A cord blood stem cell cryobank

Abstract

The application discloses a kind of umbilical cord blood stem cell frozen storage, belong to stem cell preservation technical field, including stem cell preservation bag;It further includes library body and storage box;Library body is provided with moving frame in, moving frame is provided with push box in;Moving frame both sides are distributed with liquid nitrogen preservation box;Liquid nitrogen preservation box back is connected liquid nitrogen storage tank by nitrogen supplementing pipe;Library body front is provided with placing cavity;Liquid nitrogen preservation box upper end is provided with the box cover that can be lifted and opened, and the tray is installed in box cover bottom;Push box front and both sides are open;The inner chamber top of push box is provided with the push rod that can be lifted and lowered and transverse movement;Push rod both ends are provided with the clamping rod for clamping storage box;Push box back is provided with push plate.The application it realizes the full-automatic quick storage and take out of umbilical cord blood stem cell, uses single small liquid nitrogen preservation box to preserve to each umbilical cord blood stem cell frozen bag, improves the preservation quality, realizes the quick identification and accurate storage management of umbilical cord blood stem cell.

Description

Technical Field

[0001] This invention belongs to the field of stem cell preservation technology, specifically a cryopreservation bank for umbilical cord blood stem cells. Background Technology

[0002] A cord blood bank is short for a cord blood hematopoietic stem cell bank. It is used to store the cord blood of newborns, mainly to preserve the rich hematopoietic stem cells in it. It can reserve resources for patients who need hematopoietic stem cell transplantation and provide stem cell matching information. Internationally, it is also known as a cord blood bank or life bank.

[0003] Umbilical cord blood hematopoietic stem cells can withstand cryopreservation and be stored long-term, ready for use at any time. The process from finding unrelated umbilical cord blood to obtaining it takes only 2-3 weeks, which is 2-5 months shorter than the entire process for finding and collecting unrelated peripheral blood or bone marrow hematopoietic stem cell transplant donors. Furthermore, umbilical cord blood banks are physical cryopreservation stem cell banks, allowing for immediate access to the blood and avoiding many uncertainties, such as the risk of donors terminating donations during the collection and donation process, associated with bone marrow and peripheral blood hematopoietic stem cell transplants.

[0004] After collection, umbilical cord blood stem cells are stored in special cryopreservation bags. These bags are highly durable and have excellent sealing properties, allowing them to withstand low-temperature freezing. The cryopreservation bags are typically first placed in a temporary liquid nitrogen tank for storage, and then transferred together to a larger liquid nitrogen tank within the bank. To prevent damage to the hematopoietic stem cells during the cooling process and in the low-temperature environment, a cryoprotectant is added to the umbilical cord blood hematopoietic stem cells before cooling.

[0005] Currently, the storage of umbilical cord blood stem cells in storage banks is mainly done manually by opening liquid nitrogen tanks. In order to identify the type of umbilical cord blood stem cells, the labels on the frozen bags are usually identified manually. Therefore, when retrieving umbilical cord blood stem cells, it takes a long time for people to manually search for the corresponding number of umbilical cord blood stem cells in the storage tank. As a result, the efficiency of umbilical cord blood stem cell storage and retrieval is low, and rapid identification and automatic management and storage of umbilical cord blood stem cells have not been achieved. Summary of the Invention

[0006] To address the above problems, this invention provides a cryopreservation bank for umbilical cord blood stem cells, which enables fully automated and rapid storage and retrieval of umbilical cord blood stem cells. Each umbilical cord blood stem cell cryopreservation bag is stored in a single small liquid nitrogen storage box, which improves the preservation quality and enables rapid identification and accurate storage management of umbilical cord blood stem cells.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] A cryopreservation bank for umbilical cord blood stem cells includes stem cell preservation bags; it also includes a bank body and storage boxes for placing the stem cell preservation bags; the bank body is provided with at least one longitudinally movable frame, and the movable frame is provided with a vertically movable push box; liquid nitrogen preservation tanks are distributed on both sides of the movable frame in the bank body; the back of the liquid nitrogen preservation tank is connected to a liquid nitrogen storage tank through a nitrogen replenishment pipe; the front of the bank body is provided with a placement cavity for placing the storage boxes; the upper end of the liquid nitrogen preservation tank is provided with a liftable and openable lid, and the bottom of the lid is provided with a tray for placing the storage boxes; the push box is open on the front and sides; the top surface of the inner cavity of the push box is provided with a push rod that can be lifted vertically and moved laterally; the two ends of the push rod are provided with clamping rods for clamping the storage boxes; the back of the push box is provided with a push plate.

[0009] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0010] The stem cell preservation bag is a soft bag, but placing it inside a storage box to form a hard box provides better protection and also facilitates more automated management. After the storage box is inserted into the placement cavity, the pusher box automatically removes the storage box from the placement cavity and, in conjunction with the moving frame, automatically moves it to the corresponding liquid nitrogen storage box, storing the storage box there. When it is necessary to remove the corresponding stem cell preservation bag, the pusher box is used to automatically remove it and transport it to the placement cavity for retrieval.

[0011] The inner height of the push box is greater than the height of the storage box, allowing the push rod to move left and right with sufficient space when it is at the top position without affecting the position of the storage box. The clamping rods at both ends of the push rod can hold the storage box and then move it laterally to send the storage box into the corresponding liquid nitrogen storage box, or to take the storage box out of the liquid nitrogen storage box. The clamping rods can rotate 180°, so it is possible to store or retrieve materials from the liquid nitrogen storage boxes on the left and right sides of the push box. The push plate on the back of the push box can push the storage box into the placement cavity.

[0012] As a further improvement to the above solution, a vertical shaft is provided at the upper end of the push rod; the vertical shaft passes through the slide block; the slide block is movably mounted on the lead screw; the lead screw is mounted on the top of the push box; the lead screw is driven to rotate by a drive motor; a third lifter is provided on the slide block; the third lifter drives the vertical shaft to move up and down.

[0013] The technical objective of the above improvement is to drive the vertical shaft to move up and down via the third lifter, thereby controlling the up and down movement of the push rod. Since the height of the inner cavity of the push box is higher than the height of the storage box, when the push rod is raised to the top, it will not affect the placement of the storage box. This makes it easier for the push rod to move left and right at the top to the left or right side of the storage box, thus achieving position adjustment of the push rod.

[0014] As a further improvement to the above solution, two lead screws are arranged parallel to each other; the two ends of the slide are mounted on the lead screws; a driven gear is provided at the end of the lead screw; the driven gears mesh with each other through a transmission gear; one of the driven gears meshes with a drive gear on the motor shaft of the drive motor.

[0015] The technical objective of the above improvement is to drive the gear to rotate via a drive motor, which in turn drives the driven gear to rotate. The driven gear then drives another driven gear to rotate via a transmission gear, thereby driving the two lead screws to rotate simultaneously. This causes the slide to move on the lead screws, and the slide in turn drives the push rod to move.

[0016] As a further improvement to the above solution, a rotatable spline sleeve is provided inside the push rod, and a spline shaft is sleeved inside the spline sleeve. The front end of the spline shaft is connected to a clamping rod, and the rear end is rotatably connected to a first telescopic rod. The outer wall of the spline sleeve is provided with threads and a threaded sleeve is sleeved on it. The threaded sleeve is connected to a second telescopic rod. The second telescopic rod drives the threaded sleeve to move axially, thereby driving the spline sleeve to rotate. The spline sleeve drives the spline shaft to rotate, thereby driving the clamping rod to rotate. The extension and retraction of the first telescopic rod drives the extension and retraction of the spline shaft, thereby driving the clamping rod to open and close.

[0017] The above-mentioned technical objectives are: to realize the rotation angle of the clamping rod, and to realize the clamping and loosening of the clamping rod; when the telescopic rod drives the threaded sleeve to move axially, it drives the spline sleeve to rotate, the spline sleeve drives the spline shaft to rotate, thereby driving the clamping rod to rotate to adjust the angle; the first telescopic rod drives the spline shaft to extend and retract, thereby driving the clamping rod to open and close.

[0018] As a further improvement to the above solution, the pusher plate is connected to a third telescopic rod; the third telescopic rod is installed at the bottom of the pusher box.

[0019] The technical objective of the above improvement is to drive the pusher plate to move via the third telescopic rod, thereby pushing the storage box inside the pusher box into the placement cavity.

[0020] As a further improvement to the above solution, positioning grooves are distributed on the side wall of the storage box; positioning protrusions for engaging with the positioning grooves are provided on the clamping surface of the clamping rod.

[0021] The technical objective of the above improvements is to enhance the stability of the clamping rod in holding the storage box by cooperating with the positioning groove and the positioning protrusion.

[0022] As a further improvement to the above solution, lifting wheels are provided on the upper and lower side walls of the push box; wherein the lower lifting wheel is connected to a gear transmission mechanism through a belt transmission mechanism; and the gear transmission mechanism is connected to a lifting motor.

[0023] The technical objective of the above improvements is to drive the lifting wheels to rotate via a lifting motor, thereby driving the push box to move up and down. The lifting wheels can be gears, and the moving frame has a rack that meshes with the gears, thereby improving the stability of the push box's up and down movement.

[0024] As a further improvement to the above solution, the upper and lower ends of the movable frame are connected to a lead screw mechanism; the tail ends of the lead screw mechanism are connected to each other through a belt drive mechanism.

[0025] The technical objective of the above improvements is to drive the moving frame to move back and forth through a lead screw mechanism, and the two lead screw mechanisms can improve its movement stability.

[0026] As a further improvement to the above solution, a conveyor belt mechanism is provided on the top and bottom surfaces of the placement cavity; a lifting door is provided at the outlet of the placement cavity; the lifting door is connected to the first lifter.

[0027] The above-mentioned technical objectives are: the conveyor belt mechanism facilitates the automatic transfer of storage boxes from the placement cavity to the push box, or the reverse rotation transfers storage boxes from the push box to the placement cavity; the lifting door serves to isolate the outlet of the placement cavity.

[0028] As a further improvement to the above solution, the front of the storage unit is equipped with a display screen and operation buttons.

[0029] The technical objective of the above improvements is to allow users to observe the status of each liquid nitrogen storage box or the number of the storage box on the display screen, so as to facilitate the removal or storage of the storage box number. The operation buttons include a storage button and a retrieval button. Attached Figure Description

[0030] Figure 1 This is a side cross-sectional view of the present invention.

[0031] Figure 2 This is a schematic diagram of the side structure of the cavity.

[0032] Figure 3 This is a front structural diagram of the present invention.

[0033] Figure 4 This is a top view of the internal structure of the present invention.

[0034] Figure 5 This is a side view of the liquid nitrogen storage box.

[0035] Figure 6 This is a schematic diagram of the mobile frame.

[0036] Figure 7 A diagram illustrating the storage of stem cell preservation bags within a storage box.

[0037] Figure 8 This is a front cross-sectional view of the push box.

[0038] Figure 9 This is a side sectional view of the push box.

[0039] Figure 10 This is a schematic diagram of the push rod structure.

[0040] Figure 11 This is a schematic diagram of the drive mechanism of the push rod.

[0041] Figure 12 This is a schematic diagram of the lifting wheel drive mechanism at the bottom of the push box.

[0042] Figure 13 This is a schematic diagram of the structure in which the clamping rod rotates to the left under the drive of the drive mechanism.

[0043] Figure 14 This is a schematic diagram of the structure in which the clamping rod rotates to the right under the drive of the drive mechanism.

[0044] In the diagram: 1. Belt drive mechanism; 2. Liquid nitrogen storage tank; 3. Storage chamber; 4. Lead screw mechanism; 5. Moving frame; 6. Control box; 7. First lifter; 8. Push box; 9. Lifting door; 10. Conveyor belt mechanism; 11. Placement cavity; 12. Liquid nitrogen storage tank; 13. Stem cell storage bag; 14. Storage box; 15. Positioning groove; 16. Lead screw mechanism; 17. Lifting wheel; 18. Second lifter; 19. Slide; 20. Push rod; 21. First telescopic rod; 22. Second telescopic rod; 23. Rotary joint; 24. 25. Splined shaft; 26. Threaded sleeve; 27. Splined sleeve; 28. Clamping rod; 29. ​​Positioning protrusion; 30. Vertical shaft; 31. Third lifting device; 32. Lead screw; 33. Transmission gear; 34. Drive gear; 35. Drive motor; 36. Display screen; 37. Control button; 38. Push plate; 39. Third telescopic rod; 171. Belt drive mechanism; 172. Lifting motor; 173. Gear drive mechanism; 201. Fourth lifting device; 202. Box cover; 203. Tray; 204. Nitrogen replenishment pipe. Detailed Implementation

[0045] To enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be described in detail below with reference to embodiments. The description in this part is only exemplary and explanatory, and should not be used to limit the scope of protection of the present invention in any way.

[0046] Please see Figures 1 to 14In one specific embodiment, an umbilical cord blood stem cell cryopreservation bank includes a stem cell preservation bag 13; it also includes a bank body 3 and a storage box 14 for storing the stem cell preservation bag 13; the bank body 3 is provided with at least one vertically movable frame 5, and the movable frame 5 is provided with a vertically movable push box 8; liquid nitrogen storage tanks 2 are distributed on both sides of the movable frame 5 inside the bank body 3; the back of the liquid nitrogen storage tank 2 is connected to the liquid nitrogen storage tank 12 through a nitrogen replenishment pipe 204; the front of the bank body 3 is provided with a placement cavity 11 for placing the storage box 14; the upper end of the liquid nitrogen storage tank 2 is provided with a liftable and openable box cover 202, and the bottom of the box cover 202 is provided with a tray 203 for placing the storage box 14; the push box 8 is open on the front and both sides; the top surface of the inner cavity of the push box 8 is provided with a vertically movable and horizontally movable push rod 20; the two ends of the push rod 20 are provided with clamping rods 27 for clamping the storage box 14; the back of the push box 8 is provided with a push plate 38.

[0047] Specifically, the stem cell preservation bag 13 is a soft bag, which is existing technology. This type of bag, used for storing stem cells, has high strength and excellent sealing performance, and can withstand low-temperature freezing. The outer wall of the storage unit 3 is generally filled with insulating cotton for thermal insulation. The storage box 14 is a cubic or cuboid block structure, including an upper and lower cover. After the stem cell preservation bag 13 is placed into the storage box 14, the upper and lower covers are tightened. Positioning grooves 15 are formed on the four side walls of the storage box 14, and these grooves 15 engage with the positioning protrusions 28 on the clamping rod 27. The storage box 14 needs to be made of a material that can withstand low-temperature freezing, and can be made of transparent material; the storage box 14 can be labeled or numbered; the label or number generally corresponds to the liquid nitrogen storage box 2; multiple movable racks 5 can be arranged in a horizontal array within the storage facility, and each movable rack 5 can control two rows of liquid nitrogen storage boxes 2; the liquid nitrogen storage boxes 2 are distributed in a vertical wall-like manner within the storage facility, that is, in a rectangular array in a vertical plane; each liquid nitrogen storage box 2 stores only one storage box 14, therefore the liquid nitrogen storage box 2 can be set very small; at the same time, each liquid nitrogen storage box 14 can be set to a very small size. The storage tank 2 has a nitrogen replenishment pipe 204 on one side. A solenoid valve and a check valve can be installed on the nitrogen replenishment pipe 204. The nitrogen replenishment pipe 204 is connected to the liquid nitrogen storage tank 12. When the liquid nitrogen in a liquid nitrogen storage tank 2 decreases, the solenoid valve is opened to replenish the liquid nitrogen. The push box 8 can move up and down within the moving frame 5. Coordinating with the longitudinal movement of the moving frame 5, it can be positioned to the location of each liquid nitrogen storage tank 2. The lid 202 of the liquid nitrogen storage tank 2 is raised and lowered by a fourth lifting device 201. The fourth lifting device 201 can be an electric push rod or an electromagnetic push rod. The push box 8 moves to... After the liquid nitrogen storage box 2 is positioned, the fourth lifter 201 controls the opening of the box cover 202, and the clamping rod 27 inside the push box 8 extends to remove the storage box 14 on the tray 203, or to send the storage box 14 from the push box 8 into the liquid nitrogen storage box 2; when it is necessary to remove the storage box 14, the push box 8 moves to the outlet position of the placement cavity 11, and the pusher plate 38 pushes the storage box 14 into the placement cavity 11. At the same time, the conveyor belt mechanism 10 inside the placement cavity 11 conveys the storage box 14 to the entrance position of the placement cavity 11, making it convenient for the staff to take it away.

[0048] like Figure 10 As shown, in a preferred embodiment, the upper end of the push rod 20 is provided with a vertical shaft 29; the vertical shaft 29 passes through the slide 19; the slide 19 is movably mounted on the lead screw 31; the lead screw 31 is mounted on the top of the push box 8; the lead screw 31 is driven to rotate by the drive motor 35; a third lifter 30 is provided on the slide 19; the third lifter 30 drives the vertical shaft 29 to move up and down.

[0049] Specifically, an installation cavity can be partitioned above the inner cavity of the push box 8. The drive mechanism of the push rod 20 is entirely located in the installation cavity. A horizontal groove is opened on the partition, and the vertical shaft 29 passes through the horizontal shaft and connects to the push rod 20. A through hole is opened on the slide 19, and the upper end of the vertical shaft 29 passes through the through hole and connects to the third lifter 30. The third lifter 30 can be an electric push rod or an electromagnetic push rod. Both ends of the slide 19 have threaded holes that mate with the lead screw 31. The threaded holes are connected to the lead screw 31. When the lead screw 31 rotates, it drives the slide 19 to move axially. The third lifter 30 then drives the vertical shaft 29 to move up and down.

[0050] like Figure 11 As shown, in a preferred embodiment, two lead screws 31 are arranged parallel to each other; the slide block 19 is mounted on the lead screws 31 at both ends; a driven gear 33 is provided at the end of the lead screw 31; the driven gears 33 mesh with each other through a transmission gear 32; one of the driven gears 33 meshes with a drive gear 34 on the motor shaft of the drive motor 35.

[0051] Specifically, the slide 19 is driven to move by two lead screws 31, which can improve the movement stability and positioning accuracy of the slide 19; the drive motor 35 is installed in the mounting cavity above the push box 8.

[0052] like Figure 13-14 As shown, in a preferred embodiment, the push rod 20 is provided with a rotatable spline sleeve 26, and a spline shaft 24 is sleeved inside the spline sleeve 26. The front end of the spline shaft 24 is connected to the clamping rod 27, and the rear end is rotatably connected to the first telescopic rod 21. The outer wall of the spline sleeve 26 is provided with threads and a threaded sleeve 25 is sleeved on it. The threaded sleeve 25 is connected to the second telescopic rod 22. The second telescopic rod 22 drives the threaded sleeve 25 to move axially, thereby driving the spline sleeve 26 to rotate. The spline sleeve 26 drives the spline shaft 24 to rotate, thereby driving the clamping rod 27 to rotate. The extension and retraction of the first telescopic rod 21 drives the extension and retraction of the spline shaft 24, thereby driving the clamping rod 27 to open and close.

[0053] Specifically, the inner wall of the spline sleeve 26 has a spline groove; the spline groove mates with the spline protrusion on the outer wall of the spline shaft 24; the outer wall of the spline sleeve 26 is threaded for mates with the threaded sleeve 25; the spline sleeve 26 is axially limited and can rotate; the side wall of the threaded sleeve 25 is connected to the second telescopic rod 22, and the threaded sleeve 25 is driven to move axially through the second telescopic rod 22, thereby driving the spline sleeve 26 to rotate, and the spline sleeve 26 drives the spline shaft 24 to rotate. One end of the spline shaft 24 is connected to the clamping rod 27, and the other end is connected to the first telescopic rod 21 through the rotary joint 23; the first telescopic rod 21 drives the spline shaft 24 to move axially, thereby achieving the clamping and closing of the two clamping rods 27; wherein, the first telescopic rod 21 and the second telescopic rod 22 can be electric push rods or electromagnetic push rods.

[0054] like Figure 9 As shown, in a preferred embodiment, the pusher plate 38 is connected to the third telescopic rod 39; the third telescopic rod 39 is installed at the bottom of the pusher box 8. An electromagnet can be installed on the pusher plate 38 to cooperate with the iron sheet embedded in the inner wall of the storage box 14 to attract it, thereby realizing the function of pushing and pulling the storage box 14.

[0055] Specifically, the pusher plate 38 is located below the back of the inner cavity of the pusher box 8, and the third telescopic rod 39 can be installed at the bottom of the pusher box 8; the pusher plate 38 is driven to move back and forth by the third telescopic rod 39, and the pusher plate 38 is used to push the storage box 14 in the pusher box 8 into the placement cavity 11.

[0056] like Figure 8 As shown, in a preferred embodiment, the storage box 14 has positioning grooves 15 distributed on its side wall; the clamping surface of the clamping rod 27 is provided with positioning protrusions 28 for engaging with the positioning grooves 15.

[0057] Specifically, at least two sets of positioning grooves 15 are distributed on the same side wall, generally four sets of positioning grooves 15. Each set of positioning grooves 15 is used to cooperate with the clamping rod 27. Each set of positioning grooves 15 is provided with two positioning holes. By cooperating with the positioning holes through the two positioning protrusions 28 on the clamping rod 27, it can be ensured that the clamping rod 27 can clamp the storage box 14, and even lift the storage box 14 by the rise of the push rod 20.

[0058] like Figure 12 As shown, in a preferred embodiment, lifting wheels 17 are provided on the upper and lower side walls of the push box 8; wherein the lower lifting wheel 17 is connected to the gear transmission mechanism 173 through the belt transmission mechanism 171; the gear transmission mechanism 173 is connected to the lifting motor 172.

[0059] Specifically, there are at least eight lifting wheels 17, two on each of the upper sides of the push box 8 and two on each of the lower sides; the opposite pairs are connected to each other by axles; the lifting wheels 17 are suitable for using gears, and a guide rail for moving the lifting wheels 17 is installed on the moving frame 5, the guide rail surface being a rack surface; the lifting wheels 17 are driven to rotate by a belt drive mechanism 171, which is driven to rotate by a gear drive mechanism 173, which is driven to rotate by a lifting motor 172; the belt of the belt drive mechanism 171 can be a toothed belt.

[0060] like Figure 6 As shown, in a preferred embodiment, the upper and lower ends of the movable frame 5 are connected to the lead screw mechanism 4; the tail ends of the lead screw mechanism 4 are connected to each other through the belt drive mechanism 1.

[0061] Specifically, the movable frame 5 is rectangular in shape with a hollow cavity inside, used for pushing the box 8 to move up and down. The movable frame 5 has slots on three sides for the storage box 14 to pass through. Threaded holes are opened at the top and bottom ends for cooperating with the lead screw mechanism 4 and driving the movement through the lead screw mechanism 4. At the same time, sliding shaft holes are opened on both sides of the threaded holes for installing sliding shafts, thereby improving the movement stability of the movable frame 5.

[0062] like Figure 2 As shown, in a preferred embodiment, the top and bottom surfaces of the placement cavity 11 are provided with a conveyor belt mechanism 10; a lifting door 9 is provided at the outlet of the placement cavity 11; the lifting door 9 is connected to the first lifter 7.

[0063] Specifically, the conveyor belt mechanism 10 can rotate in both directions to input the storage box 14 into the push box 8, or cooperate with the pusher plate 38 in the push box 8 to transfer the storage box 14 in the push box 8 into the placement cavity 11. The first lifter 7 can be an electric push rod or an electromagnetic push rod.

[0064] like Figure 1 As shown, in a preferred embodiment of the above, the front of the library body 3 is provided with a display screen 36 and operation buttons 37.

[0065] Specifically, the display screen 36 is used to display the storage number or storage quantity, and the operation button 37 is used to store or retrieve the data.

[0066] The specific working principle of this invention is as follows:

[0067] like Figure 1-2 As shown, the storage box 14 containing the stem cell preservation bag 13 is placed into the placement cavity 11. The first lifter 7 drives the lifting door 9 to rise, and the conveyor belt mechanism 10 rotates in the forward direction to transfer the storage box 14 into the push box 8. At the same time, the pusher plate 38 in the push box 8 can retract to make room, or an electromagnet can be installed on the pusher plate 38 to attract the storage box 14 and retract it, bringing the storage box 14 into the push box 8.

[0068] like Figure 5-14 As shown, the push box 8 moves up and down, while the moving frame 5 moves back and forth, so that the push box 8 moves to the corresponding liquid nitrogen storage box 2 position. The fourth lifter 201 rises, causing the box cover 202 to open, revealing the tray 203. The push rod 20 of the push box 8 is at the top position and is moved to the rear of the storage box 14 by the slide 19. Then the push rod 20 descends to the set position, and the clamping rod 27 extends to clamp the storage box 14. The push rod 20 moves the storage box 14, so that the storage box 14 is placed into the tray 203. Then the push rod 20 returns to its original position. The fourth lifter 201 descends, causing the tray 203 to descend into the liquid nitrogen and freeze.

[0069] When storage box 14 needs to be removed, the fourth lifter 201 rises, causing the box cover 202 to open and reveal the tray 203. The push rod 20 of the push box 8 extends the clamping rod 27 to hold the storage box 14 and drag the storage box 14 to move it into the push box 8. The fourth lifter 201 descends, causing the box cover 202 to close. The push box 8 moves up or down, and at the same time, the moving frame 5 moves forward so that the front outlet of the push box 8 is aligned with the position of the placement cavity 11. The first lifter 7 drives the lifting door 9 to rise, and the pusher plate 38 pushes forward to push the storage box 14 into the placement cavity 11. At the same time, the conveyor belt mechanism 10 rotates in the opposite direction to transfer the storage box 14 into the push placement cavity 11 for easy removal by the staff.

[0070] Since the liquid nitrogen storage tank 2 consumes some liquid nitrogen each time it is opened, a nitrogen replenishment pipe 204 is provided on the back of the liquid nitrogen storage tank 2. When the temperature sensor detects that the temperature inside the liquid nitrogen storage tank 2 is too high, it indicates that the amount of liquid nitrogen is insufficient, so the liquid nitrogen storage tank 12 automatically replenishes the liquid nitrogen storage tank 2.

[0071] This invention employs a storage method where each liquid nitrogen storage box 2 corresponds to each stem cell storage bag 13, facilitating accurate digital management. Through the cooperation of the push box 8 and the mobile rack 5, fully automated and rapid storage and retrieval of umbilical cord blood stem cells are achieved. Each umbilical cord blood stem cell cryopreservation bag is stored in a single small liquid nitrogen storage box 2, which improves the preservation quality and enables rapid identification and accurate storage management of umbilical cord blood stem cells.

[0072] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0073] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only for the purpose of helping to understand the method and core ideas of the present invention.

[0074] The above description is only a preferred embodiment of the present invention. It should be noted that due to the limitations of written expression, there are objectively infinite specific structures. For those skilled in the art, several improvements, modifications or changes can be made without departing from the principle of the present invention, and the above technical features can be combined in an appropriate manner. These improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without modification, should all be considered within the scope of protection of the present invention.

Claims

1. A cryopreservation bank for umbilical cord blood stem cells, comprising a stem cell preservation bag (13); characterized in that, It also includes a storage body (3) and a storage box (14) for storing stem cell preservation bags (13); the storage body (3) is provided with at least one vertically movable frame (5), and the movable frame (5) is provided with a vertically movable push box (8); liquid nitrogen storage tanks (2) are distributed on both sides of the movable frame (5) in the storage body (3); the back of the liquid nitrogen storage tank (2) is connected to the liquid nitrogen storage tank (12) through a nitrogen replenishment pipe (204); the front of the storage body (3) is provided with a place to put the storage box (14). The liquid nitrogen storage box (2) is equipped with a liftable lid (202) at the top and a tray (203) for placing the storage box (14) at the bottom of the lid (202); the push box (8) is open on the front and sides; the push box (8) is equipped with a push rod (20) that can be lifted up and down and moved laterally on the top surface of the inner cavity; the push rod (20) is equipped with clamping rods (27) at both ends for clamping the storage box (14); the push box (8) is equipped with a push plate (38) on the back. A vertical shaft (29) is provided at the upper end of the push rod (20); the vertical shaft (29) passes through the slide (19); the slide (19) is movably mounted on the lead screw (31); the lead screw (31) is mounted on the top of the push box (8); the lead screw (31) is driven to rotate by a drive motor (35); a third lifter (30) is provided on the slide (19); the third lifter (30) drives the vertical shaft (29) to move up and down. Two lead screws (31) are arranged parallel to each other; the slide (19) is mounted on the lead screws (31) at both ends; a driven gear (33) is provided at the end of the lead screw (31); the driven gears (33) mesh with each other through a transmission gear (32); one of the driven gears (33) meshes with the drive gear (34) on the motor shaft of the drive motor (35); The pusher plate (38) is connected to the third telescopic rod (39); the third telescopic rod (39) is installed at the bottom of the pusher box (8); The storage box (14) has positioning grooves (15) distributed on its side wall; the clamping surface of the clamping rod (27) is provided with positioning protrusions (28) for engaging with the positioning grooves (15). The top and bottom surfaces of the placement cavity (11) are provided with a conveyor belt mechanism (10); a lifting door (9) is provided at the outlet of the placement cavity (11); the lifting door (9) is connected to the first lifter (7); The push rod (20) is provided with a rotatable spline sleeve (26), which is axially limited and can rotate; a spline shaft (24) is sleeved inside the spline sleeve (26), the front end of the spline shaft (24) is connected to a clamping rod (27), and the rear end is rotatably connected to a first telescopic rod (21). One end of the spline shaft (24) is connected to the clamping rod (27), and the other end is connected to the first telescopic rod (21) through a rotary joint (23); the spline shaft (24) is rotatably connected to a first telescopic rod (21). The outer wall of the key sleeve (26) is provided with threads and a threaded sleeve (25) is fitted on it; the threaded sleeve (25) is connected to the second telescopic rod (22); the second telescopic rod (22) drives the threaded sleeve (25) to move axially, thereby driving the spline sleeve (26) to rotate, the spline sleeve (26) drives the spline shaft (24) to rotate, thereby driving the clamping rod (27) to rotate; the first telescopic rod (21) extends and retracts to drive the spline shaft (24) to extend and retract, thereby driving the clamping rod (27) to open and close.

2. The umbilical cord blood stem cell cryopreservation bank according to claim 1, characterized in that, The push box (8) is provided with lifting wheels (17) on the upper and lower side walls; the lower lifting wheel (17) is connected to the gear transmission mechanism (173) through the belt transmission mechanism (171); the gear transmission mechanism (173) is connected to the lifting motor (172).

3. The umbilical cord blood stem cell cryopreservation bank according to claim 1, characterized in that, The upper and lower ends of the movable frame (5) are connected to the lead screw mechanism (4); the tail ends of the lead screw mechanism (4) are connected to each other through the belt drive mechanism (1).

4. The umbilical cord blood stem cell cryopreservation bank according to claim 1, characterized in that, The front of the storage unit (3) is equipped with a display screen (36) and operation buttons (37).

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