A production and planting method of pleurotus nebroseus
By optimizing the culture medium formula and treatment methods, the problems of low fruiting rate and long growth cycle in the cultivation of Pleurotus eryngii were solved, achieving efficient mushroom growth and quality improvement.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- XUCHANG SHIJIXIANG BIOTECH CO LTD
- Filing Date
- 2023-06-30
- Publication Date
- 2026-06-26
AI Technical Summary
Existing methods for cultivating Pleurotus eryngii result in low fruiting rates, poor quality of individual mushrooms, and long growth cycles.
The culture medium formula includes carbon sources (such as potato juice and sucrose) and nitrogen sources (such as wheat bran powder and ammonium nitrate). Combined with shaking and temperature control, mycelial balls are prepared and then sprayed onto solid culture medium to optimize the inoculum growth conditions.
It improved the fruiting rate of the spawn and the growth quality of individual mushrooms, shortened the growth cycle, and enhanced the overall quality of Pleurotus eryngii.
Smart Images
Figure BDA0004314770810000071 
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Abstract
Description
Technical Field
[0001] This invention relates to the field of mushroom cultivation, and particularly to a method for producing and cultivating Pleurotus eryngii. Background Technology
[0002] Pleurotus eryngii, also known as white ferruginosa mushroom, is an edible fungus. When harvesting, select mushrooms that are palmate in shape, intact, with thick, fresh, and plump caps, and a pure white color. These mushrooms are of higher quality, resulting in better-quality finished products and greater profits for growers. Simultaneously, shortening the cultivation cycle is crucial to further increase profits. Therefore, shortening the cultivation cycle and improving product quality are particularly important during the cultivation of Pleurotus eryngii.
[0003] In the current cultivation process of Pleurotus eryngii, sawdust, corn cobs, cottonseed hulls, etc. are usually used as culture media. After high-temperature sterilization, inoculation is carried out. The mycelium needs to grow for one or two months. It goes through two-stage cultivation of primary spawn and production spawn before it can be used as a cultivation spawn. The growth cycle is relatively slow. At the same time, since all the raw materials in the culture medium are in powder form, these raw materials are prone to caking during use after being mixed evenly. This reduces the porosity of the culture medium, which hinders and limits the growth of mushrooms.
[0004] In the aforementioned related technologies, the inventors believe that existing methods for cultivating Pleurotus eryngii can easily lead to a decrease in the fruiting rate of the spawn and poor growth quality of individual mushrooms. Summary of the Invention
[0005] In order to improve the fruiting rate and growth quality of individual mushrooms during the cultivation of Pleurotus eryngii, this application provides a method for the production and cultivation of Pleurotus eryngii.
[0006] Firstly, this application provides a method for the production and cultivation of Pleurotus eryngii, which adopts the following technical solution:
[0007] A method for producing and cultivating Pleurotus eryngii mainly includes the following steps:
[0008] S1: Prepare a culture medium, sterilize the culture medium, and inoculate the white lingzhi mushroom spawn into the sterilized culture medium to cultivate and prepare mycelial balls; the culture medium includes a carbon source, a nitrogen source, water, and inorganic salts; the carbon source is at least one of potato juice and sucrose; the nitrogen source is at least one of wheat bran powder and ammonium nitrate;
[0009] S2: Spray the mycelial ball solution obtained in step S1 onto a solid culture medium to prepare a pre-culture medium;
[0010] S3: Cultivate the pre-culture medium obtained in step S2 to promote mycelial growth, and then harvest and package the mushrooms.
[0011] By adopting the above technical solution, a culture medium is first prepared, ensuring that nutrients are evenly distributed and do not easily accumulate. The spawn is then planted in the culture medium, allowing for full contact and absorption of nutrients, accelerating spawn reproduction and growth, and shortening the growth cycle of *Pleurotus eryngii*. The carbon source, as the source of carbon elements required for spawn growth, is the main raw material for maintaining spawn vitality and aiding in spawn metabolism. Potato juice, rich in starch and vitamins, can promote spawn growth and reproduction, increasing the subsequent fruiting rate and resulting in larger, thicker mushrooms, thus improving the quality of individual *Pleurotus eryngii* mushrooms. Sucrose contains a large amount of nutrients that can be directly utilized by the spawn. Carbon can quickly provide a large amount of carbon to the Pleurotus eryngii spawn, promoting its growth. Wheat bran, as an organic nitrogen source, contains a relatively high amount of crude protein, sugar, and vitamins, making it nutritious and promoting spawn growth, thus increasing the subsequent fruiting rate. Ammonium nitrate, as an inorganic nitrogen source, allows the nitrogen in its form to be quickly utilized by the Pleurotus eryngii spawn, accelerating its growth and subsequently improving the quality of individual Pleurotus eryngii mushrooms. The spawn is first cultured in a nutrient solution to prepare a mycelial ball solution rich in mycelial balls, which is then sprayed onto a solid culture medium. This increases the fruiting rate of the spawn on the solid medium while also shortening the spawn's growth time and improving the quality of individual Pleurotus eryngii mushrooms.
[0012] Optionally, in step S1, during the process of cultivating and preparing mycelial ball solution, the culture solution containing Pleurotus eryngii spawn is shaken and rotated at a speed of 130-180 r / min.
[0013] By employing the above-mentioned technical solution, the culture medium is oscillated and rotated, allowing the spawn to inoculate and grow into mycelial balls within the rotating culture medium, thereby improving the growth rate and survival rate of the spawn. Adjusting the rotation rate of the culture medium is crucial; a slower rotation rate results in a lower growth rate and survival rate, affecting the subsequent fruiting rate and quality of the Pleurotus eryngii. Conversely, a faster rotation rate leads to larger diameter mycelial balls, which are difficult to control and hinder subsequent inoculation into solid culture media, thus impacting the fruiting rate and quality of individual Pleurotus eryngii mushrooms.
[0014] Optionally, the temperature for incubating the pre-culture medium in step S3 is 15-30℃.
[0015] By adopting the above technical solution and adjusting the temperature of the pre-culture medium, the growth rate of mycelial balls increases with the increase of temperature. However, when the temperature is too high, the mycelial balls are easily heated, which leads to a decrease in their vitality and growth rate, thus affecting the growth quality of Pleurotus eryngii.
[0016] Optionally, the carbon source in step S1 is composed of potato juice and sucrose in a mass ratio of (3-4):(1-2).
[0017] By adopting the above technical solution, both potato juice and sucrose are good carbon sources required for the growth of Pleurotus eryngii. While providing abundant carbon sources, potato juice also contains a lot of vitamins, which can provide more nutrients for Pleurotus eryngii. However, the absorption rate of carbon sources in potato juice by the spores is relatively slow. Sucrose is a pure carbon source, and the carbon sources in it can be directly utilized by the Pleurotus eryngii spores more quickly. Therefore, with the further combined effect of potato juice and sucrose, the growth and reproduction rate of the spores can be accelerated, promoting the subsequent growth of Pleurotus eryngii.
[0018] Optionally, the nitrogen source in step S1 is composed of wheat bran powder and ammonium nitrate in a mass ratio of (2-4):(2-3).
[0019] By adopting the above technical solutions, wheat bran can provide organic nitrogen source for the growth of Pleurotus eryngii spawn, as well as trace elements, promoting the growth and yield of Pleurotus eryngii. However, wheat bran itself is prone to the growth of Aspergillus flavus, which can contaminate the Pleurotus eryngii spawn and cause nutritional deficiencies during the spawn's growth, making it unsuitable for large-scale use. Ammonium nitrate, as an inorganic nitrogen source, can be quickly absorbed by the Pleurotus eryngii spawn, promoting the fruiting and growth of the spawn. By adding ammonium nitrate and wheat bran, organic and inorganic nitrogen sources are combined, allowing Pleurotus eryngii to better absorb nitrogen for growth, promoting subsequent growth and improving the quality of individual Pleurotus eryngii mushrooms.
[0020] Optionally, the culture medium contains a growth promoter; the mass ratio of the growth promoter to the carbon source is 1:(9-11); the preparation method of the growth promoter includes the following steps:
[0021] (1) Soak corn stalks in lime water, then take them out and pile them up for 2-3 days to obtain pre-fermented corn stalks;
[0022] (2) Mix dried cow dung with water and pile it up for 2-3 days to obtain pre-fermented dried cow dung;
[0023] (3) Mix the pre-fermented corn stalks obtained in step (1) and the pre-fermented dried cow manure obtained in step (2) to obtain a mixture; mix the mixture with water, boil, and filter out the solids to obtain the final product.
[0024] By adopting the above technical solution, corn stalks and dried cow manure are processed and then piled up for fermentation. During the fermentation process, nutrients are produced to prepare pre-fermented corn stalks and pre-fermented dried cow manure. Then, the pre-fermented corn stalks and pre-fermented dried cow manure are mixed and boiled to kill pathogens and insect eggs in the pre-fermented corn stalks and pre-fermented dried cow manure, thus preparing a growth-promoting solution. The growth-promoting solution is added to the culture medium to improve the nutritional components in the culture medium, further promoting the growth of the spores and forming mycelial balls. The number of fluffy mycelia on the surface of the generated mycelial balls increases, enhancing the vitality of the mycelial balls. At the same time, it can also provide "parasitic nuclei" for the formation of mycelial balls, improving the subsequent growth of mycelial balls on solid culture media, further improving the fruiting rate of mycelial balls, and also improving the growth quality of subsequent Pleurotus eryngii.
[0025] Optionally, the mass ratio of the mixture to water in step (3) is (6-12):100.
[0026] By adopting the above technical solution and adjusting the mass ratio of the mixture and water, the concentration of corn stalks and pre-fermented dried cow manure in the prepared growth-promoting solution can be adjusted. Adding the growth-promoting solution can enhance the vitality of mycelial balls, promote their growth, and improve the fruiting rate and quality of subsequent Pleurotus eryngii. However, when the amount of mixture added is too large, the diameter of the mycelial balls generated by the spawn is difficult to control, and larger diameter mycelial balls are not conducive to inoculation into solid culture media. When the amount of mixture added is too small, the promoting effect on spawn growth is insufficient, affecting the rate and diameter of mycelial ball generation, and thus affecting the subsequent growth of Pleurotus eryngii.
[0027] Optionally, the solid culture medium in step S2 is composed of sawdust, corn cob powder, wheat bran, calcium carbonate and lime in a mass ratio of (36-44):(43-50):(6-9):(0.5-1.5):(3-5).
[0028] By adopting the above technical solution, adding sawdust to the solid culture medium allows the *Pleurotus eryngii* spawn to secrete cellulase, which converts the cellulose in the sawdust into glucose for absorption and utilization, promoting the growth of *Pleurotus eryngii* mycelial balls and increasing the yield of *Pleurotus eryngii*. Corn cob powder contains a large amount of active substances, which can also promote the growth of *Pleurotus eryngii* mycelial balls. Wheat bran contains a large amount of vitamins, including vitamins B1, B2, B6, and B12, as well as vitamin E, which can further promote the mycelial growth of *Pleurotus eryngii* mycelial balls. Adding calcium percarbonate provides calcium for mycelial ball growth. Adding lime adjusts the pH of the solid culture medium to alkalinity, increasing its alkalinity, inhibiting the growth of mold in the solid culture medium, providing metal ions for mycelial ball growth, and promoting the growth of *Pleurotus eryngii*. Through the combination of raw materials in the solid culture medium, the growth of mycelial balls and *Pleurotus eryngii* is promoted, thus improving the quality of the *Pleurotus eryngii* mushroom.
[0029] Optionally, in step S2, before spraying the mycelial ball solution onto the solid culture medium, the solid culture medium is heated to 55-60°C and maintained for 6-8 hours, then ventilated and cooled to 45-55°C, and maintained for 4-6 days to obtain the final product.
[0030] By adopting the above technical solution, the solid culture medium is heated, and the high temperature further decomposes the complex organic matter in the solid culture medium. At the same time, it kills insect eggs and spores of miscellaneous bacteria and pathogens in the solid culture medium. This reduces the damage of miscellaneous bacteria and insect eggs to the mycelial balls after they are planted in the solid culture medium, increases the fruiting rate of the mycelial balls, and also promotes the growth of the subsequent white lingzhi mushrooms, thus improving the growth quality of white lingzhi mushrooms.
[0031] In summary, this application has the following beneficial effects:
[0032] This application involves first preparing a culture medium, then adding the spawn to the culture medium. This allows the nutrients in the culture medium to fully contact and be absorbed by the spawn, promoting rapid growth and reproduction of the spawn. The spawn then quickly grows into mycelial balls, which are then inoculated onto a solid culture medium. This results in a high fruiting rate, fast growth rate, short growth cycle, and high quality of Pleurotus eryngii. Detailed Implementation
[0033] The present application will be further described in detail below with reference to embodiments and comparative examples;
[0034] Unless otherwise specified, the raw materials used in the embodiments and comparative examples of this application are all commercially available.
[0035] Preparation Example
[0036] Example 1 of preparation of growth promoter
[0037] The preparation method of the growth-promoting solution in this example includes the following steps:
[0038] (1) Take a reaction bucket, put lime water in the reaction bucket, soak the corn stalks in the lime water for 10 minutes, take them out, put them in a hard, flat, non-water-accumulating place, and pile them up at room temperature and away from light for 2.5 days to obtain pre-fermented corn stalks; the mass concentration of quicklime in the lime water used is 15%;
[0039] (2) Take the reaction bucket again, crush the dried cow manure and add it together with water into the reaction bucket. After mixing evenly, select a hard, flat site that does not accumulate water, and pile it up for 2.5 days under normal temperature and light-proof conditions to obtain pre-fermented dried cow manure. The mass ratio of dried cow manure to water used is 5:1.
[0040] (3) Take a mixing bucket, place the pre-fermented corn stalks obtained in step (1) and the pre-fermented dry cow manure obtained in step (2) into the mixing bucket, and mix them evenly according to a mass ratio of 3:2 to obtain a mixture; mix the mixture with water, boil it, and filter out the solids to obtain the mixture; the mass ratio of the mixture to water used is 6:100.
[0041] Example 2 of preparation of growth promoter
[0042] The difference between this growth promoter preparation example and the growth promoter preparation example 1 is that the mass ratio of the mixture to water used in step (3) is 12:100.
[0043] Example 3 of preparation of growth promoter
[0044] The difference between this growth promoter preparation example and the growth promoter preparation example 1 is that the mass ratio of the mixture to water used in step (3) is 9:100.
[0045] Example
[0046] Example 1
[0047] The production and cultivation method of Pleurotus eryngii in this embodiment mainly includes the following steps;
[0048] S1: Take a reaction flask and place the carbon source, nitrogen source, water, and potassium dihydrogen phosphate together in the flask. Mix well to prepare a 1-liter culture medium. After sterilizing the culture medium at high temperature, dispense it into shake flasks, each containing 250 ml of culture medium. The total volume of the shake flasks is 1000 ml. Inoculate the shake flasks with Pleurotus eryngii spawn, using 4 Pleurotus eryngii spawn blocks per shake flask, each with an area of 3 mm * 3 mm. Inoculate the Pleurotus eryngii spawn blocks into the culture medium and place the shake flasks at 25°C for three days to prepare mycelial ball solution. The mass ratio of carbon source, nitrogen source, water, and potassium dihydrogen phosphate used is 6:4:10:0.6. The carbon source used is sucrose, and the nitrogen source used is ammonium nitrate.
[0049] S2: Place the solid culture medium in the liquid inoculation room. Transport the mycelial ball solution obtained in step S1 to the liquid inoculation room via a sterile automated pipeline. Spray the mycelial ball solution onto the solid culture medium, with 35 ml of mycelial ball solution sprayed onto each solid culture medium to prepare a pre-culture medium. The solid culture medium is composed of sawdust, corn cob powder, wheat bran, calcium carbonate, and lime in a mass ratio of 40:47:8:1:4. Each solid culture medium weighs 5 kg. The temperature in the liquid inoculation room is 27℃. S3: Place the pre-culture medium from step S2 in a cultivation room and incubate in the dark for 25 days to allow mycelial growth and fruiting. The temperature in the cultivation room is 15℃, and the humidity is 70%.
[0050] Example 2
[0051] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in embodiment 1 is that the carbon source used in step S1 is potato juice and the nitrogen source is ammonium nitrate.
[0052] Example 3
[0053] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in Embodiment 1 is that the carbon source used in step S1 is potato juice, and the nitrogen source used is wheat bran powder.
[0054] Example 4
[0055] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in embodiment 3 is that, in step S1, after the spawn is inoculated into the culture solution and cultivated into mycelial balls, the culture solution is placed in a constant temperature shaker for oscillation and rotation at a speed of 130 r / min.
[0056] Example 5
[0057] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in embodiment 3 is that, after the spawn is inoculated into the culture solution in step S1, during the cultivation of mycelial balls, the culture solution is placed in a constant temperature shaker for oscillation and rotation at a speed of 180 r / min.
[0058] Example 6
[0059] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in embodiment 3 is that, after the spawn is inoculated into the culture solution in step S1, during the cultivation of mycelial balls, the culture solution is placed in a constant temperature shaker for shaking at a rotation speed of 160 r / min.
[0060] Example 7
[0061] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in embodiment 6 is that the temperature of the pre-culture medium placed in the culture room for mycelial growth in step S3 is 30°C.
[0062] Example 8
[0063] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in embodiment 6 is that the temperature at which the pre-culture medium is placed in the culture room for mycelial growth in step S3 is 20°C.
[0064] Example 9
[0065] The production and cultivation method of Pleurotus eryngii in this embodiment differs from that in embodiment 8 in that the carbon source used in step S1 is composed of potato juice and sucrose in a mass ratio of 3.5:1.5.
[0066] Example 10
[0067] The production and cultivation method of Pleurotus eryngii in this embodiment differs from that in Example 9 in that the nitrogen source used in step S1 is composed of wheat bran powder and ammonium nitrate in a mass ratio of 3:2.5.
[0068] Example 11
[0069] The production and cultivation method of Pleurotus eryngii in this embodiment differs from that in Example 10 in that, before spraying the mycelial ball liquid into the solid culture medium in step S2, the solid culture medium is first heated to 58°C and maintained for 7 hours, and then ventilated to cool the solid culture medium to 50°C and maintained for 5 days.
[0070] Example 12
[0071] The difference between the production and cultivation method of Pleurotus eryngii in this embodiment and that in Example 11 is that a growth promoter is added to the culture medium in step S1, and the mass ratio of the growth promoter to the carbon source is 1:10; the growth promoter is prepared in Example 1 of growth promoter preparation.
[0072] Example 13
[0073] The production and cultivation method of Pleurotus eryngii in this embodiment differs from that in Example 11 in that the culture medium in step S1 contains a growth promoter, and the mass ratio of the growth promoter to the carbon source is 1:10; the growth promoter is prepared in Example 2 of growth promoter preparation.
[0074] Example 14
[0075] The production and cultivation method of Pleurotus eryngii in this embodiment differs from that in Example 11 in that the culture medium in step S1 contains a growth promoter, and the mass ratio of the growth promoter to the carbon source is 1:10; the growth promoter is prepared in Example 3 of growth promoter preparation.
[0076] Comparative Example
[0077] Comparative Example 1
[0078] The production and cultivation methods of Pleurotus eryngii in this comparative example mainly include the following steps:
[0079] S1: Take the inoculum blocks, mix 4 inoculum blocks with an area of 3mm*3mm with 250ml of water to prepare the inoculum solution;
[0080] S2: Place the solid culture medium in the liquid inoculation room. Transport the mycelial ball solution obtained in step S1 to the liquid inoculation room via a sterile automated pipeline. Spray the mycelial ball solution onto the solid culture medium, with 35 ml of mycelial ball solution sprayed onto each solid culture medium to prepare a pre-culture medium. The solid culture medium is composed of sawdust, corn cob powder, wheat bran, calcium carbonate, and lime in a mass ratio of 40:47:8:1:4. Each solid culture medium weighs 5 kg. The temperature in the liquid inoculation room is 27℃. S3: Place the pre-culture medium from step S2 in a cultivation room and incubate in the dark for 25 days to allow mycelial growth and fruiting. The temperature in the cultivation room is 15℃, and the humidity is 70%.
[0081] Comparative Example 2
[0082] The difference between the production and cultivation method of Pleurotus eryngii in this comparative example and that in Example 1 is that the carbon source used in step S1 is glucose.
[0083] Comparative Example 3
[0084] The difference between the production and cultivation method of Pleurotus eryngii in this comparative example and that in Example 1 is that the nitrogen source used in step S1 is urea.
[0085] Detection methods
[0086] Pleurotus eryngii was prepared according to the production and cultivation methods of Examples 1-14 and Comparative Examples 1-3. The average weight of a single Pleurotus eryngii mushroom in a solid culture medium and the bioconversion rate in the solid culture medium were measured and calculated, and the data were recorded as shown in Table 1. The test method for bioconversion rate in solid culture medium includes the following steps:
[0087] Bioconversion rate (%) = dry weight of mycelium and dry weight of carbon and nitrogen sources in the culture medium × 100; dry weight of carbon and nitrogen sources in the culture medium = weight of carbon source × (1 - water content of carbon source %) + weight of nitrogen source × (1 - water content of nitrogen source %).
[0088] Table 1. Average weight and bioconversion rate of Pleurotus eryngii prepared in Examples 1-14 and Comparative Examples 1-3.
[0089]
[0090]
[0091] As can be seen from Examples 1-10, Comparative Examples 1-3, and Table 1, preparing the culture medium first facilitates full contact and absorption of nutrients between the spawn and the substrate, resulting in rapid spawn reproduction and growth, high-quality Pleurotus eryngii, and a short growth cycle. After inoculating the spawn into the culture medium, the medium is shaken. Too high or too low a rotation speed is detrimental to spawn cultivation. A rotation speed of 160 r / min effectively promotes contact between the spawn and nutrients in the culture medium, allowing the spawn to grow into mycelial balls of suitable diameter, facilitating subsequent inoculation into a solid culture medium for further growth into Pleurotus eryngii with high mushroom quality. After the Pleurotus eryngii mycelial balls are planted in the solid culture medium, the inoculation temperature is adjusted. Table 1 compares the quality of Pleurotus eryngii mushrooms; both excessively high and low temperatures are unsuitable for growth. At 20℃, the growth of Pleurotus eryngii is optimal, with the highest average weight per mushroom and the best quality.
[0092] By adjusting the ratio of carbon and nitrogen sources in the culture medium, when the carbon source consists of potato juice and sucrose in a mass ratio of 3.5:1.5, the potato juice can provide more carbon for the growth of the spawn, as well as abundant vitamins. Combined with sucrose, the carbon in the sucrose can be quickly utilized by the spawn, helping it grow into mycelial balls, thus improving the average quality of the subsequently grown Pleurotus eryngii. Simultaneously, when the nitrogen source consists of wheat bran and ammonium nitrate in a mass ratio of 3:2.5, the wheat bran provides organic nitrogen for the spawn, while the ammonium nitrate provides inorganic nitrogen, both assisting in the growth and reproduction of the spawn into mycelial balls, further improving the average quality of the subsequently grown Pleurotus eryngii.
[0093] In addition, compared with Example 11, before spraying the mycelial ball liquid into the solid culture medium, the solid culture medium is first subjected to high temperature treatment to remove insect eggs, miscellaneous bacteria and pathogens in the solid culture medium, thereby improving the growth rate and fruiting rate of the mycelial balls on the solid culture medium and further improving the average quality of Pleurotus eryngii.
[0094] As can be seen from Implementations 12-14 and Table 1, after separately fermenting corn stalks and dried cow dung, they are mixed together to prepare a mixture. After mixing with water and boiling, and filtering, a growth-promoting liquid rich in cow dung fermentation products and corn stalk fermentation products is obtained. Adjusting the ratio of the mixture to water in the growth-promoting liquid allows it to be added to the culture medium, which can improve the nutritional components in the culture medium and further promote the growth of the spores into mycelial balls with better vitality. This makes it easier to subsequently plant the mycelial balls in solid culture medium, improve the fruiting rate of the mycelial balls, and also improve the average quality of the grown Pleurotus eryngii.
[0095] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A method for producing and cultivating Pleurotus eryngii, characterized in that: The main steps include the following: S1: Prepare a culture medium, sterilize the culture medium, and inoculate the white lingzhi mushroom spawn into the sterilized culture medium to cultivate and prepare mycelial balls; the culture medium includes a carbon source, a nitrogen source, water, and inorganic salts; the carbon source is at least one of potato juice and sucrose; the nitrogen source is at least one of wheat bran powder and ammonium nitrate; S2: Spray the mycelial ball solution obtained in step S1 onto a solid culture medium to prepare a pre-culture medium; S3: Cultivate the pre-culture medium obtained in step S2 to promote mycelial growth, and the mushrooms will be obtained. The carbon source mentioned in step S1 is composed of potato juice and sucrose in a mass ratio of (3-4):(1-2); The nitrogen source mentioned in step S1 is composed of wheat bran powder and ammonium nitrate in a mass ratio of (2-4):(2-3); The culture medium contains a growth promoter; the mass ratio of the growth promoter to the carbon source is 1:(9-11); the preparation method of the growth promoter includes the following steps: (1) Soak corn stalks in lime water, then take them out and pile them up for 2-3 days to obtain pre-fermented corn stalks; (2) Mix dried cow dung with water and pile it up for 2-3 days to obtain pre-fermented dried cow dung; (3) Mix the pre-fermented corn stalks obtained in step (1) and the pre-fermented dried cow manure obtained in step (2) to obtain a mixture; mix the mixture with water, boil, and filter out the solids to obtain the final product.
2. The method for producing and cultivating Pleurotus eryngii according to claim 1, characterized in that: In step S1, during the process of cultivating and preparing mycelial ball solution, the culture solution containing Pleurotus eryngii spawn is shaken and rotated at a speed of 130-180 r / min.
3. The method for producing and cultivating Pleurotus eryngii according to claim 2, characterized in that: The temperature for incubating the pre-culture medium in step S3 is 15-30℃.
4. The method for producing and cultivating Pleurotus eryngii according to claim 1, characterized in that: The mass ratio of the mixture to water in step (3) is (6-12):
100.
5. The method for producing and cultivating Pleurotus eryngii according to claim 1, characterized in that: The solid culture medium described in step S2 is composed of sawdust, corn cob powder, wheat bran, calcium carbonate and lime in a mass ratio of (36-44):(43-50):(6-9):(0.5-1.5):(3-5).
6. The method for producing and cultivating Pleurotus eryngii according to claim 5, characterized in that: Before spraying the mycelial ball solution onto the solid culture medium in step S2, the solid culture medium is heated to 55-60℃ and maintained for 6-8 hours. Then, it is ventilated and cooled to 45-55℃ and maintained for 4-6 days.