Water-soluble anion-modified square-planar platinum (II) complex electrochemiluminescence material, preparation method and application thereof

Abstract

The application discloses a water-soluble anion-modified square plate type platinum (II) complex electrochemiluminescence material and a preparation method and application thereof. 2,6-bis(benzimidazole) pyridine is reacted with 1,3-propanesulfonic acid lactone in a DMSO solution to obtain a ligand L1, and then the ligand L1 is reacted with potassium chloroplatinite under a nitrogen atmosphere to obtain a platinum complex. The square plate type platinum (II) complex has been verified to have excellent photophysical properties, and is introduced into the electrochemiluminescence field for the first time. By comparing the electrochemiluminescence performance of a commercial Ru(bpy)3 2+ system under the same concentration, the cathode electrochemiluminescence of the material is about 35 times stronger than that of the Ru(bpy)3 2+ system under the dispersed state test condition; the anode electrochemiluminescence of the material is about 10 times stronger than that of the Ru(bpy)3 2+ system under the non-homogeneous aggregation state test condition; the cathode electrochemiluminescence of the material is about 16 times stronger than that of the Ru(bpy)3 2+ system.

Description

Technical Field

[0001] This invention belongs to the field of electrochemiluminescence technology, specifically relating to a water-soluble anion-modified prismatic platinum(II) complex electrochemiluminescent material, its preparation method, and its application. Background Technology

[0002] Due to its unique reaction mechanism, electrochemiluminescence possesses advantages such as high sensitivity and low background noise, making it one of the most important analytical methods in in vitro diagnostics and analytical detection. Almost all current applications are based on luminescently labeled Ru(bpy)3. 2+ The anodic electrochemiluminescence of the -TPrA system is limited by the ACQ effect in the signal polymerization amplification process of traditional electrochemiluminescent materials. Therefore, there is an urgent need to develop an electrochemiluminescent material with better performance than the traditional system that can be used for biolabeling processes.

[0003] Complexes of platinum(II) d8 tridentate nitrogen ligands have attracted considerable attention due to their rich spectra and excellent luminescence properties. The strong spin-orbit coupling (SOC) imposed by heavy atoms leads to a low-energy triplet excited state configuration via intersystem crossing (ISC), resulting in efficient radiative decay processes towards the singlet ground state. Photoexcitation of these compounds in the UV-VIS region leads to the formation of excited states based on their electronic transition configurations, such as metal-center (MC), ligand-center (LC), interligand or ligand-ligand charge transfer (ILCT or LLCT), ligand-metal charge transfer (LMCT), and metal-ligand charge transfer (MLCT). Due to the tendency for high stacking caused by the square planar geometry, weak non-covalent metal-metal and ligand-ligand interactions are established between ground-state molecules via the π-electron cloud of the aromatic ring, forming new excited states, such as metal-metal-ligand charge transfer, i.e., MMLCT (dσ*→π*) and ligand-metal-metal charge transfer (LMMCT). These charge-transfer (CT) states exhibit absorption and luminescence properties that show a greater size-scale color shift, higher emission quantum yield, and longer excited-state lifetime compared to the light of self-non-interacting platinum complexes. Systems based on this self-assembled functional structure have been reported for use in optoelectronic devices and as labeled molecules in cancer diagnosis and treatment. However, due to the extremely poor solubility of these substances, most photophysical properties are studied in solid form. While a few reports have been made on the electrochemiluminescence properties of platinum(II) complexes, proposing the concept of AIECL (Aggregation-Induced Electrochemiluminescence of Platinum(II) Complexes, such as Serena et al.'s first report on the electrochemiluminescence of platinum complexes in aqueous solution, describing the synthesis, solution, and solid-state photophysical properties of two square planar Pt(II) complexes, and proposing the concept of AIECL (Journal of the American Chemical Society, 2017), the electrochemiluminescence efficiency of platinum(II) complexes themselves and the difficulty in biolabeling modifications limit their further application in the field of electrochemiluminescence. Therefore, how to functionalize these substances, improve their solubility, enhance their electrochemiluminescence efficiency, and propose useful biomarker strategies are of great significance for their practical commercial application.

[0004] Based on the above background, this invention discovers and synthesizes a platinum complex modified with sulfonate ions, which exhibits excellent AIECL performance, providing a new approach for developing electrochemiluminescent reagents with AIECL performance. Summary of the Invention

[0005] To address the problems existing in the prior art, the present invention aims to provide a water-soluble amphiphilic anion-modified lattice-type platinum(II) complex electrochemiluminescent material, its preparation method, and its application.

[0006] This invention introduces amphiphilic lattice-type platinum(II) complexes into the field of electrochemiluminescence for the first time, and tests their electrochemiluminescence performance. It was found that the amphiphilic lattice-type platinum(II) complex is an excellent electrochemiluminescence reagent. Furthermore, due to the strong coordination ability of platinum, functional groups can be modified on platinum atoms to make them functionalized for use in biolabeling processes.

[0007] Compared with commercial Ru(bpy)3 at the same concentration 2+ Regarding the electrochemiluminescence performance of the system, the inventors discovered that under dispersed testing conditions, the cathodic electrochemiluminescence intensity of the latticeal platinum(II) complex electrochemiluminescent material of this invention is approximately 35 times stronger; under heterogeneous aggregated testing conditions, the anodic electrochemiluminescence intensity of the latticeal platinum(II) complex electrochemiluminescent material of this invention is approximately 10 times stronger, and the cathodic electrochemiluminescence intensity is approximately 16 times stronger. Therefore, it can be concluded that the performance of this type of luminescent reagent far surpasses that of commercial systems and fills the gap in cathodic electrochemiluminescence among commercial reagents. This application helps to solve the ACQ effect existing in the polymerization amplification process of traditional luminescent materials, exhibits excellent electrochemiluminescence performance, promotes the development of cathodic electrochemiluminescence processes, lays the foundation for the updating and iteration of commercial luminescent reagents, and its excellent AIECL performance also broadens the ideas for the design and practical application of signal polymerization amplification strategies.

[0008] The objective of this invention is achieved through the following technical solutions.

[0009] This invention provides a method for preparing a water-soluble, anion-modified, lattice-type platinum(II) complex electrochemiluminescent material. The main experimental raw materials include 2,6-bis(benzimidazole)pyridine, potassium chloroplatinate, tetrabutylammonium bromide, and 1,3-propanesulfonic acid lactone. The preparation method specifically includes the following steps:

[0010] (1) Prepare a 2,6-bis(benzimidazole)pyridine solution of a certain concentration: Dissolve a certain amount of 2,6-bis(benzimidazole)pyridine in DMSO solution, slowly add potassium hydroxide aqueous solution, and stir until uniform;

[0011] (2) Add tetra-n-butylammonium bromide solid to the solution prepared in step (1);

[0012] (3) Purge the solution from step (2) with nitrogen to remove oxygen, and add DMSO solution of 1,3-propanesulfonic acid lactone dropwise to carry out the reaction;

[0013] (4) After the reaction in step (3) is completed, the water is removed by depressurization and acetone is added to precipitate the crude product L1.

[0014] (5) Dissolve the crude product obtained in step (4) in DMSO solution, then add potassium chloroplatinate, purge with nitrogen, heat under reflux to obtain a yellow suspension;

[0015] (6) Add methanol and acetone to the suspension obtained in step (5) to completely precipitate the product, and centrifuge to obtain the solid final product 1K.

[0016] Furthermore, the concentration of the 2,6-bis(benzimidazole)pyridine solution prepared in step (1) is 0.5-0.7 mol / L.

[0017] Furthermore, the concentration of the potassium hydroxide aqueous solution used in step (1) is 2.0-3.0 mol / L.

[0018] Furthermore, in step (2), the amount of tetra-n-butylammonium bromide solid added is 0.3-0.5 mmol.

[0019] Furthermore, the nitrogen purging time in step (3) is set to 5-10 min.

[0020] Further, the concentration of the DMSO solution of 1,3-propanesulfonic acid lactone in step (3) is 2.0-3.0 mol / L.

[0021] Furthermore, in step (3), the temperature is controlled at 120-180℃ and the reaction time is 10-18h.

[0022] Further, after adding acetone to precipitate in step (4), wash three times with acetone.

[0023] Furthermore, the concentration of the crude product dissolved in DMSO solution in step (5) is 0.2-0.5 mol / L.

[0024] Further, in step (5), the amount of potassium chloroplatinate added is 1.0-3.0 mmol.

[0025] Furthermore, in step (5), the reflux temperature is 120-180℃, and the reflux time is 10-18 hours.

[0026] Further, after precipitation in step (6), the product is washed three times with methanol and acetone in sequence.

[0027] The prismatic platinum(II) complex electrochemiluminescent material provided by this invention contains platinum. Based on the coordination effect of platinum, it can be further modified with labeling groups for use in biolabeling processes. Figure 2The structural formula of the prismatic platinum(II) complex prepared in this invention, along with several groups that can replace chlorine atoms, are used for biolabeling. The derivatized ligands are bound to the complex through coordination between platinum metal and pyridine and alkynyl groups. This product can be used not only in traditional biolabeling strategies but also without affecting its electrochemiluminescence performance. It mainly includes three ligand derivatization forms: carboxyl-NSH-activated ester-derived ligands can react with amino groups in conventional antibodies or RNA to form amide bonds, thereby achieving biolabeling; biotin-structured derivatized ligands can specifically bind to streptavidin-modified antibodies, thereby achieving biolabeling; and boric acid-structured derivatized ligands can specifically recognize cell surface glycoproteins, thereby achieving cell labeling. These derivatized ligands can label the prismatic platinum(II) complex onto target molecules through simple reactions, thus applying it to electrochemiluminescence immunosensing systems. This process simplifies the modification of luminescent molecules, and its excellent electrochemiluminescence performance can effectively improve the detection sensitivity of traditional electrochemiluminescence immunosensing systems.

[0028] Compared with the prior art, the advantages of the technical solution of the present invention are as follows:

[0029] (1) Complexes of platinum(II) d8 tridentate nitrogen ligands have attracted much attention due to their rich spectra and excellent luminescence properties. However, due to their extremely poor solubility, most photophysical properties are studied based on solids. The platinum(II) complex synthesized in this invention has the advantage of water solubility because the sulfonate anion is a water-soluble group. By modifying the sulfonate anion onto the cyclic ligand of the platinum complex, the water solubility of the platinum complex is enhanced.

[0030] (2) The square-shaped platinum(II) complex electrochemiluminescent material prepared in this invention is compared with commercial Ru(bpy)3 at the same concentration. 2+ The system exhibits superior electrochemiluminescence performance. Specifically, under dispersed testing conditions, its cathodic electrochemiluminescence is superior to that of Ru(bpy)3. 2+ The system is approximately 35 times stronger. Under test conditions of heterogeneous aggregation, the anodic electrochemiluminescence of this material is greater than that of Ru(bpy)3. 2+ The system is about 10 times stronger, and the cathodic electrochemiluminescence is better than Ru(bpy)3. 2+ The system is about 16 times stronger.

[0031] (3) The square-shaped platinum(II) complex electrochemiluminescent material prepared by the present invention can be further modified with labeling groups based on the coordination effect of platinum for use in biolabeling processes.

[0032] (4) The square platinum (II) complex electrochemiluminescent material prepared by this invention helps to solve the ACQ effect in the polymerization amplification process of traditional luminescent materials, has good electrochemiluminescence performance, promotes the development of cathode electrochemiluminescence process, and lays the foundation for the updating and iteration process of commercial luminescent reagents. At the same time, its excellent AIECL performance also broadens the ideas for the design and practical application of signal polymerization amplification strategies. Attached Figure Description

[0033] Figure 1 The image shows a scanning electron microscope (SEM) image of the prismatic platinum(II) complex electrochemiluminescent material prepared in Example 1.

[0034] Figure 2 The structural formula of the prismatic platinum(II) complex and several groups that can replace chlorine atoms are used for biolabeling.

[0035] Figure 3 For homogeneous conditions Ru(bpy)3 2+ Cyclic voltammetry (A) and electrochemiluminescence (B) curves of the platinum(II) complex 1K, and Ru(bpy)3 under heterogeneous conditions. 2+ Cyclic voltammetry (C) and electrochemiluminescence (D) curves of the diatomaceous platinum(II) complex 1K.

[0036] Figure 4 For homogeneous conditions Ru(bpy)3 2+ Cyclic voltammetry (A) and electrochemiluminescence (B) curves of the platinum(II) complex 1K, and Ru(bpy)3 under heterogeneous conditions. 2+ Cyclic voltammetry (C) and electrochemiluminescence (D) curves of the diatomaceous platinum(II) complex 1K. Detailed Implementation

[0037] The following embodiments will further illustrate the technical solution of the present invention in detail. The specific embodiments described herein are merely illustrative and not intended to limit the scope of the invention. Furthermore, the technical features involved in the specific embodiments described below can be combined with each other as long as they do not conflict with each other.

[0038] Example 1

[0039] A method for preparing a square-shaped platinum(II) complex electrochemiluminescent material and its optical performance testing.

[0040] Step 1: Dissolve 2,6-bis(benzimidazole)pyridine (2.00 g, 6.40 mmol) in 10 mL of DMSO solution, slowly add 10 mL of aqueous solution containing potassium hydroxide (1.44 g, 25.70 mmol), and stir until homogeneous;

[0041] Step 2: Purge the solution from Step 1 with nitrogen gas for 10 minutes to remove oxygen from the solvent, then add 10 mL of DMSO solution containing 1,3-propanesulfonic acid lactone (3.14 g, 25.70 mmol) dropwise.

[0042] Step 3: Then reflux at 150℃ for 12 hours. After the reaction is complete, remove water under reduced pressure, then add acetone to precipitate the product, centrifuge to remove the solid, and obtain a yellow solid crude product. Wash with acetone three times and dry to obtain crude product L1.

[0043] Step 4: Dissolve crude product L1 (1.83 g, 2.89 mmol) in 10 mL of DMSO solution, and then add potassium chloroplatinate (0.80 g, 1.92 mmol).

[0044] Step 5: Under a nitrogen atmosphere, reflux at 150°C for 12 hours to obtain a yellow suspension. Then, methanol and acetone are added to completely precipitate the product. After centrifugation to obtain a solid, the solid is washed three times with methanol and acetone in sequence, and dried to obtain the final product 1K.

[0045] The product 1K was observed using a scanning electron microscope, such as... Figure 1 As shown, platinum complexes exhibit a plate-like structure, thus exhibiting a tendency to stack. This leads to the establishment of ground-state non-covalent weak metal-metal and ligand-ligand interactions through the π-electron cloud of the aromatic ring, forming new excited states, such as metal-metal-ligand charge transfer (MMLCT, dσ*→π*) and ligand-metal-metal charge transfer (LMMCT). These charge-transfer (CT) states exhibit absorption and luminescence properties that show a greater magnitude of color shift in size compared to the non-interacting platinum complexes, as well as higher emission quantum yields and longer excited-state lifetimes.

[0046] Step 6: Prepare 0.1 mM Ru(bpy)3Cl2·6H2O and 1 K phosphate buffer solution containing co-reactant TPrA as electrolytes for the three-electrode system. The concentration of TPrA is 25 mM and the concentration of PBS is 0.1 M.

[0047] Electrochemiluminescence (ECL) tests were performed using a three-electrode system. A glassy carbon electrode was used as the working electrode, a platinum electrode as the counter electrode, and an Ag / AgCl electrode as the reference electrode. Cyclic voltammetry was employed, with a potential window of 0V–1.6V (TPrA), a scan rate of 100mV / s, and a PMT excitation voltage of 500V. The test results are as follows: Figure 3 As shown, electrochemical tests were performed under homogeneous conditions using TPRA as a co-reactant. Figure 3 Electrochemiluminescence assays of type A and reduction-oxidation (in the middle) Figure 3 (B) From the cyclic voltammetry plot, a typical oxidation peak belonging to TPRA appears at 0.7 V. In the system containing dissolved 1K, its oxidation potential shifts slightly positively, possibly due to the tendency of 1K molecules to self-assemble in solution, leading to a decrease in the overall conductivity of the solution. Meanwhile, peaks belonging to Ru were observed at 1.3 V and 1.4 V, respectively. 2+ The oxidation peaks at 1K and 1K are observed. The electrochemiluminescence (ECL) plots show that the peak positions of the two substances are close, but due to the higher oxidation potential at 1K, the ECL position is slightly positively shifted, and the ECL intensity is approximately Ru(bpy)3. 2+ 1 / 3 of.

[0048] Example 2

[0049] A method for preparing a square-shaped platinum(II) complex electrochemiluminescent material and its optical performance testing.

[0050] Step 1: Dissolve 2,6-bis(benzimidazole)pyridine (2.00 g, 6.40 mmol) in 10 mL of DMSO solution, slowly add 10 mL of aqueous solution containing potassium hydroxide (1.44 g, 25.70 mmol), and stir until homogeneous;

[0051] Step 2: Purge the solution from Step 1 with nitrogen gas for 10 minutes to remove oxygen from the solvent, then add 10 mL of DMSO solution containing 1,3-propanesulfonic acid lactone (3.14 g, 25.70 mmol) dropwise.

[0052] Step 3: Then reflux at 140℃ for 14 hours. After the reaction is complete, remove water under reduced pressure, then add acetone to precipitate the product, centrifuge to remove the solid, and obtain a yellow solid crude product. Wash with acetone three times and dry to obtain crude product L1.

[0053] Step 4: Dissolve crude product L1 (1.83 g, 2.89 mmol) in 10 mL of DMSO solution, and then add potassium chloroplatinate (0.80 g, 1.92 mmol).

[0054] Step 5: Under a nitrogen atmosphere, reflux at 150°C for 12 hours to obtain a yellow suspension. Then, methanol and acetone are added to completely precipitate the product. After centrifugation to obtain a solid, the solid is washed three times with methanol and acetone in sequence, and dried to obtain the final product 1K.

[0055] Step 6: Prepare 1 mM Ru(bpy)3Cl2·6H2O and 1 K solutions using PBS (0.1 M) buffer solution containing 25 mM TPrA as a co-reactant.

[0056] Step 7: The electrochemiluminescent material is modified onto the working electrode using a drop-coating method. After the pretreated GCE electrode is dried under an infrared lamp, 2.5 μL of a prepared solution of Ru(bpy)3Cl2·6H2O and 1K is dropped onto the electrode surface, followed by drying under an infrared lamp to obtain the modified electrode.

[0057] Electrochemiluminescence (ECL) tests were performed in a three-electrode system. The luminescent material was modified onto the working electrode, with a platinum electrode as the counter electrode and an Ag / AgCl electrode as the reference electrode. Cyclic voltammetry was used, with a potential window of 0V–1.6V (TPrA), a scan rate of 100mV / s, and a PMT excitation voltage of 500V. The test results are as follows: Figure 3 As shown, electrochemical tests were performed in a heterogeneous aggregated state under the condition of using TPRA as a co-reactant. Figure 3 C) and reduction-oxidation type electrochemiluminescence assays ( Figure 3 (D) From the electrochemical data, it can be seen that the oxidation peak position of TprA does not change significantly, proving that the material modification process has no effect on the oxidation process of the co-reactant. Secondly, the analysis of the oxidation peak position of the modified material shows that the peak potential at 1K is slightly reduced, Ru 2+ The oxidation peak potential of 1K showed no significant change, which can be attributed to the modification process reducing the distance between the aggregates of 1K in water and the electrode surface, thus decreasing the energy consumption during diffusion and consequently lowering its oxidation potential. Electrochemiluminescence performance analysis revealed a slight positive shift in the elution position of 1K as the oxidation peak potential advanced. The TPrA positive scan corresponds to the anodic electrochemical signal, indicating that in the aggregated state, the electrochemiluminescence performance of 1K is superior to that of Ru. 2+ The system is about 10 times stronger.

[0058] Example 3

[0059] A method for preparing a square-shaped platinum(II) complex electrochemiluminescent material and its optical performance testing.

[0060] Step 1: Dissolve 2,6-bis(benzimidazole)pyridine (2.00 g, 6.40 mmol) in 10 mL of DMSO solution, slowly add 10 mL of aqueous solution containing potassium hydroxide (1.44 g, 25.70 mmol), and stir until homogeneous;

[0061] Step 2: Purge the solution from Step 1 with nitrogen gas for 10 minutes to remove oxygen from the solvent, then add 10 mL of DMSO solution containing 1,3-propanesulfonic acid lactone (3.14 g, 25.70 mmol) dropwise.

[0062] Step 3: Then reflux at 130℃ for 15 hours. After the reaction is complete, remove water under reduced pressure, then add acetone to precipitate the product, centrifuge to remove the solid, and obtain a yellow solid crude product. Wash with acetone three times and dry to obtain crude product L1.

[0063] Step 4: Dissolve crude product L1 (1.83 g, 2.89 mmol) in 10 mL of DMSO solution, and then add potassium chloroplatinate (0.80 g, 1.92 mmol);

[0064] Step 5: Under a nitrogen atmosphere, reflux at 130°C for 15 hours to obtain a yellow suspension. Then, methanol and acetone are added to completely precipitate the product. After centrifugation to obtain a solid, the solid is washed three times with methanol and acetone in sequence, and dried to obtain the final product 1K.

[0065] Step Six: Prepare 0.1 mM Ru(bpy)3Cl2·6H2O and 1 K phosphate buffer solution containing co-reactant K2S2O8 as electrolytes for the three-electrode system. The concentration of K2S2O8 is 25 mM and the concentration of PBS is 0.1 M.

[0066] Electrochemiluminescence (ECL) was performed using a three-electrode system. A glassy carbon electrode was used as the working electrode, a platinum electrode as the counter electrode, and an Ag / AgCl electrode as the reference electrode. Cyclic voltammetry was employed, with a potential window of 0V to -1.6V (K2S2O8), a scan rate of 100mV / s, and a PMT excitation voltage of 500V. The test results are as follows: Figure 4 The electrochemical tests were performed under homogeneous conditions using K2S2O8 as a co-reactant. Figure 4 Electrochemiluminescence assays of type A and reduction-oxidation (in the middle) Figure 4 (B in the text). Electrochemical data show Ru 2+ The -0.8V peak in the solution and the -1.1V peak in the 1K solution are attributed to the electrochemical reduction of K₂S₂O₈, while no reduction peaks belonging to the luminescent reagent were found. The figure shows that during the electrochemical testing process, Ru(bpy)₃… 2+ The former exhibited only weak electrochemiluminescence, while 1K displayed a bright electrochemiluminescence signal, approximately 35 times stronger. Furthermore, the performance under these conditions was significantly better than that of 1K in the redox system, representing a performance improvement of about 24 times. This is closely related to the reactivity between the material itself and the co-reactant.

[0067] Example 4

[0068] A method for preparing a square-shaped platinum(II) complex electrochemiluminescent material and its optical performance testing.

[0069] Step 1: Dissolve 2,6-bis(benzimidazole)pyridine (2.00 g, 6.40 mmol) in 10 mL of DMSO solution, slowly add 10 mL of aqueous solution containing potassium hydroxide (1.44 g, 25.70 mmol), and stir until homogeneous;

[0070] Step 2: Purge the solution from Step 1 with nitrogen gas for 10 minutes to remove oxygen from the solvent, then add 10 mL of DMSO solution containing 1,3-propanesulfonic acid lactone (3.14 g, 25.70 mmol) dropwise.

[0071] Step 3: Then reflux at 150℃ for 12 hours. After the reaction is complete, remove water under reduced pressure, then add acetone to precipitate the product, centrifuge to remove the solid, and obtain a yellow solid crude product. Wash with acetone three times and dry to obtain crude product L1;

[0072] Step 4: Dissolve crude product L1 (1.83 g, 2.89 mmol) in 10 mL of DMSO solution, and then add potassium chloroplatinate (0.80 g, 1.92 mmol);

[0073] Step 5: Under a nitrogen atmosphere, reflux at 150°C for 12 hours to obtain a yellow suspension. Then, methanol and acetone are added to completely precipitate the product. After centrifugation to obtain a solid, the solid is washed three times with methanol and acetone in sequence, and dried to obtain the final product 1K.

[0074] Step 6: Prepare 1 mM Ru(bpy)3Cl2·6H2O and 1 K solutions using PBS (0.1 M) buffer solution containing 25 mM K2S2O8 as a co-reactant.

[0075] Step 7: The electrochemiluminescent material is modified onto the working electrode using the drop-coating method. After the pretreated GCE electrode is dried under an infrared lamp, 2.5 μL of Ru(bpy)3Cl2·6H2O and 1K of the prepared solution are dropped onto the electrode surface, and then dried under an infrared lamp to obtain the modified electrode.

[0076] Electrochemiluminescence assays were performed using a three-electrode system. The modified electrode served as the working electrode, the platinum electrode as the counter electrode, and the Ag / AgCl electrode as the reference electrode. Cyclic voltammetry was used, with a potential window of 0V to -1.6V (K₂S₂O₈), a scan rate of 100mV / s, and a PMT excitation voltage of 500V. The test results are as follows: Figure 4 The figure shows electrochemical tests performed under heterogeneous aggregation conditions with K2S2O8 as a co-reactant. Figure 4 C) and reduction-oxidation type electrochemiluminescence assays ( Figure 4In the D), the negative scan of K2S2O8 corresponds to the cathodic electrochemical signal, indicating that in the aggregated state, 1K is equivalent to Ru(bpy)3. 2+ The electrochemiluminescence performance is improved by about 16 times.

Claims

1. The application of a water-soluble electrochemiluminescent material based on anion-modified prismatic platinum(II) complex, characterized in that, A three-electrode system was used, comprising the aforementioned prismatic platinum(II) complex electrochemiluminescent material, a working electrode, a counter electrode, a reference electrode, and a co-reactant, to test the electrochemiluminescence performance. The co-reactant was potassium persulfate. The structural formula of the water-soluble anion-modified prismatic platinum(II) complex electrochemiluminescent material is as follows:

Images