Composite plant extract with anti-inflammatory and antipruritic effects and method for preparing same
The compound plant extract prepared by combining Sichuan pepper fruit, pepper seeds, peony root, purslane, and sophora root with microbial inoculum treatment can quickly eliminate inflammation, significantly outperforming aspirin and achieving rapid anti-inflammatory and antipruritic effects.
Patent Information
- Application Number
- CN202310951134.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-31
- Publication Date
- 2025-12-26
- Estimated Expiration
- 2043-07-31
AI Technical Summary
Existing skincare products are generally not very effective at relieving inflammation and itching, and are difficult to quickly and effectively alleviate itching.
A compound plant extract was prepared by using extracts from Sichuan pepper fruit, pepper seeds, peony root, purslane, and sophora root, and by using steps such as soaking in microbial solution and freezing and heating to break down cell walls and fully extract active ingredients.
It achieves rapid elimination of inflammation, significantly outperforming aspirin, quickly relieving itching, and has better anti-inflammatory and antipruritic effects.
Smart Images
Figure CN116763696B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of cosmetics, in particular to a composite plant extract with anti-inflammatory and antipruritic effects and a preparation method thereof. BACKGROUND
[0002] Skin is the outermost organ of the human body, mainly to protect the human body, because the skin is in contact with the outside world, the skin will be hurt in various ways, when the skin is stimulated, will cause itching, especially under the stimulation, inflammatory reaction, inflammatory mediators further stimulate the "itch receptors", eventually form a strong itching, itching is very uncomfortable, scratching can cause further damage to the skin, so you need to apply skin care products with antipruritic effect to repair the skin, relieve the discomfort of the skin and promote the recovery of the skin.
[0003] The existing skin care products add substances with anti-inflammatory and antipruritic effects, so that the skin care products have anti-inflammatory and antipruritic effects, but the existing skin care products have general anti-inflammatory and antipruritic effects, and it is difficult to achieve rapid antipruritic effect, so there is room for improvement. SUMMARY
[0004] In order to improve the anti-inflammatory and antipruritic effects and achieve the effect of rapid antipruritic, the present application provides a composite plant extract with anti-inflammatory and antipruritic effects and a preparation method thereof.
[0005] In the first aspect, the present application provides a composite plant extract with anti-inflammatory and antipruritic effects, which adopts the following technical scheme:
[0006] A composite plant extract with anti-inflammatory and antipruritic effects is prepared by compounding and extracting Zanthoxylum bungeanum, Piper nigrum seeds, Paeonia suffruticosa, Portulaca oleracea and Sophora flavescens.
[0007] By adopting the above technical scheme, the composite plant extract prepared by compounding and extracting Zanthoxylum bungeanum, Piper nigrum seeds, Paeonia suffruticosa, Portulaca oleracea and Sophora flavescens can effectively eliminate inflammation, has better antipruritic effect, and has faster effect. The anti-inflammatory effect is obviously better than that of aspirin. By quickly eliminating inflammation, the inflammatory reaction is quickly eliminated, so as to cut off the stimulation source from the source, so as to make the itching disappear and achieve the effect of rapid anti-inflammatory and antipruritic.
[0008] Preferably, the mass ratio of Zanthoxylum bungeanum, Piper nigrum seeds, Paeonia suffruticosa, Portulaca oleracea and Sophora flavescens is 40-50:5-15:40-50:1-10:1-10.
[0009] By adopting the technical scheme, the active ingredients of the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are combined in a specific ratio to produce a better synergistic effect, eliminate inflammation more effectively, and take effect more quickly, thereby achieving a better and faster anti-inflammatory and itching-relieving effect.
[0010] Preferably, the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are all dried traditional Chinese medicinal materials.
[0011] By adopting the technical scheme, the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are all dried traditional Chinese medicinal materials, so that the active ingredients of the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are combined to achieve a better effect, thereby achieving a better and faster anti-inflammatory and itching-relieving effect.
[0012] In a second aspect, the application provides a preparation method of a compound plant extract with an anti-inflammatory and itching-relieving effect, which adopts the following technical scheme:
[0013] The preparation method of the compound plant extract with an anti-inflammatory and itching-relieving effect comprises the following steps:
[0014] Step 1), the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are soaked in water to obtain a pretreated compound;
[0015] Step 2), a compound microbial liquid is then added to the pretreated compound and soaked for 3-4 days to obtain a pre-extracted compound;
[0016] Step 3), the pre-extracted compound is frozen to below -10℃ for 2-3 hours, then thawed at room temperature and heated to boiling, and then condensed and refluxed for 4-6 hours of boiling extraction, filtered, and the filtrate is obtained;
[0017] Step 4), the filtrate is concentrated and dried to obtain the compound plant extract with an anti-inflammatory and itching-relieving effect.
[0018] By adopting the technical scheme, the cell walls of the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are well broken down by microbial decomposition, and the active ingredients in the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are fully extracted by freezing and using ice crystals to pierce the cell membranes, so that the active ingredients of the Zanthoxylum bungeanum fruit, Piper nigrum seed, Paeonia suffruticosa root, Portulaca oleracea, and Sophora flavescens root are combined to achieve a better effect, thereby achieving a better anti-inflammatory and itching-relieving effect.
[0019] Preferably, in step 2), the compound microbial liquid is activated in a culture solution after being compounded by Exidiopsis sp., Erwinia persicina, and Fusarium reticulatum.
[0020] By adopting the above technical solution and specifically selecting *Russula purpurea*, *Erwinia peachensis*, and *Fusarium solani*, the extraction effects of Sichuan pepper fruit, pepper seeds, peony root, purslane, and sophora flavescens root are improved, effectively reducing irritating substances and exhibiting better anti-inflammatory and antipruritic effects. Furthermore, the combination and activation of *Russula purpurea*, *Erwinia peachensis*, and *Fusarium solani* creates a better symbiotic system, thereby achieving a better cell wall decomposition effect in the combined system of Sichuan pepper fruit, pepper seeds, peony root, purslane, and sophora flavescens root, resulting in high extraction efficiency.
[0021] Preferably, the mass ratio of *Russula nigra*, *Erwinia peachensis*, and *Fusarium solani* is 1-2:1-2:1-2.
[0022] By adopting the above technical solution and specifically selecting the mass ratio of *Russula nigra*, *Erwinia peachensis*, and *Fusarium solani*, the extraction effect of Sichuan pepper fruit, pepper seeds, peony root, purslane, and sophora flavescens root is improved, and the quality of the compound plant extract is higher.
[0023] Preferably, the culture medium is made by juicing a mixture of apple, pear, bitter melon, tomato, cabbage, spinach, celery, and water.
[0024] By adopting the above technical solution, and specifically selecting a culture medium made by juicing a mixture of apples, pears, bitter melons, tomatoes, cabbage, spinach, celery, and water, *Russula nigra*, *Erwinia peachensis*, and *Fusarium solani* can better form a symbiotic relationship. *Russula nigra*, *Erwinia peachensis*, and *Fusarium solani* exhibit higher activity, resulting in better effects when used to simultaneously extract *Zanthoxylum bungeanum* fruit, pepper seeds, peony root, purslane, and *Sophora flavescens* root. The resulting compound plant extract has better anti-inflammatory and antipruritic effects.
[0025] Preferably, the mass ratio of apple, pear, bitter melon, tomato, cabbage, spinach, celery, and water is 10-12:5-7:3-5:7-9:6-8:2-4:4-6:100.
[0026] By adopting the above technical solution and specifically selecting the mass ratio of apples, pears, bitter melons, tomatoes, cabbage, spinach, celery, and water, the cultivation of *Russula purpurea*, *Erwinia peachensis*, and *Fusarium solani* is more effective, resulting in better extraction of Sichuan pepper fruit, pepper seeds, peony root, purslane, and sophora flavescens root by compound microorganisms. The resulting compound plant extract has better anti-inflammatory and antipruritic effects.
[0027] Preferably, in step 2), the OD value of the composite microbial solution is ≥1.8.
[0028] By adopting the technical scheme, the black ball paint spot bacteria, peach color Owen's bacteria and eroding pulse fusarium in the complex microbial bacteria liquid are in high activity after natural selection of the special liquid culture medium, the effect of decomposing and extracting the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens is better, and the prepared complex plant extract has better anti-inflammatory and itching relieving effects.
[0029] Preferably, in the step 2), the input mass of the complex microbial bacteria liquid is 1-2% of the total input mass of the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens in the step 1).
[0030] By adopting the technical scheme, the input amount of the complex microbial bacteria liquid is specifically selected, so that the decomposition and extraction effect is better, the cost can be better controlled, and the economic value is higher.
[0031] In summary, the present application has the following beneficial effects:
[0032] 1. The complex plant extract prepared by compounding and extracting the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens can effectively eliminate inflammation, has better itching relieving effect, takes effect faster, and has obvious anti-inflammatory effect better than aspirin. By quickly eliminating inflammation, the inflammatory reaction is quickly eliminated, so that the stimulation source is cut off from the source, and the itching sensation disappears, realizing the effect of rapid anti-inflammatory and itching relieving.
[0033] 2. In the present application, the black ball paint spot bacteria, peach color Owen's bacteria and eroding pulse fusarium are specifically selected, so that the extraction effect of the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens is better, the irritating substances are effectively reduced, the anti-inflammatory and itching relieving effects are better, and the black ball paint spot bacteria, peach color Owen's bacteria and eroding pulse fusarium are activated after compounding, so that a better symbiotic system is formed, thereby realizing the effect of better decomposition of cell walls in the compounding system of the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens, and having a good decomposition effect on the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens.
[0034] 3. In the present application, the culture liquid is prepared by mixing and juicing apples, pears, bitter melon, tomatoes, cabbage, spinach, celery and water, so that the black ball paint spot bacteria, peach color Owen's bacteria and eroding pulse fusarium can better form a symbiotic relationship, the activity of the black ball paint spot bacteria, peach color Owen's bacteria and eroding pulse fusarium is higher, the effect of simultaneously extracting the fruit of zanthoxylum bungeanum, pepper seed, peony root, purslane and sophora flavescens is better, and the prepared complex plant extract has better anti-inflammatory and itching relieving effects. BRIEF DESCRIPTION OF DRAWINGS
[0035] Figure 1 The figure is the efficacy experiment diagram of example 2 and aspirin. DETAILED DESCRIPTION
[0036] The application is further described in detail below in conjunction with examples.
[0037] Example 1
[0038] A composite plant extract with anti-inflammatory and antipruritic effects, and a method for preparing the composite plant extract with anti-inflammatory and antipruritic effects are as follows:
[0039] Step 1), weigh 40 kg of Zanthoxylum bungeanum fruits, 5 kg of Piper nigrum seeds, 40 kg of Paeonia lactiflora roots, 1 kg of Portulaca oleracea, and 1 kg of Sophora flavescens roots into a container, then add 348 kg of water and soak for 2 h to obtain a pretreated composite.
[0040] Step 2), then add 0.87 kg of composite microbial liquid to the pretreated composite and soak for 3 d to obtain a pre-extracted composite.
[0041] Step 3), freeze the pre-extracted composite to -10℃ for 2 h, then melt at room temperature and heat to boiling, condense and reflux, and maintain boiling extraction for 4 h, filter with 200 mesh filter cloth, and take the filtrate.
[0042] Step 4), rotary evaporate the filtrate to a paste, rotary evaporation pressure is 0.01 MPa, rotary evaporation temperature is 65℃, and the paste is spray dried to obtain a composite plant extract with anti-inflammatory and antipruritic effects.
[0043] The Zanthoxylum bungeanum fruits are commercially available and are sun-dried traditional Chinese medicinal materials.
[0044] The Piper nigrum seeds are commercially available and are sun-dried traditional Chinese medicinal materials.
[0045] The Paeonia lactiflora roots are commercially available and are sun-dried traditional Chinese medicinal materials.
[0046] The Portulaca oleracea is commercially available and is sun-dried traditional Chinese medicinal materials.
[0047] The Sophora flavescens roots are commercially available and are sun-dried traditional Chinese medicinal materials.
[0048] The composite microbial liquid is self-made, and the preparation method of the composite microbial liquid is as follows:
[0049] Step 01), weigh 1 kg of apples, 0.5 kg of pears, 0.6 kg of bitter gourds, 0.7 kg of tomatoes, 0.6 kg of cabbage, 0.2 kg of spinach, 0.4 kg of celery, and 10 kg of water into a homogenizer to form a composite slurry.
[0050] Step 02), filter the composite slurry through a 200 mesh filter cloth to obtain a culture solution.
[0051] Step 03), inoculate 10 mg of Guignardia bidwellii, 10 mg of Erwinia persicina, and 10 mg of Fusarium reticulatum in the culture solution, incubate at 36℃ until the OD value is greater than or equal to 1.8, and obtain a composite microbial solution.
[0052] In the formula, the apples are commercially available and are freshly picked.
[0053] In the formula, the snow pears are commercially available and are freshly picked.
[0054] In the formula, the bitter gourds are commercially available and are freshly picked.
[0055] In the formula, the tomatoes are commercially available and are freshly picked.
[0056] In the formula, the cabbages are commercially available and are freshly picked.
[0057] In the formula, the spinach is commercially available and is freshly picked.
[0058] In the formula, the celery is commercially available and is freshly picked.
[0059] In the formula, the Guignardia bidwellii is purchased from the Shanghai Center for Bio-Organic Technology, and the strain number is SHBCC D81894.
[0060] In the formula, the Erwinia persicina is purchased from the Shanghai Center for Bio-Organic Technology, and the strain number is SHBCC D80291.
[0061] In the formula, the Fusarium reticulatum is purchased from the Shanghai Center for Bio-Organic Technology, and the strain number is SHBCC D81870.
[0062] Example 2
[0063] A composite plant extract with anti-inflammatory and itching-relieving effects, and a preparation method of the composite plant extract with anti-inflammatory and itching-relieving effects are as follows:
[0064] Step 1), weigh 45 kg of Zanthoxylum bungeanum fruits, 10 kg of Piper nigrum seeds, 45 kg of Paeonia suffruticosa roots, 5 kg of Portulaca oleracea, and 5 kg of Sophora flavescens roots into a container, then add 440 kg of water, soak for 2 h, and obtain a pretreated composite.
[0065] Step 2), then add 1.65 kg of a composite microbial solution to the pretreated composite, soak for 3-4 d, and obtain a pre-extracted composite.
[0066] Step 3), freeze the pre-extracted composite to -10℃ for 2-3 h, melt at room temperature, heat to boiling, condense reflux, maintain boiling extraction for 4-6 h, filter through 200 mesh filter cloth, and take the filtrate.
[0067] Step 4), the filtrate is concentrated to a paste by rotary evaporation at a rotary evaporation pressure of 0.01 MPa and a rotary evaporation temperature of 65 DEG C, and the paste is spray-dried to obtain the compound plant extract having anti-inflammatory and itching-relieving effects.
[0068] The Zanthoxylum bungeanum is commercially available and is a dried traditional Chinese medicinal material.
[0069] The Piper nigrum is commercially available and is a dried traditional Chinese medicinal material.
[0070] The Paeonia lactiflora is commercially available and is a dried traditional Chinese medicinal material.
[0071] The Portulaca oleracea is commercially available and is a dried traditional Chinese medicinal material.
[0072] The Sophora flavescens is commercially available and is a dried traditional Chinese medicinal material.
[0073] The compound microbial liquid is self-prepared, and the preparation method of the compound microbial liquid is as follows:
[0074] Step 01), 1.1 kg of apples, 0.6 kg of pears, 0.4 kg of bitter gourds, 0.8 kg of tomatoes, 0.7 kg of cabbages, 0.3 kg of spinach, 0.5 kg of celery, and 10 kg of water are weighed and put into a homogenizer to form a compound slurry.
[0075] Step 02), the compound slurry is filtered through a 200-mesh filter cloth to obtain a culture solution.
[0076] Step 03), 10 mg of Botryobasidium pullulans, 20 mg of Erwinia carotovora, and 10 mg of Fusarium reticulatum are inoculated into the culture solution, and the temperature is kept at 36 DEG C until the OD value is greater than or equal to 1.8 to obtain a compound microbial liquid.
[0077] The apples are commercially available and are freshly picked.
[0078] The pears are commercially available and are freshly picked.
[0079] The bitter gourds are commercially available and are freshly picked.
[0080] The tomatoes are commercially available and are freshly picked.
[0081] The cabbages are commercially available and are freshly picked.
[0082] The spinach is commercially available and is freshly picked.
[0083] The celery is commercially available and is freshly picked.
[0084] The Botryobasidium pullulans is purchased from the Shanghai Collection Center of Biological Technology, and the strain number is SHBCC D81894.
[0085] Among them, the Erwinia persicina is purchased from Shanghai Center for Biological and Biological Technology Preservation, and the strain number is SHBCC D80291.
[0086] Among them, the Erwinia persicina is purchased from Shanghai Center for Biological and Biological Technology Preservation, and the strain number is SHBCC D80291.
[0087] Example 3
[0088] A composite plant extract with anti-inflammatory and antipruritic effects, and a preparation method of the composite plant extract with anti-inflammatory and antipruritic effects are as follows:
[0089] Step 1), weigh 50 kg of Zanthoxylum bungeanum fruits, 15 kg of Piper nigrum seeds, 50 kg of Paeonia suffruticosa roots, 10 kg of Portulaca oleracea, and 10 kg of Sophora flavescens roots into a container, then add 540 kg of water and soak for 2 h to obtain a pretreated composite.
[0090] Step 2), then add 2.7 kg of composite microbial liquid to the pretreated composite and soak for 3-4 d to obtain a pre-extracted composite.
[0091] Step 3), freeze the pre-extracted composite to -10℃ for 2-3 h, melt at room temperature, then heat to boiling, condense reflux, and keep boiling for 4-6 h, filter with 200 mesh filter cloth, and take the filtrate.
[0092] Step 4), rotary evaporate the filtrate to paste, rotary evaporate at 0.01 MPa and 65℃, spray dry the paste to obtain a composite plant extract with anti-inflammatory and antipruritic effects.
[0093] Among them, the Zanthoxylum bungeanum fruits are commercially available and are dried traditional Chinese medicinal materials.
[0094] Among them, the Piper nigrum seeds are commercially available and are dried traditional Chinese medicinal materials.
[0095] Among them, the Paeonia suffruticosa roots are commercially available and are dried traditional Chinese medicinal materials.
[0096] Among them, the Portulaca oleracea is commercially available and is a dried traditional Chinese medicinal material.
[0097] Among them, the Sophora flavescens roots are commercially available and are dried traditional Chinese medicinal materials.
[0098] Among them, the composite microbial liquid is self-made, and the preparation method of the composite microbial liquid is as follows:
[0099] Step 01), weigh 1.2 kg of apples, 0.7 kg of pears, 0.5 kg of bitter gourd, 0.9 kg of tomatoes, 0.8 kg of cabbage, 0.4 kg of spinach, 0.6 kg of celery, and 10 kg of water into a homogenizer to form a composite slurry.
[0100] Step 02), the composite slurry was filtered by 200 mesh filter cloth to obtain the culture solution.
[0101] Step 03), 20 mg of Guignardia bidwellii, 10 mg of Erwinia persicina and 20 mg of Fusarium reticulatum were inoculated in the culture solution, and incubated at 36°C until the OD value was greater than or equal to 1.8 to obtain the composite microbial solution.
[0102] Among them, apples are commercially available and freshly picked.
[0103] Among them, snow pears are commercially available and freshly picked.
[0104] Among them, bitter gourds are commercially available and freshly picked.
[0105] Among them, tomatoes are commercially available and freshly picked.
[0106] Among them, cabbages are commercially available and freshly picked.
[0107] Among them, spinach is commercially available and freshly picked.
[0108] Among them, celery is commercially available and freshly picked.
[0109] Among them, Guignardia bidwellii is purchased from Shanghai Collection Center of Biological Technology, and the strain number is SHBCC D81894.
[0110] Among them, Erwinia persicina is purchased from Shanghai Collection Center of Biological Technology, and the strain number is SHBCC D80291.
[0111] Among them, Fusarium reticulatum is purchased from Shanghai Collection Center of Biological Technology, and the strain number is SHBCC D81870.
[0112] Comparative Example 1
[0113] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, only differs in that:
[0114] Chrysanthemum morifolium is used to replace Zanthoxylum bungeanum in an equal amount.
[0115] Among them, Chrysanthemum morifolium is commercially available and is a dried traditional Chinese medicine material.
[0116] Comparative Example 2
[0117] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, only differs in that:
[0118] Lonicera japonica is used to replace Zanthoxylum bungeanum seeds in an equal amount.
[0119] Among them, Lonicera japonica is commercially available and is a dried traditional Chinese medicine material.
[0120] Comparative Example 3
[0121] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0122] Adopting equal amount of Radix Paeoniae Alba to replace Radix Paeoniae Rubra.
[0123] Among them, Radix Paeoniae Alba is from the market, and is a dried traditional Chinese medicinal material.
[0124] Comparative Example 4
[0125] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0126] Among them, Radix Paeoniae Alba is from the market, and is a dried traditional Chinese medicinal material.
[0127] Comparative Example 5
[0128] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0129] Among them, Radix Paeoniae Alba is from the market, and is a dried traditional Chinese medicinal material.
[0130] Comparative Example 6
[0131] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0132] Among them, Radix Paeoniae Alba is from the market, and is a dried traditional Chinese medicinal material.
[0133] Comparative Example 7
[0134] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0135] Among them, Radix Paeoniae Alba is from the market, and is a dried traditional Chinese medicinal material.
[0136] Comparative Example 8
[0137] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0138] Among them, Radix Paeoniae Alba is from the market, and is a dried traditional Chinese medicinal material.
[0139] Comparative Example 9
[0140] A composite plant extract with anti-inflammatory and itching-relieving effects, compared with Example 2, the only difference is that:
[0141] Wherein, the grape is from the market, and is freshly picked.
[0142] Comparative Example 10
[0143] A compound plant extract with anti-inflammatory and antipruritic effects, compared with Example 2, the only difference is that the kale is used to replace the cabbage.
[0144] Wherein, the kale is from the market, and is freshly picked.
[0145] Comparative Example 11
[0146] A compound plant extract with anti-inflammatory and antipruritic effects, compared with Example 2, the only difference is that the lettuce is used to replace the spinach.
[0147] Wherein, the lettuce is from the market, and is freshly picked.
[0148] Comparative Example 12
[0149] A compound plant extract with anti-inflammatory and antipruritic effects, compared with Example 2, the only difference is that:
[0150] The cumin is used to replace the celery.
[0151] Wherein, the cumin is from the market, and is freshly picked.
[0152] Comparative Example 13
[0153] A compound plant extract with anti-inflammatory and antipruritic effects, compared with Example 2, the only difference is that:
[0154] The Trichoderma viride is used to replace the Exidiopsis pythioides.
[0155] Wherein, the Trichoderma viride is purchased from Shanghai Cryobank Center, and the strain number is SHBCC D24879.
[0156] Comparative Example 14
[0157] A compound plant extract with anti-inflammatory and antipruritic effects, compared with Example 2, the only difference is that:
[0158] The Bacillus subtilis is used to replace the Erwinia persicina.
[0159] Wherein, the Bacillus subtilis is purchased from Shanghai Cryobank Center, and the strain number is SHBCC D50760.
[0160] Comparative Example 15
[0161] A compound plant extract with anti-inflammatory and antipruritic effects, compared with Example 2, the only difference is that:
[0162] Aspergillus niger was used to replace the same amount of Fusarium reticulatum.
[0163] Aspergillus niger was purchased from Shanghai Collection Center of Biological Technology, and the strain number was SHBCC D19043.
[0164] Experiment 1
[0165] The antipruritic effects of each example, the comparative example, and aspirin were detected.
[0166] 1. Method name: Zebrafish antipruritic effect test method.
[0167] 2. System and sample size:
[0168] 2.1 Test system: Transgenic neutrophil green fluorescent zebrafish (MPX).
[0169] 2.2 Zebrafish age: 3 days after fertilization (3dpf).
[0170] 2.3 Sample size per experimental group: 15 (N = 10).
[0171] 2.4 Fish rearing and breeding method: According to the company's laboratory standard rearing and breeding method, it meets the requirements of international AAALAC certification (certification number: 001458).
[0172] 3. Principle method:
[0173] Itching usually includes different degrees of burning, pricking, and pain. Skin itching is common in dry skin, allergies, mosquito bites, and various inflammatory or non-inflammatory skin diseases. By injecting LPS into the yolk sac of zebrafish to establish an inflammation model, zebrafish develop an inflammatory response, neutrophils develop an immune response, and the neutrophils of transgenic neutrophil fluorescent zebrafish can be clearly observed under a fluorescence microscope. Therefore, the anti-inflammatory effect of the sample can be evaluated by observing the reduction in the number of neutrophils in the yolk sac of zebrafish, thereby determining whether the sample has an antipruritic effect.
[0174] 4. Experimental steps:
[0175] S1, randomly select zebrafish in a 6-well plate, 15 per well.
[0176] S2, give the sample (each example, the comparative example, aspirin, concentration 0.01%) in water, at the same time set up normal control group and model control group, each well capacity is 3mL.
[0177] S3, incubate at 28℃ in the dark for 1h.
[0178] S4, after incubation, inject an equal amount of LPS to establish a zebrafish skin inflammation model.
[0179] S5, continue to incubate for 2h under the condition of 28℃ in the dark.
[0180] S6, randomly select 10 zebra fish in each experimental group and place them under the fluorescence microscope to take photos, analyze and collect data by using image processing software, analyze the number of neutrophils (N) in the yolk sac of zebra fish, calculate the antipruritic efficacy of the sample according to the following formula, and determine whether it has antipruritic efficacy.
[0181]
[0182] 5. Judgment basis:
[0183] Significant difference is determined when p<0.05.
[0184] The specific detection data of experiment 1 is shown in table 1.
[0185] The efficacy experiment graph of example 2 and aspirin is shown in Figure 1 .
[0186] Figure 1 In the table, 1 is the normal control group, 2 is the model control group, 3 is aspirin (concentration 0.01%), 4 is the complex plant extract with anti-inflammatory and antipruritic efficacy of example 2 (concentration 0.01%), and the dashed part is the quantitative area.
[0187] Table 1
[0188]
[0189]
[0190] According to the data comparison in table 1, the complex plant extract prepared by compounding and extracting from zanthoxylum bungeanum fruit, pepper seed, paeonia lactiflora root, portulaca oleracea, and sophora flavescens root has good anti-inflammatory and antipruritic efficacy, and its efficacy is much higher than that of aspirin, which can achieve the effect of quickly relieving inflammation and itching.
[0191] The complex plant extract prepared by compounding and extracting from the complex of zanthoxylum bungeanum fruit, pepper seed, paeonia lactiflora root, portulaca oleracea, and sophora flavescens root using phaeosphaeria nodorum, erwinia persicina, and fusarium reticulatum can further improve the anti-inflammatory and antipruritic efficacy, and can more quickly achieve the effect of relieving inflammation and itching.
[0192] Activating the complex bacteria of phaeosphaeria nodorum, erwinia persicina, and fusarium reticulatum using a culture solution prepared by mixing and juicing apples, snow pears, bitter melons, tomatoes, cabbage, spinach, celery, and water can form a better symbiotic system through natural selection, so that the effect of extracting the complex of zanthoxylum bungeanum fruit, pepper seed, paeonia lactiflora root, portulaca oleracea, and sophora flavescens root is better, and the anti-inflammatory and antipruritic efficacy of the prepared complex plant extract is better, which can more quickly achieve the effect of relieving inflammation and itching.
[0193] The embodiments are only illustrative of the present application, and are not intended to limit the present application, and those skilled in the art can make modifications to the embodiments without creative contribution after reading the specification, but as long as the modifications are within the scope of the claims of the present application, they are protected by the patent law.
Claims
1. A composite plant extract having anti-inflammatory and antipruritic effects, characterized by: The compound plant extract with anti-inflammatory and itching-relieving effects is prepared from Zanthoxylum bungeanum Maxim, Piper nigrum L, Paeonia suffruticosa Andr, Portulaca oleracea L and Sophora flavescens Ait. The mass ratio of Zanthoxylum bungeanum Maxim, Piper nigrum L, Paeonia suffruticosa Andr, Portulaca oleracea L and Sophora flavescens Ait is 40-50:5-15:40-50:1-10:1-10. The preparation method of the compound plant extract with anti-inflammatory and itching-relieving effects comprises the following steps: Step 1), Zanthoxylum bungeanum Maxim, Piper nigrum L, Paeonia suffruticosa Andr, Portulaca oleracea L and Sophora flavescens Ait are put into water for soaking to obtain a pretreated compound; Step 2), then, a compound microbial liquid is put into the pretreated compound for soaking for 3-4 days to obtain a pre-extracted compound; Step 3), the pre-extracted compound is frozen to below-10 DEG C for 2-3 hours, then is melted at room temperature, and then is heated to boiling, condensed and refluxed, and kept boiling for 4-6 hours, and then is filtered to obtain a filtrate; Step 4), the filtrate is concentrated and dried to obtain the compound plant extract with anti-inflammatory and itching-relieving effects; In step 2), the compound microbial liquid is activated in a culture solution after compounding of the black ball paint spot fungus, the peach color Erwinia and the eroded pulse fusarium; The mass ratio of the black ball paint spot fungus, the peach color Erwinia and the eroded pulse fusarium is 1-2:1-2:1-2; The culture solution is obtained by mixing and juicing apples, snow pears, balsams, tomatoes, cabbages, spinach, celery and water; The mass ratio of the apples, snow pears, balsams, tomatoes, cabbages, spinach, celery and water is 10-12:5-7:3-5:7-9:6-8:2-4:4-6:
100.
2. The compound plant extract having anti-inflammatory and antipruritic effects according to claim 1, characterized by: The Zanthoxylum bungeanum Maxim, Piper nigrum L, Paeonia suffruticosa Andr, Portulaca oleracea L and Sophora flavescens Ait are all dried traditional Chinese medicinal materials.
3. The compound plant extract having anti-inflammatory and antipruritic effects according to claim 1, characterized by: In step 2), the OD value of the compound microbial liquid is greater than or equal to 1.
8.
4. The compound plant extract having anti-inflammatory and antipruritic effects according to claim 1, characterized by: In step 2), the input mass of the compound microbial liquid is 1-2% of the total input mass of Zanthoxylum bungeanum Maxim, Piper nigrum L, Paeonia suffruticosa Andr, Portulaca oleracea L and Sophora flavescens Ait in step 1).
Citation Information
Patent Citations
Moisturizing, soothing, itching-relieving and skin-care cream and preparation method thereof
CN113197836A