Feed additive for treating horse gastric knot and preparation method thereof

Abstract

The invention discloses a feed additive for treating equine gastric stagnation and a preparation method thereof. The preparation method comprises the following steps: heating a herbal preparation, then cooling it to room temperature, adding a composite bacterial agent and molasses for fermentation under stirring, obtaining a fermentation liquid after the fermentation is completed, adding a modified carrier to the fermentation liquid and mixing evenly, then drying and crushing it by air blast at room temperature to obtain a composite agent; evenly mixing the composite agent, modified medicinal residues, mineral powder and glucose, drying and sieving to obtain a feed additive for treating equine gastric stagnation; the feed additive makes full use of the effective ingredients and medicinal residues of Chinese herbal medicines to strengthen the stomach and digestion, replenish the middle qi and eliminate gastrointestinal stagnation, and also provides a modified carrier to effectively prolong the action time and effect of the herbal preparation, and can adsorb and remove molds and toxins in the feed, and finally achieve a good therapeutic effect of equine gastric stagnation.

Description

Technical Field

[0001] The invention relates to the field of feed additives, and in particular to a feed additive for treating horse gastric knots and a preparation method thereof. Background Art

[0002] Gastric knot, also known as big belly knot, is a disease in which livestock overeats forage or too much gas accumulates in the stomach, causing the stomach wall to expand and causing abdominal pain. It is more common in horses and mules clinically. The disease is mainly caused by improper feeding and management, such as overeating indigestible forage when hungry, or stealing too much grain, especially overeating forage that is easy to swell, ferment, or rot; or due to sudden changes in feed, eating too much, and drinking water after being full, etc., all of which can cause forage to accumulate in the stomach and cannot be transported and transformed, causing this disease; or due to damage to yin and cold, qi stagnation, yang qi failure, gastric obstruction, and inability of water and grass to flow downward, causing this disease.

[0003] Chinese patent document CN105749172A discloses a drug for treating gastric calculi, which is composed of the following drugs: Herba Lysimachiae, Divine Comedy, Crataegus, Gallus Galli, Baical Skullcap Root, Fructus Aurantii Immaturus, Malt, Licorice, Rhubarb, Fried Gardenia Jasminoides, Peppermint, Curcuma, White Peony Root, Angelica Sinensis, Fructus Amomi, Taraxaci Herba, and the drug combination has the characteristics of dissolving stones, eliminating stones, expelling stones, digesting food, promoting qi, breaking up accumulation, promoting blood circulation and removing blood stasis, relieving pain, clearing away heat and detoxifying, and achieves the purpose of quickly curing gastric calculi and reducing the pain of patients. However, through searching patents, no drug specifically for treating horse stomach knot has been found.

[0004] Qian Zhichun (2018) proposed a method for treating stomach knot in horses in Comprehensive Prevention and Treatment Measures for Common Intestinal Diseases in Horses: acupuncture and moxibustion of acupoints such as Weishu, Sanjiang, Fenshui, Jiangya, and Hoof Head, combined with taking the Chinese medicine Tiaoqi Gonggong Powder; prescription: 40g of vinegar-scented cyperus, 40g of costusroot, 30g of patchouli, 40g of bitter orange, 40g of truncatum, 40g of green peel, 40g of three-edged lancea, 40g of curcuma, and 40g of radish seeds. Grind the above Chinese medicines into powder and take it with water or decoct it into a thick soup for oral administration. In practice, it was found that the effect was not good.

[0005] In addition, after the active ingredients are extracted from the production of traditional Chinese medicine, a large amount of discarded drug residues will be generated. Traditional treatment methods such as incineration, landfill or stacking will lead to resource waste and environmental pollution. Therefore, it is necessary to propose a feed additive that has good therapeutic effects and can simultaneously utilize the active ingredients and drug residues of traditional Chinese medicine. Summary of the invention

[0006] In order to solve the shortcomings of the prior art, the purpose of the present invention is to provide a feed additive for treating equine gastric stagnation, which fully utilizes the effective ingredients and residues of Chinese herbal medicines to play the roles of invigorating the stomach and digestion, replenishing the middle qi, and eliminating gastrointestinal stagnation. A modified carrier is also provided to effectively prolong the action time and effect of the herbal preparation, and can adsorb and remove mold and toxins in the feed, ultimately achieving a good therapeutic effect on equine gastric stagnation.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] A method for preparing a feed additive for treating equine gastric knots comprises the following steps:

[0009] (1) heating the herbal preparation, then cooling it to room temperature, adding the composite bacterial agent and molasses to ferment under stirring, obtaining a fermentation liquid after the fermentation is completed, adding the modified carrier to the fermentation liquid and mixing evenly, then drying and crushing it at room temperature to obtain a composite medicine;

[0010] (2) The compound agent, modified drug residue, mineral powder and glucose are mixed evenly, dried and sieved to obtain a feed additive for treating equine gastric knots.

[0011] Preferably, in step (1), the weight ratio of the herbal preparation, the composite bacterial agent, molasses, and the modified carrier is 150:15-30:25-50:40-60; in step (2), the weight ratio of the composite agent, the modified medicinal residue, the mineral powder, and glucose is 50:30-60:8-22:8-15.

[0012] Preferably, in step (1), the heating condition is heating to 70-90° C. and keeping warm for 10-20 min, the fermentation condition is anaerobic fermentation at 35-42° C. for 48-72 h, stirring every 1 h, and crushing to 40-80 mesh.

[0013] Preferably, in step (1), the composite bacterial agent is prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:1-2; in step (2), the mineral powder is prepared by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:2-3:1-2:1.5-3.

[0014] Preferably, the preparation method of the herbal preparation is as follows: weigh 40-60 parts of astragalus, 40-60 parts of walnut kernels, 30-50 parts of alpinia oxyphylla, 30-50 parts of ginger, 30-50 parts of elecampane, 30-50 parts of akebia, 30-40 parts of dandelion, 30-40 parts of rhubarb, 25-35 parts of green peel, 25-35 parts of hawthorn, 20-30 parts of Shenqu, 20-30 parts of radish seeds, and 15-25 parts of liquorice, dry the various Chinese herbal medicines at 55-70°C, crush them, blanch them for 20 minutes, and fry them for 15 minutes. ~40 mesh sieve, mix evenly to obtain a herbal composition; add 3 to 5 times the weight of water of the herbal composition, soak for 30 to 60 minutes, boil over high heat, keep warm over low heat for 20 to 30 minutes, filter out the medicinal liquid, then add 3 to 5 times the weight of water of the herbal composition, boil over high heat, keep warm over low heat for 30 to 40 minutes, filter out the medicinal liquid, then add 3 to 5 times the weight of water of the herbal composition, boil over high heat, keep warm over low heat for 40 to 50 minutes, filter out the medicinal liquid, keep the residue for later use, and combine the three medicinal liquids to obtain a herbal preparation.

[0015] Preferably, the preparation method of the modified medicinal residue is as follows: soak the medicinal residue in a 3-6wt% NaCl solution for 0.5-1.5h, filter, dry, crush, pass through a 40-mesh sieve, and then pyrolyze at 300-400°C for 2-3h, cool to room temperature, mix evenly with a 10-15wt% phosphoric acid solution, and react at 100-120°C for 60-90min to obtain the modified medicinal residue.

[0016] Preferably, the preparation method of the modified carrier is as follows: activate the carrier at 300-400°C for 20-40min, add it to a 50-70wt% ethanol solution after cooling to room temperature, stir and react at 50-70°C for 2-3h, then cool to room temperature, add an acidulant dropwise, stir and react for 3-4h, filter, dry and sieve to obtain the modified carrier.

[0017] Preferably, the carrier is a mixture of bentonite and rectorite in a weight ratio of 1:1-2; the acidulant is a mixed solution of 4-10wt% lactic acid and 15-20wt% citric acid; the mass volume ratio of the carrier to the ethanol solution is 1g:10-20mL; the mass volume ratio of the carrier to the acidulant is 1g:2-6mL.

[0018] The present invention also claims protection for a feed additive prepared by the preparation method.

[0019] Preferably, the feed additive is added in an amount of 0.4-1.4% in the horse basal diet.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1) The present invention provides a feed additive for treating equine gastric stagnation. The herbal preparation plays the role of removing the old and the old, strengthening the middle palace, moistening dryness and relieving constipation, relaxing the stomach and regulating qi, and promoting qi and relieving pain, and can effectively treat equine gastric stagnation; astragalus, walnut kernel, alpinia oxyphylla, and ginger nourish the middle qi and warm the spleen yang; inulin strengthens the spleen and stomach, regulates qi and relieves depression; akebia and dandelion clear away heat and detoxify, promote diuresis and relieve stranguria, clear the heart and relieve restlessness; rhubarb has the function of purgation, clearing away heat and purging fire; citrus aurantium and hawthorn nourish yin and clear heat, promote fluid and quench thirst; Shenqu and radish seeds help digestion and relieve bloating, promote fluid and nourish the stomach, and increase fluid to promote movement; liquorice nourishes the spleen and qi and harmonizes various medicines.

[0022] 2) The present invention provides a feed additive for treating equine gastric stagnation. The carrier is modified by high-temperature activation and ethanol and acidifier, thereby effectively improving the pore structure in the carrier and enhancing its loading capacity. The modified carrier is used to load the herbal preparation, which not only enables the herbal medicine to slowly disintegrate and gradually release in the gastrointestinal tract of the horse, effectively increasing the drug action time, but also alleviates the irritation of the drug, reduces toxicity and adverse reactions; the herbal preparation is fermented by a composite bacterial agent, so that the glycosides, sugars and other effective ingredients in the herbal medicine are more easily absorbed by the horse, and at the same time, the composite bacterial agent is carried into the horse's stomach through the modified carrier, which improves the intestinal flora of the horse and regulates the horse's gastrointestinal digestion ability.

[0023] 3) The present invention provides a feed additive for treating equine gastric stagnation. The drug residue is carbonized by high-temperature pyrolysis to remove the bad smell of traditional Chinese medicine and benefit the horses' feeding. The carbonized drug residue is modified by phosphoric acid to add functional groups on the surface of the carbonized drug residue, so that it can effectively adsorb harmful substances such as mold and toxins in the feed, thereby reducing the incidence of equine gastric stagnation. At the same time, the modified drug residue also plays a role in regulating the gastrointestinal function of the horse, can inhibit the growth of harmful bacteria and pathogens, and play a role in preventing the onset of equine gastric stagnation.

[0024] 4) The present invention provides a feed additive for treating equine gastric stagnation. Among the mineral powders, talc, potassium saltpeter and gypsum play the role of clearing gastrointestinal stagnation, reducing turbidity and promoting bowel movements; aluminum hydroxide plays the role of neutralizing excess gastric acid, treating nausea and vomiting, loss of appetite and indigestion; talc, potassium saltpeter, gypsum and aluminum hydroxide synergistically play the role of strengthening the stomach and promoting digestion, reducing turbidity and promoting bowel movements. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with the embodiments. Of course, the specific embodiments described here are only used to explain the present invention and are not used to limit the present invention.

[0026] Unless otherwise specified, the chemical reagents and materials in the present invention are purchased from commercial sources or synthesized from raw materials purchased from commercial sources.

[0027] Bentonite was purchased from Lingshou County Tuolin Mineral Products Processing Plant, mesh size 200;

[0028] Rectorite was purchased from Lingshou County Deheng Mineral Products Processing Plant, mesh size 200;

[0029] Lactobacillus acidophilus powder was purchased from Shandong Jiaoyang Biotechnology Co., Ltd., with a content of 10 billion CFU / g;

[0030] Yeast was purchased from Shandong Nuojie Biotechnology Co., Ltd., with a content of 20 billion CFU / g;

[0031] All Chinese medicines were purchased from Sinopharm Pharmacy.

[0032] Example 1

[0033] A method for preparing a feed additive for treating equine gastric knots comprises the following steps:

[0034] (1) Weigh 60 g of astragalus, 60 g of walnut kernel, 50 g of alpinia oxyphylla, 50 g of ginger, 50 g of elecampane, 50 g of akebia, 40 g of dandelion, 40 g of rhubarb, 35 g of green tangerine peel, 35 g of hawthorn, 30 g of Shenqu, 30 g of radish seed, and 25 g of liquorice according to weight, dry each Chinese herbal medicine at 70° C., grind, pass through a 40-mesh sieve, and mix well to obtain a herbal composition;

[0035] (2) adding the herbal composition obtained in step (1) to 2500 g of water, soaking for 60 min, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 50 min, filtering out the medicinal liquid, obtaining a medicinal residue, and combining the three medicinal liquids to obtain a herbal preparation;

[0036] (3) Soaking the drug residue in a 6 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 400 ° C for 3 h, cooling to room temperature, mixing evenly with a 15 wt % phosphoric acid solution, and reacting at 120 ° C for 90 min to obtain a modified drug residue;

[0037] (4) 20 g of bentonite and 40 g of rectorite were mixed uniformly to obtain a carrier, activated at 400° C. for 30 min, cooled to room temperature, added to 1200 mL of 60 wt% ethanol solution, stirred at 70° C. and 100 r / min for 3 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 360 mL of 10 wt% lactic acid and 20 wt% citric acid, stirred at 150 r / min for 4 h, filtered, dried, and sieved to obtain a modified carrier;

[0038] (5) 150 g of the herbal preparation was heated to 80° C. for 20 min, cooled to room temperature, and 50 g of molasses was added under stirring, followed by 30 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:2. The mixture was anaerobically fermented at 42° C. for 72 h, stirred every 1 h, and a fermentation liquid was obtained after the fermentation was completed. 60 g of the modified carrier was added to the fermentation liquid and mixed evenly, and then dried and crushed at room temperature, and passed through an 80-mesh sieve to obtain a composite agent.

[0039] (6) 50 g of the compound drug is mixed evenly with 60 g of the modified drug residue and 15 g of glucose, and then 22 g of mineral powder obtained by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:3:2:3 is added. After stirring evenly, the mixture is dried and passed through an 80-mesh sieve to obtain a feed additive for treating equine gastric congestion.

[0040] The amount of feed additive added to the horse basic feed is 1.4%.

[0041] Example 2

[0042] A method for preparing a feed additive for treating equine gastric knots comprises the following steps:

[0043] (1) Weigh 40 g of astragalus, 40 g of walnut kernel, 30 g of alpinia oxyphylla, 30 g of ginger, 30 g of elecampane, 30 g of akebia, 30 g of dandelion, 30 g of rhubarb, 25 g of green tangerine peel, 25 g of hawthorn, 20 g of Shenqu, 20 g of radish seed, and 15 g of liquorice, dry each Chinese herbal medicine at 55° C., grind it, pass it through a 20-mesh sieve, and mix it evenly to obtain a herbal composition;

[0044] (2) adding the herbal composition obtained in step (1) to 1500 g of water, soaking for 30 min, boiling over high heat, keeping warm over low heat for 20 min, filtering out the medicinal liquid, adding another 1500 g of water, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 1500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, obtaining a medicinal residue, and combining the three medicinal liquids to obtain a herbal preparation;

[0045] (3) Soaking the drug residue in a 3 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 300 ° C for 2 h, cooling to room temperature, mixing evenly with a 10 wt % phosphoric acid solution, and reacting at 100 ° C for 60 min to obtain a modified drug residue;

[0046] (4) 30 g of bentonite and 30 g of rectorite were uniformly mixed to obtain a carrier, activated at 300° C. for 30 min, cooled to room temperature, added to 600 mL of 60 wt% ethanol solution, stirred at 50° C. and 100 r / min for 2 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 120 mL of 4 wt% lactic acid and 15 wt% citric acid, stirred at 150 r / min for 3 h, filtered, dried, and sieved to obtain a modified carrier;

[0047] (5) heating 150 g of the herbal preparation to 80° C. for 10 min, cooling to room temperature, adding 25 g of molasses while stirring, and then adding 15 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:1, and anaerobically fermenting at 35° C. for 48 h, stirring every 1 h, and obtaining a fermentation liquid after the fermentation is completed, and then adding 40 g of the modified carrier to the fermentation liquid and mixing evenly, and then drying and crushing at room temperature, and passing through a 40-mesh sieve to obtain a composite agent;

[0048] (6) 50 g of the compound agent is mixed evenly with 30 g of the modified drug residue and 8 g of glucose, and then 8 g of mineral powder obtained by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:2:1:1.5 is added. After stirring evenly, the mixture is dried and passed through an 80-mesh sieve to obtain a feed additive for treating equine gastric congestion.

[0049] The amount of feed additive added to the horse basic feed is 0.4%.

[0050] Example 3

[0051] A method for preparing a feed additive for treating equine gastric knots comprises the following steps:

[0052] (1) Weigh 50 g of astragalus, 50 g of walnut kernel, 40 g of alpinia oxyphylla, 40 g of ginger, 40 g of elecampane, 40 g of akebia, 35 g of dandelion, 35 g of rhubarb, 30 g of green tangerine peel, 30 g of hawthorn, 25 g of Shenqu, 25 g of radish seed, and 20 g of liquorice, dry each Chinese herbal medicine at 60° C., grind it, pass it through a 40-mesh sieve, and mix it evenly to obtain a herbal composition;

[0053] (2) adding the herbal composition obtained in step (1) to 2000 g of water, soaking for 50 min, boiling over high heat, keeping warm over low heat for 25 min, filtering out the medicinal liquid, adding 2000 g of water again, boiling over high heat, keeping warm over low heat for 35 min, filtering out the medicinal liquid, adding 2000 g of water again, boiling over high heat, keeping warm over low heat for 45 min, filtering out the medicinal liquid, obtaining medicinal residues, and combining the three medicinal liquids to obtain a herbal preparation;

[0054] (3) Soaking the drug residue in a 5 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 350° C. for 3 h. After cooling to room temperature, mixing evenly with a 12 wt % phosphoric acid solution, and reacting at 110° C. for 80 min, a modified drug residue was obtained;

[0055] (4) 24 g of bentonite and 36 g of rectorite were mixed to obtain a carrier, activated at 350° C. for 30 min, cooled to room temperature, added to 900 mL of 60 wt% ethanol solution, stirred at 60° C. and 100 r / min for 2 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 240 mL of 8 wt% lactic acid and 18 wt% citric acid, stirred at 150 r / min for 3 h, filtered, dried, and sieved to obtain a modified carrier;

[0056] (5) 150 g of the herbal preparation was heated to 80° C. for 15 min, cooled to room temperature, 40 g of molasses was added under stirring, and then 20 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:1.5 was added, and anaerobically fermented at 38° C. for 60 h, with stirring every 1 h. After the fermentation was completed, a fermentation liquid was obtained, 50 g of the modified carrier was added to the fermentation liquid and mixed evenly, and then dried with air at room temperature, crushed, and passed through a 60-mesh sieve to obtain a composite agent;

[0057] (6) 50 g of the compound drug is mixed evenly with 50 g of the modified drug residue and 10 g of glucose, and then 18 g of mineral powder obtained by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:2:1:1.5 is added. After stirring evenly, the mixture is dried and passed through an 80-mesh sieve to obtain a feed additive for treating equine gastric congestion.

[0058] The feed additive is added in the basic horse diet at a rate of 1%.

[0059] Comparative Example 1

[0060] A method for preparing a feed additive comprises the following steps:

[0061] (1) Weigh 60 g of astragalus, 60 g of walnut kernel, 50 g of alpinia oxyphylla, 50 g of ginger, 50 g of elecampane, 50 g of akebia, 40 g of dandelion, 40 g of rhubarb, 35 g of green tangerine peel, 35 g of hawthorn, 30 g of Shenqu, 30 g of radish seed, and 25 g of liquorice according to weight, dry each Chinese herbal medicine at 70° C., grind, pass through a 40-mesh sieve, and mix well to obtain a herbal composition;

[0062] (2) adding the herbal composition obtained in step (1) to 2500 g of water, soaking for 60 min, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 50 min, filtering out the medicinal liquid, and obtaining medicinal residues;

[0063] (3) Soaking the drug residue in a 6 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 400 ° C for 3 h, cooling to room temperature, mixing evenly with a 15 wt % phosphoric acid solution, and reacting at 120 ° C for 90 min to obtain a modified drug residue;

[0064] (4) 20 g of bentonite and 40 g of rectorite were mixed uniformly to obtain a carrier, activated at 400° C. for 30 min, cooled to room temperature, added to 1200 mL of 60 wt% ethanol solution, stirred at 70° C. and 100 r / min for 3 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 360 mL of 10 wt% lactic acid and 20 wt% citric acid, stirred at 150 r / min for 4 h, filtered, dried, and sieved to obtain a modified carrier;

[0065] (5) 150 g of distilled water was heated to 80° C. and kept warm for 20 min, then cooled to room temperature, and 50 g of molasses and 30 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:2 were added under stirring, and anaerobically fermented at 42° C. for 72 h, with stirring every 1 h. After the fermentation was completed, a fermentation liquid was obtained, and then 60 g of the modified carrier was added to the fermentation liquid and mixed evenly, and then dried and crushed at room temperature, and passed through an 80-mesh sieve to obtain a composite carrier;

[0066] (6) 50 g of the composite carrier was mixed evenly with 60 g of the modified drug residue and 15 g of glucose, and then 22 g of mineral powder obtained by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:3:2:3 was added. After stirring evenly, the mixture was dried and passed through an 80-mesh sieve to obtain a feed additive.

[0067] The amount of feed additive added to the horse basic feed is 1.4%.

[0068] Comparative Example 2

[0069] A method for preparing a feed additive comprises the following steps:

[0070] (1) Weigh 60 g of astragalus, 60 g of walnut kernel, 50 g of alpinia oxyphylla, 50 g of ginger, 50 g of elecampane, 50 g of akebia, 40 g of dandelion, 40 g of rhubarb, 35 g of green tangerine peel, 35 g of hawthorn, 30 g of Shenqu, 30 g of radish seed, and 25 g of liquorice according to weight, dry each Chinese herbal medicine at 70° C., grind, pass through a 40-mesh sieve, and mix well to obtain a herbal composition;

[0071] (2) adding the herbal composition obtained in step (1) to 2500 g of water, soaking for 60 min, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 50 min, filtering out the medicinal liquid, obtaining a medicinal residue, and combining the three medicinal liquids to obtain a herbal preparation;

[0072] (3) 20 g of bentonite and 40 g of rectorite were mixed uniformly to obtain a carrier, activated at 400° C. for 30 min, cooled to room temperature, added to 1200 mL of 60 wt% ethanol solution, stirred at 70° C. and 100 r / min for 3 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 360 mL of 10 wt% lactic acid and 20 wt% citric acid, stirred at 150 r / min for 4 h, filtered, dried, and sieved to obtain a modified carrier;

[0073] (4) heating 150 g of the herbal preparation to 80° C. for 20 min, cooling to room temperature, adding 50 g of molasses while stirring, and then adding 30 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:2, and anaerobically fermenting at 42° C. for 72 h, stirring every 1 h, and obtaining a fermentation liquid after the fermentation is completed. Adding 60 g of the modified carrier to the fermentation liquid and mixing evenly, and then drying and crushing at room temperature, and passing through an 80-mesh sieve to obtain a composite agent;

[0074] (5) 50 g of the composite drug, 60 g of the residue obtained in step (2), and 15 g of glucose are mixed evenly, and then 22 g of mineral powder obtained by mixing talc, saltpeter, gypsum, and aluminum hydroxide in a weight ratio of 1:3:2:3 is added. After stirring evenly, the mixture is dried and passed through an 80-mesh sieve to obtain a feed additive.

[0075] The amount of feed additive added to the horse basic feed is 1.4%.

[0076] Comparative Example 3

[0077] A method for preparing a feed additive comprises the following steps:

[0078] (1) Weigh 60 g of astragalus, 60 g of walnut kernel, 50 g of alpinia oxyphylla, 50 g of ginger, 50 g of elecampane, 50 g of akebia, 40 g of dandelion, 40 g of rhubarb, 35 g of green tangerine peel, 35 g of hawthorn, 30 g of Shenqu, 30 g of radish seed, and 25 g of liquorice according to weight, dry each Chinese herbal medicine at 70° C., grind, pass through a 40-mesh sieve, and mix well to obtain a herbal composition;

[0079] (2) adding the herbal composition obtained in step (1) to 2500 g of water, soaking for 60 min, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 50 min, filtering out the medicinal liquid, obtaining a medicinal residue, and combining the three medicinal liquids to obtain a herbal preparation;

[0080] (3) Soaking the drug residue in a 6 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 400 ° C for 3 h, cooling to room temperature, mixing evenly with a 15 wt % phosphoric acid solution, and reacting at 120 ° C for 90 min to obtain a modified drug residue;

[0081] (4) heating 150 g of the herbal preparation to 80° C. for 20 min, cooling to room temperature, adding 50 g of molasses while stirring, and then adding 30 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:2, and anaerobically fermenting at 42° C. for 72 h, stirring every 1 h, and obtaining a fermentation liquid after the fermentation is completed. Adding a carrier obtained by mixing 20 g of bentonite and 40 g of rectorite to the fermentation liquid and mixing evenly, and then drying and crushing at room temperature, and passing through an 80-mesh sieve to obtain a composite agent;

[0082] (5) 50 g of the composite agent was mixed evenly with 60 g of the modified drug residue and 15 g of glucose, and then 22 g of mineral powder obtained by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:3:2:3 was added, and the mixture was stirred evenly, dried and passed through an 80-mesh sieve to obtain a feed additive.

[0083] The amount of feed additive added to the horse basic feed is 1.4%.

[0084] Comparative Example 4

[0085] A method for preparing a feed additive comprises the following steps:

[0086] (1) Weigh 60 g of astragalus, 60 g of walnut kernel, 50 g of alpinia oxyphylla, 50 g of ginger, 50 g of elecampane, 50 g of akebia, 40 g of dandelion, 40 g of rhubarb, 35 g of green tangerine peel, 35 g of hawthorn, 30 g of Shenqu, 30 g of radish seed, and 25 g of liquorice according to weight, dry each Chinese herbal medicine at 70° C., grind, pass through a 40-mesh sieve, and mix well to obtain a herbal composition;

[0087] (2) adding the herbal composition obtained in step (1) to 2500 g of water, soaking for 60 min, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 50 min, filtering out the medicinal liquid, obtaining a medicinal residue, and combining the three medicinal liquids to obtain a herbal preparation;

[0088] (3) Soaking the drug residue in a 6 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 400 ° C for 3 h, cooling to room temperature, mixing evenly with a 15 wt % phosphoric acid solution, and reacting at 120 ° C for 90 min to obtain a modified drug residue;

[0089] (4) 20 g of bentonite and 40 g of rectorite were mixed uniformly to obtain a carrier, activated at 400° C. for 30 min, cooled to room temperature, added to 1200 mL of 60 wt% ethanol solution, stirred at 70° C. and 100 r / min for 3 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 360 mL of 10 wt% lactic acid and 20 wt% citric acid, stirred at 150 r / min for 4 h, filtered, dried, and sieved to obtain a modified carrier;

[0090] (5) 150 g of the herbal preparation was heated to 80° C. for 20 min, cooled to room temperature, and 50 g of molasses and 60 g of the modified carrier were added under stirring. After mixing evenly, the mixture was dried by air blowing at room temperature, crushed, and passed through an 80-mesh sieve to obtain a composite medicine;

[0091] (6) 50 g of the composite agent was mixed evenly with 60 g of the modified drug residue and 15 g of glucose, and then 22 g of mineral powder obtained by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:3:2:3 was added. After stirring evenly, the mixture was dried and passed through an 80-mesh sieve to obtain a feed additive.

[0092] The amount of feed additive added to the horse basic feed is 1.4%.

[0093] Comparative Example 5

[0094] A method for preparing a feed additive comprises the following steps:

[0095] (1) Weigh 60 g of astragalus, 60 g of walnut kernel, 50 g of alpinia oxyphylla, 50 g of ginger, 50 g of elecampane, 50 g of akebia, 40 g of dandelion, 40 g of rhubarb, 35 g of green tangerine peel, 35 g of hawthorn, 30 g of Shenqu, 30 g of radish seed, and 25 g of liquorice according to weight, dry each Chinese herbal medicine at 70° C., grind, pass through a 40-mesh sieve, and mix well to obtain a herbal composition;

[0096] (2) adding the herbal composition obtained in step (1) to 2500 g of water, soaking for 60 min, boiling over high heat, keeping warm over low heat for 30 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 40 min, filtering out the medicinal liquid, adding another 2500 g of water, boiling over high heat, keeping warm over low heat for 50 min, filtering out the medicinal liquid, obtaining a medicinal residue, and combining the three medicinal liquids to obtain a herbal preparation;

[0097] (3) Soaking the drug residue in a 6 wt % NaCl solution for 1 h, filtering, drying, crushing, passing through a 40-mesh sieve, and then pyrolyzing at 400 ° C for 3 h, cooling to room temperature, mixing evenly with a 15 wt % phosphoric acid solution, and reacting at 120 ° C for 90 min to obtain a modified drug residue;

[0098] (4) 20 g of bentonite and 40 g of rectorite were mixed uniformly to obtain a carrier, activated at 400° C. for 30 min, cooled to room temperature, added to 1200 mL of 60 wt% ethanol solution, stirred at 70° C. and 100 r / min for 3 h, then cooled to room temperature, added dropwise to an acidulant obtained by mixing 360 mL of 10 wt% lactic acid and 20 wt% citric acid, stirred at 150 r / min for 4 h, filtered, dried, and sieved to obtain a modified carrier;

[0099] (5) 150 g of the herbal preparation was heated to 80° C. for 20 min, cooled to room temperature, and 50 g of molasses was added under stirring, followed by 30 g of a composite bacterial agent prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:2. The mixture was anaerobically fermented at 42° C. for 72 h, stirred every 1 h, and a fermentation liquid was obtained after the fermentation was completed. 60 g of the modified carrier was added to the fermentation liquid and mixed evenly, and then dried and crushed at room temperature, and passed through an 80-mesh sieve to obtain a composite agent.

[0100] (6) 50 g of the composite agent, 60 g of the modified drug residue, and 15 g of glucose were mixed evenly, dried, and passed through an 80-mesh sieve to obtain a feed additive.

[0101] The amount of feed additive added to the horse basic feed is 1.4%.

[0102] 1. Treatment methods

[0103] 80 horses suffering from gastric knots were selected and divided into 8 groups on average, with 7 stallions and 3 mares in each group. The feed additives prepared in Examples 1 to 3 and Comparative Examples 1 to 5 were added to the horse feed according to the addition ratio, once a day, every morning, for a course of 5 days.

[0104] 2. Observation Project

[0105] The sick horse has abdominal pain and is restless, walks quickly with its head down, and some sit in a dog-like posture. It breathes rapidly, often belches with a sour and smelly odor, often vomits, and even vomits feces. The mouth is red, the tongue is greasy, and the pulse is stringy and slippery. After treatment, observe for 2 months to see if there is a recurrence.

[0106] 3. Efficacy Standards

[0107] Recovery: clinical symptoms disappear, there is no recurrence after the high-incidence season, and laboratory tests are normal;

[0108] Improvement: clinical symptoms are alleviated, the number of attacks decreases during the high-risk season, and laboratory tests improve or become normal;

[0109] Ineffective: Clinical symptoms did not improve or worsen significantly.

[0110] IV. Treatment Outcomes

[0111] In the feed additives prepared by each group, no herbal preparation was added in the comparative example 1 group, the carrier was not modified in the comparative example 3 group, the herbal preparation was not fermented in the comparative example 4 group, and mineral powder was not added in the comparative example 5 group, all of which had a certain influence on the therapeutic effect of the prepared feed additives. The specific data are shown in Table 1.

[0112] Table 1 Comparison of the therapeutic effects of Examples 1 to 3 and Comparative Examples 1 to 5

[0113]

[0114]

[0115] On the 0th and 5th days of the experiment, horse feces samples were collected 1 hour after the end of feeding with feed additives for the cultivation of Escherichia coli and Lactobacillus.

[0116] The method for counting the number of E. coli colonies is as follows: fecal samples were collected from the rectum of each group of horses (about 25 cm from the anus), immediately placed in a sterile beaker preheated to 39°C, 0.1 mL of fecal samples were diluted, inoculated into eosin-methylene blue agar medium, and placed in a constant temperature culture at 37°C for 24 hours for E. coli counting. The method for counting lactobacilli colonies was the same as the E. coli colony technique method, the culture medium was replaced with LBS selective medium, and the pH was adjusted to 5.5 with glacial acetic acid.

[0117] The feed additive prepared in Comparative Example 2 did not modify the medicinal residue, and the feed additive prepared in Comparative Example 4 did not ferment the herbal preparation using a composite bacterial agent, so the two groups of feed additives had no significant inhibitory effects on harmful bacteria and promotion of beneficial bacteria. Specific data are shown in Table 2.

[0118] Table 2 Effects of feed additives on harmful and beneficial bacteria (CFU / mL)

[0119]

[0120] The feed additives prepared in Example 1 and Comparative Example 2 were tested for the detoxification effect of mycotoxins. The mycotoxins were aflatoxin B1, fumonisin, T2 toxin, and gibberellin. A certain amount of the above-mentioned mycotoxin standard solution (specific toxin content is shown in Table 3) was taken respectively, and then the feed additives prepared in Example 1 and Comparative Example 2 were added to make the concentration of the feed additive sample in the solution 3 mg / mL. After adding the feed additive to the mycotoxin standard solution, the sample was adsorbed at a constant temperature of 37°C and 150r / min for 2h, and then the supernatant was collected by centrifugation, the content of each mycotoxin in the supernatant was determined, and the mycotoxin adsorption amount and adsorption rate were calculated.

[0121] Compared with Example 1, the feed additive prepared in Comparative Example 2 did not modify the drug residue, so its adsorption effect on various feed mycotoxins was significantly worse than that of the feed additive prepared in Example 1. Specific data are shown in Table 3.

[0122] Table 3 Detoxification effect of feed additives prepared in Example 1 and Comparative Example 2

[0123]

[0124] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for preparing a feed additive for treating equine gastric knot, characterized in that: The following steps are involved: (1) The herbal preparation is heated, then cooled to room temperature, and a composite bacterial agent and molasses are added under stirring for fermentation. After the fermentation is completed, a fermentation liquid is obtained, and the modified carrier is added to the fermentation liquid and mixed evenly, and then the mixture is dried and crushed at room temperature to obtain a composite medicine; (2) Evenly mixing the compound medicine, modified medicine residue, mineral powder and glucose, drying and sieving to obtain a feed additive for treating equine gastric knot; The preparation method of the herbal medicine preparation is as follows: weigh 40-60 parts of astragalus, 40-60 parts of walnut kernels, 30-50 parts of alpinia oxyphylla, 30-50 parts of ginger, 30-50 parts of elecampane, 30-50 parts of akebia, 30-40 parts of dandelion, 30-40 parts of rhubarb, 25-35 parts of green peel, 25-35 parts of hawthorn, 20-30 parts of Shenqu, 20-30 parts of radish seeds, and 15-25 parts of liquorice, dry the various Chinese herbal medicines at 55-70°C, crush them, and blanch them for 20-4 0 mesh sieve, mix evenly to obtain a herbal composition; add 3 to 5 times the weight of water of the herbal composition, soak for 30 to 60 minutes, boil over high heat, keep warm over low heat for 20 to 30 minutes, filter out the medicinal liquid, then add 3 to 5 times the weight of water of the herbal composition, boil over high heat, keep warm over low heat for 30 to 40 minutes, filter out the medicinal liquid, then add 3 to 5 times the weight of water of the herbal composition, boil over high heat, keep warm over low heat for 40 to 50 minutes, filter out the medicinal liquid, and set aside the residue; combine the three medicinal liquids to obtain a herbal preparation; The preparation method of the modified drug residue is as follows: soak the drug residue in a 3-6wt% NaCl solution for 0.5-1.5h, filter, dry, crush, pass through a 40-mesh sieve, and then pyrolyze at 300-400°C for 2-3h, cool to room temperature, mix evenly with a 10-15wt% phosphoric acid solution, and react at 100-120°C for 60-90min to obtain the modified drug residue; The preparation method of the modified carrier is as follows: activating the carrier at 300-400° C. for 20-40 minutes, cooling to room temperature, adding the carrier to a 50-70wt% ethanol solution, stirring and reacting at 50-70° C. for 2-3 hours, then cooling to room temperature, dropping an acidifier, stirring and reacting for 3-4 hours, filtering, drying, and sieving to obtain the modified carrier; The carrier is prepared by uniformly mixing bentonite and rectorite in a weight ratio of 1:1-2; the acidulant is a mixed solution of 4-10wt% lactic acid and 15-20wt% citric acid; the mass volume ratio of the carrier to the ethanol solution is 1g:10-20mL; and the mass volume ratio of the carrier to the acidulant is 1g:2-6mL.

2. The preparation method according to claim 1, characterized in that: In step (1), the weight ratio of the herbal preparation, the composite bacterial agent, molasses, and the modified carrier is 150:15~30:25~50:40~60; in step (2), the weight ratio of the composite agent, the modified medicinal residue, the mineral powder, and glucose is 50:30~60:8~22:8~15.

3. The preparation method according to claim 1, characterized in that: In step (1), the heating condition is to heat to 70-90°C and keep warm for 10-20 minutes, the fermentation condition is to perform anaerobic fermentation at 35-42°C for 48-72 hours, stir every hour, and crush into 40-80 mesh.

4. The preparation method according to claim 1, characterized in that: In step (1), the composite bacterial agent is prepared by mixing Lactobacillus acidophilus and yeast in a weight ratio of 2:1-2; in step (2), the mineral powder is prepared by mixing talc, saltpeter, gypsum and aluminum hydroxide in a weight ratio of 1:2-3:1-2:1.5-3.

5. A feed additive prepared by the preparation method according to any one of claims 1 to 4.

6. The feed additive according to claim 5, characterized in that The feed additive is added in an amount of 0.4-1.4% in the basic horse diet.

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