Electrically conductive pipette mounting adapter, sample tube opening and closing device, and sample automatic analysis system

Abstract

The present invention relates to an electrically conductive pipette mounting adapter, a sample tube opening and closing device, and a sample automatic analysis system, and more particularly to an electrically conductive pipette mounting adapter, a sample tube opening and closing device, and a sample automatic analysis system that integrally perform dispensing of a liquid sample, extraction of nucleic acid, amplification, and detection. A sample tube opening and closing device includes a housing (110) forming an internal space isolated from the outside, including a door portion (120) for transporting a multi-well plate (20) for biological samples having a plurality of sample tubes (10) for containing biological samples into and out of the internal space; and a sample tube opening and closing portion provided in the internal space apart from the multi-well plate (20) for biological samples, automatically opening and closing the sample tubes (10).

Description

Technical Field

[0001] This invention relates to an adapter for installing conductive pipettes, a sample tube opening and closing device, and an automated sample analysis system. More specifically, it relates to an adapter for installing conductive pipettes, a sample tube opening and closing device, and an automated sample analysis system that integrates the dispensing of liquid samples, extraction, amplification, and detection of nucleic acids. Background Technology

[0002] Gene amplification detection is an in vitro diagnostic testing (IVD) technique that amplifies a specific gene sequence to determine the presence or absence of the gene. It is applicable not only to the detection of pathogenic microorganisms and genotypes in various animals and plants, including humans, but also to food testing, GMO testing, and many other fields.

[0003] In order to accurately detect gene amplification in various biological samples, it is first necessary to obtain high-purity target nucleic acids by removing various reaction-inhibiting substances in the samples that hinder the gene amplification reaction.

[0004] After mixing the extracted target nucleic acid with the gene amplification solution to perform a gene amplification reaction, the DNA length of the corresponding gene amplification product is examined or the fluorescence generated in the gene amplification product is examined to complete the gene amplification detection.

[0005] More specifically, gene amplification detection is a method used to amplify target nucleic acids. Various methods have been developed, such as PCR, Nested PCR, RT / PCR, and isothermal nucleic acid amplification. It generally includes: a preparation step, in which the extracted target nucleic acid and gene amplification reaction solution are mixed to prepare the gene amplification reaction material; and a reaction step, in which the reaction is carried out.

[0006] According to traditional technology, the dispensing device for dispensing biological samples, the purification and extraction device for purifying and extracting target substances from the dispensed biological samples, and the amplification and detection device for amplifying and detecting the extracted target substances are separate from each other, and dispensing, extraction and amplification are performed independently.

[0007] In this situation, rapid detection is difficult due to the increased time required from dispensing to amplification detection, and there is also the risk of exposure to contaminants during transport.

[0008] In addition, regarding biological sample dispensing devices, traditional technology dispenses biological samples by supplying them through a tray while the sample tube containing the biological sample is open. Therefore, during this process, problems may arise such as the exposure of pathogens contained in the biological sample to the user or the contamination of the biological sample.

[0009] In addition, the process of injecting multiple biological samples from the sample container into the extraction multi-well plate takes a lot of time, making it difficult to perform rapid detection or multiple detections simultaneously.

[0010] In addition, when installing conductive pipettes using conventional techniques, the presence and uneven tolerances of the ejection end used to manufacture the conductive pipette can lead to problems such as inaccurate installation or leakage of internal samples or solutions. Summary of the Invention

[0011] Technical issues

[0012] The purpose of this invention is to solve the above-mentioned problems by providing a conductive pipette mounting adapter, a sample tube opening and closing device, and an automated sample analysis system. This system opens and closes the sample container within the device to eliminate the user's risk of infection and prevent contamination. It also continuously and automatically performs the opening and closing of the sample sealing components, dispensing, extraction and purification, and amplification detection, thereby significantly reducing the time required for dispensing biological samples.

[0013] Solution to the problem

[0014] To achieve the aforementioned objective, this invention proposes a sample tube opening and closing device, comprising: a housing 110 forming an internal space isolated from the outside, including a door portion 120 for conveying in and out of a porous plate 20 for biological samples having multiple sample tubes 10 for accommodating biological samples; and a sample tube opening and closing portion disposed in the internal space, spaced apart from the porous plate 20 for biological samples, for automatically opening and closing the sample tubes 10.

[0015] The sample tube opening and closing part may include at least one lower clamp 200 that clamps the lower part of the sample tube 10 and at least one upper clamp 300 that clamps the upper part of the sample tube 10.

[0016] In order to simultaneously open and close the three sample tubes 10, the lower clamp 200 and the upper clamp 300 can each be provided in threes and connected in parallel.

[0017] The upper gripper 300 can move linearly in the X, Y and Z directions independently.

[0018] The upper clamp 300 may include: an upper clamping part 310 for clamping the upper part of the sample tube 10; a front-to-back driving part 320 for moving the upper clamping part 310 along the process direction, i.e., the front-to-back direction; a left-to-right driving part 330 for moving the upper clamping part 310 relative to the process direction, i.e., along the left-to-right direction; and an up-and-down driving part 340 for moving the upper clamping part 310 along the up-and-down direction.

[0019] With the lower clamp 200 clamping the lower part of the sample tube 10, the upper clamp 300 clamps the sealing member 12 used to seal the sample tube 10 and rises, thereby opening the sample tube 10.

[0020] With the upper clamp 300 clamping the sealing member 12 for sealing the sample tube 10 and the lower clamp 200 clamping the lower part of the sample tube 10, the sample tube 10 is opened by the relative rotation of the upper clamp 300 and the lower clamp 200.

[0021] The lower clamp 200 may include: a lower clamping part 210 for placing the sample tube 10 and clamping the lower part of the sample tube 10; and a lower clamp rotation drive part 220 for rotating the lower clamping part 210 about a virtual center line passing through the center in a direction perpendicular to the sample tube 10.

[0022] The lower clamp 200 may include an identification code identification unit 230, which is disposed adjacent to the lower clamp 210, and identifies the identification code 13 attached to the sample tube 10, thereby identifying the biological sample.

[0023] The housing 110 may include at least one of the following: a UV lamp portion 130 located on the lower surface of the interior space S and the upper surface of the door portion 120.

[0024] The housing 110 may include an ozone sensor 140 disposed in the internal space S to sense ozone generated by the UV lamp 130.

[0025] The outer casing 110 may include a negative pressure forming part 150, which vents air unidirectionally from the internal space S to the external space to maintain the internal space S in a negative pressure state.

[0026] The negative pressure forming part 150 may include: an exhaust fan 151 located on one side of the housing 110 to exhaust air from the internal space S; and an ozone filter 152 located in front of the exhaust fan 151 to filter ozone from the internal space S.

[0027] Furthermore, this invention discloses an automated sample analysis system, comprising: a sample tube opening and closing device for automatically opening and closing the sealing component 12 of a sample tube 10 containing a biological sample; a dispensing device 1 for dispensing the biological sample from the sample tube 10; an automated purification and extraction device 2 for purifying the biological sample obtained from the dispensing device 1 and extracting target substances; and a nucleic acid amplification and detection device 3 for amplifying and detecting the target substances obtained from the automated purification and extraction device 2.

[0028] The sample tube opening and closing device, the dispensing device 1, and the automatic purification and extraction device 2 form a first internal space S1 isolated from the outside, and the nucleic acid amplification and detection device 3 forms a second internal space S2 isolated from the outside. The first internal space S1 and the second internal space S2 are connected to each other.

[0029] The target substance is the target nucleic acid contained in the biological sample, and quantitative or qualitative detection is performed by amplifying the target nucleic acid.

[0030] The target nucleic acid can be a nucleic acid aptamer or antigen-nucleic acid conjugate that selectively attaches to the antigen or antibody contained in the biological sample.

[0031] It can simultaneously quantitatively or qualitatively detect the nucleic acids, antigens, and antibodies contained in the biological sample.

[0032] The dispensing device 1, the automatic purification and extraction device 2, and the nucleic acid amplification and detection device 3 each have gates that are separate and distinct from each other.

[0033] The dispensing device 1 may include a biological sample dispensing section 400, which moves between the sample tube opening and closing section, the pipette tip holder 80 and the dispensing multi-well plate 30, and draws in the biological sample in the sample tube 10 located in the sample tube opening and closing section and dispenses it into the dispensing multi-well plate 30.

[0034] The biological sample dispensing unit 400 may include: a dispensing pipette 410 for drawing up the biological sample in the sample tube 10 and dispensing the biological sample drawn up by the dispensing porous plate 30; a dispensing pipette sensor unit 420 for adjusting the drawing up and dispensing volume of the biological sample by the dispensing pipette 410; and a dispensing pipette drive unit 430 for driving the dispensing pipette 410 to move between the sample tube 10 located in the first internal space and the dispensing porous plate 30.

[0035] The dispensing pipette sensor unit 420 may include: a pressure sensor 421, which detects the pressure level used to sense whether the dispensing pipette 410 is blocked.

[0036] The dispensing pipette sensor unit 420 may include: a liquid level sensor 422, which is installed on the dispensing pipette 410 to sense whether the dispensing pipette tip containing the biological sample is in contact with the liquid surface of the biological sample.

[0037] The nucleic acid amplification detection device 3 may include: a detection shell forming a detection space isolated from the outside; and a multi-well plate insertion part for inserting a multi-well plate, the multi-well plate having multiple reaction tubes for accommodating the target material extracted by the automatic purification and extraction device 2.

[0038] The multi-well plate insertion portion may include: a support portion for supporting the edge of the multi-well plate having multiple reaction tubes; and a multi-well plate driving portion for driving the support portion between the dispensing position of the automatic purification and extraction device 2 and the amplification position inside the detection housing.

[0039] The detection housing may have a roller shutter door for transporting in and out of the perforated plate insertion section.

[0040] Furthermore, the present invention discloses a conductive pipette mounting adapter for connecting a conductive pipette tip 1000 to a pipette device 1100, comprising: a cylinder body 1200, which is coupled to the pipette device 1100; and a cylindrical leaf spring portion 1300, which is coupled to the outer peripheral surface of the cylinder body 1200, wherein the conductive pipette tip 1000 is inserted and coupled thereto.

[0041] The cylinder body 1200 and the leaf spring portion 1300 may be formed of metal and conductive polymer.

[0042] The cylinder body 1200 may include: an upper connecting portion 1210, which is inserted into and connected to the pipette device 1100; a lower connecting portion 1220, which is exposed to the outside and connected to the conductive pipette tip 1000; and a central portion 1230, which connects the upper connecting portion 1210 and the lower connecting portion 1220, and has a radius smaller than the radii of the upper connecting portion 1210 and the lower connecting portion 1220.

[0043] The upper joint portion 1210 may include: a first upper step portion 1211 formed at the upper end; and a second upper step portion 1212 extended from the lower side of the first upper step portion 1211, with a radius smaller than the radius of the first upper step portion 1211.

[0044] The lower joint portion 1220 may include a plurality of lower step portions 1221 formed at certain intervals between each other.

[0045] The present invention may further include: an elastic member 1400, provided in the lower step portion 1221 to prevent leakage of solution inside the conductive pipette tip 1000.

[0046] The leaf spring portion 1300 may include: an annular portion 1310, which is attached to the upper attachment portion 1210 side; and a plurality of wings 1320 that are elastic along the annular portion 1310 downward side.

[0047] Regarding the leaf spring portion 1300, the annular portion 1310 is disposed on the outer peripheral surface of the second upper step portion 1212, and the wing portion 1320 is disposed separately from the outer peripheral surface of the central portion 1230 and generates elastic deformation through its combination with the conductive pipette tip 1000.

[0048] One end of the leaf spring portion 1300 contacts the PCB portion 1500 disposed within the pipette device 1100 to confirm energization, and the other end is connected to the conductive pipette tip 1000.

[0049] Invention Effects

[0050] The advantages of the conductive pipette mounting adapter, sample tube opening and closing device, and automatic sample analysis system of the present invention are that they can continuously and automatically perform the dispensing, extraction, purification, amplification, and detection of biological samples.

[0051] In particular, the advantages of the conductive pipette mounting adapter, sample tube opening and closing device, and automatic sample analysis system of the present invention are that they can automatically and continuously perform dispensing to detection without user intervention, thereby shortening the detection time and preventing the sample from being contaminated during intermediate movement.

[0052] Furthermore, the advantages of the conductive pipette mounting adapter, sample tube opening and closing device, and automatic sample analysis system of the present invention are that the sample tube containing the biological sample is automatically opened and dispensed while being supplied to the inside in a sealed state, which can prevent contamination of the biological sample.

[0053] Furthermore, the advantages of the conductive pipette mounting adapter, sample tube opening and closing device, and automatic sample analysis system of the present invention are that they can simultaneously detect multiple biological samples or perform different multiple tests on a single biological sample.

[0054] In particular, the advantages of the conductive pipette mounting adapter, sample tube opening and closing device and automatic sample analysis system of the present invention are that, because its components can be used independently in parallel, it can perform three different tests on a biological sample at the same time, or perform tests on multiple biological samples at the same time, which can significantly shorten the detection time.

[0055] Furthermore, the advantages of the conductive pipette mounting adapter, sample tube opening and closing device, and automatic sample analysis system of the present invention are that they overcome the spatial limitations of traditional technologies that can only be used in negative pressure facilities due to the risk of external leakage of harmful substances when detecting viruses. Attached Figure Description

[0056] Figure 1 This is a perspective view that briefly illustrates the appearance of the automatic sample analysis system of the present invention.

[0057] Figure 2 It is shown Figure 1 A plan view of the various trays into which the automated sample analysis system is inserted.

[0058] Figure 3 It is shown Figure 1 A side view of the upper clamp, biological sample dispensing section, and target substance dispensing section in an automated sample analysis system.

[0059] Figure 4 It is shown Figure 1 A three-dimensional view of the upper clamp of the sample tube opening and closing device in an automatic sample analysis system.

[0060] Figure 5 It is shown Figure 4 An enlarged view of a portion of the upper clamp of the sample tube opening and closing device in an automated sample analysis system.

[0061] Figure 6 It is shown Figure 1 A frontal view of the biological sample dispensing section in an automated sample analysis system.

[0062] Figure 7 It is shown Figure 1 A side view of the lower clamp of the sample tube opening and closing device of the automatic sample analysis system.

[0063] Figure 8 It is shown Figure 7 An enlarged view of a portion of the lower clamp of the sample tube opening and closing device in an automated sample analysis system.

[0064] Figure 9 It is shown Figure 1 A three-dimensional view of the lower clamp of the sample tube opening and closing device of the automatic sample analysis system.

[0065] Figure 10 It is shown Figure 1 A diagram showing the state of the negative pressure forming part of the automatic sample analysis system.

[0066] Figure 11 This is a diagram showing the configuration of the conductive pipette mounting adapter of the present invention.

[0067] Figure 12 It is shown Figure 11 A cross-sectional view of the adapter for mounting a conductive pipette.

[0068] Figure 13 It is shown Figure 11 An exploded perspective view of the adapter for installing a conductive pipette.

[0069] Figure 14 It is shown Figure 1 A three-dimensional diagram of the target material delivery unit in an automated sample analysis system.

[0070] Figure 15 It is shown Figure 14 A cross-sectional view of the target material delivery drive section in an automated sample analysis system. Detailed Implementation

[0071] The conductive pipette mounting adapter, sample tube opening and closing device, and automatic sample analysis system of the present invention are described below with reference to the accompanying drawings.

[0072] The automatic sample analysis system of the present invention, as follows: Figures 1 to 3 As shown, it includes: a sample tube opening and closing device that automatically opens and closes the sealing component 12 of the sample tube 10 containing the biological sample; a dispensing device 1 that dispenses the biological sample from the sample tube containing the biological sample in liquid form; an automatic purification and extraction device 2 that purifies the biological sample obtained from the dispensing device 1 and extracts the target substance; and a nucleic acid amplification and detection device 3 that amplifies and detects the target substance obtained from the automatic purification and extraction device 2.

[0073] Regarding the sample tube 10 used, any conventional tube of any shape can be used without restriction, as long as it can contain a liquid sample.

[0074] The automatic purification and extraction device 2 is a component for purifying biological samples obtained from the dispensing device 1 and extracting target substances, and can employ a variety of components.

[0075] For example, the automated purification and extraction device 2 may include: a second outer shell 500 forming a first internal space S1; an extraction section located in the second outer shell 500 for purifying and extracting target substances from biological samples transferred from the dispensing device 1 (described later); and a target substance delivery section 700 that moves between the extraction section and the nucleic acid amplification and detection device 3 and draws in the target substances located in the extraction section for delivery to the nucleic acid amplification and detection device 3.

[0076] The second outer shell 500 is a component that forms the first internal space S1. It can communicate with the outer shell portion 100 of the dispensing device 1 described later. More specifically, it can be connected to the first internal space S1 of the outer shell portion 100 to form the same space.

[0077] In addition, a plurality of holes are formed on the second housing 500 to receive biological samples delivered by the target substance delivery unit 700, and an extraction multi-well plate 40 is provided to which biological samples are dispensed in specific columns and which contains one or more reagents or samples for the purification and extraction of the target substance.

[0078] Furthermore, in addition to the extraction multi-well plate 40, the first internal space S1 on the second outer shell 500 is also provided with an extraction pipette tip holder 50 and a waste section 60. The extraction pipette tip holder 50 is used to supply disposable extraction pipette tips for moving biological samples from the dispensing multi-well plate 30 to the extraction multi-well plate 40 and moving the target material extracted by the nucleic acid amplification detection device 3 from the extraction multi-well plate 40 to the target material delivery section 700.

[0079] In addition, the extraction multi-well plate 40, the extraction pipette tip holder 50, and the waste section 60 are respectively disposed on a second tray that can slide between the first internal space S1 and the external space.

[0080] The second outer casing 500 has a second door 510 that allows the second tray to move between itself and the outside. Through the second door 510, various accessories, such as the extraction perforated plate 40, the extraction pipette tip holder 50, and the waste section 60, can move between the outside and the first internal space S1.

[0081] Additionally, the lower part of the second housing 500 where the extraction porous plate 40 is located may include an extraction section for purifying biological samples dispensed into the extraction porous plate 40 and extracting target substances.

[0082] At this time, the extraction section can be a component of any form disclosed in conventional techniques capable of extracting target substances from biological samples. More specifically, it can include: a magnetic field application section containing a magnet to apply a magnetic field to a specific column of the extraction porous plate 40; and a heating section formed adjacent to the magnetic field application section to heat the same specific column.

[0083] That is, target substances can be purified and extracted from biological samples that have been aliquoted by applying a magnetic field and heating.

[0084] The target material delivery unit 700 is a component that moves between the extraction unit and the nucleic acid amplification and detection device 3 to draw in the target material located in the extraction unit and supply it to the nucleic acid amplification and detection device 3. Various components can be used.

[0085] Furthermore, the target material delivery unit 700 can draw in biological samples from the dispensing porous plate 30 and supply the biological samples to the extraction porous plate 40.

[0086] That is, the target material delivery unit 700 can be equipped with multiple extraction pipette tips of the aforementioned extraction pipette tip holder 50 to draw in the dispensing target material from the dispensing multi-well plate 30 and supply it to the extraction multi-well plate 40, and supply the target material extracted by the extraction multi-well plate 40 to the nucleic acid amplification detection device 3 in the same manner.

[0087] At this time, the target material delivery unit 700 is connected in parallel to form three corresponding to the multi-well plate 20 for biological samples, which can be driven individually to the X-axis, Y-axis and Z-axis respectively.

[0088] Additionally, the target material delivery unit 700 may include: a target material delivery pipette mounting unit 710, which simultaneously mounts multiple extraction pipette tips to draw in and expel biological samples or target materials through the extraction pipette tips; and a target material delivery pipette driving unit, which individually drives the target material delivery pipette mounting unit 710 along the X-axis, Y-axis, and Z-axis respectively.

[0089] More specifically, the target material delivery unit 700, as... Figure 14 and Figure 15 As shown, it may include multiple target material transfer pipette mounting parts 710, a target material transfer plate 720 on which the target material transfer pipette mounting parts 710 are provided, and a target material transfer drive part 730 that drives the target material transfer plate 720 in the left-right direction, i.e., the Y direction.

[0090] Furthermore, the target material delivery unit 700 may include a target material delivery support unit 740 that provides the target material delivery drive unit 730, a target material delivery drive pulley 750 that drives the target material delivery unit 700 in the forward and backward direction, i.e., the X direction, and a drive pulley 760 for driving the target material delivery pipette mounting unit 710.

[0091] The target material transfer plate 720 and the target material transfer pipette mounting part 710 in the target material transfer unit 700, driven by the target material transfer drive unit 730, are described below.

[0092] The target material transfer plate 720 may include: a transfer plate 721, which is provided with a plurality of target material transfer pipette mounting parts 710; and a contact part 722, which is integrally formed on the transfer plate 721 and moves in contact with the target material transfer drive part 730 described later.

[0093] The target material transfer drive unit 730 may include: a cam portion 731, which moves the target material transfer plate 720 in the Y direction (i.e., left-right direction) by pressurizing contact with the contact portion 722; a rotary motor 732, which is connected to the cam portion 731 to rotate the cam portion 731; a rotary bearing 733, which is disposed in a part of the cam portion 731; and a movement guide portion 734, which is disposed on the lower side of the contact portion 722 and guides the left-right movement of the target material transfer plate 720 by moving the guide portion 741 described later.

[0094] The target material transfer support 740 is a component disposed on one side of the target material transfer plate 720 and supporting the target material transfer drive 730. It may include: a guide 741, configured such that the aforementioned movable guide 734 can move to guide the movement of the target material transfer plate 720; and a guide support 742, which is integrally formed with the guide 741.

[0095] The nucleic acid amplification and detection device 3 is a component that amplifies and detects the target material obtained from the automated purification and extraction device 2, and can employ a variety of components.

[0096] For example, the nucleic acid amplification and detection device 3 receives the target material delivered from the target material delivery unit 700, amplifies it, and detects it.

[0097] In addition, the nucleic acid amplification and detection device 3 can receive target material from the target material delivery unit 700 through the detection multi-well plate 70 with multiple wells to perform amplification and detection.

[0098] Furthermore, the nucleic acid amplification detection device 3 may include a third outer shell 800 forming a second internal space S2, wherein the second internal space S2 formed by the third outer shell 800 may be connected to the first internal space S1.

[0099] That is, the first internal space S1, which is used for dispensing biological samples and extracting target substances from biological samples, and the second internal space S2, which performs amplification and detection of the extracted target substances, are interconnected to form a space, enabling efficient automatic analysis of biological samples without user intervention.

[0100] For example, the nucleic acid amplification detection device 3 may include: a detection shell forming a detection space isolated from the outside; and a multi-well plate insertion part for inserting a multi-well plate having multiple reaction tubes for accommodating the target material extracted by the automated purification and extraction device 2.

[0101] At this time, the multi-well plate insertion part may include: a support part that supports the edge of the multi-well plate having a plurality of the reaction tubes; and a multi-well plate driving part that drives the support part between the dispensing position of the automatic purification and extraction device 2 and the amplification position inside the detection shell.

[0102] Furthermore, the detection housing may have a roller shutter door for transporting in and out of the perforated plate insertion portion.

[0103] The dispensing device 1 may include a biological sample dispensing section 400, which moves between the sample tube opening and closing section, the pipette tip holder 80 and the dispensing multi-well plate 30, and draws in the biological sample located in the sample tube 10 in the sample tube opening and closing section and dispenses it into the dispensing multi-well plate 30.

[0104] The biological sample dispensing unit 400 can be a component that moves between the lower clamp 200, the pipette tip holder 80, and the dispensing porous plate 30, and draws in the biological sample located in the sample tube 10 of the lower clamp 200 and dispenses it into the dispensing porous plate 30.

[0105] The biological sample dispensing section 400 corresponds to the lower clamp 200, the upper clamp 300, and the biological sample multi-well plate 20, and is connected in parallel to form at least three, each of which can be driven independently.

[0106] In addition, the biological sample dispensing unit 400 can be driven independently along the X-axis, Y-axis, and Z-axis, and each driving unit is applicable in the same way as the driving units of the aforementioned upper clamp 300.

[0107] In addition, in order to move through the biological sample dispensing unit 400, it is moved from its normal position to the front-back direction by a moving guide 900 located in the front-back direction of the first internal space S1 and the second internal space S2.

[0108] In particular, the biological sample dispensing unit 400 is a component that moves biological samples from the dispensing porous plate 30 located in the first internal space S1 to the extraction porous plate 40, and can move back and forth within the first internal space S1.

[0109] The dispensing pipette 410 can be used to draw biological samples from the sample tube 10 by attaching a disposable pipette tip to the pipette tip holder 80, and dispensing the biological samples drawn in by the dispensing multi-well plate 30.

[0110] In particular, the dispensing pipette 410 is equipped with disposable pipette tips supplied by the pipette tip holder 80. When installed, it is placed in the lower clamping part 210, and draws in biological samples from the sample tube 10 whose sealing part 12 has been opened and moves them to the dispensing multi-well plate 30.

[0111] In addition, the dispensing pipette sensor unit 420 can be a component for adjusting the aspiration and dispensing volume of biological samples from the dispensing pipette 410.

[0112] For example, the dispensing pipette sensor unit 420 may include a pressure sensor 421 for sensing the pressure level to detect whether the dispensing pipette 410 is blocked. More specifically, based on the biological sample dispensing process of the pipette tip attached to the dispensing pipette 410, the pressure level of the dispensing pipette 410 is measured to determine whether the pipette tip is blocked, thereby determining whether dispensing has been completely achieved.

[0113] Furthermore, the pressure of the dispensing pipette 410 is measured by the pressure sensor 421 to ensure that the pressure required to dispense biological samples through the pipette tip is the same, thereby ensuring that the dispensing volume remains consistent.

[0114] The dispensing pipette sensor unit 420 may include a liquid level sensor 422, which senses whether the dispensing pipette tip, which is mounted on the dispensing pipette 410 to contain the biological sample, is in contact with the liquid surface of the biological sample.

[0115] In particular, the biological sample dispensing unit 400 can be equipped with multiple disposable pipette tips from the pipette tip holder 80 to dispense the aspirated biological sample into the dispensing multi-well plate 30. During this process, conductive pipette tips can be used.

[0116] That is, the liquid level sensor 422 performs quantitative dispensing by sensing the liquid level of the biological sample dispensed through the conductive pipette tip.

[0117] Furthermore, the biological sample dispensing unit 400 may include a dispensing pipette driving unit 430, which drives the dispensing pipette 410 to move between the sample tube 10 located in the first internal space S1 and the dispensing porous plate 30.

[0118] At this time, the dispensing pipette drive unit 430 can adopt the same driving method as the aforementioned upper clamp front and rear drive unit 320. In order to drive and guide the biological sample dispensing unit 400 in the process processing direction, it can be driven by the forward and backward movement guide 900.

[0119] The sample tube opening and closing device of the present invention will be described in detail below.

[0120] The sample tube opening and closing device of the present invention is as follows: Figure 1 As shown, it includes: an outer shell 100, including an outer shell 110 forming a first internal space S1 isolated from the outside and a door portion 120 of the outer shell 110 for transporting in and out of a multi-well plate 20 for containing a plurality of sample tubes 10 for biological samples; and a sample tube opening and closing portion, which is separated from the multi-well plate 20 for biological samples and disposed in the internal space, and automatically opens and closes the sample tubes 10.

[0121] At this time, the sample tube opening and closing part may include at least one lower clamp 200 that clamps the lower part of the sample tube 10 and at least one upper clamp 300 that clamps the upper part of the sample tube 10.

[0122] That is, it includes: at least three lower clamps 200, spaced apart from the biosample porous plate 20 and disposed in the internal space, for receiving the sample tube 10 and clamping the lower part of the sample tube 10; and at least three upper clamps 300, clamping the upper part of the sample tube 10, each driven individually, moving between the biosample porous plate 20 and the lower clamps 200 to transfer the sample tube 10.

[0123] The biological sample used for extraction and detection in this invention is a component containing the target substance to be detected, and various components can be used.

[0124] For example, biological samples can include blood, urine, tissue, saliva, sputum, etc., containing target substances obtained from organisms. Furthermore, they can also include pulverized suspensions of animals and plants, culture media of microorganisms, and suspensions.

[0125] At this point, the target substance is the target nucleic acid contained in the biological sample. The automated sample analysis system of the present invention performs quantitative or qualitative detection by amplifying the target nucleic acid.

[0126] In particular, the target substance can be an antigen or antibody contained in a biological sample. In this case, the amplified nucleic acid can be a nucleic acid aptamer or antigen-nucleic acid conjugate contained in the kit that selectively attaches to the antigen or antibody.

[0127] Therefore, the automated sample analysis system of the present invention can simultaneously and quantitatively or qualitatively detect nucleic acids, antigens and antibodies contained in biological samples.

[0128] At this time, the sample tube 10 of the present invention is a component for containing biological samples extracted from the human body, and any disclosed sample tube 10 can be used.

[0129] For example, the sample tube 10 includes: a tube body 11 that contains a biological sample and is open at the top; and a sealing member 12 that applies pressure to the top of the tube body 11 to seal the top of the tube body 11.

[0130] That is, the sample tube 10 may include a sealing member 12 for sealing the biological sample contained in the tube body 11 and engaging with the upper part of the tube body 11. The sealing member 12 is engaged with the tube body 11 by a screw or by a snap-on method, i.e., by simple pressure.

[0131] In addition, the multi-well plate 20 for biological samples is formed by multiple rows and columns of multiple sample tubes 10 to form multiple pores.

[0132] In particular, the biological sample multi-well plate 20 can be connected in parallel to form three 8×4 unit multi-wells with 32 wells each, for a total of 96 wells.

[0133] Additionally, the biosample porous plate 20, as described above as the second tray, is placed on the first tray and moves between the external and internal spaces via the door 120 described later.

[0134] That is, the porous plate 20 for biological samples slides in the state of being placed on the first tray, is transported into the internal space through the door 120, and can be transported out of the internal space.

[0135] The dispensing porous plate 30 is positioned in the processing direction with reference to the biological sample porous plate 20 to the lower clamp 200 described later, and receives the biological sample supplied from the sample tube 10 via the biological sample dispensing section 400.

[0136] At this time, the dispensing multi-well plate 30 may include: multiple holes for receiving biological samples dispensed through the biological sample dispensing section 400; a buffer section for placing other necessary reagents or samples and additional biological samples for sample detection; and a pipette tip holder 80, which is installed in the biological sample dispensing section 400 to supply and discharge disposable pipette tips for moving biological samples.

[0137] The outer shell 100 is a component that forms the internal space, and various components can be used.

[0138] For example, the outer casing 100 may include: an outer casing 110 forming an internal space isolated from the outside; and a door 120 provided in the outer casing 110 for conveying in and out a multi-well plate 20 for biological samples having a plurality of sample tubes 10 for accommodating biological samples.

[0139] Furthermore, the outer casing 100 may include at least one of the following: a UV lamp portion 130, which is located on the lower surface of the interior space or on the upper part of the door portion 120 in the interior space.

[0140] Furthermore, the housing 100 may include an ozone sensor 140, which is disposed in the interior space to sense ozone generated by the UV lamp 130.

[0141] Furthermore, the outer casing 100 may include a negative pressure forming section 150, which vents air unidirectionally from the inner space to the outer space in order to maintain the inner space under negative pressure.

[0142] The door 120 may be a multi-well plate 20 for biological samples, which is provided in the housing 110 for carrying in and out of a plurality of sample tubes 10 for containing biological samples.

[0143] The door 120 can adopt a hinged rotation structure of the open portion of the outer shell 110 to transport the first tray, including the aforementioned porous plate 20 for biological samples, into the internal space.

[0144] The UV lamp part 130 is a component provided on at least one of the lower surface of the interior space or the upper part of the door part 120 in the interior space, and various components may be used.

[0145] For example, the UV lamp 130 can be provided on the lower surface of the interior space and the upper part of the door 120 respectively, to sterilize and disinfect the perforated plate 20 of the first tray that is transported in and out.

[0146] Furthermore, in addition to the internal space, the UV lamp unit 130 may also be provided on at least one of the upper or lower surfaces of the second door portion 510 of the second housing 500.

[0147] The ozone sensor 140 is a component installed in the internal space to sense the ozone generated by the UV lamp unit 130, and various components can be used.

[0148] The ozone sensor 140 can be disposed on the inner wall surface of the housing 110 that constitutes the internal space, and in particular, it can be disposed at a position adjacent to the UV lamp 130 to sense the generated ozone.

[0149] The negative pressure forming part 150 is a component that unidirectionally exhausts air from the internal space to the external space in order to maintain the internal space under negative pressure.

[0150] For example, the negative pressure forming part 150 may include: an exhaust fan 151 located on one side of the outer casing 100 to exhaust air from the interior space; and an ozone filter 152 located in front of the exhaust fan 151 to filter ozone from the interior space.

[0151] More specifically, the negative pressure forming part 150 may have an exhaust port 111 formed on one side of the housing 110, and the exhaust fan 151 is located at the exhaust port 111 to exhaust air unidirectionally from the internal space to the outside.

[0152] The exhaust fan 151 exhausts the air from the internal space, the first internal space S1 and the second internal space S2 connected thereto to the outside, thereby creating a negative pressure in the internal space. Even when the first tray, the second tray, etc. are transported in and out by opening the door 120 and the second door 520, it can prevent the internal pollutants or analytical substances from leaking to the outside.

[0153] The ozone filter 152 may be a component located in front of the exhaust fan 151 to filter ozone in the interior space.

[0154] For example, the ozone filter 152 may be located in front of the interior space of the exhaust fan 151, so that air exhausted from the interior space by the exhaust fan 151 passes through it.

[0155] Accordingly, the ozone filter 152 can prevent ozone generated by the UV lamp unit 130 and the like from being released to the outside and filter ozone.

[0156] In addition, the outer casing 100 of the present invention may also include an exhaust guide 160, which is disposed in the internal space to guide the air exhaust path from the internal space to the outside.

[0157] The exhaust guide 160 is vertically disposed on the opposite side of the door 120 of the first tray and the second tray in the interior space, guiding the air in the first interior space S1 and the second interior space S2 toward the exhaust port 111.

[0158] Furthermore, the outer casing 100 may include an external air intake filter 190, which is disposed on the upper surface to form an airflow in the internal space.

[0159] At this time, the external air intake filter 190 is disposed on the upper surface of the housing 110 to guide external air to be supplied to the internal space, and at the same time has a filter for filtering external air.

[0160] Accordingly, the external air intake filter 190, together with the internal circulation fan 180 described later, guides the formation of airflow in the internal space, so that the airflow in the internal space is smoothly discharged through the exhaust fan 151.

[0161] Additionally, the outer casing 100 may include an internal circulation fan 180, located at one end of the internal space, to guide the formation of airflow within the internal space.

[0162] The internal circulation fan 180 is driven at one end of the internal space to form an airflow inside, so that the filtered external air supplied from the outside through the external air intake filter 190 circulates in the internal space and is guided to exhaust in the direction of the aforementioned exhaust fan 151.

[0163] The lower clamp 200 is a component that is separated from the porous plate 20 for biological samples and disposed in the internal space, receiving the sample tube 10 and clamping the lower part of the sample tube 10.

[0164] In particular, three lower clamps 200 are arranged in parallel corresponding to the aforementioned multi-well plate 20 for biological samples, thereby clamping the sample tubes 10 respectively to perform three independent tests.

[0165] For example, the lower clamp 200 may include: a lower clamping part 210 for placing the sample tube 10 and clamping the lower part of the sample tube 10; and a lower clamp rotation drive part 220 for rotating the lower clamping part 210 about a virtual center line passing through the center in a direction perpendicular to the sample tube 10.

[0166] Furthermore, the lower clamp 200 may include an identification code identification unit 230, which is disposed adjacent to the lower clamp 210, and identifies the biological sample by identifying the identification code 13.

[0167] The lower clamping part 210 may be a component that is adjacent to the porous plate 20 for biological samples disposed in the internal space, and holds the sample tube 10 that moves through the upper clamping device 300 and clamps the lower part of the sample tube 10.

[0168] For example, the lower clamping part 210 may include: a lower clamping component 211, which is provided in a plurality of manner on the outer periphery of the sample tube 10 in the circumferential direction, with the placement position of the sample tube 10 as a reference; and a lower clamping component driving part 212, which drives the lower clamping component 211 so that the lower clamping component 211 moves in the radial direction with the placement position of the sample tube 10 as a reference.

[0169] Furthermore, the lower clamping part 210 may include a rubber fixing part 213, which is disposed on the lower clamping part 211 and is close to the clamped sample tube 10 to fix the sample tube 10.

[0170] That is, when the sample tube 10 is placed in the placement position, the lower clamping part 210 moves the lower clamping component 211 horizontally toward the sample tube 10, thereby clamping the lower part of the sample tube 10.

[0171] At this time, the lower clamping member drive unit 212 can adopt any form of drive disclosed previously, such as by using an electric motor, a magnetic motor and gears, by using pulleys and conveyor belts, or by using electromagnetic movement, so that the lower clamping member 211 moves linearly.

[0172] The lower clamp rotation drive unit 220 can be a component that rotates around a virtual center line passing through the center in a vertical direction to the sample tube 10.

[0173] That is, the lower clamp rotation drive 220 causes the lower clamp 210 to rotate about a virtual center line passing through the center in the vertical direction of the sample tube 10, thereby causing the sample tube 10 to rotate in place.

[0174] Accordingly, the lower clamp rotation drive 220 causes the tube body 11 of the sample tube 10 to rotate relative to the sealing member 12 held by the upper clamp 300, thereby separating the sealing member 12 of the sample tube 10 from the tube body 11.

[0175] Accordingly, the tube body 11 can be opened automatically from the inside, which will be described in detail below.

[0176] Additionally, the lower gripper rotation drive unit 220 may include: a lower gripper rotation drive source 221 that provides rotation-based driving force; and a pair of lower gripper rotation pulleys 222, one connected to the lower gripper rotation drive source 221 and the other connected to the lower gripper unit 210 and connected to each other via conveyor belts.

[0177] That is, the lower gripper rotation drive unit 220 operates as follows: when the lower gripper rotation drive source 221 provides rotation to one lower gripper rotation pulley 222, the other lower gripper rotation pulley 222 connected by the conveyor belt will rotate, and the lower gripper unit 210 connected to it will also rotate.

[0178] Additionally, the lower clamp 200 may also include an identification code identification unit 230, which identifies the identification code 13 attached to the sample tube 10, thereby identifying the biological sample.

[0179] For example, the identification code identification unit 230 can be disposed adjacent to the lower clamping unit 210 to identify biological samples by identifying the identification code 13.

[0180] At this time, the identification code identification unit 230 may include: an identification unit 232, which is disposed adjacent to the lower clamping unit, illuminates the identification code 13 with light, and receives the illuminated light to identify the identification code 13; and a reading unit 231, which is disposed adjacent to the identification unit 232, and reads the identification code 13 identified by the identification unit 232.

[0181] In particular, the identification code recognition unit 230 can rotate around the lower clamping part 210 and the placed sample tube 10 as a central axis, thereby enabling stable identification regardless of the position of the identification code 13 attached to the tube body 11.

[0182] Alternatively, the identification code recognition unit 230 can be fixedly set. In this case, when the upper clamp 300 moves the sample tube 10 from the biological sample multi-well plate 20, the position of the sample tube 10 is kept in a fixed position by sorting, thereby enabling stable identification of the identification code.

[0183] The identification code 13 can be any component that can be scanned and identified, such as a barcode, QR code, etc.

[0184] The upper clamp 300 can be a component that is driven independently, thereby clamping the upper part of the sample tube 10 and moving it between the multi-well plate 20 for biological samples and the lower clamp 200 to transfer the sample tube 10.

[0185] In particular, the upper clamp 300 corresponds to the aforementioned porous plate 20 for biological samples and the lower clamp 200, and three are arranged in parallel and driven individually.

[0186] In particular, the upper gripper 300 can move linearly in the X, Y and Z directions, and the three upper grippers 300 are driven independently.

[0187] For example, the upper clamp 300 may include: an upper clamping part 310 for clamping the upper part of the sample tube 10; a front-to-back driving part 320 for moving the upper clamping part 310 along the process direction, i.e., the front-to-back direction; a left-to-right driving part 330 for moving the upper clamping part 310 relative to the process direction, i.e., along the left-to-right direction; and an up-and-down driving part 340 for moving the upper clamping part 310 along the up-and-down direction.

[0188] The upper clamping part 310 may be a component that clamps the upper part of the sample tube 10.

[0189] For example, the upper clamping part 310 may include: a plurality of upper clamping components 311, which clamp the tube body 11 or sealing component 12 of the sample tube 10, and are positioned on the outer peripheral surface of the sample tube 10 with the sample tube 10 as the center, in the same manner as the aforementioned lower clamping component 211; and an upper clamping component driving part 312, which drives the upper clamping components 311 in the radial direction of the sample tube 10.

[0190] At this time, the upper clamping component 311 forms a friction part 313 on the contact surface of the sample tube 10 to stably clamp the sample tube 10.

[0191] More specifically, when the plurality of upper clamping portions 310 are positioned adjacent to the outer peripheral surface of the corresponding sample tube 1, they move in the radial direction to clamp the sample tube 10.

[0192] The upper clamping member drive unit 312 can, like the aforementioned lower clamping member drive unit 212, move the upper clamping member 311 horizontally to clamp the sample tube 10 or release the sample tube 10 by various methods disclosed previously.

[0193] The upper clamping unit 320 can be a component that moves the upper clamping unit 310 along the process processing direction, i.e., the front-back direction.

[0194] The upper clamping unit 320 can drive the upper clamping unit 310 in the forward and backward direction through a pair of upper clamping unit front and rear drive pulleys connected to the upper clamping unit 310 by a conveyor belt.

[0195] Furthermore, the upper clamping unit 320 is driven in the front-back direction at a fixed position by a front-back movement guide 900 provided in the first internal space S1 and the second internal space S2, which causes the upper clamping unit 310 to move through.

[0196] The upper clamping unit 330 can be a component that allows the upper clamping unit 310 to move in the left-right direction relative to the process processing direction.

[0197] At this time, the upper clamping device left and right drive part 330, as described above, can be a pair of pulleys and a conveyor belt connected to the upper clamping part 310. As another example, it can be driven left and right by rotating the screw.

[0198] The upper clamping unit 340 is a component that moves the upper clamping unit 310 in the vertical direction. It can utilize the aforementioned pulleys and conveyor belt, or, as another example, it can be a component driven by rotating screws.

[0199] Additionally, the following describes how the sample tube 10 is opened using the aforementioned upper clamp 300 and lower clamp 200.

[0200] As previously described, the sample tube 10 can be connected to the tube body 11 by a screw-type sealing component 12, or by a snap-on type, i.e., by simple pressure application.

[0201] In addition, if the sealing component 12 is screwed to the tube body 11, the lower part of the tube body 11 is clamped by the lower clamping part 210 while the sealing component 12 is clamped by the upper clamping part 310.

[0202] At this time, if the lower clamping part 210, including the sample tube 10, is rotated as a whole by the lower clamping drive part 220, the sealing member 12 can rotate relative to the tube body 11 because the sealing member 12 is clamped by the upper clamping part 310. Accordingly, the sealing member 12 can be separated from the tube body 11.

[0203] Alternatively, the sealing member 12 can be opened by rotating the upper clamping part 310 while the clamping tube body 11 is fixed by the lower clamping part 210.

[0204] In addition, if the sealing member 12 is attached to the tube body 11 by a simple pressure application, then as described above, with the sealing member 12 held by the upper clamping part 310 and the lower part of the tube body 11 held by the lower clamping part 210, the sealing member 12 can be opened by moving the upper clamping part 310 upward or the lower clamping part 210 downward.

[0205] The conductive pipette mounting adapter of the present invention is described below.

[0206] In addition, the conductive pipette mounting adapter described below is applicable to various components for mounting conductive pipettes in automated sample analysis systems. More specifically, it can be set in the biological sample dispensing section 400 to more stably and easily achieve the mounting of conductive pipette tips.

[0207] The conductive pipette mounting adapter of the present invention, such as Figures 11 to 13As shown, a conductive pipette tip 1000 is connected to a pipette assembly 1100, comprising: a cylinder body 1200, which is attached to the pipette assembly 1100; and a cylindrical leaf spring portion 1300, which is attached to the outer peripheral surface of the cylinder body 1200, wherein the conductive pipette tip 1000 is inserted and attached.

[0208] The pipette device 1100 may be a component that is combined with the conductive pipette tip 1000 to move or dispense the solution contained in the conductive pipette tip 1000.

[0209] At this time, the pipette device 1100 may have a conductive element for confirming whether the conductive pipette tip 1000 is engaged or not. For example, it may have a PCB section 1500 described later that can be energized.

[0210] The cylinder body 1200 is a component that is attached to the pipette device 1100, and various components can be used.

[0211] For example, the cylinder body 1200 may include: an upper connecting portion 1210, which is inserted into and connected to the pipette device 1100; a lower connecting portion 1220, which is exposed to the outside and connected to the conductive pipette tip 1000; and a central portion 1230, which connects the upper connecting portion 1210 and the lower connecting portion 1220, and has a radius smaller than the radii of the upper connecting portion 1210 and the lower connecting portion 1220.

[0212] At this time, the upper joint portion 1210 may include: a first upper step portion 1211, formed at the upper end; and a second upper step portion 1212, extended from the lower side of the first upper step portion 1211, with a radius smaller than the radius of the first upper step portion 1211.

[0213] Furthermore, the lower joint portion 1220 may include a plurality of lower step portions 1221 formed at certain intervals in the upward and downward directions.

[0214] The leaf spring portion 1300 may be a cylindrical component that is attached to the outer peripheral surface of the cylinder body 1200 and into which the conductive pipette tip 1000 is inserted.

[0215] For example, the leaf spring portion 1300 may include: an annular portion 1310, which is attached to the upper attachment portion 1210 side; and a plurality of wings 1320 that are elastic along the annular portion 1310 downward side.

[0216] Furthermore, regarding the leaf spring portion 1300, the wing portion 1320 is provided spaced apart from the outer peripheral surface of the central portion 1230, thereby generating elastic deformation through its combination with the conductive pipette tip 1000, and the annular portion 1310 is provided on the outer peripheral surface of the second upper step portion 1212.

[0217] Accordingly, the leaf spring portion 1300 can be guided to allow the conductive pipette tip 1000 to elastically adhere to the position of the wing portion 1320 and be stably engaged.

[0218] Furthermore, one end of the leaf spring portion 1300 contacts the PCB portion 1500 disposed within the pipette device 1100 to confirm energization, and the other end is connected to the conductive pipette tip 1000.

[0219] In addition, the cylinder body 1200 and the leaf spring portion 1300 may be formed of metal and conductive polymer.

[0220] Furthermore, the conductive pipette mounting adapter of the present invention may also include: an elastic member 1400, which is provided in the lower step portion 1221 to prevent leakage of solution inside the conductive pipette tip 1000.

[0221] At this time, the elastic member 1400 is provided on the outer diameter of the lower step portion 1221 and fits tightly against the inner diameter of the conductive pipette tip 1000, thereby preventing the solution contained in the conductive pipette tip 1000 from leaking.

[0222] Furthermore, the elastic member 1400 can also be provided in the first upper step portion 1211.

[0223] Furthermore, the adapter for mounting the conductive pipette may also include: an adapter plate 1600, which is bolted to the lower end of the pipette device 1100 for easy removal of the conductive pipette tip 1000 from the pipette device 1100.

[0224] The adapter plate 1600 is bolted to the lower end of the pipette device 1100. When the adapter plate 1600 is removed while the upper end of the conductive pipette tip 1000 is disturbed, it is guided to remove the conductive pipette tip 1000 together.

[0225] The above description is only a part of the preferred embodiments that the present invention can present. As is well known, the scope of the present invention should not be limited to the above embodiments. It should be understood that the technical ideas and fundamental technical ideas of the present invention described above are all included within the scope of the present invention.

Claims

1. A sample tube opening and closing device, characterized in that, include: The outer shell (110) forms an internal space (S) isolated from the outside, including a door (120) for conveying in and out of a multi-well plate (20) for biological samples having multiple sample tubes (10) for containing biological samples; The sample tube opening and closing part is separated from the biological sample multi-well plate (20) and disposed in the internal space (S), automatically opening and closing the sample tube (10). The sample tube opening and closing part includes at least one lower clamp (200) that clamps the lower part of the sample tube (10) and at least one upper clamp (300) that clamps the upper part of the sample tube (10). The upper clamp (300) includes: Multiple upper clamping components (311) are disposed at positions corresponding to the outer peripheral surface of the sample tube (10) to move and open / close the sample tube (10); and The upper clamping component drive unit (312) horizontally moves the upper clamping component (311) to clamp and release the sample tube (10). The upper clamp (300) clamps the sealing member (12) for sealing the sample tube (10) and moves the sample tube (10) to the lower clamp (200). While maintaining the clamping of the sealing member (12), the lower clamp (200) clamps the lower part of the sample tube (10). The sample tube (10) is opened by raising the upper clamp (300) relative to the lower clamp (200) or rotating the lower clamp (200) relative to the upper clamp (300).

2. The sample tube opening and closing device according to claim 1, characterized in that, In order to simultaneously open and close the three sample tubes (10), three lower clamps (200) and three upper clamps (300) are respectively provided and connected in parallel.

3. The sample tube opening and closing device according to claim 2, characterized in that, The upper gripper (300) moves independently in the X, Y and Z directions in a linear fashion.

4. The sample tube opening and closing device according to claim 3, characterized in that, The upper clamp (300) includes: The upper clamping part (310) clamps the upper part of the sample tube (10); The upper clamping unit (320) drives the upper clamping unit (310) to move along the process processing direction, i.e., the front-back direction; The upper clamping unit (330) drives the upper clamping unit (310) to move relative to the process processing direction, i.e., along the left and right direction. The upper clamping unit (340) drives the upper clamping unit (310) to move in the vertical direction.

5. The sample tube opening and closing device according to claim 1, characterized in that, The upper clamp (300) rises while the lower clamp (200) is holding the lower part of the sample tube (10), clamping the sealing component (12) for sealing the sample tube (10), thereby opening the sample tube (10).

6. The sample tube opening and closing device according to claim 1, characterized in that, With the upper clamp (300) clamping the sealing member (12) for sealing the sample tube (10) and the lower clamp (200) clamping the lower part of the sample tube (10), the sample tube (10) is opened by the relative rotation of the upper clamp (300) and the lower clamp (200).

7. The sample tube opening and closing device according to claim 1, characterized in that, The lower clamp (200) includes: The lower clamping part (210) is used to place the sample tube (10) and clamp the lower part of the sample tube (10); The lower clamp rotation drive (220) causes the lower clamp (210) to rotate about a virtual center line passing through the center in a direction perpendicular to the sample tube (10).

8. The sample tube opening and closing device according to claim 7, characterized in that, The lower clamp (200) includes an identification code identification unit (230) disposed adjacent to the lower clamp (210) to identify the identification code (13) attached to the sample tube (10), thereby identifying the biological sample.

9. The sample tube opening and closing device according to claim 1, characterized in that, The housing (110) includes at least one of a UV lamp portion (130) located on the lower surface of the interior space (S) and the upper surface of the door portion (120).

10. The sample tube opening and closing device according to claim 9, characterized in that, The housing (110) includes an ozone sensor (140) disposed in the interior space (S) to sense ozone generated by the UV lamp (130).

11. The sample tube opening and closing device according to claim 1, characterized in that, The outer casing (110) includes a negative pressure forming part (150) that vents air unidirectionally from the inner space (S) to the outer space to maintain the inner space (S) in a negative pressure state.

12. The sample tube opening and closing device according to claim 11, characterized in that, The negative pressure forming part (150) includes: an exhaust fan (151) located on one side of the housing (110) to exhaust air from the internal space (S); An ozone filter (152) is located in front of the exhaust fan (151) to filter ozone from the interior space (S).

13. An automated sample analysis system, characterized in that, include: The sample tube opening and closing device according to any one of claims 1 to 12 automatically opens and closes the sealing component (12) of the sample tube (10) containing biological samples; Dispensing device (1) dispenses the biological sample from the sample tube (10); An automated purification and extraction device (2) is used to purify and extract target substances from the biological sample obtained from the dispensing device (1); Nucleic acid amplification and detection device (3) amplifies and detects the target substance obtained from the automated purification and extraction device (2).

14. The automated sample analysis system according to claim 13, characterized in that, The sample tube opening and closing device, the dispensing device (1) and the automatic purification and extraction device (2) form a first internal space (S1) isolated from the outside, and the nucleic acid amplification and detection device (3) forms a second internal space (S2) isolated from the outside. The first internal space (S1) and the second internal space (S2) are connected to each other.

15. The automated sample analysis system according to claim 13, characterized in that, The target substance is the target nucleic acid contained in the biological sample, and quantitative or qualitative detection is performed by amplifying the target nucleic acid.

16. The automated sample analysis system according to claim 15, characterized in that, The target nucleic acid is a nucleic acid aptamer or antigen-nucleic acid conjugate that selectively attaches to the antigen or antibody contained in the biological sample.

17. The automated sample analysis system according to claim 13, characterized in that, Simultaneously, quantitative or qualitative detection of nucleic acids, antigens, and antibodies contained in the biological sample is performed.

18. The automated sample analysis system according to claim 13, characterized in that, The dispensing device (1), the automatic purification and extraction device (2), and the nucleic acid amplification and detection device (3) each have gates that are separate from each other.

19. The automated sample analysis system according to claim 14, characterized in that, The dispensing device (1) includes a biological sample dispensing section (400) that moves between the sample tube opening and closing section, the pipette tip holder (80), and the dispensing multi-well plate (30) to draw in biological samples located in the sample tube (10) at the sample tube opening and closing section and dispense them into the dispensing multi-well plate (30).

20. The automatic sample analysis system according to claim 19, characterized in that, The biological sample dispensing unit (400) includes: A dispensing pipette (410) is used to aspirate the biological sample from the sample tube (10) and dispense the biological sample aspirated by the dispensing multi-well plate (30). The dispensing pipette sensor section (420) is used to adjust the aspiration and dispensing volume of the biological sample in the dispensing pipette (410); The dispensing pipette drive unit (430) drives the dispensing pipette (410) to move between the sample tube (10) located in the first internal space (S1) and the dispensing porous plate (30).

21. The automatic sample analysis system according to claim 20, characterized in that, The dispensing pipette sensor unit (420) includes a pressure sensor (421) for routine detection to sense the pressure level of whether the dispensing pipette (410) is blocked.

22. The automatic sample analysis system according to claim 20, characterized in that, The dispensing pipette sensor unit (420) includes a liquid level sensor (422) which is installed on the dispensing pipette (410) to sense whether the dispensing pipette tip containing the biological sample is in contact with the liquid surface of the biological sample.

23. The automatic sample analysis system according to claim 13, characterized in that, The nucleic acid amplification and detection device (3) includes: The outer casing is inspected to create an inspection space isolated from the outside. A multi-well plate insertion section is used to insert a multi-well plate, which has multiple reaction tubes for accommodating the target material extracted by the automated purification and extraction device (2).

24. The automatic sample analysis system according to claim 23, characterized in that, The multi-well plate insertion portion includes: a support portion for supporting the edge of the multi-well plate having multiple reaction tubes; and a multi-well plate driving portion for driving the support portion between the dispensing position of the automatic purification and extraction device (2) and the amplification position inside the detection housing.

25. The automatic sample analysis system according to claim 23, characterized in that, The detection housing has a roller shutter door for transporting in and out of the perforated plate insertion section.

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