Nucleic acid detection card box and nucleic acid detection method

Abstract

The application provides a nucleic acid detection card box and a nucleic acid detection method, which comprise sample cavities, pipetting cavities and reagent cavities arranged at intervals along the length direction of the box body; a reaction tube provided with a reaction cavity; a control valve accommodated in a valve cavity and provided with a plurality of independent pipetting flow channels, the pipetting flow channels being used for connecting one of the sample cavities, the reagent cavities and the reaction cavity with the pipetting cavity; and a pipetting driving element arranged in the pipetting cavity and capable of ascending and descending in the pipetting cavity to form positive pressure or negative pressure in the pipetting cavity. The application integrates a plurality of pipetting flow channels in the control valve, simplifies the nucleic acid detection structure, does not need to arrange other flow channel structures, and can realize the change of the flow channel by driving the control valve to rotate, realizes the miniaturization of the nucleic acid detection card box, realizes liquid transfer and mixing by cooperating with the ascending and descending of the pipetting driving element, and can ensure that the nucleic acid detection card box always maintains a fully-closed detection state during the pipetting and mixing process.

Description

Technical Field

[0001] This invention belongs to the field of nucleic acid detection technology, and in particular relates to a nucleic acid detection cartridge and a nucleic acid detection method. Background Technology

[0002] PCR (Polymerase Chain Reaction) is a molecular biology technique that amplifies specific DNA (deoxyribonucleic acid) sequences in vitro. Due to its high specificity, high sensitivity, low purity requirements, and simplicity and speed, PCR is widely used in nucleic acid detection and analysis. The main steps in sample detection include nucleic acid extraction, purification, and PCR detection.

[0003] To achieve point-of-care testing (POCT) and improve testing accuracy, some fully automated testing instruments have been launched on the market. These instruments require various reagents to be pre-loaded into the kit, and the mixing and transfer of the reagents and samples within the kit are performed to achieve nucleic acid extraction and purification.

[0004] However, controlling the pipetting of reagents within the kit is difficult and complex, requiring the addition of pipetting pumps and related mechanisms to the instrument to accurately control reagent flow. This increases manufacturing difficulty and cost, and results in large, bulky instruments that are inconvenient to carry. Furthermore, the procedure remains consistent with traditional manual aspiration and transfer methods, which is time-consuming, and results cannot be released immediately, hindering widespread adoption at the grassroots level. Additionally, the external aspiration and transfer method cannot achieve fully enclosed testing, meaning nucleic acid testing still carries a high risk of infection. Summary of the Invention

[0005] The main objective of this invention is to propose a nucleic acid detection cartridge and a nucleic acid detection method, aiming to solve the technical problems of large volume and easy contamination of existing nucleic acid detection cartridges.

[0006] To achieve the above objectives, the present invention provides a nucleic acid detection cartridge comprising: a cartridge body, including a cavity group and a valve body cavity located below the cavity group, the cavity group including a sample cavity, a pipetting cavity and a reagent cavity spaced apart along the length of the cartridge body, the valve body cavity extending along the length of the cartridge body; a reaction tube having a reaction cavity; a control valve, housed within the valve body cavity and having multiple independent pipetting channels, the pipetting channels being used to connect one of the sample cavity, the reagent cavity, the reaction cavity and the pipetting cavity; and a pipetting drive, disposed within the pipetting cavity and capable of moving up and down within the pipetting cavity to create positive or negative pressure within the pipetting cavity.

[0007] In this embodiment of the invention, each of the pipetting channels is provided with a temporary storage port and a pipetting port. The pipetting port is used to connect to one of the sample chamber, the reagent chamber and the reaction chamber. The temporary storage port is used to connect to the pipetting chamber. The control valve can rotate relative to the housing along its own axis. The plurality of temporary storage ports are arranged circumferentially around the control valve.

[0008] In this embodiment of the invention, a first sealing ring is provided on both sides of the pipetting connection port along the axial direction of the control valve, and a second sealing ring is provided on both sides of the temporary storage connection port along the axial direction of the control valve. The first sealing ring and the second sealing ring are both sealed and fitted with the cavity wall of the valve body cavity.

[0009] In this embodiment of the invention, one end of the control valve is provided with a flow channel opening, and all the pipetting channels extend from the flow channel opening along the length of the box. A sealing plug is provided between one of the temporary storage connection port and the pipetting connection port and the flow channel opening, so that the pipetting channels form staggered pipetting sections.

[0010] In this embodiment of the invention, the control valve includes: a valve body, which is housed in the valve body cavity, and the liquid transfer channels are all opened in the valve body; a rotating head, which is connected to the operating end of the valve body, the rotating head partially extending out of the valve body cavity, and an operating handle is provided on the side of the rotating head away from the valve body.

[0011] In this embodiment of the invention, the housing is provided with a clearance space for accommodating the drive device at the position corresponding to the operating handle, and the control valve is used to rotate under the drive of the drive device.

[0012] In this embodiment of the invention, the control valve further includes a buckle, which is connected to the side of the valve body away from the rotating head, and the housing is provided with a limiting groove that cooperates with the buckle.

[0013] In this embodiment of the invention, the box body is further provided with an ultrasonic cavity for the ultrasonic device to extend into, the ultrasonic cavity being located below the sample cavity; and / or, the box body is further provided with a waste liquid cavity, and the reaction tube is provided with a drain channel communicating with the waste liquid cavity.

[0014] The present invention also proposes a nucleic acid detection method, applied to the nucleic acid detection cartridge described above, the nucleic acid detection method comprising:

[0015] Add the sample to be tested into the sample chamber and the test reagent into the reagent chamber;

[0016] The drive control valve and the pipetting drive work together to perform the operation of acquiring the liquid to be tested, so that the reaction chamber is filled with the liquid to be tested.

[0017] In this embodiment of the invention, the drive control valve and the pipetting drive jointly perform the test liquid acquisition operation to obtain the test liquid, including:

[0018] The drive control valve performs a first rotation operation to connect the sample chamber and the pipetting chamber through the pipetting channel;

[0019] The driving component performs an upward motion to transfer liquid from the sample chamber to the pipetting chamber through the pipetting channel.

[0020] The drive control valve performs a second rotation operation to connect the reagent chamber and the pipetting chamber through the pipetting channel;

[0021] The driving component of the pipetting device performs a downward motion to transfer the liquid in the pipetting chamber to the reagent chamber through the pipetting channel;

[0022] The drive unit performs a vertical reciprocating operation until the liquid to be tested is obtained;

[0023] The liquid to be tested is transferred to the pipetting chamber by the movement of the pipetting drive component;

[0024] The drive control valve performs a third rotation operation to connect the reaction chamber and the pipetting chamber through the pipetting channel;

[0025] The driving mechanism performs a downward motion to transfer the liquid in the pipetting chamber through the pipetting channel and the pipetting chamber to the reaction chamber.

[0026] Through the above technical solution, the nucleic acid detection kit provided in this embodiment of the invention has the following beneficial effects:

[0027] For nucleic acid testing, the test reagent is first placed in the reagent chamber, and the sample is placed in the sample chamber. The flow path is changed by rotating the control valve. First, rotating the control valve to the first position connects one of the pipetting channels to the sample chamber and the pipetting chamber, which drives the pipetting actuator upward, creating negative pressure in the pipetting chamber. Liquid from the sample chamber can then flow into the pipetting chamber through the negative pressure. Rotating the control valve to the second position connects the other pipetting channel to the pipetting chamber and the reagent chamber, which drives the pipetting actuator downward, creating positive pressure in the pipetting chamber. Liquid in the pipetting chamber flows into the pipetting chamber under this positive pressure. Under the action of the pipetting mechanism, the liquid can be transferred to the reagent chamber through the pipetting channel and mixed with the detection reagent in the reagent chamber. The liquid can be moved back and forth between the pipetting chamber, the pipetting channel, and the reagent chamber by the reciprocating lifting and lowering pipetting drive, ensuring thorough mixing. Then, the pipetting drive can be driven to rise, transferring the mixed test solution to the pipetting chamber. The control valve is then rotated to the third position, connecting the reaction chamber and the pipetting chamber through another pipetting channel. The pipetting drive is then driven to descend, allowing the liquid in the pipetting chamber to enter the reaction chamber under positive pressure through the pipetting channel. The reaction tube can then be inserted into the PCR module of the nucleic acid detection device for nucleic acid detection. This invention's nucleic acid detection cartridge simplifies the nucleic acid detection structure by integrating multiple pipetting channels into the control valve. No other channel structures are needed; channel changes are achieved simply by rotating the control valve, resulting in miniaturization of the nucleic acid detection cartridge. Combined with the lifting and lowering of the pipetting drive to achieve liquid transfer and mixing, it ensures that the nucleic acid detection cartridge remains fully enclosed during the pipetting and mixing process, preventing contamination of the internal environment.

[0028] Other features and advantages of the present invention will be described in detail in the following detailed description section. Attached Figure Description

[0029] The accompanying drawings are provided to illustrate the invention and form part of the specification. They are used together with the following detailed description to explain the invention, but do not constitute a limitation thereof. In the drawings:

[0030] Figure 1 This is a schematic diagram of the structure of a nucleic acid detection cartridge according to an embodiment of the present invention;

[0031] Figure 2 This is a schematic cross-sectional view of a nucleic acid detection cartridge according to an embodiment of the present invention;

[0032] Figure 3 This is a schematic diagram of the structure of a nucleic acid detection cartridge according to another embodiment of the present invention;

[0033] Figure 4 This is a schematic diagram of the cross-sectional structure of a nucleic acid detection cartridge according to another embodiment of the present invention;

[0034] Figure 5 This is a partial cross-sectional structural diagram of a nucleic acid detection cartridge according to another embodiment of the present invention;

[0035] Figure 6 This is a schematic diagram of the control valve structure of a nucleic acid detection cartridge according to an embodiment of the present invention;

[0036] Figure 7 This is a schematic diagram of the cross-sectional structure of the control valve of a nucleic acid detection cartridge according to an embodiment of the present invention;

[0037] Figure 8 This is a perspective view of the control valve of a nucleic acid detection cartridge according to an embodiment of the present invention.

[0038] Explanation of reference numerals in the attached figures

[0039] Label Name Label Name

[0040] 100 Nucleic Acid Detection Kits 31a First Flow Channel

[0041] 1. Box 31b Second flow channel

[0042] 11 Sample Chamber 31c Third Flow Channel

[0043] 12 Pipetting chamber; 32 Temporary storage connection port

[0044] 13 Reagent chamber; 33 Pipette connection port

[0045] 14 Inlet channel; 34 Channel opening

[0046] 15 Valve body cavity 35 Sealing plug

[0047] 16 Waste liquid chamber; 36 Valve body

[0048] 17 Clearance space 37 Rotating head

[0049] 18. Ultrasonic cavity 371. Operating handle.

[0050] 19 Sealing groove 38 Undercut

[0051] 10. Mounting slot 4. Pipetting drive unit

[0052] 2. Reaction tube 5. First sealing ring

[0053] 21 Drainage channel 6 Second sealing ring

[0054] 3 Control valve 7 Filter plug

[0055] 31 Pipettes 8 Sample Cap Detailed Implementation

[0056] The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0057] The nucleic acid detection cartridge according to the present invention is described below with reference to the accompanying drawings.

[0058] like Figures 1 to 8 As shown, in an embodiment of the present invention, the nucleic acid detection cartridge 100 includes a cartridge body 1, a reaction tube 2, a control valve 3, and a pipetting drive 4. The cartridge body 1 includes a cavity group and a valve body cavity 15 located below the cavity group. The cavity group includes a sample cavity 11, a pipetting cavity 12, and a reagent cavity 13 spaced apart along the length of the cartridge body 1. The valve body cavity 15 extends along the length of the cartridge body. The reaction tube 2 is provided with a reaction cavity. The control valve 3 is housed in the valve body cavity 15 and has multiple independent pipetting channels 31. The pipetting channels 31 extend along the length of the cartridge body and are used to connect one of the sample cavity 11, the reagent cavity 13, the reaction cavity, and the pipetting cavity 12. The pipetting drive 4 is disposed in the pipetting cavity 12 and can move up and down within the pipetting cavity 12, creating positive or negative pressure within the pipetting cavity 12. The pipetting drive 4 can be a pipetting piston adapted to the pipetting drive 4.

[0059] In one embodiment, the length direction of the box and the axial direction of the control valve 3 are aligned, both being left-right. There is one reagent chamber 13. The sample chamber 11, pipetting chamber 12, and reagent chamber 13 are arranged sequentially along the left-right direction, and all three extend vertically. The box body 1 may have an L-shaped inlet channel 14. The pipetting chamber 12 can be connected to the reaction chamber sequentially through the pipetting channel 31 and the inlet channel 14. The reaction tube 2 is located on the side near the inlet channel 14. The box body 1 may have a mounting groove 10 for sealing connection with the reaction tube 2. There are three pipetting channels 31: a first channel 31a, a second channel 31b, and a third channel 31c. The first channel 31a connects the sample chamber 11 and the pipetting chamber 12; the second channel 31b connects the pipetting chamber 12 and the reagent chamber 13; and the third channel 31c connects the pipetting chamber 12 and the inlet channel 14. In other embodiments, the number of reagent chambers 13 can be set according to actual usage requirements. The number of pipetting channels 31 is consistent with the sum of the number of sample chambers 11, pipetting chambers 12, reagent chambers 13, and inlet channels 14. The nucleic acid detection cartridge 100 may also include a sample cap 8 for detachable connection with the cartridge body 1, the sample cap 8 for sealing the sample chamber 11. The control valve 3 is rotatably mounted in the valve body cavity 15, and can be manually or driven by a drive device to rotate around its own axis. During rotation, the flow channel can be changed, and the pipetting drive 4 can then move up and down in the pipetting chamber 12 manually or driven by a drive device.

[0060] When using the nucleic acid detection cartridge 100 in this embodiment for sample detection, the detection reagent can be placed into the reagent chamber 13 first, and the sample into the sample chamber 11. The flow channel can be changed by rotating the drive control valve 3. First, the control valve 3 can be rotated to the first position, so that one of the pipetting channels 31 connects the sample chamber 11 and the pipetting chamber 12. This can drive the pipetting drive 4 to move upward, creating a negative pressure in the pipetting chamber 12. Under the action of negative pressure, the liquid in the sample chamber 11 can enter the pipetting chamber 12 through the pipetting channel 31. When the drive control valve 3 is rotated to the second position, so that the other pipetting channel 31 connects the pipetting chamber 12 and the reagent chamber 13, the pipetting drive 4 can be driven to move downward, creating a positive pressure in the pipetting chamber 12. Under positive pressure, the liquid in pipette 12 can be transferred through pipette channel 31 to reagent chamber 13 and mixed with the detection reagent in reagent chamber 13. The liquid can be made to flow back and forth between pipette chamber 12, pipette channel 31 and reagent chamber 13 by reciprocating lifting and lowering pipette drive 4. After the liquid is fully mixed, pipette drive 4 can be driven to move upward to transfer the mixed test solution to pipette chamber 12. Drive control valve 3 to rotate to the third position, so that another pipette channel 31 connects the reaction chamber and pipette chamber 12. Drive pipette drive 4 to move downward. The liquid in pipette chamber 12 can enter the reaction chamber through pipette channel 31 under positive pressure. The reaction tube 2 can enter the PCR module of nucleic acid detection equipment for amplification and optical detection. In this embodiment, the nucleic acid detection cartridge 100 simplifies the nucleic acid detection structure by integrating multiple pipetting channels 31 into the control valve 3. No other channel structure is required; the channel can be changed simply by driving the control valve 3 to rotate. This achieves miniaturization of the nucleic acid detection cartridge 100. Combined with the lifting and lowering of the pipetting drive 4, liquid transfer and mixing are achieved, ensuring that the nucleic acid detection cartridge 100 remains in a fully enclosed state during the pipetting and mixing process, thus preventing the internal environment of the nucleic acid detection cartridge 100 from being contaminated.

[0061] Understandably, to ensure smooth liquid transfer, the bottom of the sample chamber 11, pipetting chamber 12 and reagent chamber 13 are all provided with vertical flow channels, and the valve body chamber 15 is connected to the lower end of the vertical flow channels.

[0062] like Figure 2 , Figure 4 , Figure 6As shown, each pipetting channel 31 is equipped with a temporary storage port 32 and a pipetting port 33. The pipetting port 33 is used to connect one of the sample chamber 11, reagent chamber 13, and reaction chamber. The temporary storage port 32 is used to connect the pipetting chamber 12. The control valve 3 can rotate relative to the housing 1 along its own axis, and the pipetting channel 31 connects one of the sample chamber 11, reagent chamber 13, reaction chamber, and pipetting chamber 12. Multiple temporary storage ports 32 are arranged circumferentially around the control valve 3. The control valve 3 is a rotary valve that can rotate along its own axis. By rotating the control valve 3, the pipetting channel 31 can connect one of the sample chamber 11, reagent chamber 13, reaction chamber, and pipetting chamber 12. The structure is simple and easy to operate. When there are three pipetting channels 31, the three temporary connection ports 32 are arranged on the same circumference around the control valve 3, which can ensure the alignment and communication between the pipetting chamber 12 and the pipetting channel 31, and the structure is compact, which can realize the miniaturization of the control valve 3.

[0063] In one embodiment, a first sealing ring 5 is provided on both sides of the pipetting connection 33 along the axial direction of the control valve 3, and a second sealing ring 6 is provided on both sides of the temporary storage connection 32 along the axial direction of the control valve 3. Both the first sealing ring 5 and the second sealing ring 6 are sealed to the cavity wall of the valve body cavity 15. In this embodiment, the first sealing ring 5 is provided at both ends of the pipetting connection 33. The first sealing ring 5 protrudes outward relative to the pipetting connection 33 and can be press-fitted to the cavity wall of the valve body cavity 15 to achieve a sealed fit between the control valve 3 and the valve body cavity 15, preventing leakage during the rotation of the control valve 3. In other embodiments, multiple first sealing rings 5 ​​are provided on both sides of the pipetting connection 33, and the multiple first sealing rings 5 ​​can be spaced apart in the left and right direction. Multiple second sealing rings 6 are provided on both sides of the temporary storage connection 32, and the second sealing rings 6 can be spaced apart in the left and right direction. In this embodiment, by providing a first sealing ring 5 and a second sealing ring 6 on the control valve 3, the sealing performance of the nucleic acid detection cartridge 100 can be further improved. The first sealing ring 5 and the second sealing ring 6 can be elastic elements.

[0064] Specifically, one end of the control valve 3 has a flow channel opening 34, and all pipetting channels 31 extend from the flow channel opening 34 along the length of the container. A sealing plug 35 is provided between one of the temporary storage connection port 32 and the pipetting connection port 33 and the flow channel opening 34, so that the pipetting channels 31 form staggered pipetting sections. In this embodiment, all pipetting channels 31 open from one end of the control valve 3, which facilitates the production of the control valve 3 and the opening of the flow channels. By placing the sealing plugs 35 in different positions in each pipetting channel 31, staggered pipetting sections are formed between the pipetting channels 31. The sealing plugs 35 can isolate the pipetting channels 31 into idle sections and pipetting sections. The control valve 3 in this embodiment is not only simple in structure and easy to produce, but also reduces the production cost of the control valve 3.

[0065] It should be noted that the control valve 3 includes a valve body 36 and a rotating head 37. The valve body 36 is housed in the valve body cavity 15, and the pipetting channels 31 are all opened in the valve body 36. The rotating head 37 is connected to the operating end of the valve body 36, and part of the rotating head 37 extends out of the valve body cavity 15. An operating handle 371 is provided on the side of the rotating head 37 away from the valve body 36. Both the valve body 36 and the rotating head 37 are cylindrical and coaxially arranged. The operating handle 371 can be an elongated boss, and the extension direction of the operating handle 371 can extend radially along the rotating head 37. In this embodiment, the cross-sectional dimension of the rotating head 37 is larger than that of the valve body 36, which facilitates operation by the drive device or operator. In this embodiment, when the control valve 3 is inserted into the valve body cavity 15, the operating handle 371 protrudes out of the valve body cavity 15 and divides the rotating head 37 into two areas. The three flow channel openings 34 are all located on the same side of the operating handle 371, which can make the rotation angle of the control valve 3 smaller, thereby improving the efficiency of nucleic acid detection.

[0066] like Figure 1 and Figure 2 As shown, in one embodiment, the housing 1 has a clearance space 17 for accommodating the driving device at the position corresponding to the operating handle 371, and the control valve 3 is used to rotate under the drive of the driving device. In this embodiment, the housing 1 has a cross-section that is larger at the top and smaller at the bottom, and the reaction tube 2 and the rotating head 37 are both located on the same side of the housing 1. The clearance space 17 is located below the reaction tube 2, which enables the miniaturization of the nucleic acid detection cartridge 100 while also facilitating the connection between the driving device and the control valve 3. Figures 3 to 5 As shown, in another embodiment, the box 1 has a rectangular cross-section, and the reaction tube 2 and the rotating head 37 are respectively arranged on opposite sides of the box 1 along the length direction of the box 1, which can reserve enough cavity space for the cavity assembly.

[0067] like Figures 3 to 8 As shown, in one embodiment, the control valve 3 further includes a buckle 38, which connects to the side of the valve body 36 away from the rotating head 37. The housing 1 is provided with a limiting groove that cooperates with the buckle 38. In this embodiment, the valve body cavity 15 extends through the housing 1 in the left-right direction, ensuring that the control valve 3 has sufficient installation space. The limiting groove is provided at the end of the valve body cavity 15. In this embodiment, the buckle 38 is provided with a deformation notch and a frustum-shaped protrusion at the head, which facilitates the control valve 3 to extend into the limiting groove. The buckle 38 and the limiting groove cooperate in the left-right direction, which can prevent the control valve 3 from coming out of the cavity during rotation.

[0068] like Figure 2As shown, in another embodiment, the valve body cavity 15 does not penetrate the box body 1, and the length of the control valve 3 is less than the length of the box body 1. The box body 1 also has an ultrasonic cavity 18 for the ultrasonic equipment to extend into, and the ultrasonic cavity 18 is located below the sample cavity 11; in this embodiment, the ultrasonic cavity 18 is located at the bottom of the sample cavity 11, and the ultrasonic cavity 18 and the valve body cavity 15 are spaced apart in the left-right direction. The clearance space 17 and the sample cavity 11 are respectively located on both sides of the valve body cavity 15 in the left-right direction, which can further improve the structural compactness of the nucleic acid detection card cassette 100 and realize the miniaturization of the nucleic acid detection card cassette 100. Figure 2 and Figure 5 As shown, the housing 1 also has a waste liquid chamber 16, and the reaction tube 2 is provided with a drain channel 21 communicating with the waste liquid chamber 16. In this embodiment, the waste liquid chamber 16 is located between the reagent chamber 13 and the reaction tube 2. After the detection is completed in the reaction tube 2, the waste liquid in the reaction tube 2 can be discharged into the waste liquid chamber 16 through the drain channel 21. In this embodiment, the cross-section of the waste liquid chamber 16 can be L-shaped, and the drain channel 21 can be a straight line extending in the left and right direction. The top of both the waste liquid chamber 16 and the reagent chamber 13 is provided with a sealing groove 19 to accommodate the filter plug 7. In one embodiment, the cross-sectional dimensions of both the reagent chamber 13 and the waste liquid chamber 16 are smaller than the cross-sectional dimensions of the sealing groove 19, which facilitates the installation of the filter plug 7.

[0069] This invention also proposes a nucleic acid detection method, applied to the nucleic acid detection cartridge 100 as described above. The nucleic acid detection method includes:

[0070] Step S1: Add the sample to be tested into the sample chamber 11 and the test reagent into the reagent chamber 13;

[0071] Step S2: Seal the sample chamber 11 and the reagent chamber 13;

[0072] In step S3, the drive control valve 3 and the liquid transfer drive 4 work together to perform the liquid to be tested acquisition operation so that the reaction chamber is filled with the liquid to be tested.

[0073] In this embodiment, the control valve 3 can be rotated along its own axis by the control drive device, which in turn drives the pipetting drive 4 to move up and down. During the upward movement of the pipetting drive 4, a negative pressure is created within the pipetting chamber 12, allowing liquid to be transferred into the chamber. During the downward movement of the pipetting drive 4, a positive pressure is created within the pipetting chamber 12, allowing liquid to be expelled. In this embodiment, by rotating the control valve 3 in conjunction with the upward and downward movement of the pipetting drive 4, the sample and reagents can be mixed, and the solution to be tested can be obtained. The detection operation is simple and can greatly improve the efficiency of nucleic acid detection.

[0074] Specifically, in one embodiment, the drive control valve 3 and the pipetting drive 4 jointly perform the test liquid acquisition operation to obtain the test liquid, including:

[0075] Step S31: Drive the control valve 3 to perform the first rotation operation so that the sample chamber and the pipetting chamber 12 are connected through the pipetting channel 31;

[0076] Step S32: Drive the pipetting drive 4 to perform an upward motion operation so that the liquid in the sample chamber 11 is transferred to the pipetting chamber 12 through the pipetting channel 31;

[0077] Step S33: Drive the control valve 3 to perform a second rotation operation so that the reagent chamber 13 and the pipetting chamber 12 are connected through the pipetting channel 31;

[0078] Step S34: Drive the pipetting drive 4 to perform a downward motion operation so that the liquid in the pipetting chamber 12 is transferred to the reagent chamber 13 through the pipetting channel 31;

[0079] Step S35: Drive the pipetting drive 4 to perform a vertical reciprocating operation until the liquid to be tested is obtained;

[0080] Step S36: The liquid to be tested is transferred to the pipetting chamber 12 by the movement of the pipetting drive 4;

[0081] Step S37: Drive the control valve 3 to perform a third rotation operation so that the reaction chamber and the pipetting chamber 12 are connected through the pipetting channel 31;

[0082] Step S38: Drive the pipetting drive 4 to perform a downward motion operation so that the liquid in the pipetting chamber 12 is transferred to the reaction chamber through the pipetting channel 31 and the pipetting chamber 12.

[0083] When there are multiple reagent chambers 13, steps S34 and S35 can be used as a reagent mixing operation unit, and the operation steps of the reagent mixing operation unit can be rotated and executed according to the number of reagent chambers 13.

[0084] First, the test reagent is placed into the reagent chamber 13, and the sample is placed into the sample chamber 11. The flow path is changed by rotating the control valve 3. First, the control valve 3 is rotated to the first position, connecting one of the pipetting channels 31 to the sample chamber 11 and the pipetting chamber 12. This drives the pipetting actuator 4 to move upward, creating a negative pressure in the pipetting chamber 12. Under this negative pressure, the liquid in the sample chamber 11 can enter the pipetting chamber 12 through the pipetting channel 31. Then, the control valve 3 is rotated to the second position, connecting the other pipetting channel 31 to the pipetting chamber 12 and the reagent chamber 13. This drives the pipetting actuator 4 to move downward, creating a positive pressure in the pipetting chamber 12. The liquid in the pipetting chamber 12 then... Under positive pressure, the liquid can be transferred through the pipette channel 31 to the reagent chamber 13 and mixed with the detection reagent in the reagent chamber 13. The liquid can be made to flow back and forth in the pipette chamber 12, the pipette channel 31 and the reagent chamber 13 by the reciprocating lifting and lowering pipette drive 4. After the liquid is fully mixed, the pipette drive 4 can be driven to move upward to transfer the mixed test liquid to the pipette chamber 12. The control valve 3 is driven to perform the third rotation operation, so that the control valve 3 rotates to the third position, so that the pipette channel 31 connects the reaction chamber and the pipette chamber 12. The pipette drive 4 is driven to move downward. The liquid in the pipette chamber 12 can enter the reaction chamber through the pipette channel 31 under positive pressure. The reaction tube 2 can enter the PCR module of the nucleic acid detection equipment for amplification and optical detection.

[0085] In this embodiment, the pretreatment of the test solution can be achieved simply by rotating the control valve 3 in conjunction with the lifting and lowering of the pipetting drive 4. This method is easy to operate and control, and can greatly improve the efficiency and accuracy of nucleic acid testing. Specifically, the first, second, and third positions can be set according to actual usage requirements.

[0086] In the description of this invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0087] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0088] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0089] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A nucleic acid testing cartridge, characterized by, The nucleic acid test kit (100) includes: The box body (1) includes a cavity group and a valve body cavity (15) located below the cavity group. The cavity group includes a sample cavity (11), a pipetting cavity (12) and a reagent cavity (13) spaced apart along the length of the box body (1). The valve body cavity (15) extends along the length of the box body. The reaction tube (2) is equipped with a reaction chamber; The control valve (3) is housed in the valve body cavity (15) and has multiple independent pipetting channels (31). The pipetting channels (31) are used to connect one of the sample cavity (11), the reagent cavity (13), the reaction cavity and the pipetting cavity (12). A pipetting drive (4) is disposed in the pipetting chamber (12) and can move up and down in the pipetting chamber (12) to create a positive or negative pressure in the pipetting chamber (12).

2. The nucleic acid testing cartridge of claim 1, wherein, Each of the pipetting channels (31) is provided with a temporary storage port (32) and a pipetting port (33). The pipetting port (33) is used to connect one of the sample chamber (11), the reagent chamber (13) and the reaction chamber. The temporary storage port (32) is used to connect the pipetting chamber (12). The control valve (3) can rotate relative to the housing (1) along its own axis. The multiple temporary storage ports (32) are arranged circumferentially around the control valve (3).

3. The nucleic acid testing cartridge of claim 2, wherein, The pipetting connection port (33) is provided with a first sealing ring (5) on both sides along the axial direction of the control valve (3), and a second sealing ring (6) is provided on both sides along the axial direction of the temporary storage connection port (32). The first sealing ring (5) and the second sealing ring (6) are sealed and fitted with the cavity wall of the valve body cavity (15).

4. The nucleic acid testing cartridge of claim 2, wherein, One end of the control valve (3) is provided with a flow channel opening (34), and the pipetting channels (31) all extend from the flow channel opening (34) along the length of the box. A sealing plug (35) is provided between one of the temporary storage connection port (32) and the pipetting connection port (33) and the flow channel opening (34), so that the pipetting sections are staggered between the pipetting channels (31).

5. The nucleic acid detection cartridge according to any one of claims 1 to 4, characterized in that, The control valve (3) includes: The valve body (36) is housed in the valve body cavity (15), and the liquid transfer channels (31) are all opened in the valve body (36); A rotating head (37) is connected to the operating end of the valve body (36). The rotating head (37) extends out of the valve body cavity (15), and an operating handle (371) is provided on the side of the rotating head (37) away from the valve body (36).

6. The nucleic acid detection cartridge according to claim 5, characterized in that, The housing (1) is provided with a clearance space (17) for accommodating the drive device at the position corresponding to the operating handle (371), and the control valve (3) is used to rotate under the drive of the drive device.

7. The nucleic acid detection cartridge according to claim 5, characterized in that, The control valve (3) also includes a buckle (38), which is connected to the side of the valve body (36) away from the rotating head (37). The housing (1) is provided with a limiting groove that cooperates with the buckle (38).

8. The nucleic acid detection cartridge according to any one of claims 1 to 4, characterized in that, The box body (1) is also provided with an ultrasonic cavity (18) for the ultrasonic equipment to extend into, and the ultrasonic cavity (18) is located below the sample cavity (11); And / or, The box body (1) is also provided with a waste liquid chamber (16), and the reaction tube (2) is provided with a drain channel (21) that communicates with the waste liquid chamber (16).

9. A nucleic acid detection method, applied to the nucleic acid detection cartridge (100) according to any one of claims 1 to 8, characterized in that, The nucleic acid detection method includes: The sample to be tested is added to the sample chamber (11), and the test reagent is added to the reagent chamber (13); The drive control valve (3) and the liquid transfer drive (4) work together to perform the liquid acquisition operation so that the reaction chamber is filled with the liquid to be tested.

10. The nucleic acid detection method according to claim 9, characterized in that, The drive control valve (3) and the pipetting drive (4) jointly perform the test liquid acquisition operation to obtain the test liquid, including: The drive control valve (3) performs a first rotation operation to connect the sample chamber and the pipetting chamber (12) through the pipetting channel (31); The driving pipetting actuator (4) performs an upward motion operation to transfer the liquid in the sample chamber (11) to the pipetting chamber (12) through the pipetting channel (31); Drive the control valve (3) to perform a second rotation operation so that the reagent chamber (13) and the pipetting chamber (12) are connected through the pipetting channel (31); The driving pipetting actuator (4) performs a downward motion operation to transfer the liquid in the pipetting chamber (12) to the reagent chamber (13) through the pipetting channel (31); The pipetting drive (4) performs a vertical reciprocating operation until the liquid to be tested is obtained; The liquid to be tested is transferred to the pipetting chamber (12) by the movement of the pipetting drive (4); Drive the control valve (3) to perform a third rotation operation so that the reaction chamber and the pipetting chamber (12) are connected through the pipetting channel (31); The driving pipetting actuator (4) performs a downward motion operation to transfer the liquid in the pipetting chamber (12) to the reaction chamber through the pipetting channel (31) and the pipetting chamber (12).

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