A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl and its application

Abstract

The present invention discloses a fungicide composition containing fluorophenyletheramide and thiophanate-methyl and its application, wherein the active ingredients are fluorophenyletheramide and thiophanate-methyl. The mass ratio of fluorophenyletheramide and thiophanate-methyl is 15:1 to 1:15, and the rest are auxiliary ingredients allowed to be used and added in pesticides. The dosage form of the fungicide composition of the present invention is a suspension, a wettable powder, a water-dispersible granule, a granule, an aqueous emulsion, a microemulsion or an ultra-low volume liquid, wherein the dosage form is preferably a suspension. The composition has obvious synergistic effect and high prevention effect, and is mainly suitable for preventing and controlling rice sheath blight, wheat fusarium head blight and strawberry gray mold.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide compounding, and in particular to a fungicide composition containing fluorophenyletheramide and thiophanate-methyl and an application thereof. Background Art

[0002] Fluorophenyletheramide is a new type of SDHI fungicide, which has excellent prevention effect on rice sheath blight, and has high fungicidal activity against powdery mildew, gray mold, wheat scab, and potato late blight. Fluorophenyletheramide is systemic and conductive, and has the characteristics of rainwater resistance, low dosage, and low cost.

[0003] Benziothiazolinone is a thiazole heterocyclic compound, a new type of high-efficiency, low-toxic, broad-spectrum fungicide. Its mechanism of action is: it combines with anions on the surface of bacterial and fungal cell membranes or reacts with sulfhydryl groups to destroy the synthesis system of proteins and cell membranes, thereby inhibiting the reproduction of bacteria and fungi, interfering with the metabolism of pathogenic bacteria cells, causing physiological disorders and leading to the death of pathogens. This compound has a good preventive and control effect on diseases such as rot, canker, anthracnose, pear black spot, leaf spot, downy mildew, etc. on crops, and is very safe for crops.

[0004] In the actual process of agricultural production, the use of chemical agents is the most effective means to prevent and control crop diseases and insect pests, but the continuous use of a single agent and common agents leads to low sterilization efficiency and gradually enhanced drug resistance. In addition, the long-term continuous high-dose use of a single chemical fungicide is more likely to cause a series of problems such as residual agents and environmental pollution. The composite application of fluorophenyletheramide and thiophanate-methyl provided by the present invention can not only effectively prevent and control the occurrence of diseases, but also delay the generation of drug resistance, and has a long lasting effect. Summary of the invention

[0005] In view of the above-mentioned prior art, the purpose of the present invention is to provide a reasonable compound bactericidal composition, which can achieve synergistic enhancement and expand the bactericidal spectrum, and secondly can also effectively improve its rapid effect and lasting period, so that users can use it safely, efficiently and environmentally friendly and can greatly reduce the cost of medication.

[0006] The inventors conducted multiple indoor toxicity tests and field efficacy tests and found that fluphenazine and thiophanate-methyl have excellent control effects on common diseases such as rice sheath blight, wheat fusarium rust, cucumber powdery mildew and strawberry gray mold within a certain compound ratio range. This result was verified through further research and experiments, and the corresponding invention was completed.

[0007] Another object of the present invention is to provide a formulation of a fungicide composition containing fluorophenyletheramide and thiophanate-methyl. Another object is to provide the use of the above-mentioned fungicide composition containing fluorophenyletheramide and thiophanate-methyl in preventing and controlling plant diseases, especially rice sheath blight, wheat fusarium rust and strawberry gray mold.

[0008] In order to achieve the above objectives, the technical solution adopted by the present invention is:

[0009] The present invention relates to a bactericidal composition containing fluorophenyletheramide and thiophanate-methyl, wherein the active ingredients contained are fluorophenyletheramide and thiophanate-methyl, wherein the weight ratio of the fluorophenyletheramide to thiophanate-methyl is 15:1 to 1:15, and the most preferred weight ratio is 5:1.

[0010] In the composite composition of the present invention, the weight percentage of fluorophenyletheramide is 1% to 85%, the weight percentage of thiophanate-methyl is 1% to 30%, and the rest are adjuvants allowed and acceptable in pesticide science. Further, in the composition of the present invention, the weight percentage of the total weight of fluorophenyletheramide and thiophanate-methyl in the composition is 1% to 75%, preferably 1% to 65%.

[0011] The fungicidal composition of the present invention can be prepared into any application form suitable for use in agricultural production, such as suspension, wettable powder, water-dispersible granules, granules, aqueous emulsion, microemulsion or ultra-low volume liquid, among which the preferred form is suspension.

[0012] The various application formulations prepared by the fungicide composition of the present invention include, in addition to the active ingredients, various auxiliary ingredients permitted and acceptable in pesticides, including but not limited to: solvents, penetrants, dispersants, diffusing agents, disintegrants, emulsifiers, stabilizers, wetting agents, thickeners, defoaming agents, antifreeze agents, etc.

[0013] The production processes of various application dosage forms in the compound composition described in the present invention belong to the existing known technologies and will not be described in detail here.

[0014] The composite fungicidal composition of fluorophenyletheramide and thiophanate-methyl is used for preventing and controlling plant diseases, such as field crop diseases, vegetable diseases, etc., and is preferably used for preventing and controlling diseases such as rice sheath blight, wheat fusarium head blight and strawberry gray mold.

[0015] The invention discloses a bactericidal composition containing fluorophenyletheramide and thiophanate-methyl. Compared with the existing single preparation, the bactericidal effect is significantly improved, the effective period is long, and the composition is safe for crops, so the composition is worthy of being popularized and used in agricultural production.

[0016] The present invention has the following advantages:

[0017] 1. Fluorophenyletheramide in the composition of the present invention is a new type of succinate dehydrogenase inhibitor with a novel mechanism of action. It shows good control effects when combined with thiophanate-methyl for the above-mentioned diseases, and the bactericidal effect of the combination of the two is significantly higher than that of a single agent, with a co-toxicity coefficient of at least 120. Such a combination not only reduces the dosage and cost of pesticides, but also reduces pesticide residues and reduces damage to the environment.

[0018] 2. The two active ingredients in the present invention have no cross-resistance with other fungicides, and have the characteristics of low dosage, high activity, high permeability, strong systemic conductivity, resistance to rain erosion, and long lasting effect. They are environmentally friendly and safe for bees, which meets the expectation of the global market to reduce the impact of plant protection products on ecosystem safety.

[0019] 3. The composition of the present invention has a good preventive and therapeutic effect on diseases such as rice sheath blight, wheat fusarium rust and strawberry gray mold, and is safe to use without adverse effects, meeting the requirements of national green development. DETAILED DESCRIPTION

[0020] The technical scheme and technical effects of the present invention are further described below in conjunction with specific experimental examples.

[0021] The percentages described in the present invention are all weight percentages, and the embodiments of the present invention are not limited to the scope of the examples. The instruments, reagents, materials, etc. involved in the following implementation cases, unless otherwise specified, are all conventional instruments, reagents, materials, etc. already in the prior art and can be obtained through regular commercial channels. The test methods, detection methods, etc. involved in the following implementation cases, unless otherwise specified, are all conventional test methods, detection methods, etc. already in the prior art. Active ingredients are all calculated as effective ingredients. This experiment adopted a method combining indoor toxicity determination and field test. First, the appropriate ratio was screened out through indoor toxicity determination, and on this basis, field tests were carried out.

[0022] The present invention will be further described in detail below with reference to the embodiments:

[0023] 1. Preparation Examples

[0024] Preparation Example 1: 15% fluorophenyletheramide+1% thiophanate-methyl, suspension.

[0025]

[0026] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0027] Preparation Example 2: 10% fluorophenyletheramide+1% thiophanate-methyl, suspension.

[0028]

[0029]

[0030] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0031] Preparation Example 3: 15% fluorophenyletheramide+3% thiophanate-methyl, suspension.

[0032]

[0033] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0034] Preparation Example 4: 15% fluorophenyletheramide+5% thiophanate-methyl, suspension.

[0035]

[0036]

[0037] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0038] Preparation Example 5: 5% fluorophenyletheramide+5% thiophanate-methyl, suspension.

[0039]

[0040] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0041] Preparation Example 6: 5% fluorophenyletheramide+15% thiophanate-methyl, suspension.

[0042]

[0043]

[0044] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0045] Preparation Example 7: 3% fluorophenyletheramide+15% thiophanate-methyl, suspension.

[0046]

[0047] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0048] Preparation Example 8: 1% fluorophenyletheramide+10% thiophanate-methyl, suspension.

[0049]

[0050]

[0051] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0052] Preparation Example 9: 1% fluorophenyletheramide+15% thiophanate-methyl, suspension.

[0053]

[0054] The above components are mixed in proportion, ground or sheared at high speed for a certain period of time, and then filtered to obtain a suspension.

[0055] Preparation Example 10: 15% Fluorophenyletheramide, suspension

[0056]

[0057] 2. Indoor bioassay activity determination

[0058] Example 1: Indoor toxicity determination of the combination of fluphenazine ether amide and thiophanate-methyl against rice sheath blight

[0059] 1.1 Test strains

[0060] Rice sheath blight was provided by China National Rice Research Institute, Fuyang District, Hangzhou, Zhejiang Province, 2021.

[0061] 1.2 Test culture medium

[0062] Potato dextrose agar (PDA) medium was used: 200 g potato, 20 g glucose, 20 g agar, and deionized water was added to 1000 ml.

[0063] 1.3 Test agents

[0064] 98% Fluorophenyletheramide technical, 95% Thiophanate technical (Shaanxi Xida Huate Technology Industry Co., Ltd.) 98% Fluorophenyletheramide technical and 95% Thiophanate technical were prepared into mother solutions with acetone, and diluted with sterile water containing 2% Tween 80 before use.

[0065] 1.4 Indoor toxicity test of fluphenazine and thiophanate-methyl against rice sheath blight

[0066] Test method: refer to "Agricultural Industry Standard of the People's Republic of China NY / T1156.2-2006", mycelium growth rate method.

[0067] Add the prepared drugs in different proportions to the sterilized PDA medium that can be cooled to 45-50°C, shake evenly and pour into the culture dish. After cooling, make a series of poisonous culture medium plates with gradient concentrations, and set the drug-free culture medium as the control (sterile water containing 2% Tween 80 is used instead of the drug). Use a puncher to take a 6mm diameter bacterial cake from the cultured rice sheath blight pathogen under sterile conditions, and then inoculate it into the center of the cooled poisonous PDA culture medium plate with the mycelium facing down. Place one piece in each dish, repeat 3 times for each treatment, and then place it in an artificial incubator at 25°C. After 9 days of cultivation, take it out and determine the colony diameter by the cross method. Take the average of the 3 replicates of each concentration. Then calculate the EC value of different ratios of drugs against pathogens. 50 , the co-toxicity coefficient method is used to calculate the co-toxicity coefficient (CTC) of the mixture and determine the synergistic effect of the mixture. The specific calculation method is as follows:

[0068] Take a single dose in the mixture as the standard dose and calculate:

[0069] Measured toxicity index (ATI) = (standard agent LC 50 / Test drug efficacy LC 50 )×100

[0070] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture.

[0071] Co-toxicity coefficient (CTC) = [actual toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100.

[0072] According to the NY / TI1547.7-2006 standard for the classification of combined effects of pesticides, a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and 80 < co-toxicity coefficient (CTC) < 120 indicates an additive effect.

[0073] Table 1 Indoor joint toxicity test of fluphenazine amide and thiophanate-methyl against rice sheath blight

[0074]

[0075]

[0076] As shown in Table 1, the EC values ​​of fluphenazine and thiophanate-methyl against rice sheath blight 50The concentrations of fluorophenoxylate and thiophanate-methyl are 0.85 mg / L and 2.61 mg / L respectively. When the ratio of fluorophenoxylate and thiophanate-methyl is 15:1-1:15, their co-toxicity coefficient (CTC) is ≥120, which shows an excellent synergistic effect on rice sheath blight, especially when the ratio is 5:1, the synergistic effect is more significant, and the co-toxicity coefficient (CTC) is 174.11. It can be seen that the combination of fluorophenoxylate and thiophanate-methyl can effectively reduce the use of active ingredients.

[0077] Example 2: Indoor toxicity determination of the combination of fluorophenyletheramide and thiophanate-methyl against wheat scab

[0078] 2.1 Test strains

[0079] The test strain, asexual spore Fusarium graminearum Schw, was provided by the Plant Protection Pathology Laboratory, College of Agriculture, Yangtze University.

[0080] 2.2 Test culture medium

[0081] Potato dextrose agar (PDA) medium was used: 200 g potato, 20 g glucose, 20 g agar, and deionized water was added to 1000 ml.

[0082] 2.3 Test agents

[0083] 98% Fluorophenyletheramide technical, 95% Thiophanate technical (Shaanxi Xida Huate Technology Industry Co., Ltd.) 98% Fluorophenyletheramide technical and 95% Thiophanate technical were prepared into mother solutions with acetone, and diluted with sterile water containing 2% Tween 80 before use.

[0084] 2.4 Indoor toxicity test of fluphenazine and thiophanate-methyl against wheat fusarium head blight

[0085] Test method: refer to "Agricultural Industry Standard of the People's Republic of China NY / T1156.2-2006", mycelium growth rate method.

[0086] Add the prepared drugs in different ratios to the sterilized PDA medium that can be cooled to 45-50°C, shake evenly and pour into the culture dish. After cooling, make a series of poisonous culture medium plates with gradient concentrations, and set the drug-free culture medium as the control (sterile water containing 2% Tween 80 is used instead of the drug). Use a puncher to take a 6mm diameter bacterial cake from the cultured wheat fusarium pathogen under sterile conditions, and then inoculate it in the center of the cooled poisonous PDA culture medium plate with the mycelium facing down. Place one piece in each dish, repeat 3 times for each treatment, and then place it in an artificial incubator at 25°C for 12 days. Use the cross method to determine the colony diameter. Take the average of the 3 replicates of each concentration. Then calculate the EC value of different ratios of drugs against pathogens. 50, the co-toxicity coefficient method is used to calculate the co-toxicity coefficient (same as before) to determine the synergistic effect of the mixture.

[0087] Table 2 Indoor joint toxicity test of fluphenazine ether amide and thiophanate-methyl against wheat fusarium head blight

[0088] Drug name and ratio Ratio EC50(mg / L) ATI TTI CTC Fluorophenyl ether amide (A) —— 1.32 100.00 Thiophanate(B) —— 4.17 31.65 A:B 15:1 0.96 137.50 95.73 143.64 A: B 10:1 1.01 130.69 93.79 139.35 A: B 5:1 0.82 160.98 88.61 181.67 A: B 3:1 1.03 128.16 82.91 154.56 A: B 1:1 1.45 91.03 65.83 138.29 A: B 1:3 1.79 73.74 48.74 151.30 A: B 1:5 1.86 70.97 43.05 164.87 A: B 1:10 2.55 51.76 37.87 136.70 A: B 1:15 2.91 45.36 35.93 126.26

[0089] As shown in Table 2, the EC values ​​of fluphenazine and thiophanate-methyl against wheat fusarium head blight are 50 The concentrations of fluorophenoxylate and thiophanate-methyl are 1.32 mg / L and 4.17 mg / L respectively. When the ratio of fluorophenoxylate and thiophanate-methyl is 15:1-1:15, their co-toxicity coefficient (CTC) is ≥120, which shows an excellent synergistic effect on wheat fusarium rust. Especially when the ratio is 5:1, the synergistic effect is more significant, with a co-toxicity coefficient (CTC) of 181.67. It can be seen that the combination of fluorophenoxylate and thiophanate-methyl can effectively reduce the amount of active ingredients used.

[0090] Example 3: Indoor toxicity determination of the combination of fluphenazine ether amide and thiophanate-methyl against strawberry gray mold

[0091] 3.1 Test strains

[0092] Strawberry gray mold (Botrytis cinerea), the pathogen is Botrytis cinerea, which was provided by the Institute of Plant Protection, Shandong Academy of Agricultural Sciences, and then preserved and cultured by the Bioassay Laboratory of Shaanxi Xida Huate Technology Industry Co., Ltd.

[0093] 3.2 Test culture medium

[0094] Potato dextrose agar (PDA) medium was used: 200 g potato, 20 g glucose, 20 g agar, and deionized water was added to 1000 ml.

[0095] 3.3 Test agents

[0096] 98% Fluorophenyletheramide technical, 95% Thiophanate technical (Shaanxi Xida Huate Technology Industry Co., Ltd.) 98% Fluorophenyletheramide technical and 95% Thiophanate technical were prepared into mother solutions with acetone, and diluted with sterile water containing 2% Tween 80 before use.

[0097] 3.4 Indoor toxicity test of fluphenazine and thiophanate-methyl against strawberry gray mold

[0098] Test method: refer to "Agricultural Industry Standard of the People's Republic of China NY / T1156.2-2006", mycelium growth rate method.

[0099] Add the prepared drugs in different ratios to the sterilized PDA medium that can be cooled to 45-50°C, shake evenly and pour into the culture dish. After cooling, make a series of poisonous culture medium plates with gradient concentrations, and set the drug-free culture medium as the control (using sterile water containing 2% Tween 80 instead of the drug). Use a puncher to take a 6mm diameter bacterial cake from the cultured strawberry gray mold pathogen under sterile conditions, and then inoculate it into the center of the cooled poisonous PDA culture medium plate. Repeat 3 times for each treatment, and then place it in an artificial incubator at 25°C for 5 days, and use the cross method to determine the colony diameter. Take the average of the 3 repetitions of each concentration. Then calculate the EC value of the different ratios of drugs against the pathogen. 50 , the co-toxicity coefficient method is used to calculate the co-toxicity coefficient (same as before) to determine the synergistic effect of the mixture.

[0100] Table 3 Indoor joint toxicity test of fluphenazine ether amide and thiophanate-methyl against strawberry gray mold

[0101]

[0102]

[0103] As shown in Table 3, the EC values ​​of fluphenazine and thiophanate-methyl against strawberry gray mold 50 The ratios of fluphenazine and thiophanate-methyl were 4.31 mg / L and 21.62 mg / L respectively. When the ratio of fluphenazine and thiophanate-methyl was 15:1-1:15, their co-toxicity coefficients (CTC) were ≥120, which showed an excellent synergistic effect on strawberry gray mold, especially when the ratio was 5:1, the synergistic effect was more significant, with a co-toxicity coefficient (CTC) of 169.75. It can be seen that the combination of fluphenazine and thiophanate-methyl can effectively reduce the use of active ingredients.

[0104] 2. Field efficacy test

[0105] Example 1: Field efficacy test of the combination of fluphenazine ether amide and thiamethoxam against rice sheath blight

[0106] Overview of the test site: The test site was selected from the area of ​​Fuyang, Zhejiang Province, where rice sheath blight has occurred seriously in recent years. The test field area is 3600m 2 The cultivation management and fertilization levels were moderate and the relative conditions were consistent. The rice variety tested was Xiushui 121.

[0107] Experimental treatment: The experimental agent was the (15+3)% fluorophenyletheramide·thiophanate-methyl suspension of Preparation Example 3, and the control agent was 5% thiophanate-methyl suspension (commercially available, Shaanxi Xida Huate Technology Industry Co., Ltd.), 15% fluorophenyletheramide suspension (laboratory homemade sample, Preparation Example 10), and the blank control was sprayed with an equal amount of clean water. The experiment adopted a randomized block design, with a total of 4 treatments, staggered. Each treatment was repeated 3 times, with a total of 12 plots, each plot was 20m long, 15m wide, and an area of ​​300m 2 , a 0.3m walkway was set between the small plots, a 0.5m walkway was set between the repeated plots, and protective rows were set up around the plots. The test was applied twice, with a backpack sprayer Singapore Linong HD-400 for stem and leaf spraying on July 15 and 22, 2022, with a spray volume of 225kg / hm 2 .

[0108] Survey method: Before the first application of pesticides (July 14), the incidence of rice sheath blight in each treatment area was investigated, and the disease index before application was calculated. 15 days after the second application of pesticides (August 6), the incidence of rice sheath blight in each treatment area was investigated, the disease index after application was calculated, and the control effect of each agent on rice sheath blight was calculated. The survey adopted the diagonal 5-point sampling method. Each point fixedly investigated 5 connected holes, a total of 25 holes, recorded the total number of investigated plants and the number of diseased plants, and graded the disease according to the degree of damage to the rice leaf sheaths and leaves. The survey was repeated 3 times for each treatment. The grading standards for rice sheath blight are as follows (in leaf position units):

[0109] Level 0: The whole plant is disease-free;

[0110] Level 1: The disease occurs on leaf sheaths or leaves below the 4th leaf blade;

[0111] Level 3: The disease occurs on leaf sheaths or leaves below the third leaf blade;

[0112] Level 5: The disease occurs on the leaf sheaths or leaves below the second leaf.

[0113] Level 7: The sword leaf or leaves are diseased;

[0114] Level 9: The whole plant becomes sick and dies.

[0115] Efficacy calculation method: Calculate according to the national standard "Guidelines for Field Efficacy Tests of Pesticides (I)"; calculate the disease index and prevention and control effect.

[0116] Disease index (%) = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants surveyed × highest level value)

[0117] Control effect (%) = (CK1-P1) / CK1×100

[0118] If rice sheath blight occurs before application, calculate according to the following formula:

[0119] Control effect (%) = [1-(disease index of control area before medication × disease index of control area after medication) / (disease index of control area after medication × disease index of control area before medication)] × 100

[0120] P1: disease index of the control area;

[0121] CK1: disease index of control area;

[0122] The results are shown in Table 4:

[0123] Table 4 Experimental results of each treatment agent for controlling rice sheath blight

[0124]

[0125] As can be seen from Table 4, the (15+3)% fluorophenyletheramide·thiophanate-methyl suspension has a good control effect on rice sheath blight, reaching 85.44%, and has no adverse effects on rice growth and filling, so it can be promoted and used in production.

[0126] Example 2: Field efficacy test of the combination of fluphenazine ether amide and thiamethoxam against wheat scab

[0127] Overview of the test site: The test site was selected from Suining, Jiangsu Province. It is a direct-seeding wheat field. The wheat variety for the test is "Yangmai 20", which is a wheat scab susceptible variety. The growth and planting density of wheat in the test field are uniform. The area of ​​the test field is 3500m 2 , cultivation, water and fertilizer management, weed control, etc. are consistent with local wheat production.

[0128] Experimental treatment: The experimental agent was the (15+3)% fluorophenyletheramide·thiophanate-methyl suspension of Preparation Example 3, and the control agent was 5% thiophanate-methyl suspension (commercially available, Shaanxi Xida Huate Technology Industry Co., Ltd.), 15% fluorophenyletheramide suspension (laboratory self-made sample, Preparation Example 10), and the blank control was sprayed with an equal amount of clean water. The experiment adopted a randomized block design, with a total of 4 treatments, staggered, and each treatment was repeated 4 times, with a total of 16 plots, each plot area of ​​44.5m 2 Conventional cultivation management, no fungicide spraying during the entire growth period of wheat. The pesticide was applied twice, the first time was applied at the early stage of wheat flowering (April 15, 2022), and the second time was applied at the middle and late stage of wheat flowering (April 25, 2022). Electric sprayers were used for both applications with 225kg / hm2 of water. 2 , the liquid should be sprayed mainly on the wheat ears on a sunny day.

[0129] Survey method: 15 days after the second application of pesticides, also during the wheat filling to milk stage, each plot was sampled in parallel and jumped in the middle position to survey 500 ears, investigate the number of diseased ears at each level, calculate the diseased ear rate, diseased ear control effect, disease index and control effect. The classification standards for wheat fusarium head blight are as follows:

[0130] Level 0: no lesions;

[0131] Level 1: The diseased ear area accounts for less than 1 / 4 of the total ear area;

[0132] Level 2: The diseased ear area accounts for 1 / 4 to 1 / 2 of the whole ear area;

[0133] Level 3: The diseased ear area accounts for 1 / 2 to 3 / 4 of the whole ear area;

[0134] Level 4: The diseased ear area accounts for more than 3 / 4 of the total ear area.

[0135] Then, the disease index and control effect of each treatment were calculated as follows:

[0136] Disease index = 100 × ∑ (number of diseased ears × disease level) / (total number of investigated ears × 4)

[0137] Diseased ear prevention effect (%) = (number of diseased ears in blank control area - number of diseased ears in control area) / number of diseased ears in blank control area × 100

[0138] Control effect (%) = (disease index of blank control area - disease index of control area) / disease index of blank control area × 100

[0139] The results are shown in Table 5:

[0140] Table 5 Test results of each treatment agent for controlling wheat scab

[0141]

[0142] As can be seen from Table 5, the (15+3)% fluorophenyletheramide·thiophanate-methyl suspension has a good control effect on wheat fusarium rust after application, reaching 88.62%, and has no adverse effects on the growth and filling period of wheat, so it can be promoted and used in production.

[0143] Example 6: Field efficacy test of the combination of fluphenazine ether amide and thiamethoxam against strawberry gray mold

[0144] Overview of the test site: The test site was selected in the greenhouse of the strawberry planting base in Daotian Town, Shouguang, Shandong. The soil is fertile, the terrain is flat, and the fertility is uniform. The test crop variety is "Frandi", which was planted in December 2021. During the test, the cultivation conditions and management measures of all plots were uniform and consistent, and followed local production practices.

[0145] Experimental treatment: The experimental agent was the (15+3)% fluorophenyletheramide·thiophanate-methyl suspension of Preparation Example 3, and the control agent was 5% thiophanate-methyl suspension (commercially available, Shaanxi Xida Huate Technology Industry Co., Ltd.), 15% fluorophenyletheramide suspension (laboratory self-made sample, Preparation Example 10), and the blank control was sprayed with an equal amount of clean water. The experiment adopted a randomized block design, with a total of 4 treatments, staggered. Each treatment was repeated 3 times, with a total of 12 plots, and the area of ​​each plot was 50m 2 The rated spray dosage for each plot is 1.5 kg. The pesticide was applied twice at 4 pm on April 10 and April 17, 2022. The weather was sunny and windless on the day of application, and the temperature was 13-22°C. The pesticide machine used was Linong 15L (HD-400 model) backpack sprayer, which was sprayed evenly according to the conventional spraying method until a small amount of water dripped from the leaves.

[0146] Survey method: Before the first application of pesticides (April 10), investigate the incidence of gray mold of strawberries in each treatment area, calculate the disease index before application, and investigate the incidence of gray mold of strawberries in each treatment area 7 days after the second application of pesticides (April 24). Each plot adopts five-point fixed-point sampling on the diagonal line, and 5 plants are surveyed at each point. The incidence of all fruits of each strawberry plant is recorded, the incidence level is recorded, the disease index and prevention effect are calculated, and the safety of the pesticide on strawberries is investigated. The disease index and the prevention and control effect of the pesticide are calculated based on the degree of damage and the symptoms. The grading standards are as follows:

[0147] Level 0: no disease;

[0148] Level 1: The lesion area accounts for less than 5% of the entire fruit surface;

[0149] Level 3: The lesion area accounts for 6% to 15% of the entire fruit surface;

[0150] Level 5: The lesion area accounts for 16% to 25% of the entire fruit surface;

[0151] Level 7: The lesion area accounts for 26% to 50% of the entire fruit surface;

[0152] Level 9: The lesion area accounts for more than 50% of the entire fruit surface.

[0153] Then, the disease index and control effect of each treatment are calculated as follows:

[0154] Diseased fruit rate (%) = (number of diseased fruits / total number of investigated fruits) × 100

[0155] Disease index = 100 × ∑ (number of diseased fruits at each level × relative level value) / (total number of fruits investigated × 9)

[0156] Control effect (%) = [1-(CK0×PT1) / (CK1×PY0)]×100

[0157] CK0: disease index of blank control area before drug application;

[0158] CK1: disease index of blank control area after application;

[0159] PT0: disease index before application of pesticide in the treatment area;

[0160] PT1: Disease index after pesticide application in the pesticide treatment area.

[0161] The results are shown in Table 6:

[0162] Table 6 Test results of each treatment agent for controlling strawberry gray mold

[0163]

[0164]

[0165] As can be seen from Table 6, the (15+3)% fluphenazine·thiophanate-methyl suspension has a good control effect on strawberry gray mold after application, reaching 87.82%, and has no adverse effects on strawberries at any growth stage, so it can be promoted and used in production.

[0166] The synergistic composition containing fluorophenyl ether amide and thiophanate-methyl can be prepared into any dosage form suitable for agricultural use by known methods, and the suspension is preferably prepared by test screening. When the ratio of fluorophenyl ether amide and thiophanate-methyl is 15:1 to 1:15, it shows obvious synergistic effect on rice sheath blight, wheat fusarium head blight and strawberry gray mold, especially when the ratio of the two is 5:1, the control effect of the above three diseases is the best, the synergistic effect is significant, and secondly, there is no adverse effect on the target crop during use.

[0167] Based on the above ideal embodiments of the present invention, through the description of the above cases, relevant personnel can make various changes and modifications without departing from the technical concept of the present invention. The technical scope of the present invention is not limited to the content in the specification. The technical scope must be determined according to the scope of the claims to select a more suitable dosage form and control target.

Claims

1. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl, characterized in that: The active ingredients of the composition are fluphenazine amide and thiophanate-methyl, wherein the weight ratio of fluphenazine amide to thiophanate-methyl is 15:1 to 1:

15.

2. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl according to claim 1, characterized in that: The bactericidal composition is composed of the following components in percentage by weight: 1% to 30% of thiophanate-methyl, 1% to 85% of fluorophenyletheramide, and the rest are adjuvants allowed to be used and added in pesticide science.

3. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl according to claim 2, characterized in that: The total weight percentage of the fluorophenyletheramide and thiophanate-methyl in the composition is 1% to 75%.

4. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl according to claim 3, characterized in that: The total weight percentage of the fluorophenyletheramide and thiophanate-methyl in the composition is 1% to 65%.

5. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl according to claim 2, characterized in that: The auxiliary agents allowed to be used and added in the pesticide science are selected from solvents, penetrants, dispersants, diffusing agents, disintegrants, emulsifiers, stabilizers, wetting agents, thickeners, defoamers, and antifreeze agents.

6. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl according to claim 2, characterized in that: The bactericidal composition is prepared into a suspension, a wettable powder, a water-dispersible granule, a granule, an aqueous emulsion, a microemulsion or an ultra-low volume liquid.

7. A bactericidal composition containing fluorophenyletheramide and thiophanate-methyl according to claim 4, characterized in that: The bactericidal composition is prepared as a suspension.

8. Use of the fungicidal composition according to any one of claims 1 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that: The plant diseases include rice sheath blight, wheat fusarium rust and strawberry gray mold.

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