A Chinese medicine composition for treating chicken leg arthritis and its application

Abstract

The invention discloses a Chinese medicine composition for treating chicken leg arthritis and an application thereof, and relates to the technical field of Chinese veterinary medicine, wherein the Chinese medicine composition comprises the following raw materials in parts by weight: 30-45 parts of honeysuckle, 20-30 parts of forsythia, 18-25 parts of viola yedoensis, 10-17 parts of houttuynia cordata, 8-15 parts of scutellaria baicalensis, 6-10 parts of andrographis paniculata, and 3-7 parts of white moss peel. The Chinese medicine composition provided by the invention has a significant curative effect in treating viral arthritis of chicken leg.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a traditional Chinese medicine composition for treating chicken leg arthritis and application thereof. Background Art

[0002] Poultry farming is an important part of my country's animal husbandry and one of the pillar industries for the development of my country's rural economy. The National Bureau of Statistics and the Ministry of Agriculture and Rural Affairs have successively announced the situation of my country's agricultural and animal husbandry industry in 2022. In 2022, the number of poultry slaughtered nationwide was 16.14 billion, an increase of 400 million over the previous year, an increase of 2.5%, and the number of employed laborers reached more than 24 million. Therefore, poultry farming is an important industry for the development of my country's rural economy and the enrichment of farmers. Among the types of poultry raised, the number of chickens accounts for 70% of the total number of poultry raised.

[0003] In recent years, with the vigorous development of the poultry farming industry, poultry farming has developed in the direction of scale and intensiveness. Under the highly intensive farming model, poultry are prone to a variety of diseases, such as leg arthritis in chickens, which is specifically manifested as soft feet, lameness, paralysis, difficulty standing, lying down, swollen joints, swollen foot pads, claw deformation, paralysis, etc. Because the causes of occurrence are extremely complicated, such as viral arthritis caused by reovirus, mycoplasma arthritis caused by synoviae, and bacterial arthritis caused by Escherichia coli, Salmonella, and Staphylococcus. The above-mentioned pathogens that cause chicken leg arthritis are all contagious, and the occurrence of leg diseases affects the feeding of chickens, eventually leading to poor uniformity and even death of the flock, which seriously reduces the production capacity of chickens. These sick chickens cannot pass the quarantine of the quarantine department and can only be treated as defective products for harmless disposal. Therefore, the losses of the poultry farming industry caused by such diseases are as high as hundreds of millions of yuan each year.

[0004] At present, the use of antibiotics and chemical synthetic drugs, which are highly toxic, is the main means of large-scale poultry farming. However, these drugs are used in small doses and cannot play a therapeutic role. If the dose is large, it will cause secondary damage to poultry and reduce the quality of poultry products. The residual drug residues also have an adverse effect on the breeding environment. Moreover, with the abuse of these drugs, the drug resistance of viruses and bacteria has increased significantly, making it more difficult to control chicken diseases clinically. Especially for viral arthritis, Yang Jinsheng et al. studied the diagnosis and comprehensive prevention of viral arthritis in chickens, and believed that there is currently no effective antiviral drug that can completely cure the disease, and symptomatic treatment is not effective, time-consuming and labor-intensive. Once an epidemic occurs, all sick chickens should be immediately isolated, killed, incinerated, deeply buried, disinfected and other harmless treatments (Reference 1); Gu Zhaoxia studied the clinical symptoms and comprehensive prevention and control measures of viral arthritis in chickens, and believed that there is no specific drug to treat the disease, but symptomatic treatment can be performed. For chickens with mild arthritis symptoms, 2.5% pyraclostrobin injection can be used, or drugs such as virus clearing can be added to the feed for treatment. The symptoms of chronic diseased chickens are not obvious, so the disease should be prevented as the main method and treated as the auxiliary method. The main method to prevent viral arthritis in chickens is vaccination (Reference 2); Li Min et al. studied the prevalence and prevention and control of several important immunosuppressive diseases in chickens, and believed that prevention and control are mainly carried out through immunization of breeding chickens (Reference 3).

[0005] In recent years, with the development of Chinese herbal medicine in preventing and treating livestock and poultry diseases, the vast number of Chinese veterinary workers in my country have gradually deepened their research on the pharmacological activity of Chinese herbal medicines, and there have been reports on the in vitro antibacterial tests of Chinese herbal medicines on pathogens. Lu Shanshan studied arthritis caused by staphylococcal infection. After the joints were swollen and ruptured, 2% hydrogen peroxide solution was used for comprehensive cleaning, and then oxytetracycline ointment was applied. The chickens were placed in a clean and tidy environment to avoid secondary infection with other infectious diseases. Then Chinese medicine was used for prevention and treatment of the whole flock. 15g of Phellodendron, 30g of Houttuynia cordata, 25g of Angelica sinensis, 30g of Forsythia suspensa, 15g of Bletilla striata, 15g of Rhubarb, 25g of Anemarrhena asphodeloides, 20g of Rubia cordifolia, 25g of Ground Fish, 40g of Malt, and 40g of Chrysanthemum were ground into powder and added to the feed at 1g / d per chicken. It was used for one week to enhance the body's resistance and have antibacterial and anti-inflammatory effects (Reference 4). Yang Qiuhua conducted research on the diagnosis and prevention of mycoplasma infection in chickens. He took 100g of ephedra, 120g of almond, 100g of gypsum, 120g of golden buckwheat root, 100g of scutellaria, 100g of forsythia, 100g of andrographis paniculata, 100g of burdock fruit, 120g of platycodon, 100g of honeysuckle, 100g of pinellia, 150g of isatis root, and 85g of licorice. After mixing them evenly, they were crushed through a 40-mesh sieve and fed to chicks at a dose of 1.0g / d per chick and 1.5g / d per adult chicken. The medication was used continuously for 1 week (reference 5).

[0006] However, most of these prescriptions have complex ingredients and are not broad-spectrum.

[0007] References

[0008] 1. Yang Jinsheng et al. Diagnosis and comprehensive prevention of chicken viral arthritis[J]. Scientific Breeding and Farming 2018, (10), 48-49 DOI: 10.13270 / j.cnki.kxzh.2018.10.021.

[0009] 2. Gu Zhaoxia et al. Clinical symptoms and comprehensive prevention and treatment measures of chicken viral arthritis[J]. Jilin Animal Husbandry and Veterinary Medicine 2021, 42(08), 47-47+49.

[0010] 3. Li Min et al. Epidemiological status and prevention of several important immunosuppressive diseases in chickens[J]. Poultry Farming and Poultry Disease Control 2021,(10),25-32.

[0011] 4. Lu Shanshan, symptoms and prevention measures of Staphylococcus aureus infection in chickens[J]. Farmers Adviser 2023,(04),100-102.

[0012] 5. Yang Qiuhua Diagnosis and prevention of mycoplasma infection in chickens[J]. Animal Husbandry and Poultry Industry 2023, 34(03), 63-65 DOI:10.19567 / j.cnki.1008-0414.2023.03.019. Summary of the invention

[0013] In view of the problems existing in the prior art, the present invention provides a traditional Chinese medicine composition for treating chicken leg arthritis and application thereof.

[0014] The technical solution of the present invention is as follows:

[0015] A traditional Chinese medicine composition for treating arthritis in chicken legs comprises the following raw materials in parts by weight: 30-45 parts of honeysuckle, 20-30 parts of forsythia, 18-25 parts of viola yedoensis, 10-17 parts of houttuynia cordata, 8-15 parts of scutellaria baicalensis, 6-10 parts of andrographis paniculata and 3-7 parts of white moss peel.

[0016] Furthermore, the invention comprises the following raw materials in parts by weight: 38 parts of honeysuckle, 25 parts of forsythia, 21 parts of viola yedoensis, 13 parts of houttuynia cordata, 11 parts of scutellaria baicalensis, 8 parts of andrographis paniculata, and 5 parts of white moss peel.

[0017] The invention also provides a traditional Chinese medicine ointment for treating chicken leg arthritis, comprising the traditional Chinese medicine composition, refined sugar and menthol.

[0018] The invention also provides application of the above-mentioned traditional Chinese medicine ointment in preparing medicine for treating chicken leg arthritis.

[0019] Arthritis belongs to the category of "arthritis" in traditional Chinese medicine. The Yellow Emperor's Classic of Internal Medicine states that "the three qi of wind, cold and dampness come together to form arthritis". Many scholars in the past dynasties have discussed and treated arthritis from the perspective of phlegm, dampness and blood stasis. The occurrence of this disease is caused by wind, cold and dampness, and the internal factors include "heat" in addition to dampness and blood stasis. Internal heat and blood stasis easily lead to this disease. Therefore, the inventor invented this Chinese medicine composition mainly based on clearing away heat and detoxification, promoting blood circulation and dispersing stagnation, and promoting dampness and unblocking collaterals. The Chinese medicine formula of the present invention is reasonable, and the meaning of the formula is explained as follows:

[0020] Honeysuckle and Forsythia are both the main drugs, clearing away heat and detoxifying, reducing swelling and dispersing nodules. Honeysuckle is sweet and cold in nature, belongs to the lung and stomach meridians, and is dispersing in nature. It can not only relieve the heat and toxins of the internal organs, but also clear the stagnant heat in the meridians. It can clear away heat and detoxify without hurting the stomach, and its fragrance is penetrating without containing evil. Zhang Jingyue's "Compendium of Materia Medica" says: "Its nature is slightly cold and good at detoxification, so it is an important medicine for treating carbuncle, swelling, ringworm, bayberry, rheumatism and other poisons. Forsythia is bitter in taste and slightly cold in nature. It enters the heart, liver and gallbladder meridians. This product is light and floats, and its function reaches the inside and outside. "Compendium of Materia Medica" records that Forsythia has the effects of clearing heat, detoxifying, dispersing nodules and reducing swelling. Zhang Xixun's "Medical Records of Zhongzhong Canxi" says: "Forsythia has the power of rising, floating and dispersing, circulates qi and blood, and treats blood stagnation and qi accumulation in the twelve meridians. It is an important medicine for sores. It can penetrate the muscles and relieve the symptoms, clear away heat and expel wind, and is also an important medicine for treating wind-heat. The two medicines are used together to clear the heat, lift the floating and disperse the qi, and the power of clearing heat, cooling the blood and detoxifying is enhanced without causing dampness. The combination of the two medicines can also circulate qi and blood, promote qi stagnation and blood coagulation in the twelve meridians, and reduce swelling, disperse knots and relieve pain.

[0021] Viola yedoensis, Houttuynia cordata, and Herba Coptidis are the assistant drugs. Viola yedoensis is bitter and cold, and it enters the heart and liver meridians, and has the effects of clearing away heat and detoxifying, cooling blood and reducing swelling; Herba Coptidis is pungent and slightly cold, and it enters the lung meridian, and has the effects of clearing away heat and detoxifying, eliminating carbuncle and draining pus; Herba Coptidis is sweet and light, and it enters the liver, gallbladder, and small intestine meridians, and has the effects of clearing heat and dampness, detoxifying and reducing swelling. Viola yedoensis, Houttuynia cordata, and Herba Coptidis enhance the heat-clearing and detoxifying, swelling-removing and knot-dispersing power of Flos Lonicerae and Fructus Forsythiae, and they are the assistant drugs.

[0022] Andrographis paniculata is an adjuvant drug, bitter and cold in taste, and enters the lung, stomach, large intestine, and small intestine meridians. It has the effects of clearing away heat and detoxifying, cooling blood and reducing swelling. It is combined with the main and auxiliary drugs to enhance the therapeutic effect. In addition, entering the stomach, large intestine, and small intestine meridians will also have a conditioning effect on the corresponding stomach, large and small intestine digestive systems, which is helpful to improve the problem of developmental delay in sick chickens.

[0023] White moss peel is a guiding drug, bitter and cold, and enters the spleen, stomach, bladder meridian, and small intestine meridian. It has the effects of clearing heat and drying dampness, dispelling wind and detoxifying. In addition, entering the spleen and stomach meridians will also have a regulating effect on the spleen and stomach. The spleen and stomach are mutually exterior and interior, and the spleen and stomach are in the middle, which is the hub of the rise and fall of the qi mechanism. The "Yin Yang Ying Xiang Da Lun" says: The five internal organs can get stomach qi and then be unblocked. "Ben Cao Zheng Yi" says: "White fresh is a bitter and cold medicine that overcomes dampness, and can also clear the meridians and veins. "Ben Jing" treats headaches, which are caused by rheumatism and heat in the upper part; jaundice and cough are caused by dampness and heat in the middle; dampness and numbness of dead muscles, which cannot be flexed, stretched, started, stopped, or walked, and dampness and heat are numb in the joints and muscles. White fresh smell is very strong, so it can penetrate from top to bottom, clear the joints, overcome dampness and remove heat, and it is meticulous.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The present invention has a significant therapeutic effect on treating viral arthritis of chicken legs, with a cure rate of 99%.

[0026] 2. The present invention can also be used to treat other chicken leg arthritis such as synoviae mycoplasma arthritis, staphylococcal arthritis and salmonella arthritis, and has a broad spectrum.

[0027] 3. The present invention can also solve the problem of developmental delay in sick chickens, improve production efficiency and reduce breeding costs. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with embodiments so that those skilled in the art can implement the invention with reference to the description.

[0029] The present invention discloses a Chinese medicine composition for treating arthritis of chicken legs. To explain the technical content, purpose and effect of the present invention in detail, the following is an explanation in conjunction with embodiments.

[0030] Example 1 Chinese medicine composition and Chinese medicine ointment of the present invention

[0031] The Chinese medicine composition comprises the following raw materials in parts by weight: 38 parts of honeysuckle, 25 parts of forsythia, 21 parts of Viola yedoensis, 13 parts of houttuynia cordata, 11 parts of scutellaria baicalensis, 8 parts of andrographis paniculata, and 5 parts of white moss peel, and the preparation method is as follows:

[0032] (1) 7 Chinese herbal medicines including honeysuckle, forsythia, Viola yedoensis, houttuynia cordata, scutellaria baicalensis, andrographis paniculata, and Dictamni cortex are weighed according to the weight ratio of the formula, and processed into 50 mesh powder by primary wall breaking and crushing, and the powder is added with appropriate amount of boiling water, stirred into a paste, and then frozen at -25°C for 24 hours;

[0033] (2) taking out the medicinal powder frozen for 24 hours in step (1), thawing it at 25° C., and breaking the medicinal powder again by freeze-thaw principle;

[0034] (3) mixing the thawed Chinese medicine powder in step (2) with a certain amount of 95% ethanol solution, extracting at room temperature for 3 hours, filtering with suction, repeating 3 times, combining the filtrate, distilling under reduced pressure, evaporating the ethanol, and concentrating on a vacuum rotary instrument with a water bath constant temperature of 60° C. to prepare a clear paste with a relative density of 1.18-1.25. The relative density of this embodiment is 1.20;

[0035] (4) Weighing the clear paste from step (3), and weighing it with refined sugar and menthol in a weight ratio of 45:50:5;

[0036] (5) Mix refined sugar and menthol, add 50% of the weight of refined sugar in water, heat and stir until golden yellow, mix with the above-mentioned clear paste, and then concentrate to a relative density of 1.35 to prepare a Chinese medicine ointment for treating chicken leg arthritis. Refined sugar acts as an ointment-collecting agent to collect the ointment; menthol acts as a transdermal agent to help the effective ingredients of Chinese medicine penetrate the stratum corneum and skin of the chicken claw joint.

[0037] Example 2 Chinese medicine composition and Chinese medicine ointment of the present invention

[0038] The Chinese medicine composition comprises the following raw materials in parts by weight: 45 parts of honeysuckle, 20 parts of forsythia, 18 parts of viola yedoensis, 10 parts of houttuynia cordata, 8 parts of scutellaria baicalensis, 6 parts of andrographis paniculata, and 3 parts of white moss peels, and the preparation method is the same as that in Example 1.

[0039] Example 3 Chinese medicine composition and Chinese medicine ointment of the present invention

[0040] The Chinese medicine composition comprises the following raw materials in parts by weight: 30 parts of honeysuckle, 30 parts of forsythia, 25 parts of viola yedoensis, 17 parts of houttuynia cordata, 15 parts of scutellaria baicalensis, 10 parts of andrographis paniculata, and 7 parts of white moss peels. The preparation method is the same as that in Example 1.

[0041] Comparative Example 4, Control Chinese Medicine Composition 1 and Control Chinese Medicine Ointment 1

[0042] Same as Example 1, except that the weight portion of honeysuckle is 0.

[0043] Comparative Example 5, Control Chinese Medicine Composition 2 and Control Chinese Medicine Ointment 2

[0044] Same as Example 1, except that the weight portion of Fructus Forsythiae is 0.

[0045] Comparative Example 6, Comparative Chinese Medicine Composition 3 and Comparative Chinese Medicine Ointment 3

[0046] Same as Example 1, except that the weight portion of Viola yedoensis is 0.

[0047] Comparative Example 7, Comparative Chinese Medicine Composition 4 and Comparative Chinese Medicine Ointment 4

[0048] Same as Example 1, except that the weight portion of Houttuynia cordata is 0.

[0049] Comparative Example 8, Comparative Chinese Medicine Composition 5 and Comparative Chinese Medicine Ointment 5

[0050] Same as Example 1, except that the weight portion of Scutellaria baicalensis is 0.

[0051] Comparative Example 9, Comparative Chinese Medicine Composition 6 and Comparative Chinese Medicine Ointment 6

[0052] Same as Example 1, except that the weight portion of Andrographis paniculata is 0.

[0053] Comparative Example 10, Comparative Chinese Medicine Composition 7 and Comparative Chinese Medicine Ointment 7

[0054] Same as Example 1, except that the weight portion of the white moss peel is 0.

[0055] Comparison of the effects of treating toxic arthritis in chicken legs in comparative experimental cases

[0056] Construction of chicken leg arthritis type: On September 15, 2022, at the poultry research and breeding base of Jiangsu Poultry Science Research Institute in Shaobo Town, Jiangdu District, Yangzhou City, 1,100 91-day-old young Roman pink-shell laying hens were selected as experimental animals for artificial virus challenge experiments. The challenge type was reovirus that causes chicken viral arthritis.

[0057] After the infection, when all the test chickens showed symptoms of leg arthritis, the lesions were first treated with saturated sodium chloride solution (the osmotic pressure of saturated sodium chloride solution makes the pathogens in the lesion site inactive through physiological dehydration) and normal saline (further flushing the lesion site, reducing the number of pathogens in the lesion site, and restoring the osmotic pressure of the lesion site). Then the chickens were randomly divided into 11 groups, 100 in each group. Except for the different treatment methods, other conditions such as feeding and environmental conditions were the same. The grouping is as follows:

[0058] (1) The drug group of the present invention: the ointment of Example 1 was applied to the lesion once a day;

[0059] (2) Control group 1, using the control Chinese medicine ointment 1 of comparative example 4 to apply to the lesion site once a day;

[0060] (3) Control group 2, using the control Chinese medicine ointment 2 of comparative example 5 to apply to the lesion site once a day;

[0061] (4) Control group 3, using the control Chinese medicine ointment 3 of comparative example 6 to apply to the lesion site once a day;

[0062] (5) Control group 4: Apply the control Chinese medicine ointment 4 of comparative example 7 to the lesion area once a day;

[0063] (6) Control group 5: Apply the control Chinese medicine ointment 5 of comparative example 8 to the lesion area once a day;

[0064] (7) Control group 6: Apply the control Chinese medicine ointment 6 of comparative example 9 to the lesion area once a day;

[0065] (8) Control group 7: Apply the control Chinese medicine ointment 7 of comparative example 10 to the lesion area once a day;

[0066] (9) Qingjie Heji group: Qingjie Heji ([Formula] Honeysuckle, Scrophularia, Scutellaria, Rehmannia, Forsythia, Gardenia, Gentiana, Sweet Herba Lycopodiellae, Radix Isatidis, Rhizoma Anemarrhenae, Radix Ophiopogonis, Gypsum), 250 mL per bottle, mixed drink, 1 bottle with 100 kg of water, once a day;

[0067] (10) Pushapine injection group: Pushapine injection was prepared into a concentration of 2.5% and injected twice a day;

[0068] (11) Blank group, no drug treatment.

[0069] Except for the blank group (no treatment during the 5 days), the other groups were treated for 5 consecutive days. After stopping the treatment, they were observed for another 2 weeks. The survival rate, body weight, and cure rate of each group were statistically analyzed and the results are shown in Table 1.

[0070] Table 1

[0071]

[0072]

[0073] As shown in Table 1, the survival rates of the groups are similar, but the cure rates are quite different. Among them: the cure rate of the blank group is very low, indicating that if no other drug treatment is performed, only saturated sodium chloride solution and physiological saline are used to treat the lesions, and the therapeutic effect is very limited; the cure rates and curative effects of the control group 1, control group 2, control group 3, control group 4, control group 5, control group 6, control group 7, Qingjie mixture group and Pushaping injection group are equivalent; the curative effect of the drug group of the present invention is significantly better than that of the control group 1, control group 2, control group 3, control group 4, control group 5, control group 6 and control group 7, indicating that the drug composition of the present invention is reasonable, each component is indispensable, and these medicinal flavors are combined together to have an obvious synergistic effect. In addition, the drug group of the present invention can also improve the problem of developmental delay in sick chickens, improve production efficiency, and reduce breeding costs.

[0074] Efficacy Example 1 Treatment of Mycoplasma synoviae Arthritis

[0075] Construction of chicken leg arthritis type: On September 15, 2022, 300 91-day-old young Roman pink-shell laying hens were selected as experimental animals for artificial virus challenge experiments at the poultry research and breeding base of Jiangsu Poultry Science Research Institute in Shaobo Town, Jiangdu District, Yangzhou City. The virus challenge type was Mycoplasma synoviae.

[0076] After the infection, when all the experimental chickens showed symptoms of leg arthritis, the lesions were first treated with saturated sodium chloride solution and normal saline, and then the chickens were randomly divided into 3 groups, 100 in each group. Except for the different treatment methods, other conditions such as feeding and environmental conditions were the same. The grouping was as follows:

[0077] (1) The drug group of the present invention: the ointment of Example 1 was applied to the lesion once a day;

[0078] (2) Control group 01, using Xi'an Lihuang Biotechnology Co., Ltd.'s bursal quick treatment, each bag with 400 catties of drinking water, once a day;

[0079] (3) Blank group 01, no drug treatment.

[0080] Except for the blank group 01 (no treatment for 5 days), the other two groups were treated for 5 consecutive days. After stopping the treatment, they were observed for another 2 weeks. The survival rate, body weight and cure rate of each group were statistically analyzed and the results are shown in Table 2.

[0081] Table 2

[0082]

[0083]

[0084] Efficacy Example 2 Treatment of Staphylococcal Arthritis

[0085] Construction of chicken leg arthritis type: On September 15, 2022, at the poultry research and breeding base of Jiangsu Poultry Science Research Institute in Shaobo Town, Jiangdu District, Yangzhou City, 300 91-day-old young Roman pink-shell laying hens were selected as experimental animals for artificial virus challenge experiments, and the virus challenge type was Staphylococcus.

[0086] After the infection, when all the experimental chickens showed symptoms of leg arthritis, the lesions were first treated with saturated sodium chloride solution and normal saline, and then the chickens were randomly divided into 3 groups, 100 in each group. Except for the different treatment methods, other conditions such as feeding and environmental conditions were the same. The grouping was as follows:

[0087] (4) The drug group of the present invention: the ointment of Example 1 was applied to the lesion once a day;

[0088] (5) Control group 02, gentamicin was administered at 30 mg / kg drinking water, twice a day.

[0089] (6) Blank group 02, no drug treatment.

[0090] Except for the blank group 01 (no treatment for 5 days), the other two groups were treated for 5 consecutive days. After stopping the treatment, they were observed for another 2 weeks. The survival rate, body weight and cure rate of each group were statistically analyzed and the results are shown in Table 3.

[0091] Table 3

[0092]

[0093] Efficacy Example 3 Treatment of Salmonella arthritis

[0094] Construction of chicken leg arthritis type: On September 15, 2022, at the poultry research and breeding base of Jiangsu Poultry Science Research Institute in Shaobo Town, Jiangdu District, Yangzhou City, 300 91-day-old young Roman pink-shell laying hens were selected as experimental animals for artificial virus challenge experiments, and the virus challenge type was Salmonella.

[0095] After the infection, when all the experimental chickens showed symptoms of leg arthritis, the lesions were first treated with saturated sodium chloride solution and normal saline, and then the chickens were randomly divided into 3 groups, 100 in each group. Except for the different treatment methods, other conditions such as feeding and environmental conditions were the same. The grouping was as follows:

[0096] (7) The drug group of the present invention: the ointment of Example 1 was applied to the lesion once a day;

[0097] (8) Control group 03, gentamicin sulfate from Dalian Xinwei Biotechnology Co., Ltd. was administered via drinking water.

[0098] 100g gentamicin sulfate plus 1000kg water;

[0099] (9) Blank group 03, no drug treatment.

[0100] Except for the blank group 01 (no treatment during 5 days), the other two groups were treated for 5 consecutive days. After stopping the treatment, they were observed for another 2 weeks. The survival rate, body weight and cure rate of each group were statistically analyzed and the results are shown in Table 4.

[0101] Table 4

[0102]

[0103] Efficacy Example 4 Treatment of non-targeted arthritis

[0104] On February 11, 2023, at the white-feathered broiler farm in Feidong County of Anhui Hewei Group, two adjacent chicken houses, each of which can raise 50,000 AA broilers, were used as experimental chicken houses for large-scale group feeding comparison tests. One of the houses with 50,000 AA broilers was used as the experimental group. All AA broilers in this house were marketed from 1 day to 42 days of age. At any stage of feeding, after the symptoms of leg arthritis appeared, the sick chickens were picked out and raised separately in an isolated chicken house. The experimental group first treated the lesions with saturated sodium chloride solution and saline, and then applied the Chinese medicine ointment described in Example 1 to the lesions for 5 consecutive days; after the symptoms of leg arthritis occurred in the control group, the lesions were first treated with saturated sodium chloride solution and saline, and then the veterinarian in charge of the chicken farm issued a prescription for treatment, and continued to be raised until 42 days of age. The AA chickens in the experimental group and the control group were from the same batch of chicks, and the feeding conditions were the same. The results are as follows:

[0105]

[0106] Although the present invention has been described in detail above with specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made to the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A Chinese medicine composition for treating chicken leg arthritis, characterized in that: The invention is prepared from the following raw materials in parts by weight: 30-45 parts of honeysuckle, 20-30 parts of forsythia, 18-25 parts of viola yedoensis, 10-17 parts of houttuynia cordata, 8-15 parts of scutellaria baicalensis, 6-10 parts of andrographis paniculata and 3-7 parts of white moss peel.

2. A Chinese medicine composition for treating chicken leg arthritis according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 38 parts of honeysuckle, 25 parts of forsythia, 21 parts of viola yedoensis, 13 parts of houttuynia cordata, 11 parts of scutellaria baicalensis, 8 parts of andrographis paniculata and 5 parts of white moss peel.

3. A Chinese medicine ointment for treating chicken leg arthritis, characterized in that: The invention is prepared from the Chinese medicine composition according to claim 1 or 2, refined sugar and menthol.

4. Use of the Chinese medicinal ointment according to claim 3 in preparing a medicine for treating chicken leg arthritis.

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