A biofilm sensor and a method for detecting biotoxicity using the same.

By using Geobacterium, Acinetobacter, and Pseudomonas to prepare biofilm sensors and controlling the biofilm thickness, the problems of low detection sensitivity and high cost in existing technologies are solved, enabling rapid and sensitive detection of new pollutants, which is suitable for open environments.

CN116555085BActive Publication Date: 2026-05-26CHENGDU INSTITUTE OF BIOLOGY CHINESE ACADEMY OF SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHENGDU INSTITUTE OF BIOLOGY CHINESE ACADEMY OF SCIENCES
Filing Date
2023-04-12
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Among existing detection technologies, water biotoxicity methods based on aquatic animals and luminescent bacteria have low accuracy, and bioelectrochemical sensors are costly, have short lifespans and poor sustainability, making them difficult to use in open environments. Furthermore, excessively thick anode biofilms affect detection sensitivity and cannot effectively detect the biotoxicity of new pollutants.

Method used

Biofilm sensors were prepared using Geobacterium, Acinetobacter, and Pseudomonas. The thickness of the biofilm was adjusted by controlling the carbon source concentration. The biotoxicity of pollutants was detected by combining the current changes of the electrode biofilm. Thinner electroactive biofilms were prepared using acclimatization culture medium and culture medium with low carbon source concentration.

Benefits of technology

It enables rapid and sensitive detection of new pollutants, improves detection sensitivity, reduces costs, and is suitable for biotoxicity detection of new pollutants in open environments.

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Abstract

This invention belongs to the field of microbiology, specifically relating to a biofilm sensor and a method for detecting biotoxicity using it. The specific technical solution involves a bacterial preparation comprising *Geobacterium*, *Acinetobacter*, and *Pseudomonas*. A sensor is prepared using this bacterial preparation and used to detect the biotoxicity of pollutants. This invention provides a novel biofilm sensor that, based on the high electroactivity of *Geobacterium* and by limiting the amount of carbon source used during cultivation, ultimately achieves an electroactive biofilm with a thickness lower than that of existing technologies, and realizes extremely high detection sensitivity, possessing enormous potential for detection applications.
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