A fully automatic sputum staining and making system and method

Abstract

The application discloses a kind of full-automatic sputum dyeing system and method of slice, the system of the present application will three-dimensional mechanical transmission device, sputum homogenization device, consumable storage device, slice mechanical device, glass slide collection device, oil drop device, dyeing reagent input device and controller are integrated in rack, using PLC programmable controller to automatically control the system, automatically complete to sputum homogenization, dyeing and slice, complete the whole process of sputum smear production.The present application has the advantages of high degree of automation, low cost, high sputum smear production efficiency and good sputum smear production quality, meets the needs of medical system for sputum smear production, reduces the probability of infection to medical staff.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medical devices and medical examination technology, and particularly relates to a full-automatic sputum staining and sheet making system and method. BACKGROUND

[0002] Sputum is a secretion produced by alveoli, bronchi and trachea. Sputum examination can find out pneumonia, tuberculosis and fungal infection of the lung, and some diseases of the respiratory system, and can observe the curative effect and prognosis during the treatment. Sputum examination includes sputum microscopic examination, sputum cytological examination, sputum smear examination and the like. Sputum culture

[0003] Sputum smear examination as a simple and effective sputum processing method can help doctors to preliminarily diagnose the corresponding diseases quickly. In the traditional sputum smear making process, the processes of homogenization, staining and sheet making need to be completed manually. However, in the manual completion process, the following problems exist: sputum may cause infection to medical staff due to the large amount of viruses or bacteria hidden in the sputum itself; the time spent in the processing of sputum samples is relatively long, and the steps for completing a qualified sputum smear are relatively complicated; the staining process of each sputum sample may have a large random error, resulting in uneven quality of sputum smears; the instruments used in the sputum processing process need to be frequently cleaned or replaced; there are many patients who need sputum smear examination, and the examination efficiency is too low. The existing technology has the problems of high examination cost, low sputum smear sheet making efficiency and uneven quality of sputum smears. SUMMARY

[0004] The present application aims at the deficiencies of the prior art and provides a full-automatic sputum staining and sheet making system and method. The system of the present application integrates a three-dimensional mechanical transmission device, a sputum homogenization device, a consumable storage device, a sheet making mechanical device, a glass slide collection device, an oil dropping device, a staining reagent input device and a controller in a rack, and uses a PLC programmable controller to automatically control the system, to automatically complete the homogenization, staining and sheet making of sputum, and to complete the whole process of sputum smear making. The present application has the advantages of high automation, low cost, high sputum smear making efficiency and good sputum smear making quality, and meets the needs of the medical system for sputum smear making and reduces the infection probability of medical staff.

[0005] The specific technical scheme for achieving the object of the present application is as follows:

[0006] A full-automatic sputum staining and sheet making system, characterized in that the system comprises a rack, a three-dimensional mechanical transmission device, a sputum homogenization device, a consumable storage device, a sheet making mechanical device, a glass slide collection device, an oil dropping device, a staining reagent input device and a controller.

[0007] ​The panel of the rack is provided with a computer display screen, the panel is provided with an ultraviolet lamp, and the bottom of the rack is provided with a garbage can;

[0008] The three-dimensional mechanical transmission device is composed of an X-direction moving module, a Y-direction moving module and a Z-direction moving module,

[0009] The X-direction moving module comprises an X-direction slider, an X-direction stepping motor and an X-direction slide;

[0010] The X-direction slide is provided with an X-direction photoelectric sensing switch, and the X-direction slider is provided with an X-direction inductor baffle;

[0011] The Y-direction moving module comprises a Y-direction slider, a Y-direction stepping motor and a Y-direction slide;

[0012] The Y-direction stepping motor drives the Y-direction slider to run on the Y-direction slide;

[0013] The Y-direction slide is provided with a Y-direction photoelectric sensing switch, and the Y-direction slider is provided with a Y-direction inductor baffle and a suction pump;

[0014] The suction pump is provided with a suction piston and a photoelectric sensing switch of the suction pump, and the suction piston is provided with an inductor baffle of the suction pump;

[0015] The Z-direction moving module comprises a Z-direction connecting platform, a Z-direction first slide, a Z-direction second slide, an electric claw stepping motor, an electric claw motor slider, a pipetting stepping motor and a pipetting motor slider;

[0016] The Z-direction first slide and the Z-direction second slide are arranged below the Z-direction connecting platform;

[0017] The electric claw stepping motor and the pipetting stepping motor are arranged on the Z-direction connecting platform;

[0018] The electric claw stepping motor drives the electric claw motor slider to run on the Z-direction first slide;

[0019] The pipetting stepping motor drives the pipetting motor slider to run on the Z-direction second slide;

[0020] The three-dimensional mechanical transmission device is arranged on the top of the rack 1 through the X-direction slide of the X-direction moving module, the Y-direction slide of the Y-direction moving module is arranged on the X-direction slider, and the Z-direction connecting platform of the Z-direction moving module is arranged on the Y-direction slider;

[0021] The sputum homogenizing device is composed of a driving base, a shaking platform, a shaking stepping motor, a crankshaft and a shaking motor belt driving wheel;

[0022] The oscillation step motor is arranged on the driving base, the crankshaft is four pieces, and is hinged to the driving base. The oscillation step motor drives the four crankshafts to rotate synchronously through the oscillation motor belt drive wheel. The oscillation platform is hinged to the crankshaft.

[0023] The oscillation motor belt drive wheel is provided with an inductor blocking piece of the oscillation motor. The inner side of the driving base is provided with a photoelectric induction switch of the oscillation motor.

[0024] A plurality of sputum cups are arranged on the oscillation platform.

[0025] The sputum homogenization device is arranged at the bottom of the rack 1 through the driving base.

[0026] The consumable storage device is composed of a consumable platform frame, a container storage disc, a heater, a liquid pumping electromagnetic valve and a liquid pumping pump.

[0027] The liquid pumping electromagnetic valve is a plurality of pieces, which are distributed around the consumable platform frame. The heater is arranged on one side of the consumable platform frame. The liquid pumping pump is arranged beside the consumable platform frame. The container storage disc is arranged above the consumable platform frame. A plurality of centrifugal filter type sandwich cups, a plurality of liquefied sputum pipettors and a plurality of digestive liquid pipettors are sequentially arranged on the container storage disc.

[0028] The consumable storage device is arranged at the bottom of the rack through the consumable platform frame.

[0029] The slide making mechanical device is composed of a slide making machine support, a slide making operation platform and a Z direction operation platform step motor.

[0030] The Z direction slide making slide block is driven by the Z direction operation platform step motor to run on the Z direction slide making slide. The slide making operation platform is arranged on the Z direction slide making slide block.

[0031] The operation platform photoelectric induction switch is arranged at the bottom end of the slide making machine support. The operation platform inductor blocking piece is arranged on the Z direction slide making slide block.

[0032] The suction disc connecting platform and the suction disc step motor are arranged on the slide making operation platform.

[0033] The Z direction suction disc slide and the Z direction suction disc slide block are arranged on the slide making operation platform. The suction disc step motor drives the Z direction suction disc slide block to run on the Z direction suction disc slide. The suction disc connecting platform is arranged on the Z direction suction disc slide block.

[0034] The cover glass suction valve and the cover glass pump are arranged on the suction disc connecting platform.

[0035] The suction disc platform photoelectric induction switch is arranged on the Z direction suction disc slide of the slide making operation platform. The suction disc platform inductor blocking piece is arranged on the suction disc connecting platform.

[0036] The suction cup connection platform is equipped with a suction cup rotating stepper motor and a suction cup rotating arm. The suction cup rotating stepper motor drives the suction cup rotating arm to swing on the suction cup connection platform.

[0037] The suction cup rotating stepper motor is provided with a suction cup rotating arm photoelectric sensor switch on the rear side, and a suction cup rotating arm sensor baffle is provided at one end of the suction cup rotating arm.

[0038] The suction cup rotating arm is equipped with a vacuum suction cup; the suction cup connecting platform is equipped with a cover glass pump connected to the vacuum suction cup via a cover glass suction valve;

[0039] The slide operation platform is equipped with a Y-direction slide stepper motor and a slide moving bracket. A slide is placed on the slide moving bracket. The Y-direction slide stepper motor touches the slide via the slide moving bracket to move it along the Y direction.

[0040] The slide moving support is provided with a slide Y-axis sensor baffle, and one end of the Y-axis slide stepper motor is provided with a slide Y-axis photoelectric sensor switch.

[0041] The slide operation platform is equipped with a Z-direction slide stepper motor and a slide storage box; the slide storage box is equipped with a slide lifting rod, and a slide is placed inside the slide storage box; the Z-direction slide stepper motor touches the slide in the slide storage box via the slide lifting rod to move the slide along the Z direction.

[0042] One end of the slide lifting rod is equipped with a storage box sensor baffle, and the slide operation platform is equipped with a storage box photoelectric sensor switch.

[0043] The slide operation platform is also equipped with an oil suction solenoid valve, a cover slide storage box, and an oil dripping needle.

[0044] The cover glass storage box contains a cover glass;

[0045] The film-making machinery is mounted at the bottom of the frame via a film-making machine support;

[0046] The slide collecting device consists of an X-direction collecting and moving module and a slide collecting structure.

[0047] The X-direction collecting and moving module includes: an X-direction collecting slider, an X-direction collecting stepper motor, a collecting motor belt drive wheel, and an X-direction collecting slide; the X-direction collecting stepper motor drives the X-direction collecting slider to run on the X-direction collecting slide via the collecting motor belt drive wheel;

[0048] The glass slide collection frame is mounted on the X-direction collection slider;

[0049] The left and right ends of the X-direction collection slide are provided with collection photoelectric induction switches, and the X-direction collection slider is provided with a collection sensor blocking piece;

[0050] The slide collection device is arranged at the bottom of the rack through the X-direction collection slide;

[0051] The oil dripping device is composed of an oil dripping support, an oil injection piston, an oil dripping stepping motor, an oil injection electromagnetic valve, an oil dripping motor belt driving wheel, an oil injection transmission shaft and a contact sensor switch of the oil dripping motor;

[0052] The oil dripping stepping motor is connected with the oil injection transmission shaft through the oil dripping motor belt driving wheel, and the oil injection transmission shaft is connected with the oil injection piston;

[0053] The contact sensor switch of the oil dripping motor is composed of two parts, which are arranged on the upper and lower sides of the oil dripping support respectively, the oil dripping motor sensor blocking piece is arranged on the oil injection transmission shaft, and the oil dripping motor sensor blocking piece moves between the contact sensor switches of the oil dripping motor on the upper and lower sides;

[0054] The oil injection electromagnetic valve and the oil sucking electromagnetic valve of the tablet making device are electrically connected with the oil dripping stepping motor;

[0055] The oil injection piston is provided with an oil inlet nozzle and an oil outlet nozzle, the oil inlet nozzle is connected with the oil sucking electromagnetic valve of the tablet making device and an oil injection bottle, and the oil outlet nozzle is connected with the oil injection electromagnetic valve and a dripping needle head of the tablet making device;

[0056] The oil dripping device is arranged at the bottom of the rack through the oil dripping support;

[0057] The staining reagent input device is composed of a staining pump support, a staining reagent bottle rack and a two-dimensional mechanical transmission device;

[0058] The staining pump support is provided with a plurality of staining pumps inside the support and a plurality of staining electromagnetic valves outside the support;

[0059] The staining reagent bottle rack is provided with a plurality of staining reagent bottles and digestion liquid reagent bottles, and the plurality of staining reagent bottles are connected with the plurality of staining pumps and the plurality of staining electromagnetic valves one by one;

[0060] The staining pump support is further provided with a contact sensor switch in the X-direction of staining and a pipette buckle;

[0061] The two-dimensional mechanical transmission device is composed of an X-direction staining movement module and a Y-direction staining movement module;

[0062] The X-direction staining movement module comprises an X-direction staining slider, an X-direction staining stepping motor and an X-direction staining slide, and the X-direction staining stepping motor drives the X-direction staining slider to run on the X-direction staining slide;

[0063] The Y-direction dyeing moving module comprises a Y-direction dyeing slider, a Y-direction dyeing stepping motor and a Y-direction dyeing slide; the Y-direction dyeing stepping motor drives the Y-direction dyeing slider to run on the Y-direction dyeing slide;

[0064] The Y-direction dyeing slide of the Y-direction dyeing moving module is arranged on the X-direction dyeing slider;

[0065] The Y-direction dyeing slider is provided with a dropper;

[0066] The X-direction dyeing slider is provided with a sensor blocking piece in the X-direction;

[0067] The Y-direction dyeing slide is provided with a touch sensor switch in the Y-direction;

[0068] The dyeing reagent input device is sequentially arranged at the bottom of the rack through the dyeing pump support, the dyeing reagent bottle rack and the X-direction dyeing slide of the two-dimensional mechanical transmission device;

[0069] The controller is a PLC programmable controller, and the controller is electrically connected with the computer display screen of the rack and the electrical parts of the three-dimensional mechanical transmission device, the sputum homogenizing device, the consumable storage device, the tablet making mechanical device, the glass slide collecting device, the oil dropping device and the dyeing reagent input device.

[0070] The Z-direction moving module is provided with an electric claw motor slider, an electric claw on the electric claw motor slider, and a semicircular arc clamping nozzle on the electric claw;

[0071] The Z-direction moving module is provided with a digestive liquid injection pipeline on the pipette motor slider; a side pipeline on the digestive liquid injection pipeline is connected with a digestive liquid pump;

[0072] The Z-direction first slide top of the Z-direction moving module is provided with a photoelectric sensor switch of the electric claw motor and a sensor blocking piece of the electric claw motor on the electric claw motor slider; the Z-direction second slide top is provided with a photoelectric sensor switch of the pipette motor and a sensor blocking piece of the pipette motor on the pipette motor slider.

[0073] The Z-direction connecting platform of the Z-direction moving module is provided with a digestive liquid electromagnetic valve, a suction electromagnetic valve and a contour camera driving module; the digestive liquid electromagnetic valve is electrically connected with the digestive liquid pump, and the suction pump is electrically connected with the suction electromagnetic valve.

[0074] The plurality of centrifugal filter type interlayer cups are respectively connected with the plurality of suction electromagnetic valves through liquid pipes, and the suction pump is electrically connected with the suction electromagnetic valve.

[0075] A full-automatic sputum dyeing and tablet making method, characterized in that the method adopts the full-automatic sputum dyeing and tablet making system, and the specific implementation steps are as follows:

[0076] a. Consumable taking and placing step: before the system starts to work, the remaining vessels in the garbage can are emptied, 1-20 sputum cups are placed in the sputum homogenization device, 1-20 liquefied sputum pipettes, 1-20 centrifugal filter sandwich cups and 1-20 digestive solution pipettes are placed in the consumable storage device, 1-50 glass slides and 1-50 coverslips are placed in the slide making mechanical device, the microscope oil is added in the oil injection bottle, 3-5 staining reagent bottles and the digestive solution reagent bottle on the staining reagent bottle rack are respectively injected with 3-5 kinds of staining solution and digestive solution;

[0077] b. Resetting step: after the step a is performed, the system is started, and the controller is operated to reset the device.

[0078] c. Digestive solution injection step: after the step b is performed, the digestive solution injection pipeline of the Z direction moving module of the three-dimensional mechanical transmission device holds and lifts the digestive solution pipette on the consumable storage device, the digestive solution pump and the digestive solution electromagnetic valve are opened, the digestive solution in the digestive solution reagent bottle on the staining reagent input device is injected into the sputum cup on the sputum homogenization device through the digestive solution pipette, and then the digestive solution pipette is thrown into the garbage can. The injection is repeated for each sputum cup in step a;

[0079] d. Sample homogenization step: after the step c is performed, the oscillation stepping motor on the sputum homogenization device drives the sputum cup on the oscillation platform to oscillate clockwise or counterclockwise, the oscillation time is 5-10 minutes, and the homogenized sputum is obtained;

[0080] e. Sampling step: after the step d is performed, the digestive solution injection pipeline of the Z direction moving module of the three-dimensional mechanical transmission device holds and lifts the liquefied sputum pipette on the consumable storage device, the suction pump and the suction electromagnetic valve are opened, the homogenized sputum in the sputum cup on the sputum homogenization device is sucked into the liquefied sputum pipette, the digestive solution injection pipeline transfers the homogenized sputum to the centrifugal filter sandwich cup on the consumable storage device at this time, and then the liquefied sputum pipette is thrown into the garbage can. The sampling of the homogenized sputum in each sputum cup in step d is repeated;

[0081] f. Suction step: after the step e is performed, the suction electromagnetic valve and the suction pump on the consumable storage device are opened, and the excess homogenized sputum in the centrifugal filter sandwich cup is sucked out, the suction time is 1-3 minutes, the bacteria in the homogenized sputum adhere to the bottom of the centrifugal filter sandwich cup, and the sputum film is obtained;

[0082] g. Staining step: After performing the step f, the dropper of the two-dimensional mechanical transmission device on the staining reagent input device is moved above the centrifugal filter sandwich cup on the consumable storage device, the staining pump and the staining electromagnetic valve are opened, the staining liquid in one staining reagent bottle is dropped into the sputum film in the centrifugal filter sandwich cup, and is left for 1-5 minutes to complete the staining, and the stained film is obtained from the bottom of the centrifugal filter sandwich cup;

[0083] h. Repeating the steps f and g, the staining liquid in 3-5 staining reagent bottles is used to stain the stained film in turn to obtain multiple stained films;

[0084] i. Staining image shooting step: After performing the step g, the profile camera driving module of the Z-direction moving module on the three-dimensional mechanical transmission device is moved to be directly above the centrifugal filter sandwich cup on the consumable storage device, the image of the multiple stained films is shot and uploaded to the computer display screen for analysis;

[0085] j. Heating step: After performing the step i, the electric clamp jaw in the Z-direction moving module on the three-dimensional mechanical transmission device clamps the centrifugal filter sandwich cup on the consumable storage device, and is moved to be above the heater to heat the multiple stained films, the heating time is 5-10 minutes, and the dried stained film is obtained;

[0086] k. Oil dropping step: After performing the step j, the slide moving support and the slide lifting top rod on the slide preparation mechanical device first move the slide in the slide storage box to the oil dropping position on the slide operation platform, the oil suction electromagnetic valve on the slide operation platform is opened, the microscope oil in the oil injection bottle is injected into the oil injection piston, and then the oil injection electromagnetic valve on the oil dropping device is opened to drop the microscope oil in the oil injection piston through the oil dropping needle head on the slide operation platform to the slide to obtain the oil-dropped slide;

[0087] m. Slide preparation step: After performing the step k, the slide moving support on the slide preparation mechanical device moves the oil-dropped slide to the slide preparation position on the slide operation platform, the electric clamp jaw of the Z-direction moving module on the three-dimensional mechanical transmission device places the dried stained film at the bottom of the clamped centrifugal filter sandwich cup on the oil-dropped slide, the cover glass pump and the cover glass suction valve are opened, the vacuum suction disc on the suction disc connecting platform sucks the cover glass in the cover glass storage box and presses it on the dried stained film in the centrifugal filter sandwich cup, so that it is left between the oil-dropped slide and the cover glass, and the preparation of one sputum smear slide is completed;

[0088] n. The sending step: after the step m is executed, the electric clamp jaw of the Z direction moving module of the three-dimensional mechanical transmission device throws the used centrifugal filter type sandwich cup into the garbage can, and moves the slide glass operation platform, slide glass moving support and slide glass collecting device in the slide glass collecting device, and sends the sputum smear slide glass into the slide glass collecting device;

[0089] p. Repeat steps j, k, m and n to complete the preparation of sputum smear slide glasses from the multiple dyeing films at the bottom of each centrifugal filter type sandwich cup, and finally move the slide glass collecting device out of the system to obtain a batch of sputum smear slide glasses.

[0090] The beneficial effects of the present application are:

[0091] The present application realizes the automatic sputum homogenization, dyeing and slide glass preparation process, and integrates the three steps of preparing sputum smear slide glasses. The automatic process of the system replaces manual operation, avoids direct contact of the test personnel with the sputum sample, and effectively prevents infection of the test personnel and occurrence of iatrogenic pollution.

[0092] The present application can simultaneously process a batch of sputum in 20 sputum cups, or process sputum in a certain sputum cup alone, thereby improving the preparation efficiency of sputum smear slide glasses, shortening the processing time of sputum samples, and meeting the processing needs of a small amount or multiple sputum samples.

[0093] During the injection of the digestive solution and sampling, the present application uses a digestive solution injection pipeline to extract the digestive solution pipettor and the liquefied sputum pipettor, so that the integration degree is higher.

[0094] During the sample homogenization process, the present application oscillates the sputum cup after the digestive solution is injected in a clockwise or counterclockwise direction, which can better homogenize and release the bacteria in the sputum, and facilitate the subsequent dyeing process.

[0095] During the dyeing process, the present application integrates 3-5 kinds of dyeing solution to meet the use of different kinds of dyeing solution for homogenized sputum, and performs a more systematic dyeing process. The present application uses a centrifugal filter type sandwich cup to store the homogenized sputum, and filters the dyeing solution after each use to obtain a dyeing film.

[0096] During the dyeing image shooting process, the present application is provided with a contour camera driving module, which can shoot the dyeing effect image of the dyeing film after the dyeing process and upload the detection, thereby better ensuring the dyeing quality of the sputum smear.

[0097] In the process of making, the application adopts the making mechanical device, the oil dropping device and the Z direction moving module of the three-dimensional mechanical transmission device to automatically drop oil, cover film and cover the slide, complete the making of the sputum smear slide, and make the mechanical automation degree higher.

[0098] The application integrates the slide storage box and the cover glass storage box in the making mechanical device, which is convenient for batch making of the sputum smear slide.

[0099] In the process of sending the slide, the application can automatically place the completed sputum smear slide on the slide collection rack and move it to the outside, which is convenient for subsequent scanning and observation of the sputum smear slide by medical staff.

[0100] The disposable consumables used in the process of injecting digestive fluid, sampling, sample homogenization and dyeing include a digestive fluid pipettor, a sputum cup, a liquefied sputum pipettor and a centrifugal filter sandwich cup, so that the process of making the sputum smear slide is more sanitary and reduces iatrogenic pollution. The application is provided with a garbage can, which can recycle the used disposable consumables.

[0101] The rack of the application is provided with an ultraviolet lamp, which can disinfect the inside of the system; the system further includes a computer display screen connected with the controller in the system, so that the user can realize the operation of the controller through the interaction with the computer display screen.

[0102] The electric part of the system includes an inductive switch and an inductor baffle, which can effectively limit the movement range of each motor in the system and realize accurate control. BRIEF DESCRIPTION OF DRAWINGS

[0103] Figure 1 It is a structural schematic diagram of the rack of the application;

[0104] Figure 2 It is a structural schematic diagram of the front side of the system of the application;

[0105] Figure 3 It is a structural schematic diagram of the rear side of the system of the application;

[0106] Figure 4 It is a structural schematic diagram of the front side of the Z direction moving module in the three-dimensional mechanical transmission device of the application;

[0107] Figure 5 It is a structural schematic diagram of the rear side of the Z direction moving module in the three-dimensional mechanical transmission device of the application;

[0108] Figure 6 It is a structural schematic diagram of the dyeing reagent input device of the application;

[0109] Figure 7 It is a structural schematic diagram of the consumable storage device of the application;

[0110] Figure 8 Fig. 1 is a structural schematic diagram of the sputum homogenizing device of the present application;

[0111] Figure 9 Fig. 2 is a structural schematic diagram of the oil dripping device of the present application;

[0112] Figure 10 Fig. 3 is a structural schematic diagram of the front side of the tabletting machine device of the present application;

[0113] Figure 11 Fig. 4 is a structural schematic diagram of the rear side of the tabletting machine device of the present application;

[0114] Figure 12 Fig. 5 is a structural schematic diagram of the glass slide collecting device of the present application. Embodiment

[0115] The technical solutions in the embodiments of the present application will be clearly and completely described in combination with the accompanying drawings in the embodiments of the present application. Embodiment

[0116] The present embodiment provides a full-automatic sputum staining tabletting system.

[0117] Referring to Figs. 1-5, Figure 1 Figure 2 and Figure 3 , the system comprises a rack 1, a three-dimensional mechanical transmission device 2, a sputum homogenizing device 4, a consumable storage device 3, a tabletting machine device 78, a glass slide collecting device 73, an oil dripping device 90, a staining reagent input device 129 and a controller 15; the faceplate of the rack 1 is provided with a computer display screen 117, the faceplate is provided with an ultraviolet lamp 128, and the bottom of the rack 1 is provided with a garbage can 130. The computer display screen 117 is connected with the controller 15 through a serial port, and the user can interact with the computer display screen 117 to operate the controller 15 in real time; the ultraviolet lamp 128 is used for disinfecting the contaminated areas inside the system; and the garbage can 130 is used for storing the used disposable consumables.

[0118] Referring to Figs. 1-5, Figure 2 Figure 3 , the three-dimensional mechanical transmission device 2 is composed of an X-direction moving module 7, a Y-direction moving module 12 and a Z-direction moving module 32.

[0119] Referring to Figs. 1-5, Figure 2 Figure 3 ​​​The X-direction moving module 7 comprises an X-direction slider 5, an X-direction stepping motor 6 and an X-direction slide 9. The X-direction stepping motor 6 drives the X-direction slider 5 to run on the X-direction slide 9. The X-direction slide 9 is provided with an X-direction photoelectric sensor switch 13, and the X-direction slider 5 is provided with an X-direction sensor stopper 14. The X-direction photoelectric sensor switch 13 and the X-direction sensor stopper 14 determine the zero position of the X-direction slider 5.

[0120] Referring to Figure 2 and Figure 3 , the Y-direction moving module 12 comprises a Y-direction slider 8, a Y-direction stepping motor 10 and a Y-direction slide 11. The Y-direction stepping motor 10 drives the Y-direction slider 8 to run on the Y-direction slide 11. The Y-direction slide 11 is provided with a Y-direction photoelectric sensor switch 17, and the Y-direction slider 8 is provided with a Y-direction sensor stopper 18 and a suction pump 25. The Y-direction photoelectric sensor switch 17 and the Y-direction sensor stopper 18 determine the zero position of the Y-direction slider 8.

[0121] Referring to Figure 2 and Figure 3 , the suction pump 25 is provided with a suction piston 31 and a suction pump photoelectric sensor switch 27. The suction piston 31 is provided with a suction pump sensor stopper 28. The suction pump photoelectric sensor switch 27 and the suction pump sensor stopper 28 determine the zero position of the suction piston 31.

[0122] Referring to Figure 4 and Figure 5 , the Z-direction moving module 32 comprises a Z-direction connecting platform 16, a Z-direction first slide 21, a Z-direction second slide 34, an electric claw stepping motor 35, an electric claw motor slider 38, a pipetting stepping motor 19 and a pipetting motor slider 20. The Z-direction first slide 21 and the Z-direction second slide 34 are arranged below the Z-direction connecting platform 16. The electric claw stepping motor 35 and the pipetting stepping motor 19 are arranged on the Z-direction connecting platform 16. The electric claw stepping motor 35 drives the electric claw motor slider 38 to run on the Z-direction first slide 21. The pipetting stepping motor 19 drives the pipetting motor slider 20 to run on the Z-direction second slide 34.

[0123] Referring to Figure 1 , Figure 2 and Figure 3 , the three-dimensional mechanical transmission device 2 is arranged on the top of the rack 1 through the X-direction slide 9 of the X-direction moving module 7. The Y-direction slide 11 of the Y-direction moving module 12 is arranged on the X-direction slider 5. The Z-direction connecting platform 16 of the Z-direction moving module 32 is arranged on the Y-direction slider 8.

[0124] Referring to Figure 8The sputum homogenizing device 4 is composed of a driving base 42, a shaking platform 44, a shaking stepping motor 45, a crankshaft 43 and a shaking motor belt driving wheel 46. The shaking stepping motor 45 is arranged on the driving base 42. The crankshaft 43 is four pieces and is hinged to the driving base 42. The shaking stepping motor 45 drives the four crankshafts 43 to rotate synchronously through the shaking motor belt driving wheel 46. The shaking platform 44 is hinged to the crankshafts 43. The shaking motor belt driving wheel 46 is provided with an inductor baffle 47 of a shaking motor. The inner side of the driving base 42 is provided with a photoelectric inductive switch 41 of the shaking motor. The photoelectric inductive switch 41 of the shaking motor and the inductor baffle 47 of the shaking motor determine the zero position and the maximum position of the shaking platform 44. The shaking platform 44 is provided with a plurality of sputum cups 122. The sputum homogenizing device 4 is arranged at the bottom of the rack 1 through the driving base 42.

[0125] Referring to Figure 7 The consumable storage device 3 is composed of a consumable platform frame 48, a container storage disc 49, a heater 124, a liquid pumping electromagnetic valve 50 and a liquid pumping pump 51. The liquid pumping electromagnetic valve 50 is a plurality of pieces and is distributed around the consumable platform frame 48. The heater 124 is arranged on one side of the consumable platform frame 48. The liquid pumping pump 51 is arranged beside the consumable platform frame 48. The container storage disc 49 is arranged above the consumable platform frame 48. A plurality of centrifugal filter type sandwich cups 120, a plurality of liquefied sputum pipettors 121 and a plurality of digestive liquid pipettors 123 are sequentially arranged on the container storage disc 49. The consumable storage device 3 is arranged at the bottom of the rack 1 through the consumable platform frame 48.

[0126] Referring to Figure 10 and Figure 11 The slide making mechanical device 78 is composed of a slide making machine support 67, a slide operation platform 83 and a Z direction operation platform stepping motor 77.

[0127] Referring to Figure 10 and Figure 11 The slide making machine support 67 is provided with a Z direction slide making slide 87 and a Z direction slide making slide block 108. The Z direction operation platform stepping motor 77 drives the Z direction slide making slide block 108 to run on the Z direction slide making slide 87. The slide operation platform 83 is arranged on the Z direction slide making slide block 108. The bottom end of the slide making machine support 67 is provided with an operation platform photoelectric inductive switch 79. The Z direction slide making slide block 108 is provided with an operation platform inductor baffle 80. The operation platform photoelectric inductive switch 79 and the operation platform inductor baffle 80 determine the zero position of the slide operation platform 83.

[0128] Referring to Figure 10 and Figure 11The slide operation platform 83 is provided with a suction disc connecting platform 112 and a suction disc stepping motor 107; the slide operation platform 83 is provided with a Z-direction suction disc slide 81 and a Z-direction suction disc slide block, the suction disc stepping motor 107 drives the Z-direction suction disc slide block to run on the Z-direction suction disc slide 81, and the suction disc connecting platform 112 is arranged on the Z-direction suction disc slide block; the Z-direction suction disc slide 81 of the slide operation platform 83 is provided with a suction disc platform photoelectric sensing switch 109, and the suction disc connecting platform 112 is provided with a suction disc platform sensor baffle 110; the suction disc platform photoelectric sensing switch 109 and the suction disc platform sensor baffle 110 determine the zero position of the suction disc connecting platform 112.

[0129] Referring to Figure 10 and Figure 11 The suction disc connecting platform 112 is provided with a suction disc rotating stepping motor 111 and a suction disc rotating arm 113, the suction disc rotating stepping motor 111 drives the suction disc rotating arm 113 to swing on the suction disc connecting platform 112; the rear side of the suction disc rotating stepping motor 111 is provided with a suction disc rotating arm photoelectric sensing switch 118, and one end of the suction disc rotating arm 113 is provided with a suction disc rotating arm sensor baffle 119; the suction disc rotating arm photoelectric sensing switch 118 and the suction disc rotating arm sensor baffle 119 determine the zero position of the suction disc rotating arm 113; the suction disc connecting platform 112 is provided with a cover glass suction valve 115 and a cover glass pump 116; the suction disc rotating arm 113 is provided with a vacuum suction disc 114; the cover glass pump 116 on the suction disc connecting platform 112 is connected with the vacuum suction disc 114 through the cover glass suction valve 115.

[0130] Referring to Figure 10 and Figure 11 The slide operation platform 83 is provided with a Y-direction slide stepping motor 86 and a slide moving support 84, the slide moving support 84 is provided with a slide 85, and the Y-direction slide stepping motor 86 drives the slide 85 to move along the Y direction through the slide moving support 84; the slide moving support 84 is provided with a slide Y-direction sensor baffle 89, and one end of the Y-direction slide stepping motor 86 is provided with a slide Y-direction photoelectric sensing switch 88; the slide Y-direction photoelectric sensing switch 88 and the slide Y-direction sensor baffle 89 determine the zero position of the slide moving support 84.

[0131] Referring to Figure 10 and Figure 11The slide operation platform 83 is provided with a Z-direction slide stepping motor 91 and a slide storage box 82; the slide storage box 82 is provided with a slide lifting jib 92, and the slide storage box 82 is provided with a slide 85; the Z-direction slide stepping motor 91 drives the slide lifting jib 92 to touch the slide 85 in the slide storage box 82 to move along the Z direction; one end of the slide lifting jib 92 is provided with a storage box sensor stopper 94, and the slide operation platform 83 is provided with a storage box photoelectric sensor switch 93; the storage box photoelectric sensor switch 93 and the storage box sensor stopper 94 determine the zero position of the slide lifting jib 92.

[0132] Referring to Figure 10 and Figure 11 , the slide operation platform 83 is further provided with an oil suction electromagnetic valve 65, a cover glass storage box 105 and a oil dropping needle 103; the cover glass storage box 105 is provided with a cover glass 106; the slide making mechanical device 78 is arranged at the bottom of the rack 1 through a slide making machine support 67.

[0133] Referring to Figure 12 , the slide collecting device 73 is composed of an X-direction collecting moving module 68 and a slide collecting rack 70; the X-direction collecting moving module 68 comprises an X-direction collecting sliding block 74, an X-direction collecting stepping motor 71, a collecting motor belt driving wheel 72 and an X-direction collecting slide 69; the X-direction collecting stepping motor 71 drives the X-direction collecting sliding block 74 to run on the X-direction collecting slide 69 through the collecting motor belt driving wheel 72; the slide collecting rack 70 is arranged on the X-direction collecting sliding block 74; the left and right ends of the X-direction collecting slide 69 are provided with a collecting photoelectric sensor switch 75, and the X-direction collecting sliding block 74 is provided with a collecting sensor stopper 76; the collecting photoelectric sensor switch 75 and the collecting sensor stopper 76 determine the zero position and the maximum position of the slide collecting rack 70; the slide collecting device 73 is arranged at the bottom of the rack 1 through the X-direction collecting slide 69.

[0134] Referring to Figure 9 , the oil dropping device 90 is composed of an oil dropping support 96 provided with an oil injection piston 97, an oil dropping stepping motor 95, an oil injection electromagnetic valve 98, an oil dropping motor belt driving wheel 99, an oil injection transmission shaft 100 and an oil dropping motor contact sensor switch 104.

[0135] Referring to Figure 9The oil dripping stepper motor 95 is connected with the oil injection transmission shaft 100 through the oil dripping motor belt driving wheel 99, and the oil injection transmission shaft 100 is connected with the oil injection piston 97; the contact sensing switch 104 of the oil dripping motor is two pieces and is arranged on the upper and lower sides of the oil dripping support 96 respectively; the oil dripping motor sensor shielding block 101 is arranged on the oil injection transmission shaft 100, and the oil dripping motor sensor shielding block 101 moves between the contact sensing switches 104 of the oil dripping motor on the upper and lower sides, so as to determine the zero position and the maximum position of the oil injection piston 97.

[0136] Referring to Figure 9 The oil injection electromagnetic valve 98 and the oil suction electromagnetic valve 65 on the tablet making mechanical device 78 are electrically connected with the oil dripping stepper motor 95; the oil injection piston 97 is provided with an oil inlet nozzle and an oil outlet nozzle; the oil inlet nozzle is connected with the oil suction electromagnetic valve 65 on the tablet making mechanical device 78 and the oil injection bottle 102, and the oil outlet nozzle is connected with the oil injection electromagnetic valve 98 and the oil dripping needle head 103 on the tablet making mechanical device 78; the oil dripping device 90 is arranged at the bottom of the rack 1 through the oil dripping support 96.

[0137] Referring to Figure 6 The staining reagent input device 129 is composed of a staining pump support 125, a staining reagent bottle rack 127 and a two-dimensional mechanical transmission device 131.

[0138] Referring to Figure 6 The staining pump support 125 is provided with a plurality of staining pumps 60 inside the support and a plurality of staining electromagnetic valves 64 outside the support; the staining reagent bottle rack 127 is provided with a plurality of staining reagent bottles 63 and a digestive liquid reagent bottle 126, and the plurality of staining reagent bottles 63 are connected with the plurality of staining pumps 60 and the plurality of staining electromagnetic valves 64 one by one; the staining pump support 125 is further provided with a contact sensing switch 59 in the X direction of staining and a pipette buckle 66; the pipette buckle 66 is used to throw the used liquefied sputum pipette 121 and the digestive liquid pipette 123 into the garbage can 130.

[0139] Referring to Figure 6 The two-dimensional mechanical transmission device 131 is composed of an X direction staining moving module 132 and a Y direction staining moving module 57.

[0140] Referring to Figure 6 The X direction staining moving module 132 comprises an X direction staining sliding block 52, an X direction staining stepper motor 53 and an X direction staining slide 54; the X direction staining stepper motor 53 drives the X direction staining sliding block 52 to run on the X direction staining slide 54; the X direction staining sliding block 52 is provided with a sensor blocking piece 61 in the X direction of staining; the sensor blocking piece 61 in the X direction of staining and the contact sensing switch 59 in the X direction of staining on the staining pump support 125 determine the zero position of the X direction staining sliding block 52.

[0141] Referring to Figure 6 , the Y-direction dyeing moving module 57 comprises a Y-direction dyeing slider 58, a Y-direction dyeing stepping motor 56 and a Y-direction dyeing slide 55; the Y-direction dyeing stepping motor 56 drives the Y-direction dyeing slider 58 to run on the Y-direction dyeing slide 55; the Y-direction dyeing slide 55 of the Y-direction dyeing moving module 57 is arranged on the X-direction dyeing slider 52; the Y-direction dyeing slider 58 is provided with a dropper 133; the Y-direction dyeing slide 55 is provided with a contact sensing switch 62 in the Y-direction; and the contact sensing switch 62 in the Y-direction determines the zero position of the dropper 133.

[0142] Referring to Figure 6 , the dyeing reagent input device 129 is sequentially arranged at the bottom of the rack 1 through the dyeing pump support 125, the dyeing reagent bottle rack 127 and the X-direction dyeing slide 54 of the two-dimensional mechanical transmission device 131.

[0143] Referring to Figure 1 , Figure 2 and Figure 3 , the controller 15 is a PLC programmable controller, and the controller 15 is electrically connected with the computer display screen 117 of the rack 1 and the electrical parts of the three-dimensional mechanical transmission device 2, the sputum homogenizing device 4, the consumable storage device 3, the tablet making mechanical device 78, the glass slide collecting device 73, the oil dropping device 90 and the dyeing reagent input device 129.

[0144] Referring to Figure 4 and Figure 5 , the Z-direction moving module 32 is provided with an electric claw motor slider 38, an electric claw 37 on the electric claw motor slider 38 and a semicircular arc clamp 36 on the electric claw 37; the Z-direction moving module 32 is provided with a digestive liquid injection pipeline 22 on the pipette motor slider 20; the side pipeline of the digestive liquid injection pipeline 22 is connected with a digestive liquid pump 24; the Z-direction first slide 21 is provided with a photoelectric sensing switch 39 of the electric claw motor and an inductor baffle 40 of the electric claw motor on the electric claw motor slider 38; the Z-direction second slide 34 is provided with a photoelectric sensing switch 29 of the pipette motor and an inductor baffle 30 of the pipette motor on the pipette motor slider 20; and the photoelectric sensing switch 39 of the electric claw motor and the inductor baffle 40 of the electric claw motor determine the zero position of the electric claw 37, and the photoelectric sensing switch 29 of the pipette motor and the inductor baffle 30 of the pipette motor determine the zero position of the digestive liquid injection pipeline 22.

[0145] Referring to Figure 4 and Figure 5The Z-direction connecting platform 16 on the Z-direction moving module 32 is provided with a digestive fluid electromagnetic valve 23, a liquid suction electromagnetic valve 26 and a profile camera driving module 33. The digestive fluid electromagnetic valve 23 is electrically connected with a digestive fluid pump 24, and the liquid suction pump 25 is electrically connected with the liquid suction electromagnetic valve 26.

[0146] Referring to Figure 7 The bottom of the plurality of centrifugal filter sandwich cups 120 is connected with the plurality of liquid suction electromagnetic valves 50 through liquid pipes respectively, and the liquid suction pump 51 is electrically connected with the liquid suction electromagnetic valve 50. Embodiment

[0147] The embodiment provides a full-automatic sputum staining and sheet making method according to the above full-automatic sputum staining and sheet making system. The method specifically implements the following steps:

[0148] a. Consumable taking and placing step: referring to Figure 1 , Figure 6 , Figure 7 , Figure 8 , Figure 9 and Figure 10 Before the system starts to work, the remaining vessels in the garbage can 130 are emptied, 20 sputum cups 122 are placed in the sputum homogenizing device 4, 20 liquefied sputum pipettors 121, 20 centrifugal filter sandwich cups 120 and 20 digestive fluid pipettors 123 are placed in the consumable storage device 3, 50 glass slides 85 and 50 cover glasses 106 are placed in the sheet making mechanical device 78, the microscope oil is added in the oil injection bottle 102, and five kinds of staining reagents and digestive fluid are respectively injected into the five staining reagent bottles 63 and the digestive fluid reagent bottle 126 on the staining reagent bottle rack 127.

[0149] b. Resetting step: referring to Figure 1 After the step a is performed, the system is started, and the equipment is reset by the operation controller 15.

[0150] c. Digestive fluid injection step: referring to Figure 2 , Figure 5 , Figure 7 , Figure 6 and Figure 8 After the step b is performed, the digestive fluid injection pipeline 22 of the Z-direction moving module 32 on the three-dimensional mechanical transmission device 2 clamps and lifts the digestive fluid pipettor 123 on the consumable storage device 3, the digestive fluid pump 24 and the digestive fluid electromagnetic valve 23 are turned on, the digestive fluid in the digestive fluid reagent bottle 126 on the staining reagent input device 129 is injected into the sputum cup 122 on the sputum homogenizing device 4 through the digestive fluid pipettor 123, and then the digestive fluid pipettor 123 is thrown into the garbage can 130. The above steps are repeated to complete the injection of each sputum cup 122 in the step a.

[0151] d. Sample homogenization step: referring toFigure 8 After the step c is performed, the oscillation stepping motor 45 on the sputum homogenization device 4 drives the sputum cup 122 on the oscillation platform 44 to oscillate clockwise or counterclockwise, and the oscillation time is 5 minutes to obtain the homogenized sputum.

[0152] e. Sampling step: refer to Figure 2 , Figure 4 , Figure 5 , Figure 7 and Figure 8 After the step d is performed, the digestive juice injection pipeline 22 of the Z-direction moving module 32 on the three-dimensional mechanical transmission device 2 clutches and lifts the liquefied sputum pipette 121 on the consumable storage device 3, opens the suction pump 25 and the suction solenoid valve 26, and sucks the homogenized sputum in the sputum cup 122 on the sputum homogenization device 4 into the liquefied sputum pipette 121; at this time, the digestive juice injection pipeline 22 transfers the homogenized sputum to the centrifugal filter type sandwich cup 120 on the consumable storage device 3; and then the liquefied sputum pipette 121 is thrown into the garbage can 130. The sampling of the homogenized sputum in each sputum cup 122 in step d is completed by circulation.

[0153] f. Suction step: refer to Figure 7 After the step e is performed, the suction solenoid valve 50 and the suction pump 51 on the consumable storage device 3 are opened, and the excess homogenized sputum in the centrifugal filter type sandwich cup 120 is sucked out, and the suction time is 1 minute. The bacteria in the homogenized sputum adhere to the bottom of the centrifugal filter type sandwich cup 120, and the sputum film is obtained.

[0154] g. Staining step: refer to Figure 6 and Figure 7 After the step f is performed, the dropper 133 of the two-dimensional mechanical transmission device 131 on the staining reagent input device 129 moves above the centrifugal filter type sandwich cup 120 on the consumable storage device 3, the staining pump 60 and the staining solenoid valve 64 are opened, the staining liquid in one staining reagent bottle 63 is dropped into the sputum film of the centrifugal filter type sandwich cup 120, and is left for 5 minutes to complete the staining, and the staining film is obtained from the bottom of the centrifugal filter type sandwich cup 120.

[0155] h. Repeat steps f and g, and sequentially complete the staining of the staining film by 5 kinds of staining liquids in 5 staining reagent bottles 63 to obtain multiple staining films.

[0156] i. Staining image shooting step: refer to Figure 1 , Figure 2 , Figure 4 and Figure 7After the step g is executed, the profile camera driving module 33 of the Z direction moving module 32 on the three-dimensional mechanical transmission device 2 moves to the top of the centrifugal filter sandwich cup 120 on the consumable storage device 3, images of the multiple dyed films are taken and uploaded to the computer display screen 117 for analysis.

[0157] j. Heating step: refer to Figure 2 , Figure 4 and Figure 7 After the step i is executed, the electric clamp jaw 37 in the Z direction moving module 32 on the three-dimensional mechanical transmission device 2 clamps the centrifugal filter sandwich cup 120 on the consumable storage device 3, and moves to the top of the heater 124 to heat the multiple dyed films, the heating time is 5 minutes, and the dried dyed film is obtained.

[0158] k. Oil dropping step: refer to Figure 9 , Figure 10 and Figure 11 After the step j is executed, the slide moving support 84 and the slide lifting top rod 92 in the slide preparation mechanical device 78 first move the slide 85 in the slide storage box 82 to the oil dropping position on the slide operation platform 83, open the oil suction electromagnetic valve 65 on the slide operation platform 83, inject the microscope oil in the oil injection bottle 102 into the oil injection piston 97, and then open the oil injection electromagnetic valve 98 on the oil dropping device 90 to drop the microscope oil in the oil injection piston 97 through the oil dropping needle 103 on the slide operation platform 83 to the slide 85, and obtain the oil-dropped slide 85.

[0159] m. Slide preparation step: refer to Figure 2 , Figure 4 , Figure 10 and Figure 11 After the step k is executed, the slide moving support 84 in the slide preparation mechanical device 78 moves the oil-dropped slide 85 to the slide preparation position on the slide operation platform 83, the electric clamp jaw 37 of the Z direction moving module 32 on the three-dimensional mechanical transmission device 2 clamps the dried dyed film at the bottom of the centrifugal filter sandwich cup 120 and places it on the oil-dropped slide 85, and then opens the cover glass pump 116 and the cover glass suction valve 115, the vacuum suction disc 114 on the suction disc connecting platform 112 sucks the cover glass 106 in the cover glass storage box 105 and presses it on the dried dyed film in the centrifugal filter sandwich cup 120, so that it remains between the oil-dropped slide 85 and the cover glass 106, and the preparation of a piece of sputum smear slide is completed.

[0160] n. Slide sending step: refer to Figure 2 , Figure 4 , Figure 10 and Figure 12After the step m is executed, the electric gripper 37 of the Z direction moving module 32 of the three-dimensional mechanical transmission device 2 throws the used centrifugal filter sandwich cup 120 into the garbage can 130, and moves the slide operation platform 83, the slide moving support 84 and the slide collection rack 70 in the slide collection device 73 in the slide making mechanical device 78, and sends the sputum smear slide into the slide collection rack 70.

[0161] p.The steps j, k, m and n are repeated to sequentially complete the preparation of the sputum smear slide by using the multiple staining films at the bottom of each centrifugal filter sandwich cup 120, and finally the slide collection rack 70 is moved out of the system to obtain a batch of sputum smear slides.

[0162] The present application converts the current manual homogenization, staining and slide making process of sputum into an operation using a fully automatic system, reduces the current labor cost and time cost of sputum smear slide preparation, improves the efficiency while ensuring the quality of sputum smear slide preparation. The above describes the basic principles, main features and advantages of the two examples of the present application.

[0163] Finally, it should be noted that the above examples are only a preferred solution of the present application, and the present application is not limited to the specific embodiments described above. The skilled in the art can also change and modify the above embodiments. Those skilled in the art should understand that the technical solutions recorded in the foregoing examples can be modified, or some technical features can be replaced equivalently without departing from the purpose, principles and scope of the technical solutions of the present application. Any modification, replacement or improvement should be included in the protection scope and the scope of the claims of the present application.

Claims

1. A fully automated sputum staining and slide preparation system, characterized in that, The system includes a frame (1), a three-dimensional mechanical transmission device (2), a sputum homogenization device (4), a consumable storage device (3), a slide preparation device (78), a slide collection device (73), an oil dripping device (90), a staining reagent input device (129), and a controller (15). The rack (1) has a computer display screen (117) on its panel, an ultraviolet lamp (128) inside the panel, and a trash can (130) at the bottom of the rack (1). The three-dimensional mechanical transmission device (2) consists of an X-direction moving module (7), a Y-direction moving module (12), and a Z-direction moving module (32). The X-direction movement module (7) includes: an X-direction slider (5), an X-direction stepper motor (6), and an X-direction slide rail (9); the X-direction stepper motor (6) drives the X-direction slider (5) to run on the X-direction slide rail (9); The X-direction slide (9) is provided with an X-direction photoelectric sensor switch (13) and the X-direction slider (5) is provided with an X-direction sensor baffle (14). The Y-direction moving module (12) includes: a Y-direction slider (8), a Y-direction stepper motor (10), and a Y-direction slide rail (11). The Y-direction stepper motor (10) drives the Y-direction slider (8) to run on the Y-direction slide rail (11); The Y-direction slide (11) is provided with a Y-direction photoelectric sensor switch (17), and the Y-direction slider (8) is provided with a Y-direction sensor baffle (18) and a suction pump (25). The suction pump (25) is equipped with a suction piston (31) and a photoelectric sensor switch (27) for the suction pump. The suction piston (31) is equipped with a sensor baffle (28) for the suction pump. The Z-direction moving module (32) includes: a Z-direction connecting platform (16), a Z-direction first slide rail (21), a Z-direction second slide rail (34), an electric gripper stepper motor (35), an electric gripper motor slider (38), a pipetting stepper motor (19), and a pipetting motor slider (20). The first slide rail (21) and the second slide rail (34) in the Z direction are located below the connecting platform (16) in the Z direction; The electric gripper stepper motor (35) and the liquid transfer stepper motor (19) are mounted on the Z-direction connecting platform (16); The electric gripper stepper motor (35) drives the electric gripper motor slider (38) to run on the first slide rail (21) in the Z direction; The pipetting stepper motor (19) drives the pipetting motor slider (20) to run on the second slide rail (34) in the Z direction; The three-dimensional mechanical transmission device (2) is located on the top of the frame (1) via the X-direction slide (9) of the X-direction moving module (7); the Y-direction slide (11) of the Y-direction moving module (12) is located on the X-direction slider (5); and the Z-direction connecting platform (16) of the Z-direction moving module (32) is located on the Y-direction slider (8). The Z-direction moving module (32) has an electric gripper (37) on its electric gripper motor slider (38), and the electric gripper (37) has a semi-circular arc gripper (36). The Z-direction moving module (32) has a digestive fluid injection pipe (22) on its pipetting motor slider (20); the side pipe on the digestive fluid injection pipe (22) is connected to the digestive fluid pump (24). The sputum homogenization device (4) consists of a drive base (42), an oscillation platform (44), an oscillation stepper motor (45), a crankshaft (43), and an oscillation motor belt drive wheel (46); The oscillating stepper motor (45) is mounted on the drive base (42), and the crankshaft (43) consists of four parts, which are hinged to the drive base (42). The oscillating stepper motor (45) drives the four crankshafts (43) to rotate synchronously via the oscillating motor belt drive wheel (46). The oscillating platform (44) is hinged to the crankshaft (43). The vibrating motor belt drive pulley (46) is provided with a sensor baffle (47) for the vibrating motor, and the inner side of the drive base (42) is provided with a photoelectric sensor switch (41) for the vibrating motor. The oscillation platform (44) is equipped with several sputum cups (122). The sputum homogenizing device (4) is mounted on the bottom of the frame (1) via a drive base (42); The consumable storage device (3) consists of a consumable platform frame (48), a container storage tray (49), a heater (124), a liquid extraction solenoid valve (50), and a liquid extraction pump (51); Several solenoid valves (50) are distributed around the consumable platform frame (48). A heater (124) is located on one side of the consumable platform frame (48). A pump (51) is located next to the consumable platform frame (48). A container storage tray (49) is located above the consumable platform frame (48). Several centrifugal filter jacketed cups (120), several liquefied sputum pipettes (121), and several digestive fluid pipettes (123) are placed on the container storage tray (49) in sequence. The consumable storage device (3) is mounted on the bottom of the rack (1) via a consumable platform frame (48); The slide preparation machine (78) consists of a slide preparation machine support (67), a slide operation platform (83), and a Z-direction operation platform stepper motor (77); The slide preparation machine support (67) is provided with a Z-direction slide preparation slide (87) and a Z-direction slide preparation slider (108). The Z-direction operation platform stepper motor (77) drives the Z-direction slide preparation slider (108) to run on the Z-direction slide preparation slide (87). The slide operation platform (83) is located on the Z-direction slide preparation slider (108). The bottom of the film-making machine bracket (67) is provided with an operating platform photoelectric sensor switch (79), and the Z-direction film-making slider (108) is provided with an operating platform sensor baffle (80). The slide operation platform (83) is equipped with a suction cup connection platform (112) and a suction cup stepper motor (107). The slide operation platform (83) is provided with a Z-direction suction cup slide (81) and a Z-direction suction cup slider. The suction cup stepper motor (107) drives the Z-direction suction cup slider to run on the Z-direction suction cup slide (81). The suction cup connecting platform (112) is located on the Z-direction suction cup slider. The suction cup connection platform (112) is equipped with a cover glass suction valve (115) and a cover glass pump (116). The slide operation platform (83) is provided with a suction cup platform photoelectric sensor switch (109) on the Z-direction suction cup slide (81) and a suction cup platform sensor baffle (110) on the suction cup connection platform (112). The suction cup connection platform (112) is equipped with a suction cup rotating stepper motor (111) and a suction cup rotating arm (113). The suction cup rotating stepper motor (111) drives the suction cup rotating arm (113) to swing on the suction cup connection platform (112). The suction cup rotating stepper motor (111) is provided with a suction cup rotating arm photoelectric sensor switch (118) on the rear side, and a suction cup rotating arm sensor baffle (119) is provided at one end of the suction cup rotating arm (113). The suction cup rotating arm (113) is equipped with a vacuum suction cup (114); the suction cup connecting platform (112) is equipped with a cover glass pump (116) which is connected to the vacuum suction cup (114) via a cover glass suction valve (115); The slide operation platform (83) is provided with a Y-direction slide stepper motor (86) and a slide moving bracket (84). A slide (85) is provided on the slide moving bracket (84). The Y-direction slide stepper motor (86) touches the slide (85) through the slide moving bracket (84) to move it along the Y direction. The slide moving support (84) is provided with a slide Y-direction sensor baffle (89), and one end of the Y-direction slide stepper motor (86) is provided with a slide Y-direction photoelectric sensor switch (88). The slide operation platform (83) is equipped with a Z-direction slide stepper motor (91) and a slide storage box (82); the slide storage box (82) is equipped with a slide lifting rod (92), and a slide (85) is stored inside the slide storage box (82). The Z-direction slide stepper motor (91) touches the slide (85) inside the slide storage box (82) via the slide lifting rod (92) to move it along the Z direction; One end of the slide lifting rod (92) is provided with a storage box sensor baffle (94), and the slide operation platform (83) is provided with a storage box photoelectric sensor switch (93). The slide operation platform (83) is also equipped with an oil suction solenoid valve (65), a cover slide storage box (105), and an oil dripping needle (103). The cover glass storage box (105) contains a cover glass (106). The film-making machine device (78) is mounted on the bottom of the frame (1) via the film-making machine bracket (67); The slide collecting device (73) consists of an X-direction collecting and moving module (68) and a slide collecting rack (70); The X-direction collecting and moving module (68) includes: an X-direction collecting slider (74), an X-direction collecting stepper motor (71), a collecting motor belt drive wheel (72), and an X-direction collecting slide (69); the X-direction collecting stepper motor (71) drives the X-direction collecting slider (74) to run on the X-direction collecting slide (69) via the collecting motor belt drive wheel (72); The slide collection rack (70) is mounted on the X-direction collection slider (74); The left and right ends of the X-direction collecting slide (69) are provided with a collecting photoelectric sensor switch (75) and the X-direction collecting slider (74) is provided with a collecting sensor baffle (76). The slide collecting device (73) is located at the bottom of the frame (1) via the X-direction collecting slide (69); The oil dripping device (90) consists of an oil dripping bracket (96) equipped with an oil injection piston (97), an oil dripping stepper motor (95), an oil injection solenoid valve (98), an oil dripping motor belt drive wheel (99), an oil injection transmission shaft (100), and a contact sensing switch (104) for the oil dripping motor. The oil-driving stepper motor (95) is connected to the oil-injection drive shaft (100) via the oil-driving motor belt drive wheel (99), and the oil-injection drive shaft (100) is connected to the oil-injection piston (97). The oil-driving motor has two contact sensing switches (104), which are respectively located on the upper and lower sides of the oil-driving bracket (96). The oil-filling transmission shaft (100) is provided with a sensor blocking block (101) for the oil-driving motor. The sensor blocking block (101) for the oil-driving motor moves between the contact sensing switches (104) on the upper and lower sides of the oil-driving motor. The oil injection solenoid valve (98) and the oil suction solenoid valve (65) on the film-making machine (78) are electrically connected to the oil dripping stepper motor (95); The oil injection piston (97) is provided with an oil inlet and an oil outlet. The oil inlet is connected to the oil suction solenoid valve (65) and the oil injection bottle (102) on the film making machine (78). The oil outlet is connected to the oil injection solenoid valve (98) and the oil dripping needle (103) on the film making machine (78). The oil dripping device (90) is mounted on the bottom of the frame (1) via an oil dripping bracket (96); The staining reagent input device (129) consists of a staining pump support (125), a staining reagent bottle rack (127), and a two-dimensional mechanical transmission device (131); The dyeing pump bracket (125) has several dyeing pumps (60) inside the bracket and several dyeing solenoid valves (64) outside the bracket. The staining reagent bottle rack (127) contains several staining reagent bottles (63) and digestion solution reagent bottles (126). The several staining reagent bottles (63) are respectively connected to several staining pumps (60) and several staining solenoid valves (64). The staining pump bracket (125) is also equipped with a staining X-direction contact sensor switch (59) and a pipette clip (66). The two-dimensional mechanical transmission device (131) consists of an X-direction dyeing movement module (132) and a Y-direction dyeing movement module (57); The X-direction dyeing moving module (132) includes: an X-direction dyeing slider (52), an X-direction dyeing stepper motor (53), and an X-direction dyeing slide (54); the X-direction dyeing stepper motor (53) drives the X-direction dyeing slider (52) to run on the X-direction dyeing slide (54); The Y-direction dyeing moving module (57) includes: a Y-direction dyeing slider (58), a Y-direction dyeing stepper motor (56), and a Y-direction dyeing slide (55); the Y-direction dyeing stepper motor (56) drives the Y-direction dyeing slider (58) to run on the Y-direction dyeing slide (55); The Y-direction dyeing slide (55) of the Y-direction dyeing moving module (57) is located on the X-direction dyeing slider (52); The Y-direction staining slider (58) is equipped with a dropper (133); The X-direction dyeing slider (52) is provided with a sensor baffle (61) for dyeing the X direction. The dyeing slide (55) in the Y direction is provided with a contact sensing switch (62) in the dyeing Y direction. The dyeing reagent input device (129) is sequentially located at the bottom of the frame (1) via the dyeing pump bracket (125), the dyeing reagent bottle rack (127), and the X-direction dyeing slide (54) of the two-dimensional mechanical transmission device (131); The controller (15) is a PLC programmable controller. The controller (15) is electrically connected to the computer display screen (117) of the frame (1) and the electrical parts of the three-dimensional mechanical transmission device (2), sputum homogenization device (4), consumable storage device (3), slide preparation device (78), glass slide collection device (73), oil dripping device (90) and staining reagent input device (129).

2. The fully automated sputum staining and slide preparation system according to claim 1, characterized in that, The Z-direction moving module (32) has a photoelectric sensor switch (39) for an electric claw motor at the top of the first slide rail (21) and a sensor baffle (40) for an electric claw motor on the slider (38) of the electric claw motor. The Z-direction second slide rail (34) has a photoelectric sensor switch (29) for a pipetting motor at the top of the top and a sensor baffle (30) for a pipetting motor on the slider (20) of the pipetting motor. The Z-direction moving module (32) is equipped with a digestive fluid solenoid valve (23), a suction solenoid valve (26) and a contour camera driving module (33) on the Z-direction connecting platform (16). The digestive fluid solenoid valve (23) is electrically connected to the digestive fluid pump (24), and the suction pump (25) is electrically connected to the suction solenoid valve (26).

3. The fully automated sputum staining and slide preparation system according to claim 1, characterized in that, The bottom of the several centrifugal filter jacketed cups (120) is connected to several liquid extraction solenoid valves (50) via liquid pipes, and the liquid extraction pump (51) is electrically connected to the liquid extraction solenoid valves (50).

4. A fully automated method for sputum staining and slide preparation, characterized in that, This method uses the fully automated sputum staining and slide preparation system described in claims 1-3, and the specific implementation steps are as follows: a. Consumables handling procedure: Before the system starts working, empty the containers left in the trash can (130), and place 1 to 20 sputum cups (122) in the sputum homogenizer (4); place 1 to 20 liquefied sputum pipettes (121), 1 to 20 centrifugal filter jacketed cups (120), and 1 to 20 digestive fluid pipettes (123) in the consumables storage device (3); place 1 to 50 slides (85) and 1 to 50 coverslips (106) in the slide preparation device (78); add microscope oil to the oil bottle (102); and inject 3 to 5 kinds of staining solutions and digestive solutions into 3 to 5 staining reagent bottles (63) and digestive fluid reagent bottles (126) on the staining reagent bottle rack (127), respectively. b. Reset procedure: After performing step a, start the system and operate the controller (15) to reset the device; c. Injection of digestive fluid: After performing step b, the digestive fluid injection pipe (22) of the Z-direction moving module (32) on the three-dimensional mechanical transmission device (2) holds and lifts the digestive fluid pipette (123) on the consumable storage device (3), turns on the digestive fluid pump (24) and the digestive fluid solenoid valve (23), and injects the digestive fluid in the digestive fluid reagent bottle (126) on the staining reagent input device (129) into the sputum cup (122) on the sputum homogenizer device (4) through the digestive fluid pipette (123); then the digestive fluid pipette (123) is thrown into the trash can (130); the cycle is repeated to complete the injection of liquid into each sputum cup (122) in step a; d. Sample homogenization step: After performing step c, start the oscillating stepper motor (45) on the sputum homogenizer (4) to drive the sputum cup (122) on the oscillating platform (44) to oscillate clockwise or counterclockwise for 5 to 10 minutes to obtain homogenized sputum; e. Sampling steps: After performing step d, the digestive fluid injection pipe (22) of the Z-direction moving module (32) on the three-dimensional mechanical transmission device (2) holds and lifts the liquefied sputum pipette (121) on the consumable storage device (3), turns on the suction pump (25) and the suction solenoid valve (26), and draws the homogenized sputum in the sputum cup (122) on the sputum homogenizing device (4) into the liquefied sputum pipette (121); at this time, the digestive fluid injection pipe (22) transfers the homogenized sputum to the centrifugal filter jacketed cup (120) on the consumable storage device (3); then the liquefied sputum pipette (121) is thrown into the trash can (130); the sampling of homogenized sputum in each sputum cup (122) in step d is completed by repeating the process. f. Liquid extraction step: After performing step e, turn on the liquid extraction solenoid valve (50) and liquid extraction pump (51) on the consumable storage device (3) to extract the excess homogenized sputum in the centrifugal filter jacketed cup (120). The liquid extraction time is 1 to 3 minutes. The bacteria in the homogenized sputum will adhere to the bottom of the centrifugal filter jacketed cup (120) to obtain a sputum film. g. Staining step: After performing step f, the dropper (133) of the two-dimensional mechanical transmission device (131) on the staining reagent input device (129) moves to the top of the centrifugal filter jacketed cup (120) on the consumable storage device (3), and the staining pump (60) and staining solenoid valve (64) are turned on, so that the staining liquid in a staining reagent bottle (63) drips into the sputum film of the centrifugal filter jacketed cup (120) and stands for 1 to 5 minutes to complete the staining. At this time, the staining film is obtained from the bottom of the centrifugal filter jacketed cup (120). h. Repeat steps f and g to stain the film with the staining solution in 3 to 5 staining reagent bottles (63) in turn to obtain multiple staining films; i. Step of capturing dyeing images: After performing step g, the contour camera drive module (33) of the Z-direction moving module (32) on the three-dimensional mechanical transmission device (2) moves to the top of the centrifugal filter jacketed cup (120) on the consumable storage device (3), captures images of the dyed film multiple times, and uploads them to the computer display screen (117) for analysis. j. Heating step: After completing step i, the electric gripper (37) in the Z-direction moving module (32) on the three-dimensional mechanical transmission device (2) picks up the centrifugal filter jacketed cup (120) on the consumable storage device (3); and moves it above the heater (124) to heat the multi-dyed film for 5 to 10 minutes to obtain the dried dyed film; k. Oil dripping step: After completing step j, the slide moving support (84) and the slide lifting rod (92) in the slide preparation device (78) first move the slide (85) in the slide storage box (82) to the oil dripping position on the slide operation platform (83), open the oil suction solenoid valve (65) on the slide operation platform (83), inject the microscope oil in the oil bottle (102) into the oil injection piston (97), and then open the oil injection solenoid valve (98) on the oil dripping device (90) to drip the microscope oil in the oil injection piston (97) onto the slide (85) through the oil dripping needle (103) on the slide operation platform (83) to obtain the oil-dried slide (85). m. Slide preparation steps: After completing step k, the slide moving bracket (84) in the slide preparation machine (78) moves the oil-spraying slide (85) to the slide preparation position on the slide operation platform (83). The electric gripper (37) of the Z-direction moving module (32) on the three-dimensional mechanical transmission device (2) places the dried staining film at the bottom of the centrifugal filter jacketed cup (120) onto the oil-spraying slide (85). Then, the coverslip pump (116) and coverslip suction valve (115) are opened. The vacuum suction cup (114) on the suction cup connection platform (112) picks up the coverslip (106) in the coverslip storage box (105) and presses it onto the dried staining film in the centrifugal filter jacketed cup (120), so that it remains between the oil-spraying slide (85) and the coverslip (106), thus completing the preparation of a sputum smear slide. n. Slide feeding step: After completing step m, the electric gripper (37) of the Z-direction moving module (32) on the three-dimensional mechanical transmission device (2) throws the used centrifugal filter jacketed cup (120) into the trash can (130), and moves the slide operation platform (83), slide moving bracket (84) in the slide preparation device (78) and the slide collection rack (70) in the slide collection device (73) to send the sputum smear slide into the slide collection rack (70); p. Repeat steps j, k, m and n to sequentially prepare sputum smear slides from the multiple staining films at the bottom of each centrifugal filter jacketed cup (120). Finally, remove the slide collection rack (70) from the system to obtain a batch of sputum smear slides.

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