the composition including the first abrasive particle and the second abrasive particle, in the first embodiment of the present invention, the composition may further include a third abrasive particle, the particle size of which is not equal to the particle size of the first abrasive particle and the second abrasive particle.

[0100] In one embodiment of the present invention, the particle size of the third abrasive particle can be 0.1 mm to 1 mm, for example, 0.1 mm, 0.2 mm, 0.6 mm, 1 mm, etc. In some embodiments, the particle size of the third abrasive particle can also be less than 0.1 mm or greater than 1 mm, for example, 0.09 mm, 1.05 mm, etc., and is not limited thereto.

[0101] In one embodiment of the present invention, the first abrasive particle has a particle size of 0.25 mm, the second abrasive particle has a particle size of 0.4 mm, and the third abrasive particle has a particle size of 0.75 mm.

[0102] In one embodiment of the present invention, the mass percentage of the third grinding particles in the sample solution can be 8% to 35%, for example, 8%, 15%, 25%, 35%, etc. In some embodiments, the mass percentage of the third grinding particles in the sample solution can also be less than 8% or greater than 35%, for example, 7%, 36%, etc., and is not limited thereto.

[0103] In one embodiment of the present invention, the mass percentage of the first grinding particles in the sample solution can be 32%, the mass percentage of the second grinding particles in the sample solution can be 38%, and the mass percentage of the second grinding particles in the sample solution can be 30%.

[0104] In one embodiment of the present invention, the density of the third grinding particle can be 1 g / cm³. 3 ~3g / cm 3 For example, 1g / cm 3 1.5g / cm 3 2g / cm 3 3g / cm 3 In some embodiments, the density of the third abrasive particles may also be less than 1 g / cm³. 3 It may be greater than 3g / cm 3 For example, 0.8 g / cm³ 3 3.5g / cm 3 And so on, but not limited to this. In addition, the density of the third abrasive particle may be equal to, but is not limited to, the density of the first and second abrasive particles.

[0105] In one embodiment of the present invention, the density of the first abrasive particles is 1.8 g / cm³. 3 The density of the second batch of particles is 2.2 g / cm³. 3 The density of the third grinding particle is 2.8 g / cm³. 3 .

[0106] In one embodiment of the present invention, the shape of the grinding particles can be spherical, ellipsoidal, egg-shaped, or other irregular shapes.

[0107] In one embodiment of the present invention, the surface of the abrasive particles may have depressions, and the number of depressions may be one or more.

[0108] In one embodiment of the present invention, the surface of the abrasive particles may have protrusions, and the number of protrusions may be one or more.

[0109] In one embodiment of the invention, the abrasive particles may be made of ceramic. In some embodiments, the abrasive particles may also be made of plastic or glass, and are not limited thereto.

[0110] In one embodiment of the present invention, the surface of the abrasive particles may be coated with a silicon-based shell.

[0111] It should be noted that the biological sample lysis apparatus shown in the accompanying drawings and described in this specification are merely a few examples among many lysis apparatuses capable of employing the principles of the present invention. It should be clearly understood that the principles of the present invention are by no means limited to any detail or component of the biological sample lysis apparatus shown in the accompanying drawings or described in this specification.

[0112] Based on the detailed description of several exemplary embodiments of the biological sample lysis device proposed in this invention above, an exemplary embodiment of the nucleic acid detection device proposed in this invention will be described below.

[0113] In one embodiment of the present invention, the nucleic acid detection device proposed by the present invention includes the biological sample lysis device proposed by the present invention and described in detail in the above embodiments.

[0114] It should be noted that the nucleic acid detection devices shown in the accompanying drawings and described in this specification are merely a few examples among many nucleic acid detection devices capable of employing the principles of the present invention. It should be clearly understood that the principles of the present invention are by no means limited to any detail or component of the nucleic acid detection devices shown in the accompanying drawings or described in this specification.

[0115] In summary, the pyrolysis tube 230 proposed in this invention, by designing a turbulence-inducing structure 233 protruding from the inner wall of the tube body 231, can improve the pyrolysis efficiency and make the pyrolysis more complete.

[0116] Furthermore, the biological sample lysis device proposed in this invention includes a support 100, a vortex mixing unit 200, and a reset assembly 300. The vortex mixing unit 200 is disposed on the support 100, and each vortex mixing unit 200 includes a clamping seat 220 and a lysis tube 230. The clamping seat 220 is rotatably disposed on the support 100. The lysis tube 230 is disposed on the clamping seat 220. The reset assembly 300 is disposed on the support 100 and is used to reset the rotated clamping seat 220 to its initial position before rotation. Through the above design, the biological sample lysis device proposed in this invention can quickly and actively reset the lysis tube 230 through the reset assembly 300, which helps to reduce operation steps, improve the automation level of the equipment, and achieve rapid and accurate detection functions.

[0117] The foregoing has described and / or illustrated exemplary embodiments of the lysis tube, biological sample lysis device, and nucleic acid detection device proposed in this invention. However, the embodiments of this invention are not limited to the specific embodiments described herein; rather, components and / or steps of each embodiment may be used independently and separately from other components and / or steps described herein. Each component and / or step of one embodiment may also be used in combination with other components and / or steps of other embodiments. In describing the elements / components / etc. described and / or illustrated herein, the terms "a," "an," and "the above" are used to indicate the presence of one or more elements / components / etc. The terms "comprising," "including," and "having" are used to indicate an open-ended inclusion and mean that additional elements / components / etc. may exist in addition to those listed. Furthermore, the terms "first" and "second," etc., in the claims and specification are used only as illustrative marks and are not intended to limit the numerical scope of the subject matter.

[0118] Although the lysis tube, biosample lysis device and nucleic acid detection device proposed in this invention have been described according to different specific embodiments, those skilled in the art will recognize that modifications can be made to the implementation of this invention within the spirit and scope of the claims.

Claims

1. A biological sample lysis tube, comprising: tube body; as well as A turbulence-disrupting structure is disposed on the inner wall of the tube body and protrudes relative to the inner wall; The length of the top end of the turbulence structure along the radial direction of the tube body is greater than the length of its bottom end along the radial direction of the tube body; The tube body has an opening at the top, and the pyrolysis tube also includes a cap for sealing the opening; wherein the cap is provided with an inner membrane that seals the lumen of the tube body, and both the cap and the inner membrane have cracks to allow the pipette tip to pass through.

2. The biological sample lysis tube according to claim 1, wherein, The pyrolysis tube includes multiple flow-disrupting structures, which are arranged at intervals along the circumference of the tube body.

3. The biological sample lysis tube according to claim 1, wherein: The turbulence-disrupting structure has a triangular, trapezoidal, or stepped shape in its axial cross-section along the tube body.

4. The biological sample lysis tube according to claim 1, wherein: The surface of the turbulence structure has protrusions; and / or Along the axial direction of the pipe body, the height of the turbulence-inducing structure accounts for 30% to 95% of the total height of the pipe body; and / or Along the radial direction of the tube body, the length of the turbulence-disrupting structure accounts for 15% to 40% of the inner diameter of the tube body; and / or Along the circumferential direction of the tube body, the width of the turbulence-disrupting structure accounts for 5% to 40% of the inner circumference of the tube body; and / or The distance between the bottom end of the turbulence-inducing structure and the bottom end of the tube body accounts for 3% to 40% of the height of the tube body; and / or The distance between the top of the turbulence-inducing structure and the top of the tube body accounts for 10% to 50% of the height of the tube body; and / or The turbulence-disrupting structure is seamlessly connected to the inner wall of the tube.

5. A biological sample lysis device, comprising: support; as well as At least one vortex mixing unit is disposed on the support, and each vortex mixing unit includes: The clamping seat is rotatably mounted on the bracket by a first driving member; and The biological sample lysis tube according to any one of claims 1 to 4 is disposed in the clamping seat and is used to hold the abrasive particles and sample liquid.

6. The biological sample lysis device according to claim 5, wherein, The biological sample lysis device also includes: A reset component, disposed on the bracket, is used to reset the rotated clamping seat to its initial position before rotation.

7. The biological sample lysis device according to claim 6, wherein, The clamping seat is provided with a first magnetic element; wherein, the reset assembly includes: A second magnetic element is movably disposed on the bracket and located on the side of the clamping seat where the first magnetic element is disposed; and The second driving component is disposed on the bracket and is connected to the second magnetic component in a transmission manner; The reset component is configured such that when the clamping seat finishes rotating, the second magnetic component is driven by the second driving component to approach the clamping seat, thereby resetting the clamping seat.

8. The biological sample lysis apparatus according to claim 7, wherein, The reset component further includes: A fixing seat is fixed to the bracket and located on the side of the clamping seat where the first magnetic element is provided; The second magnetic component has a guide rod connected to the side facing away from the clamping seat, and the guide rod is movably inserted through the fixed seat; The second driving component is disposed on the fixed base.

9. The biological sample lysis device according to claim 5, wherein, The output end of the first driving member includes an eccentric shaft, which is connected to the clamping seat via a flexible connector.

10. The biological sample lysis apparatus according to claim 5, wherein, The clamping seat includes a plurality of jaws, which are arranged at intervals along the circumference of the clamping seat, and are used to clamp the pyrolysis tube.

11. The biological sample lysis apparatus according to claim 5, wherein, The bracket includes a top plate, the clamping seat is rotatably disposed on the top surface of the top plate, the first driving member is disposed below the top plate, and the output end of the first driving member passes through the top plate and is connected to the clamping seat.

12. The biological sample lysis apparatus according to claim 11, wherein, The bracket is mounted on a base, and the top plate is located above the base. The first driving member is disposed between the base and the top plate. A shock-absorbing element is disposed on the outer periphery of the first driving member. The height of the shock-absorbing element is equal to the distance between the base and the top plate, so that the top and bottom ends of the shock-absorbing element abut against the top plate and the base, respectively.

13. The biological sample lysis apparatus according to claim 12, wherein: The first driving member has a plurality of damping elements disposed on its outer periphery, the plurality of damping elements being evenly spaced along the circumferential direction of the first driving member; and / or The damping element is a rubber column.

14. The biological sample lysis apparatus according to any one of claims 5 to 13, wherein, The abrasive particles are a composition, and the composition includes at least two abrasive particles with different particle sizes and / or shapes.

15. A nucleic acid detection device, comprising the biological sample lysis device according to any one of claims 5 to 14.

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