A treatment method of dry model root reverse situation and micro stress interaction metabolism induction of a bark wood medicinal plant
By using the reverse simulated environment cultivation method with dry bark to simulate the root environment and create micro-stress, the problem of uneven distribution of medicinal components in bark-type woody medicinal plants is solved, and the transfer of medicinal components and yield increase are realized. This provides a new method for simulated wild and simulated environment cultivation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-05-29
- Publication Date
- 2026-03-24
AI Technical Summary
Existing cultivation techniques for Chinese medicinal herbs result in substandard levels of medicinal components or low yields. In particular, the differences in medicinal components between the dried bark and root bark of bark-type woody medicinal plants lead to resource waste, and there is a lack of effective simulated habitat cultivation methods.
The reverse simulated environment cultivation method using dry root simulation is adopted. By simulating the root environment in the dry bark or branch bark of bark-type woody medicinal plants, low-frequency and high-intensity micro-stress is created to induce the transfer of medicinal components from the dry bark to the root bark. Treatments include soil wrapping, ground cover wrapping, and short-term bark peeling stimulation.
This study significantly increases the content of medicinal components in dried bark in a short period of time, simulates the effects of long-term artificial environment cultivation, improves the quality of medicinal efficacy and increases economic yield, and provides theoretical guidance for the simulated wild and artificial environment cultivation of bark-type woody medicinal plants.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural planting and production, and in particular to a treatment method for inducing metabolic interaction between the reverse simulated environment and micro-stress of bark-type woody medicinal plants through the interaction of the rootstock. Background Technology
[0002] In medicinal plants containing bark, different bark parts (branch bark, trunk bark, root bark) are naturally connected structures with similar morphology and structure but distributed in different locations. Different bark medicinal materials can only be used from different parts. For example, only the above-ground trunk bark and branch bark of Eucommia ulmoides and cinnamon can be used medicinally, while the root bark cannot. Similarly, only the underground root bark of Morus alba and Acanthopanax senticosus can be used medicinally, while the above-ground trunk bark and branch bark cannot. However, all three parts of Magnolia officinalis can be used medicinally, including the above-ground trunk bark, branch bark, and underground root bark. For the first two types of bark medicinal materials, there is often a huge waste of non-medicinal bark resources.
[0003] Eucommia ulmoides and Magnolia officinalis are important woody medicinal plants with bark in my country. The main active ingredient in Eucommia ulmoides is pinoresinol diglucoside (PDG). Only the dried bark of Eucommia ulmoides is used medicinally; the root bark is not. Tests have shown that PDG mainly accumulates in the main bark of Eucommia ulmoides, while the content in the root bark is extremely low. The main active ingredients in Magnolia officinalis are magnolol and honokiol (MN and HN). The root bark, dried bark, and branch bark of Magnolia officinalis can all be used medicinally, but the content varies in different parts. Tests have shown that the content of active ingredients in the root bark is higher than that in the dried bark. In production, people generally select Eucommia ulmoides or Magnolia officinalis plants aged 10-15 years for bark harvesting.
[0004] The active ingredients of Chinese medicinal herbs are mainly secondary metabolites. The synthesis of these secondary metabolites is largely affected by environmental stress. Artificial cultivation of Chinese medicinal herbs using high-yield field crop cultivation techniques often results in good fertilizer and water management and low levels of "adverse environmental" stress, frequently leading to reduced medicinal efficacy or even failure to meet standards. However, if wild or semi-wild cultivation is adopted, or even if "adverse environmental" conditions such as nutrient deficiency, drought, waterlogging, ice storms, and pests are intentionally introduced during cultivation, the yield per unit area will be low, failing to meet market demand and hindering profit maximization.
[0005] In recent years, with the introduction of the concept of "simulated environment cultivation" for Chinese medicinal materials, people have gradually explored different "simulated environment cultivation" measures for various Chinese medicinal materials. The aim is to change the traditional cultivation techniques through "simulated environment cultivation" and artificially create high-frequency, low-intensity "micro-stress" in order to achieve the goal of ensuring both the efficacy and quality of Chinese medicinal materials and economic yield. Yi Shanyong compared and analyzed three planting modes of Dendrobium huoshanense: facility cultivation, under-forest cultivation, and simulated environment cultivation. He found that the quality of simulated environment cultivation was superior to that of facility and under-forest cultivation, with higher yield and significant economic benefits (Yi Shanyong, Kang Chuanzhi, Wang Wei, Song Xiangwen, Xu Tao, Lu Haibo, Luo Shulan, Liu Dong, Guo Lanping, Han Bangxing. Comparison of planting modes of Dendrobium huoshanense and analysis of the advantages of simulated environment cultivation [J]. Chinese Journal of Traditional Chinese Medicine, 2021, 46(08): 1864~1868. DOI: 10.19540 / j.cnki.cjcmm.20210225.101.). Studies by Liao Xianliang, Liang Zhiyun, et al. have found that the in vitro bioactivity of n-butanol extract from Dendrobium officinale cultivated in a semi-wild environment is significantly stronger than that from Dendrobium officinale cultivated in a greenhouse (Liao Xian, Liang Zhiyun, Hu Li, et al. Comparison of in vitro bioactivity of n-butanol extract from Dendrobium officinale cultivated in greenhouse and semi-wild environments [J]. China Pharmaceutical Industry, 2022, 31(24):43-46.). Multiple studies have shown that "simulated environment cultivation" of Chinese medicinal materials can improve their quality and increase their economic yield, and is an inevitable development direction for the artificial cultivation of Chinese medicinal materials in the future.
[0006] However, most current research and invention patents on "simulated environment cultivation," such as the simulated wild cultivation method of Sanghuang (Wu Guifen, Peng Jinyun, Lu Qihuang. A simulated wild cultivation method of Sanghuang [P]. CN113016503B, 2023~04~18.), the simulated wild planting method of Scutellaria baicalensis (Chen Caixia, He Chao, Li Xian'en, Wang Wenquan. A simulated wild planting method of Scutellaria baicalensis [P]. CN112715305B, 2022~12~20), and the simulated wild cultivation technology of Dendrobium officinale under forest (Shu Jie. Application value of Dendrobium officinale and simulated wild cultivation technology under forest [J]. Modern Agricultural Science and Technology, 2021(24):61+69.), all revolve around the simulated wild environment cultivation of herbaceous medicinal plants. However, there is a lack of relevant research and inventions on bark-type woody medicinal plants, which also play an important role in Chinese medicinal materials. The exploration of "simulated environment cultivation" measures for bark-type woody medicinal plants has great economic benefits. Unlike herbaceous medicinal plants, which must undergo vegetative or reproductive growth processes to be effective, these measures can be carried out directly on the existing basis of the woody plants. Therefore, it is an important option for exploring "simulated environment cultivation" of Chinese medicinal materials. Summary of the Invention
[0007] The purpose of this invention is to investigate whether the use of reversed-environment cultivation with simulated roots in the simulated wild and artificial environment cultivation of bark-type woody medicinal plants can induce a convergence of bark content with that of root bark in the simulated parts; and to explore whether new "micro-stress" generated by the induction measures during the induction process has an interactive inducing effect on changes in the content of medicinal components in the bark, providing experimental theory for subsequent root-trunk reversed-environment cultivation of other bark-type woody medicinal plants. Furthermore, by artificially creating low-frequency, high-intensity "micro-stress," i.e., strong bark stimulation, this invention explores whether short-term strong bark stimulation can simulate and amplify the induction effect of long-term root-trunk reversed-environment cultivation measures, thereby providing guidance for a novel short-term and effective cultivation method to stimulate an increase in the content of medicinal components in the simulated wild and artificial environment cultivation of bark-type woody medicinal plants.
[0008] This invention provides a method for inducing metabolic interaction between the simulated root system and micro-stress in the dried stems of bark-type woody medicinal plants, characterized by the following steps:
[0009] 1) Select woody medicinal plants with significant differences in the content of medicinal components in the root, trunk, and bark.
[0010] For example, Eucommia ulmoides with significantly higher content of medicinal active ingredients in its bark than in its root bark, or Magnolia officinalis with significantly lower content in its bark than in its root bark; Eucommia ulmoides and Magnolia officinalis plants aged 10 to 20 years are preferred as treatment plants.
[0011] 2) Dry simulation root environment treatment
[0012] The treatment area is the bark or branch bark (main branch or lateral branch) above the ground surface of the plant's rhizome (preferably the bark of the main stem within 2m above the ground surface of Eucommia ulmoides and Magnolia officinalis). Within the treatment area, a 0.4-0.6m long piece of bark is selected at any location to simulate the root.
[0013] The method for treating the simulated dry roots is as follows: the main bark of the plant rootstock is wrapped with a material (preferably EPE pearl cotton) to shape it at a height of 0-0.5m above the ground surface, and soil (preferably 0.1m thick) is filled inside. The surrounding area is fixed with cotton rope or tape, and the bottom perimeter is filled with soil and compacted.
[0014] Alternatively, wrap the bark above the ground of the plant's rhizome and branches (preferably the bark of the main trunk within 2m above the ground of the rhizome of Eucommia ulmoides and Magnolia officinalis) with two layers of moss, and then wrap two more layers of black cloth or film (preferably PE black cloth) over the moss, with a wrapping length of 0.4 to 0.6m, and fix it around the edges with cotton rope or tape.
[0015] Alternatively, wrap the bark above the ground of the plant's rootstock and branch bark (preferably the bark of the main trunk within 2m above the ground of the rootstock of Eucommia ulmoides and Magnolia officinalis) with two layers of black cloth or film (preferably PE black cloth), with a wrapping length of 0.4 to 0.6m, and fix it with cotton rope or tape around the edges.
[0016] If necessary, select untreated plants adjacent to the treated plants as controls, with their bark and root bark serving as positive and blank controls, respectively.
[0017] Optionally, it also includes:
[0018] 3) Short-term strong stimulation of dry skin to simulate amplification effect treatment
[0019] The treatment area is the bark or branch bark (main branch or lateral branch) above the ground surface of the rhizome of the adjacent untreated plants from the same batch of plants that have undergone simulated root treatment (preferably the main bark within 2m above the ground surface of the rhizome of Eucommia ulmoides and Magnolia officinalis plants); preferably, the treatment measures include the following steps: using long-distance wrapping and peeling treatment as a short-term strong stimulation treatment of the bark.
[0020] The long-distance wrapping treatment refers to the three dry simulated root habitat treatment operations described in step 2), except that the wrapping (or burying) length of different treatments is increased (preferably, the long-distance soil burying length is 0.8 to 1.2 m, and the long-distance ground cover or black film (+ moss) wrapping length is 2 to 3 times the film simulation treatment length).
[0021] The strong stimulation measures for peeling are as follows: Peel the main bark of the eucommia and magnolia officinalis plants within 2m above the ground surface of the rhizome, corresponding to the location of the simulated root environment treatment. The peeling width is 1 / 3 to 2 / 3 of the diameter at breast height, and the length is 0.4 to 0.6m. After peeling, wrap with plastic wrap.
[0022] Preferably, in step 1), 15-20 year old Eucommia ulmoides or Magnolia officinalis are selected.
[0023] More preferably, in step 2), the soil burial treatment area is selected from the main bark within 0.5m above the ground surface of the rhizome of Eucommia ulmoides and Magnolia officinalis, and the ground cover or black film (+moss) wrapping treatment is selected from the bark or branch bark above 0.5m above the ground surface of the rhizome of Eucommia ulmoides and Magnolia officinalis. The selected bark and branch bark must be in a completely dark environment throughout the entire induction process to simulate the growth environment of the main and lateral roots.
[0024] In a specific implementation, in step 2), the content of medicinal components in the dry bark and root bark of the treated plants and the control plants is measured over a long period of time during the appropriate harvesting period (April to June each year) and under specific treatment.
[0025] More specifically, in step 2), the main active ingredients specified in the pharmacopoeia are determined. For example, the main active ingredient of Eucommia ulmoides is pinoresinol diglucoside (PDG), and the main active ingredients of Magnolia officinalis are magnolol and honokiol (MN and HN).
[0026] Preferably, in step 3), the long-distance soil burial treatment range is selected from the main trunk bark within 1m above the ground surface of the rhizomes of Eucommia ulmoides and Magnolia officinalis, and the long-distance ground cloth or black film (+moss) wrapping treatment is selected from the trunk bark or branch bark above 1m above the ground surface of the rhizomes of Eucommia ulmoides and Magnolia officinalis.
[0027] More preferably, in step 3), untreated plants adjacent to the treated plants are selected as blank controls, and corresponding dry root simulation environment treatments are selected to evaluate the short-term strong stimulation simulation amplification effect of dry bark.
[0028] This invention provides an application of the aforementioned processing method, which is used to guide the simulated wild and artificial environment cultivation of bark-type woody medicinal plants.
[0029] Specifically, it is a cultivation method used to improve the content and quality of medicinal components in bark-type woody medicinal plants.
[0030] Advantages and beneficial effects of the present invention:
[0031] The purpose of this invention is to investigate whether the use of reversed-environment cultivation with simulated roots in the simulated wild and artificial environment cultivation of bark-type woody medicinal plants can induce a convergence of bark content with that of root bark in the simulated parts; and to explore whether new "micro-stress" generated by the induction measures during the induction process has an interactive inducing effect on changes in the content of medicinal components in the bark, providing experimental theory for subsequent root-trunk reversed-environment cultivation of other bark-type woody medicinal plants. Furthermore, by artificially creating low-frequency, high-intensity "micro-stress," i.e., strong bark stimulation, this invention explores whether short-term strong bark stimulation can simulate and amplify the induction effect of long-term root-trunk reversed-environment cultivation measures, thereby providing guidance for a novel short-term and effective cultivation method to stimulate an increase in the content of medicinal components in the simulated wild and artificial environment cultivation of bark-type woody medicinal plants. Attached Figure Description
[0032] The present invention will be further described below with reference to the accompanying drawings and examples.
[0033] Figure 1 A schematic diagram of the treatment of 0-0.5m soil burial of the main stem of Eucommia ulmoides trunk;
[0034] Figure 2 A schematic diagram of the treatment of 1-1.5m trunk of Eucommia ulmoides with simulated root black film wrapping;
[0035] Figure 3 A schematic diagram of the treatment of wrapping the main stem of Magnolia officinalis with simulated root lichen (+ moss) for 0-0.5m;
[0036] Figure 4 A schematic diagram of the treatment of wrapping the main stem of Magnolia officinalis with simulated root lichen (+moss) for 1-1.5m;
[0037] Figure 5Schematic diagram of the treatment of short-term dry bark of Magnolia officinalis with strong stimulation of soil burial of 0-1m main stem;
[0038] Figure 6 A schematic diagram of the treatment of Magnolia officinalis bark with short-term strong stimulation and long-distance wrapping of the main stem (0-1.5m).
[0039] Figure 7 A schematic diagram of the treatment of Magnolia officinalis with short-term strong stimulation of peeling half of the main stem. Detailed Implementation
[0040] To facilitate understanding of the present invention, embodiments are provided below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, the embodiments are provided so that the disclosure of the present invention will be more thorough and complete.
[0041] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used in this specification is for descriptive purposes only and is not intended to be limiting of the invention.
[0042] Example 1:
[0043] In the Eucommia ulmoides and Magnolia officinalis forests at the Chang'an Base of Hunan Agricultural University in Hunan Province, multiple Eucommia ulmoides and Magnolia officinalis plants of 15 years old, with the same habitat and similar growth were selected as the same batch of treatment plants and subjected to dry simulated root treatment.
[0044] (1) In December of the previous year, the main stem bark of Eucommia ulmoides rootstock was wrapped with EPE pearl cotton material at a depth of 0-0.5m above the ground surface and shaped. The inside was filled with 0.1m of soil, and the sides were secured with cotton rope or tape. The bottom perimeter was then filled with soil and compacted (e.g., ...). Figure 1 ); Select another batch of Eucommia ulmoides plants, and wrap the main trunk bark of the Eucommia ulmoides plants 1-1.5m above the ground directly with black PE plastic film for 0.5m, and fix it with plastic rope and tape (e.g. Figure 2 );
[0045] Throughout the entire growing season (December of the previous year to December of the following year), after treatment, plant samples were collected from the following periods: treatment period (0 months), budding period (3 months), suitable harvest period (5 months), high temperature period (8 months), and cold period (12 months). The dried bark of the treated plants, the dried bark of the untreated parts of the treated plants (control group), and the dried bark of the blank plants (blank group) were harvested. The content of the active ingredients was dynamically determined by high performance liquid chromatography.
[0046] The results of the soil-wrapped dry root simulation treatment are as follows: At the germination stage, the PDG content in the dry bark of the treated plants was 119.14% of the control group and 207.16% of the blank group, respectively; at the suitable harvesting period, it was 152.51% of the control group and 327.90% of the blank group, respectively; during the high-temperature period, it was 81.56% of the control group and 95.19% of the blank group, respectively; and at 12 months after treatment, it was 144.36% of the control group and 154.67% of the blank group, respectively.
[0047] The results of the black film-wrapped simulated root treatment are as follows: At the budding stage, the PDG content in the dried bark of the treated plants was 193.35% of the control group and 117.00% of the blank group, respectively; at the suitable harvesting period, it was 335.29% of the control group and 281.27% of the blank group, respectively; during the high-temperature period, it was 176.14% of the control group and 105.10% of the blank group, respectively; and after 12 months of treatment, it was 114.38% of the control group and 111.96% of the blank group, respectively.
[0048] Because the content of medicinal components in Eucommia ulmoides is higher in the bark than in the root bark, theoretically, the result of simulating the root with Eucommia ulmoides bark should be a decrease in the content of the bark. However, the actual result is that the content of the bark increases after induction. This indicates that there is a "micro-stress" caused by the induction measures during the induction process, which has an interactive effect on the induction of the bark and ultimately manifests as an increase in the content of the bark.
[0049] (2) In August of the previous year, the following two types of dry simulated root treatments were carried out: the dry bark of Magnolia officinalis rhizomes 0-0.5m above the ground surface was wrapped with two layers of moss, with a wrapping length of 0.5m, and then two layers of PE ground cloth were added outside the moss. Finally, it was fixed with cotton rope or transparent tape (e.g. Figure 3 Wrap the dried bark of Magnolia officinalis rhizomes 1-1.5m above the ground with two layers of moss, each layer 0.5m long. Then wrap two more layers of PE ground cover over the moss, and finally secure with cotton rope or transparent tape. Figure 4 );
[0050] Throughout the entire growing season (August of the previous year to August of the following year), after treatment, plant samples were collected from the following periods: treatment period (0 months), cold period (3 months), budding period (7 months), suitable harvest period (9 months), and high-temperature period (11 months). The dried bark of treated plants, root bark of the same plant from treated plants, dried bark of adjacent untreated plants, and root bark of adjacent untreated plants were harvested. The dynamic determination of the content of the active ingredients using high-performance liquid chromatography (HPLC) yielded the following results:
[0051] The percentages of bark in the simulated roots (0-0.5m) wrapped with ground cover and moss were 6.54%, 4.46%, 4.55%, 6.24%, and 7.90%, respectively, while the percentages of bark in the same root of the treated plants were 12.37%, 8.68%, 9.55%, 13.32%, and 14.47%, respectively.
[0052] The percentages of bark in simulated rootstocks wrapped with ground cover and moss for 1–1.5 m diameter were 4.73%, 3.02%, 3.79%, 5.21%, and 6.28%, respectively, while the percentages of bark in the same rootstock of the treated plants were 12.53%, 7.43%, 8.19%, 12.77%, and 15.59%, respectively.
[0053] The percentages of dried bark at 0–0.5 m from untreated adjacent plants were 6.97%, 3.56%, 3.63%, 5.54%, and 7.01%, respectively; while the percentages at 1–1.5 m from untreated adjacent plants were 4.83%, 2.30%, 2.96%, 4.07%, and 5.64%, respectively.
[0054] The root bark content of untreated neighboring plants was 12.49%, 7.61%, 9.69%, 11.73%, and 12.83%, respectively.
[0055] Comparing the data from the two simulated root treatments of Magnolia officinalis with the untreated data, it can be seen that both simulated root treatments can induce changes in the content of medicinal components in the bark towards the simulated root bark.
[0056] The results of this embodiment show that the simulated roots of Eucommia ulmoides and Magnolia officinalis can induce changes in the content of medicinal components in the bark. Furthermore, during the induction process of Eucommia ulmoides, there is a new "micro-stress" generated by the induction measures, which has an interactive inducing effect on the changes in the content of medicinal components in the bark.
[0057] Example 2:
[0058] In the Magnolia officinalis forest at the Chang'an Base of Hunan Agricultural University in Hunan Province, several 15-year-old Magnolia officinalis plants with consistent habitat and growth were selected as the same batch of treatment plants. In April of that year, a strong stimulation treatment was carried out, involving long-distance soil burial and long-distance wrapping of the bark with ground cloth and moss: the bark of the main trunk of Eucommia ulmoides rhizome was wrapped with EPE pearl cotton material at 0-1m above the ground surface, and the soil was filled to a thickness of 0.1m inside. The sides were fixed with cotton rope or tape, and the bottom was filled with soil and compacted (e.g., ...). Figure 5 ), and wrap the dried bark of the Magnolia officinalis rhizome 0-1.5m above the ground with two layers of moss, the wrapping length being 1m, and then add two more layers of PE ground cover over the moss, finally securing it with cotton rope or transparent tape (e.g. Figure 6 ).
[0059] After treatment, the bark of treated plants was harvested at 1 month (suitable harvest period), 2 months, and 3 months, as well as the bark of untreated plants. The content of the active ingredients was dynamically determined using high-performance liquid chromatography (HPLC). The results are as follows:
[0060] The total phenolic contents of MN and HN in the 0–0.5 m diameter of the dried bark from plants subjected to long-distance soil burial with strong stimulation were 7.45%, 7.39%, and 9.01%, respectively; while the total phenolic contents of MN and HN in the 0–0.5 m diameter of the dried bark from untreated plants were 5.54%, 6.70%, and 7.01%, respectively.
[0061] The total phenolic (MN) and total phenolic (HN) content of the bark at a depth of 1–1.5 m in plants subjected to a long-distance wrapping treatment with ground cover and moss was 6.61%, 4.25%, and 3.86%, respectively; while the total phenolic (MN) and total phenolic (HN) content of the bark at a depth of 1–1.5 m in untreated plants was 4.07%, 5.05%, and 5.64%, respectively.
[0062] Comparing the total MN and HN phenol contents at 0–0.5 m of the bark of plants treated with dry simulated roots, plants treated with strong stimulation by soil burial, and untreated plants at the appropriate harvesting period (6.24%, 7.45%, and 5.54%, respectively), and the total MN and HN phenol contents at 1–1.5 m of the bark of plants treated with dry simulated roots, plants treated with strong stimulation by long-distance wrapping, and untreated plants (5.21%, 6.61%, and 4.07%, respectively), this example demonstrates that strong stimulation by soil burial and strong stimulation by long-distance wrapping can induce changes in the content of medicinal components in the bark of the simulated root bark to those in the simulated root bark after one month. That is, short-term strong stimulation of the bark can simulate and amplify the induction effect of long-term reverse simulated root cultivation measures.
[0063] Example 3:
[0064] In the Magnolia officinalis forest at the Chang'an Base of Hunan Agricultural University in Hunan Province, several 15-year-old Magnolia officinalis plants with consistent habitat and growth were selected as the same batch of treated plants. In April of that year, a strong stimulation treatment involving peeling was applied, removing the bark from the main stem 1–1.5 m above the ground surface, with the peel width being half the diameter at breast height (DBH). Figure 7 (0.5m in length), after peeling, wrap with plastic wrap;
[0065] After treatment, the dried bark of the plants at 0–0.5 m and 1–1.5 m was harvested at 1 month (suitable harvest period), 2 months, and 3 months after treatment. The content of the active ingredients was dynamically determined by high performance liquid chromatography. The results are as follows:
[0066] The total phenolic content of MN and HN in the 0-0.5m section of the bark after strong stimulation treatment (removing 1 / 2 width of bark) was 7.11%, 6.05%, and 7.61% after 1 month (suitable harvest period), 2 months, and 3 months, respectively.
[0067] The total phenolic content (MN) and HN) of the bark from 1 to 1.5 m of bark treated with strong stimulation (removing half the width of the bark) was 3.29%, 3.85%, and 4.23% after 1 month (suitable harvest period), 2 months, and 3 months, respectively.
[0068] The total phenolic contents of MN and HN in the dry bark of untreated plants at 0–0.5 m were 5.54%, 6.70%, and 7.01%, respectively, while those at 1–1.5 m were 4.07%, 5.05%, and 5.64%, respectively.
[0069] Comparing the total MN and HN phenol contents at 0–0.5 m of the dry bark of plants with simulated roots, plants with strong stimulation after peeling, and untreated plants at the appropriate harvesting period (6.24%, 7.11%, and 5.54%, respectively), and the total MN and HN phenol contents at 1–1.5 m of the dry bark of plants with simulated roots, plants with strong stimulation after peeling, and untreated plants (5.21%, 3.29%, and 4.07%, respectively), this example demonstrates that strong stimulation after peeling can induce changes in the content of medicinal components in the dry bark after one month.
[0070] The embodiments described in this invention are merely descriptions of the implementation methods of this invention and are not intended to limit the concept and scope of this invention. Without departing from the design concept of this invention, all modifications and improvements made by those skilled in the art to the technical solutions of this invention should fall within the protection scope of this invention. The technical content for which protection is sought in this invention has been fully described in the claims.
Claims
1. A method for inducing metabolic metabolism through reverse habitat simulation and micro-stress interaction in the dried root of bark-type woody medicinal plants, characterized in that, Includes the following steps: 1) Select woody medicinal plants with varying contents of medicinal components in their roots, trunks, and bark: Eucommia ulmoides with significantly higher content of medicinal active ingredients in the dry bark than in the root bark, or Magnolia officinalis with significantly lower content in the dry bark than in the root bark, and the Eucommia ulmoides and Magnolia officinalis plants aged 10 to 20 years were selected as treatment plants. 2) Dry simulation root environment processing: Shape the main stem bark 0-0.5m above the ground surface of the plant rootstock by wrapping it with a material, fill it with soil, fix it with cotton rope or tape around the perimeter, and fill and compact the bottom perimeter with soil. Alternatively, wrap the main bark within 2m above the ground with two layers of moss, and then wrap two more layers of black cloth or black film over the moss, with a wrapping length of 0.4 to 0.6m, and secure it with cotton rope or tape around the edges. Alternatively, wrap the main bark within 2m above the ground surface of the plant's rootstock with two layers of black cloth or black film, with a wrapping length of 0.4 to 0.6m, and secure it around the perimeter with cotton rope or tape. Untreated plants adjacent to the treated plants were selected as controls, with their bark and root bark serving as positive and blank controls, respectively. Also includes: 3) Short-term strong stimulation of dry skin to simulate amplification effect: The treatment area was selected from the bark of the main stem above the ground surface of the rhizome of the untreated plants from the same batch adjacent to the simulated root treatment plants. The treatment measures included the following steps: long-distance wrapping and peeling were used as short-term strong stimulation treatment of the bark. The long-distance wrapping treatment refers to the three dry simulated root environment treatment operations described in step 2), except that the wrapping length of different treatments is increased. The long-distance soil burial length is 0.8 to 1.2 m, and the long-distance black ground cover, black film, black ground cover + moss or black film + moss wrapping length is 2 to 3 times the length of the simulated treatment. The strong stimulation measures for peeling are as follows: peel off the main bark above the ground surface of the rhizome of Eucommia ulmoides and Magnolia officinalis plants, corresponding to the location of the main stem in the simulated root environment treatment. The peeling width is 1 / 3 to 2 / 3 of the diameter at breast height, and the length is 0.4 to 0.6 m. After peeling, wrap it with plastic wrap.
2. The method for inducing metabolic metabolism through reverse habitat simulation and micro-stress interaction in the dry root of a bark-type woody medicinal plant according to claim 1, characterized in that, In step 1), select Eucommia ulmoides or Magnolia officinalis plants that are 15 to 20 years old.
3. The method for inducing metabolic metabolism through reverse habitat simulation and micro-stress interaction in the stem-simulated roots of bark-type woody medicinal plants according to claim 1, characterized in that, In step 2), the packaging material is EPE pearl cotton.
4. The method for inducing metabolic metabolism through reverse habitat simulation and micro-stress interaction in the stem-simulated roots of bark-type woody medicinal plants according to claim 1, characterized in that, In step 2), the thickness of the inner filling soil is 0.1m.
5. The method for inducing metabolic metabolism through reverse habitat simulation and micro-stress interaction in the stem-simulated roots of bark-type woody medicinal plants according to claim 1, characterized in that, In step 2), the black ground cover is a PE black ground cover.
6. The method for inducing metabolic metabolism through reverse habitat simulation and micro-stress interaction in the trunk root of a bark-type woody medicinal plant according to claim 1, characterized in that, Step 3) Select untreated plants adjacent to the treated plants as blank controls, and select corresponding dry root simulation environment treatments to evaluate the short-term strong stimulation simulation amplification effect of dry bark.
7. The application of the processing method as described in claims 1 to 6, which is used to guide the simulated wild and simulated environment cultivation of bark-type woody medicinal plants.
8. The application as described in claim 7, characterized in that, Cultivation methods for improving the content and quality of medicinal components in bark-type woody medicinal plants.