A noodle pest control agent using plant-derived composites and its method and application

Abstract

The present invention provides a noodle pest control agent using a plant-derived composite material, and a method and application thereof. The present invention uses plant-derived essential oil as a repellent, and adjusts different concentration gradients to conduct a repellent experiment on rice weevils, and obtains the type and concentration range of essential oils with a good repellent effect on rice weevils; screens attractants to obtain the best attractant concentration range for rice weevils; and finally obtains the best repellent and attractant for combined use to achieve pest control during noodle storage. The method is safe, green, efficient, sustainable, simple and easy to implement, and can be flexibly applied to the outer layer of current noodle factories and household noodle packaging, overcoming the shortcomings of the current physical or chemical insect control means that the storage environment is strict and the cost is high, while also avoiding the residual of the agent, filling the gap of pest control in noodles using plant-derived essential oils.

Description

Technical Field

[0001] The invention belongs to the technical field of pest control, and relates to a method for controlling rice weevils in noodles, and in particular to a noodle pest control agent using a plant-based composite, and a method and application thereof. Background Art

[0002] Almost 40% of wheat grown in Asia is consumed in the form of noodles, and noodles are the most consumed pasta product in my country, with the characteristics of long-lasting storage and convenient consumption. However, the problem of insect pests in noodles has always existed in the industry and is difficult to solve. The processing processes such as dough mixing, rolling, and drying have destroyed or removed most of the insect eggs in the noodles, but the problem of insect pests in the finished noodles is still severe, among which rice weevils are the most common. As a kind of borer pest, rice weevils can not only feed on raw grain crops such as wheat and rice, causing losses in grain quantity and quality and creating conditions for the occurrence of flour-feeding pests such as red flour beetles and saw-toothed grain beetles, but also can bore into the noodles through the pores on the packaging to feed and reproduce on a large scale. According to the investigation, a noodle manufacturer received as many as 409 complaints about noodle infestation in two years, with an average of nearly 200 cases per year. Therefore, when preventing and controlling pests, it is necessary not only to consider the growth of endogenous pests, but also to avoid the invasion of exogenous pests during storage and transportation. Pests feed on noodles mainly by identifying volatile components in noodles, which are then diffused through packaging materials. These diffused volatile components attract pests to feed, which in turn causes large-scale reproduction of pests in noodles. In response to pest contamination, domestic and foreign scholars have adopted physical and chemical methods to control it, mainly including gas conditioning (publication number: CN115644153A), temperature control (publication number: CN215380045U, CN203980575U), inert powder (publication number: CN115363007A) and fumigation (publication number: CN109362365B, CN112237204A) and other means. Existing research shows that controlled atmosphere (publication number: CN217695055U, CN111820202B) storage has high requirements for the airtightness of the storage environment, and the grain cooler (publication number: CN217275129U, CN216650565U) temperature control method has high requirements for the thermal insulation of the storage environment and high temperature control costs; chemical fumigation mostly uses phosphine, aluminum phosphide (publication number: CN109362365B, CN209542617U, CN112237204A) and other agents to treat the storage environment, however, these agents are mostly used for empty warehouse pest control or raw grain pest control, and agent residue is also a major problem.

[0003] In summary, the current physical and chemical methods for controlling pests in raw grains are not suitable for direct application in the prevention and control of pests in noodles, and there are also problems such as increased costs and pesticide residues. Summary of the invention

[0004] In view of the above technical problems, the present invention provides a noodle pest control agent using a plant-derived composite, and a method and application thereof. The method is safe, green, efficient, sustainable, simple and easy to implement, and can be flexibly applied to the outer layer of the current noodle factory and household noodle packaging, overcoming the shortcomings of the current physical or chemical insect control methods that have strict requirements on the storage environment and high costs, while also avoiding the residual of the agent, filling the gap in the use of plant-derived essential oils for pest control in noodles, and greatly improving the efficiency of pest control.

[0005] Note that the description of these objectives does not prevent the existence of other objectives. One embodiment of the present invention does not need to achieve all of the above objectives. Objectives other than the above objectives can be extracted from the description of the specification, drawings, and claims.

[0006] The present invention adopts plant-derived essential oils (citrus essential oils, clove essential oils, lemon essential oils, trans-cinnamaldehyde, eugenol, etc.) as repellents, and adjusts different concentration gradients to carry out repellent experiments on rice weevils, and obtains the essential oil types and concentration ranges with good repellent effects on rice weevils; screens attractants (vanillin, eicosane, dichloromethane, etc.), and adjusts different concentration gradients, carries out luring experiments on rice weevils, and obtains the best attractant concentration range for rice weevils; and finally obtains the best repellent and attractant combined use, and realizes the prevention and control of pests in the storage process of noodles. This method is safe, green, efficient, sustainable, simple and easy to implement, and can be flexibly applied to the outer layer of the current noodle factory and the household noodle packaging, and overcomes the shortcomings of the current physical or chemical insect-proof means that the storage environment is strict and the cost is high, and also avoids the residual of the agent, and fills the blank of pest control in noodles using plant-derived essential oils.

[0007] The present invention achieves the above technical objectives through the following technical means.

[0008] A method for controlling noodle pests using a plant-derived compound comprises the following steps:

[0009] Step S1, using plant-derived essential oils as repellents to screen out the types and concentration ranges of essential oils with the best repellent effect on rice weevils, and using plant-derived compounds as attractants to screen out the types and concentration ranges of plant-derived compounds with the best attractant effect on rice weevils;

[0010] Step S2, using the repellent screened in step S1 to control rice weevils in noodles, to obtain the screened optimal repellent and its concentration range for controlling rice weevils in noodles, and using the attractant screened in step S1 to control rice weevils in noodles, to obtain the screened optimal attractant and its concentration range for controlling rice weevils in noodles;

[0011] Step S3, combining the repellent and the attractant in the optimal concentration range in step S2 for controlling rice weevils in noodles to obtain an optimal combination of repellent and attractant;

[0012] Step S4, applying the optimal combination of repellent and attractant obtained in step S3 to control rice weevils in noodles, wherein the repellent is placed on the outer surface of the noodle package and the attractant is placed away from the noodles.

[0013] In the above scheme, the repellents screened in step S1 are clove essential oil, lemon essential oil, and trans-cinnamaldehyde.

[0014] Preferably, the concentrations of the clove essential oil, lemon essential oil and trans-cinnamaldehyde are all 5 mg / ml.

[0015] In the above scheme, the attractants screened in step S1 are vanillin dichloromethane complex and eicosane dichloromethane complex.

[0016] Preferably, the concentration of the vanillin dichloromethane complex is 1 mg / ml; the concentration of the eicosane dichloromethane complex is 1 mg / ml to 10 mg / ml.

[0017] In the above scheme, the optimal repellents for controlling rice weevils in noodles in step S2 are clove essential oil and trans-cinnamaldehyde, and the concentrations of clove essential oil and trans-cinnamaldehyde are both 5 mg / ml.

[0018] In the above scheme, the optimal attractants for controlling rice weevils in noodles in step S2 are vanillin dichloromethane complex and eicosane dichloromethane complex; the concentration of the vanillin dichloromethane complex is 1 mg / ml; the concentration of the eicosane dichloromethane complex is 5 mg / ml.

[0019] In the above scheme, the optimal repellent and attractant combination obtained in step S3 is: a combination of 5 mg / ml clove essential oil and 1 mg / ml vanillin dichloromethane complex, a combination of 5 mg / ml clove essential oil and 5 mg / ml eicosane dichloromethane complex, a combination of 5 mg / ml trans-cinnamaldehyde and 1 mg / ml vanillin dichloromethane complex, and a combination of 5 mg / ml trans-cinnamaldehyde and 5 mg / ml eicosane dichloromethane complex.

[0020] Preferably, the optimal repellent and attractant combination obtained in step S3 is: 5 mg / ml trans-cinnamaldehyde and 5 mg / ml eicosanoid dichloromethane complex.

[0021] A noodle pest control agent using a plant-derived composite is prepared according to the noodle pest control method using a plant-derived composite. The noodle pest control agent can be flexibly made into various forms such as an insect repellent box and an insect repellent card.

[0022] An application of the method for controlling noodle pests using the plant-derived composite, wherein the application of the method in controlling noodle pests mainly includes the following representative applications:

[0023] In a factory environment, an insect repellent box is placed on the outer layer of the noodle storage position, which contains essential oil with a strong repellent effect after being optimized by the method of the present invention; at the same time, insect attractant boxes are placed around the noodle storage position away from the noodle storage position, which contain a compound with a strong attractant effect after being optimized by the method of the present invention; in a warehouse environment, insect repellent cards containing essential oil with a strong repellent effect after being optimized by the method of the present invention can also be posted on the outer layer of the noodle packaging, and insect attractant boxes containing the compound with a strong attractant effect after being optimized by the method of the present invention can be placed around the storage environment; in a home environment, the above-mentioned insect repellent cards are placed close to the noodles, and the insect attractant cards containing the compound with a strong attractant effect after being optimized by the method of the present invention are placed away from the noodles, so as to prevent and control pests in the home.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) In the past, plant essential oils were used for pest control, mostly using a single essential oil, which had limited pest control effects. The present invention, however, is the first to utilize a plant-derived composite material for the control of rice weevils on noodles. Plant-derived essential oils are used to control rice weevils on noodles based on a "push-pull" strategy. The optimal repellent and its concentration range and attractant and its concentration range are selected and applied to the control of rice weevils on noodles to obtain the optimal combination of repellent and attractant, thereby making the use of plant essential oils more refined, greatly improving the efficiency of the use of attractants and repellents, and achieving excellent insect control effects.

[0026] (2) Pest control measures such as controlled atmosphere and temperature control require extremely high storage requirements, such as thickening walls, laying ventilation systems and installing large grain coolers. Compared with controlled atmosphere and temperature control, the present invention does not require extremely high storage requirements, and therefore will not increase the economic burden on manufacturers and consumers.

[0027] (3) Although inert powder, phosphine fumigation and other methods are highly effective, they cannot be directly used in noodles because noodles need to be eaten directly. In contrast, the plant-derived essential oil of the present invention is simple to extract, low in price, has a long effective period and is non-toxic and harmless, making it a more suitable insect-proof method for noodles.

[0028] In summary, the present invention avoids the extremely high storage conditions required by means of gas conditioning, temperature control, etc., solves the problem that means such as inert powder and fumigation cannot be directly used for noodles, screens the concentration and combination of essential oils and actually applies them to noodle pest control, and is a green, efficient and sustainable noodle pest control solution.

[0029] Note that the description of these effects does not prevent the existence of other effects. One embodiment of the present invention does not necessarily have all of the above effects. Effects other than the above can be clearly seen and extracted from the description of the specification, drawings, claims, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 Comparison of the attractant effects of different concentrations of vanillin-dichloromethane complexes on rice weevils. Figure 1 (1) is a graph showing the reaction rate of rice weevils to vanillin dichloromethane complexes with different concentrations; Figure 1 (2) is a graph showing the attraction rate of rice weevils to vanillin-dichloromethane complexes at different concentrations; Figure 1 (3) is a graph showing the relative attraction rate of rice weevils to vanillin-dichloromethane complexes at different concentrations; Figure 1 (4) is a graph showing the selectivity coefficients of rice weevils for vanillin-dichloromethane complexes at different concentrations.

[0031] Figure 2 Comparison of the attractant effects of different concentrations of eicosanoid-dichloromethane complex on rice weevils. Figure 2 (1) is a graph showing the reaction rate of rice weevils to different concentrations of eicosane-dichloromethane complex; Figure 2 (2) is a graph showing the attraction rate of rice weevils to different concentrations of eicosane-dichloromethane complex; Figure 2 (3) is a graph showing the relative attraction rate of rice weevils to different concentrations of eicosane-dichloromethane complex; Figure 2 (4) is a graph showing the selectivity coefficient of rice weevils for different concentrations of eicosane-dichloromethane complexes.

[0032] Figure 3 Comparison of the insect control effects of different groups of repellents on noodles. Figure 3 (1) is a graph showing the reaction rate, attraction rate, and relative attraction rate of rice weevils to noodles after the repellent was applied; Figure 3 (2) is a graph showing the selection coefficient of rice weevils for noodles after the action of repellents, in which A: control group, noodles-blank; B: 5 mg / ml clove essential oil and noodles-blank; C: 5 mg / ml lemon essential oil and noodles-blank; D: 5 mg / ml trans-cinnamaldehyde and noodles-blank.

[0033] Figure 4 Comparison of the insect control effects of different groups of attractants on noodles. Figure 4 (1) is a graph showing the reaction rate, attraction rate, and relative attraction rate of rice weevils to noodles after the attractant was applied; Figure 4(2) is a graph showing the selection coefficient of rice weevils for fine noodles after the action of attractants, in which A: control group, fine noodles-blank; B: fine noodles-1 mg / ml vanillin dichloromethane complex; C: fine noodles-1 mg / ml eicosane dichloromethane complex; D: fine noodles-5 mg / ml eicosane dichloromethane complex; E: fine noodles-10 mg / ml eicosane dichloromethane complex.

[0034] Figure 5 Comparison of the insect control effects of different push-pull combinations on noodles. Figure 5 (1) is a graph showing the reaction rate, attraction rate, and relative attraction rate of rice weevils to noodles after different combinations of push and pull schemes; Figure 5 (2) is a graph showing the selectivity coefficient of rice weevils for fine noodles after the action of different combinations of push-pull schemes, in which A: control group, fine noodles-blank; B: 5 mg / ml clove oil and fine noodles-1 mg / ml vanillin dichloromethane complex; C: 5 mg / ml clove oil and fine noodles-5 mg / ml eicosane dichloromethane complex; D: 5 mg / ml trans-cinnamaldehyde and fine noodles-1 mg / ml vanillin dichloromethane complex; E: 5 mg / ml trans-cinnamaldehyde and fine noodles-5 mg / ml eicosane dichloromethane complex. DETAILED DESCRIPTION

[0035] Unless otherwise specified, the terms used in the present invention generally have the meanings commonly understood by those of ordinary skill in the art. The present invention is further described in detail below in conjunction with specific embodiments and with reference to data, but the protection scope of the present invention is not limited thereto.

[0036] Example 1

[0037] Evenly apply 100 μl of citrus essential oil, clove essential oil, lemon essential oil, trans-cinnamaldehyde, and eugenol at concentrations of 0.1 mg / ml, 1 mg / ml, and 5 mg / ml on 1 / 2 of a 9 cm round filter paper, and evenly apply 100 μl of n-hexane solution on the other half of the filter paper. After natural drying, use transparent tape to stick them together again, and use handmade white glue to stick the treated filter paper tightly to the bottom of a clean culture dish (pay attention to stick it firmly without leaving gaps to prevent the test insects from drilling under the filter paper and affecting observation). After the culture dish is naturally dried, release 20 rice weevils, and calculate the repellency rate after observing for a period of time. It is found that 5 mg / ml of clove essential oil, lemon essential oil, and trans-cinnamaldehyde have a good repellency effect, as shown in Table 1. The results showed that the repellent effect of citrus essential oil increased in turn at the three concentrations of 0.1mg / ml, 1mg / ml, and 5mg / ml. The repellent effect at the two concentrations of 0.1mg / ml and 1mg / ml was not obvious, only at level I-II; at a concentration of 5mg / ml, the repellent level was level IV and maintained for 8h, but it quickly dropped to level III after 8h, and the repellent effect was limited. The repellent effect of clove essential oil increased significantly with concentration. The repellent level at 0.1mg / ml and 1mg / ml was below level III, and the repellent effect at 5mg / ml could reach level V and could be maintained at level IV for more than 16h. Lemon essential oil had a good repellent effect at low concentrations, reaching about level III at a concentration of 0.1mg / ml; as the concentration increased to 5mg / ml, the repellent level of lemon essential oil would reach level V and still maintain a level III repellent effect after 16h. Trans-cinnamaldehyde has a small repellent effect at low concentrations. At 0.1mg / ml and 1mg / ml, the repellent level is at level I-II. At 5mg / ml, the repellent effect is significantly enhanced, reaching level V, but it immediately drops to level IV after 8h, and to level III after 16h. Eugenol has a general repellent effect at low concentrations. When the concentration reaches 5mg / ml, the repellent effect can reach level IV, but its repellent effect decreases significantly over time, and the repellent level is level II after 16h.

[0038] In the above embodiment, the preparation scheme of repellents of different concentrations is: different plant essential oils are used as solutes and n-hexane is used as solvent to prepare repellents of different concentrations; the calculation method of the repellent effect is: 20 adult insects are inoculated into each culture dish, and the number of test insects distributed on both sides is counted at 2, 4, 8, and 16 hours after inoculation, and the repellent rate is calculated:

[0039] Repellency rate = (number of control anaplasma - number of treated anaplasma) / number of control anaplasma × 100%.

[0040] The grading standard of repellent activity is as follows: Grade 0, no repellent activity; Grade I, repellent rate 0-20.0%; Grade II, repellent rate 20.1%-40.0%; Grade III, repellent rate 40.1%-60.0%; Grade IV, repellent rate 60.1%-80.0%; Grade V, repellent rate 80.1%-100%.

[0041] Example 2

[0042] Different concentrations (0.1mg / ml, 1mg / ml, 5mg / ml, 10mg / ml, 20mg / ml) of vanillin dichloromethane and eicosane dichloromethane complex were numbered J1-J10, and air was used as a blank control, numbered J0. The design combinations were J0-J1, J0-J2, J0-J3, J0-J4, J0-J5, J0-J6, J0-J7, J0-J8, J0-J9, and J0-J10. According to the above combination, 100μl of the compound was applied on 1 / 2 of a 9cm round filter paper and placed in a Y-type olfactometer odor source bottle. Air was used as a blank control. After waiting for 10 minutes for the odor to be emitted, the air pump was turned on and 20 rice weevils were released for the luring experiment. The luring effect was observed. It was found that 1mg / ml of vanillin dichloromethane complex and 1, 5, and 10mg / ml of eicosane dichloromethane complex had the best luring effect. The results are shown in the figure. Figure 1 , Figure 2 shown. Figure 1 (1) shows that the reaction rate of rice weevils to vanillin dichloromethane complex at different concentrations was slightly different, basically maintained at around 55%. After the concentration increased to 5 mg / ml, the reaction rate decreased and fluctuated around 50%. Figure 1 (2) and (3) show that when the vanillin concentration is 0, the relative attraction rate of pure dichloromethane to rice weevils is 2.5%, which is almost ineffective. However, as the vanillin concentration increases from 0.1 mg / ml to 20 mg / ml, the attraction rate first increases and then decreases. The attraction rate is the highest at a vanillin concentration of 1 mg / ml, which is 40%. As the concentration continues to increase, the attraction rate decreases significantly. When the concentration is 20 mg / ml, the attraction rate drops to about 10%. Figure 1 (3) It can be seen that the relative attraction rate of vanillin dichloromethane complex to rice weevils at different concentrations has extremely significant differences, among which the relative attraction rate at a concentration of 1 mg / ml is the highest, which is 22.5%. Figure 1 (3) and (4) show that at higher concentrations, the relative attraction rate of vanillin to rice weevils is negative, showing a repellent effect, and the change in the selection coefficient under this condition also has the same trend. Figure 2 The results showed that different concentrations of dichloromethane and eicosane complex had different attracting effects on rice weevils, and the attracting effect first increased and then decreased with the increase of concentration. Figure 2 (1) shows that when the concentration of eicosane is 0, the reaction rate of rice weevils to pure dichloromethane is 57.50%. After adding different concentrations of eicosane, the reaction rate first decreases and then increases but basically maintains above 40%. Figure 2(2) showed that when the concentration of eicosane was 5 mg / ml, the rice weevil attraction rate was the highest, which was 52.5%, and when the concentration was 0.1 mg / ml, the lowest was 15%. Figure 2 (3) shows that after using different concentrations of eicosane-dichloromethane complex, the relative attraction rate of the attractant to rice weevils also has significant differences. When the attractant is pure dichloromethane, the relative attraction rate of the complex to rice weevils is almost 0; after adding eicosane, the relative attraction rate increases with the increase of its concentration; when the concentration of eicosane is 5 mg / ml, the relative attraction rate reaches the maximum value, which is 30%; when the concentration is 20 mg / ml, the relative attraction rate of rice weevils drops to a negative value, and the attractant's attraction to rice weevils disappears at this time. Figure 2 (4) showed that the selection coefficient had the same trend as the relative attraction rate and was higher at 1 mg / ml and 5 mg / ml, which were 0.42 and 0.40, respectively.

[0043] In the above embodiment, the preparation scheme of attractants of different concentrations is: different kinds of insect attractants are used as solutes and dichloromethane is used as solvent to prepare attractants of different concentrations; the evaluation method of the attractant effect is: using a Y-type olfactometer to detect the tendency behavior of rice weevils when stimulated by different odor sources in two directions at the same time, and taking the rice weevils crawling over 1 / 2 of the tube wall as the standard, the rice weevils' selection behavior is recorded. The test time is 9:00-17:00. After each group of experiments, the positions of the two tubes of the Y-type tube are turned around to eliminate the influence of the orientation. The results of attracting rice weevils with different odor sources are analyzed using indicators such as the attractant rate, reaction rate, relative attractant rate and selection coefficient:

[0044]

[0045]

[0046]

[0047]

[0048] If the selection coefficient is >0, it means that the rice weevil has a tendency to the corresponding odor source. The larger the value, the stronger the tendency, and the maximum value is 1. If the selection coefficient is <0, it means that the rice weevil has a negative tendency to the odor of the corresponding food source. If the selection coefficient is 0, it means that the rice weevil has no reaction to the odor of the corresponding food source.

[0049] Example 3

[0050] Screening of repellents in noodles:

[0051] Evenly apply 100 μl of 5 mg / ml clove essential oil on 1 / 2 of a 9 cm round filter paper, put the filter paper into the odor source bottle of the Y-type olfactometer and put in 10 g of 0.5 cm long noodle segments, with the other side as a blank control. After waiting for 10 minutes for the odor to be emitted, turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 3 As shown in Groups A and B in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 12.5%, -20% and -0.45, respectively.

[0052] Example 4: Evenly apply 100 μl of 5 mg / ml lemon essential oil on 1 / 2 of a 9 cm round filter paper, put the filter paper into the odor source bottle of a Y-type olfactometer and put in 10 g of 0.5 cm long noodle segments, with the other side as a blank control. After waiting for 10 minutes for the odor to be emitted, turn on the air pump and release 20 rice weevils to conduct an insect control experiment and observe the feeding tendency of rice weevils. Figure 3 As shown in Groups A and C in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 20%, -2.5% and -0.05, respectively.

[0053] Example 5

[0054] Evenly apply 100 μl of 5 mg / ml trans-cinnamaldehyde on 1 / 2 of a 9 cm round filter paper, put the filter paper into the odor source bottle of the Y-type olfactometer and put in 10 g of 0.5 cm long noodles, with the other side as a blank control. After waiting for 10 minutes for the odor to be released, turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 3 As shown in Groups A and D in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 22.5%, -17.5% and -0.28, respectively.

[0055] Depend on Figure 3 It can be seen that essential oils in different groups have different repellent effects on rice weevils that may pose an infection risk to noodles. Figure 3 (1) showed that the reaction rate of rice weevils was similar among different test groups, and the four AD groups fluctuated around 55% with a small fluctuation. Figure 3From Group A in (1) and (2), it can be seen that when no essential oil is used, rice weevils have a strong tendency to feed on noodles. At this time, the attraction rate, relative attraction rate and selection coefficient of noodles to rice weevils are at high values, namely 47.5%, 37.5% and 0.65 respectively. After using Group B, C and D essential oils around noodles, the attraction rate, relative attraction rate and selection coefficient of noodles to rice weevils are significantly reduced. Among them, Group B essential oil has the strongest repellent effect on rice weevils in noodles, making the attraction rate, relative attraction rate and selection coefficient of noodles to rice weevils the lowest values. Figure 3 (1) and (2) group B showed that it was 12.5%, -20%, and -0.45. The essential oil of group D also had a good effect on the repellency of rice weevils in noodles. The attraction rate, relative attraction rate and selection coefficient of rice weevils after acting on noodles were as follows: Figure 3 The results of group D in (1) and (2) were 22.5%, -17.5%, and -0.28. The effect of group C essential oil in repelling rice weevils in noodles was average, but it also had a certain repellent effect. After acting on noodles, the relative attraction rate and selection coefficient of rice weevils also dropped to negative values.

[0056] Example 6

[0057] Screening of attractants in noodles:

[0058] Evenly apply 100 μl of 1 mg / ml vanillin dichloromethane complex on 1 / 2 of a 9 cm round filter paper, place the filter paper in the odor source bottle of the Y-type olfactometer, and put 10 g of 0.5 cm long noodles in the odor source bottle on the other side. Wait for 10 minutes for the odor to be released, then turn on the air pump and release 20 rice weevils to conduct an insect control experiment and observe the feeding tendency of rice weevils. Figure 4 According to the calculations of Groups A and B in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 15%, -15% and -0.33, respectively. Figure 4 shown.

[0059] Example 7

[0060] Evenly apply 100 μl of 1 mg / ml eicosanoid-dichloromethane mixture on 1 / 2 of a 9 cm round filter paper, place the filter paper in the odor source bottle of the Y-type olfactometer, and put 10 g of 0.5 cm long noodles in the other odor source bottle. Wait for 10 minutes for the odor to be released, then turn on the air pump and release 20 rice weevils to conduct an insect control experiment and observe their feeding tendency. Figure 4 According to the calculations of Groups A and C in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 5%, -5% and -0.33, respectively. Figure 4 shown.

[0061] Example 8

[0062] Evenly apply 100 μl of 5 mg / ml dichloromethane mixture of eicosane on 1 / 2 of a 9 cm round filter paper, place the filter paper in the odor source bottle of the Y-type olfactometer, and put 10 g of 0.5 cm long noodles in the odor source bottle on the other side. Wait for 10 minutes for the odor to be released, then turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 4 According to the calculations of groups A and D in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 2.5%, -20% and -0.8 respectively. Figure 4 shown.

[0063] Example 9

[0064] Evenly apply 100 μl of 10 mg / ml dichloromethane mixture of eicosane on 1 / 2 of a 9 cm round filter paper, place the filter paper in the odor source bottle of the Y-type olfactometer, and put 10 g of 0.5 cm long noodles in the odor source bottle on the other side. After waiting for 10 minutes for the odor to be released, turn on the air pump and release 20 rice weevils to conduct an insect control experiment and observe the feeding tendency of rice weevils. Figure 4 According to the calculations of Groups A and E in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 0%, -5% and -1, respectively. Figure 4 shown.

[0065] Figure 4 It can be seen that different groups of attractants showed different degrees of control effects on rice weevils. Figure 4 (1) In group AE, it can be seen that compared with the reaction rate of rice weevils to noodles in group A without the use of attractants, the reaction rate of rice weevils in each test group BE was significantly reduced after the use of attractants, namely A>B>D>C>E. Figure 4 (1) shows that the rice weevils' attraction rate also decreased significantly. In group A, the attraction rate of noodles to rice weevils was 47.5%, and the other groups were lower than this value, among which group B was 15%, and group E was the lowest, which was 0%. The relative attraction rate of rice weevils also had significant differences among different groups, among which group D was the lowest, which was -20%, and group B was the second, which was -15%. In the other two experimental groups, the relative attraction rate of rice weevils to noodles did not drop as significantly as in groups B and D, but they were all negative values. Figure 4 (2) It can be observed that the selection coefficient and the relative attraction rate have the same changing trend. However, because the attraction rate of rice weevils in group E is 0, the selection coefficient of group E is the lowest after calculation, which is -1, followed by group D with a selection coefficient of -0.8.

[0066] Example 10

[0067] Push-pull application in noodles:

[0068] Evenly apply 100μl of 1mg / ml vanillin dichloromethane mixture on 1 / 2 of a 9cm round filter paper, put the filter paper into the odor source bottle of the Y-type olfactometer, evenly apply 100μl of 5mg / ml clove essential oil on the other 1 / 2 of the filter paper, put it into the odor source bottle on the other side and put in 10g of 0.5cm long noodles. Wait for 10 minutes for the smell to be released, then turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 5 According to the calculations of Groups A and B in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 7.5%, -15% and -0.51, respectively. Figure 5 shown.

[0069] Embodiment 11

[0070] Evenly apply 100μl of 5mg / ml eicosanoid-dichloromethane mixture on 1 / 2 of a 9cm round filter paper, place the filter paper in the odor source bottle of the Y-type olfactometer, evenly apply 100μl of 5mg / ml clove essential oil on the other 1 / 2 of the filter paper, then place it in the odor source bottle on the other side and put in 10g of 0.5cm long noodle segments, wait for 10 minutes for the odor to be released, turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 5 According to the calculations of Groups A and C in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 22.5%, -20% and -0.31, respectively. Figure 5 shown.

[0071] Example 12

[0072] Evenly apply 100μl of 1mg / ml vanillin dichloromethane mixture on 1 / 2 of a 9cm round filter paper, put the filter paper into the odor source bottle of the Y-type olfactometer, evenly apply 100μl of 5mg / ml trans-cinnamaldehyde on the other 1 / 2 of the filter paper, put it into the odor source bottle on the other side and put in 10g of 0.5cm long noodles. Wait for 10 minutes for the smell to be released, turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 5 According to the calculations of groups A and D in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 15%, -25% and -0.45, respectively. Figure 5 shown.

[0073] Embodiment 13

[0074] Evenly apply 100μl of 5mg / ml eicosanoid-dichloromethane mixture on 1 / 2 of a 9cm round filter paper, place the filter paper in the odor source bottle of the Y-type olfactometer, evenly apply 100μl of 5mg / ml trans-cinnamaldehyde on the other 1 / 2 of the filter paper, then place it in the odor source bottle on the other side and put in 10g of 0.5cm long noodle segments, wait for 10 minutes for the odor to be released, turn on the air pump and release 20 rice weevils for insect control experiments to observe the feeding tendency of rice weevils. Figure 5 According to the calculations of Groups A and E in (1) and (2), the attraction rate, relative attraction rate and selection coefficient of rice weevils to noodles decreased from 47.5%, 37.5% and 0.65 to 7.5%, -35% and -0.7, respectively. Figure 5 shown.

[0075] Figure 5 It can be seen that compared with the control group, the combination of essential oils in groups B, C, D, and E had a significant control effect on rice weevils in noodles, and the control effect of essential oils in groups D and E on rice weevils in noodles was stronger than any attractant or repellent used alone. Figure 5 (1) It can be seen that after using the essential oil of group B, the reaction rate of rice weevils to noodles decreased slightly, from 57.5% in group A to 30%. Although the reaction rate of rice weevils decreased due to the essential oil of group B, it still has a certain preventive effect on rice weevils. Figure 5 As can be seen from (1) and (2), after using the essential oil of group B, the attraction rate, relative attraction rate and selection coefficient of rice weevils decreased to 7.50%, -15.00% and -0.51 respectively. After using the combination of essential oils of groups C, D and E, the reaction rate of rice weevils remained above 60%. The high reaction rate indicates that the results are reliable. Figure 5 As can be seen from (1) and (2), after using the three essential oil combinations of C, D, and E, the rice weevils' tendency to feed on noodles was greatly inhibited. The three combinations of C, D, and E reduced the rice weevils' attraction rate to noodles to 22.50%, 15.00%, and 7.50%, respectively, and the relative attraction rate to -20%, -25%, and -35%, and the selection coefficient to -0.31, -0.45, and -0.7.

[0076] In the above examples, the insect control effect described in Examples 3 to 13 was evaluated by the degree of reduction of the insect attracting effect of the composite on noodles. The evaluation method of the insect attracting effect was referred to Example 2.

[0077] Table 1

[0078]

[0079] In summary, from Table 1 and Figure 3It can be seen that 5 mg / ml clove essential oil, lemon essential oil and trans-cinnamaldehyde have a good repellent effect on rice weevils, among which 5 mg / ml clove essential oil and trans-cinnamaldehyde can significantly reduce the feeding tendency of rice weevils on noodles; Figure 1 and Figure 2 It can be found that 1 mg / ml vanillin dichloromethane mixture and 5 mg / ml eicosane dichloromethane mixture can significantly reduce the rice weevils' tendency to eat noodles. Figure 4 and Figure 5 It can be seen that the combined use of 5 mg / ml cinnamaldehyde and 5 mg / ml dichloroeicosane complex has an excellent control effect on rice weevils in noodles, and is better than any repellent or attractant in controlling noodle pests. In summary, the method proposed in the present invention of using a plant-derived complex as a repellent and an attractant and using them in combination can effectively reduce the risk of pest infection in noodles.

[0080] The series of detailed descriptions listed above are only specific descriptions of feasible embodiments of the present invention, which are only used to explain the present invention and do not limit the scope of implementation of the present invention. For those skilled in the art, it is of course possible to easily make other implementation methods by replacement or change based on the technical content disclosed in this specification. Therefore, all changes and improvements made on the principles of the present invention should be included in the protection scope of the present invention.

[0081] It should be understood that although this specification is described according to various embodiments, not every embodiment contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

[0082] The series of detailed descriptions listed above are only specific descriptions of feasible embodiments of the present invention. They are not intended to limit the scope of protection of the present invention. All equivalent embodiments or changes that do not deviate from the technical spirit of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for controlling noodle pests using a plant-derived composite, characterized in that: The following steps are involved: Step S1, using plant-derived essential oil as a repellent, screening out the type and concentration range of essential oil with the best repellent effect on rice weevils, using plant-derived compounds as attractants, screening out the type and concentration range of plant-derived compounds with the best attractant effect on rice weevils, the screened repellents are clove essential oil, lemon essential oil and trans-cinnamaldehyde; the concentrations of the clove essential oil, lemon essential oil and trans-cinnamaldehyde are all 5 mg / ml; the attractant is a complex of vanillin dichloromethane, or a complex of eicosane dichloromethane; the concentration of the complex of vanillin dichloromethane is 1 mg / ml; the concentration of the complex of eicosane dichloromethane is: 1mg / ml~10mg / ml; Step S2, using the repellent screened in step S1 to control rice weevils in noodles, and obtaining the screened optimal repellent for controlling rice weevils in noodles and its concentration range, using the attractant screened in step S1 to control rice weevils in noodles, and obtaining the screened optimal attractant for controlling rice weevils in noodles and its concentration range, the optimal repellent for controlling rice weevils in noodles is clove essential oil and trans-cinnamaldehyde, and the concentrations of clove essential oil and trans-cinnamaldehyde are both 5 mg / ml; the optimal attractant for controlling rice weevils in noodles is vanillin dichloromethane complex, or eicosane dichloromethane complex; the concentration of the vanillin dichloromethane complex is 1 mg / ml; the concentration of the eicosane dichloromethane complex is 5 mg / ml; Step S3, combining the repellent and the attractant in the optimal concentration range in step S2 for controlling rice weevils in noodles to obtain the optimal combination of repellent and attractant; the optimal combination of repellent and attractant is: a combination of 5 mg / ml clove essential oil and 1 mg / ml vanillin dichloromethane complex, a combination of 5 mg / ml clove essential oil and 5 mg / ml eicosane dichloromethane complex, a combination of 5 mg / ml trans-cinnamaldehyde and 1 mg / ml vanillin dichloromethane complex, and a combination of 5 mg / ml trans-cinnamaldehyde and 5 mg / ml eicosane dichloromethane complex; Step S4, applying the optimal combination of repellent and attractant obtained in step S3 to control rice weevils in noodles, wherein the repellent is placed on the outer surface of the noodle package and the attractant is placed away from the noodles.

2. The method for controlling noodle pests using a plant-derived composite according to claim 1, characterized in that: The optimal combination of repellent and attractant obtained in step S3 is: 5 mg / ml trans-cinnamaldehyde and 5 mg / ml eicosanoid dichloromethane complex.

3. A noodle pest control agent using a plant-derived composite, characterized in that: Prepared by the method for controlling noodle pests using a plant-based composite according to claim 1 or 2.

4. An application of the method for controlling noodle pests using the plant-derived composite according to claim 1 or 2.

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