A high-efficiency histidine-rich protein separation chromatographic material P(NIPAM-co-AAc)-PAMAM@Cu and a preparation method thereof
By utilizing P(NIPAM-co-AAc)-PAMAM@Cu material, the problems of insufficient capacity and selectivity of histidine-rich protein separation media in existing technologies are solved by leveraging the amphiphilic gel and the specificity of metal ligands, thus achieving highly efficient protein drug separation.
CN116789974BActive Publication Date: 2026-05-29ZHENGZHOU UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHENGZHOU UNIV
- Filing Date
- 2023-06-13
- Publication Date
- 2026-05-29
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Abstract
The application discloses a kind of high-efficiency histidine-rich protein separation chromatography materials P (NIPAM-co-AAc)-PAMAM@Cu and preparation method thereof, belong to functional polymer chromatography material technical field;Its preparation method includes the following steps: with azobisdimethyl isobutyronitrile (AIBN) as initiator, N,N-methylene bisacrylamide (MBA) is used as crosslinking agent, N-isopropyl acrylamide (NIPAM) and acrylic acid (AAc) are used as monomer, polyamidoamine (PAMAM) is used as function group, prepare P (NIPAM-co-AAc)-PAMAM hydrogel, after chelation with copper nitrate, finally the chromatography material P (NIPAM-co-AAc)-PAMAM@Cu is prepared.The chromatography material prepared by the application, under certain conditions, the equilibrium adsorption capacity of BHb, BSA, Lyz is 646.23mg / g, 108.33mg / g and 56.24mg / g respectively, has the advantages of large adsorption capacity and high selectivity for histidine-rich protein;And the chromatography material has good separation performance and cyclic regeneration, provides a new way for the separation and purification of histidine-rich protein.
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