Composition with anti-oxidation function and skin care product

Abstract

The present invention belongs to the technical field of cosmetics, and discloses a composition with antioxidant function and a skin care product. The composition with antioxidant function comprises the following components: white truffle extract, black truffle extract, peach blossom extract, and yeast extract. The present invention provides a composition with antioxidant function, which produces a good synergistic effect through the compounding of white truffle extract, black truffle extract, peach blossom extract, and yeast extract. The compounding of these four extracts has a significant improvement in overall antioxidant and uniform skin color effects; compared with the use of a single component, the compounding of two or three components has a more obvious improvement in antioxidant effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition with anti-oxidation function and a skin care product. Background Art

[0002] Antioxidant is the abbreviation of antioxidant free radicals. The human body continuously produces free radicals in the body due to continuous contact with the outside world, including breathing (oxidation reaction), external pollution, radiation exposure and other factors. Scientific research shows that cancer, aging or other diseases are mostly related to the production of excessive free radicals. Research on antioxidants can effectively overcome the harm it brings, so antioxidants are listed as one of the main research and development directions by health care products and cosmetics companies, and are also one of the most important functional demands in the market.

[0003] Black truffle, also known as truffle, truffle mushroom, pig arch mushroom and motherless fruit, is a general term for a type of mushroom, belonging to the genus truffle of the genus truffle of the phylum Ascomycota, and is a kind of precious edible fungus that coexists with trees for both medicinal and edible purposes. It is mainly distributed in Yunnan, Sichuan, Xinjiang and Tibet in my country, and in Italy, France and Spain in Europe. Studies have shown that black truffles are rich in polysaccharides, proteins, amino acids and polyunsaturated fatty acids. At the same time, they are also rich in minerals, sterols, flavonoids, mannitol and vitamin C, which makes black truffle extracts have good free radical scavenging ability and iron ion chelating ability, and have a significant effect in inhibiting oxidative stress damage.

[0004] Truffles cannot photosynthesize by themselves to produce carbohydrates for growth. Therefore, they must live in symbiosis with the roots of oak trees and use the roots of oak trees to absorb nutrients. Therefore, the growth range of truffles is generally in the jungles where trees gather. A large number of differentiated hyphae are called mycelium. When all the necessary environments are available, some hyphae will intertwine with each other to form spore-containing fruiting bodies, which are truffles. When they are discovered and eaten by mammals, they can use mammals to spread their spores. White truffles contain 28 sulfur-containing compounds and a large number of isoprenes, which is the secret of the aroma of the magical fruit. The anti-inflammatory effect of white truffles is mainly manifested in the inhibition of cyclooxygenase (COX-2). Cyclooxygenase is an oxygenase that plays an important role in the body's inflammation process, so white truffles have a certain anti-inflammatory effect.

[0005] Both black truffles and white truffles have certain antioxidant effects.

[0006] Based on the above-mentioned effects of black truffles and white truffles, many formulas in the cosmetics industry have been developed around black truffles or white truffles to produce some skin care products with high skin care value.

[0007] For example, D1: Chinese patent 202211134664.8 discloses an anti-aging composition, which is prepared by compounding yeast / acetobacter xylinum / black tea fermentation products, lactobacillus / ginseng root fermentation product filtrate, acetyl hexapeptide-8, Dendrobium officinale extract, collagen, bosacein, retinoic acid esters, white truffle extract and matsutake extract. The anti-aging composition of the invention is prepared by scientifically combining plant fermentation products, plant extracts and a variety of anti-aging ingredients. Experiments have found that the anti-aging effect of each component raw material is not obvious when used alone, but after compounding with specific raw materials, the anti-aging effect is significant, and there is a synergistic effect.

[0008] The above patent discloses that white truffle extract can be used as an anti-aging ingredient, but also points out that the effect is not obvious when used alone. White truffle extract can also be used as an antioxidant ingredient. Therefore, the above patent provides some research and development directions, and a skin care composition with good antioxidant effect can be formulated around white truffle extract.

[0009] And D2: Chinese patent 202110674915.0 discloses a moisturizing lotion and a preparation method thereof, comprising the following components: thickener, polyol, moisturizer, pH regulator, niacinamide, tranexamic acid, skin conditioner and preservative; the skin conditioner comprises the following components: Tremella extract, Otimine, oat polypeptide, Magnolia sieboldii extract, black truffle extract, white truffle extract, bifid yeast fermentation product lysate, Eucystis extract, water-soluble retinol, hydrolyzed yeast protein and hydrolyzed sericin; wherein, Otimine is a mixture of water, lactobacillus / soy milk fermentation product filtrate, butanediol and 1,2-pentanediol. The lotion of this invention can improve the moisturizing degree of BB cream or air cushion cream, make the makeup smoother, and make the skin more delicate and moisturized.

[0010] The above patent discloses a technical solution for using black truffle and white truffle in combination with other components. By compounding black truffle and white truffle with other extracts and skin care components, the skin can be made more delicate and moisturized; however, the above patent does not conduct research and development on the antioxidant effect of the compound components of black truffle and white truffle, so it can only be used as a reference for ideas.

[0011] Based on the above reasons, the applicant needs to develop a compound composition based on black truffle extract and white truffle extract so as to significantly enhance the antioxidant effect of the composition. Summary of the invention

[0012] One of the purposes of the present invention is to provide a composition with antioxidant function to solve the problem that there is no compound around black truffle and white truffle in the prior art, and to develop a skin care composition that improves the antioxidant effect of the skin, fill the gap in the market, and have a high market application prospect.

[0013] Another object of the present invention is to provide a skin care product, which contains the above-mentioned composition with antioxidant function. The antioxidant effect of the compound of multiple extracts is significantly improved compared with the use of a single component, and can effectively improve the antioxidant capacity of the skin.

[0014] To achieve the above object, the present invention provides a composition with antioxidant function, comprising the following components: white truffle extract, black truffle extract, peach blossom extract, and yeast extract.

[0015] Preferably, the following components are included by weight percentage: 20-40% white truffle extract, 20-40% black truffle extract, 20-40% peach blossom extract, and 20-40% yeast extract.

[0016] Furthermore, the weight percentage of the white truffle extract can be selected as 20%, 25%, 30%, 35%, or 40%.

[0017] Furthermore, the weight percentage of the black truffle extract can be selected as 20%, 25%, 30%, 35%, or 40%.

[0018] Furthermore, the weight percentage of the peach blossom extract can be selected as 20%, 25%, 30%, 35%, or 40%.

[0019] Furthermore, the weight percentage of the yeast extract can be selected to be 20%, 25%, 30%, 35%, or 40%.

[0020] The present invention also provides a skin care product, comprising the above-mentioned composition with antioxidant function, wherein the content of the composition with antioxidant function is ≥3%.

[0021] Preferably, the content of the composition having antioxidant function is 3.0-8.0%.

[0022] Further preferably, the content of the composition with antioxidant function can be selected as 3.0%, 3.5%, 4.0%, 4.5%, 5.0%, 5.5%, 6.0%, 6.5%, 7.0%, 7.5%, and 8.0%.

[0023] Beneficial Effects

[0024] Compared with the prior art, the present invention has at least the following advantages:

[0025] (1) The present invention provides a composition with antioxidant function, which produces a good synergistic effect through the compounding of white truffle extract, black truffle extract, peach blossom extract and yeast extract. The black truffle extract has antioxidant activity, can effectively reduce the excessive accumulation of reactive oxygen, reduce the generation of oxidative stress damage, thereby reducing organelle and cell damage, and avoiding the generation of chronic inflammation in the local microenvironment; the white truffle extract has an anti-inflammatory effect, can reduce the intensity and continuous spread of the reaction of the inflammation that has already occurred, reduce inflammatory damage, and thus reduce the local pigmentation caused by inflammatory damage; the peach blossom extract can reduce the generation of free radicals while also reducing the accumulation of oxidation products, while protecting cells and improving the survival rate of cells in a polluted and oxidatively damaged environment; the yeast extract can promote the initiation of cell autophagy, help the skin degrade waste proteins and organelles caused by existing damage, thereby making the skin uniform in skin color and maintaining a healthy circulation; the compounding of these four extracts has a benefit, and the overall antioxidant and uniform skin color effects are greatly improved; compared with the use of a single component, the compounding of two or three of the components has a more obvious improvement in antioxidant efficacy;

[0026] (2) The present invention limits the compounding ratio of white truffle extract, black truffle extract, peach blossom extract and yeast extract. Through compounding optimization, the antioxidant effect of the composition is further enhanced, and the skin has a uniform skin tone.

[0027] (3) When the composition disclosed in the present invention is used in skin care products, when the content is lower than 3%, the skin antioxidant effect of the product is not obvious, and it needs to be higher than 3%. When the content is higher than 8%, the skin repair effect of the product is not significantly improved, so the preferred content range is 3-8%. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0029] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0030] In the following examples and comparative examples, the white truffle extract, black truffle extract, peach blossom extract, gentian extract, bifid yeast lysate, camellia extract, and ginseng extract were all purchased from Guangzhou Jianeng Biotechnology Co., Ltd., and the yeast extract was purchased from Silab, France.

[0031] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0032] Unless otherwise specified, % and part in the present invention refer to percent by weight and part by weight.

[0033] Example 1

[0034] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of white truffle extract, 30 parts of black truffle extract, 20 parts of peach blossom extract and 20 parts of yeast extract.

[0035] Example 2

[0036] A composition with anti-oxidation function is obtained by uniformly mixing 20 parts of white truffle extract, 40 parts of black truffle extract, 20 parts of peach blossom extract and 20 parts of yeast extract.

[0037] Example 3

[0038] A composition with anti-oxidation function is obtained by uniformly mixing 25 parts of white truffle extract, 25 parts of black truffle extract, 25 parts of peach blossom extract and 25 parts of yeast extract.

[0039] Example 4

[0040] A composition with anti-oxidation function is obtained by uniformly mixing 40 parts of white truffle extract, 20 parts of black truffle extract, 20 parts of peach blossom extract and 20 parts of yeast extract.

[0041] Example 5

[0042] A composition with anti-oxidation function is obtained by uniformly mixing 20 parts of white truffle extract, 20 parts of black truffle extract, 40 parts of peach blossom extract and 20 parts of yeast extract.

[0043] Example 6

[0044] A composition with anti-oxidation function is obtained by uniformly mixing 20 parts of white truffle extract, 20 parts of black truffle extract, 20 parts of peach blossom extract and 40 parts of yeast extract.

[0045] Example 7

[0046] A composition with anti-oxidation function is obtained by uniformly mixing 50 parts of white truffle extract, 20 parts of black truffle extract, 20 parts of peach blossom extract and 10 parts of yeast extract.

[0047] Example 8

[0048] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of white truffle extract, 10 parts of black truffle extract, 50 parts of peach blossom extract and 10 parts of yeast extract.

[0049] Comparative Example 1

[0050] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of white truffle extract, 30 parts of black truffle extract, 20 parts of peach blossom extract and 20 parts of bifid yeast lysate.

[0051] Comparative Example 2

[0052] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of gentian extract, 30 parts of black truffle extract, 20 parts of peach blossom extract and 20 parts of yeast extract.

[0053] Comparative Example 3

[0054] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of white truffle extract, 30 parts of ginseng extract, 20 parts of peach blossom extract and 20 parts of yeast extract.

[0055] Comparative Example 4

[0056] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of white truffle extract, 30 parts of black truffle extract, 20 parts of camellia extract and 20 parts of yeast extract.

[0057] Comparative Example 5

[0058] A composition with anti-oxidation function is obtained by uniformly mixing 30 parts of white truffle extract, 50 parts of camellia extract and 20 parts of yeast extract.

[0059] Comparative Example 6

[0060] A composition with anti-oxidation function is obtained by uniformly mixing 50 parts of ginseng extract, 30 parts of black truffle extract and 20 parts of yeast extract.

[0061] Comparative Example 7

[0062] A composition with anti-oxidation function is obtained by uniformly mixing 45 parts of black truffle extract, 28 parts of peach blossom extract and 27 parts of yeast extract.

[0063] Comparative Example 8

[0064] A composition with anti-oxidation function is obtained by uniformly mixing 45 parts of white truffle extract, 27 parts of peach blossom extract and 28 parts of yeast extract.

[0065] Comparative Example 9

[0066] A composition with antioxidant function is obtained by uniformly mixing 37.5 parts of white truffle extract, 37.5 parts of black truffle extract and 25 parts of yeast extract.

[0067] Comparative Example 10

[0068] A composition with antioxidant function is obtained by uniformly mixing 37.5 parts of white truffle extract, 37.5 parts of black truffle extract and 25 parts of peach blossom extract.

[0069] Results Test

[0070] 1. Zebrafish ROS fluorescence test

[0071] 1.1 Experimental Background

[0072] Reactive oxygen species (ROS) are chemically reactive species containing oxygen. In the human body, ROS are formed as a natural byproduct of normal oxygen metabolism and play an important role in cell signaling and homeostasis. Exogenous stimuli (such as ultraviolet or heat exposure) and endogenous problems (such as aging) can cause the balance of ROS generation and clearance to be disrupted, resulting in wrinkles and other problems on the human skin.

[0073] The fluorescent probe DCFH-DA itself has no fluorescence and can freely pass through the cell membrane. After entering the cell body, it can be hydrolyzed by esterase in the cell to generate DCFH, while DCFH cannot pass through the cell membrane and will be retained in the cell body. When excessive reactive oxygen accumulates in the cell body, DCFH will be oxidized to generate the fluorescent substance DCF. Therefore, the fluorescence intensity of DCF represents the content of reactive oxygen in the cell.

[0074] 1.2 Experimental Setup

[0075] The fluorescent probe DCFH-DA itself has no fluorescence and can freely pass through the cell membrane. After entering the cell body, it can be hydrolyzed by esterase in the cell to generate DCFH, while DCFH cannot pass through the cell membrane and will be retained in the cell body. When excessive reactive oxygen accumulates in the cell body, DCFH will be oxidized to generate the fluorescent substance DCF. Therefore, the fluorescence intensity of DCF represents the content of reactive oxygen in the cell.

[0076] 1.3 Experimental methods

[0077] The zebrafish were randomly divided into blank control, model, and sample groups. The blank group was added with standard diluent, the model group was added with modeling agent, and the sample group was added with a mixture of modeling agent and sample. After 24 hours of constant temperature culture, the embryos of each group were washed and fluorescent probes were added.

[0078] The content of reactive oxygen species in fish was quantitatively detected by fluorescence labeling.

[0079] (1) Treatment of control and test samples

[0080] Blank control group: standard dilution water

[0081] Sample group: Composition with a concentration of 1%

[0082] (2) Test operation steps

[0083] Normally developed embryos were randomly selected for the experiment, and a 24-well cell culture plate was used as the experimental carrier. The prepared test sample dilutions of different concentrations were added to each well of the 24-well cell culture plate, and the plate was covered and then placed in an incubator for 24 hours. Three parallel groups were set for each concentration group, and a blank control group was also set.

[0084] Add 2 ml of fluorescent probe to each well, let it stand in a constant temperature box for 2 hours, wash the fish embryos, and take pictures under a fluorescence microscope.

[0085] (3) Calculation formula

[0086] Calculation of reactive oxygen species (ROS) clearance rate:

[0087] Clearance rate = (average value of blank control group - average value of sample group) / average value of blank control group.

[0088] (4) Data analysis

[0089] Open the photo with analysis software such as Image J, and select the yolk area of ​​each fish embryo to analyze the ROS fluorescence intensity.

[0090] The results are shown in Table 1;

[0091] Table 1 Fluorescence test results of the compositions with antioxidant function of Examples 1-8 and Comparative Examples 1-10

[0092]

[0093]

[0094] According to the results in Table 1, we can see that:

[0095] Comparative Examples 1 and 4 showed relatively obvious antioxidant effects, while Comparative Examples 2-3 and Comparative Examples 5-10 did not show obvious antioxidant effects. It is speculated that the black truffle extract and the white truffle extract can only exert their own certain antioxidant functions when used alone or in combination, and do not have a significant synergistic effect when combined with other extracts without antioxidant properties. After mixing with other extracts without antioxidant properties, the antioxidant properties of the composition may be unstable due to the reduced concentration of the extract with antioxidant properties or other mutual influences, and no particularly significant effect is produced.

[0096] In the technical scheme of the present invention, according to the results of Examples 1-8, the black truffle extract, the white truffle extract, the peach blossom extract and the yeast extract have a strong overall synergistic effect after being mixed, and show significant antioxidant effect; among them, compared with the effects of Comparative Examples 1-6, the antioxidant effect of Examples 7 and 8 is significantly decreased, so it can be seen that the compounding ratio of white truffle extract, black truffle extract, peach blossom extract and yeast extract will also have an important influence on the antioxidant effect of the composition.

[0097] The fluorescence test results of Examples 1-8, Comparative Example 1 and Comparative Example 4 were compared and analyzed among the groups to study the significant differences in the experiments. The results are shown in Table 2.

[0098] Table 2 Data comparison and analysis of the fluorescence test results of Examples 1-8 and Comparative Examples 1 and 4 (significant P value)

[0099] P-value Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Comparative Example 1 Comparative Example 4 Example 1 - 0.4593 0.2875 0.5888 0.4116 0.8580 0.0219 0.0364 0.0001 0.0004 Example 2 - - 0.1430 0.4678 0.9090 0.9732 0.0296 0.0098 0.0003 0.0002 Example 3 - - - 0.2979 0.8232 0.7462 0.0180 0.0248 0.0005 0.0002 Example 4 - - - - 0.8203 0.5045 0.0244 0.0071 0.0001 0.0014 Example 5 - - - - - 0.4920 0.0071 0.0203 0.00004 0.0002 Example 6 - - - - - - 0.0051 0.0288 0.0001 0.0004 Example 7 - - - - - - - 0.1048 0.0002 0.0155 Example 8 - - - - - - - - 0.0190 0.0096 Comparative Example 1 - - - - - - - - - 0.1549 Comparative Example 4 - - - - - - - - - -

[0100] According to the results in Table 2, we can see that:

[0101] There are significant differences between Examples 1-6 and Examples 7-8 (P < 0.05), indicating that the antioxidant effect of Examples 1-6 is more significant than that of Examples 7-8, indicating that the combination ratio of Examples 1-6 is better than that of Examples 7-8. There are significant differences (P < 0.05) between Comparative Examples 1 and Comparative Examples 4 and Examples 1-8, indicating that although Comparative Examples 1 and 4 can show certain antioxidant effects, they are not as significant as the effects of Examples 1-8.

[0102] 2. Skin care effect test of skin care products

[0103] 2.1 Skin care product ingredients and formula

[0104] Table 3 is the formula table of the purification solution of Example 1;

[0105] Table 3 Analysis of the formula of antioxidant test samples

[0106]

[0107] 2.2 Skin antioxidant effect test

[0108] 2.2.1 Antioxidant effect test of skin care products with different composition concentrations

[0109] Test Method

[0110] The sample concentration gradient gloss and smoothness test of Example 1 has a total of 9 groups of test objects (as shown in Table 3 test sample analysis), which are divided into 5 groups, and a total of 33*5 volunteers are recruited. It is necessary to ensure that there are 33 test objects in each group, and the final recovery data meets more than 30 people in each group.

[0111] The skin gloss and smoothness test of the essence containing 5% of the composition of Examples 1-8 and Comparative Examples 1-10 was conducted. A total of 18 groups of test subjects were divided into 9 groups in total, and a total of 33*9 volunteers were recruited. It was necessary to ensure that there were 33 test subjects in each group, and the final recovery data met the requirements of more than 30 people in each group.

[0112] Test instruments are shown in Table 4;

[0113] Table 4 Test instrument information

[0114] Instrument Name Model Specifications Skin gloss test probe Glosssymeter GL200 Skin Texture Imager Visioscan VC20plus

[0115] 1) Skin glossiness

[0116] Parameter description: The larger the measured value, the higher the skin glossiness

[0117] Result judgment: Before and after trial use of the product, the skin glossiness value increased significantly (P < 0.05), indicating that the test substance has the effect of improving skin glossiness.

[0118] Samples were prepared according to the essence formula in Table 3 as the test objects for multiple groups of skin gloss change tests, and the skin gloss results before use and after continuous use for 28 days were tested respectively. The results are shown in Table 5;

[0119] Table 5 Skin gloss test results of different concentrations of essence in Example 1

[0120] Group Day 0 Day 28 Rate of change P-value Significance Blank control 6.11 6.12 0.16％ ＞0.05 × Sample 1 5.97 6.11 2.35％ ＜0.05 √ Sample 2 5.93 6.15 3.71％ ＜0.05 √ Sample 3 6.08 6.43 5.76％ ＜0.001 √ Sample 4 6.13 6.66 8.65％ ＜0.001 √ Sample 5 5.99 6.59 10.02％ ＜0.001 √ Sample 6 6.01 6.74 12.15％ ＜0.001 √ Sample 7 6.07 6.83 12.52％ ＜0.001 √ Sample 8 5.93 6.66 12.31％ ＜0.001 √

[0121] According to the results in Table 5:

[0122] There was no significant difference in the skin gloss test data of the blank control after Day 0 and Day 28; there were significant differences in the skin gloss test data of sample 1 and sample 2 after Day 0 and Day 28, but the effect brought by the change rate was not high enough to meet the daily skin care needs of consumers; and there were extremely obvious significant differences in the skin gloss test data of samples 3-sample 8 after Day 0 and Day 28, but the difference in the change rate between sample 6 and sample 7 and sample 8 was not large, indicating that the content of the antioxidant function composition of the present invention in the skin care product is the most effective when it is 3-8%. It is best that when the content of the antioxidant composition of the present invention in the skin care product is higher than 8%, although it has significant antioxidant effect, the increase in concentration is of little significance. The above blank control and sample 1-sample 8 skin gloss test data results show that the blank control has little effect on skin antioxidant effect, 1.0-2.0% has antioxidant effect on the skin but the effect is not obvious, 3.0-80.0% concentration is more preferred for the composition, and as the concentration increases, the skin antioxidant effect is more significant; but when the concentration of the antioxidant composition of the present invention is greater than 8%, the effect has not been significantly improved.

[0123] 2) Skin smoothness SEsm

[0124] Parameter description: The smaller the measurement value, the smoother the skin

[0125] Result determination: Before and after trial use of the product, the skin smoothness value increased significantly (P < 0.05), indicating that the test substance has the effect of improving skin smoothness.

[0126] Samples were prepared according to the essence formula in Table 3 as test objects for multiple groups of skin glossiness change tests, and the skin smoothness before use and after continuous use for 28 days were tested respectively. The results are shown in Table 6;

[0127] Table 6 Skin smoothness test results of essences of different concentrations in Example 1

[0128] Group Day 0 Day 28 Rate of change P-value Significance Blank control 266.25 263.74 -0.94％ ＞0.05 × Sample 1 265.82 261.89 -1.48％ ＞0.05 × Sample 2 261.71 242.9 -7.19％ ＜0.05 √ Sample 3 260.09 234.71 -9.76％ ＜0.001 √ Sample 4 263.88 230.79 -12.54％ ＜0.001 √ Sample 5 259.69 219.32 -15.55％ ＜0.001 √ Sample 6 263.27 217.99 -17.20％ ＜0.001 √ Sample 7 264.02 216.81 -17.88％ ＜0.001 √ Sample 8 259.56 214.06 -17.53％ ＜0.001 √

[0129] According to the results in Table 6:

[0130] There was no significant difference in the skin smoothness test data of the blank control after Day 0 and Day 28; there were significant differences in the skin smoothness test data of sample 1 and sample 2 after Day 0 and Day 28, but the effect brought by the change rate was not high enough to meet the daily skin care needs of consumers; and there were extremely obvious significant differences in the skin smoothness test data of samples 3-sample 8 after Day 0 and Day 28, but the difference in the change rate between sample 6 and sample 7 and sample 8 was not large, indicating that the content of the antioxidant function composition of the present invention in the skin care product is the most effective when it is 3-8%. It is best that when the content of the antioxidant composition of the present invention in the skin care product is higher than 8%, although it has significant antioxidant effect, the increase in concentration is of little significance. The above blank control and sample 1-sample 8 skin smoothness test data results show that the blank control has little effect on skin antioxidant effect, 1.0-2.0% has antioxidant effect on the skin but the effect is not obvious, 3.0-80.0% concentration is more preferred for the composition, and with the increase of concentration, the skin antioxidant effect is more significant; but when the concentration of the antioxidant composition of the present invention is greater than 8%, the effect has not been significantly improved.

[0131] 2.2.2 Antioxidant effect test of skin care products containing the same concentration of the composition of Examples 1-8 and Comparative Examples 1-10 The test method is the same as the above-mentioned skin glossiness and skin smoothness SEsm test

[0132] According to the essence ratio of sample 4 in Table 3, 5% of the essences of the compositions of Examples 1-8 and Comparative Examples 1-10 were prepared respectively, and the skin glossiness effect and skin smoothness effect were tested respectively according to the above test method. The results are shown in Tables 7 and 8;

[0133] Table 7 Skin gloss test results of essence containing 5% of the composition of Examples 1-8 and Comparative Examples 1-10

[0134]

[0135]

[0136] According to the results in Table 7:

[0137] Examples 1-8 demonstrate that the present invention can effectively improve skin glossiness by compounding white truffle extract, black truffle extract, peach blossom extract and yeast extract. The main reason is that the compounding of the four extracts of the present invention produces a synergistic antioxidant effect, and the white truffle extract exerts its due anti-inflammatory effect, combined with the black truffle extract, peach blossom extract and yeast extract to promote the cell cycle repair function, which has a more significant effect on improving skin glossiness.

[0138] In contrast, in Comparative Examples 1-4, gentian extracts with similar functions were used to replace white truffle extracts, ginseng extracts to replace black truffle extracts, camellia extracts to replace peach blossom extracts, and bifid yeast lysates to replace yeast extracts, but the effects were not very satisfactory. This may be because the highly effective antioxidant function of the combination of white truffle extracts, black truffle extracts, peach blossom extracts and yeast extracts was lost, so the glossiness of the skin could not be significantly improved.

[0139] Comparative Examples 5 and 6 also use camellia extract and ginseng extract to replace the same amount of extract combination, which fully proves that the camellia extract and ginseng extract do not produce synergistic antioxidant effects not because the added weight is insufficient, but because they cannot produce synergistic effects when combined with other extracts, and cannot significantly improve the antioxidant effect of the composition;

[0140] In Comparative Examples 7-10, the white truffle extract, black truffle extract, peach blossom extract and yeast extract in the technical scheme of the present invention are removed one by one, and the composition with antioxidant function disclosed in the present invention is studied to determine which extract mainly produces antioxidant effect, so as to judge whether there are unimportant extracts in the compound formula. The results show that the lack of any one of the white truffle extract, black truffle extract, peach blossom extract and yeast extract in the technical scheme of the present invention will greatly reduce the antioxidant effect of the composition and produce no significant antioxidant effect. The present invention effectively improves the antioxidant function of the composition through the synergistic compounding of white truffle extract, black truffle extract, peach blossom extract and yeast extract.

[0141] Table 8 Skin smoothness test results of essence containing 5% of the composition of Examples 1-8 and Comparative Examples 1-10

[0142]

[0143]

[0144] According to the results in Table 8:

[0145] Examples 1-8 demonstrate that the present invention can effectively improve skin smoothness by compounding white truffle extract, black truffle extract, peach blossom extract and yeast extract. The main reason is that the compound has good free radical scavenging ability and antioxidant activity, which can accelerate the differentiation and proliferation rate of epidermal keratinocytes, and at the same time, by reducing inflammatory damage and promoting cell autophagy, further enhance the metabolism and immunity of the skin, improve skin microcirculation, and then promote the proliferation of collagen and elastic fibers, thereby achieving the effect of enhancing skin smoothness.

[0146] In contrast, in Comparative Examples 1-4, gentian extracts with similar functions were used to replace white truffle extracts, ginseng extracts to replace black truffle extracts, camellia extracts to replace peach blossom extracts, and bifid yeast lysates to replace yeast extracts, but the effects were not very satisfactory. This may be because the highly effective antioxidant function of the combination of white truffle extracts, black truffle extracts, peach blossom extracts and yeast extracts was lost, so the smoothness of the skin could not be significantly improved.

[0147] Comparative Examples 5 and 6 also use camellia extract and ginseng extract to replace the same amount of extract combination, which fully proves that the camellia extract and ginseng extract do not produce synergistic antioxidant effects not because the added weight is insufficient, but because they cannot produce synergistic effects when combined with other extracts, and cannot significantly improve the antioxidant effect of the composition;

[0148] In Comparative Examples 7-10, the white truffle extract, black truffle extract, peach blossom extract and yeast extract in the technical scheme of the present invention are removed one by one, and the composition with antioxidant function disclosed in the present invention is studied to determine which extract mainly produces antioxidant effect, so as to judge whether there are unimportant extracts in the compound formula. The results show that the lack of any one of the white truffle extract, black truffle extract, peach blossom extract and yeast extract in the technical scheme of the present invention will greatly reduce the antioxidant effect of the composition and produce no significant antioxidant effect. The present invention effectively improves the antioxidant function of the composition through the synergistic compounding of white truffle extract, black truffle extract, peach blossom extract and yeast extract.

[0149] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A composition with antioxidant function, characterized in that: The invention is composed of the following components by weight: 20-40% white truffle extract, 20-40% black truffle extract, 20-40% peach blossom extract and 20-40% yeast extract.

2. A skin care product, characterized in that: The invention comprises the composition with antioxidant function as claimed in claim 1, wherein the content of the composition with antioxidant function is ≥ 3%.

3. The skin care product according to claim 2, characterized in that: The content of the composition with antioxidant function is 3.0-8.0%.

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