A compound preparation for improving the selenium-enriching capacity of peanut sprouts, and a preparation method and application thereof

Abstract

The application provides a compound preparation for improving selenium enrichment capacity of peanut sprouts, and a preparation method and application thereof. The compound preparation takes water as a solvent, and contains the following components in each liter of the compound preparation: 3-30 mg of sodium selenite, 9-360 mg of 10-HDA, 0.5-5.0 g of ammonium gluconate, and 0.5-15 g of pullulan. The compound preparation can effectively increase the fresh weight of peanut sprouts, promote the growth of hypocotyls, reduce the proportion of root fresh weight in total fresh weight, and improve the selenium content and the proportion of organic selenium of peanut sprouts. In addition, the components are simple, and the compound preparation is suitable for large-scale production and application.

Description

Technical Field

[0001] This invention relates to a compound preparation for improving the selenium enrichment capacity of peanut sprouts, its preparation method and application, belonging to the field of selenium-enriched agricultural product production technology. Background Technology

[0002] Sprouts are a common type of vegetable. The germination process of legumes optimizes the nutritional structure of the seeds, increasing the content of active ingredients and making them more beneficial to human health. Soybean sprouts and mung bean sprouts have a long history of consumption, but in recent years, peanut sprouts have emerged as a new type of vegetable, gradually gaining recognition and popularity due to their high nutritional value. Germination of peanut seeds hydrolyzes the large protein molecules in the seeds into amino acids, making them easier for the body to absorb. During germination, oils are converted into energy or degraded from saturated fatty acids into unsaturated fatty acids, significantly reducing the fat content. At the same time, germination increases the content of vitamins and minerals such as potassium, calcium, iron, and zinc. Nutritionally balanced bean sprouts are very beneficial to human health.

[0003] The Chinese Nutrition Society recommends a daily selenium intake of 50-200 μg per person. However, some people's daily selenium intake falls far short of this recommendation. Diseases caused by insufficient selenium intake pose a significant threat to human health, such as Kashin-Beck disease, Keshan disease, malignant tumors, and weakened immunity. For a long time, some selenium-deficient areas in my country have tried to alleviate the problem by adding inorganic sodium selenite to food. However, inorganic selenium has low bioavailability and is somewhat toxic. Converting it into organic selenium through certain methods can overcome the drawbacks of inorganic selenium.

[0004] Peanuts are a good biological carrier for selenium enrichment. Most of the selenium in peanut kernels exists in the form of protein-bound selenium, with lower amounts in polysaccharide-bound and fat-bound selenium. Because peanuts contain a large amount of protein, the protein-bound selenium content in selenium-enriched peanuts can reach up to 73.39% of the total organic selenium content, demonstrating that peanut protein has a stronger selenium-enriching capacity than other compounds such as polysaccharides and nucleic acids. Existing selenium enrichment research mainly focuses on harvesting selenium-containing peanut kernels, while reports on utilizing the intense physiological metabolic activity during peanut germination and growth to increase selenium accumulation in peanut seedlings and thus harvest selenium-enriched peanut sprouts are still limited. Enriching germinating peanut seeds with selenium to cultivate novel selenium-enriched peanut sprout functional vegetables, as a highly efficient selenium supplement, can be used to improve the health of selenium-deficient populations. Using inorganic selenium to enrich organic selenium through the biotransformation of peanut seedlings is an effective method for cultivating selenium-enriched peanut sprouts; the mechanism of selenium enrichment is closely related to the antioxidant system and secondary metabolism of peanut seedlings. Sodium selenite, as an additive in selenium-containing fertilizers, has been used for selenium enrichment of agricultural products. However, plants respond inconsistently to different concentrations of sodium selenite. Soils with excessively high selenium content can be toxic to selenium-sensitive crops, manifesting as growth inhibition, wilting, and drying. When preparing sprouts using the same concentration of sodium selenite, peanut sprouts show significantly lower selenium enrichment capacity than soybean sprouts, mung bean sprouts, and other vegetables. Furthermore, the ratio of organic to inorganic selenium in peanut sprouts needs further improvement. However, further increasing the selenium concentration in the environment may stress the growth of peanut sprouts.

[0005] Pullulan is a linear polysaccharide composed of repeating maltotriose units linked by α-1,4 glycosidic bonds and polymerized by α-1,6 glycosidic bonds. The higher molecular weight components exhibit stronger film-forming, gas-barrier, and plasticity properties, while the applications of the lower molecular weight components require further exploration. Studies have shown that this sugar can alleviate the inhibitory effect of water stress on maize growth and, under severe drought conditions, can increase the activity of soil sucrase and urease.

[0006] Fatty acids and their derivatives, as a unique class of bioactive substances, have a wide range of functions in biological systems. Royal jelly acid (10-hydroxy-2-decenoic acid, 10-HDA) is a medium-chain unsaturated fatty acid secreted by the mandibular glands of bees and is a characteristic product of royal jelly. 10-HDA has antioxidant, immune-enhancing, anti-inflammatory, antibacterial, antitumor, lipid-lowering, and blood pressure-lowering effects, and is widely used in pharmaceuticals, health products, and cosmetics. In addition to chemical synthesis, 10-HDA has now been synthesized microbially using inexpensive decanoic acid as a substrate.

[0007] Ammonium gluconate contains both ammonium ions and gluconate ions. Studies have shown that it can alleviate drought and salt stress in plants, promote seed germination, and enhance the activity of antioxidant enzymes in plants.

[0008] Therefore, it is worthwhile to further study which compound preparations can effectively improve the selenium enrichment capacity of peanut sprouts, increase the ratio of organic to inorganic selenium in peanut sprouts, and promote the growth of peanut sprouts. Summary of the Invention

[0009] To address the shortcomings of existing technologies, this invention provides a compound preparation for enhancing the selenium enrichment capacity of peanut sprouts, along with its preparation method and application. This compound preparation effectively increases the fresh weight of peanut sprouts, promotes hypocotyl growth, reduces the proportion of root fresh weight in the total fresh weight, and increases the selenium content and organic selenium content of peanut sprouts. Furthermore, its components are simple and suitable for large-scale production and application.

[0010] The technical solution of the present invention is as follows:

[0011] A compound preparation for improving the selenium enrichment capacity of peanut sprouts, using water as a solvent, comprises the following components per liter: sodium selenite 3-30 mg, 10-HDA 9-360 mg, ammonium gluconate 0.5-5.0 g, and pullulanose 0.5-15 g.

[0012] According to a preferred embodiment of the present invention, the compound preparation uses water as a solvent, and each liter of the compound preparation comprises the following components: sodium selenite 5-25 mg, 10-HDA 18-180 mg, ammonium gluconate 1.5-4.5 g, pullulanose 1.5-10 g.

[0013] Preferably, the compound preparation uses water as a solvent, and each liter of the compound preparation includes the following components: sodium selenite 10-20 mg, 10-HDA 36-90 mg, ammonium gluconate 2.0-3.0 g, pullulanose 2.5-5 g.

[0014] Most preferably, the compound preparation uses water as a solvent, and each liter of the compound preparation contains the following components: sodium selenite 15mg, 10-HDA 90mg, ammonium gluconate 2.0g, and pullulanose 5g.

[0015] According to the present invention, the ammonium gluconate is commercially available or prepared using existing technology.

[0016] According to the present invention, pullulanose has a molecular weight of 0.34 kDa to 100 kDa.

[0017] Preferably, pullulanose has a molecular weight of 0.9kDa to 20kDa.

[0018] Further preferred, pullulanose has a molecular weight of 1.2kDa to 10.0kDa, with the most preferred value being 10kDa.

[0019] The preparation method of the above-mentioned compound preparation for improving the selenium enrichment capacity of peanut sprouts includes the following steps: adding dimethyl sulfoxide to 10-HDA until completely dissolved, then adding water, ammonium gluconate, sodium selenite and pullulanose, and mixing and dispersing them evenly to obtain the compound preparation.

[0020] According to a preferred embodiment of the present invention, the concentration of dimethyl sulfoxide in the compound formulation is less than or equal to 0.1 wt%.

[0021] The application of the above-mentioned compound preparation for improving the selenium enrichment capacity of peanut sprouts is to enhance the selenium enrichment capacity of peanut sprouts, promote the growth of peanut sprouts, and improve the edible quality of peanut sprouts.

[0022] According to a preferred embodiment of the present invention, the application method includes the following steps: disinfecting peanut seeds with a 2-4% H2O2 aqueous solution and rinsing them with distilled water; soaking them in distilled water at room temperature for 10-14 hours and draining the water; then culturing them at 20-25°C under the action of the compound preparation; during the culturing period, replacing the compound preparation with a new one every two days.

[0023] Preferably, the ratio of peanut seeds to compound preparation is 1L of compound preparation to treat 400-500 peanut seeds.

[0024] The technical features and beneficial effects of this invention are as follows:

[0025] 1. The pullulan used in the compound formulation of this invention is a low molecular weight pullulan, which can act as a biostimulant, improve plant stress resistance, alleviate the stress caused by high concentrations of sodium selenite on plant growth, and effectively increase the total selenium content and organic selenium content in peanut sprouts. Pullulan can also increase the content of soluble sugars and soluble proteins in peanut sprouts, promote hypocotyl growth, and improve the edible quality of peanut sprouts. Furthermore, only the specific type of pullulan used in this invention can simultaneously achieve the excellent effects mentioned in this invention; if other sugars such as sucrose are used instead, the effects are significantly reduced. The molecular weight of the pullulan in this invention needs to be appropriate. If the molecular weight is too high, the film-forming property increases, causing it to adhere to the seed surface. Furthermore, if the molecular weight is too high or too low, its activity as a biostimulant will decrease, reducing its ability to alleviate the stress caused by sodium selenite on growth and increase the total selenium content and organic selenium content in peanut sprouts. The dosage of pullulan in this invention needs to be appropriate. If the dosage is too low, it will not have the desired effect; if the dosage is too high, the production cost will increase and it will cause osmotic stress on peanut sprout growth.

[0026] 2. The 10-HDA used in the compound formulation of this invention can participate in the regulation of multiple defense pathways in plants, such as basal immunity, effector-induced resistance, and systemic resistance, thereby improving the plant's resistance to abiotic stress. The 10-HDA used in this invention can increase the total selenium content and organic selenium content in peanut sprouts, promote hypocotyl growth, and improve the edible quality of peanut sprouts. The dosage of 10-HDA in this invention needs to be appropriate; too low or too high a dosage will not produce ideal results.

[0027] 3. The ammonium gluconate used in the compound formulation of this invention can provide nitrogen and carbon nutrients while improving plant stress resistance. The carbon source provides raw materials for the synthesis of various organic carbon skeletons in plants, thus facilitating the synthesis of organic selenium. The ammonium gluconate of this invention can increase the total selenium content and organic selenium content in peanut sprouts, promote hypocotyl growth, reduce the proportion of root fresh weight in the total fresh weight, thereby increasing the proportion of edible portion and improving the edible quality of peanut sprouts. The dosage of ammonium gluconate in this invention needs to be appropriate; too low a dosage will not have a significant effect, while too high a dosage will inhibit plant growth. If ammonium carbonate (which contains both carbon and nitrogen sources) is used instead, it will not be able to increase the total selenium content and organic selenium content in peanut sprouts, nor will it promote hypocotyl growth.

[0028] 4. The amount of sodium selenite used in this invention needs to be appropriate. If the amount is too low, the selenium content in peanut sprouts will be low, and if the amount is too high, it will cause stress to the growth of peanut sprouts.

[0029] 5. The present invention is a compound preparation with specific types and compositions of raw materials. Pullulanose, 10-HDA, and ammonium gluconate work synergistically, while pullulanose, 10-HDA, ammonium gluconate, and sodium selenite play a synergistic role, thereby increasing the organic selenium content of peanut sprouts while improving the yield and edible quality of the sprouts.

[0030] 6. The raw materials of the compound formulation of the present invention are simple, the preparation method is simple and efficient, and it is conducive to large-scale production and application. Detailed Implementation

[0031] The technical solution of the present invention will be further described below with reference to the embodiments, but the scope of protection of the present invention is not limited thereto.

[0032] Unless otherwise specified, all raw materials used in the embodiments are conventional products; unless otherwise specified, all equipment used are conventional equipment.

[0033] The ammonium gluconate used was prepared according to the method in Example 2 of patent document CN109022507A, with a purity of 97%; the sodium selenite, 10-HDA, and pullulanose used can be commercially available or prepared according to existing technology.

[0034] Example 1

[0035] A compound preparation for improving the selenium enrichment capacity of peanut sprouts, wherein the compound preparation uses water as a solvent and contains 30 mg of sodium selenite, 360 mg of 10-HDA, 5 g of ammonium gluconate, 15 g of pullulanose, and pullulanose with a molecular weight of 50 kDa per liter of compound preparation.

[0036] The preparation steps are as follows: slowly add a small amount of dimethyl sulfoxide to 10-HDA until it is just completely dissolved, then add water to 1L, and after dilution, control the concentration of dimethyl sulfoxide to within 0.1wt%. Then dissolve ammonium gluconate, sodium selenite and pullulanose in water and stir to disperse them evenly.

[0037] Disinfect peanut seeds with a 3% (w / w) H2O2 aqueous solution for 10 minutes, then rinse 5 times with distilled water; soak the seeds in distilled water at room temperature for 12 hours, then drain the water; then place them in a germination machine containing the compound preparation of this invention for 5 days (place the seeds on the germination tray of the germination machine, and spray the compound preparation from the water tank at the bottom along the spray pipe once per hour. The sprayed compound preparation can return to the water tank through the holes on the germination tray for recycling. 1L of compound preparation can treat 400-500 seeds), with a cultivation temperature of 20-25℃, and replace the compound preparation treatment solution once on the 3rd day.

[0038] Cultured according to the above method, with only sodium selenite added (i.e., water as solvent, containing only 30 mg of sodium selenite per liter of preparation) as the control group.

[0039] The physiological indicators of peanut sprouts obtained from the above experiment were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 9.3%, the length of the hypocotyl increased by 5.1%, the total selenium content of peanut sprouts increased by 16.5%, of which the organic selenium content increased by 50.8%, the soluble sugar content increased by 22.1%, and the soluble protein increased by 7.6%. This indicates that the compound preparation in this embodiment can effectively improve the selenium enrichment capacity of peanut sprouts.

[0040] Example 2

[0041] A compound preparation for improving the selenium enrichment capacity of peanut sprouts, wherein the compound preparation uses water as a solvent and contains 25 mg of sodium selenite, 180 mg of 10-HDA, 1.5 g of ammonium gluconate, 10 g of pullulanose, and pullulanose with a molecular weight of 20 kDa per liter.

[0042] The preparation steps are as follows: slowly add a small amount of dimethyl sulfoxide to 10-HDA until it is just completely dissolved, then add water to 1L, and after dilution, control the concentration of dimethyl sulfoxide to within 0.1wt%. Then dissolve ammonium gluconate, sodium selenite and pullulanose in water and stir to disperse them evenly.

[0043] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 25 mg of sodium selenite per liter of the preparation).

[0044] The physiological indicators of peanut sprouts obtained from the above experiment were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 17.2%, the length of the hypocotyl increased by 9.6%, the total selenium content of peanut sprouts increased by 18.7%, of which the organic selenium content increased by 61.5%, the soluble sugar content increased by 32.4%, and the soluble protein increased by 13.8%. This indicates that the compound preparation in this embodiment can effectively improve the selenium enrichment capacity of peanut sprouts.

[0045] Example 3

[0046] A compound preparation for improving the selenium enrichment capacity of peanut sprouts, wherein the compound preparation uses water as a solvent and contains 3 mg of sodium selenite, 9 mg of 10-HDA, 0.5 g of ammonium gluconate, 0.5 g of pullulanose per liter of compound preparation, with a pullulanose molecular weight of 0.34 kDa.

[0047] The preparation steps are as follows: slowly add a small amount of dimethyl sulfoxide to 10-HDA until it is just completely dissolved, then add water to 1L, and after dilution, control the concentration of dimethyl sulfoxide to within 0.1wt%. Then dissolve ammonium gluconate, sodium selenite and pullulanose in water and stir to disperse them evenly.

[0048] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 3 mg of sodium selenite per liter of the preparation).

[0049] The physiological indicators of peanut sprouts obtained from the above experiment were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 7.1%, the length of the hypocotyl increased by 3.9%, the total selenium content of peanut sprouts increased by 10.2%, of which the organic selenium content increased by 25.3%, the soluble sugar content increased by 16.5%, and the soluble protein increased by 6.9%. This indicates that the compound preparation in this embodiment can effectively improve the selenium enrichment capacity of peanut sprouts.

[0050] Example 4

[0051] A compound preparation for improving the selenium enrichment capacity of peanut sprouts, wherein the compound preparation uses water as a solvent and contains 15 mg of sodium selenite, 90 mg of 10-HDA, 2 g of ammonium gluconate, 5 g of pullulanose, and pullulanose with a molecular weight of 10 kDa per liter of compound preparation.

[0052] The preparation steps are as follows: slowly add a small amount of dimethyl sulfoxide to 10-HDA until it is just completely dissolved, then add water to 1L, and after dilution, control the concentration of dimethyl sulfoxide to within 0.1wt%. Then dissolve ammonium gluconate, sodium selenite and pullulanose in water and stir to disperse them evenly.

[0053] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 15 mg of sodium selenite per liter of the preparation).

[0054] The physiological indicators of peanut sprouts obtained from the above experiment were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 21.5%, the length of the hypocotyl increased by 12.7%, the total selenium content of peanut sprouts increased by 22.3%, of which the organic selenium content increased by 84.8%, the soluble sugar content increased by 62.9%, and the soluble protein increased by 21.8%. This indicates that the compound preparation in this embodiment can effectively improve the selenium enrichment capacity of peanut sprouts.

[0055] Example 5

[0056] A compound formulation, as described in Example 4, except that pullulanose has a molecular weight of 100 kDa.

[0057] The compound preparation uses water as a solvent, and each liter of the compound preparation contains 15 mg of sodium selenite, 90 mg of 10-HDA, 2 g of ammonium gluconate, 5 g of pullulanose, and the molecular weight of pullulanose is 100 kDa.

[0058] The preparation steps are as described in Example 4.

[0059] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 15 mg of sodium selenite per liter of the preparation).

[0060] The physiological indicators of peanut sprouts obtained from the above experiments were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 8.3%, the length of the hypocotyl increased by 5.1%, the total selenium content of peanut sprouts increased by 10.2%, of which the organic selenium content increased by 40.6%, the soluble sugar content increased by 26.5%, and the soluble protein increased by 11.8%. The culture effect of the compound preparation with the changed pullulan molecular weight of selenium-enriched peanut sprouts in this example was not as good as that in Example 4.

[0061] Comparative Example 1

[0062] A compound formulation, as described in Example 4, except that 10-HDA is not added.

[0063] The compound preparation uses water as a solvent, and each liter of the compound preparation contains 15 mg of sodium selenite, 2 g of ammonium gluconate, 5 g of pullulanose, and the pullulanose has a molecular weight of 10 kDa.

[0064] The preparation steps are as described in Example 4.

[0065] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 15 mg of sodium selenite per liter of the preparation).

[0066] The physiological indicators of the peanut sprouts obtained in the above experiment were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 16.4%, the length of the hypocotyl increased by 9.3%, the total selenium content of the peanut sprouts increased by 20.1%, of which the organic selenium content increased by 81.7%, the soluble sugar content increased by 56.2%, and the soluble protein increased by 19.3%. The comparative compound preparation lacked 10-HDA and the culture effect of selenium-enriched peanut sprouts was not as good as that of Example 4.

[0067] Comparative Example 2

[0068] A compound formulation, as described in Example 4, except that ammonium gluconate is replaced with ammonium carbonate.

[0069] The compound preparation uses water as a solvent, and each liter of the compound preparation contains 15 mg of sodium selenite, 90 mg of 10-HDA, 2 g of ammonium carbonate, 5 g of pullulanose, and pullulanose has a molecular weight of 10 kDa.

[0070] The preparation steps are as described in Example 4.

[0071] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 15 mg of sodium selenite per liter of the preparation).

[0072] The physiological indicators of peanut sprouts obtained from the above experiments were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 10.4%, the length of the hypocotyl increased by 5.6%, the total selenium content of peanut sprouts increased by 12.1%, of which the organic selenium content increased by 40.2%, the soluble sugar content increased by 26.7%, and the soluble protein increased by 13.8%. The comparative compound preparation, which replaced ammonium gluconate with ammonium carbonate, did not have the same effect on the cultivation of selenium-enriched peanut sprouts as in Example 4.

[0073] Comparative Example 3

[0074] A compound formulation, as described in Example 4, except that pullulanose is replaced with sucrose.

[0075] The compound preparation uses water as a solvent, and each liter of the compound preparation contains 15 mg of sodium selenite, 90 mg of 10-HDA, 2 g of ammonium gluconate, and 5 g of sucrose.

[0076] The preparation steps are as described in Example 4.

[0077] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 15 mg of sodium selenite per liter of the preparation).

[0078] The physiological indicators of peanut sprouts obtained from the above experiments were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 10.7%, the length of the hypocotyl increased by 6.5%, the total selenium content of peanut sprouts increased by 13.8%, of which the organic selenium content increased by 51.9%, the soluble sugar content increased by 29.5%, and the soluble protein increased by 15.9%. The comparative compound preparation, which replaced pullulanose with sucrose, did not have the same effect on the cultivation of selenium-enriched peanut sprouts as in Example 4.

[0079] Comparative Example 4

[0080] A compound formulation, as described in Example 4, except that 10-HDA is replaced with decanoic acid.

[0081] The compound preparation uses water as a solvent, and each liter of the compound preparation contains 15 mg of sodium selenite, 90 mg of decanoic acid, 2 g of ammonium gluconate, 5 g of pullulanose, and pullulanose has a molecular weight of 10 kDa.

[0082] The preparation steps are as described in Example 4.

[0083] Peanut sprouts were cultivated according to the method in Example 1; a control group was prepared by adding only sodium selenite (i.e., using water as a solvent, with only 15 mg of sodium selenite per liter of the preparation).

[0084] The physiological indicators of peanut sprouts obtained from the above experiments were measured. The results showed that compared with the control group, the fresh weight of the hypocotyl increased by 17.2%, the length of the hypocotyl increased by 10.1%, the total selenium content of peanut sprouts increased by 21.3%, of which the organic selenium content increased by 80.2%, the soluble sugar content increased by 58.3%, and the soluble protein increased by 18.9%. The comparative compound preparation, which replaced 10-HDA with selenium-enriched decanoic acid, did not have the same effect on peanut sprout cultivation as in Example 4.

Claims

1. A compound preparation for improving the selenium enrichment capacity of peanut sprouts, characterized in that, Using water as a solvent, each liter of the compound preparation contains the following components: sodium selenite 10~20 mg, 10-HDA 36~90 mg, ammonium gluconate 2.0~3.0 g, pullulanose 2.5~5 g; pullulanose molecular weight 1.2kDa~10.0 kDa.

2. The compound preparation for improving the selenium enrichment capacity of peanut sprouts according to claim 1, characterized in that, The compound preparation uses water as a solvent and contains the following components per liter: sodium selenite 15 mg, 10-HDA 90 mg, ammonium gluconate 2.0 g, pullulanose 5 g.

3. The compound preparation for improving the selenium enrichment capacity of peanut sprouts according to claim 1, characterized in that, Pullulan has a molecular weight of 10 kDa.

4. A method for preparing the compound preparation for improving the selenium enrichment capacity of peanut sprouts as described in any one of claims 1-3, comprising the steps of: Dimethyl sulfoxide was added to 10-HDA until it was completely dissolved. Then water, ammonium gluconate, sodium selenite and pullulanose were added and mixed thoroughly to obtain a compound preparation.

5. The method for preparing the compound preparation for improving the selenium enrichment capacity of peanut sprouts according to claim 4, characterized in that, In the compound formulation, the concentration of dimethyl sulfoxide is less than or equal to 0.1 wt%.

6. The application of the compound preparation for improving the selenium enrichment capacity of peanut sprouts as described in any one of claims 1-3, which is used to improve the selenium enrichment capacity of peanut sprouts, promote the growth of peanut sprouts, and improve the edible quality of peanut sprouts.