Preparation and application of broccoli extract

Abstract

The present invention relates to a method for preparing a broccoli extract and its use in controlling spider mites. The broccoli extract is obtained by enzymatically hydrolyzing broccoli using an endogenous enzyme system, extracting and concentrating the active ingredients in the broccoli using a solvent, and finally drying the broccoli extract. The broccoli extract can be used to prepare acaricides.

Description

Technical Field

[0001] The invention belongs to the field of pesticides, and particularly relates to the preparation and application of a broccoli extract. Background Art

[0002] The cinnabarinus spider mite, scientifically known as Tetranychus cinnabarinus (Boisduval), is also known as the cotton red spider, red spider mite, and rose red spider mite. It is a worldwide pest, infesting over 113 plant species and harming 18 vegetable varieties, including eggplant, beans, melons, tomatoes, peppers, potatoes, and onions. It is a major pest not only in fields but also in protected and greenhouse vegetable cultivation. It is widespread in South China, Northwest my country, Southwest China, and Northeast China. Adults, larvae, and nymphs of the cinnabarin spider mite feed on the undersides of leaves, increasing transpiration, damaging chloroplasts, and inhibiting photosynthesis. Leaves can redden, curl, wither, and fall off, and even die entirely.

[0003] Traditional methods for controlling Tetranychus cinnabarinus mites rely primarily on chemical acaricides such as avermectins and pyrethroids. However, these issues, such as the development of resistance in mites due to long-term use, ecological pollution and damage caused by excessive use, and the accumulation of chemicals in various organisms, pose serious health risks to humans. These issues are attracting increasing attention from scientists. In recent years, natural products, such as plant extracts, have become a hot topic in insecticide research due to their natural origin and minimal environmental impact.

[0004] Broccoli is rich in various nutrients and bioactive compounds, such as vitamins, minerals, fiber, glucosinolates, and phenolic compounds, with varying concentrations in different parts. Studies have shown that broccoli and its derivatives possess a variety of benefits, including antioxidant, anti-inflammatory, anticancer, antibacterial, metabolic disorder regulation, neuroprotection, and renal protection. However, there are no reports on the antiparasitic activity of broccoli and its derivatives, particularly against Tetranychus cinnabarinus. The present invention provides a method for preparing a broccoli extract and its use in preparing acaricides. Summary of the Invention

[0005] The present invention aims to provide a preparation method of a broccoli extract and application of the broccoli extract in preventing and controlling spider mites.

[0006] The present invention relates to a method for preparing a broccoli extract, which comprises the following steps:

[0007] (1) crushing at least one of the stems, leaves, and florets of broccoli;

[0008] (2) The crushed broccoli is enzymatically hydrolyzed by an endogenous enzyme system;

[0009] (3) adding a solvent to the enzymatically hydrolyzed broccoli for extraction and filtering to obtain a filtrate;

[0010] (4) The filtrate is dried to obtain broccoli extract.

[0011] The enzymatic hydrolysis conditions of the endogenous enzyme system in step (2) above are as follows: temperature is 30±10°C; time is 60±20 minutes.

[0012] The solvent in step (3) is one or more of methanol, ethanol and acetone.

[0013] The present invention also relates to the use of the broccoli extract for controlling Tetranychus cinnabarinus. In one embodiment, the broccoli extract exhibits strong acaricidal activity and has the potential to be developed as an acaricide. The acaricide can be formulated as a water-dispersible granule, emulsifiable concentrate, aqueous suspension concentrate, oil suspension concentrate, microemulsion, or tablet.

[0014] The following examples are intended to further illustrate the present invention but are not intended to limit the present invention. DETAILED DESCRIPTION

[0015] The following examples are provided to further illustrate the present invention but are not intended to limit the present invention. Based on the examples in the present invention, all other examples obtained by persons of ordinary skill in the art without creative work are within the scope of protection of the present invention.

[0016] Example 1 Preparation of broccoli extract

[0017] The broccoli florets were crushed with a grinder, and 1 kg was placed at 30°C for 60 minutes. 2 volumes of ethanol were added and stirred for 30 minutes. The filtrate was filtered with gauze to obtain 1.0 L of filtrate. The filtrate was vacuum concentrated to dryness at 55°C to obtain 31 g of broccoli extract 1.

[0018] Example 2 Preparation of Broccoli Extract

[0019] 1 kg of broccoli stems and leaves were crushed with a grinder, then placed at 30°C for 60 minutes, and then 2 volumes of ethanol were added and stirred for 30 minutes. The filtrate was filtered with gauze to obtain 1.2 L of filtrate, and the filtrate was vacuum concentrated to dryness at 55°C to obtain 27 g of broccoli extract 2.

[0020] Example 3 Preparation of Broccoli Extract

[0021] The whole broccoli was crushed with a grinder, and then 1 kg was taken and placed at 30°C for 60 minutes, and then 2 volumes of ethanol were added and stirred for 30 minutes. The filtrate was filtered with gauze to obtain 1.1 L of filtrate, and the filtrate was vacuum concentrated to dryness at 55°C to obtain 30 g of broccoli extract 3.

[0022] Example 4 Preparation of Broccoli Extract

[0023] 20% Tween was added to the broccoli extract 1, broccoli extract 2 and broccoli extract 3 in Examples 1-3, respectively, and then diluted with water to obtain a preparation with a broccoli extract concentration of 5 wt%.

[0024] Example 5 Biological activity of broccoli extract against Tetranychus cinnabarinus

[0025] The activity of broccoli extract against Tetranychus cinnabarinus was determined using the stem-dip method developed by the Southern Pesticide Innovation Center. A blank control was prepared using the same method as in Example 4.

[0026] Adult Tetranychus cinnabarinus mites were inoculated onto prepared broad bean leaves, maintaining a mite population of 30-60. The inoculated broad bean leaves were immersed in the drug solution for 5 seconds, then removed and placed into a conical flask filled with clean water. This process was repeated three times per treatment. The treated insects were cultured under normal conditions, their growth regularly observed, and the number of live and dead insects in each treatment was determined after 24 hours. The experimental results showed that Broccoli Extract 1, Broccoli Extract 2, and Broccoli Extract 3 had a control efficacy of 98.2%, 95.6%, and 97.4%, respectively.

Claims

1. Use of broccoli extract for controlling Tetranychus cinnabarinus, the preparation method of the broccoli extract comprising the following steps: (1) at least one of the stems, leaves and florets of broccoli is crushed; (2) the crushed broccoli is subjected to enzymatic hydrolysis by an endogenous enzyme system at a hydrolysis temperature of 30±10°C and a hydrolysis time of 60±20 minutes; (3) the hydrolyzed broccoli is extracted with a solvent and filtered to obtain a filtrate, wherein the solvent is selected from one or more of methanol, ethanol and acetone; (4) the filtrate is concentrated under reduced pressure to obtain a broccoli extract.

2. Use of the broccoli extract according to claim 1 for preparing acaricide.