Temperature control device and method for microfluidic chip

Abstract

This invention discloses a temperature control device and method for microfluidic chips, comprising: arranging a microfluidic chip to be temperature-controlled at a first position on a temperature control plate; arranging an equivalent temperature sensing chip at a second position adjacent to the first position on the temperature control plate; embedding a temperature sensor in the equivalent temperature sensing chip and monitoring the temperature of the equivalent temperature sensing chip through the temperature sensor; estimating the temperature of the microfluidic chip to be temperature-controlled based on the monitored temperature of the equivalent temperature sensing chip; and adjusting the output temperature of the temperature control plate according to the estimated temperature of the microfluidic chip to be temperature-controlled and a target temperature. This invention indirectly monitors the temperature of the chip to be temperature-controlled by configuring an equivalent temperature sensing chip, and adjusts the output temperature of the temperature control plate according to the monitored temperature of the equivalent temperature sensing chip and the target temperature. This allows for accurate monitoring of the temperature of the chip to be temperature-controlled without embedding a sensor in the chip, thereby enabling precise temperature control of the chip through the temperature control plate.

Description

Technical Field

[0001] This invention relates to the field of microfluidic temperature control technology, and in particular to a temperature control device and method for microfluidic chips. Background Technology

[0002] The ultimate goal of Lab-on-a-Chip (LOC) research is to connect and coexist multiple units or modules with different functions at the microscale, enabling them to collaboratively complete a series of complex biochemical analyses, such as sample preparation, biological and chemical reactions, and separation and detection. Ultimately, all functional modules involved in fields such as biology and chemistry can be integrated onto a chip of just a few square centimeters, directly applicable to biochemical detection, rapid environmental detection, and more. However, existing LOCs that have been gradually deployed primarily rely on pressure-driven and thermal-driven methods for their core function—microfluidic actuation. These methods require external power, involve relatively large volumes of fluid, have numerous flow channel components, and consume high power. Furthermore, the actuation methods lack versatility across different devices, failing to fully utilize the effective function of the "droplet," the basic microfluidic manipulator. Therefore, developing an effective and easy-to-operate microfluidic platform-level droplet manipulation method is crucial for the future development of LOCs.

[0003] Electrowetting-on-dielectric (EWOD) involves adding a thin insulating film between a metal electrode and the electrolyte. When a certain voltage is applied between the liquid and the electrode, the surface tension of the liquid and solid undergoes a reversible change, manifested as a change in the contact angle of the droplet on the solid surface. When the contact angle changes symmetrically and uniformly, the droplet macroscopically exhibits a process of spreading from a spherical droplet into a liquid film. However, if the contact angle changes asymmetrically, a surface tension gradient appears at the contact lines on both sides of the droplet, leading to droplet migration and movement. This is the theoretical basis for droplet manipulation in on-chip lab applications.

[0004] As the principle suggests, by utilizing the electrowetting effect and manipulating the voltage of electrodes, tiny droplets can be controlled on a chip. Specific manipulations include migration, segmentation, mixing, and oscillation. Through the combination of these functions, various biological and chemical experimental procedures can be transferred to the chip, thus realizing a laboratory-on-a-chip (PAC) system. Biological and chemical experimental procedures are quite complex, each requiring numerous electrodes. Therefore, one of the key technologies for realizing a PAC chip is the formation of a large number of driving electrodes and the provision of driving signals to drive the droplets to operate as required.

[0005] By combining the principle of electrowetting with arrayed driving electrode plates, a digital microfluidic chip with a certain degree of droplet manipulation capability is formed. Functional droplets replace ordinary droplets, and specific reagent handling processes are mapped onto the digital microfluidic chip, resulting in a digital microfluidic chip with certain reagent processing capabilities. In some biological or chemical applications, the internal liquid temperature of the microfluidic chip needs precise control to ensure the normal progress of biochemical reactions, especially in the formation of molecular-level products, where both yield and output are affected by the reaction temperature.

[0006] Existing temperature control methods are relatively mature, but efficient and accurate temperature measurement methods are lacking. Common temperature measurement methods include, on the one hand, using a bottom-mounted temperature control plate for temperature feedback. This fixed method avoids the need to replace sensors during chip replacement, saving on sensor and calibration costs. However, since chips are mostly exposed to room temperature, the temperature control boundary conditions are unstable, leading to errors and conduction delays between the temperature fed back from the temperature control plate and the internal chip temperature, potentially reducing the stability of internal chip temperature control. On the other hand, various methods embed sensors inside the chip. This method allows for in-situ measurement of temperature changes within the microfluidic chip, eliminating fluctuation and delay issues. The drawback is that the integrated design of the temperature sensor and chip increases chip and sensor calibration costs, while also reducing the usable space of the microfluidic channels. Summary of the Invention

[0007] In view of the problems existing in the prior art, the purpose of the present invention is to provide a temperature control method, device, electronic device and computer-readable medium for microfluidic chips, thereby overcoming at least to some extent one or more problems caused by the limitations and defects of the prior art.

[0008] To achieve the above objectives, the first aspect of the present invention provides a temperature control method for a microfluidic chip, comprising the following steps:

[0009] The microfluidic chip to be temperature-controlled is placed in the first position on the temperature control board;

[0010] An equivalent temperature sensing chip is arranged at a second position adjacent to the first position on the temperature control plate;

[0011] A temperature sensor is embedded in the equivalent temperature sensing chip, and the temperature of the equivalent temperature sensing chip is monitored by the temperature sensor.

[0012] The temperature of the microfluidic chip to be controlled is estimated based on the monitored equivalent temperature chip temperature.

[0013] The output temperature of the temperature control board is adjusted based on the estimated temperature of the microfluidic chip to be controlled and the target temperature.

[0014] Furthermore, the equivalent temperature sensing chip has the same structure as the microfluidic chip and is located in the same temperature environment.

[0015] Furthermore, a microfluidic chip to be temperature-controlled is arranged symmetrically at a first position along the central axis of the temperature control plate, and an equivalent temperature measuring chip is arranged at a second position.

[0016] Furthermore, it also includes:

[0017] The temperature in the equivalent temperature sensing chip is monitored in real time by the temperature sensor.

[0018] The monitored equivalent temperature of the temperature sensing chip and the target temperature of the microfluidic chip are sent to the control unit.

[0019] The control unit estimates the temperature of the monitored equivalent temperature sensor chip as the temperature of the microfluidic chip.

[0020] A temperature control signal is generated based on the estimated temperature of the microfluidic chip and the target temperature control temperature of the microfluidic chip and sent to the temperature control board;

[0021] The control board adjusts the output temperature of the temperature control board according to the temperature control signal.

[0022] Furthermore, it also includes:

[0023] Compare the equivalent temperature sensing chip temperature with the target temperature control temperature of the microfluidic chip;

[0024] When the equivalent temperature sensor chip temperature and the target temperature control temperature are the same, the temperature control board stops working; otherwise, the temperature control board generates a temperature control signal based on the equivalent temperature sensor chip temperature and the target temperature control temperature to adjust the temperature of the temperature control board.

[0025] A second aspect of the present invention provides a temperature control device for microfluidic chips, comprising a control unit, a temperature control board, and an equivalent temperature sensing chip;

[0026] The temperature control board is equipped with a microfluidic chip to be controlled and an equivalent temperature measuring chip, which are used to regulate the temperature of the microfluidic chip to be controlled and the equivalent temperature measuring chip.

[0027] A temperature sensor is embedded in the equivalent temperature sensing chip, and the temperature of the equivalent temperature sensing chip is monitored by the temperature sensor.

[0028] The control unit estimates the temperature of the microfluidic chip to be controlled based on the monitored equivalent temperature sensing chip temperature, and adjusts the output temperature of the temperature control board based on the estimated temperature of the microfluidic chip to be controlled and the target temperature of the microfluidic chip.

[0029] Furthermore, the microfluidic chip is provided with a temperature sensor lead that is completely mirrored with the equivalent temperature sensing chip, and the microfluidic chip at the mirror position of the temperature sensor of the equivalent temperature sensing chip is either not provided with a sensor or is provided with a pseudo-sensor with the same structure as the temperature sensor.

[0030] Furthermore, the equivalent temperature sensing chip is driven synchronously with the microfluidic chip, and the position and driving path of the droplets in the equivalent temperature sensing chip are completely mirror images of the position and path of the droplets in the microfluidic chip.

[0031] A third aspect of the present invention provides an electronic device, comprising:

[0032] One or more processors; and

[0033] A storage device for storing one or more programs, which, when executed by one or more processors, cause the one or more processors to perform the methods described above.

[0034] A fourth aspect of the present invention provides a computer-readable medium having a computer program stored thereon, characterized in that the program, when executed by a processor, implements the above-described method.

[0035] This invention indirectly monitors the temperature of the chip to be controlled by configuring an equivalent temperature sensing chip, and adjusts the output temperature of the temperature control board according to the monitored equivalent temperature sensing chip temperature and the target temperature. Without implanting a sensor into the chip to be controlled, the temperature of the chip to be controlled can be accurately monitored, and then the temperature control board can accurately control the temperature of the chip to be controlled. Attached Figure Description

[0036] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0037] Figure 1 This is a schematic diagram of a temperature control device for a microfluidic chip according to an embodiment of the present invention;

[0038] Figure 2 This is a flowchart of a temperature control method for a microfluidic chip according to an embodiment of the present invention. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0040] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0041] like Figure 1 As shown, a temperature control device for a microfluidic chip according to an embodiment of the present invention includes a control unit (not shown in the figure), a temperature control plate 11 and an equivalent temperature measuring chip 12.

[0042] The temperature control plate 11 is provided with a microfluidic chip 13 to be controlled and an equivalent temperature measuring chip 12, which are used to regulate the temperature of the microfluidic chip 13 to be controlled and the equivalent temperature measuring chip 12.

[0043] A temperature sensor 14 is embedded in the equivalent temperature sensing chip 12, and the temperature of the equivalent temperature sensing chip 12 is monitored by the temperature sensor 14. The temperature sensor can be embedded in the center of the equivalent temperature sensing chip to improve the accuracy of monitoring the temperature of the equivalent temperature sensing chip.

[0044] The control unit estimates the temperature of the microfluidic chip 13 to be controlled based on the monitored temperature of the equivalent temperature measuring chip 12, and adjusts the output temperature of the temperature control board 11 according to the estimated temperature of the microfluidic chip 13 to be controlled and the target temperature of the microfluidic chip 13.

[0045] In this embodiment, the equivalent temperature control chip 12 can be directly integrated onto the temperature control board, and the microfluidic chip 13 to be measured is positioned on the temperature control board 11 adjacent to the equivalent temperature sensing chip 12. This ensures, to a certain extent, that the temperature environments of the microfluidic chip 13 and the equivalent temperature sensing chip 12 are essentially the same, reducing the interference of different ambient temperatures on the temperature measurement results. The equivalent temperature sensing chip and the microfluidic chip to be controlled are preferably arranged symmetrically along the central axis of the temperature control board at a first position and a second position.

[0046] In this embodiment, the equivalent temperature sensing chip 12 and the microfluidic chip 13 have the same structure, both including a base plate and a cover plate. The cover plate seals the base plate and forms a channel for droplet flow between the cover plate and the base plate. The base plate includes a substrate electrode layer, a dielectric layer, and a hydrophobic layer, which are stacked sequentially. A driving electrode is formed in the electrode layer, which can control the position of the droplet in the flow channel under the action of the driving electrode, realizing operations such as migration, segmentation, mixing, and oscillation. The equivalent temperature sensing chip is completely modeled after the microfluidic chip, which can ensure that the temperature characteristics of the microfluidic chip 13 and the equivalent temperature sensing chip 12 are basically consistent to a certain extent, reducing interference with the temperature measurement results.

[0047] In one embodiment of the present invention, in order to further improve the accuracy of temperature measurement, the equivalent temperature measuring chip is also driven in the same way as the microfluidic chip, and the position and driving path of the droplets in the equivalent temperature measuring chip are completely mirrored with the position and driving path of the droplets in the microfluidic chip, thereby reducing the influence of the movement of the droplets on the temperature distribution during the driving process, so that the equivalent temperature measuring chip can more accurately reflect the true temperature of the microfluidic chip.

[0048] In one embodiment of the present invention, in order to further improve the accuracy of temperature measurement, a temperature sensor lead wire that is completely mirrored with the equivalent temperature measurement chip is provided in the microfluidic chip (no temperature sensor is provided), thereby reducing the influence of the temperature sensor lead wire itself on heat transfer and temperature distribution.

[0049] In one embodiment of the present invention, in order to further improve the accuracy of temperature measurement, a temperature sensor lead wire that is completely mirrored with an equivalent temperature measurement chip is provided in the microfluidic chip (the structure is similar to that of a temperature sensor, but it does not actually measure temperature), thereby reducing the influence of the temperature sensor lead wire itself on heat transfer and temperature distribution.

[0050] like Figure 2 As shown, a temperature control method for a microfluidic chip according to an embodiment of the present invention includes the following steps:

[0051] Step S210: Arrange the microfluidic chip to be temperature controlled at the first position on the temperature control plate;

[0052] Step S220: Arrange an equivalent temperature sensing chip at a second position adjacent to the first position on the temperature control plate;

[0053] Step S230: Implant a temperature sensor in the equivalent temperature sensing chip, and monitor the temperature of the equivalent temperature sensing chip through the temperature sensor;

[0054] Step S240: Estimate the temperature of the microfluidic chip to be controlled based on the monitored equivalent temperature of the temperature sensing chip;

[0055] Step S250: Adjust the output temperature of the temperature control board according to the estimated temperature of the microfluidic chip to be controlled and the target temperature.

[0056] In one embodiment of the present invention, the temperature control method further includes:

[0057] The temperature in the equivalent temperature sensing chip is monitored in real time by the temperature sensor.

[0058] The monitored equivalent temperature of the temperature sensing chip and the target temperature of the microfluidic chip are sent to the control unit.

[0059] The control unit estimates the temperature of the equivalent temperature sensing chip as the temperature of the microfluidic chip by monitoring the equivalent temperature sensing chip; the control unit directly regards the temperature of the equivalent temperature sensing chip as the temperature of the microfluidic chip.

[0060] A temperature control signal is generated based on the estimated temperature of the microfluidic chip and the target temperature control temperature of the microfluidic chip and sent to the temperature control board;

[0061] The control board adjusts the output temperature of the temperature control board according to the temperature control signal.

[0062] In one embodiment of the present invention, the temperature control method further includes:

[0063] Compare the equivalent temperature sensing chip temperature with the target temperature control temperature of the microfluidic chip;

[0064] When the equivalent temperature sensing chip temperature and the target temperature control temperature are the same, the temperature control board stops working; otherwise, the temperature control board generates a temperature control signal based on the equivalent temperature sensing chip temperature and the target temperature control temperature to adjust its temperature. For example, when the equivalent temperature sensing chip temperature and the target temperature control temperature are the same, it indicates that the temperature of the microfluidic chip to be controlled has been regulated. At this time, the control unit can send a stop signal to the temperature control board to stop the temperature control of the microfluidic chip. When the equivalent temperature sensing chip temperature is lower than the target temperature control temperature, it indicates that the temperature of the microfluidic chip needs to be increased. At this time, the control unit can send a heating signal to the temperature control board to increase the output temperature of the microfluidic chip to be controlled. When the equivalent temperature sensing chip temperature is higher than the target temperature control temperature, it indicates that the temperature of the microfluidic chip needs to be decreased. At this time, the control unit can send a cooling signal to the temperature control board to decrease the output temperature of the microfluidic chip to be controlled.

[0065] In summary, this invention addresses the challenges of temperature measurement and inaccurate temperature control in the droplet flow space of digital microfluidic chips and other microfluidic chips during practical applications, due to limited space and poor environmental sealing. By placing a mirror device on the temperature control board, the temperature changes of the original device can be monitored equivalently in a substitute manner. This solution provides an effective temperature measurement method for digital microfluidic chip applications, while avoiding the process complexity of implanted or attached temperature measurement methods, thus reducing mass production costs.

[0066] Furthermore, in an exemplary embodiment of this disclosure, an electronic device capable of implementing the above-described method is also provided.

[0067] Those skilled in the art will understand that various aspects of the present invention can be implemented as systems, methods, or program products. Therefore, various aspects of the present invention can be specifically implemented as entirely hardware embodiments, entirely software embodiments (including firmware, microcode, etc.), or embodiments combining hardware and software aspects, collectively referred to herein as “circuit,” “module,” or “system.”

[0068] An electronic device according to an embodiment of the present invention is described below. The electronic device is manifested in the form of a general-purpose computing device. The components of the electronic device may include, but are not limited to: at least one processing unit described above, at least one storage unit described above, a bus connecting different system components (including the storage unit and the processing unit), and a display unit.

[0069] The storage unit stores program code that can be executed by the processing unit, causing the processing unit to perform the steps described in the "Exemplary Methods" section above, based on various exemplary embodiments of the present invention. For example, the processing unit can perform actions such as... Figure 2 Steps S210 to S250 are shown in the figure.

[0070] The storage unit may include readable media in the form of volatile storage units, such as random access memory (RAM) and / or cache storage units, and may further include read-only memory (ROM).

[0071] The storage unit may also include a program / utility having a set (at least one) of program modules, including but not limited to: an operating system, one or more application programs, other program modules, and program data, each or some combination of these examples may include an implementation of a network environment.

[0072] A bus can represent one or more of several bus architectures, including memory units.

[0073] A bus or memory unit controller, peripheral bus, graphics acceleration port, processing unit, or local bus using any of the various bus architectures.

[0074] The electronic device can also communicate with one or more external devices (e.g., keyboards, pointing devices, Bluetooth devices, etc.), one or more devices that enable a user to interact with the electronic device, and / or any device that enables the electronic device to communicate with one or more other computing devices (e.g., routers, modems, etc.). This communication can be achieved through input / output (I / O) interfaces. Furthermore, the electronic device can communicate with one or more networks (e.g., local area networks (LANs), wide area networks (WANs), and / or public networks, such as the Internet) via a network adapter. As shown in the figure, the network adapter communicates with other modules of the electronic device via a bus. It should be understood that, although not shown in the figure, other hardware and / or software modules can be used in conjunction with the electronic device, including but not limited to: microcode, device drivers, redundant processing units, external disk drive arrays, RAID systems, tape drives, and data backup storage systems.

[0075] From the above description of the embodiments, those skilled in the art will readily understand that the exemplary embodiments described herein can be implemented by software or by combining software with necessary hardware. Therefore, the technical solutions according to the embodiments of this disclosure can be embodied in the form of a software product, which can be stored in a non-volatile storage medium (such as a CD-ROM, USB flash drive, external hard drive, etc.) or on a network, including several instructions to cause a computing device (such as a personal computer, server, terminal device, or network device, etc.) to execute the methods according to the embodiments of this disclosure.

[0076] In exemplary embodiments of this disclosure, a computer-readable storage medium is also provided, on which a program product capable of implementing the methods described above is stored. In some possible embodiments, various aspects of the invention may also be implemented as a program product comprising program code that, when the program product is run on a terminal device, causes the terminal device to perform the steps of the various exemplary embodiments of the invention described in the "Exemplary Methods" section above.

[0077] A program product for implementing the above-described method according to embodiments of the present invention is described. This product may employ a portable compact disc read-only memory (CD-ROM) and include program code, and may run on a terminal device, such as a personal computer. However, the program product of the present invention is not limited thereto. In this document, the readable storage medium may be any tangible medium containing or storing a program that may be used by or in conjunction with an instruction execution system, apparatus, or device.

[0078] The program product may employ any combination of one or more readable media. A readable medium may be a readable signal medium or a readable storage medium. A readable storage medium may be, for example, but not limited to, an electrical, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination thereof. More specific examples of readable storage media (a non-exhaustive list) include: an electrical connection having one or more wires, a portable disk, a hard disk, random access memory (RAM), read-only memory (ROM), erasable programmable read-only memory (EPROM or flash memory), optical fiber, portable compact disk read-only memory (CD-ROM), optical storage devices, magnetic storage devices, or any suitable combination thereof.

[0079] Computer-readable signal media may include data signals propagated in baseband or as part of a carrier wave, carrying readable program code. Such propagated data signals may take various forms, including but not limited to electromagnetic signals, optical signals, or any suitable combination thereof. A readable signal medium may also be any readable medium other than a readable storage medium, capable of sending, propagating, or transmitting programs for use by or in conjunction with an instruction execution system, apparatus, or device.

[0080] The program code contained on the readable medium may be transmitted using any suitable medium, including but not limited to wireless, wired, optical fiber, RF, etc., or any suitable combination thereof.

[0081] Program code for performing the operations of this invention can be written in any combination of one or more programming languages, including object-oriented programming languages ​​such as Java and C++, and conventional procedural programming languages ​​such as C or similar languages. The program code can execute entirely on the user's computing device, partially on the user's device, as a standalone software package, partially on the user's computing device and partially on a remote computing device, or entirely on a remote computing device or server. In cases involving remote computing devices, the remote computing device can be connected to the user's computing device via any type of network, including a local area network (LAN) or a wide area network (WAN), or it can be connected to an external computing device (e.g., via the Internet using an Internet service provider).

[0082] Furthermore, the above figures are merely illustrative of the processes included in the method according to exemplary embodiments of the present invention, and are not intended to be limiting. It is readily understood that the processes shown in the above figures do not indicate or limit the temporal order of these processes. Additionally, it is readily understood that these processes may be executed synchronously or asynchronously, for example, in multiple modules.

[0083] Other embodiments of this disclosure will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of this disclosure that follow the general principles of this disclosure and include common knowledge or customary techniques in the art not disclosed herein. The specification and embodiments are to be considered exemplary only, and the true scope and spirit of this disclosure are indicated by the claims.

[0084] It should be understood that this disclosure is not limited to the precise structures described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from its scope. The scope of this disclosure is limited only by the appended claims.

Claims

1. A temperature control method for microfluidic chips, characterized in that, Includes the following steps: A microfluidic chip to be temperature controlled is arranged at a first position symmetrically along the central axis of the temperature control plate; An equivalent temperature sensing chip is arranged symmetrically along the central axis of the temperature control plate and at a second position adjacent to the first position; the equivalent temperature sensing chip has the same structure as the microfluidic chip and is in the same temperature environment; A temperature sensor is embedded in the equivalent temperature sensing chip, and the temperature of the equivalent temperature sensing chip is monitored by the temperature sensor. The temperature of the microfluidic chip to be controlled is estimated based on the monitored equivalent temperature chip temperature. The output temperature of the temperature control board is adjusted based on the estimated temperature of the microfluidic chip to be controlled and the target temperature.

2. The temperature control method as described in claim 1, characterized in that, Also includes: The temperature in the equivalent temperature sensing chip is monitored in real time by the temperature sensor. The monitored equivalent temperature of the temperature sensing chip and the target temperature of the microfluidic chip are sent to the control unit. The control unit estimates the temperature of the monitored equivalent temperature sensor chip as the temperature of the microfluidic chip. A temperature control signal is generated based on the estimated temperature of the microfluidic chip and the target temperature control temperature of the microfluidic chip and sent to the temperature control board; The control unit adjusts the output temperature of the temperature control plate according to the temperature control signal.

3. The temperature control method as described in claim 2, characterized in that, Also includes: Compare the equivalent temperature sensing chip temperature with the target temperature control temperature of the microfluidic chip; When the equivalent temperature sensor chip temperature and the target temperature control temperature are the same, the temperature control board stops working; otherwise, the temperature control board generates a temperature control signal based on the equivalent temperature sensor chip temperature and the target temperature control temperature to adjust the temperature of the temperature control board.

4. A temperature control device for microfluidic chips, characterized in that, Includes a control unit, a temperature control board, and an equivalent temperature sensing chip; The temperature control board is provided with a microfluidic chip to be controlled and an equivalent temperature measuring chip, which are used to regulate the temperature of the microfluidic chip to be controlled and the equivalent temperature measuring chip; the microfluidic chip and the equivalent temperature measuring chip are arranged symmetrically along the central axis of the temperature control board, and the equivalent temperature measuring chip has the same structure as the microfluidic chip and is in the same temperature environment; A temperature sensor is embedded in the equivalent temperature sensing chip, and the temperature of the equivalent temperature sensing chip is monitored by the temperature sensor. The control unit estimates the temperature of the microfluidic chip to be controlled based on the monitored equivalent temperature sensing chip temperature, and adjusts the output temperature of the temperature control board based on the estimated temperature of the microfluidic chip to be controlled and the target temperature of the microfluidic chip.

5. The temperature control device as described in claim 4, characterized in that, The microfluidic chip has a temperature sensor lead that is completely mirrored in the equivalent temperature sensing chip, and the microfluidic chip does not have a sensor or has a pseudo-sensor with the same structure as the temperature sensor in the mirror position of the equivalent temperature sensing chip.

6. The temperature control device as described in claim 4, characterized in that, The equivalent temperature sensing chip is driven synchronously with the microfluidic chip, and the position and driving path of the droplets in the equivalent temperature sensing chip are completely mirror images of the position and path of the droplets in the microfluidic chip.

7. An electronic device, characterized in that, include: One or more processors; as well as A storage device for storing one or more programs, which, when executed by the one or more processors, cause the one or more processors to implement the method according to any one of claims 1 to 3.

8. A computer-readable medium having a computer program stored thereon, characterized in that, When the program is executed by the processor, it implements the method according to any one of claims 1 to 3.

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