A pesticide composition for preventing and controlling momordica root-knot nematodes

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for preventing and treating root-knot nematodes of Momordica grosvenori. A pesticide composition for preventing and treating root-knot nematodes of Momordica grosvenori, wherein the active ingredients are compounded with ivermectin and chlorfenapyr, iprodione or prothioconazole. When the active ingredients of the nematicidal composition of the present invention are compounded, the co-toxicity coefficient to the second-instar larvae of the southern root-knot nematode is greater than 120, showing a synergistic effect, which can improve the control effect thereof, and further provide support for the research and development of highly effective agents for preventing and treating root-knot nematodes.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling momordica root-knot nematodes. Background Art

[0002] The fruit of Momordica grosvenori has the functions of clearing away heat and detoxifying, moistening the lungs and relieving asthma, resolving phlegm and relieving cough, and refreshing and promoting the production of body fluids. It has certain effects on preventing and treating diabetes and treating coronary heart disease. For a long time, during the planting process, the roots of Momordica grosvenori are easily attacked by root-knot nematodes, which causes the growth and development of Momordica grosvenori plants and fruits to be poor, and the fruit yield drops sharply, affecting the economic benefits of planting Momordica grosvenori. Studies have reported that without the use of nematicides, the incidence of Momordica grosvenori root-knot nematodes is as high as 86% or more, and after the Momordica grosvenori plants are attacked by root-knot nematodes, soil-borne diseases such as wilt and root rot occur seriously, causing complex attacks. Affected by the attack of root-knot nematodes, the yield of Momordica grosvenori can be reduced by 60-70%, which seriously restricts the development of the Momordica grosvenori industry.

[0003] In Guangxi, the root-knot nematodes of Momordica grosvenori are mainly southern root-knot nematodes (Meloidogyne incognita) and Javan root-knot nematodes (Meloidogyne javanica), of which the southern root-knot nematodes are the dominant population. Chemical agents are an effective and convenient way to control root-knot nematodes, but due to the long-term irrational use of a single chemical agent, the resistance of pests has been rapidly accumulated, resulting in a decrease in the control effect of chemical agents. Screening for compound formulas that have a synergistic effect on root-knot nematodes can provide support for the research and development of highly effective agents for controlling root-knot nematodes.

[0004] Ivermectin, alias: Ivermectin, Ivermectin, CAS: 70288-86-7, molecular formula: C 48 H 74 O 14 , the structural formula is as follows

[0005]

[0006] The inventors found through indoor experiments that ivermectin, when combined with chlorfenapyr, iprodione or prothioconazole, has a synergistic effect on southern root-knot nematodes, which can provide support for the research and development of highly effective agents for controlling root-knot nematodes.

[0007] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0008] The object of the present invention is to provide a pesticide composition for controlling root-knot nematodes of Momordica grosvenori, so as to solve the problem that the long-term irrational use of a single chemical agent causes the rapid accumulation of pest resistance, resulting in reduced control effect of the chemical agent on the pest.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A pesticide composition for preventing and controlling root-knot nematodes of Momordica grosvenori, wherein the active ingredients are prepared by compounding ivermectin, chlorfenapyr, iprodione or prothioconazole.

[0011] Furthermore, the mass ratio of ivermectin to chlorfenapyr is 1-13:22-1.

[0012] Furthermore, the mass ratio of ivermectin to iprodione is 1:15-1.

[0013] Furthermore, the mass ratio of ivermectin to prothioconazole is 1-10:16-1.

[0014] The invention also provides application of the pesticide composition in preventing and controlling momordica root-knot nematodes.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) When the active ingredients of the nematicidal composition of the present invention are compounded, the co-toxicity coefficient to the second-instar larvae of southern root-knot nematodes is greater than 120, showing a synergistic effect, which can improve the control effect thereof, and further provide support for the research and development of highly effective agents for controlling root-knot nematodes.

[0017] (2) The nematicidal composition of the present invention has a different nematicidal action mechanism and can delay the development of drug resistance of root-knot nematodes. DETAILED DESCRIPTION

[0018] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Example :Indoor bioactivity test of root-knot nematodes

[0020] 1. Test object: Meloidogyne incognita

[0021] 2. Test reagents

[0022] 94% ivermectin technical, 97% chlorfenapyr technical, 96% iprodione technical, 96% prothioconazole technical, all of the above agents are commercially available.

[0023] The original drug was first dissolved in dimethyl sulfoxide to prepare a single-dose stock solution, and then diluted with a 0.1% Tween-80 aqueous solution. Multiple groups of proportions were set up, and each single dose and each group of mixed proportions were set to 5 gradient mass concentrations according to the equal ratio method, and each mass concentration drug solution was 50 mL for standby use.

[0024] 3. Test method: (Refer to "NT / Y 1833.1-2009 Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides Part 1 - Test for Inhibition of Plant Pathogenic Nematodes - Insect Immersion Method")

[0025] Pick the root-knot nematode eggs from the roots of Momordica grosvenori, wash them with water, place them on wet filter paper in a culture dish, and incubate them at 25°C to obtain the second-instar larvae of the same age; wash the cultured root-knot nematodes with water, filter them, centrifuge them at 1000r / min for 2 minutes, discard the supernatant, add water, centrifuge again, and finally resuspend the root-knot nematodes with water to 150 heads / mL for use. Use a pipette to draw 3mL of the drug solution into a test tube, then draw 3mL of the nematode suspension into the test tube, and mix the drug solution and the nematode suspension in equal amounts; use a pipette to transfer a certain volume of the above-mentioned mixed solution into the small holes of a 24-well biochemical test plate, cover it, and culture it at a constant temperature of 25°C for 24 hours; at the same time, set the treatment of 0.1% Tween-80 aqueous solution as a blank control.

[0026] Take 1 mL of the mixed solution from each treatment and observe the death of nematodes under a dissecting microscope. The number of nematodes observed in each repetition is no less than 100, and the total number of nematodes investigated and the number of dead nematodes are recorded. The criterion for determining the death of nematodes is: the nematodes are rigid and cannot bend or move when touched with a hairpin or bamboo needle; the mortality rate and adjusted mortality rate of each treatment are calculated based on the survey data.

[0027]

[0028] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0029]

[0030] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0031] 4. Data analysis: DPS software was used to perform regression analysis on the logarithmic values ​​of the concentration of each treatment agent and the corrected mortality probability value of each treatment, and the LC of each treatment agent was calculated. 50 , and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.

[0032] 5. Results

[0033] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC), CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-3.

[0034] Table 1 Indoor biological activity of ivermectin and chlorfenapyr against southern root-knot nematodes

[0035]

[0036]

[0037] As shown in Table 1, within the mass ratio of 1-13:22-1, the co-toxicity coefficient of ivermectin and chlorfenapyr to the second instar of southern root-knot nematode was greater than 120, showing a synergistic effect.

[0038] Table 2 Indoor biological activity of ivermectin and iprodione against southern root-knot nematodes

[0039] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Ivermectin 5.5631 100.0000 -- -- Iprodione 17.8587 31.1506 -- -- Ivermectin 1: Iprodione 15 12.8091 43.4308 35.4537 122.5001 Ivermectin 1: Iprodione 10 9.6218 57.8177 37.4097 154.5527 Ivermectin 1: Iprodione 5 8.8057 63.1761 42.6255 148.2119 Ivermectin 1: Iprodione 3 9.3113 59.7457 48.3630 123.5360 Ivermectin 1: Iprodione 1 7.0091 79.3697 65.5753 121.0359

[0040] It can be seen from Table 2 that within the mass ratio of 1:15-1, the co-toxicity coefficient of ivermectin and isoprodinil to the second instar of southern root-knot nematode was greater than 120, showing a synergistic effect.

[0041] Table 3 Indoor biological activity of ivermectin and prothioconazole against southern root-knot nematodes

[0042] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Ivermectin 5.5631 100.0000 -- -- Prothioconazole 9.0624 61.3866 -- -- Ivermectin 1: Prothioconazole 16 6.2225 89.4030 63.6580 140.4427 Ivermectin 1: Prothioconazole 12 3.8079 146.0936 64.3569 227.0055 Ivermectin 1: Prothioconazole 8 5.5491 100.2523 65.6770 152.6445 Ivermectin 1: Prothioconazole 4 6.1007 91.1879 69.1093 131.9474 Ivermectin 1: Prothioconazole 1 5.0091 111.0599 80.6933 137.6321 Ivermectin 4: Prothioconazole 1 4.1747 133.2575 92.2773 144.4098 Ivermectin 8: Prothioconazole 1 3.3767 164.7496 95.7096 172.1348 Ivermectin 10: Prothioconazole 1 1.9862 280.0876 96.4897 290.2772

[0043] As shown in Table 3, within the mass ratio of 1-10:16-1, the co-toxicity coefficient of ivermectin and prothioconazole to the second instar of southern root-knot nematode was greater than 120, showing a synergistic effect.

[0044] In summary, when the active ingredients of the nematicidal composition of the present invention are compounded, the co-toxicity coefficient to the second-instar larvae of southern root-knot nematodes is greater than 120, showing a synergistic effect, which can improve the control effect thereon, and further provide support for the research and development of highly effective agents for controlling root-knot nematodes.

[0045] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. An application of a nematicide pesticide composition composed of ivermectin and chlorfenapyr in controlling root-knot nematodes of Momordica grosvenori, characterized in that: The mass ratio of ivermectin to chlorfenapyr is 1-13:22-1.

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