A pesticide composition containing flufenoxadiazam and its use

Abstract

The present invention belongs to the technical field of pesticide sterilization, and specifically discloses a pesticide composition containing flufenoxadiazam, wherein the pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is flufenoxadiazam, and the active ingredient B is any one of epoxiconazole, ipfentrifluconazole or pyraclostrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 40:1. The pesticide composition of the present invention has a good control effect on plant rust fungal diseases, can effectively delay the generation and development of pathogen resistance, is safe for crops, and is environmentally friendly.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide sterilization and discloses a pesticide composition containing flufenoxadiazam and application thereof. Background Art

[0002] Flufenoxadiazam belongs to the benzanilide fungicide class. Its chemical name is: 2′-fluoro-4-[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]benzanilide. Its CAS registration number is: 1839120-27-2. Its mechanism of action is as an inhibitor of histone deacetylation (HDAC). It has excellent control effects on diseases such as soybean rust and corn rust.

[0003] Epoxiconazole, CAS registration number: 133855-98-8, international common name: epoxiconazole, is a triazole systemic fungicide that can be absorbed by the stems and leaves of plants and conducted upward. It is an ergosterol biosynthesis inhibitor with therapeutic and protective effects. It is a low-toxic and safe fungicide that has a good preventive and control effect on diseases such as rust, damping-off and powdery mildew.

[0004] Ipfentrifluconazole is a new triazole fungicide developed by BASF. Its CAS registration number is 1417782-08-1. It is a C 14 -Demethylation inhibitors can effectively prevent and control a variety of pathogenic fungi, such as rust and diseases caused by Septoria, and are suitable for a variety of crops such as field crops, cash crops and specialty crops.

[0005] Pyraclostrobin is a succinate dehydrogenase inhibitor fungicide developed by Syngenta. This fungicide affects the respiratory chain electron transfer system of pathogens by acting on protein complex II, hindering their energy metabolism, inhibiting the growth of pathogens, and ultimately causing their death.

[0006] Rust is a plant disease caused by pathogenic bacteria of the order Pucciales in fungi. It harms the leaves, stems and fruits of plants. Pucciales pathogenic bacteria generally only cause local infection. The affected parts may produce small blisters or blister-like, cup-like, or hair-like objects of different colors due to the accumulation of spores. Some may also cause tumors, rough bark, clumps, and bent branches on branches, or cause leaf fall, scorched tips, and poor growth. When the rust disease is severe, the spores are densely packed into pieces, and the plants quickly die due to the evaporation of a large amount of water in the body. Rust disease is widely distributed and highly harmful, and is often seen in cereal crops, legumes, and fruit trees. Rust diseases of many crops are worldwide, and some are characterized by regional epidemics. Therefore, when the rust disease is severe, the crop fruit set rate decreases, seriously affecting the yield and crop quality.

[0007] After a large number of experimental studies, the applicant found that a reasonable combination of flufenoxadiazam and any one of fluphenazine, ipfentrifluconazole or pyraclostrobin has excellent control effects on a variety of Rust pathogens, especially for wheat rust, soybean rust and corn rust, with obvious synergistic effects, and is safe for target crops and environmentally friendly. Summary of the invention

[0008] In order to achieve the above-mentioned object, the present invention provides a pesticide composition containing flufenoxadiazam, and provides a use of the pesticide composition containing flufenoxadiazam for preventing and controlling Rust fungi. The pesticide composition of the present invention has a significant synergistic effect on Rust fungi, can effectively inhibit the generation and development of pathogenic bacteria resistance, and reduces the dosage of the agent.

[0009] In order to make the technical solution of the present invention clearer, the present invention adopts the following technical solution: a pesticide composition containing flufenoxadiazam, the pesticide composition comprising an active ingredient A and an active ingredient B, the active ingredient A is flufenoxadiazam, the active ingredient B is any one of flufenoxadiazam, ipfentrifluconazole or pyraclostrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 40:1;

[0010] Furthermore, the mass ratio of active ingredient A to active ingredient B in the pesticide composition is 1:25 to 20:1;

[0011] Furthermore, in the pesticide composition, the active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1;

[0012] Furthermore, in the pesticide composition, the active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:1;

[0013] Furthermore, the active ingredient B in the pesticide composition is ipfentrifluconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1;

[0014] Furthermore, in the pesticide composition, the active ingredient B is ipfentrifluconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:1;

[0015] Furthermore, in the pesticide composition, the active ingredient B is pyraclostrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 20:1;

[0016] Furthermore, in the pesticide composition, the active ingredient B is pyraclostrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 12:1;

[0017] Furthermore, the total weight of the pesticide composition is 100%wt, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 80% of the total weight of the pesticide composition;

[0018] Furthermore, the pesticide composition comprises, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0019] Furthermore, the pesticide composition can be prepared into any formulation form permitted in agriculture, and the formulation form is selected from a solid formulation or a liquid formulation;

[0020] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0021] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0022] Furthermore, the liquid preparation is selected from emulsifiable concentrates, suspensions or microemulsions, and the solid preparation is selected from water dispersible granules.

[0023] The present invention also discloses the use of the above pesticide composition for preventing and controlling Rustales diseases of crops;

[0024] Furthermore, the Rustales diseases include diseases caused by the following pathogenic bacteria: Puccinia, Gumbugella, Puccinia, Puccinia, Puccinia, Puccinia or Gatespeare;

[0025] Furthermore, the crops are cereal crops, legumes and fruit trees, and the Urticales diseases are wheat rust, corn rust, pear rust, bean rust or apple rust;

[0026] Furthermore, the Pucciales disease is soybean rust, corn rust or wheat rust;

[0027] Furthermore, the pesticide composition is applied to the pathogens and / or the medium in which they occur and / or their growth environment at an effective dose.

[0028] The beneficial effects of the present invention are as follows:

[0029] 1) The pesticide composition of the present invention rationally compounds compounds with different action mechanisms, and has significant control effect on diseases caused by Pucciales pathogens, especially wheat leaf rust, soybean rust and corn rust;

[0030] 2) The pesticide composition obtained by compounding the present invention is superior to a single agent in delaying the development of drug resistance and prolonging drug retention;

[0031] 3) When the fungicidal synergistic effect of the pesticide composition or preparation obtained by compounding the present invention is improved, the production cost and the use cost can be reduced, and the pesticide composition or preparation is safe for crops. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0033] Preparation Example:

[0034] Preparation Example 1: 20% flufenoxadiazam·epiconazole suspension (1:1)

[0035] Formula: 10% flufenoxadiazam, 10% flufenoxadiazam, 2% fatty alcohol polyoxyethylene ether, 4% alkylphenol polyoxyethylene ether phosphate, 2% polycarboxylic acid sodium salt, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 1% sodium sorbate, 0.5% silicone oil, deionized water to make up the balance;

[0036] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0037] Preparation Example 2: 24% flufenoxadiazam·epiconazole water dispersible granules (5:1)

[0038] Formula: 20% flufenoxadiazam, 4% epoxiconazole, 7% sodium lignin sulfonate, 2% pull open powder BX, 2% sodium dodecyl sulfate, 7% naphthalene sulfonate formaldehyde condensate, 8% ammonium sulfate, kaolin makes up the balance;

[0039] Preparation method: according to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% of water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and sieving; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0040] Preparation Example 3: 16% flufenoxadiazam·epiconazole emulsifiable concentrate (3:1)

[0041] Formula: 12% flufenoxadiazam, 4% flufenoxadiazam, 12% DMF, 12% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 15% propylene carbonate, balance xylene;

[0042] Preparation method: according to the formula ratio, the measured active ingredients, solvents and co-solvents are added into a mixing kettle and stirred to dissolve, then an emulsifier is added, and the remaining solvent is used to make up the balance, and the mixture is stirred evenly in a stirring kettle, and filtered to obtain the desired emulsifiable concentrate of the present invention.

[0043] Preparation Example 4: 20% flufenoxadiazam·ipfentrifluconazole suspension (3:1)

[0044] Formula: 15% flufenoxadiazam, 5% ipfentrifluconazole, 1.5% isomeric tridecyl alcohol polyoxyethylene ether, 1% succinate sulfonate, 3% styrenated phenol polyoxyethylene ether phosphate, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0045] Preparation method: Same as Preparation Example 1.

[0046] Preparation Example 5: 20% flufenoxadiazam·ipfentrifluconazole water dispersible granules (1:4)

[0047] Formula: 4% flufenoxadiazam, 16% ipfentrifluconazole, 3% naphthalenesulfonate formaldehyde condensate, 8% polycarboxylate sodium salt, 2% sodium lauryl sulfate, 7% white carbon black, kaolin makes up the balance;

[0048] Preparation method: Same as Preparation Example 2.

[0049] Preparation Example 6: 18% flufenoxadiazam·ipfentrifluconazole emulsifiable concentrate (1:2)

[0050] Formula: 6% flufenoxadiazam, 12% ipfentrifluconazole, 8% dimethyl sulfoxide, 10% styrylphenol polyoxyethylene ether, 1% calcium dodecylbenzene sulfonate, 20% propylene carbonate, and xylene to make up the balance;

[0051] Preparation method: Same as Preparation Example 3.

[0052] Preparation Example 7: 22% flufenoxadiazam·pyraclostrobin suspension (1:1)

[0053] Formula composition: 11% flufenoxadiazam, 11% pyraclostrobin, 3% naphthalene sulfonate, 1% Guerbet alcohol polyoxyethylene ether, 3% alkyl polyoxyethylene ether phosphate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 1% sodium benzoate, 5% propylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0054] Preparation method: Same as Preparation Example 1.

[0055] Preparation Example 8: 28% flufenoxadiazam·pyraclostrobin suspension (1:6)

[0056] Formula composition: 4% flufenoxadiazam, 24% pyraclostrobin, 1% sodium polycarboxylate, 2% fatty alcohol polyoxyethylene ether, 4% styrene phenol polyoxyethylene ether phosphate, 1% carboxymethyl cellulose, 1% magnesium aluminum silicate, 0.2% xanthan gum, 5% ethylene glycol, 0.1% sodium sorbate, 0.5% organosilicon defoamer, and deionized water to make up the balance;

[0057] Preparation method: Same as Preparation Example 1.

[0058] Preparation Example 9: 32% flufenoxadiazam·pyraclostrobin water dispersible granules (1:3)

[0059] Formula composition: 8% flufenoxadiazam, 24% pyraclostrobin, 10% sodium lignin sulfonate, 2% sodium lauryl sulfate, 5% alkylnaphthalene sulfonate formaldehyde condensate, 2% polyethylene glycol, 5% ammonium sulfate, and bentonite makes up the balance;

[0060] Preparation method: Same as Preparation Example 2.

[0061] Preparation Example 10: 35% flufenoxadiazam·pyraclostrobin water dispersible granules (3:4)

[0062] Formula composition: 15% flufenoxadiazam, 20% pyraclostrobin, 2% sodium dodecyl sulfate, 6% naphthalenesulfonate formaldehyde condensate, 10% tea saponin, 5% sodium chloride, 3% soluble starch, kaolin makes up the balance;

[0063] Preparation method: Same as Preparation Example 2.

[0064] Preparation Example 11: 26% flufenoxadiazam·pyraclostrobin emulsifiable concentrate (3:1)

[0065] Formula composition: 19.5% flufenoxadiazam, 6.5% pyraclostrobin, 10% EO / PO block copolymer, 10% dimethyl sulfoxide, 8% propylene glycol methyl ether, 1% calcium dodecylbenzene sulfonate, and xylene makes up the balance;

[0066] Preparation method: Same as Preparation Example 3.

[0067] Preparation Example 12: 13% flufenoxadiazam·pyraclostrobin emulsifiable concentrate (1:12)

[0068] Formula composition: 1% flufenoxadiazam, 12% pyraclostrobin, 10% dimethyl sulfoxide, 10% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 20% propylene carbonate, and trimethylbenzene makes up the balance;

[0069] Preparation method: Same as Preparation Example 3.

[0070] Indoor toxicity test

[0071] Example 1: Indoor activity test on wheat leaf rust

[0072] Test basis: NY / T 1156.15-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 15: Pot Method for Control of Wheat Leaf Rust".

[0073] Test target: wheat leaf rust (Puccinia recondita Roberge ex Desmaz.f.triticiErikss et Henn.).

[0074] Test crops: The wheat variety Tanmai 98 susceptible to leaf rust was selected for potting, 20 seeds were sown in each pot, and 10 plants were selected after emergence. When they grew to the 2-leaf 1-heart stage, they were numbered and kept for later use.

[0075] Experimental agents: 90% flufenoxadiazam technical, 97% flufenoxadiazam technical, 98% ipfentrifluconazole technical, 92% pyraclostrobin technical, all of which are provided by the Group’s R&D Center.

[0076] Drug preparation: Dissolve the test drug in a solvent and prepare it into a mother solution for later use.

[0077] Set the ratio of active ingredients in the mixture to:

[0078] flufenoxadiazam: 1:35, 1:20, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1, 20:1, 30:1;

[0079] flufenoxadiazam: ipfentrifluconazole is 1:35, 1:25, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1, 20:1, 30:1, 40:1;

[0080] flufenoxadiazam: 1:35, 1:20, 1:10, 1:4, 1:2, 1:1, 3:1, 5:1, 10:1, 25:1, 58:1;

[0081] The mother solution was diluted with an aqueous solution containing 0.1% Tween 80, and 5 concentrations were designed for each treatment.

[0082] Experimental method: Spray inoculation with spore suspension. After wheat seedlings are inoculated, they are cultured in the dark at 20℃ for 24 hours before treatment with the agent. The agent is sprayed evenly on the spare wheat seedlings by spraying and dried naturally. Then they are cultured at a temperature of 18-22℃ and a light ratio of L:D = (12:12)h. The experiment sets a treatment without the agent as a blank control. Each concentration treatment is repeated 4 times, with 1 pot per repetition and 10 plants per pot.

[0083] Data investigation: When the incidence rate of the blank control reaches more than 80%, the incidence rate of each treatment is investigated in a graded manner.

[0084] The grading method is:

[0085] Level 0: No spore pile;

[0086] Level 1: Spore piles occupy less than 5% of the entire leaf area;

[0087] Level 3: spore piles occupy 5-10% of the entire leaf area;

[0088] Level 5: The spore pile occupies 10-25% of the entire leaf area;

[0089] Level 7: Spore piles occupy 25-50% of the entire leaf area;

[0090] Level 9: The spore pile occupies more than 50% of the entire leaf area.

[0091] Data statistics and analysis:

[0092] The disease index is calculated according to formula (1)

[0093]

[0094] Where:

[0095] X—the disease index;

[0096] N i —the number of diseased leaves at each level;

[0097] i—the relative value level;

[0098] N—the total number of leaves investigated.

[0099] The control effect is calculated according to formula (2)

[0100]

[0101] Where:

[0102] P—the control effect, unit: %;

[0103] CK—the disease index of the blank control;

[0104] PT—the disease index of the medicament treatment.

[0105] Referring to the bioassay standard method NY / T 1156.6 - 2006, the synergistic effect of the mixed use of medicaments is evaluated according to the Sun Yunpei co-toxicity coefficient method (CTC), that is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect. The co-toxicity coefficient (CTC) is calculated according to formulas (3), (4), and (5).

[0106]

[0107] Where:

[0108] ATI—the measured toxicity index of the mixture;

[0109] S—the EC 50 , unit: milligram per liter (mg / L);

[0110] M—the EC 50 , unit: milligram per liter (mg / L).

[0111] TTI = TI A ×P A +TI B ×P B .......................(4)

[0112] Where:

[0113] TTI – Theoretical Toxicity Index of the mixture;

[0114] TI A ——Toxicity index of agent A;

[0115] P A ——The percentage of agent A in the mixture, in milligrams per liter (mg / L);

[0116] TI B ——Toxicity index of agent B;

[0117] P B ——The percentage of agent B in the mixture, in milligrams per liter (mg / L).

[0118]

[0119] Where:

[0120] CTC – Co-toxicity coefficient;

[0121] ATI——Actual Toxicity Index of Mixture;

[0122] TTI - Theoretical Toxicity Index of Mixture.

[0123] The DPS data processing software was used to calculate the test results and to obtain the toxicity regression equation and EC of the single agent and the mixtures with different ratios. 50 , and calculate the co-toxicity coefficient (CTC) of different ratios of the two drugs to screen out the optimal ratio of the test drugs.

[0124] Results of flufenoxadiazam and epoxiconazole test on wheat leaf rust virus:

[0125] Table 1 Combined toxicity test of flufenoxadiazam and epoxiconazole against wheat leaf rust

[0126] Test reagents and ratios <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC flufenoxadiazam(A) 0.3913 47.151 - - Epoxiconazole(B) 0.1845 100.000 - - A:B=1:35 0.1829 100.875 98.532 102.378 A:B=1:20 0.1710 107.895 97.483 110.680 A:B=1:10 0.1558 118.421 95.196 124.398 A:B=1:5 0.1433 128.751 91.192 141.187 A:B=1:3 0.1314 140.411 86.788 161.787 A:B=1:1 0.1287 143.357 73.575 194.844 A:B=3:1 0.1716 107.517 60.363 178.119 A:B=5:1 0.2086 88.447 55.959 158.057 A:B=10:1 0.2447 75.398 51.955 145.123 A:B=20:1 0.2947 62.606 49.667 126.051 A:B=35:1 0.3195 57.746 48.619 118.775

[0127] The results in Table 1 show that the mixture of flufenoxadiazam and epoxiconazole has a good fungicidal effect on wheat leaf rust. The results of the combined toxicity test of flufenoxadiazam and epoxiconazole at different ratios against wheat leaf rust showed that epoxiconazole had a high toxicity against wheat leaf rust, and EC 50 The EC value of flufenoxadiazam against wheat leaf rust is 0.1845 mg / L. 50The mass ratio of flufenoxadiazam to epoxiconazole was 1:35, 1:20 and 35:1, which showed an additive effect, and in the range of 1:10 to 20:1, they all showed a synergistic effect, among which the synergistic effect was most obvious when the mass ratio of flufenoxadiazam to epoxiconazole was 1:1, and the co-toxicity coefficient was 194.844.

[0128] Results of flufenoxadiazam and ipfentrifluconazole test on wheat leaf rust virus:

[0129] Table 2 Combined toxicity test of flufenoxadiazam and ipfentrifluconazole against wheat leaf rust

[0130]

[0131] The results in Table 2 show that the mixture of flufenoxadiazam and ipfentrifluconazole has a good fungicidal effect on wheat leaf rust.

[0132] The results of the combined toxicity test of flufenoxadiazam and ipfentrifluconazole at different ratios against wheat leaf rust showed that ipfentrifluconazole had a higher toxicity against wheat leaf rust. 50 The EC value of flufenoxadiazam for wheat leaf rust is 0.1042 mg / L. 50 The mass ratio of flufenoxadiazam to ipfentrifluconazole was 0.3913 mg / L. The mass ratio of flufenoxadiazam to ipfentrifluconazole was in the range of 1:25 to 40:1, and the synergistic effect was the most obvious when the mass ratio of flufenoxadiazam to ipfentrifluconazole was 3:1, with a co-toxicity coefficient of 197.528.

[0133] Results of flufenoxadiazam and pyraclostrobin test on wheat leaf rust virus:

[0134] Table 3 Combined toxicity test of flufenoxadiazam and pyraclostrobin against wheat leaf rust

[0135]

[0136]

[0137] The results in Table 3 show that the mixture of flufenoxadiazam and pyraclostrobin has a good control effect on wheat leaf rust. The results of the combined toxicity test of flufenoxadiazam and pyraclostrobin at different ratios against wheat leaf rust showed that pyraclostrobin has a high toxicity against wheat leaf rust, and the EC 50 The EC value of flufenoxadiazam against wheat leaf rust is 0.0858 mg / L. 50 The mass ratio of flufenoxadiazam to pyraclostrobin was 1:35, showing an additive effect, while in the range of 1:20 to 58:1, they all showed a synergistic effect, among which the synergistic effect was most obvious when the mass ratio of flufenoxadiazam to pyraclostrobin was 1:1, with a co-toxicity coefficient of 184.456.

[0138] Example 2: Indoor activity test of soybean rust

[0139] Test basis: "Standard Operating Specifications for Pesticide Biological Activity Testing Fungicide Volume SOP-SC-1120 Soybean Rust Potting Method".

[0140] Test target: soybean rust fungus (Phakopsorapachyrhizi Syd.).

[0141] Test crops: Select the rust-susceptible soybean variety Liaodou 15 and sow 2 seeds in each pot. After germination, keep only one plant. When the first pair of true leaves are fully unfolded, number them and keep them for later use.

[0142] Experimental agents: 90% flufenoxadiazam technical, 97% flufenoxadiazam technical, 98% ipfentrifluconazole technical, 92% pyraclostrobin technical, all of which are provided by the Group’s R&D Center.

[0143] Preparation of reagents: Select appropriate solvents and prepare the test reagents flufenoxadiazam and fluphenazine, ipfentrifluconazole and pyraclostrobin into mother solutions for use.

[0144] Set the ratio of active ingredients in the mixture to:

[0145] flufenoxadiazam: 1:10, 1:8, 1:4, 1:3, 5:1, 8:1, 10:1;

[0146] flufenoxadiazam: ipfentrifluconazole is 1:10, 1:8, 1:4, 1:2, 2:1, 4:1, 10:1;

[0147] flufenoxadiazam: 1:20, 1:12, 1:6, 1:3, 1:1, 3:1, 6:1, 12:1;

[0148] The mother solution was diluted with an aqueous solution containing 0.1% Tween 80, and 5 concentrations were designed for each treatment.

[0149] Test method: Spray the prepared solution evenly on the leaves until they are completely wet, and place them in the room to dry naturally after application. 24 hours after the treatment, inoculate with spore suspension, dry naturally, and place them in a dark and moisturizing culture at a temperature of 25°C and a humidity of 100% for more than 24 hours. Then place them in a culture room at about 25°C and a light ratio of L:D=(12:12)h for normal culture. The test set a treatment without the drug as a blank control. Each concentration treatment was repeated 4 times, with 10 pots per repeat.

[0150] Data investigation: When the incidence rate of the blank control reaches more than 80%, the incidence rate of each treatment is investigated in a graded manner. The grading method is:

[0151] Level 0: No spore pile;

[0152] Level 1: Spore piles occupy less than 5% of the entire leaf area;

[0153] Level 3: spore piles occupy 6-10% of the entire leaf area;

[0154] Level 5: Spore piles occupy 11-25% of the entire leaf area;

[0155] Level 7: Spore piles occupy 26-50% of the entire leaf area;

[0156] Level 9: The spore pile occupies more than 50% of the entire leaf area.

[0157] Data statistics and analysis:

[0158] The disease index is calculated according to formula (1)

[0159]

[0160] Where:

[0161] X—disease index;

[0162] N i —Number of diseased leaves at each level;

[0163] i—relative level value;

[0164] N—Total number of leaves surveyed.

[0165] The control effect is calculated according to formula (2)

[0166]

[0167] In the formula:

[0168] P——Control effect, unit: %;

[0169] CK——Disease index of blank control;

[0170] PT——Disease index of medicament treatment.

[0171] Referring to the bioassay standard method NY / T 1156.6 - 2006, the synergistic effect of the mixed medicaments was evaluated according to the Sun Yunpei co-toxicity coefficient method (CTC), that is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect. The co-toxicity coefficient (CTC) was calculated according to Formula (3), Formula (4), and Formula (5).

[0172]

[0173] In the formula:

[0174] ATI——Measured toxicity index of the mixture;

[0175] S——EC of the standard medicament 50 , unit: milligram per liter (mg / L);

[0176] M——EC of the mixture 50 , unit: milligram per liter (mg / L).

[0177] TTI = TI A ×P A +TI B ×P B ...................(4)

[0178] In the formula:

[0179] TTI——Theoretical toxicity index of the mixture;

[0180] TI A ——Toxicity index of medicament A;

[0181] P A ——Percentage content of medicament A in the mixture, unit: milligram per liter (mg / L);

[0182] TI B ——Toxicity index of medicament B;

[0183] P B ——Percentage content of medicament B in the mixture, unit: milligram per liter (mg / L).

[0184]

[0185] Where:

[0186] CTC – Co-toxicity coefficient;

[0187] ATI——Actual Toxicity Index of Mixture;

[0188] TTI - Theoretical Toxicity Index of Mixture.

[0189] The DPS data processing software was used to calculate the test results and to obtain the toxicity regression equation and EC of the single agent and the mixtures with different ratios. 50 , and calculate the co-toxicity coefficient (CTC) of different ratios of the two drugs to screen out the best ratio of the test drugs. Record the original data of all replicates of each treatment.

[0190] Results of the test on the efficacy of flufenoxadiazam and epoxiconazole against soybean rust virus:

[0191] Table 4 Combined toxicity test of flufenoxadiazam and epoxiconazole against soybean rust

[0192] Test reagents and ratios <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC flufenoxadiazam(A) 4.5627 45.657 - - Fluoxepiconazole (B) 2.0832 100.000 - - A:B=1:10 1.6308 127.741 95.060 134.380 A:B=1:8 1.5307 136.095 93.962 144.840 A:B=1:4 1.4842 140.358 89.131 157.474 A:B=1:3 1.4773 141.014 86.414 163.184 A:B=5:1 2.0023 104.040 54.714 190.152 A:B=8:1 2.2836 91.224 51.695 176.466 A:B=10:1 2.5107 82.973 50.597 163.986

[0193] The results in Table 4 show that the mixture of flufenoxadiazam and epoxiconazole has a good fungicidal effect on soybean rust. The results of the combined toxicity test of flufenoxadiazam and epoxiconazole at different ratios against soybean rust showed that epoxiconazole had a high toxicity against soybean rust, and the EC 50 The EC value of flufenoxadiazam against soybean rust is 2.0832 mg / L. 50 The mass ratio of flufenoxadiazam to epoxiconazole ranged from 1:10 to 10:1, and the synergistic effect was the most obvious when the mass ratio of flufenoxadiazam to epoxiconazole was 5:1, with a co-toxicity coefficient of 190.152.

[0194] Results of flufenoxadiazam and ipfentrifluconazole test on soybean rust virus:

[0195] Table 5 Combined toxicity test of flufenoxadiazam and ipfentrifluconazole against soybean rust

[0196] Test reagents and ratios EC50(mg / L) ATI TTI CTC flufenoxadiazam(A) 4.5627 72.933 - - ipfentrifluconazole(B) 3.3277 100.000 - - A:B=1:10 2.6655 124.843 97.539 127.993 A:B=1:8 2.2951 144.992 96.993 149.487 A:B=1:4 2.1123 157.539 94.587 166.556 A:B=1:2 1.9814 167.947 90.978 184.603 A:B=2:1 2.3027 144.513 81.955 176.332 A:B=4:1 2.8482 116.835 78.346 149.127 A:B=10:1 3.2546 102.246 75.393 135.617

[0197] The results in Table 5 show that the mixture of flufenoxadiazam and ipfentrifluconazole has a good fungicidal effect on soybean rust. The results of the combined toxicity test of flufenoxadiazam and ipfentrifluconazole at different ratios against soybean rust showed that ipfentrifluconazole had a high toxicity against soybean rust, and the EC 50 The mass ratio of flufenoxadiazam to ipfentrifluconazole was 1:10 to 10:1, and the synergistic effect was shown. The synergistic effect was most obvious when the mass ratio of flufenoxadiazam to ipfentrifluconazole was 1:2, and the co-toxicity coefficient was 184.603.

[0198] Results of the test on the activity of flufenoxadiazam and pyraclostrobin against soybean rust virus:

[0199] Table 6 Combined toxicity test of flufenoxadiazam and pyraclostrobin against soybean rust

[0200] Test reagents and ratios <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC flufenoxadiazam(A) 4.5627 2.115 - - Pyraclostrobin (B) 0.0965 100.000 - - A:B=1:20 0.0781 123.560 95.339 129.600 A:B=1:12 0.0664 145.331 92.470 157.165 A:B=1:6 0.0634 152.208 86.016 176.952 A:B=1:3 0.0749 128.838 75.529 170.582 A:B=1:1 0.1165 82.833 51.057 162.234 A:B=3:1 0.2277 42.380 26.586 159.407 A:B=6:1 0.4383 22.017 16.099 136.763 A:B=12:1 0.7770 12.420 9.645 128.772

[0201] The results in Table 6 show that the mixture of flufenoxadiazam and pyraclostrobin has a good fungicidal effect on soybean rust. The results of the combined toxicity test of flufenoxadiazam and pyraclostrobin at different ratios against soybean rust showed that pyraclostrobin had a high toxicity against soybean rust, and the EC 50 The mass ratio of flufenoxadiazam to pyraclostrobin ranged from 1:20 to 12:1, and the synergistic effect was the most obvious when the mass ratio of flufenoxadiazam to pyraclostrobin was 1:6, with a co-toxicity coefficient of 176.952.

[0202] Field efficacy test

[0203] Example 3: Field efficacy test of a mixture of flufenoxadiazam and epoxiconazole or ipfentrifluconazole for controlling wheat leaf rust

[0204] Test basis: Refer to "Guidelines for Field Efficacy Tests of Pesticides (I) Fungicides for Control of Cereal Rust (Leaf Rust, Stripe Rust, Stem Rust) (GB / T17980.23-2000)"

[0205] Experimental location: wheat fields in Nanfan Village, Shouguang City, Shandong Province.

[0206] Test target: wheat leaf rust (Puccinia recondita f.sp.tritici).

[0207] Experimental crops, varieties and growth conditions: Wheat (Tanmai 98).

[0208] Experimental setup: Each plot was randomly distributed, with protection rows around the plot. Each treatment was repeated 4 times, with a 20m 2 , randomly arrange the plots.

[0209] Application method: Use conventional spraying method to evenly spray each treatment solution on the front and back of the leaves.

[0210] Investigation and statistical methods: Investigate the disease index before applying the pesticide. Randomly select five representative sampling points in each plot 7 days after the first application and 10 days after the second application. Investigate 20 plants at each point and the top 3 leaves of each plant. Grade the plants based on the percentage of the diseased area on each leaf to the total leaf area.

[0211] Grading method:

[0212] Level 0: no lesions;

[0213] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0214] Level 3: The lesion area accounts for less than 6% to 25% of the entire leaf area;

[0215] Level 5: The lesion area accounts for less than 26% to 50% of the entire leaf area;

[0216] Level 7: The lesion area accounts for less than 51% to 75% of the entire leaf area;

[0217] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0218] Calculation method of drug efficacy:

[0219] The disease index and control efficacy were calculated using the following formula

[0220]

[0221]

[0222] Where:

[0223] CK 0 — Disease index of blank control area before drug administration;

[0224] CK 1 — Disease index of blank control area after drug application;

[0225] PT 0 —Disease index before application of pesticide in the treatment area;

[0226] PT 1 —Disease index in the drug-treated area after application of the drug.

[0227] Test results and analysis:

[0228] Table 7 Results of field trials of different pesticides for wheat leaf rust control

[0229]

[0230] As shown in the test results in Table 7, the control effects of different mixtures of flufenoxadiazam and epoxiconazole on wheat leaf rust were better than those of the control. 7 days after the first application, 24% flufenoxadiazam·epoxiconazole water dispersible granules (5:1), 20% flufenoxadiazam·epoxiconazole suspension (1:1), and 16% flufenoxadiazam·epoxiconazole emulsifiable concentrate (3:1) all showed good quick-acting properties. 10 days after the second application, the combination of 20% flufenoxadiazam·epoxiconazole suspension (1:1) had the most outstanding effect on controlling wheat leaf rust, with a control efficiency of 89.09%.

[0231] Table 8 Results of field trials of different pesticides for controlling wheat leaf rust

[0232]

[0233] Note: The protective efficacy (%) in the above table is the average value of each replicate.

[0234] As shown in the test results in Table 8, the control effects of different mixed agents of flufenoxadiazam and ipfentrifluconazole on wheat leaf rust were better than those of the control agent. 7 days after the first application and 10 days after the second application, the combination of 20% flufenoxadiazam·ipfentrifluconazole suspension (3:1) had the most outstanding effect on controlling wheat leaf rust, with control effects of 82.52% and 88.45% respectively.

[0235] Example 5: Field efficacy test of flufenoxadiazam mixed with epoxiconazole or ipfentrifluconazole for controlling soybean rust

[0236] Test basis: Refer to "Guidelines for Field Efficacy Tests of Pesticides (II) Fungicides for Control of Soybean Rust (GB / T17980.89-2004)"

[0237] Experimental location: soybean field in Gaomeng Village, Xintai City, Shandong Province. The soil layer of the experimental site is deep, and the water and fertilizer management is above average, which is in line with local scientific agricultural practices.

[0238] Test target: soybean rust (Phakopsora pachyrhizi Syd.).

[0239] Experimental crops, varieties and growth conditions: soybean (Liaodou 15).

[0240] Experimental setup: Each plot was randomly distributed, with protection rows around the plot. Each treatment was repeated 4 times, with a 20m 2 .

[0241] Application method: Use conventional spraying method, apply the first application at the early stage of the disease, and apply the next application after 7 days, for a total of 2 applications. Use a backpack electric sprayer to evenly spray each treatment agent on the front and back of the leaves and branches to make it wet but not dripping.

[0242] Investigation and statistical methods: Investigate the disease index before applying the pesticide, and conduct an investigation 10 days after the last application of the pesticide. During the investigation, samples are taken at five points on the diagonal of each plot, 20 plants are investigated at each point, and 4 leaves each on the upper, middle and lower parts of each plant are investigated. The total number of leaves and the number of diseased leaves at each level are recorded.

[0243] Grading method:

[0244] Level 0: no lesions;

[0245] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0246] Level 3: The lesion area accounts for less than 6% to 25% of the entire leaf area;

[0247] Level 5: The lesion area accounts for less than 26% to 50% of the entire leaf area;

[0248] Level 7: The lesion area accounts for less than 51% to 75% of the entire leaf area;

[0249] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0250] Calculation method of drug efficacy

[0251] The disease index and control efficacy were calculated using the following formula:

[0252]

[0253]

[0254] Where:

[0255] CK 0 — Disease index of blank control area before drug administration;

[0256] CK 1 — Disease index of blank control area after drug application;

[0257] PT 0 —Disease index before application of pesticide in the treatment area;

[0258] PT 1 —Disease index in the drug-treated area after application of the drug.

[0259] Results and analysis of the field efficacy test of 30% flufenoxadiazam·epiconazole suspension in controlling soybean rust:

[0260] Table 9 Results of field efficacy test on 30% flufenoxadiazam·epiconazole suspension for controlling soybean rust

[0261]

[0262]

[0263] Note: The protective efficacy (%) in the above table is the average value of each replicate.

[0264] As shown in the test results in Table 9, 24% flufenoxadiazam·epiconazole water dispersible granules (5:1) showed good control effect on soybean rust. 2 , 70g / hm 2 and 100g / hm 2 The average control effects of the three treatments on soybean rust were 84.76%, 87.34% and 90.05% respectively. The control effect was best when the active ingredient was 100 g / ha. The control effect of the compound preparation at high concentration was better than that at low concentration. 2 、125g / L Fluoxepoxazole suspension active ingredient dosage 125g / hm 2 The protective effects were 76.45% and 78.47% respectively.

[0265] Results and analysis of field efficacy test of 20% flufenoxadiazam·ipfentrifluconazole suspension in controlling soybean rust:

[0266] Table 10 Results of field efficacy test of 20% flufenoxadiazam·ipfentrifluconazole suspension in controlling soybean rust

[0267]

[0268] Note: The protective efficacy (%) in the above table is the average value of each replicate.

[0269] As shown in the test results in Table 10, 18% flufenoxadiazam·ipfentrifluconazole EC (1:2) showed good control effect on soybean rust. 2 、80g / hm 2 and 100g / hm 2 The average control effects of the three treatments on soybean rust were 84.54%, 88.57% and 90.85% respectively. 2 The control effect of the treatment was the best, and the control effect of the compound preparation at high concentration was better than that at low concentration. The active ingredient dosage of the control agent 30% flufenoxadiazam suspension was 250g / hm 2 25% ipfentrifluconazole suspension active ingredient dosage 150g / hm 2 The protective effects were 76.45% and 79.43% respectively.

[0270] Example 6: Field efficacy test of flufenoxadiazam and pyraclostrobin mixed to control wheat leaf rust

[0271] Test basis: Refer to GB / T 17980.23-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Fungicides for Control of Cereal Rusts (Leaf Rust, Stripe Rust, Stem Rust)"

[0272] Experimental location: wheat field in Zhenjiazhuang Village, Jimo District, Qingdao City, Shandong Province.

[0273] Test target: wheat leaf rust (Puccinia recondita Rob. ex Desm. f. sp. tritici Erikss. et Henn.).

[0274] Experimental crops and varieties: Wheat (Shannong No. 27).

[0275] Experimental setup: Each experimental plot was arranged in random blocks, with protection rows around the plots. Each treatment was repeated 4 times, with each plot 20m 2 .

[0276] Application method: Use conventional spraying method to spray each treatment agent evenly on the front and back of wheat leaves to ensure uniform spraying. The application amount of liquid is 50kg per mu.

[0277] Survey and statistical methods: The test was conducted 10 days after the last drug application. Five representative sampling points were randomly selected in each plot, 20 plants were surveyed at each point, and the top three leaves of each plant were surveyed. The lesions on each leaf were graded according to the percentage of the entire leaf area.

[0278] Grading method:

[0279] Level 0: no lesions;

[0280] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0281] Level 3: The lesion area accounts for less than 6% to 25% of the entire leaf area;

[0282] Level 5: The lesion area accounts for less than 26% to 50% of the entire leaf area;

[0283] Level 7: The lesion area accounts for less than 51% to 75% of the entire leaf area;

[0284] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0285] Calculation method of drug efficacy

[0286] The disease index and control efficacy are calculated using the following formula:

[0287]

[0288]

[0289] Test results and analysis:

[0290] Table 11 Results of field trials of different pesticides for wheat leaf rust control

[0291]

[0292] Note: The protective efficacy (%) in the above table is the average value of each replicate.

[0293] As shown in the test results in Table 11, the control effects of different mixtures of flufenoxadiazam and pyraclostrobin on wheat leaf rust were better than those of the control. Ten days after the second application, the combination of 22% flufenoxadiazam·pyraclostrobin suspension (1:1) had the most outstanding effect on controlling wheat leaf rust, with a control efficiency of 90.93%.

[0294] Example 7: Field efficacy test of flufenoxadiazam and pyraclostrobin mixed to control soybean rust

[0295] Test basis: Refer to GB / T 17980.89-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Fungicides for Control of Soybean Rust".

[0296] Experimental location: soybean fields in Dazhouzhuang Village, Yucheng, Shandong.

[0297] Test target: soybean rust (Phakopsora pachyrhizi Syd.).

[0298] Experimental crops and varieties: soybean.

[0299] Experimental setup: Each plot was arranged in random blocks, with protection rows around the plot. Each treatment was repeated 4 times, with a 20m 2 .

[0300] Application method: Use conventional spraying method to apply the pesticide in the early stage of soybean rust, and spray the pesticide evenly on the front and back of the leaves.

[0301] Survey and statistical methods: Survey was conducted 10 days after the last application of medicine. Five representative diagonal points were randomly selected for sampling in each plot, 20 plants were surveyed at each point, and 4 leaves were surveyed on the upper, middle and lower parts of each plant, and the total number of leaves and the number of diseased leaves at each level were recorded.

[0302] Grading method:

[0303] Level 0: no lesions;

[0304] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0305] Level 3: The lesion area accounts for less than 6% to 25% of the entire leaf area;

[0306] Level 5: The lesion area accounts for less than 26% to 50% of the entire leaf area;

[0307] Level 7: The lesion area accounts for less than 51% to 75% of the entire leaf area;

[0308] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0309] Calculation method of drug efficacy

[0310] The disease index and control efficacy were calculated using the following formula

[0311]

[0312]

[0313] Table 12 Results of field efficacy test of 20% flufenoxadiazam·pyraclostrobin suspension for controlling soybean rust

[0314]

[0315]

[0316] Note: The protective efficacy (%) in the above table is the average value of each replicate.

[0317] As shown in the test results in Table 12, 28% flufenoxadiazam·pyraclostrobin suspension (1:6) showed good control effect on soybean rust. 10 days after the last application, 28% flufenoxadiazam·pyraclostrobin suspension (1:6) was applied with an active ingredient of 75g / hm 2 、100g / hm 2 and 125g / hm 2 The average control effects of the three treatments on soybean rust were 82.20%, 85.09% and 88.40% respectively. The control effect of the treatment with 125 g / ha of active ingredient was the best. The control effect of the compound preparation at high concentration was better than that at low concentration. The control agent 30% flufenoxadiazam suspension was used at an active ingredient dosage of 250 g / ha 2 , 125g / L pyraclostrobin suspension active ingredient dosage 100g / hm 2 The protective effects were 72.20% and 76.90% respectively.

[0318] Example 8: Field efficacy test of flufenoxadiazam and pyraclostrobin mixed to control corn rust

[0319] Experimental location: corn field in Zhangjiazhuang Village, Laixi City, Qingdao City, Shandong Province.

[0320] Test target: Corn rust fungus (Puccinia sorghi Schw)

[0321] Test crops and varieties: Corn (Zhengdan 958)

[0322] Experimental setup: Each plot was randomly distributed, with protection rows around the plot. Each treatment was repeated 4 times, with a 20m 2 , randomly arrange the plots.

[0323] Application method: Use the Guard WS-16 manual sprayer to evenly spray the whole plant. The application time is June 15, 2020, and the weather is cloudy on the day of application.

[0324] Survey and statistical methods: Survey was conducted 10 days after the last application of medicine. Five representative points or five diagonal points were randomly selected for sampling in each plot. Twenty plants were surveyed at each point. One leaf was selected from the top, middle and bottom of each plant for investigation. The diseased spots on each leaf were graded according to the percentage of the total leaf area.

[0325] Grading method:

[0326] Level 0: no lesions;

[0327] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0328] Level 3: The lesion area accounts for less than 6% to 25% of the entire leaf area;

[0329] Level 5: The lesion area accounts for less than 26% to 50% of the entire leaf area;

[0330] Level 7: The lesion area accounts for less than 51% to 75% of the entire leaf area;

[0331] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0332] Calculation method of drug efficacy

[0333] The disease index and control efficacy were calculated using the following formula

[0334]

[0335]

[0336] Table 13 Results of field efficacy plot test of 20% flufenoxadiazam·pyraclostrobin suspension for controlling corn rust Note: The protective efficacy (%) in the above table is the average value of each replicate.

[0337] As shown in the test results in Table 13, the control effect of different mixtures of flufenoxadiazam and pyraclostrobin on corn rust was better than that of the control. 10 days after the last application, 22% flufenoxadiazam·pyraclostrobin suspension (1:1) was applied with an active ingredient of 80g / hm 2 , 120g / hm 2 and 160g / hm 2 The average control effects of the three treatments on corn rust were 87.97%, 89.30% and 91.49% respectively. The control effect of the treatment with 160 g / ha of active ingredient was the best. The control effect of the compound preparation at high concentration was better than that at low concentration. The control agent 30% flufenoxadiazam suspension active ingredient dosage was 265 g / ha 2 , 125g / L pyraclostrobin suspension active ingredient dosage 110g / hm 2 The protective effects were 79.52% and 81.80% respectively.

[0338] Through indoor toxicity determination and field tests, the pesticide composition of the present invention, which is compounded with any one of flufenoxadiazam, ipfentrifluconazole or pyraclostrobin, shows good control effect on plant rust, especially wheat leaf rust, soybean rust and corn rust. The pesticide composition or its preparation obtained by compounding of the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging the lasting effect. In addition, no compound agent was found to cause phytotoxicity to crops in the test, indicating that when the bactericidal synergistic effect of the obtained pesticide composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0339] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A use of a pesticide composition containing flufenoxadiazam for controlling Pucciales diseases, characterized in that: The pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is flufenoxadiazam, the active ingredient B is flufenoxadiazam, the mass ratio of the active ingredient A to the active ingredient B is 1:10-10:1, and the diseases are soybean rust and wheat rust.

2. The use according to claim 1, characterized in that The total weight of the pesticide composition is 100%wt, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 80% of the total weight of the pesticide composition.

3. The use according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

4. The use according to claim 1, characterized in that The pesticide composition can be prepared into any formulation form permitted in agriculture, and the formulation form is selected from a solid formulation or a liquid formulation; The liquid preparation is selected from emulsifiable concentrate, suspension or microemulsion, and the solid preparation is selected from water dispersible granules or wettable powder.

5. The use according to claim 1, characterized in that: The pesticide composition is applied to the pathogens and / or the medium in which they occur and / or their growth environment at an effective dose.

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