A device and method for the treatment and preservation of umbilical cord mesenchymal stem cells

Abstract

The application discloses a kind of umbilical cord mesenchymal stem cell processing and storing device and method, it is related to cell storage technical field.The umbilical cord mesenchymal stem cell processing and storing device, by setting storage tube, injection tube, refrigerator and wide-angle camera, umbilical cord mesenchymal stem cell is stored into storage tube, by wide-angle camera can be monitored in real time, when needing to replace new storage liquid, injection tube is inserted into storage tube and old storage liquid is extracted, replace new storage liquid, with the effect that storage tube does not need to be taken out to replace storage liquid, internal cold air leakage can be prevented, the common storage processing device can be realized to the freezing and short-term storage treatment of umbilical cord mesenchymal stem cell, umbilical cord mesenchymal stem cell needs to replace new storage liquid in the process of storage treatment, but most of prior art needs to be taken out to replace storage liquid, there is the problem of inconvenient operation.

Description

Technical Field

[0001] This invention relates to the field of cell preservation technology, specifically to a device and method for processing and preserving umbilical cord mesenchymal stem cells. Background Technology

[0002] Umbilical cord mesenchymal stem cells (MSCs) are pluripotent stem cells found in the umbilical cord tissue of newborns. They can differentiate into many types of tissue cells and have broad clinical application prospects. MSCs possess high differentiation potential and can differentiate in multiple directions. They show great clinical application potential in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium, and myocardium. Reports indicate that MSCs can be isolated from human umbilical cords, with superior cell content and proliferation capacity compared to bone marrow MSCs. They also exhibit lower immunogenicity and advantages such as convenient sourcing and lack of ethical controversy, thus attracting increasing attention from researchers.

[0003] Chinese utility model patent CN207355339U discloses a device for preserving umbilical cord mesenchymal stem cells. A first insulation ring is circumferentially fixed to the side of a first preservation box, and a second insulation ring is circumferentially fixed to the side of a second preservation box. Cooling fluid can be injected into the box to maintain its temperature, preventing rapid internal heating and cell damage. A cooling device can be installed in the water tank to ensure the coolant temperature remains constant during circulation. Both the first and second boxes have insulation and dustproof functions. The double-layered design provides better insulation, greater safety and reliability, and ease of movement.

[0004] The invention patent with publication number CN114916536B discloses a device for preserving umbilical cord mesenchymal stem cells. In the cryopreservation unit, the cryopreservation holes are arranged radially, with each radial radius corresponding to a cryopreservation buffer shelf. Each radial radius also corresponds to an infrared emitter. The emitted signal from the infrared emitter is received by an infrared receiver, which can quickly determine the cryopreservation buffer shelf that needs to be retrieved. Each time, only one cryopreservation hole is opened, reducing the diameter of the opening where liquid nitrogen comes into contact with the outside environment, thereby reducing the loss of liquid nitrogen. In the magnetic automatic retrieval mechanism, the magnetic automatic retrieval mechanism corresponds one-to-one with the cryopreservation buffer shelf. The control panel is used to determine the location of the cryopreservation buffer shelf to be found, and the retrieval electromagnet is automatically activated to attract the cryopreservation buffer shelf. A buffer block is set at the upper end of the cryopreservation buffer shelf, which reduces the impact force on the cryopreservation plate during the attraction process, avoiding damage to the stem cell cryopreservation tube due to impact. Furthermore, during the retrieval process, the staff does not need to put their hands into the device, reducing the risk of frostbite to the staff.

[0005] Common preservation devices can perform freezing and short-term preservation of umbilical cord mesenchymal stem cells. During the preservation process, it is necessary to replace the preservation solution. However, most existing technologies require removing the container to replace the preservation solution, which is inconvenient. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a device and method for processing and preserving umbilical cord mesenchymal stem cells. This solves the problem that while common preservation devices can achieve freezing and short-term preservation of umbilical cord mesenchymal stem cells, the preservation process requires changing the preservation solution. However, existing technologies generally require removing the container to change the preservation solution, which is inconvenient.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a device for processing and preserving umbilical cord mesenchymal stem cells, comprising a preservation box, wherein a plurality of placement holes are symmetrically formed on the surface of the preservation box, and a plurality of storage tubes for preserving umbilical cord mesenchymal stem cells are placed in the preservation box through the placement holes; a connecting cap for sealing is threaded onto the inner sidewall of the placement holes; a first placement groove and a second placement groove are also formed on the surface of the preservation box, wherein a syringe and an injection tube are respectively placed inside the first placement groove and the second placement groove; the injection tube can be connected to the end of the syringe and inserted into the inside of the storage tube to replace the preservation solution inside the storage tube; a cooler is fixedly connected to the bottom of the inner sidewall of the preservation box, a wide-angle camera is fixedly connected to the inner sidewall of the preservation box, and a carrying component for carrying the preservation box is provided on the top of the preservation box.

[0008] Further, the storage tube includes a receiving tube, an upper seal, and a lower seal; the upper seal is sealed on the top of the receiving tube, and the upper seal includes a peripheral portion, an inner connection portion, and a puncture portion. The peripheral portion, the inner connection portion, and the puncture portion are integrally formed. An annular cavity adapted to the wall thickness of the receiving tube is formed between the peripheral portion and the inner connection portion so that the inner sidewall of the peripheral portion and the outer sidewall of the inner connection portion are movably connected to the outer sidewall and the inner sidewall of the receiving tube, respectively. The puncture portion is fixed on the inner sidewall of the inner connection portion. An insertion hole for the injection tube to pass through is provided at the top center of the puncture portion. A rubber stopper is provided between the inner connection portion and the puncture portion, and the puncture portion pierces the interior of the rubber stopper. The lower seal is sealed on the bottom of the receiving tube.

[0009] Furthermore, annular protrusions are integrally formed on the outer wall of the accommodating tube at positions corresponding to the upper and lower seals, and the two annular protrusions are movably connected to the inner wall of the outer periphery and the inner wall of the lower seal, respectively.

[0010] Furthermore, the lower seal includes an outer part and an injection part with an opening. A magnetic ring is fixedly connected to the inner wall of the outer part, and the inner wall of the magnetic ring is fixedly connected to the surface of the injection part. A T-shaped rod is fixedly connected to the top of the injection part, and a retaining spring is sleeved on the surface of the T-shaped rod. One end of the retaining spring is fixedly connected to the surface of the magnetic guide plate, and the magnetic guide plate is movably sleeved on the surface of the T-shaped rod. The bottom of the storage box is provided with an injection assembly for extracting the storage solution. It is assembled such that when the storage tube is stored inside the storage box, the magnetic guide plate disengages from the magnetic ring so that the injection tube extracts the old storage solution while the new storage solution is injected.

[0011] Furthermore, the injection assembly includes an injection tube, a control valve, and an injection pump. The control valve is fixedly connected to the bottom of the storage box, the injection pump is fixedly connected to the outlet of the control valve, and the injection tube is fixedly connected to the inlet of the control valve, passes through the storage box, and is inserted into the interior of the lower seal.

[0012] Furthermore, a positioning element is movably sleeved on the surface of the lower seal, and a guide rod is connected to the surface of the positioning element. One end of the guide rod movably passes through the positioning element and is fixedly connected to the bottom of the inner sidewall of the storage box, while the other end of the guide rod is fixedly connected to the top of the inner sidewall of the storage box. A return spring is sleeved on the surface of the guide rod, with one end of the return spring fixedly connected to the surface of the positioning element and the other end fixedly connected to the top of the inner sidewall of the storage box. A first rubber ring is fixedly sleeved on the surface of the positioning element, and a second rubber ring is fixedly sleeved on the surface of the upper seal. The surface of the second rubber ring is movably connected to the inner sidewall of the storage box. A positioning component for limiting the position of the storage tube is provided at the placement hole on the storage box.

[0013] Furthermore, the positioning component includes a positioning block, a positioning spring, and a positioning plate. The surface of the positioning block is fixedly connected to one end of the positioning spring, and the other end of the positioning spring is fixedly connected to the inner wall of the placement hole. The positioning plate is used to limit the positioning block.

[0014] Furthermore, the carrying assembly includes a telescopic rod and a carrying tube. Two telescopic rods are movably connected to the top of the storage box. One end of the telescopic rod is fixedly connected to a sealing cap, and the other end of the telescopic rod is fixedly connected to a fixing block. A threaded ring is movably fitted onto the surface of the fixing block. One end of the carrying tube is movably connected to the sealing cap, and the other end of the carrying tube is threadedly connected to the threaded ring. A threaded rod is connected to the inside of the carrying tube via a drive motor. A lifting component is threadedly fitted onto the surface of the threaded rod. A limiting block connected to the inner wall of the carrying tube is provided on the surface of the lifting component. A surface fixing component for installing an injection tube is also connected to the surface of the lifting component.

[0015] Furthermore, the fixing member includes an annular portion and a fastening portion. The annular portion has an internal thread, and the injection tube has an external thread. The fastening portion is fixedly connected to the surface of the annular portion, and the surface of the fastening portion engages with a slot on the lifting member. A spring is fixedly connected to the surface of the fastening portion, and one end of the spring is fixedly connected to the surface of the annular portion. A magnet is fixedly connected to the bottom of the annular portion. The magnet can press the positioning block to detach it from the top surface of the upper sealing member. The upper sealing member is made of a magnetic material, and the surface of the carrying tube has a notch.

[0016] A method for processing and preserving umbilical cord mesenchymal stem cells, using the aforementioned umbilical cord mesenchymal stem cell processing and preservation device, includes the following steps:

[0017] S1. Ensure that the storage tubes and other components inside the storage box are clean and sterile, and prepare the storage solution to ensure proper storage conditions;

[0018] S2. Collect umbilical cord mesenchymal stem cells. During the surgery or collection process, collect the umbilical cord mesenchymal stem cells into a suitable container or collector.

[0019] S3. Transfer the collected umbilical cord mesenchymal stem cells to the storage tube, open the connecting cover on the storage box, insert the storage tube into the placement hole of the storage box, ensure that the connecting cover is tightly sealed, and the bottom of the storage box is fixedly connected to a cooler to ensure that the inside of the storage box maintains a suitable temperature to prolong the survival period of the stem cells.

[0020] S4. During the preservation process, the preservation solution needs to be changed regularly to maintain the excellent condition of the stem cells. Open the first placement slot on the preservation box, take out the syringe, connect the injection tube, insert the injection tube into the storage tube, draw out the old preservation solution from the storage tube, and inject the preservation solution using the injection component at the bottom of the preservation box.

[0021] S5. Using a wide-angle camera fixedly connected to the preservation box, the status of stem cells in the preservation box can be monitored, and relevant information of stem cells in the preservation box, such as preservation time and preservation solution replacement records, can be recorded regularly.

[0022] S6. When stem cells are needed, remove them from the storage tube for further processing or application.

[0023] The present invention has the following beneficial effects:

[0024] (1) The device for processing and preserving umbilical cord mesenchymal stem cells, by setting up a storage tube, an injection tube, a cooler and a wide-angle camera, preserves umbilical cord mesenchymal stem cells in the storage tube. The wide-angle camera can monitor in real time. When it is necessary to replace the preservation solution, the injection tube is inserted into the storage tube to extract the old preservation solution and then replace it with a new one. It has the effect of replacing the preservation solution without removing the storage tube, and at the same time, it can prevent the leakage of cold air inside. It solves the problem that common preservation and processing devices can realize the freezing and short-term preservation of umbilical cord mesenchymal stem cells. However, in the process of preserving umbilical cord mesenchymal stem cells, it is necessary to replace the preservation solution. But most existing technologies require the container to be removed to replace the preservation solution, which is inconvenient to operate.

[0025] (2) The device for processing and preserving umbilical cord mesenchymal stem cells is designed with an upper seal, a lower seal, and a receiving tube to facilitate the assembly of the storage tube. The structure of the lower seal facilitates the insertion of the injection tube, while the magnetic plate and magnetic ring inside the lower seal enable the injection component to inject new preservation solution, making it easy to replace the preservation solution.

[0026] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 This is a schematic diagram of the bottom structure of the storage box of the present invention;

[0029] Figure 3 This is a schematic diagram of the internal structure of the storage box of the present invention;

[0030] Figure 4 This is a schematic diagram of the structure of the refrigerator in this invention;

[0031] Figure 5 This is a schematic diagram of the structure of the storage tube in this invention;

[0032] Figure 6 This is a front view of the storage tube of the present invention;

[0033] Figure 7 For the present invention Figure 6 Sectional view along line AA;

[0034] Figure 8 This is a schematic diagram of the positioning component structure of the present invention;

[0035] Figure 9 This is a schematic diagram of the magnetic conductive plate structure of the present invention;

[0036] Figure 10 This is a schematic diagram of the lower sealing component structure of the present invention;

[0037] Figure 11 This is a schematic diagram of the upper sealing structure of the present invention;

[0038] Figure 12 This is a schematic diagram of the internal structure of the carrying tube of the present invention;

[0039] Figure 13 This is a schematic diagram of the bottom structure of the carrying tube of the present invention;

[0040] Figure 14 This is a top view of the carrying tube of the present invention;

[0041] Figure 15 For the present invention Figure 14 Sectional view along line BB;

[0042] Figure 16 This is a schematic diagram of the lifting component of the present invention;

[0043] Figure 17 This is a schematic diagram of the lifting component structure of the present invention;

[0044] Figure 18 This is a schematic diagram of the fastener structure of the present invention;

[0045] Figure 19 This is a schematic diagram of the injection tube structure of the present invention;

[0046] Figure 20 This is a schematic diagram of the structure at the fixing block of the present invention;

[0047] Figure 21 This is a flowchart of the method for processing and preserving umbilical cord mesenchymal stem cells according to the present invention.

[0048] In the diagram, 1. Storage box; 2. Placement hole; 3. Storage tube; 301. Receptacle tube; 302. Upper seal; 3021. Outer perimeter; 3022. Inner connection; 3023. Puncture section; 303. Lower seal; 3031. Outer connection; 3032. Injection section; 4. Connecting cap; 5. First placement slot; 6. Second placement slot; 7. Syringe; 8. Injection tube; 9. Cooler; 10. Wide-angle camera; 11. Puncture hole; 12. Rubber stopper; 13. Annular protrusion; 14. Magnetic ring; 15. T-shaped rod; 16. Abutment spring; 17. Magnetic guide plate; 18. Notch; 19. Injection tube; 20. Control valve; 21. Injection pump; 22. Positioning component; 23. Guide rod; 24. Return spring; 25. First rubber ring; 26. Second rubber ring; 27. Positioning block; 28. Positioning spring; 29. ​​Positioning plate; 30. Telescopic rod; 31. Lifting tube; 32. Sealing cap; 33. Fixing block; 34. Threaded ring; 35. Threaded rod; 36. Lifting component; 37. Limiting block; 38. Fixing component; 3801. Annular part; 3802. Fastening part; 39. Internal thread; 40. External thread; 41. Slot; 42. Spring piece; 43. Magnet block. Detailed Implementation

[0049] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0050] In the description of this invention, it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inner", "around", etc., which indicate orientation or positional relationship, are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as limiting this invention.

[0051] Please see Figures 1-20This invention provides a technical solution: a device for processing and preserving umbilical cord mesenchymal stem cells, including a preservation box 1. The surface of the preservation box 1 has a plurality of placement holes 2 symmetrically arranged. The preservation box 1 holds a plurality of storage tubes 3 for preserving umbilical cord mesenchymal stem cells through the placement holes 2. A connecting cap 4 for sealing the inner wall of each placement hole 2 is threaded onto the inner sidewall. The surface of the preservation box 1 also has a first placement groove 5 and a second placement groove 6. A syringe 7 and an injection tube 8 are respectively placed inside the first placement groove 5 and the second placement groove 6. The injection tube 8 can be connected to the end of the syringe 7 and inserted into the storage tube 3 to replace the preservation solution inside the storage tube 3. A cooler 9 is fixedly connected to the bottom of the inner sidewall of the preservation box 1. A wide-angle camera 10 is also fixedly connected to the inner sidewall of the preservation box 1. A carrying assembly for carrying the preservation box 1 is provided on the top of the preservation box 1.

[0052] Specifically, the storage tube 3 includes a receiving tube 301, an upper seal 302, and a lower seal 303. The upper seal 302 is encapsulated on the top of the receiving tube 301. The upper seal 302 includes a peripheral portion 3021, an inner portion 3022, and a puncture portion 3023. The peripheral portion 3021, the inner portion 3022, and the puncture portion 3023 are integrally formed. An annular cavity adapted to the wall thickness of the receiving tube 301 is formed between the peripheral portion 3021 and the inner portion 3022 to allow the peripheral portion 3022 to... The inner wall of 1 and the outer wall of the inner connection 3022 are movably connected to the outer wall and inner wall of the receiving tube 301, respectively. The puncture part 3023 is fixed on the inner wall of the inner connection. The top center of the puncture part 3023 is provided with an insertion hole 11 for the injection tube 8 to pass through. A rubber stopper 12 is provided between the inner connection 3022 and the puncture part 3023. The puncture part 3023 punctures into the interior of the rubber stopper 12. The lower seal 303 is sealed at the bottom of the receiving tube 301.

[0053] In this embodiment, after both the lower seal 303 and the upper seal 302 are installed, a storage tube 3 for containing umbilical cord mesenchymal stem cells can be formed. In addition, a rubber stopper 12 is provided between the inner part 3022 and the puncture part 3023. The puncture part 3023 is inserted into the rubber stopper 12. There are two ways to preserve umbilical cord mesenchymal stem cells in the storage tube 3. The first method is to first place the umbilical cord mesenchymal stem cells into the already sealed receiving tube 301 and then seal it with the upper seal 302. The second method is to first install both the lower seal 303 and the upper seal 302 and then inject the umbilical cord mesenchymal stem cells with preservation fluid into the receiving tube 301 through the injection tube 8 through the puncture hole 11 at the top of the puncture part 3023.

[0054] Specifically, annular protrusions 13 are integrally formed on the outer wall of the receiving tube 301 at positions corresponding to the upper seal 302 and the lower seal 303. The two annular protrusions 13 are movably connected to the inner wall of the outer periphery 3021 and the inner wall of the lower seal 303, respectively.

[0055] In this embodiment, the annular protrusion 13 can ensure the stability of the installation of the upper seal 302 and the lower seal 303, and prevent the upper seal 302 and the lower seal from easily falling off.

[0056] Specifically, the lower seal 303 includes an outer part 3031 and an injection part 3032 with an opening. A magnetic ring 14 is fixedly connected to the inner wall of the outer part 3031, and the inner wall of the magnetic ring 14 is fixedly connected to the surface of the injection part 3032. A T-shaped rod 15 is fixedly connected to the top of the injection part 3032. A retaining spring 16 is sleeved on the surface of the T-shaped rod 15. One end of the retaining spring 16 is fixedly connected to the surface of the magnetic guide plate 17, and the magnetic guide plate 17 is movably sleeved on the surface of the T-shaped rod 15. An injection assembly for extracting preservation fluid is provided at the bottom of the storage box 1. It is assembled such that when the storage tube 3 is stored inside the storage box 1, the magnetic guide plate 17 is disengaged from the magnetic ring 14 so that the injection tube 8 extracts the old preservation fluid while the new preservation fluid is injected.

[0057] The injection assembly includes an injection tube 19, a control valve 20, and an injection pump 21. The control valve 20 is fixedly connected to the bottom of the storage box 1. The injection pump 21 is fixedly connected to the outlet of the control valve 20. The injection tube 19 is fixedly connected to the inlet of the control valve 20 and passes through the storage box 1 and is inserted into the interior of the lower seal 303.

[0058] In this embodiment, after the storage tube 3 is fixed inside the storage box 1, the injection tube 19 passes through the positioning member 22 and is inserted into the storage tube 3, so that the magnetic plate 17 is separated from the magnetic ring 14. Then, the injection tube 8 is inserted into the receiving tube 301. At the same time, the control valve 20 is opened and the injection pump 21 is started to inject new preservation fluid into the receiving tube 301. During the extraction process of the injection tube 8, the new preservation fluid is replaced with the old preservation fluid to achieve the replacement of the preservation fluid.

[0059] Specifically, a positioning element 22 is movably sleeved on the surface of the lower seal 303, and a guide rod 23 is connected to the surface of the positioning element 22. One end of the guide rod 23 movably passes through the positioning element 22 and is fixedly connected to the bottom of the inner wall of the storage box 1, while the other end of the guide rod 23 is fixedly connected to the top of the inner wall of the storage box 1. A return spring 24 is sleeved on the surface of the guide rod 23, with one end of the return spring 24 fixedly connected to the surface of the positioning element 22 and the other end of the return spring 24 fixedly connected to the top of the inner wall of the storage box 1. A first rubber ring 25 is fixedly sleeved on the surface of the positioning element 22, and a second rubber ring 26 is fixedly sleeved on the surface of the upper seal 302. The surface of the second rubber ring 26 is movably connected to the inner wall of the storage box 1. A positioning component for limiting the storage tube 3 is provided at the placement hole 2 on the storage box 1.

[0060] The positioning assembly includes a positioning block 27, a positioning spring 28, and a positioning plate 29. The surface of the positioning block 27 is fixedly connected to one end of the positioning spring 28, and the other end of the positioning spring 28 is fixedly connected to the inner wall of the placement hole 2. The positioning plate 29 is used to limit the positioning block 27.

[0061] In this embodiment, when the storage tube 3 is installed, its surface will press against the positioning block 27, causing it to move. When the top surface of the storage tube 3, that is, the surface of the upper seal 302, contacts the bottom of the positioning block 27, the positioning block 27 contacts the surface of the upper seal 302 under the action of the positioning spring 28, thereby locking and limiting its position.

[0062] Specifically, the carrying assembly includes a telescopic rod 30 and a carrying tube 31. The two telescopic rods 30 are movably connected to the top of the storage box 1. One end of the telescopic rod 30 is fixedly connected to a sealing cover 32, and the other end of the telescopic rod 30 is fixedly connected to a fixing block 33. A threaded ring 34 is movably sleeved on the surface of the fixing block 33. One end of the carrying tube 31 can be movably connected to the sealing cover 32, and the other end of the carrying tube 31 can be threadedly connected to the threaded ring 34. A threaded rod 35 is connected to the inside of the carrying tube 31 through a drive motor. A lifting member 36 is threadedly sleeved on the surface of the threaded rod 35. A limiting block 37 connected to the inner wall of the carrying tube 31 is provided on the surface of the lifting member 36. A surface fixing member 38 for installing the injection tube 8 is also connected to the surface of the lifting member 36.

[0063] The fixing member 38 includes an annular portion 3801 and a fastening portion 3802. The annular portion 3801 has an internal thread 39, and the injection tube 8 has an external thread 40. The fastening portion 3802 is fixedly connected to the surface of the annular portion 3801, and the surface of the fastening portion 3802 engages with the slot 41 on the lifting member 36. A spring piece 42 is fixedly connected to the surface of the fastening portion 3802, and one end of the spring piece 42 is fixedly connected to the surface of the annular portion 3801. A magnet block 43 is fixedly connected to the bottom of the annular portion 3801. The magnet block 43 can press the positioning block 27 to separate it from the top surface of the upper sealing member 302. The upper sealing member 302 is made of magnetic material, and the surface of the carrying tube 31 has a notch 18.

[0064] In this embodiment, the above technical solution not only enables the replacement of the preservation fluid, but also facilitates the removal of the storage tube 3 from the storage box 1. After the storage tube 3 is removed, the first rubber ring 25 on the positioning member 22 will contact the placement hole 2 to achieve a seal. The connection cover 4 is then used for sealing, which ensures that when one of the storage tubes 3 is removed, the internal cold air will not leak, thus preventing temperature imbalance.

[0065] A method for processing and preserving umbilical cord mesenchymal stem cells, using the aforementioned device for processing and preserving umbilical cord mesenchymal stem cells, such as... Figure 21This includes the following steps:

[0066] S1. Ensure that the storage tubes and other components inside the storage box are clean and sterile, and prepare the storage solution to ensure proper storage conditions;

[0067] S2. Collect umbilical cord mesenchymal stem cells. During the surgery or collection process, collect the umbilical cord mesenchymal stem cells into a suitable container or collector.

[0068] S3. Transfer the collected umbilical cord mesenchymal stem cells to the storage tube, open the connecting cover on the storage box, insert the storage tube into the placement hole of the storage box, ensure that the connecting cover is tightly sealed, and the bottom of the storage box is fixedly connected to a cooler to ensure that the inside of the storage box maintains a suitable temperature to prolong the survival period of the stem cells.

[0069] S4. During the preservation process, the preservation solution needs to be changed regularly to maintain the excellent condition of the stem cells. Open the first placement slot on the preservation box, take out the syringe, connect the injection tube, insert the injection tube into the storage tube, draw out the old preservation solution from the storage tube, and inject the preservation solution using the injection component at the bottom of the preservation box.

[0070] S5. Using a wide-angle camera fixedly connected to the preservation box, the status of stem cells in the preservation box can be monitored, and relevant information of stem cells in the preservation box, such as preservation time and preservation solution replacement records, can be recorded regularly.

[0071] S6. When stem cells are needed, remove them from the storage tube for further processing or application.

[0072] During use, place the umbilical cord mesenchymal stem cells that need to be preserved into the storage tube 3, such as... Figure 5-7 As shown, the storage tube 3 includes a receiving tube 301, an upper seal 302, and a lower seal 303. The upper seal 302 is sealed to the top of the receiving tube 301, and the lower seal 303 is sealed to the bottom of the receiving tube 301. Additionally, as shown... Figure 11 As shown, the upper seal 302 includes an outer portion 3021, an inner portion 3022, and a piercing portion 3023. The inner portion 3022 and the piercing portion 3023 are integrally formed. The piercing portion 3023 is fixed on the inner sidewall of the inner portion. An insertion hole 11 is provided at the top of the piercing portion 3023, and the insertion hole 11 is located at the center of the piercing portion 3023. During installation, the annular cavity formed between the outer portion 3021 and the inner portion 3022 is aligned with the top of the receiving tube 301. The wall thickness of the receiving tube 301 is adapted to the annular cavity, so that the upper seal 302 can be snapped onto the top of the receiving tube 301. Furthermore, annular protrusions 13 are integrally formed on the outer sidewall of the receiving tube 301 at positions corresponding to the upper seal 302. The annular protrusions 13 are movably engaged with the inner sidewall of the outer portion 3021, which can enhance the stability of the connection.

[0073] After both the lower seal 303 and the upper seal 302 are installed, a storage tube 3 for containing umbilical cord mesenchymal stem cells can be formed. In addition, a rubber stopper 12 is provided between the inner part 3022 and the puncture part 3023. The puncture part 3023 is inserted into the rubber stopper 12. There are two ways to preserve umbilical cord mesenchymal stem cells in the storage tube 3. The first method is to first place the umbilical cord mesenchymal stem cells into the already sealed receiving tube 301 and then seal it with the upper seal 302. The second method is to first install both the lower seal 303 and the upper seal 302 and then inject the umbilical cord mesenchymal stem cells containing preservation fluid into the receiving tube 301 through the injection tube 8 through the puncture hole 11 at the top of the puncture part 3023.

[0074] After saving, transfer storage tube 3 through the following method: Figure 3 The placement hole 2 shown is placed inside the storage box 1. A guide rod 23 is fixedly connected inside the storage box 1 at the position corresponding to the placement hole 2. The two ends of the guide rod 23 are fixedly connected to the top and bottom of the inner wall of the storage box 1, respectively. A positioning element 22 is movably sleeved on the guide rod 23. One end of the return spring 24 is fixedly connected to the surface of the positioning element 22, and the other end of the return spring 24 is fixedly connected to the top of the inner wall of the storage box 1. The bottom of the storage tube 3 is aligned with the position of the positioning element 22. The surface of the lower seal 303 will contact the inner wall of the positioning element 22. The storage tube 3 is pressed down to squeeze the positioning element 22, thereby causing it to move downward along the guide rod 23. The return spring 24 is stretched. When the surface of the positioning element 22 contacts the bottom of the inner wall of the storage box 1, the upper seal 302 corresponds to the position of the placement hole 2. A second rubber ring 26 is fixedly connected to the surface of the upper seal 302. The second rubber ring 26 will contact the inner wall of the placement hole 2, thereby achieving sealing and limiting. In order to make the storage tube 3 fixedly fixed, as Figure 3 and Figure 20 As shown, a positioning assembly is provided at the placement hole 2. The positioning assembly includes a positioning block 27, a positioning spring 28, and a positioning plate 29. One end of the positioning spring 28 is fixedly connected to the surface of the positioning block 27, and the other end is fixedly connected to the inner wall of the placement hole 2. The positioning plate 29 is fixedly connected inside the placement hole 2 and can limit the positioning block 27. The bottom of the positioning plate 29 is provided with a protrusion, and the positioning block 27 is provided with a groove. The protrusion can be inserted into the groove. When the storage tube 3 is installed, its surface will squeeze the positioning block 27, causing it to move. When the top surface of the storage tube 3, that is, the surface of the upper sealing member 302, contacts the bottom of the positioning block 27, the positioning block 27 contacts the surface of the upper sealing member 302 under the action of the positioning spring 28, thereby locking and limiting its position.

[0075] After placing all the storage tubes 3 into the storage box 1, immediately thread the connecting cap 4 onto the placement hole 2 to further seal the storage tubes 3 and prevent dust contamination. Then, power on the internal cooler 9 to maintain the internal temperature of the storage box 1 at 4-6℃, reducing the activity of umbilical cord mesenchymal stem cells. The preservation solution can be the one disclosed in publication number CN113973806B, or other existing preservation solutions can be used to preserve umbilical cord mesenchymal stem cells for a short period of time so that their activity can be restored in a timely manner during subsequent use.

[0076] Because umbilical cord mesenchymal stem cells are in a low-activity state, the nutrients in the preservation solution will be absorbed after a certain period of time, and the waste products produced by cell metabolism will also be in the preservation solution. In order to preserve umbilical cord mesenchymal stem cells healthily and stably in the container 301, the preservation solution inside needs to be replaced after a certain period of time.

[0077] like Figure 1 and Figure 3-4 As shown, a first placement groove 5 and a second placement groove 6 are also provided on the surface of the preservation box 1. A syringe 7 is placed inside the first placement groove 5, and an injection tube 8 is placed inside the second placement groove 6. The injection tube 8 can be connected to the end of the syringe 7, so that the injection tube 8 can be inserted into the storage tube 3, that is, inserted through the puncture hole 11 at the top center of the puncture part 3023, passing through the rubber stopper 12 and entering the interior of the receiving tube 301. The preservation fluid can be extracted by the syringe 7, and then new preservation fluid can be injected into the syringe 7. The wide-angle camera 10 fixed inside the preservation box 1 can penetrate into the interior of the transparent receiving tube 301. Of course, the wide-angle camera 10 can be equipped with a supplementary light. The preservation fluid can be injected into the interior of the receiving tube 301 through the injection tube 8 to replace the preservation fluid, so that the umbilical cord mesenchymal stem cells are kept in a stable environment.

[0078] The above treatment allows for the replacement of the preservation solution, enabling umbilical cord mesenchymal stem cells to remain in a low-activity state for an extended period, which is beneficial for subsequent use.

[0079] However, the above method requires that when extracting the waste preservation solution, all the old preservation solution must be extracted before injecting the new preservation solution. Due to the presence of umbilical cord mesenchymal stem cells, the extraction may not be complete, which may affect the activity of umbilical cord mesenchymal stem cells after the new preservation solution is injected.

[0080] like Figure 10As shown, the lower seal 303 includes an outer part 3031 and an injection part 3032 with an opening. A magnetic ring 14 is fixedly connected to the inner wall of the outer part 3031. The inner wall of the magnetic ring 14 is fixedly connected to the surface of the injection part 3032. A T-shaped rod 15 is fixedly connected to the top of the injection part 3032. A magnetic guide plate 17 is movably sleeved on the surface of the T-shaped rod 15. A retaining spring 16 is fixed on the magnetic guide plate 17 and is fixedly connected to the T-shaped rod 15. Before the storage tube 3 is stored inside the storage box 1, the magnetic guide plate 17 is in contact with and fixed to the magnetic ring 14 under the action of the retaining spring 16, which can ensure that the internal storage liquid will not leak out. After the storage tube 3 is placed inside the storage box 1, the injection assembly at the bottom of the storage box 1 will disengage the magnetic guide plate 17 from the magnetic ring 14 so that the injection tube 8 draws out the old storage liquid while the new storage liquid is injected. Figure 2 and Figure 5-7 As shown, the injection assembly includes an injection tube 19, a control valve 20, and an injection pump 21. The control valve 20 is fixedly connected to the bottom of the storage box 1, the injection pump 21 is fixedly connected to the outlet of the control valve 20, and the injection tube 19 is fixedly connected to the inlet of the control valve 20. After the storage tube 3 is fixed inside the storage box 1, the injection tube 19 passes through the positioning member 22 and is inserted into the storage tube 3, causing the magnetic plate 17 to separate from the magnetic ring 14. Then, the injection tube 8 is inserted into the receiving tube 301. At the same time, the control valve 20 is opened, the injection pump 21 is started, and new storage solution is injected into the receiving tube 301. During the extraction process of the injection tube 8, the new storage solution is replaced with the old storage solution to achieve the replacement of the storage solution.

[0081] The above technical solution enables the replacement of the preservation fluid. In addition, the top of the preservation box 1 is equipped with a carrying component for carrying the preservation box 1, which can facilitate short-distance movement of the preservation box 1. However, by changing the power supply of the cooler 9 to battery power, a longer-distance transport can be achieved to deal with emergencies.

[0082] The carrying assembly includes a telescopic rod 30 and a carrying tube 31. The telescopic rod 30 is movably connected to the top of the storage box 1 and can rotate. One end of the telescopic rod 30 is fixedly connected to a sealing cover 32, and a threaded ring 34 is movably fitted onto the surface of the other fixing block 33. When installing the carrying tube 31, one end is movably connected to the sealing cover 32, and the other end corresponds to the fixing block 33. Rotating the threaded ring 34 connects it to the carrying tube 31, which enables the transfer of the storage box 1.

[0083] Additionally, the inside of the carrying tube 31 is connected to a threaded rod 35 via a drive motor. A lifting member 36 is threadedly fitted onto the surface of the threaded rod 35, and a limit block 37 is fixedly connected to the lifting member 36, contacting the inner wall of the carrying tube 31. The lifting member 36 is annular. One of the connecting caps 4 is removed, allowing the surface of the carrying tube 31 to align with the placement hole 2. The carrying tube 31 has a drive motor power interface; connecting the drive motor allows the threaded rod 35 to rotate, thus enabling the lifting member 36 to move up and down. The surface of the lifting member 36 is also connected to a surface fixing member 38 for mounting the injection tube 8. The surface of the injection tube 8 has an external thread 40, and the inside of the annular portion 3801 has an internal thread 39. During installation, the injection tube 8 is threaded onto the annular portion 3801, allowing the injection tube 8 to move and be easily inserted directly into the receiving tube 301. The fixing member 38 includes an annular portion 3801 and a fastening portion 3802. Figure 18 As shown, the fastening part 3802 is fixedly connected to the surface of the annular part 3801 and engages with the slot 41 on the lifting member 36. At the same time, a magnet 43 is fixedly connected to the bottom of the annular part 3801. When the storage liquid inside the storage tube 301 is not replaced, the positioning block 27 can be squeezed by the magnet 43, causing it to move and detach from the top surface of the upper seal 302. The upper seal 302 is made of magnetic material. At this time, the magnet 43 attracts the upper seal 302. Then the lifting member 36 moves upward, and the storage tube 3 can be pulled out, so that the hand does not directly contact it, making it easy to pull out. The surface of the carrying tube 31 has a notch 18. After rising, the fastening part 3802 corresponds to the notch 18. Squeezing the fastening part 3802 deforms the spring piece 42, thereby detaching it from the slot 41. The inner diameter of the lifting member 36 is larger than the diameter of the storage tube 3, so the storage tube 3 can be taken out.

[0084] The above technical solution allows for the replacement of the storage fluid without disassembling the storage tube 3. It also facilitates the removal of the storage tube 3 from the storage box 1. After the storage tube 3 is removed, the first rubber ring 25 on the positioning component 22 will contact the placement hole 2 to achieve a seal. The connection cover 4 is then used for sealing. This ensures that when one of the storage tubes 3 is removed, the internal cold air will not leak, thus preventing temperature imbalance.

[0085] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0086] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A device for processing and preserving umbilical cord mesenchymal stem cells, comprising a preservation box (1), characterized in that: The surface of the preservation box (1) is symmetrically provided with a number of placement holes (2). The preservation box (1) is provided with a number of storage tubes (3) for preserving umbilical cord mesenchymal stem cells through the placement holes (2). The inner sidewall of the placement hole (2) is threaded with a connecting cap (4) for sealing it. The storage tube (3) includes a receiving tube (301), an upper seal (302) and a lower seal (303). The upper seal (302) is encapsulated on the top of the receiving tube (301). The upper seal (302) includes a peripheral portion (3021), an inner portion (3022), and a puncture portion (3023). The peripheral portion (3021), the inner portion (3022), and the puncture portion (3023) are integrally formed. An annular cavity adapted to the wall thickness of the receiving tube (301) is formed between the peripheral portion (3021) and the inner portion (3022) to allow the inner side of the peripheral portion (3021) to be exposed. The outer walls of the inner wall and the inner connection (3022) are movably connected to the outer wall and the inner wall of the receiving tube (301), respectively. The puncture part (3023) is fixed on the inner wall of the inner connection. The top center of the puncture part (3023) is provided with an insertion hole (11) for the injection tube (8) to pass through. A rubber plug (12) is provided between the inner connection (3022) and the puncture part (3023). The puncture part (3023) pierces into the interior of the rubber plug (12). The lower seal (303) is encapsulated at the bottom of the receiving tube (301); On the outer wall of the accommodating tube (301), at the positions corresponding to the upper seal (302) and the lower seal (303), an annular protrusion (13) is integrally formed. The two annular protrusions (13) are movably connected to the inner wall of the outer periphery (3021) and the inner wall of the lower seal (303), respectively. The lower seal (303) includes an outer part (3031) and an injection part (3032) with an opening. A magnet ring (14) is fixedly connected to the inner wall of the outer part (3031), and the inner wall of the magnet ring (14) is fixedly connected to the surface of the injection part (3032). A T-shaped rod (15) is fixedly connected to the top of the injection part (3032). A retaining spring (16) is sleeved on the surface of the T-shaped rod (15). One end of the retaining spring (16) is fixedly connected to the surface of the magnetic plate (17). The magnetic plate (17) is movably sleeved on the surface of the T-shaped rod (15). The bottom of the storage box (1) is provided with an injection assembly for extracting the storage liquid, which is configured such that when the storage tube (3) is stored inside the storage box (1), the magnetic plate (17) is separated from the magnetic ring (14) so ​​that the injection tube (8) extracts the old storage liquid while the new storage liquid is injected. The surface of the storage box (1) is also provided with a first placement groove (5) and a second placement groove (6). The first placement groove (5) and the second placement groove (6) are respectively placed with a syringe (7) and an injection tube (8). The injection tube (8) can be connected to the end of the syringe (7) and inserted into the storage tube (3) to replace the storage liquid inside the storage tube (3). A cooler (9) is fixedly connected to the bottom of the inner wall of the storage box (1), and a wide-angle camera (10) is also fixedly connected to the inner wall of the storage box (1). A carrying component for carrying the storage box (1) is provided on the top of the storage box (1). The carrying assembly includes a telescopic rod (30) and a carrying tube (31). The two telescopic rods (30) are movably connected to the top of the storage box (1). One of the telescopic rods (30) is fixedly connected to a closing cover (32) at its end, and the other telescopic rod (30) is fixedly connected to a fixing block (33) at its end. A threaded ring (34) is movably sleeved on the surface of the fixing block (33). One end of the carrying tube (31) can be movably connected to the closing cap (32), and the other end of the carrying tube (31) can be threadedly connected to the threaded ring (34). The inside of the carrying tube (31) is connected to a threaded rod (35) via a drive motor. A lifting member (36) is threadedly sleeved on the surface of the threaded rod (35). A limiting block (37) connected to the inner wall of the carrying tube (31) is provided on the surface of the lifting member (36). A surface fixing member (38) for installing the injection tube (8) is also connected to the surface of the lifting member (36). The fastener (38) includes an annular portion (3801) and a fastening portion (3802). The annular portion (3801) has an internal thread (39) inside, and the injection tube (8) has an external thread (40) on its surface. The fastening portion (3802) is fixedly connected to the surface of the annular portion (3801), and the surface of the fastening portion (3802) engages with a slot (41) on the lifting member (36). A spring piece (42) is fixedly connected to the surface of the fastening part (3802). One end of the spring piece (42) is fixedly connected to the surface of the annular part (3801). A magnet block (43) is fixedly connected to the bottom of the annular part (3801). The magnet block (43) can press the positioning block (27) to separate it from the top surface of the upper seal (302). The upper seal (302) is made of magnetic material. A notch (18) is opened on the surface of the carrying tube (31).

2. The device for processing and preserving umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The injection assembly includes an injection tube (19), a control valve (20), and an injection pump (21). The control valve (20) is fixedly connected to the bottom of the storage box (1). The injection pump (21) is fixedly connected to the outlet of the control valve (20). The injection tube (19) is fixedly connected to the inlet of the control valve (20) and passes through the storage box (1) and is inserted into the interior of the lower seal (303).

3. The device for processing and preserving umbilical cord mesenchymal stem cells according to claim 2, characterized in that: The lower seal (303) is movably sleeved with a positioning member (22), and a guide rod (23) is connected to the surface of the positioning member (22). One end of the guide rod (23) movably passes through the positioning member (22) and is fixedly connected to the bottom of the inner wall of the storage box (1). The other end of the guide rod (23) is fixedly connected to the top of the inner wall of the storage box (1). A reset spring (24) is sleeved on the surface of the guide rod (23). One end of the reset spring (24) is fixedly connected to the surface of the positioning member (22), and the other end of the reset spring (24) is fixedly connected to the top of the inner wall of the storage box (1). The surface of the positioning member (22) is fixedly sleeved with a first rubber ring (25), and the surface of the upper sealing member (302) is fixedly sleeved with a second rubber ring (26). The surface of the second rubber ring (26) is movably connected to the inner wall of the storage box (1). The storage box (1) is provided with a positioning component at the placement hole (2) for limiting the position of the storage tube (3).

4. The device for processing and preserving umbilical cord mesenchymal stem cells according to claim 3, characterized in that: The positioning component includes a positioning block (27), a positioning spring (28), and a positioning plate (29). The surface of the positioning block (27) is fixedly connected to one end of the positioning spring (28), and the other end of the positioning spring (28) is fixedly connected to the inner wall of the placement hole (2). The positioning plate (29) is used to limit the positioning block (27).

5. A method for processing and preserving umbilical cord mesenchymal stem cells, characterized in that, A processing and preservation apparatus for umbilical cord mesenchymal stem cells according to any one of claims 1-4, comprising the following steps: S1. Ensure that the storage tubes and other components inside the storage box are clean and sterile, and prepare the storage solution to ensure proper storage conditions; S2. Collect umbilical cord mesenchymal stem cells. During the surgery or collection process, collect the umbilical cord mesenchymal stem cells into a suitable container or collector. S3. Transfer the collected umbilical cord mesenchymal stem cells to the storage tube, open the connecting cover on the storage box, insert the storage tube into the placement hole of the storage box, ensure that the connecting cover is tightly sealed, and the bottom of the storage box is fixedly connected to a cooler to ensure that the inside of the storage box maintains a suitable temperature to prolong the survival period of the stem cells. S4. During the preservation process, the preservation solution needs to be changed regularly to maintain the excellent condition of the stem cells. Open the first placement slot on the preservation box, take out the syringe, connect the injection tube, insert the injection tube into the storage tube, draw out the old preservation solution from the storage tube, and inject the preservation solution using the injection component at the bottom of the preservation box. S5. Using a wide-angle camera fixedly connected to the preservation box, the status of stem cells in the preservation box can be monitored, and relevant information of stem cells in the preservation box, such as preservation time and preservation solution replacement records, can be recorded regularly. S6. When stem cells are needed, remove them from the storage tube for further processing or application.

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