A rice planthopper egg parasitic wasp release egg and its production method and application

Abstract

The present invention discloses a rice planthopper egg parasitic wasp release egg and a production method and application thereof, and specifically relates to the field of biological control of agricultural pests. The rice planthopper egg parasitic wasp release egg comprises an opaque shell and an egg parasitic wasp strip; wherein the shell is divided into an upper shell half and a lower shell half, and the upper shell half and the lower shell half are connected together; the egg parasitic wasp strip is located in the lower shell half and is fixed to the lower shell half via agar. The rice planthopper egg parasitic wasp release egg of the present invention can realize the batch artificial release of rice planthopper egg parasitic wasps through a light and simplified design, and a matching optimized feeding and release method, so as to give full play to the control effect of the egg parasitic wasps on rice field planthoppers and achieve the purpose of biological control of rice field planthoppers.

Description

Technical Field

[0001] The invention relates to the field of biological control of agricultural pests, and in particular to a rice planthopper egg parasitic wasp release egg and a production method and application thereof, and further to a device for batch breeding and artificial release of rice planthopper egg parasitic wasps in paddy fields and a use method thereof. Background Art

[0002] Rice planthoppers are one of the major pests of rice. They reproduce quickly and can cause disasters in a short period of time, seriously reducing rice yield and quality. At present, in addition to using chemical pesticides, the method of controlling rice planthoppers is to use the natural enemy of rice planthoppers, egg parasitic wasps, which are green and sustainable and have received widespread attention.

[0003] Egg parasitoids lay their eggs inside host eggs and feed on the nutrients inside the host eggs to grow and develop, causing the host eggs to fail to develop normally and lead to death. Among them, Mymaridae and Trichogrammatidae are the main egg parasitoids of rice planthoppers. However, in rice field habitats, due to the single crop, there are few alternative host eggs for egg parasitoids to parasitize when rice planthoppers do not occur, which is not conducive to the reproduction and growth of egg parasitoid populations. As a result, during the rampant outbreak of rice planthoppers, the natural population of rice planthopper egg parasitoids is insufficient, and the prevention and control effect is limited.

[0004] This problem can be effectively solved by artificially raising rice planthopper egg parasitic wasps and releasing them in large quantities during rice planthopper outbreaks. However, since rice planthopper egg parasitic wasps are tiny, with adult body length ranging from 0.1 to 0.4 mm, although there have been reports of large-scale artificial breeding technology, there is still a technical bottleneck in carrying large numbers of live rice planthopper egg parasitic wasps to rice fields for release. Summary of the invention

[0005] To this end, the present invention provides a rice planthopper egg parasitic wasp release egg and a production method and application thereof, so as to solve the problem that it is difficult to carry a large number of live rice planthopper egg parasitic wasps to rice fields and release them.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] According to the first aspect of the present invention, a rice planthopper egg parasitoid wasp egg placement device is provided, comprising: an opaque shell and an egg parasitoid wasp strip; wherein the shell is divided into an upper shell half and a lower shell half, and the upper shell half and the lower shell half are connected together; the egg parasitoid wasp strip is located in the lower shell half and is fixed to the lower shell half via agar.

[0008] Furthermore, the shell is made of degradable plastic material.

[0009] Furthermore, the upper half of the shell is provided with a bee exit hole with a diameter of 0.1-0.3 mm for the bees parasitic on planthopper eggs to exit.

[0010] Furthermore, a plurality of serrated protrusions are provided inside the lower half of the shell body for fixing the agar to prevent sliding.

[0011] Furthermore, the agar is prepared by dissolving food-grade agar powder in a ratio of agar powder to pure water = 2-4:100 at 80-90°C and then pouring it into the lower half of the shell of the released egg and cooling it naturally; the agar is fixed to the lower half of the released egg, and its functions are: 1) fixing the egg parasitic bee strip, 2) maintaining the humidity of the strip, and 3) positioning the center of gravity of the released egg at the bottom, so that the released egg forms a "tumbler" shape.

[0012] According to a second aspect of the present invention, a method for preparing rice planthopper egg parasitic wasp eggs comprises:

[0013] Step 1: Preparation of egg parasitic wasp strips

[0014] The rice seedlings grown for 10-20 days after sowing are covered with a sufficient number of unmated sexually mature short-winged female brown planthoppers after the upper part of the leaf stems of the plants are removed, leaving only the main stems of 3-4 cm in length;

[0015] After 48 hours, all brown planthoppers in the net cage are removed, and then a sufficient number of newly emerged rice planthopper egg parasitoids are placed in the net cage, and the female adults of the egg parasitoids use their ovipositors to lay eggs in the unfertilized eggs of brown planthoppers in the rice seedlings;

[0016] After 4-6 days, the above-ground part of the main stem of the rice seedling is cut off to obtain the bee strips with the egg parasitic wasp, which is the egg parasitic wasp bee strips;

[0017] Step 2: vertically insert the bee stick into the agar in the lower half of the egg shell; cover the upper half of the shell to obtain the rice planthopper egg parasitic wasp release eggs.

[0018] Furthermore, in the step 2, 10-20 egg parasitic wasp strips are inserted into the lower half of the egg shell, and the content of egg parasitic wasps in a single strip is not less than 100.

[0019] According to a third aspect of the present invention, there is provided a use of eggs released by a rice planthopper egg parasitoid in biological control of rice planthoppers in rice fields.

[0020] Furthermore, the method for using the rice planthopper egg parasitic wasp to place the eggs is to throw them into the rice field manually or by using a drone.

[0021] If the eggs of rice planthopper egg parasitic wasps are placed in a water-filled field, they can float vertically on the water surface, ensuring that the bee mouth faces upward; if they are placed on the ground, they will form a "tumbler" shape with the bee mouth facing upward.

[0022] The present invention has the following advantages:

[0023] The egg-releasing eggs of the rice planthopper egg parasitic wasp of the present invention can realize the batch artificial release of the rice planthopper egg parasitic wasp through a light and simplified design and a matching optimized breeding and releasing method, so as to give full play to the control effect of the egg parasitic wasp on the rice field planthopper and achieve the purpose of biological control of the rice field planthopper. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0025] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0026] Figure 1 A diagram of a device for placing rice planthopper egg parasitic wasps eggs provided in Example 1 of the present invention;

[0027] Figure 2 A process flow chart of the egg parasitic wasp strip provided in Example 1 of the present invention;

[0028] Among them, 1-honeycomb hole, 2-light-proof shell, 2-1-upper half of the shell, 2-2-lower half of the shell;

[0029] 3-rice main stem, 4-brown planthopper eggs parasitized by egg parasitoid wasps, 5-agar, 6-serrated protrusions, 7-rice seedlings, 8-scissors, 9-unmated short-winged female adults of brown planthoppers, 10-unfertilized eggs of brown planthoppers, 11-adults of egg parasitoid wasps of rice planthoppers, 12-unfertilized eggs of brown planthoppers parasitized by egg parasitoid wasps, 13-egg parasitoid wasp strips. DETAILED DESCRIPTION

[0030] The following is a description of the implementation of the present invention by specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] This embodiment provides a rice planthopper egg parasitic wasp egg placement:

[0033] The invention comprises an egg-releasing light-proof shell 2 and an egg parasitic wasp strip 13. The egg-releasing light-proof shell 2 is in the shape of an egg shell, and its size and shape are also similar to an egg, and it is made of degradable plastic. The shell is dark and light-proof (dark green, black, etc., the color is not limited), and is divided into an upper shell part 2-1 and a lower shell part 2-2. The two parts of the shell are integrated after being tightened by spiral patterns and are not easy to be separated.

[0034] The top of the upper half 2-1 of the shell is provided with several to dozens of bee holes 1, which are evenly distributed, and the diameter of each bee hole 1 is 0.1-0.3mm. The inside of the lower half 2-2 of the shell is provided with several serrated protrusions 6, which are used to fix the agar 5 to prevent sliding.

[0035] A scale line is arranged in the lower half 2-2 of the shell body, which is located at 4 / 5 of the height of the lower half 2-2 of the shell body, and agar 5 cooled and solidified in a jelly-like state is arranged to the scale line position.

[0036] The jelly-like agar is prepared by dissolving food-grade agar powder in a ratio of 2-4:100 (agar powder: purified water) at 80-90 degrees Celsius, and then pouring it into the lower half of the shell of the released egg, and cooling it naturally. In order to prevent agar 5 from growing mold and bacteria and affecting the emergence rate and quality of egg parasitic wasps, 2-5‰ potassium sorbate or methyl hydroxybenzoate is added during the heating of the agar solution.

[0037] Preparation of egg parasitic wasp strip 13:

[0038] The rice seedlings grown for 10-20 days after sowing are cut off from the upper part of the leaf stems of the plants with scissors, leaving only a 3-4 cm long main stem, and then placed in a mesh cage. A sufficient number of unmated short-winged female adults of brown planthoppers are placed in the mesh cage. The above brown planthoppers are all sexually mature adults that continue to grow for 3-5 days after emergence. They are allowed to lay eggs on the main stems of the rice seedlings. The eggs laid are all unfertilized eggs, that is, the eggs cannot develop into nymphs, but can provide the growth and development needs of the egg parasitic wasps that parasitize the eggs.

[0039] After 48 hours, all brown planthoppers in the net cage were removed, and then a sufficient number of newly emerged rice planthopper egg parasitoids were placed in the net cage. The female egg parasitoids used their ovipositors to lay eggs in the unfertilized eggs of brown planthoppers in the rice seedlings. After 4-6 days, the above-ground part of the main stem of the rice seedlings was cut off to obtain the parasitoid strips 13 with eggs.

[0040] The egg parasitoid wasp strips 13 are vertically inserted into the agar 5 in the lower half 2-2 of the released egg shell. Each released egg can be inserted with 10-20 strips, and the egg parasitoid content of a single strip is not less than 100. Therefore, each rice planthopper egg parasitoid wasp released egg can carry more than 1000 egg parasitoids.

[0041] After the inserted egg parasitic wasp bee strip 13 is tightened, the lower half 2-2 of the egg shell and the lower half 2-1 of the egg shell are formed into a whole, and the rice planthopper egg parasitic wasp is placed to lay eggs.

[0042] Example 2

[0043] This embodiment provides a method for biologically controlling rice planthoppers:

[0044] After the rice planthopper egg parasitic wasp eggs prepared in Example 1 are carried to the rice field, they can be thrown manually or dropped by drone.

[0045] If the eggs of the rice planthopper egg parasitic wasp are dropped into a paddy field, they can float upright on the water surface, so that the bee exit hole remains facing upward, which is conducive to the bees' escape; if they are dropped on the ground, they will form a "tumbler" shape with the bee exit hole facing upward.

[0046] Although the present invention has been described in detail above by general description and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made to the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A method for producing rice planthopper egg parasitic wasp eggs, characterized in that: The egg placing device for the rice planthopper egg parasitoid includes: a light-proof shell and an egg parasitoid bee strip; wherein the shell is divided into an upper shell half and a lower shell half, and the upper shell half and the lower shell half are connected together; the egg parasitoid bee strip is located in the lower shell half and is fixed to the lower shell half via agar; The production method comprises: Step 1: Preparation of egg parasitic wasp strips The rice seedlings grown for 10-20 days after sowing are covered with a sufficient number of unmated sexually mature short-winged female brown planthoppers after the upper part of the leaf stems of the plants are removed, leaving only the main stems of 3-4 cm in length; After 48 hours, all brown planthoppers in the net cage are removed, and then a sufficient number of newly emerged rice planthopper egg parasitoids are placed in the net cage, and the female egg parasitoids use their ovipositors to lay eggs in the unfertilized eggs of brown planthoppers in the rice seedlings; After 4-6 days, the above-ground part of the main stem of the rice seedling is cut off to obtain the bee strips with the egg parasitic wasp, which is the egg parasitic wasp bee strips; Step 2: vertically insert the bee stick into the agar in the lower half of the egg shell; cover the upper half of the shell to obtain the rice planthopper egg parasitic wasp release eggs.

2. The method for producing rice planthopper egg parasitic wasp eggs according to claim 1, characterized in that: The shell is made of degradable plastic material.

3. The method for preparing rice planthopper egg parasitic wasp eggs according to claim 2, characterized in that: The upper half of the shell is provided with a bee exit hole with a diameter of 0.1-0.3 mm for the bee exit of the planthopper egg parasitic bees.

4. The method for producing rice planthopper egg parasitic wasp eggs according to claim 2, characterized in that: The lower half of the shell is provided with a plurality of serrated protrusions for fixing the agar to prevent sliding.

5. The method for producing rice planthopper egg parasitic wasp eggs according to claim 3, characterized in that: The agar is prepared by dissolving edible-grade agar powder in a ratio of agar powder to pure water of 2-4:100 under the condition of 80-90° C., and then pouring the mixture into the shell of the lower half of the egg and cooling the mixture naturally. The agar is fixed to the lower half of the egg, and its functions are: 1) fixing the egg parasitic bee strip, 2) maintaining the moisture of the strip, and 3) positioning the center of gravity of the egg at the bottom, so that the egg forms a "tumbler" shape.

6. The method for producing rice planthopper egg parasitic wasp eggs according to claim 1, characterized in that: In the step 2, the lower half of the egg shell is released and 10-20 egg parasitic bee strips are inserted, and the content of egg parasitic bees in a single strip is not less than 100.

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