Self-assembled biogels and methods of making and using the same

Abstract

The application provides a kind of self-assembly based biological gel and its preparation method and application.The biological gel comprises palmitoyl pentapeptide-4, polydeoxyribonucleotide and chitosan, wherein the polydeoxyribonucleotide is encapsulated in the self-assembly structure of palmitoyl pentapeptide-4, the chitosan is coated outside the self-assembly structure by electrostatic interaction, and the mass ratio of palmitoyl pentapeptide-4 to polydeoxyribonucleotide is 1.5-30:1.The combination of palmitoyl pentapeptide-4, PDRN and chitosan improves the bioavailability and transdermal absorption of active substances and produces synergistic anti-aging effect, greatly improving the anti-aging effect.

Description

Technical Field

[0001] This application relates to the field of biotechnology, specifically to self-assembled biogels, their preparation methods, and applications. Background Technology

[0002] Any discussion of prior art throughout the specification should not be construed as an admission that such prior art is well-known or constitutes part of common general knowledge in the art.

[0003] The main characteristics of skin aging are sagging, dullness, increased wrinkles, reduced smoothness, and the appearance of pigmentation spots. However, aging is no longer the sole cause of skin aging. Daily air pollution and ultraviolet radiation can cause irreversible damage to skin cells, leading to premature aging characteristics such as clogged pores, dullness, and age spots. Therefore, anti-aging is an essential demand for consumers in their daily skincare routine, and cosmetics with anti-aging effects are emerging in large numbers.

[0004] Peptides are composed of amino acids and are intermediate products of protein hydrolysis. As a natural active ingredient, peptides can promote cell growth and repair. Their wrinkle-reducing and anti-aging effects have been verified by research and are an important component of anti-aging cosmetics.

[0005] Self-assembled peptide nanogels have wide applications in tissue engineering and drug delivery. Their nanoscale size enhances transdermal permeability, while good biocompatibility ensures safety during use. However, in the cosmetics field, research on using self-assembled peptides to improve transdermal permeability is scarce.

[0006] The object of this invention is to overcome or improve at least one disadvantage of the prior art, or to provide a useful alternative. Unless the context clearly requires otherwise, the words “comprising” and “including” should be interpreted in an inclusive sense rather than an exclusive or exhaustive sense throughout the specification and claims; that is, in the sense of “including, but not limited to”. Summary of the Invention

[0007] This invention provides a self-assembled biogel composition, its preparation method, and its applications. The biogel composition of this invention exhibits good stability, safety, and biocompatibility; it is non-irritating and non-sensitizing to the skin; it significantly enhances anti-aging effects, particularly improving rough skin and reducing fine lines; and it is fast-acting and highly effective.

[0008] Specifically, the technical solution of the present invention is as follows:

[0009] In a first aspect, the present invention provides a biogel composition comprising palmitoyl pentapeptide-4, polydeoxyribonucleotide (PDRN), and chitosan, wherein PDRN is loaded in a self-assembled structure of palmitoyl pentapeptide-4, and chitosan is coated on the outside of the self-assembled structure by electrostatic interaction.

[0010] Palmitoyl pentapeptide-4 is a signaling peptide that effectively promotes collagen synthesis, increases skin thickness, and reduces the number and depth of wrinkles, exhibiting significant anti-aging effects. Polydeoxyribonucleotide (PDRN), extracted from salmon testes, has 95% similarity to human DNA and is a natural cell regeneration and repair agent. PDRN activates A2A adenosine receptors on the cell surface, promoting cytokine release, significantly increasing fibroblast proliferation, and activating aging and damaged cells, thus demonstrating excellent anti-aging effects.

[0011] However, due to limitations in dosage and the poor transdermal absorption of high molecular weight peptides, skincare products containing palmitoyl pentapeptide-4 and PDRN often have poor efficacy and are prone to irritating the skin, causing allergic reactions. Palmitoyl pentapeptide-4, as an amphiphilic polypeptide, possesses the characteristic of self-assembling into nanostructures. This invention encapsulates PDRN as a core material within the self-assembled structure of palmitoyl pentapeptide-4, simultaneously increasing the maximum usable concentration of both active ingredients. Furthermore, the nanoscale particle size enhances the transdermal permeability of both substances. Simultaneously, PDRN and chitosan can bind through electrostatic interactions, allowing chitosan to encapsulate the self-assembled structure. Chitosan's inherent moisturizing, antibacterial, and penetration-enhancing properties can mitigate the skin irritation caused by high concentrations of active ingredients and further enhance the transdermal permeability of both substances.

[0012] In embodiments of the present invention, the present invention is based on the self-assembly properties of palmitoyl pentapeptide-4, and forms a biogel by loading PDRN and chitosan, wherein PDRN is encapsulated in the self-assembly structure of palmitoyl pentapeptide-4, and chitosan is coated on the outside of the self-assembly structure through electrostatic interaction, which can significantly enhance the activity of palmitoyl pentapeptide-4 and PDRN, and the effect is more significant than using either one alone or using both at the same time. In particular, the biogel composition described in this application has high compatibility, is safe and non-toxic to the human body, and will not cause irritation or allergies when applied to the skin.

[0013] In embodiments of the present invention, the mass ratio of palmitoyl pentapeptide-4 to PDRN is 1.5-30:1, at which ratio palmitoyl pentapeptide-4 can effectively encapsulate PDRN; in some embodiments of the present invention, the mass ratio of the biogel composition of palmitoyl pentapeptide-4 to PDRN is 1.5-25:1, at which ratio better encapsulation of PDRN can be achieved; furthermore, the effect is even better when the mass ratio of the biogel composition of palmitoyl pentapeptide-4 to PDRN is 3-15:1.

[0014] In some embodiments of the present invention, the biogel composition uses water as a solvent.

[0015] In the technical solution of this invention, PDRN is encapsulated in the self-assembled structure of palmitoyl pentapeptide-4, and chitosan is coated on the outside of the self-assembled structure through electrostatic interaction. This design allows the molecular weight limitation of PDRN to be overcome. In some embodiments of this invention, the molecular weight of PDRN is 0.5 to 10 million (Da). In the technical solution of this invention, low molecular weight PDRN can still achieve better results. For example, in some embodiments, the molecular weight of PDRN is 30,000 to 1 million, and even further, it is 50,000 to 500,000.

[0016] In some embodiments of the present invention, the amount of chitosan added is 1 mg / mL to 5 mg / mL.

[0017] In a second aspect of the present invention, the present invention provides a method for preparing the biogel composition described in the first aspect above, comprising: preparing a palmitoyl pentapeptide-4 solution and a PDRN solution respectively, mixing the two solutions thoroughly, adding chitosan, mixing thoroughly, and allowing to stand to obtain the biogel composition.

[0018] In embodiments of the present invention, both palmitoyl pentapeptide-4 solution and PDRN solution use water as solvent.

[0019] In an embodiment of the present invention, the preparation method further includes: preparing a palmitoyl pentapeptide-4 solution, adjusting the pH to 4.5-5, completely dissolving it, and then thoroughly mixing the palmitoyl pentapeptide-4 solution and the PDRN solution. This operation enables palmitoyl pentapeptide-4 to self-assemble and better encapsulate PDRN.

[0020] pH adjustment can be achieved using conventional methods. In some implementations, acetic acid or citric acid can be used as pH adjusters.

[0021] In some embodiments of the present invention, the concentration of palmitoyl pentapeptide-4 solution is 0.8wt%-2.5wt%; the concentration of PDRN solution is 0.1wt%-0.5wt%.

[0022] In some embodiments of the present invention, the volume ratio of palmitoyl pentapeptide-4 solution to PDRN solution is 1:1.

[0023] In some embodiments of the present invention, the amount of chitosan added is 1 mg / mL to 5 mg / mL.

[0024] In some embodiments of the present invention, the palmitoyl pentapeptide-4 solution and PDRN solution are mixed and then treated by ultrasound, and chitosan is added and mechanically stirred to mix them thoroughly.

[0025] In some embodiments of the present invention, the settling is performed at a temperature of 18-25°C under standard atmospheric pressure for 4-8 hours.

[0026] The method of this invention is based on the self-assembly properties of palmitoyl pentapeptide-4, which carries PDRN and chitosan to form a biogel. It has good stability, safety and biocompatibility, and is non-irritating and non-sensitizing when applied to the skin. It can significantly enhance the activity of palmitoyl pentapeptide-4 and PDRN, and the effect is more significant than when either one is used alone or when both are used simultaneously.

[0027] In a third aspect of the invention, the invention provides a biogel particle prepared by freeze-drying the biogel composition described in the first aspect above.

[0028] In a fourth aspect, the present invention provides a topical skin composition comprising the biogel particles described in the first aspect above.

[0029] In some embodiments of the present invention, the amount of the biogel particles added to the topical skin composition is 0.1%-5% (by mass).

[0030] In a fifth aspect of the invention, the invention provides the use of the biogel composition described in the first aspect, the biogel particles described in the third aspect, or the topical skin composition described in the fourth aspect in the preparation of skin care products.

[0031] In embodiments of the present invention, the skin care product has an anti-aging effect; in particular, it has the effect of improving rough skin and reducing wrinkles.

[0032] In some embodiments of the present invention, the skincare products include, but are not limited to, serums, lotions, masks, and creams.

[0033] Furthermore, the present invention provides the biogel composition described in the first aspect above, a skin topical composition comprising the biogel composition, or a method of using the biogel particles described in the third aspect above.

[0034] In some embodiments of the present invention, in addition to conventional methods of use (such as applying, patting, etc.), a beauty device, infrared or low-to-medium frequency instrument can be used to assist in introducing the biogel composition or a skin topical composition containing the biogel composition of the present invention into the skin.

[0035] In some embodiments of the present invention, the bio-gel particles described herein can be added to other cosmetics or used directly as an active ingredient in the preparation of cosmetics. For example, in one embodiment, the present invention provides a skin care water that uses the bio-gel particles described herein as an active ingredient, supplemented with solvents, moisturizers, and other ingredients. For products requiring long-term storage, antibacterial agents or preservatives can be added.

[0036] In a sixth aspect, the present invention provides a method for anti-aging, comprising applying to a subject an effective amount of the biogel composition described in the first aspect above, or a topical composition or skin care product comprising the biogel composition. The subject primarily refers to a person, particularly an individual with anti-aging needs or daily skincare needs.

[0037] The effective amount mainly refers to the amount of active substances or preparations, including the biogel composition described in this invention, which can elicit a biological response in humans, including reducing or partially reducing symptoms of skin aging caused by natural aging or external factors, such as rough skin and wrinkles.

[0038] Compared with existing technologies, the advantages of this invention are:

[0039] This invention provides a self-assembled biogel composition comprising palmitoyl pentapeptide-4, PDRN, and chitosan. The biogel composed of these three components exhibits good stability, safety, and biocompatibility. It is non-irritating and non-sensitizing on the skin and significantly enhances the activity of palmitoyl pentapeptide-4 and PDRN, showing greater efficacy than either component alone or both simultaneously. The biogel composition of this invention can be freeze-dried to prepare biogel particles, which can then be further used as active ingredients in skincare products, demonstrating broad application prospects.

[0040] This invention provides a method for preparing a self-assembled biogel composition that effectively coordinates palmitoyl pentapeptide-4, PDRN, and chitosan. This method fully utilizes the self-assembly of palmitoyl pentapeptide-4 to form nanostructures and leverages the interactions among the three components. This not only improves the stability, bioavailability, and transdermal absorption of the active substances but also eliminates irritation and sensitization, significantly enhancing the anti-aging effect. Attached Figure Description

[0041] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. The embodiments of this application will be described in detail below with reference to the accompanying drawings, wherein:

[0042] Figure 1 The graph shows the data results of how different test substances improved the average skin roughness SEr in Experiment Example 3.

[0043] Figure 2 The graph shows the data results of how different test substances reduced skin wrinkles (SEw) in Experiment Example 3. Detailed Implementation

[0044] The present application is further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the application. Experimental methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the manufacturer.

[0045] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of skill in the art. All reagents and materials used in this application are readily available through conventional means, and unless otherwise specified, they are used in accordance with conventional methods in the art or as per the product instructions. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to the methods of this application. The preferred embodiments and materials described herein are for illustrative purposes only.

[0046] Unless otherwise specified in the following preparation examples, the molecular weight of PDRN is 0.5 to 10 million; the solvent for the palmitoyl pentapeptide-4 solution and the PDRN solution is deionized water, and the pH of the palmitoyl pentapeptide-4 solution is 4.5-5.

[0047] Example 1 Preparation of biogels

[0048] Preparation Example 1:

[0049] Prepare a 1% palmitoyl pentapeptide-4 solution and a 0.1% PDRN solution. Mix the two solutions at a volume ratio of 1:1 and sonicate for 10 minutes.

[0050] Chitosan was added to the mixed solution obtained in the first step at a dosage of 1 mg / mL. After mechanical stirring for 40 minutes, the mixture was allowed to stand at room temperature for 8 hours to obtain a biogel. A portion of the gel was used for experiments in Example 2 and Example 3, and the remaining gel was freeze-dried to prepare gel particles.

[0051] Preparation Example 2:

[0052] Prepare a 1.5% palmitoyl pentapeptide-4 solution and a 0.3% PDRN solution. Mix the two solutions at a volume ratio of 1:1 and sonicate for 10 minutes.

[0053] Chitosan was added to the mixed solution obtained in the first step at a dosage of 2 mg / mL. After mechanical stirring for 60 minutes, the mixture was allowed to stand at room temperature for 6 hours to obtain a biogel. A portion of the gel was used for experiments in Example 2 and Example 3, and the remaining gel was freeze-dried to prepare gel particles.

[0054] Preparation Example 3:

[0055] Prepare a 2% palmitoyl pentapeptide-4 solution and a 0.5% PDRN solution. Mix the two solutions at a volume ratio of 1:1 and sonicate for 10 minutes.

[0056] Chitosan was added to the mixed solution obtained in the first step at a dosage of 4 mg / mL. After mechanical stirring for 90 minutes, the mixture was allowed to stand at room temperature for 5 hours to obtain a biogel. A portion of the gel was used for experiments in Example 2 and Example 3, and the remaining gel was freeze-dried to prepare gel particles.

[0057] Preparation of Comparative Example 1:

[0058] Prepare a 1% palmitoyl pentapeptide-4 solution by adding chitosan to the palmitoyl pentapeptide-4 solution at a dosage of 1 mg / mL and mechanically stirring for 40 minutes.

[0059] A 0.1% PDRN solution was prepared. The solution obtained in step 1 was mixed with the PDRN solution at a volume ratio of 1:1. The mixture was sonicated for 10 minutes and allowed to stand at room temperature for 8 hours to obtain a biogel. A portion of the gel was used for experiments in Example 2 and Example 3. The remaining gel was freeze-dried to prepare gel particles.

[0060] Preparation of Comparative Example 2:

[0061] A 1% palmitoyl pentapeptide-4 solution was prepared and sonicated for 30 minutes. Chitosan was added to the polypeptide solution at a dosage of 1 mg / mL. After mechanical stirring for 40 minutes, the solution was allowed to stand at room temperature for 8 hours to obtain a self-assembled polypeptide gel. A portion of the gel was used for experiments in Example 2 and Example 3. The remaining gel was freeze-dried to prepare gel particles.

[0062] Preparation of Comparative Example 3:

[0063] A 0.1% PDRN solution was prepared and sonicated for 10 minutes. Chitosan was added to the PDRN solution at a dosage of 1 mg / mL. After mechanical stirring for 40 minutes, a PDRN-chitosan mixed solution was obtained. A portion of the solution was used for experiments in Example 2 and Example 3. The remaining solution was freeze-dried to prepare lyophilized powder.

[0064] Preparation of Comparative Example 4:

[0065] A 1% palmitoyl pentapeptide-4 solution and a 0.1% PDRN solution were prepared. The two solutions were mixed at a volume ratio of 1:1, and the mixture was sonicated for 10 minutes. The mixture was then allowed to stand at room temperature for 8 hours to obtain a biogel. A portion of the gel was used for experiments in Example 2 and Example 3. The remaining gel was freeze-dried to prepare gel particles.

[0066] Example 2 Preparation of skin care water

[0067] The components and proportions of the skin care water are shown in Table 1. The components were thoroughly mixed according to the formulation shown in Table 1 to obtain the skin care water. The active ingredients were the gel particles prepared in Preparation Examples 1-3, Comparative Examples 1, 2, and 4, or the lyophilized powder prepared in Comparative Example 3. The prepared skin care waters were designated as skin care water samples 1-3 according to the serial numbers of Preparation Examples 1-3, and as skin care water control samples 1-4 according to the serial numbers of Comparative Examples 1-4.

[0068] Table 1. Formula composition of anti-aging skin care water

[0069] Components Content (percentage by mass) Active ingredients 0.5％ Butylene glycol 5％ Phenoxyethanol 0.2％ Deionized water margin

[0070] Experimental Example 1 Stability performance test

[0071] The stability of the skin care water samples 1-3 and the skin care water control samples 1-4 prepared in Example 2 was investigated.

[0072] First, the test samples were placed in a constant temperature incubator (40℃) for one month, then removed and allowed to return to room temperature before observation. Next, the test samples were placed in a refrigerator (2-8℃) for one month, then removed and allowed to return to room temperature before observation. This process was repeated three times. After each cycle, the color, precipitation, and stratification of the test samples were observed. The experimental results are shown in Table 4.

[0073] Table 4. Stability test results

[0074]

[0075] After the second cycle of the experiment, white precipitate appeared in skin care water control sample 1, and turbidity appeared in skin care water control sample 3. However, after three cycles of the experiment, skin care waters 1-3 and skin care water control samples 2 and 4 did not show any precipitation, discoloration, or stratification. When the bio-gel of this invention is formulated into skin care products such as essences, serums, masks, and creams using conventional formulations and processes, it also exhibits excellent stability.

[0076] Experimental Example 2 Skin sensitization test

[0077] According to GB / T 21608-2008 "Test Methods for Skin Sensitization of Chemicals", skin sensitization tests were conducted on the gels prepared in Preparation Examples 1-3 and Preparation Comparative Examples 1-4 to verify their safety and biocompatibility for skin use.

[0078] Ninety healthy adult albino guinea pigs (weighing 250±25g) were selected, including 45 males and 45 females (none of which had reproduced or were pregnant). 2,4-Dinitrochlorobenzene was used as the positive sensitizer. The 90 experimental guinea pigs were randomly divided into 9 groups (5 females and 5 males in each group). Twenty-four hours before the start of the experiment, the hair on both sides of the back of each experimental guinea pig was shaved, with the shaved area measuring 3cm×3cm.

[0079] Experimental Groups:

[0080] 1. Experimental group: There are 3 groups in total, namely experimental group 1, experimental group 2 and experimental group 3, which are coated with the gels prepared in preparation examples 1-3 respectively.

[0081] 2. Experimental control groups: There are 4 groups in total, namely control group 1, control group 2, control group 3 and control group 4, which are coated with the gels prepared in comparative examples 1-4 respectively.

[0082] 3. Blank control group: Samples without coating.

[0083] 4. Positive control group: coated with the positive sensitizer 2,4-dinitrochlorobenzene.

[0084] The specific experimental steps are as follows:

[0085] 1. Induced contact:

[0086] According to the experimental groups, 0.05g of the test biogel was evenly applied to the left hairless area of ​​each group of guinea pigs. 0.05mL of 1wt% 2,4-dinitrochlorobenzene was evenly applied to the left hairless area of ​​the positive control group guinea pigs, while no sample was applied to the blank control group. After application, the applied area was covered with gauze and fixed with an appropriate method for 6 hours. The experiment was repeated on the 7th and 14th days using the same method.

[0087] 2. Initiate contact:

[0088] On day 28 of the experiment, 0.05 g of the test biogel was evenly applied to the right hairless area of ​​each group of guinea pigs according to the experimental groups. 0.05 mL of 0.1 wt% 2,4-dinitrochlorobenzene was evenly applied to the right hairless area of ​​the positive control group guinea pigs. No sample was applied to the blank control group. After application, the applied area was covered with gauze and fixed with an appropriate method for 6 hours.

[0089] Six hours later, the gauze and test substance were removed, and the skin allergic reactions of each group of guinea pigs were immediately observed. The skin allergic reactions were then observed again at 24, 48, and 72 hours. The skin condition of the guinea pigs was assessed according to the "Skin Sensitization Reaction Scoring Standard," and the sensitization rate of the test substance was calculated. The appearance of skin erythema or edema (regardless of severity) was recorded as a positive skin allergic reaction. The sensitization rate was then calculated using the following formula:

[0090] Sensitization rate / % = [Number of animals with positive skin allergic reactions / Total number of test animals in the group] × 100%.

[0091] The evaluation criteria for sensitization rate are shown in Table 2, and the experimental results are shown in Table 3.

[0092] Table 2. Sensitization Rate Evaluation Criteria

[0093]

[0094]

[0095] Table 3 Experimental Results

[0096]

[0097] According to the experimental results, neither the experimental group nor the blank control group of guinea pigs coated with the gels prepared in Examples 1-3 showed positive skin allergic reactions such as erythema or edema, with a sensitization rate of 0, indicating no sensitization. The experimental guinea pigs in the positive control group all showed varying degrees of erythema or edema, with a sensitization rate of 100%, indicating extreme sensitization. A few guinea pigs coated with the gels prepared in Comparative Examples 1 and 4 showed allergic reactions, with sensitization rates of 20% and 40%, respectively. This indicates that the structural composition of the biogel affects its skin irritation, and that chitosan has a protective effect on the skin when used as the outer coating material.

[0098] Experimental Example 3 Experimental study on skin roughness improvement and wrinkle reduction

[0099] Twenty female subjects aged 30 to 50 years were randomly divided into four groups of five. After cleansing, the subjects sat quietly for 30 minutes in an environment with a room temperature of 23°C and a relative humidity of 55% until their facial muscles were relaxed. The initial roughness and initial amount of wrinkles of the subjects' skin were then measured using a VISIOSCAN VC98 skin analyzer.

[0100] After initial measurements were completed, subjects cleansed their faces morning and evening, then applied an appropriate amount of the test substance evenly to their entire face for four weeks without using any other skincare products. Skin roughness and wrinkle count were measured again on days 7, 14, and 28 of the trial.

[0101] Experimental Groups:

[0102] Test group 1: Apply the gel prepared in preparation example 1;

[0103] Test group 2: Apply the gel prepared in Comparative Example 2;

[0104] Test group 3: Apply the solution prepared in Comparative Example 3;

[0105] Blank control group: Apply purified water.

[0106] Because the gels prepared in Comparative Examples 1 and 4 showed skin sensitization, they were not included in this test.

[0107] The testing environment needs to be kept stable during the measurement. Data on average skin roughness and wrinkle amount at different times are shown in Tables 5 and 6. The effects of different test substances on improving skin roughness and reducing wrinkles are shown in Tables 5 and 6. Figure 1 and Figure 2 As shown, SEr is the average skin roughness index; the smaller the value, the less rough the skin. SEw is the skin wrinkle index; the smaller the value, the fewer the skin wrinkles.

[0108] Table 5. Average skin roughness SEr at different times

[0109]

[0110] Analysis of the test data in Table 5 shows that palmitoyl pentapeptide-4 and PDRN have the effect of improving skin roughness; combined with Figure 1 Analysis showed that after 28 days of sample use, the skin roughness of test group 1 was significantly reduced by 70.3%, and the improvement effect was significantly better than that of test group 2 (48.05%) and test group 3 (40.08%). This indicates that the biogel prepared by the present invention can effectively improve skin roughness, and the components have a synergistic effect, which is significantly better than using any one or two active ingredients alone.

[0111] Table 6. Skin wrinkle amount at different time points (SEw)

[0112]

[0113] Analysis of the test data in Table 6 shows that palmitoyl pentapeptide-4 and PDRN have the effect of reducing wrinkles; combined with Figure 2 Analysis showed that after 28 days of sample use, the skin wrinkles in test group 1 were significantly reduced by 30.15%, which was significantly better than that in test group 2 (20.81%) and test group 3 (18.13%). This indicates that the biogel prepared by the present invention can effectively reduce wrinkles and that the components have a synergistic effect, which is better than using any one or two active ingredients alone.

[0114] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A self-assembly based biogel composition comprising palmitoyl pentapeptide-4, polydeoxyribonucleotides and chitosan, wherein, The polydeoxyribonucleotides are encapsulated in the self-assembled structure of palmitoyl pentapeptide-4, and chitosan is coated on the outside of the self-assembled structure by electrostatic interaction; The mass ratio of palmitoyl pentapeptide-4 to polydeoxyribonucleotides is 3-15:1; The molecular weight of polydeoxyribonucleotides is 0.5 to 10 million; The palmitoyl pentapeptide-4 solution and the polydeoxyribonucleotide solution are prepared respectively, mixed thoroughly, and then chitosan is added and mixed thoroughly, and the mixture is left to stand, thereby obtaining the bio-gel composition; the two solutions are mixed thoroughly by ultrasonic mixing; after the addition of chitosan, the mixture is mixed thoroughly by mechanical stirring; the standing is performed at a temperature of 18-25°C under one standard atmosphere, and the standing time is 4-8h. In the preparation of the palmitoyl pentapeptide-4 solution, the pH is adjusted to 4.5-5, and after complete dissolution, the palmitoyl pentapeptide-4 solution and the polydeoxyribonucleotides are mixed thoroughly.

2. The bio-gel composition according to claim 1, characterized in that, The amount of chitosan added is 1 mg / mL-5 mg / mL.

3. A method of preparing the self-assembly based biogel composition of claim 1 or 2, comprising: The palmitoyl pentapeptide-4 solution and the polydeoxyribonucleotide solution are prepared respectively, mixed thoroughly, and then chitosan is added and mixed thoroughly, and the mixture is left to stand, thereby obtaining the bio-gel composition; the two solutions are mixed thoroughly by ultrasonic mixing; after the addition of chitosan, the mixture is mixed thoroughly by mechanical stirring; the standing is performed at a temperature of 18-25°C under one standard atmosphere, and the standing time is 4-8h. In the preparation of the palmitoyl pentapeptide-4 solution, the pH is adjusted to 4.5-5, and after complete dissolution, the palmitoyl pentapeptide-4 solution and the polydeoxyribonucleotides are mixed thoroughly.

4. The method of claim 3, wherein, The concentration of the palmitoyl pentapeptide-4 solution is 0.8 wt%-2.5 wt%, and the concentration of the polydeoxyribonucleotide solution is 0.1 wt%-0.5 wt%; The volume ratio of the palmitoyl pentapeptide-4 solution to the polydeoxyribonucleotide solution is 1:

1. The amount of chitosan added is 1 mg / mL-5 mg / mL.

5. A bio-gel particle prepared by freeze-drying of the self-assembled bio-gel composition of claim 1 or 2.

6. A skin external use composition comprising the bio-gel particle of claim 5.

7. The skin external composition according to claim 6, characterized by The amount of the bio-gel particle added to the skin external use composition is 0.1%-5% by mass.

8. Use of the bio-gel composition of claim 1 or 2, or the bio-gel particle of claim 5, or the skin external use composition of claim 6 or 7 in the preparation of a skin care product.

9. Use according to claim 8, characterized in that, The skin care product has an anti-aging effect.

10. Use according to claim 8, characterized in that, The skin care product includes an essence, a serum, a toner, a mask, and a cream.

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