An anger modeling device based on comprehensive emotional and physical stress

Abstract

A device for modeling anger based on combined emotional and physical stress relates to the technical field of experimental animal modeling devices and includes a control system, an experimental stand, and experimental cages. Several experimental cages are suspended from the experimental stand, connected to a swing mechanism and driven to swing back and forth by the swing mechanism. An intelligent striking mechanism is provided within the experimental cages, integrating a striking block, a ventilator, a water sprayer, and an air blower. The present invention can disrupt the living habits of mice and rats in various ways, making them irritable through vibrations, striking, air blowing, water spraying, and sleep disruption. An anger model in mice and rats is successfully prepared under multiple pressures, saving manpower and time, reducing operations, reducing human interference, and providing a unified and objective modeling method with accurate experimental results. This device is of reference value for research on the pathogenesis of anger and new drug development, and is worthy of further development and promotion.

Description

Technical Field

[0001] The invention relates to the technical field of experimental animal modeling devices, and in particular to an anger modeling device based on comprehensive emotional and physical stress. Background Art

[0002] Unlike other animal models, there are fewer research reports on anger stress animal models at home and abroad, and they are not as popular as the chronic unpredictable stress (CUMS)-induced depression model. There are also relatively few researchers studying them, which greatly limits the research progress of basic science.

[0003] Current research methods are as follows: ① Cat Model: Electrical stimulation of the cat's anger center and destruction of the bilateral diaphragmatic region in rats have been used to replicate an animal model of "anger-induced liver damage." The animals exhibit behaviors such as biting, staring, and confrontation. However, the aforementioned definition of anger is merely descriptive and lacks quantitative evaluation criteria. ② Restraint Stress Model: The restraint stress model is used to create a model of "anger-induced liver damage," inducing a chronic stress response. However, this method is more likely to induce depression and anxiety rather than anger. ③ Housing-Intrusion Paradigm: This model of anger stress is similar to human aggression, but lacks quantitative evaluation criteria and requires visual observation, which is highly subjective. ④ Macaque Model: This primarily utilizes social hierarchy stress, essentially involving caged fighting. However, due to the high cost of macaques and the resulting maiming caused by excessive fighting, this method is now largely discontinued. ⑤ Empty Bottle Stimulation: This can induce a certain degree of aggressiveness in rats, but the success rate of model establishment is low, the model's stability and sensitivity are poor, and there are numerous uncontrollable factors. Further exploration of model establishment conditions is needed. ⑥ Tail clamping: Clamping the mouse's tail for 1-3 minutes. This method involves physical stimulation of the animal. While it can produce intense behavioral responses of anger, it differs significantly from the psychological stress of anger in humans. ⑦ Drug-induced modeling: Inducing aggressive behavior can easily induce a direct physiological stress response, creating a pattern that confuses psychological and physiological stress, resulting in lower validity and affecting the rigor of the research results.

[0004] The testing methods mainly include: ① Brush test: The number of times mice attack or flee the brush while they are in contact with it. ② Aggression scoring: The number of attacks, attack duration, bites, climbs, climb duration, and piloerection of the rats are manually recorded visually. This is highly subjective and requires significant labor and resources.

[0005] From the above, we can see that there is currently a lack of specialized anger modeling devices, the modeling methods are confusing, and there is a lack of specialized software to analyze and evaluate anger. Summary of the Invention

[0006] The present invention provides an anger modeling device based on comprehensive emotional and physical stress, with the aim of solving the problem described in the background technology section that "there is currently a lack of specialized anger modeling devices, confusing modeling methods, and a lack of specialized software for analyzing and evaluating anger."

[0007] In order to achieve the above object, the technical solution of the present invention is:

[0008] An anger modeling device based on comprehensive emotional and somatic stress includes a control system, an experimental rack, and an experimental cage. Several experimental cages are hung on the experimental rack, and the experimental cages are connected to a swing mechanism and swing back and forth under the drive of the swing mechanism. An intelligent striking mechanism is provided in the experimental cage, and the intelligent striking mechanism integrates a striking block, a ventilation pipe, a water spray pipe, and a blow pipe. The striking block is used to apply striking stimulation to the experimental animal, the ventilation pipe is used to introduce irritating gas to the experimental animal, the water spray pipe is used to spray hot or cold water to the experimental animal, and the blow pipe is used to blow cold or hot air to the experimental animal. An infrared camera for capturing the movement trajectory of the experimental animal is provided in the experimental cage. The control system is electrically connected to the power supply and the infrared camera via wires, and is configured to control the swing mechanism, the striking block, the ventilation pipe, the water spray pipe, and the blow pipe.

[0009] Preferably, the experimental frame includes a rectangular frame and a plurality of transverse partitions evenly distributed in the rectangular frame, the transverse partitions divide the interior of the rectangular frame into a plurality of experimental spaces, the swing mechanism includes a rotating shaft arranged horizontally in the experimental space, one end of the rotating shaft is rotatably connected to the side wall of the rectangular frame, and the other end can be rotatably passed through the other side wall of the rectangular frame and fixedly connected to the output shaft end of the swing motor preset outside the rectangular frame, the swing motor is electrically connected to the control system through a wire, two hanging rings are provided side by side at the top of the experimental cage, the rotating shaft passes through the two hanging rings and is fixedly connected to the inner wall of the hanging ring; a plurality of experimental cages are hung side by side in each experimental space.

[0010] Preferably, the top of the experimental cage is detachably and fixedly connected to a top cover, the front side wall of the experimental cage is provided with a cage door, the left and right side walls of the experimental cage are respectively penetrated and embedded with a food bottle and a water bottle, a glass bottom plate is fixedly provided at the lower part of the experimental cage, and an infrared camera is fixedly provided on the middle part of the upper surface of the bottom plate of the experimental cage below the glass bottom plate, the side walls of the experimental cage are made of non-light-transmitting material, and the glass bottom plate is made of germanium glass.

[0011] Preferably, a square-shaped supporting block is fixedly provided on the upper part of the inner wall of the experimental cage, and the intelligent striking mechanism includes a limit plate clamped on the supporting block, and a plurality of first electric push rods are arranged in a matrix at the bottom end of the limit plate. The first electric push rod is arranged longitudinally, and the fixed end of the first electric push rod is fixedly connected to the lower surface of the limit plate, and the telescopic end is fixedly connected to the striking block. The side walls of the striking block are respectively fixedly connected with three hard L-shaped connecting tubes, and the vertical sections of the L-shaped connecting tubes extend upward and can slide through the sliding holes preset on the limit plate and connect with the sliding The hole is connected for sliding, the horizontal section of the L-shaped connecting pipe extends inward and enters the interior of the striking block, the end of the horizontal section extends downward through the bottom end of the striking block and respectively constitutes an air outlet or an air outlet or a water outlet, and the three L-shaped connecting pipes respectively constitute a vent pipe, a blowing pipe, and a water spray pipe, and the tops of the blowing pipe and the water spray pipe are respectively provided with a first circular plate and a second circular plate from bottom to top, the outer diameter of the second circular plate is larger than that of the first circular plate, and an elastic rubber bag is connected between the first circular plate and the second circular plate, and the tops of the blowing pipe and the water spray pipe respectively penetrate the first circular plate and enter the elastic The rubber bag is fixedly connected to the first circular plate, the top of the second circular plate is respectively provided with an air inlet pipe or a water inlet pipe, the air inlet pipe or the water inlet pipe is respectively provided with a first one-way valve that only allows air or water to flow into the elastic rubber bag, and the lower ends of the blowing pipe and the water spraying pipe are respectively provided with a second one-way valve that only allows air or water to flow outward; the outer periphery of the first electric push rod is distributed with 4 infrared sensors in a rectangular shape, and the second electric push rod is respectively provided on both sides of the upper surface of the limit plate above the first electric push rod, and the fixed end of the second electric push rod is connected to the limit plate The surface is fixedly connected, and the telescopic ends are fixedly connected with an extrusion ring. The outer diameter of the first circular plate is smaller than the inner diameter of the extrusion ring. When the second electric push rod is contracted to a set amplitude, the extrusion ring and the elastic rubber bag do not act, and only the striking block performs the striking action under the drive of the first electric push rod. When the second electric push rod is extended to a certain amplitude, the extrusion ring and the elastic rubber bag are used in conjunction with each other to realize the output of hot air or cold air or hot water or cold water during extrusion; the first electric push rod, the second electric push rod, and the infrared sensor are electrically connected to the control system through wires respectively.

[0012] Preferably, the top of the air inlet pipe in the same row of experimental cages is connected to the air supply pipe through a first flexible connecting pipe, one end of the air supply pipe is fixedly connected to the side wall of the experimental cage, and the other end passes through the outer wall of the experimental cage and is respectively connected to the cold air supply device and the hot air supply device through a first three-way joint, and the first three-way joint is respectively provided with a first solenoid valve and a second solenoid valve for controlling the supply of cold air or hot air, and the first solenoid valve, the second solenoid valve, the cold air supply device, and the hot air supply device are respectively electrically connected to the control system through wires.

[0013] Preferably, the top end of the water inlet pipe in the experimental cage in the same row is connected to the water inlet main pipe through a second flexible connecting pipe, one end of the water inlet main pipe is fixedly connected to the side wall of the experimental cage, and the other end passes through the outer wall of the experimental cage and is respectively connected to the cold water supply device and the hot water supply device through a second three-way joint, and the second three-way joint is respectively provided with a third solenoid valve and a fourth solenoid valve for controlling the supply of cold water or hot water, and the third solenoid valve, the fourth solenoid valve, the cold water supply device, and the hot water supply device are respectively electrically connected to the control system through wires.

[0014] Preferably, the top ends of the ventilation tubes in the same row are connected to a gas supply pipe through a third flexible connecting pipe, and a fifth solenoid valve is provided on the third flexible connecting pipe. One end of the gas supply pipe is fixedly connected to the inner wall of the experimental cage, and the other end passes through the outer wall of the experimental cage and is connected to the stimulation gas supply device. The fifth solenoid valve and the stimulation gas supply device are electrically connected to the control system through wires respectively.

[0015] Preferably, the lower edge of the top cover is embedded in the top of the experimental cage and is detachably fixedly connected to the top of the experimental cage by positioning bolts, the left and right inner walls of the glass bottom plate are respectively provided with slopes, and the side walls of the experimental cage at the rear side of the glass bottom plate are respectively provided with protective nets for blocking experimental animals and allowing the experimental animal feces to pass through, and a guide sleeve is fixedly provided on the rear wall surface of the experimental cage at the rear side of the protective net, and damping guide rails are respectively provided on both sides of the bottom end of the experimental cage, and a drawer with an open front end is inserted on the damping guide rail, and the open end of the drawer is sealed and docked with the lower port of the guide cover and is used to collect feces and urine of experimental animals.

[0016] Preferably, the striking block is a cylindrical metal structure, and a membrane pressure sensor is provided on the outer periphery of the striking block. The membrane pressure sensor is electrically connected to the control system through a wire, and is used to detect the number and intensity of the experimental animal biting the striking block due to anger. The control system is provided with a timing module, which records the latency and continuous biting time of the experimental animal biting the striking block based on the visual information of the infrared camera. The latency refers to the time from the first hitting of the experimental animal to the first biting of the striking block.

[0017] Preferably, the control system is electrically connected to a human-computer interaction device via a wire, and the human-computer interaction device is configured with an operation interface. The operation interface is provided with an experimental condition input window, an experimental data display window, and an experimental result analysis display window. The experimental condition input window, the experimental data display window, and the experimental result analysis display window are respectively connected to the control module, storage module, and data analysis and processing module preset in the control system.

[0018] The anger modeling device based on emotional and physical comprehensive stress of the present invention has the following beneficial effects:

[0019] The present invention can interfere with the living habits of mice and rats from various aspects, making them irritable by shaking, hitting, blowing, spraying water, disturbing their sleep, etc., and successfully preparing a model of anger in mice and rats under multiple pressures. It has the advantages of saving manpower, saving time, reducing operations, reducing human interference, unified modeling methods, objective modeling methods, and accurate experimental results. This device has reference significance for research on the pathogenesis of anger and the development of new drugs, and is worthy of further research and development and promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 is a schematic cross-sectional view of the experimental cage of the present invention;

[0022] FIG3 is a schematic diagram of the front view of the experimental cage of the present invention;

[0023] Figure 4 , a side view schematic diagram of the experimental cage of the present invention;

[0024] Figure 5 , a bottom view structural diagram of the limiting plate of the present invention;

[0025] Figure 6 , a schematic diagram of the partially enlarged structure of location A of the present invention;

[0026] 1. Rectangular frame; 2: Horizontal partition; 3. Experimental cage; 3-1. Hanging ring; 3-2. Top cover; 3-3. Positioning bolt; 3-4. Water inlet main pipe; 3-5. Limiting plate; 3-6. Support fastener; 3-7. Food bottle; 3-8. Drinking bottle; 3-9. Protective net; 3-10. Glass bottom plate; 3-11. Infrared camera; 3-12. Drawer; 4. Rotating shaft; 5. Swing motor; 6. Cage door; 7. Guide sleeve; 8. First electric push rod; 9. Striking block; 10. Water outlet; 11. Air outlet; 12. Infrared sensor; 13. Sliding hole; 14. Water spray pipe; 15. Blowing pipe; 16. Elastic rubber bag; 17. First circular plate; 18. Second electric push rod; 19. Extrusion ring; 20. Air inlet pipe; 21. Second circular plate; 22. Second flexible connecting pipe. DETAILED DESCRIPTION

[0027] The following describes in detail the implementation methods of the present invention in a step-by-step manner. This description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

[0028] In the description of the present invention, it should be noted that the terms "up", "down", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings. They are only for describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, and a specific orientation structure and operation. Therefore, they cannot be understood as limiting the present invention.

[0029] Example 1

[0030] An anger modeling device based on emotional and physical comprehensive stress, such as Figure 1-6 As shown, it includes a control system (not shown in the figure), an experimental rack, and an experimental cage 3. Several experimental cages 3 are hung on the experimental rack. The experimental cages 3 are connected to a swing mechanism and swing back and forth under the drive of the swing mechanism. An intelligent striking mechanism is provided in the experimental cage 3. The intelligent striking mechanism integrates a striking block 9, a ventilation pipe (not shown in the figure, refer to a water spray pipe), a water spray pipe 14, and a blow pipe 15. The striking block 9 is used to apply striking stimulation to the experimental animals, the ventilation pipe is used to introduce irritating gas to the experimental animals, the water spray pipe 14 is used to spray hot water or cold water to the experimental animals, and the blow pipe 15 is used to blow cold air or hot air to the experimental animals. An infrared camera 3-11 for shooting the movement trajectory of the experimental animals is provided in the experimental cage 3. The control system is electrically connected to the power supply and the infrared camera 3-11 through wires, and is configured to control the swing mechanism, the striking block, the ventilation pipe, the water spray pipe, and the blow pipe.

[0031] In this embodiment, the experimental animals are rats or mice.

[0032] In this embodiment, the rocking mechanism rocked the experimental cage, and the small rocking constituted an oscillation state to simulate uncontrollable unpleasant stimulation from the outside world, causing the experimental animals to feel angry. Of course, the rocking mechanism of the present invention can also be replaced by a horizontal vibration mode, which will not be described in detail here.

[0033] In this embodiment, the striking block can be activated according to the position of the rat, constantly poking the rat's back, interfering with the rat's free movement, irritating it, and inducing anger. The striking stimulation can also disrupt the rat's sleep, further enhancing the modeling of anger.

[0034] In this embodiment, the mice are stimulated to anger by simulating irregular stimulation of hot and cold air or cold and hot water; the irregular stimulation is not truly irregular, but is presented as irregularly as possible through the setting program of the control system so that the mice cannot adapt.

[0035] In this embodiment, the irritant gas refers to a smelly but non-toxic chemical substance that causes the experimental animals to experience disgust and induce anger by irregularly releasing one or more gas mixtures of dimethylamine (similar to a fishy smell), ethyl mercaptan (similar to a gas smell), and hydrogen disulfide (similar to a foul smell).

[0036] Among them, the specific implementation method is:

[0037] (1) Swing mechanism simulated oscillation: duration is 7 minutes, implemented 4 times a day, and the specific frequency is set according to needs.

[0038] (2) Striking: duration 2 minutes, 30 strikes, 4 times a day.

[0039] (3) Blowing air: duration 5 minutes, 6 times a day, 4 times of cold air (4 degrees air), 2 times of hot air (60 degrees air).

[0040] (4) Water spraying: Spray water 20 times within 20 minutes, 5 ml each time, for a total of 100 ml. This should be done twice a day, once with cold and once with hot water.

[0041] (5) Odor release: duration 5 minutes, implemented 3 times a day, dimethylamine, ethyl mercaptan, and hydrogen disulfide are released randomly according to the control system program.

[0042] The above content lasted for 21 days, and five types of stimuli, including shock, hitting, blowing, water spraying, and odor release, were applied daily. By creating unpredictable and uncertain stimuli, the anger emotion model of mice and rats was achieved.

[0043] Example 2

[0044] Based on Example 1, this embodiment discloses:

[0045] like Figure 1-6 As shown, the experimental frame includes a rectangular frame 1 and a number of transverse partitions 2 evenly distributed in the rectangular frame 1. The transverse partitions 2 divide the interior of the rectangular frame 1 into a number of experimental spaces. The swing mechanism includes a rotating shaft 4 arranged horizontally in the experimental space. One end of the rotating shaft 4 is rotatably connected to the side wall of the rectangular frame 1, and the other end can rotatably pass through the other side wall of the rectangular frame 1 and is fixedly connected to the output shaft end of the swing motor 5 preset outside the rectangular frame 1. The swing motor 5 is electrically connected to the control system through a wire. Two hanging rings 3-1 are arranged side by side on the top of the experimental cage 3. The rotating shaft 4 passes through the two hanging rings 3-1 and is fixedly connected to the inner wall of the hanging ring 3-1. Figure 1 As shown, several experimental cages are hung in each experimental space, thereby achieving synchronous anger emotion modeling of batch animals.

[0046] In this embodiment, the swing motor can not only simulate the oscillation state of the experimental cage, but also interfere with the position of the experimental animal by swinging the experimental cage, so that the experimental animal cannot hide in the dead corners of the experimental cage or positions that cannot be stimulated, and passively accepts various stimuli.

[0047] Example 3

[0048] Based on Example 2, this embodiment discloses:

[0049] like Figure 1-6 As shown, the top of the experimental cage 3 is detachably fixedly connected with a top cover 3-2, the front side wall of the experimental cage 3 is provided with a cage door 6, and the left and right side walls of the experimental cage 3 are respectively penetrated and embedded with a food bottle 3-7 and a water bottle 3-8. A glass bottom plate 3-10 is fixedly provided at the lower part of the experimental cage 3, and an infrared camera 3-11 is fixedly provided on the middle part of the upper surface of the bottom plate of the experimental cage below the glass bottom plate 3-10. The side walls of the experimental cage 3 are made of non-light-transmitting material, and the glass bottom plate is made of germanium glass, which facilitates the infrared light of the infrared camera to pass through and collect the movement trajectory of the experimental animal.

[0050] In this embodiment, the food and water bottles, as well as the snap-on connection between them and the experimental cage, are commonly used and will not be described in detail. The cage door is used to access the large mice, while the glass bottom supports them and transmits infrared light for the infrared camera to capture. The infrared camera can capture the mouse's movement trajectory in a dark environment. Based on the mouse's movement trajectory, the control system can assist in controlling the mouse's position through a rocking mechanism.

[0051] Example 4

[0052] Based on Example 3, this embodiment discloses:

[0053] like Figure 2As shown, a square frame-shaped supporting block 3-6 is fixedly provided on the upper part of the inner wall of the experimental cage 3, and the intelligent striking mechanism includes a limit plate 3-5 clamped on the supporting block, and a plurality of first electric push rods 8 are arranged in a matrix at the bottom end of the limit plate 3-5. The first electric push rods 8 are arranged longitudinally, and the fixed end of the first electric push rod 8 is fixedly connected to the lower surface of the limit plate 3-5, and the telescopic end is fixedly connected to the striking block 9. The side walls of the striking block 9 are respectively fixedly connected with three hard L-shaped connecting tubes, and the vertical section of the L-shaped connecting tube extends upward and can slide through the sliding hole 13 preset on the limit plate 3-5 and is slidably connected to the sliding hole 13. The horizontal section of the L-shaped connecting pipe extends inward and enters the interior of the striking block 9. The end of the horizontal section extends downward through the bottom end of the striking block and respectively constitutes an air outlet, an air outlet, or a water outlet 10. The three L-shaped connecting pipes respectively constitute a ventilation pipe (not shown in the figure), a blowing pipe 15, and a water spray pipe 14. The tops of the blowing pipe and the water spray pipe are respectively provided with a first circular plate 17 and a second circular plate 21 from bottom to top. The outer diameter of the second circular plate 21 is larger than that of the first circular plate 17. An elastic rubber bag 16 is connected between the first circular plate 17 and the second circular plate 21. The tops of the blowing pipe 15 and the water spray pipe 14 respectively penetrate the first circular plate 17 and enter the elastic rubber bag The top of the second circular plate 21 is respectively provided with an air inlet pipe 20 or a water inlet pipe (not marked in the figure), and the air inlet pipe 20 or the water inlet pipe is respectively provided with a first one-way valve (not marked in the figure) that only allows air flow or water to flow into the elastic rubber bag. The lower ends of the blowing pipe 15 and the water spray pipe 14 are respectively provided with a second one-way valve (not marked in the figure) that only allows air or water to flow outward; the outer periphery of the first electric push rod 8 is distributed with four infrared sensors 12 in a rectangular shape, and the upper surfaces of the limit plates 3-5 above the first electric push rod 8 are respectively provided with second electric push rods 18 on both sides, and the second electric push rods The fixed end of 18 is fixedly connected to the upper surface of the limit plate 3-5, and the telescopic end is fixedly connected to the extrusion ring 19. The outer diameter of the first circular plate 17 is smaller than the inner diameter of the extrusion ring 19. When the second electric push rod 18 shrinks to the set amplitude, the extrusion ring 19 and the elastic rubber bag 16 do not act, and only the striking block performs the striking action under the drive of the first electric push rod 8. When the second electric push rod 18 is extended to a certain amplitude, the extrusion ring is used in conjunction with the elastic rubber bag 16 to achieve the output of hot air or cold air or hot water or cold water during extrusion; the first electric push rod, the second electric push rod, and the infrared sensor are electrically connected to the control system through wires.

[0054] In this embodiment, by removing the top cover, the limit plate can be placed on the support block to achieve a snap connection. The first electric push rod arranged in a matrix is ​​used to drive the striking block to move, and at the same time, it can drive the three L-shaped connecting pipes to move up and down along the slide hole. During the process of the vertical sections of the blowing pipe 15 and the water spray pipe 14 moving up and down along the slide hole, if only striking stimulation is applied, the second electric push rod is retracted to a set amplitude. At this time, even when the striking block is at the lowest position, the extrusion ring 19 and the elastic rubber capsule 16 are separated from each other, so the elastic rubber capsule 16 is not squeezed. If it is desired to apply water spray stimulation or blowing stimulation, the second electric push rod is extended so that the elastic rubber capsule can enter the extrusion ring along with the first circular plate during the downward movement and be squeezed by the extrusion ring, thereby achieving the output of hot air or cold air or hot water or cold water.

[0055] In this embodiment, the infrared sensor is used to detect the position of the rat. When the rat moves to the bottom of the corresponding striking block, stimulation such as striking, blowing, spraying water, and stimulating gas can be initiated. Assisted by the rocking mechanism, the rat can be promoted to move to the position receiving the stimulation (the rat is forced to move because it is unstable in its standing position). The visual information from the infrared camera can instruct the rocking mechanism to move at the appropriate time. For example, a rat at the edge of the experimental cage can be returned to the middle of the experimental cage by rocking it.

[0056] Example 5

[0057] Based on Example 4, this embodiment discloses:

[0058] like Figure 2 、 5 As shown in Figures 6 and 7, the top of the air inlet pipe 20 in the experimental cage 3 in the same row is connected to the air supply pipe (not shown in the figure, refer to the relevant structure of the water spray pipe) through a first soft connecting pipe (not shown in the figure, refer to the relevant structure of the water spray pipe). One end of the air supply pipe is fixedly connected to the side wall of the experimental cage 3, and the other end passes through the outer wall of the experimental cage and is respectively connected to the cold air supply device (not shown in the figure) and the hot air supply device (not shown in the figure) through a first three-way joint (not shown in the figure). The first three-way joint is respectively provided with a first solenoid valve (not shown in the figure) and a second solenoid valve (not shown in the figure) for controlling the supply of cold air or hot air. The first solenoid valve, the second solenoid valve, the cold air supply device, and the hot air supply device are respectively electrically connected to the control system through wires.

[0059] In this embodiment, if cold wind stimulation is to be applied, the first solenoid valve is opened in advance, and the cold wind supply device inputs a fixed amount of cold air into the air supply pipe. The cold air enters the corresponding elastic rubber bag through the first one-way valve, wherein the corresponding second one-way valve can be made into a cross-shaped valve structure, and the valve structure can only be opened under a certain pressure. When the stimulation is applied, the elastic rubber bag is driven downward by the first electric push rod and enters the extrusion ring. After being squeezed, cold air is ejected from the air outlet. The operation method of hot wind stimulation is the same. When the rat moves due to the cold wind or hot wind stimulation, after the infrared sensor on the periphery of the corresponding first electric push rod detects the position information of the rat, the cold wind or hot wind stimulation can continue to be applied through the first electric push rod, and the elastic rubber bag that has been stimulated can continue to be replenished with cold air or hot air.

[0060] Example 6

[0061] Based on Example 5, this embodiment discloses:

[0062] like Figure 2 、 5 As shown in Figures 6 and 7, the top of the water inlet pipe in the experimental cage in the same row is connected to the water inlet main pipe 3-4 through the second flexible connecting pipe 22. One end of the water inlet main pipe 3-4 is fixedly connected to the side wall of the experimental cage 3, and the other end passes through the outer wall of the experimental cage and is respectively connected to the cold water supply device (not shown in the figure) and the hot water supply device (not shown in the figure) through the second three-way joint (not shown in the figure). The second three-way joint is respectively provided with a third solenoid valve (not shown in the figure) and a fourth solenoid valve (not shown in the figure) for controlling the supply of cold water or hot water. The third solenoid valve, the fourth solenoid valve, the cold water supply device, and the hot water supply device are respectively electrically connected to the control system through wires.

[0063] The specific principles of this embodiment are similar to those of embodiment 5 and will not be repeated here. The cold or hot water supply device may deliver cold or hot water by pumping, and the control system controls the pump.

[0064] Example 7

[0065] Based on Example 6, this embodiment discloses:

[0066] like Figure 2 、 5 As shown in Figures 6 and 7, the top ends of the ventilation tubes in the same row are connected to a gas supply pipe (not shown) through a third flexible connecting pipe (not shown in the figure). The third flexible connecting pipe is provided with a fifth solenoid valve (not shown in the figure). One end of the gas supply pipe is fixedly connected to the inner wall of the experimental cage 3, and the other end passes through the outer wall of the experimental cage and is connected to the stimulation gas supply device (not shown in the figure). The fifth solenoid valve and the stimulation gas supply device are electrically connected to the control system through wires.

[0067] In this embodiment, the stimulating gas supply device can be a gas cylinder filled with stimulating gas. An electromagnetic flow control valve can be installed at the port of the gas cylinder, and the control system controls the electromagnetic flow control valve. By replacing different gas cylinders, various stimulating gases can be delivered. During gas delivery, the gas is delivered into the experimental cage through the gas supply pipe, the third flexible connecting pipe, the vent pipe, and the gas outlet, applying gas stimulation to the mice at the gas outlet.

[0068] Example 8

[0069] Based on Example 7, this embodiment discloses:

[0070] like Figure 2 、 4 As shown in Figures 5 and 6, the lower edge of the top cover 3-2 is embedded in the top of the experimental cage 3 and is detachably fixedly connected to the top of the experimental cage 3 by a positioning bolt 3-3. The left and right inner walls of the glass bottom plate 3-10 are respectively provided with a slide (not marked in the figure, used to make the mice unstable and prompt them to return to the area where the beating stimulation is applied). On the side walls of the experimental cage 3 at the rear side of the glass bottom plate, there are respectively provided with a protective net 3-9 for blocking the experimental animals and allowing the experimental animals' feces to pass through. A guide sleeve 7 is fixedly provided on the rear wall surface of the experimental cage 3 at the rear side of the protective net 3-9. Damping guide rails (not marked in the figure) are respectively provided on both sides of the bottom end of the experimental cage 3. A drawer 3-12 with an open front end is inserted on the damping guide rail. The open end of the drawer 3-12 is sealed and docked with the lower port of the guide cover 7 and is used to collect feces and urine of the experimental animals.

[0071] In this embodiment, because the mice and rats produce feces and urine during the experiment, accumulation can interfere with the infrared camera's image capture. Therefore, a rocking mechanism is activated to tilt the cage, allowing the feces and urine to slide toward the guide sleeve and into the drawer. Water spraying is also used to facilitate the entry of feces and urine into the drawer. After the experiment, the drawer is opened, the trash is dumped, the mice and rats are removed, and the glass bottom is cleaned.

[0072] Example 9

[0073] Based on Example 8, this embodiment discloses:

[0074] The striking block 9 is a cylindrical metal structure, and a membrane pressure sensor (not shown in the figure) is provided on the outer periphery of the striking block 9. The membrane pressure sensor is electrically connected to the control system through a wire, and is used to detect the number and intensity of the experimental animals biting the striking block due to anger. The control system is provided with a timing module, which records the latency and continuous biting time of the experimental animals biting the striking block based on the visual information of the infrared camera. The latency refers to the time from the first hitting of the experimental animal to the first biting of the striking block.

[0075] Example 10

[0076] Based on Example 9, this embodiment discloses:

[0077] like Figure 1-6 As shown, the control system is electrically connected to a human-computer interaction device (not shown) via a wire. The human-computer interaction device is provided with an operation interface. The operation interface is provided with an experimental condition input window, an experimental data display window, and an experimental result analysis display window. The experimental condition input window, the experimental data display window, and the experimental result analysis display window are respectively connected to the control module, the storage module, and the data analysis and processing module preset in the control system.

[0078] In this embodiment, the input items of the experimental condition input window can be specifically set according to the experimental requirements, such as the type of mice, weight, and implementation plans of various stimuli; the experimental data display window is used to display various data during the experiment, such as the latency of biting, the number of bites, the duration of biting, and the intensity of biting; the experimental result analysis display window is used to display the analysis results of the anger emotion modeling of mice, such as the time when the mice obviously show anger, and the various stimuli implemented to achieve the anger emotion, and then analyze the causal relationship between various experimental conditions and the anger emotion modeling.

Claims

1. An anger modeling device based on combined emotional and physical stress, characterized by: The apparatus comprises a control system, an experimental rack, and an experimental cage. Several experimental cages are hung on the experimental rack, and the experimental cages are connected to a swing mechanism and swing back and forth under the drive of the swing mechanism. An intelligent striking mechanism is provided in the experimental cage, and the intelligent striking mechanism integrates a striking block, a ventilating pipe, a water spraying pipe, and an air blow pipe. The striking block is used to apply striking stimulation to the experimental animals, the ventilating pipe is used to introduce irritating gas to the experimental animals, the water spraying pipe is used to spray hot or cold water to the experimental animals, and the air blow pipe is used to blow cold or hot air to the experimental animals. An infrared camera for photographing the movement trajectory of the experimental animals is provided in the experimental cage. The control system is electrically connected to the power supply and the infrared camera through wires, and is configured to control the swing mechanism, the striking block, the ventilating pipe, the water spraying pipe, and the air blow pipe. A square-shaped supporting block is fixedly provided on the upper part of the inner wall of the experimental cage, and the intelligent striking mechanism includes a limit plate clamped on the supporting block, and a plurality of first electric push rods are arranged in a matrix at the bottom end of the limit plate. The first electric push rods are arranged longitudinally, and the fixed end of the first electric push rod is fixedly connected to the lower surface of the limit plate, and the telescopic end is fixedly connected to the striking block. The side walls of the striking block are respectively fixedly connected with three hard L-shaped connecting tubes, and the vertical sections of the L-shaped connecting tubes extend upward and can slide through the sliding holes preset on the limit plate and slide with the sliding holes. The horizontal section of the L-shaped connecting pipe extends inward and enters the interior of the striking block, and the end of the horizontal section extends downward through the bottom end of the striking block and respectively constitutes an air outlet, an air outlet, or a water outlet. The three L-shaped connecting pipes respectively constitute a vent pipe, an air blowing pipe, and a water spray pipe. The top ends of the air blowing pipe and the water spray pipe are respectively provided with a first circular plate and a second circular plate from bottom to top. The outer diameter of the second circular plate is larger than that of the first circular plate. An elastic rubber bag is connected between the first circular plate and the second circular plate. The top ends of the air blowing pipe and the water spray pipe respectively penetrate the first circular plate and enter the elastic rubber bag. The air inlet pipe or the water inlet pipe is respectively provided at the top of the second circular plate, and the air inlet pipe or the water inlet pipe is respectively provided with a first one-way valve that only allows air or water to flow into the elastic rubber bag. The lower ends of the blowing pipe and the water spraying pipe are respectively provided with a second one-way valve that only allows air or water to flow outward; the outer periphery of the first electric push rod is distributed with 4 infrared sensors in a rectangular shape, and the upper surface of the limit plate above the first electric push rod is respectively provided with a second electric push rod on both sides, and the fixed end of the second electric push rod is connected to the upper surface of the limit plate The two surfaces are fixedly connected, and the telescopic ends are fixedly connected with an extrusion ring. The outer diameter of the first circular plate is smaller than the inner diameter of the extrusion ring. When the second electric push rod is contracted to a set amplitude, the extrusion ring and the elastic rubber bag do not act, and only the striking block performs the striking action under the drive of the first electric push rod. When the second electric push rod is extended to a certain amplitude, the extrusion ring and the elastic rubber bag are used in conjunction with each other to realize the output of hot air or cold air or hot water or cold water during extrusion; the first electric push rod, the second electric push rod, and the infrared sensor are electrically connected to the control system through wires respectively.

2. The anger modeling device based on combined emotional and physical stress according to claim 1, characterized in that: The experimental frame includes a rectangular frame and several transverse partitions evenly distributed in the rectangular frame. The transverse partitions divide the interior of the rectangular frame into several experimental spaces. The swing mechanism includes a rotating shaft arranged horizontally in the experimental space. One end of the rotating shaft is rotatably connected to the side wall of the rectangular frame, and the other end can rotatably pass through the other side wall of the rectangular frame and is fixedly connected to the output shaft end of a swing motor preset outside the rectangular frame. The swing motor is electrically connected to the control system through a wire. Two hanging rings are provided side by side at the top of the experimental cage. The rotating shaft passes through the two hanging rings and is fixedly connected to the inner wall of the hanging ring; several experimental cages are hung side by side in each experimental space.

3. The anger modeling device based on combined emotional and physical stress according to claim 2, characterized in that: The top of the experimental cage is detachably and fixedly connected to a top cover, the front side wall of the experimental cage is provided with a cage door, the left and right side walls of the experimental cage are respectively penetrated and embedded with a food bottle and a water bottle, a glass bottom plate is fixedly provided at the lower part of the experimental cage, and an infrared camera is fixedly provided on the middle part of the upper surface of the bottom plate of the experimental cage below the glass bottom plate. The side walls of the experimental cage are made of non-light-transmitting material, and the glass bottom plate is made of germanium glass.

4. The anger modeling device based on combined emotional and physical stress according to claim 3, characterized in that: The top of the air inlet pipe in the same row of experimental cages is connected to the air supply pipe through a first flexible connecting pipe. One end of the air supply pipe is fixedly connected to the side wall of the experimental cage, and the other end passes through the outer wall of the experimental cage and is respectively connected to the cold air supply device and the hot air supply device through a first three-way joint. The first three-way joint is respectively provided with a first solenoid valve and a second solenoid valve for controlling the supply of cold air or hot air. The first solenoid valve, the second solenoid valve, the cold air supply device, and the hot air supply device are respectively electrically connected to the control system through wires.

5. The anger modeling device based on combined emotional and physical stress according to claim 4, characterized in that: The top of the water inlet pipe in the same row of experimental cages is connected to the water inlet main pipe through a second flexible connecting pipe. One end of the water inlet main pipe is fixedly connected to the side wall of the experimental cage, and the other end passes through the outer wall of the experimental cage and is connected to the cold water supply device and the hot water supply device respectively through the second three-way joint. The second three-way joint is respectively provided with a third solenoid valve and a fourth solenoid valve for controlling the supply of cold water or hot water. The third solenoid valve, the fourth solenoid valve, the cold water supply device, and the hot water supply device are respectively electrically connected to the control system through wires.

6. The anger modeling device based on combined emotional and physical stress according to claim 5, characterized in that: The top ends of the ventilation tubes in the same row are connected to a gas supply pipe through a third flexible connecting pipe. The third flexible connecting pipe is provided with a fifth solenoid valve. One end of the gas supply pipe is fixedly connected to the inner wall of the experimental cage, and the other end passes through the outer wall of the experimental cage and is connected to the stimulation gas supply device. The fifth solenoid valve and the stimulation gas supply device are electrically connected to the control system through wires.

7. The anger modeling device based on combined emotional and physical stress according to claim 6, characterized in that: The lower edge of the top cover is embedded in the top of the experimental cage and is detachably fixedly connected to the top of the experimental cage by positioning bolts. The left and right inner walls of the glass bottom plate are respectively provided with slopes. The side walls of the experimental cage located behind the glass bottom plate are respectively provided with protective nets for blocking experimental animals and allowing the experimental animal feces to pass through. A guide sleeve is fixedly provided on the rear wall surface of the experimental cage located behind the protective net. Damping guide rails are respectively provided on both sides of the bottom end of the experimental cage. A drawer with an open front end is inserted on the damping guide rail. The open end of the drawer is sealed and docked with the lower end of the guide cover and is used to collect feces and urine of the experimental animals.

8. The anger modeling device based on combined emotional and physical stress according to claim 7, characterized in that: The striking block is a cylindrical metal structure with a membrane pressure sensor provided on its outer periphery. The membrane pressure sensor is electrically connected to the control system via a wire and is used to detect the number and intensity of the experimental animal biting the striking block due to anger. The control system is provided with a timing module, which records the latency and duration of the experimental animal biting the striking block based on the visual information of the infrared camera. The latency refers to the time from the first time the experimental animal is hit to the first time it bites the striking block.

9. The anger modeling device based on combined emotional and physical stress according to claim 8, characterized in that: The control system is electrically connected to a human-computer interaction device via a wire. The human-computer interaction device is equipped with an operation interface. The operation interface is provided with an experimental condition input window, an experimental data display window, and an experimental result analysis display window. The experimental condition input window, the experimental data display window, and the experimental result analysis display window are respectively connected to the control module, storage module, and data analysis and processing module preset in the control system.

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