Method for preventing and treating ascidian cysticercosis

Abstract

The application discloses a method for preventing and treating Hypentoma abdominalis and other parasitic diseases, which comprises the following steps: on the first day, feeding vitamin C bait after formalin immersion, and then adding bacillus subtilis in the breeding tank; on the second day, feeding vitamin C bait after formalin immersion; on the third day, feeding aquatic multi-vitamin bait after formalin immersion, and adding vitamin C in the breeding tank; on the fourth day, first adding oxytetracycline hydrochloride in the breeding tank after formalin immersion, then adding vitamin C, feeding aquatic multi-vitamin bait after vitamin C immersion, and finally adding bacillus subtilis in the breeding tank; on the fifth day, feeding aquatic multi-vitamin bait after vitamin C immersion in the breeding tank, and then immersing. The method has the advantages of short time effect, fast effect, simple operation, convenience, and the like, can effectively cure Hypentoma abdominalis and other parasitic diseases, can prevent secondary infection of molds and bacteria, can reduce stress of the seahorse, can improve immunity, can significantly reduce the mortality of Hypentoma abdominalis, and can improve the economic benefits of breeding.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of aquaculture, more particularly to a method for preventing and treating the small cucumber worm disease of the Tridacna derasa. BACKGROUND

[0002] Fish artificial breeding disease control technology plays an important role in the development process of fishery industry in China. As a national second-class protected animal, the artificial breeding disease control technology of sea horse is particularly important. As a special fish, Tridacna derasa has very important medicinal value. It is originally from the southwest Pacific Ocean and the sea area near Oceania and New Zealand. It is one of the largest species of all known Hippocampus. The study on the artificial breeding disease control method of Tridacna derasa is of great significance to the artificial breeding of Tridacna derasa.

[0003] Due to the difference in geographical environment of different places, the survival rate of artificial breeding adult Tridacna derasa and fry is low, and the disease mortality rate is high. At the same time, due to unstable feed source, pathogenic bacteria are carried, which leads to the recurrence of disease control. The traditional biochemical and Chinese medicine preparation processing technology is complex, and the drug effect is relatively slow. The small cucumber worm disease is one of the main diseases of Tridacna derasa artificial breeding, and the small cucumber worm disease has high mortality and fast transmission. The existing prevention and treatment methods for the small cucumber worm disease are relatively single, mainly focusing on artificial biochemical and Chinese medicine preparation, and the action way does not completely cover the complete life history of the small cucumber worm, which is difficult to control recurrence.

[0004] Therefore, how to provide a method for preventing and treating the small cucumber worm disease of Tridacna derasa to perfect the disease control of Tridacna derasa is a problem to be solved by those skilled in the art SUMMARY

[0005] Therefore, the present application provides a method for preventing and treating the small cucumber worm disease of Tridacna derasa, which comprehensively controls the pathogenic sea horse, pathogenic small cucumber worm and breeding water body, focuses on solving the technical problems of high mortality and easy recurrence of the small cucumber worm disease of Tridacna derasa artificial breeding, and reduces the disease mortality of breeding sea horse.

[0006] To achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0007] A method for preventing and treating the small cucumber worm disease of Tridacna derasa, comprising the following steps:

[0008] Step 1: first day treatment

[0009] The infected Tridacna derasa is placed in a fresh seawater breeding tank, formalin is added and soaked for 2h, then the sea horse is poured into fresh seawater and rested for 6h;

[0010] Vitamin C 5g / kg feed is added to the Mysis feed, mixed and placed for 30min, then fed to the Tridacna derasa, the feeding amount is 10g / kg sea horse body weight, and the residual feed is removed by suction after 1h.

[0011] After 3h of rest, the Bacillus subtilis was diluted with 10 times water and soaked for 1h, then evenly sprayed in the breeding tank and rested overnight.

[0012] Step two: treatment on the second day

[0013] After 2h of soaking in formalin, the hippocampus was poured into fresh seawater and rested for 6h.

[0014] Vitamin C was added to the Artemia feed at 5g / kg feed, mixed and rested for 30min, then fed to the P. scolyurus, with a feeding amount of 15g / kg body weight of the hippocampus. After 1h, the residual feed was removed by suction and the hippocampus was rested overnight.

[0015] Step three: treatment on the third day

[0016] After 2h of soaking in formalin, the hippocampus was poured into fresh seawater and rested for 6h.

[0017] Water product multivitamin was added to the Artemia feed at 3g / kg feed, mixed and rested for 30min, then fed to the P. scolyurus, with a feeding amount of 15g / kg body weight of the hippocampus. After 1h, the residual feed was removed by suction.

[0018] Vitamin C was taken and evenly sprayed in the breeding tank and rested overnight.

[0019] Step four: treatment on the fourth day

[0020] Oxytetracycline hydrochloride was evenly sprayed in the breeding tank after treatment on the third day, and the hippocampus was poured into fresh seawater after 8h of rest.

[0021] Vitamin C was evenly sprayed in the breeding tank and rested for 2h.

[0022] Water product multivitamin was added to the Artemia feed at 6g / kg feed, mixed and rested for 30min, then fed to the P. scolyurus, with a feeding amount of 20g / kg body weight of the hippocampus. After 1h, the residual feed was removed by suction.

[0023] Bacillus subtilis was diluted with 10 times water and soaked for 1h, then evenly sprayed in the breeding tank and rested overnight.

[0024] Step five: treatment on the fifth day

[0025] The hippocampus was poured into fresh seawater, and vitamin C was evenly sprayed in the breeding tank and rested for 8h.

[0026] Water product multivitamin was evenly sprayed in the breeding tank and rested for 2h, then Artemia feed was fed to the P. scolyurus, with a feeding amount of 20g / kg body weight of the hippocampus. After 1h, the residual feed was removed by suction and the hippocampus was rested overnight.

[0027] Preferably, the whole process in steps one to five maintains the water temperature in the breeding tank at 19 DEG C, and sufficient oxygen is supplied.

[0028] Preferably, the amount of formalin added in steps one to three is 250-350 mL / m 3 .

[0029] Preferably, the amount of Bacillus subtilis added in steps one and four is 0.8 g / m 3 .

[0030] Preferably, the vitamin C is dissolved in water before being added to the bait in steps one and two, and the water product multi-vitamin is dissolved in water before being added to the bait in steps three and four.

[0031] Preferably, the amount of vitamin C sprayed in step three is 2 g / m 3 .

[0032] Preferably, the amount of oxytetracycline hydrochloride sprayed in step four is 5 g / m 3 , and the amount of vitamin C sprayed is 0.5 g / m 3 .

[0033] Preferably, the amount of vitamin C sprayed in step five is 0.5 g / m 3 , and the amount of water product multi-vitamin sprayed is 1 g / m 3 .

[0034] Preferably, the water product multi-vitamin is dissolved in water before being added to the bait in steps three and four.

[0035] Preferably, the process in steps one to five is carried out under light during the day and in darkness at night.

[0036] The technical solution described above provides a method for preventing and treating Hypselobarbus jokuang disease, and has the following beneficial effects compared with the prior art:

[0037] The formalin solution is used to kill parasites such as small cucumbers in advance, water is changed effectively at intervals in the whole life history of the small cucumbers, the small cucumbers that have separated from the outside of the sea horse and are in the free breeding water are removed, and secondary reproduction and infection are prevented; vitamin C, oxytetracycline hydrochloride, water product multi-vitamin and Bacillus subtilis are added to the thawed bait and water, the stress resistance, growth and immunity of the sea horse are effectively improved, the drug efficacy is enhanced, the side effects of the drug are reduced, the reproduction of pathogenic bacteria is effectively inhibited, the occurrence of enteritis and rotting gills is reduced, the water quality is stabilized, the effect is remarkable after 3-5 days, and the method is a comprehensive treatment method that kills insects and sterilizes, enhances efficacy and stabilizes water, and improves the immunity of the Hypselobarbus jokuang. DETAILED DESCRIPTION

[0038] The technical solutions of the present application will be described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0039] Example 1

[0040] In the following examples, the aquatic multi-vitamins are purchased from BAYER.

[0041] Day 1: Turn on the light at 6 am, put the infected seahorses into 19℃ fresh seawater, add 300mL / m 3 of 37%-40% formaldehyde solution (formalin), soak for 2h, then pour the seahorses into a new cultivation tank (19℃ fresh seawater); at 2 pm, take 5g / kg feed of vitamin C, soak the frozen krill feed for 30min, then feed the seahorses, the feed feeding amount is 10g / kg seahorse body weight, remove the residual feed by suction after 1h, take 0.8g / m 3 of Bacillus subtilis (100 billion / g), dilute with 10 times water, soak for 1h, then evenly sprinkle, turn off the light at 7 pm, control the temperature at 19℃ all day, and oxygenate adequately;

[0042] Day 2: Turn on the light at 6 am, add 300mL / m 3 of 37%-40% formaldehyde solution (formalin) in the original tank, soak for 2h, then pour the seahorses into a new cultivation tank (19℃ fresh seawater); at 2 pm, take 5g / kg feed of vitamin C, soak the frozen krill feed for 30min, then feed the seahorses, the feed feeding amount is 15g / kg seahorse body weight, remove the residual feed by suction after 1h, turn off the light at 7 pm, control the temperature at 19℃ all day, and oxygenate adequately;

[0043] Day 3: Turn on the light at 6 am, add 300mL / m 3 of 37%-40% formaldehyde solution (formalin) in the original tank, soak for 2h, then pour the seahorses into a new cultivation tank (19℃ fresh seawater); at 2 pm, take 3g / kg feed of aquatic multi-vitamins, soak the frozen krill feed for 30min, then feed the seahorses, the feed feeding amount is 15g / kg seahorse body weight, remove the residual feed by suction after 1h, take 2g / m 3 of vitamin C, soak the water evenly, turn off the light at 7 pm, control the temperature at 19℃ all day, and oxygenate adequately;

[0044] Day 4: Turn on the light at 6 am, evenly sprinkle terramycin hydrochloride 5g / m 3; 2pm, pour Hippocampus into new culture tank (19℃ fresh seawater), take vitamin C 0.5g / m 3 Sprinkle evenly in the tank to reduce stress, take water product multi-vitamin 6g / kg bait dissolving water to soak and freeze shelled shrimp bait 30min, then feed the swollen abdomen Hippocampus, the bait feeding amount is 20g / kg Hippocampus body weight, 1h later, remove the residual bait by sucking the bottom, take bacillus subtilis (100 billion / g) 0.8g / m 3 , soak for 1h after dilution with 10 times water, then sprinkle evenly, 7pm, turn off the light, control the temperature at 19℃ all day, and oxygenate adequately;

[0045] Fifth day: 6am, turn on the light, pour Hippocampus into new culture tank (19℃ fresh seawater), take vitamin C 0.5g / m 3 Sprinkle evenly in the tank to reduce stress; 2pm, take water product multi-vitamin 1g / m 3 Sprinkle evenly in the tank, 4pm, feed the swollen abdomen Hippocampus frozen shelled shrimp bait, the bait feeding amount is 20g / kg Hippocampus body weight, 1h later, remove the residual bait by sucking the bottom, 7pm, turn off the light, control the temperature at 19℃ all day, and oxygenate adequately.

[0046] Experimental example:

[0047] Take 100 infected Hippocampus for each group to perform comparative experiments, and take 3 parallel experimental groups for each way, and the specific death conditions are as follows:

[0048]

[0049]

[0050] The above results show that the method of the present application can effectively treat Ichthyophthiriasis and greatly reduce the mortality rate.

[0051] The embodiments in the specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts of each embodiment can be referred to each other. For the device disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple, and the related parts can be referred to the method part.

[0052] The above description of the disclosed embodiments enables a person skilled in the art to implement or use the present application. Various modifications to the embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for controlling ascidian cyst disease, characterized by, The method comprises the following steps: Step one: first day treatment After the infected sea horse is soaked in fresh seawater tank with formalin for 2 hours, the sea horse is poured into fresh seawater and rested for 6 hours; Vitamin C is added into the brine shrimp bait at 5 g / kg of bait, and after being uniformly mixed and rested for 30 minutes, the sea horse is fed, and the feeding amount is 10 g / kg of sea horse weight; after 1 hour, the residual bait is removed by suction; After resting for 3 hours, Bacillus subtilis is diluted with 10 times water and soaked for 1 hour, and then uniformly sprayed in the tank and rested overnight; Step two: second day treatment After the tank after the first day treatment is soaked with formalin for 2 hours, the sea horse is poured into fresh seawater and rested for 6 hours; Vitamin C is added into the brine shrimp bait at 5 g / kg of bait, and after being uniformly mixed and rested for 30 minutes, the sea horse is fed, and the feeding amount is 15 g / kg of sea horse weight; after 1 hour, the residual bait is removed by suction and rested overnight; Step three: third day treatment After the tank after the second day treatment is soaked with formalin for 2 hours, the sea horse is poured into fresh seawater and rested for 6 hours; Aquatic multi-vitamins are added into the brine shrimp bait at 3 g / kg of bait, and after being uniformly mixed and rested for 30 minutes, the sea horse is fed, and the feeding amount is 15 g / kg of sea horse weight; after 1 hour, the residual bait is removed by suction; Vitamin C is uniformly sprayed in the tank and rested overnight; Step four: fourth day treatment After the tank after the third day treatment is uniformly sprayed with oxytetracycline hydrochloride, the sea horse is poured into fresh seawater and rested for 8 hours; Vitamin C is uniformly sprayed in the tank and rested for 2 hours; Aquatic multi-vitamins are added into the brine shrimp bait at 6 g / kg of bait, and after being uniformly mixed and rested for 30 minutes, the sea horse is fed, and the feeding amount is 20 g / kg of sea horse weight; after 1 hour, the residual bait is removed by suction; Bacillus subtilis is diluted with 10 times water and soaked for 1 hour, and then uniformly sprayed in the tank and rested overnight; Step five: fifth day treatment The sea horse is poured into fresh seawater, and vitamin C is uniformly sprayed in the tank and rested for 8 hours; Aquatic multi-vitamins are uniformly sprayed in the tank and rested for 2 hours, and then the sea horse is fed with brine shrimp bait, and the bait feeding amount is 20 g / kg of sea horse weight; after 1 hour, the residual bait is removed by suction and rested overnight.

2. The method of claim 1, wherein the method is characterized by, The water temperature in the tank is kept at 19℃ during the whole treatment process in steps one to five, and sufficient oxygen is supplied.

3. The method of claim 1, wherein the method is characterized by, The amount of formalin added in steps one to three is 250-350 mL / m of seawater 3 .

4. The method of claim 1, wherein the method is characterized by, In steps one and four, the amount of Bacillus subtilis added is 0.8 g / m3 based on seawater.

5. The method for preventing and controlling Ichthyophthirius multifiliis disease in the bloated seahorse according to claim 1, characterized in that, In steps one and two, vitamin C is dissolved in water before being added to the bait, and in steps three and four, aquatic multi-vitamins are dissolved in water before being added to the bait.

6. The method of claim 1, wherein the method is characterized by, The vitamin C sprinkling amount in step three is 2 g / m 3 .

7. The method of claim 1, wherein the method is characterized by the steps of: (a) applying the composition to the plant; (b) allowing the composition to dry; and (c) applying the composition to the plant again. The terramycin hydrochloride is sprayed at 5 g / m3 and vitamin C is sprayed at 0.5 g / m3 in step four, based on the volume of the cultured seawater. 3 .

8. The method of claim 1, wherein the method is characterized by, The vitamin C is sprayed in the step five at 0.5 g / m3 of the volume of the cultured seawater, and the aquaculture multivitamin is sprayed at 1 g / m3 of the volume of the cultured seawater 3 .

9. The method of claim 1, wherein the method is characterized by, During the treatment process in steps one to five, the process is carried out under light during the day and in darkness at night.

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