A polypeptide fractionation apparatus

Abstract

The application discloses a polypeptide fractionation equipment, which comprises a shell, a partition plate, a pretreatment mechanism, an alarm device, a first membrane filtration assembly and a second membrane filtration assembly; the upper and lower arranged cover plate and the shell are screwed into a hollow cuboid structure, and the partition plate is vertically arranged in the shell and spaced apart left and right, and the shell is divided into a left chamber, a liquid storage chamber and a right chamber from left to right through the partition plate; the pretreatment mechanism is arranged in the left chamber, a chute is arranged on the left partition plate, and mixed polypeptide liquid is delivered to the liquid storage chamber through the chute; the alarm device is arranged in the liquid storage chamber, and a circulating pump, the first membrane filtration assembly, a second pump and the second membrane filtration assembly are sequentially arranged in the right chamber from top to bottom, the circulating pump pumps the mixed polypeptide liquid in the liquid storage chamber into the first membrane filtration assembly for first separation, and then the second pump pumps the mixed polypeptide liquid into the second membrane filtration assembly for second separation. The hollow fiber membrane filtration pipe and the ultrafiltration membrane in the application are different in molecular weight, and the fractionation effect of the mixed polypeptide liquid can be realized.

Description

Technical Field

[0001] This invention relates to the field of membrane separation technology, and more specifically to a polypeptide fractionation and separation device. Background Technology

[0002] Enzymatic hydrolysates contain peptides of varying molecular weights. Studies have shown that peptides below 3000 Da possess biological activity, and the biological activities of peptides with different molecular weights also differ. Therefore, applying membrane filtration to food processing can not only purify the enzymatic hydrolysate but also perform fractionation and separation.

[0003] However, current membrane filtration devices generally suffer from the following drawbacks: 1) They typically use only one type of molecular weight membrane, which cannot effectively separate peptides; 2) When the mixed peptide solution is insufficient, it cannot be detected and replenished in time, thus delaying work and reducing efficiency. Therefore, these problems urgently need to be solved. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a polypeptide fractionation and separation device, which can achieve fractionation and separation of mixed polypeptide liquid by setting hollow fiber membrane filter tube and ultrafiltration membrane with different molecular weights.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: The present invention provides a polypeptide fractionation and separation device, the innovation of which lies in: comprising a shell, a cover plate, partitions, a pretreatment mechanism, an alarm device, a primary membrane filtration assembly, and a secondary membrane filtration assembly; the upper and lower cover plate and the shell are screwed together to form a hollow cuboid structure, and two matching partitions are vertically symmetrically arranged at intervals on both sides in the middle position of the shell, dividing the interior of the shell from left to right into a left chamber, a liquid storage chamber, and a right chamber; a pretreatment mechanism is provided in the upper part of the left chamber, and through… The pretreatment unit filters and stirs the mixed peptide solution; a chute is inclinedly installed through the middle of one of the partitions on the left, and the pretreated mixed peptide solution is transported to the storage chamber through the chute; an alarm device for liquid level indication is installed in the storage chamber, and a circulation pump, a primary membrane filtration assembly, a secondary pump, and a secondary membrane filtration assembly are arranged sequentially from top to bottom in the right chamber. The circulation pump pumps the mixed peptide solution in the storage chamber into the primary membrane filtration assembly for primary separation through the first suction pipe, and then pumps it into the secondary membrane filtration assembly for secondary separation through the secondary pump;

[0006] The pretreatment mechanism includes a filter screen, a fixing block, a limiting plate, a pin, and a handle. A matching filter screen is horizontally positioned slightly above the left chamber, and is horizontally slidably connected to the housing, with its right side abutting against the left side of one of the left partitions. A matching fixing block is horizontally fixed to the left side of the filter screen, and the left side of the fixing block extends horizontally and vertically beyond the left outer side of the housing. Limiting plates are symmetrically positioned horizontally on the upper and lower sides of the fixing block extending beyond the housing, and each limiting plate is fixedly connected to the corresponding outer side of the housing. A pin is vertically positioned in the middle of the upper surface of the upper limiting plate, with its lower end vertically downwards passing through the upper limiting plate, the fixing block, and the lower limiting plate, thus horizontally limiting the filter screen. A handle is vertically positioned on the left side of the fixing block, and the handle is designed to not interfere with the two limiting plates.

[0007] It also includes a motor, coupling, shaft, stirring rod, defoamer, and first guide plate; a matching first guide plate is provided in the left chamber above the chute, and the first guide plate is horizontally inclined downwards towards the chute, the inclination angle of the first guide plate is smaller than the inclination angle of the chute, and the lower end of its upper surface is located at the lower edge of the upper end of the chute; a motor is horizontally spaced between the filter screen and the first guide plate on the left outer side of the housing, the motor is spaced below the limiting plate, and its output end extends horizontally into the left chamber and is linked to the left end of the coupling arranged coaxially; the shaft is horizontally arranged laterally, and its left end is coaxially linked to the right end of the coupling, and its right end is horizontally connected to the coupling. The end is rotatably connected to the left side of the partition plate on the left; several stirring layers are vertically spaced from left to right on the outer circumference of the rotating shaft, and several stirring rods are vertically spaced evenly along the circumference of each stirring layer, with a defoamer fixed on the surface of each stirring rod; the length of each stirring rod gradually increases from left to right, and one end of each stirring rod is screwed to the rotating shaft, while the other end is set to the first guide plate and the filter screen without interfering with each other; driven by the motor, the stirring rods rotate with the rotating shaft, stirring the filtered mixed peptide liquid evenly, and then collecting it at the chute through the first guide plate, and then transporting it to the storage chamber for storage through the chute;

[0008] One end of the first liquid extraction tube is connected to the input end of the circulating pump, and the other end extends into the liquid storage chamber, and then extends vertically downward along a partition on the right to the inner bottom surface of the shell, and is fixedly connected to a partition on the right by several first reinforcing ribs, thereby strengthening and fixing the first liquid extraction tube.

[0009] The alarm device includes an alarm box, an alarm, a battery, a second spring, an upper electrode plate, a lower electrode plate, an elastic rope, and a float. The first liquid extraction tube does not interfere with the alarm action of the alarm device, and a hollow rectangular alarm box is provided on the upper surface of the cover plate relative to the liquid storage cavity. Inside the alarm box, upper and lower electrode plates are horizontally parallel and spaced vertically, with the lower electrode plate fixed to the inner bottom surface of the alarm box. Slider blocks are provided at both ends of the upper electrode plate, and vertically matching slide rails are provided on the left and right inner sidewalls of the alarm box relative to the slider positions. Each slider and slide rail is made of insulating material, and the upper electrode plate is vertically slidably connected to the alarm box through the cooperation of the slider and slide rail. A second spring is vertically installed between the upper surface of the electrode and the inner top surface of the alarm box, and the upper and lower ends of the second spring are fixedly connected to the upper electrode and the alarm box, respectively. A battery is installed on the left outer side of the alarm box, and an alarm is installed on its upper surface. The battery, the upper electrode, the lower electrode, and the alarm are connected by wires to form a circuit. An elastic rope is vertically fixed in the middle of the lower surface of the upper electrode. The lower end of the elastic rope passes vertically downward through the lower electrode, the alarm box, and the cover plate, and extends into the liquid storage cavity, where it is fixedly connected to the upper surface of the float plate horizontally installed in the liquid storage cavity. The elastic rope is made of rubber, and through the cooperation of the float plate, the elastic rope, the upper electrode, the lower electrode, and the second spring, the liquid level of the mixed polypeptide liquid in the liquid storage cavity is monitored and alarmed.

[0010] The primary membrane filtration assembly includes a first inlet pipe, a main pipe, a hollow fiber membrane filter tube, a turbulence roller, a storage tank, a slanted sealing plate, a first drain pipe, and a first solenoid valve. In the upper half of the right chamber, a matching main pipe and storage tank are also arranged vertically at intervals. The main pipe is horizontally spaced below the circulation pump, and the output end of the circulation pump is sealed to the main pipe through the first inlet pipe, pumping the mixed peptide solution in the storage chamber into the main pipe. The upper surface of the storage tank is horizontally inclined downwards towards the right side of the shell, and a [missing information - likely a design feature] is located near the upper end of its upper surface. The container is equipped with an inlet; a first drain pipe is obliquely arranged on the right outer side of the casing relative to the liquid storage tank. The left end of the first drain pipe extends obliquely upward into the right chamber, and the lower end of the upper surface of the liquid storage tank is located at the lower edge of the upper end of the first drain pipe. A first solenoid valve for controlling the on / off state is also provided on the first drain pipe. Several hollow fiber membrane filter tubes are vertically arranged in a matrix at even intervals between the main pipe and the liquid storage tank in the right chamber. The upper end of each hollow fiber membrane filter tube is sealed and connected to the main pipe, and its lower end extends vertically downward. Extending directly above the liquid storage tank and spaced apart from its upper surface; each hollow fiber membrane filter tube is also equipped with horizontally spaced turbulence rollers arranged sequentially from top to bottom, which decelerate the mixed polypeptide liquid flowing through the hollow fiber membrane filter tube; inclined sealing plates are also provided between the lower ends of adjacent hollow fiber membrane filter tubes and between the rightmost row of hollow fiber membrane filter tubes and the right inner side of the shell, each inclined sealing plate being horizontally inclined downwards towards the partition, and the lower end of the leftmost row of inclined sealing plates being spaced apart from the upper surface of the liquid storage tank. A sealing connection is formed, thereby sealing the lower ends of adjacent hollow fiber membrane filter tubes and ensuring that the lower end of each hollow fiber membrane filter tube is not connected to the liquid storage tank. The pore size of each hollow fiber membrane filter tube is 0.1~50 micrometers, and the permeable polypeptide molecules are between 2500~3500 Da. The permeable polypeptide molecules are collected on the inclined sealing plate through the membrane wall of the hollow fiber membrane filter tube, and then guided through the inclined sealing plate to the inlet and stored in the liquid storage tank. The non-permeable polypeptide molecules are collected on the upper surface of the liquid storage tank and discharged through the first drain pipe.

[0011] Preferably, the device further includes a push rod, a first spring, and a locking block; a feed inlet matching the feed pipe is embedded through the upper surface of the cover plate at a position relative to the middle of the left chamber, and grooves are also embedded at intervals on the upper surface of the cover plate relative to the left and right sides of the feed inlet; a matching push rod is vertically provided in each groove, and each push rod slides horizontally in the corresponding groove of the cover plate; a first spring is horizontally provided between the side of each push rod away from the feed inlet and the inner side of the corresponding groove of the cover plate, and the two ends of each first spring are fixedly connected to the corresponding inner side of the cover plate and the corresponding push rod; a locking block is fixedly provided on the side of each push rod near the feed inlet, and locking slots are embedded on the left and right sides of the lower end of the feed pipe relative to the locking block position; the lower end of the feed pipe is vertically inserted into the feed inlet of the cover plate, and the spring force of the first spring abuts and locks the corresponding locking block with the corresponding locking slot of the feed pipe, thereby connecting the feed pipe with the left chamber.

[0012] Preferably, the system further includes a buffer tank, horizontal plates, and a level gauge; a trumpet-shaped buffer tank is horizontally and fixedly attached to the lower surface of the cover plate relative to the feed inlet, the buffer tank being located in the left chamber, with its upper end being the smaller diameter end and sealingly connected to the feed inlet; inside the buffer tank, several horizontal plates are horizontally and parallelly spaced from top to bottom, each horizontal plate matching a corresponding position inside the buffer tank, and each horizontal plate having a length three-quarters of the length of the corresponding position in the buffer tank; the left end face of each horizontal plate located on the left side is fixed to the left inner wall of the buffer tank. The right end face of each of the horizontal plates, which is fixedly connected to the right inner wall of the buffer tank, is fixedly connected to the right side of the buffer tank. The buffer tank is spaced above the pretreatment mechanism, with its large diameter end facing the pretreatment mechanism. The mixed peptide solution enters the buffer tank through the feed pipe, is buffered and slowed down by the horizontal plates, and then flows into the pretreatment mechanism for filtration and stirring. A level gauge is also provided on the upper left inner wall of the shell. The level gauge is located slightly above the horizontal plane where the large diameter end of the buffer tank is located, and the level gauge is used to monitor the amount of mixed peptide solution entering.

[0013] Preferably, the secondary membrane filtration assembly includes a second suction pipe, a second inlet pipe, an ultrafiltration membrane, a second drain pipe, a second solenoid valve, a second guide plate, a third drain pipe, and a third solenoid valve; an ultrafiltration membrane and a second guide plate, matched to it, are also arranged vertically and vertically at intervals relative to the lower part of the secondary pump in the right chamber. The ultrafiltration membrane is horizontally inclined downwards towards the right side of the housing and forms a secondary separation area with the storage tank; a second drain pipe is also inclined on the right outer side of the housing relative to the position of the ultrafiltration membrane. The left end of the second drain pipe extends upwards into the right chamber, and the lower end of the upper surface of the ultrafiltration membrane is located at the lower edge of the upper end of the second drain pipe. A second solenoid valve controlling its on / off state is also provided on the second drain pipe; the second guide plate is horizontally inclined downwards towards the right side of the housing, and a third drain pipe is also inclined on the right outer side of the housing relative to the position of the second guide plate. The left end of the third drain pipe extends obliquely upward into the right chamber, and the lower end of the upper surface of the second guide plate is located at the lower edge of the upper end of the third drain pipe. A third solenoid valve for controlling its on / off state is also provided on the third drain pipe. One end of the second suction pipe is connected to the input end of the secondary pump, and the other end extends upward into the storage tank. It is fixedly connected to the lower surface of the storage tank by several second reinforcing ribs, thereby strengthening and fixing the second suction pipe. The output end of the secondary pump pumps the mixed peptide solution in the storage tank into the ultrafiltration membrane through the second inlet pipe, and performs secondary separation through the ultrafiltration membrane. The pore size of the ultrafiltration membrane is 0.1~50 micrometers, and the peptide molecules that can pass through it are between 1000~1500 Da. The permeable peptide molecules are collected on the second guide plate through the ultrafiltration membrane and then discharged through the third drain pipe, while the non-permeable peptide molecules are discharged through the second drain pipe.

[0014] Preferably, the device further includes electric push rods, casters, and support legs; electric push rods are also vertically and symmetrically arranged at the four right angles of the inner bottom surface of the housing, with two of the electric push rods located in the left chamber and spaced apart below the first guide plate, and the other two electric push rods located in the right chamber and spaced apart below the second guide plate; the telescopic end of each electric push rod extends vertically downward from the lower surface of the housing and is connected to the corresponding caster; support legs are also vertically and symmetrically arranged at the four right angles of the lower surface of the housing, with each support leg located within a square area enclosed by the four casters; when in the upper limit position, the lower end face of each caster is located above the horizontal plane where the lower end of the corresponding support leg is located, and under the drive of the electric push rods, the housing can switch between a moving state and a graded separation state through the cooperation of the casters and support legs.

[0015] The beneficial effects of this invention are:

[0016] (1) The present invention achieves the fractional separation effect of mixed polypeptide liquid by setting hollow fiber membrane filter tube and ultrafiltration membrane with different molecular weights;

[0017] (2) In this invention, the hollow fiber membrane filter tube and the ultrafiltration membrane can be regarded as a series connection. The mixed polypeptide liquid after primary separation has a certain energy after coming out of the hollow fiber membrane filter tube. It is then pumped into the ultrafiltration membrane by the secondary pump for secondary separation, thereby saving energy.

[0018] (3) By setting up an alarm device, the present invention can promptly remind staff to replenish the mixed polypeptide solution when it is insufficient, thereby improving work efficiency;

[0019] (4) The present invention uses a stirring rod and a defoamer together to stir the mixed polypeptide solution evenly while avoiding the generation of bubbles.

[0020] (5) The present invention uses the combination of buffer box and horizontal plate to buffer the mixed polypeptide liquid entering the left chamber, avoid the mixed polypeptide liquid directly impacting the filter screen at high speed, and thus improve the filtration effect.

[0021] (6) The present invention facilitates the switching between the graded separation state and the moving state by using electric push rods, casters and outriggers in combination, thereby improving the stability in the graded separation state.

[0022] (7) The present invention facilitates the quick snap-fit ​​installation of different feed pipes by using the first spring, the locking block and the push rod together, thereby improving work efficiency;

[0023] (8) The present invention improves the primary separation effect by setting up a turbulence roller. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Figure 1 This is a schematic diagram of the structure of a polypeptide fractionation and separation device according to the present invention.

[0025] Among them, 1-shell; 2-cover plate; 3-partition plate; 4-feed pipe; 5-push rod; 6-first spring; 7-clamping block; 8-buffer tank; 9-horizontal plate; 10-level gauge; 11-filter screen; 12-fixing block; 13-limiting plate; 14-pin; 15-handle; 16-motor; 17-coupling; 18-rotating shaft; 19-stirring rod; 20-first guide plate; 21-slide groove; 22-liquid storage chamber; 23-alarm box; 24-alarm; 25-battery; 26-second spring; 27-... 28-Upper electrode plate; 29-Lower electrode plate; 30-Elastic rope; 31-Float plate; 32-First suction pipe; 33-Circulation pump; 34-Main pipe; 35-Hollow fiber membrane filter tube; 36-Storage tank; 37-First drain pipe; 38-Secondary pump; 39-Second suction pipe; 40-Second reinforcing rib; 41-Ultrafiltration membrane; 42-Second drain pipe; 43-Second guide plate; 44-Third drain pipe; 45-Electric push rod; 46-Universal wheel; 47-Outrigger; 48-Break roller; 49-Slanted sealing plate. Detailed Implementation

[0026] The technical solution of the present invention will be clearly and completely described below through specific embodiments.

[0027] The present invention provides a polypeptide fractionation and separation device, comprising a shell 1, a cover plate 2, a partition plate 3, a pretreatment mechanism, an alarm device, a primary membrane filtration assembly, and a secondary membrane filtration assembly; the specific structure is as follows: Figure 1 As shown, the cover plate 2 and the shell 1 are screwed together to form a hollow cuboid structure. Two vertically symmetrical partitions 3 are also provided at intervals in the middle of the shell 1, dividing the interior of the shell 1 from left to right into a left chamber, a storage chamber 22, and a right chamber. A pretreatment mechanism is located slightly above the left chamber, filtering and stirring the mixed polypeptide solution. A chute 21 is obliquely inserted through the lower middle of one of the left partitions 3, transporting the pretreated mixed polypeptide solution to the storage chamber 22. An alarm device for liquid level indication is provided in the storage chamber 22. A circulation pump 32, a primary membrane filter assembly, a secondary pump 37, and a secondary membrane filter assembly are arranged sequentially from top to bottom in the right chamber. The circulation pump 32 pumps the mixed polypeptide solution from the storage chamber 22 into the primary membrane filter assembly for primary separation via the first suction pipe 31, and then pumps it into the secondary membrane filter assembly via the secondary pump 37 for secondary separation.

[0028] The present invention further includes a feed inlet, matching the feed pipe 4, embedded through the upper surface of the cover plate 2 at a position relative to the middle of the left chamber, and grooves are also embedded at intervals on the upper surface of the cover plate 2 on the left and right sides relative to the feed inlet; for example Figure 1As shown, each groove is vertically fitted with a matching push rod 5, and each push rod 5 slides horizontally in the corresponding groove of the cover plate 2. A first spring 6 is horizontally positioned between the side of each push rod 5 away from the feed inlet and the inner side of the corresponding groove of the cover plate 2, and both ends of each first spring 6 are fixedly connected to the corresponding inner side of the cover plate 2 and the corresponding push rod 5, respectively. A locking block 7 is fixedly positioned on the side of each push rod 5 near the feed inlet, and locking slots are respectively embedded on the left and right sides of the lower end of the feed pipe 4 at positions relative to the locking block 7. The lower end of the feed pipe 4 is vertically inserted into the feed inlet of the cover plate 2, and the spring force of the first spring 6 is used to press the corresponding locking block 7 against the corresponding locking slot of the feed pipe 4, thereby connecting the feed pipe 4 with the left chamber.

[0029] In this invention, a trumpet-shaped buffer box 8 is horizontally attached and fixed to the lower surface of the cover plate 2 relative to the feed inlet. Figure 1 As shown, the buffer tank 8 is located in the left chamber, with its upper end being the smaller diameter end, and is sealed and connected to the feed inlet. Inside the buffer tank 8, several horizontal plates 9 are arranged horizontally and alternately from top to bottom. Each horizontal plate 9 matches the corresponding position inside the buffer tank 8, and its length is three-quarters of the length of the corresponding position in the buffer tank 8. The left end face of each horizontal plate 9 located on the left side is fixedly connected to the left inner side wall of the buffer tank 8, and the right end face of each horizontal plate 9 located on the right side is fixedly connected to the right inner side wall of the buffer tank 8. The buffer tank 8 is spaced above the pretreatment mechanism, with its larger diameter end facing the pretreatment mechanism. The mixed peptide solution enters the buffer tank 8 through the feed pipe 4, is buffered and slowed down by the horizontal plates 9, and then flows into the pretreatment mechanism for filtration and stirring. A level gauge 10 is also provided on the upper left inner side wall of the shell 1. The level gauge 10 is located slightly above the horizontal plane where the larger diameter end of the buffer tank 8 is located, and the level gauge 10 is used to monitor the amount of mixed peptide solution entering.

[0030] The pretreatment mechanism includes a filter screen 11, a fixing block 12, a limiting plate 13, a pin 14, a handle 15, a motor 16, a coupling 17, a rotating shaft 18, a stirring rod 19, a defoamer, and a first guide plate 20; for example Figure 1As shown, a matching filter screen 11 is horizontally arranged at the upper part of the left chamber. The filter screen 11 is horizontally and laterally slidably connected to the housing 1, and its right side abuts against the left side of a partition 3 on the left. A matching fixing block 12 is also horizontally fixed on the left side of the filter screen 11, and the left side of the fixing block 12 extends horizontally and vertically out of the left outer side of the housing 1. Limiting plates 13 are also horizontally and symmetrically arranged on the upper and lower sides of the part of the fixing block 12 that extends beyond the housing 1, and each limiting plate 13 is fixedly connected to the corresponding outer side of the housing 1. A pin 14 is also vertically arranged in the middle of the upper surface of the upper limiting plate 13. The lower end of the pin 14 vertically passes through the upper limiting plate 13, the fixing block 12 and the lower limiting plate 13 in sequence, and limits the filter screen 11 in the horizontal direction. A handle 15 is also vertically arranged on the left side of the fixing block 12, and the handle 15 is arranged without interfering with the two limiting plates 13.

[0031] like Figure 1 As shown, a first guide plate 20 is provided in the left chamber above the slide 21, and the first guide plate 20 is horizontally inclined downward towards the slide 21. The inclination angle of the first guide plate 20 is smaller than that of the slide 21, and the lower end of its upper surface is located at the lower edge of the upper end of the slide 21. A motor 16 is horizontally spaced between the filter screen 11 and the first guide plate 20 on the left outer side of the housing 1. The motor 16 is spaced below the limiting plate 13, and its output end extends horizontally into the left chamber and is linked to the left end of the coupling 17 which is coaxially arranged. The rotating shaft 18 is horizontally arranged, and its left end is coaxially linked to the right end of the coupling 17. Its right end is located corresponding to the left side of the partition 3 on the left. Rotary connection; several stirring layers are vertically arranged from left to right on the outer circumference of the rotating shaft 18, and several stirring rods 19 are evenly distributed vertically along the circumference of each stirring layer, and a defoamer is fixed on the surface of each stirring rod 19; the length of each stirring rod 19 gradually increases from left to right, and one end of each stirring rod 19 is screwed to the rotating shaft 18, and the other end is set to the first guide plate 20 and the filter screen 11 without interference; under the drive of the motor 16, the stirring rods 19 rotate with the rotating shaft 18, and after the filtered mixed peptide liquid is stirred evenly, it is collected by the first guide plate 20 to the chute 21, and then transported to the storage chamber 22 for storage through the chute 21.

[0032] like Figure 1 As shown, one end of the first liquid extraction tube 31 is connected to the input end of the circulating pump 32, and the other end extends into the liquid storage chamber 22, and then extends vertically downward along a partition 3 on the right to the inner bottom surface of the housing 1, and is fixedly connected to a partition 3 on the right by several first reinforcing ribs, thereby strengthening and fixing the first liquid extraction tube 31.

[0033] The alarm device of this invention includes an alarm box 23, an alarm 24, a storage battery 25, a second spring 26, an upper electrode plate 27, a lower electrode plate 28, an elastic rope 29, and a float 30; as shown Figure 1 As shown, the first suction pipe 31 does not interfere with the alarm action of the alarm device, and a hollow rectangular alarm box 23 is provided on the upper surface of the cover plate 2 at a position relative to the liquid storage chamber 22; inside the alarm box 23, upper electrode plates 27 and lower electrode plates 28 are horizontally parallel and spaced vertically, and the lower electrode plate 28 is fixedly set on the inner bottom surface of the alarm box 23. Slider blocks are provided at the left and right ends of the upper electrode plate 27, and vertical slide rails matching the sliders are provided on the left and right inner sidewalls of the alarm box 23 at positions relative to the sliders. Each slider and slide rail is made of insulating material, and the upper electrode plate 27 and the alarm box 23 are vertically slidably connected through the cooperation of the sliders and slide rails; a second spring 26 is also vertically provided between the upper surface of the upper electrode plate 27 and the inner top surface of the alarm box 23, and the second spring... The upper and lower ends of 26 are fixedly connected to the upper electrode plate 27 and the alarm box 23, respectively. A storage battery 25 is provided on the left outer side of the alarm box 23, and an alarm 24 is also provided on its upper surface. The storage battery 25, the upper electrode plate 27, the lower electrode plate 28 and the alarm 24 form a circuit through wires. An elastic rope 29 is also vertically fixed in the middle of the lower surface of the upper electrode plate 27. The lower end of the elastic rope 29 passes vertically downward through the lower electrode plate 28, the alarm box 23 and the cover plate 2 in sequence, and extends into the liquid storage chamber 22, and is fixedly connected to the upper surface of the float plate 30 horizontally set in the liquid storage chamber 22. The elastic rope 29 is made of rubber rope, and through the cooperation of the float plate 30, the elastic rope 29, the upper electrode plate 27, the lower electrode plate 28 and the second spring 26, the liquid level of the mixed polypeptide liquid in the liquid storage chamber 22 is monitored and alarmed.

[0034] The primary membrane filtration assembly of this invention includes a first inlet pipe, a main pipe 33, a hollow fiber membrane filter tube 34, a turbulence roller 47, a storage tank 35, a slanted sealing plate 48, a first drain pipe 36, and a first solenoid valve; as shown Figure 1As shown, the upper half of the right chamber is also equipped with a matching main pipe 33 and a storage tank 35 at intervals. The main pipe 33 is horizontally spaced below the circulation pump 32, and the output end of the circulation pump 32 is sealed and connected to the main pipe 33 through the first inlet pipe, pumping the mixed peptide solution in the storage chamber 22 into the main pipe 33. The upper surface of the storage tank 35 is horizontally inclined downwards towards the right side of the shell 1, and an inlet is provided at the upper end of its upper surface. A first drain pipe 36 is also inclined on the right outer side of the shell 1 relative to the position of the storage tank 35. The left end extends upwards into the right chamber at an angle, and the lower end of the upper surface of the liquid storage tank 35 is located at the lower edge of the upper end of the first drain pipe 36. A first solenoid valve controlling the on / off state of the first drain pipe 36 is also provided on the first drain pipe 36. In the right chamber, several hollow fiber membrane filter tubes 34 are vertically arranged in a matrix at even intervals between the main pipe 33 and the liquid storage tank 35. The upper end of each hollow fiber membrane filter tube 34 is sealed and connected to the main pipe 33, and its lower end extends vertically downwards to directly above the liquid storage tank 35, and is spaced apart from the upper surface of the liquid storage tank 35. In each hollow fiber membrane filter tube 34... Inside the hollow fiber membrane filter tube 34, horizontally spaced turbulence rollers 47 are arranged sequentially from top to bottom to slow down the mixed polypeptide solution flowing through the hollow fiber membrane filter tube 34. Inclined sealing plates 48 are also provided between the lower ends of adjacent hollow fiber membrane filter tubes 34 and between the rightmost row of hollow fiber membrane filter tubes 34 and the right inner side of the shell 1. Each inclined sealing plate 48 is horizontally inclined downwards towards the partition plate 3, and the lower end of the leftmost row of inclined sealing plates 48 is sealed to the upper surface of the liquid storage tank 35, thereby sealing the adjacent hollow fiber membrane filter tubes 34. The lower ends are sealed together to ensure that the lower end of each hollow fiber membrane filter tube 34 is not connected to the liquid storage tank 35. The pore size of each hollow fiber membrane filter tube 34 is 0.1~50 micrometers, and the molecular weight of the polypeptides that can pass through it is between 2500~3500 Da. The polypeptide molecules that can pass through are collected on the membrane wall of the hollow fiber membrane filter tube 34 and then flow through the inclined sealing plate 48 to the liquid storage tank 35 for storage. The polypeptide molecules that cannot pass through are collected on the upper surface of the liquid storage tank 35 and then discharged through the first drain pipe 36.

[0035] The secondary membrane filtration assembly of this invention includes a second liquid extraction pipe 38, a second liquid inlet pipe, an ultrafiltration membrane 40, a second liquid outlet pipe 41, a second solenoid valve, a second guide plate 42, a third liquid outlet pipe 43, and a third solenoid valve; as shown Figure 1As shown, in the right chamber, an ultrafiltration membrane 40 and a second guide plate 42, positioned vertically and vertically below the secondary pump 37, are also provided to match it. The ultrafiltration membrane 40 is horizontally inclined downwards towards the right side of the housing 1, forming a secondary separation area with the storage tank 35. A second drain pipe 41 is also inclined on the right outer side of the housing 1 at the position of the ultrafiltration membrane 40. The left end of the second drain pipe 41 extends upwards into the right chamber, and the lower end of the upper surface of the ultrafiltration membrane 40 is located near the lower edge of the upper end of the second drain pipe 41. A second solenoid valve for controlling its on / off state is also provided on the second drain pipe 41. The second guide plate 42 is horizontally inclined downwards towards the right side of the housing 1. A third drain pipe 43 is also inclined on the right outer side of the housing 1 at the position of the second guide plate 42. The left end of the third drain pipe 43 extends upwards into the right chamber, and the lower end of the upper surface of the second guide plate 42 is located near its lower edge. A third solenoid valve is installed at the lower edge of the upper end of the third drain pipe 43 to control its on / off state; one end of the second suction pipe 38 is connected to the input end of the secondary pump 37, and the other end extends upward into the storage tank 35, and is fixedly connected to the lower surface of the storage tank 35 by several second reinforcing ribs 39, thereby strengthening and fixing the second suction pipe 38; the output end of the secondary pump 37 pumps the mixed peptide liquid in the storage tank 35 into the ultrafiltration membrane 40 through the second inlet pipe, and performs secondary separation through the ultrafiltration membrane 40; wherein, the pore size of the ultrafiltration membrane 40 is 0.1~50 micrometers, and the peptide molecular weight that can pass through it is between 1000~1500 Da, thereby collecting the permeable peptide molecules through the ultrafiltration membrane 40 onto the second guide plate 42, and then discharging them through the third drain pipe 43, while the non-permeable peptide molecules are discharged through the second drain pipe 41.

[0036] The present invention also provides vertically symmetrical electric push rods 44 at the four right angles of the inner bottom surface of the housing 1, such as... Figure 1 As shown, two electric push rods 44 are located in the left chamber and spaced apart below the first guide plate 20, while two other electric push rods 44 are located in the right chamber and spaced apart below the second guide plate 42. The telescopic end of each electric push rod 44 extends vertically downward from the lower surface of the housing 1 and is connected to the corresponding universal wheel 45. Vertically symmetrical support legs 46 are also provided at the four right angles of the lower surface of the housing 1, and each support leg 46 is located within a square area enclosed by the four universal wheels 45. When in the upper limit position, the lower end face of each universal wheel 45 is located above the horizontal plane where the lower end of the corresponding support leg 46 is located. Driven by the electric push rods 44, the present invention switches between the moving state and the graded separation state of the housing 1 through the cooperation of the universal wheels 45 and the support legs 46.

[0037] The working principle of this invention includes the following steps;

[0038] (1) First, with the assistance of the operator, move the equipment to the required position, then retract the telescopic end of the electric push rod 44, drive the caster wheel 45 to lift, so that the support leg 46 contacts the ground, ensuring the stability of the equipment in the graded separation process;

[0039] (2) Then push the push rod 5 outward to vertically insert the lower end of the feed pipe 4 into the feed port of the cover plate 2. At this time, release the push rod 5 and, through the spring force of the first spring 6, press the corresponding card block 7 against the corresponding card slot of the feed pipe 4 to connect the feed pipe 4 with the left chamber.

[0040] (3) The mixed polypeptide solution enters the buffer tank 8 through the feed pipe 4, is buffered and decelerated by the horizontal plate 9, and then is filtered by the filter screen 11, stirred by the stirring rod 19, and prevented from generating bubbles by the defoamer.

[0041] (4) The pretreated mixed polypeptide solution flows into the storage chamber 22 through the chute 21 for storage. Then, the circulation pump 32 pumps the mixed polypeptide solution in the storage chamber 22 into the main pipe 33, and then performs primary separation through the hollow fiber membrane filter tube 34. The polypeptide molecules that can pass through the hollow fiber membrane filter tube 34 are collected on the inclined sealing plate 48 through the membrane wall of the hollow fiber membrane filter tube 34, and then flow through the inclined sealing plate 48 to the storage tank 35 for storage. The polypeptide molecules that cannot pass through the membrane are collected on the upper surface of the storage tank 35, and then discharged through the first drain pipe 36.

[0042] (5) The secondary pump 37 pumps the mixed polypeptide liquid in the storage tank 35 into the ultrafiltration membrane 40 for secondary separation. The polypeptide molecules that can pass through the ultrafiltration membrane 40 are collected on the second guide plate 42 and then discharged through the third drain pipe 43, while the polypeptide molecules that cannot pass through the ultrafiltration membrane 40 are discharged through the second drain pipe 41.

[0043] (6) When the mixed polypeptide solution in the storage chamber 22 is insufficient, the liquid level drops. Through the cooperation of the float plate 30 and the elastic rope 29, the upper electrode plate 27 moves downward. When the upper electrode plate 27 contacts the lower electrode plate 28, the alarm 24 sounds an alarm and reminds the user to replenish the mixed polypeptide solution.

[0044] The beneficial effects of this invention are:

[0045] (1) By setting hollow fiber membrane filter tube 34 and ultrafiltration membrane 40, and the two have different molecular weights, the present invention can achieve the effect of fractional separation of mixed polypeptide liquid;

[0046] (2) In this invention, the hollow fiber membrane filter tube 34 and the ultrafiltration membrane 40 can be regarded as a series connection. The mixed polypeptide liquid after primary separation has a certain energy after it comes out of the hollow fiber membrane filter tube 34. Then, it is pumped into the ultrafiltration membrane 40 by the secondary pump 37 for secondary separation, thereby saving energy.

[0047] (3) By setting up an alarm device, the present invention can promptly remind staff to replenish the mixed polypeptide solution when it is insufficient, thereby improving work efficiency;

[0048] (4) By using the stirring rod 19 and the defoamer together, the present invention can not only stir the mixed polypeptide liquid evenly, but also avoid the generation of bubbles.

[0049] (5) The present invention uses the combination of buffer box 8 and horizontal plate 9 to buffer the mixed polypeptide liquid entering the left chamber, thereby preventing the mixed polypeptide liquid from directly impacting the filter screen 11 at high speed, thus improving the filtration effect.

[0050] (6) The present invention facilitates the switching between the graded separation state and the moving state by using the electric push rod 44, the universal wheel 45 and the support leg 46 in combination, thereby improving the stability in the graded separation state.

[0051] (7) The present invention facilitates the quick snap-fit ​​installation of different feed pipes 4 by using the first spring 6, the locking block 7 and the push rod 5 together, thereby improving work efficiency;

[0052] (8) The present invention improves the primary separation effect by setting the turbulence roller 47.

[0053] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the concept and scope of the present invention. Without departing from the design concept of the present invention, all modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope of the present invention. The technical content for which protection is sought in the present invention has been fully described in the technical requirements.

Claims

1. A polypeptide fractionation and separation device, characterized in that: The device includes a shell, a cover plate, partitions, a pretreatment mechanism, an alarm device, a primary membrane filtration assembly, and a secondary membrane filtration assembly. The upper and lower cover plates and the shell are screwed together to form a hollow cuboid structure. Two vertically symmetrical partitions are also arranged at intervals in the middle of the shell, dividing the interior of the shell from left to right into a left chamber, a storage chamber, and a right chamber. A pretreatment mechanism is located slightly above the left chamber, used to filter and stir the mixed polypeptide solution. A chute is obliquely penetrating slightly below the middle of one of the left partitions, used to transport the pretreated mixed polypeptide solution to the storage chamber. An alarm device for liquid level indication is located in the storage chamber. A circulation pump, a primary membrane filtration assembly, a secondary pump, and a secondary membrane filtration assembly are arranged sequentially from top to bottom in the right chamber. The circulation pump pumps the mixed polypeptide solution from the storage chamber into the primary membrane filtration assembly for primary separation via a first suction pipe, and then pumps it into the secondary membrane filtration assembly for secondary separation via the secondary pump. The pretreatment mechanism includes a filter screen, a fixing block, a limiting plate, a pin, and a handle. A matching filter screen is horizontally positioned slightly above the left chamber, and is horizontally slidably connected to the housing, with its right side abutting against the left side of one of the left partitions. A matching fixing block is horizontally fixed to the left side of the filter screen, and the left side of the fixing block extends horizontally and vertically beyond the left outer side of the housing. Limiting plates are symmetrically positioned horizontally on the upper and lower sides of the fixing block extending beyond the housing, and each limiting plate is fixedly connected to the corresponding outer side of the housing. A pin is vertically positioned in the middle of the upper surface of the upper limiting plate, with its lower end vertically downwards passing through the upper limiting plate, the fixing block, and the lower limiting plate, thus horizontally limiting the filter screen. A handle is vertically positioned on the left side of the fixing block, and the handle is designed to not interfere with the two limiting plates. It also includes a motor, coupling, shaft, stirring rod, defoamer, and first guide plate; a matching first guide plate is provided in the left chamber above the chute, and the first guide plate is horizontally inclined downwards towards the chute, the inclination angle of the first guide plate is smaller than the inclination angle of the chute, and the lower end of its upper surface is located at the lower edge of the upper end of the chute; a motor is horizontally spaced between the filter screen and the first guide plate on the left outer side of the housing, the motor is spaced below the limiting plate, and its output end extends horizontally into the left chamber and is linked to the left end of the coupling arranged coaxially; the shaft is horizontally arranged laterally, and its left end is coaxially linked to the right end of the coupling, and its right end is horizontally connected to the coupling. The end is rotatably connected to the left side of the partition plate on the left; several stirring layers are vertically spaced from left to right on the outer circumference of the rotating shaft, and several stirring rods are vertically spaced evenly along the circumference of each stirring layer, with a defoamer fixed on the surface of each stirring rod; the length of each stirring rod gradually increases from left to right, and one end of each stirring rod is screwed to the rotating shaft, while the other end is set to the first guide plate and the filter screen without interfering with each other; driven by the motor, the stirring rods rotate with the rotating shaft, stirring the filtered mixed peptide liquid evenly, and then collecting it at the chute through the first guide plate, and then transporting it to the storage chamber for storage through the chute; One end of the first liquid extraction tube is connected to the input end of the circulating pump, and the other end extends into the liquid storage chamber, and then extends vertically downward along a partition on the right to the inner bottom surface of the shell, and is fixedly connected to a partition on the right by several first reinforcing ribs, thereby strengthening and fixing the first liquid extraction tube. The alarm device includes an alarm box, an alarm, a battery, a second spring, an upper electrode plate, a lower electrode plate, an elastic rope, and a float. The first liquid extraction tube does not interfere with the alarm action of the alarm device, and a hollow rectangular alarm box is provided on the upper surface of the cover plate relative to the liquid storage cavity. Inside the alarm box, upper and lower electrode plates are horizontally parallel and spaced vertically, with the lower electrode plate fixed to the inner bottom surface of the alarm box. Slider blocks are provided at both ends of the upper electrode plate, and vertically matching slide rails are provided on the left and right inner sidewalls of the alarm box relative to the slider positions. Each slider and slide rail is made of insulating material, and the upper electrode plate is vertically slidably connected to the alarm box through the cooperation of the slider and slide rail. A second spring is vertically installed between the upper surface of the electrode and the inner top surface of the alarm box, and the upper and lower ends of the second spring are fixedly connected to the upper electrode and the alarm box, respectively. A battery is installed on the left outer side of the alarm box, and an alarm is installed on its upper surface. The battery, the upper electrode, the lower electrode, and the alarm are connected by wires to form a circuit. An elastic rope is vertically fixed in the middle of the lower surface of the upper electrode. The lower end of the elastic rope passes vertically downward through the lower electrode, the alarm box, and the cover plate, and extends into the liquid storage cavity, where it is fixedly connected to the upper surface of the float plate horizontally installed in the liquid storage cavity. The elastic rope is made of rubber, and through the cooperation of the float plate, the elastic rope, the upper electrode, the lower electrode, and the second spring, the liquid level of the mixed polypeptide liquid in the liquid storage cavity is monitored and alarmed. The primary membrane filtration assembly includes a first inlet pipe, a main pipe, a hollow fiber membrane filter tube, a turbulence roller, a storage tank, a slanted sealing plate, a first drain pipe, and a first solenoid valve. In the upper half of the right chamber, a matching main pipe and storage tank are also arranged vertically at intervals. The main pipe is horizontally spaced below the circulation pump, and the output end of the circulation pump is sealed to the main pipe through the first inlet pipe, pumping the mixed peptide solution in the storage chamber into the main pipe. The upper surface of the storage tank is horizontally inclined downwards towards the right side of the shell, and a [missing information - likely a design feature] is located near the upper end of its upper surface. The container is equipped with an inlet; a first drain pipe is obliquely arranged on the right outer side of the casing relative to the liquid storage tank. The left end of the first drain pipe extends obliquely upward into the right chamber, and the lower end of the upper surface of the liquid storage tank is located at the lower edge of the upper end of the first drain pipe. A first solenoid valve for controlling the on / off state is also provided on the first drain pipe. Several hollow fiber membrane filter tubes are vertically arranged in a matrix at even intervals between the main pipe and the liquid storage tank in the right chamber. The upper end of each hollow fiber membrane filter tube is sealed and connected to the main pipe, and its lower end extends vertically downward. Extending directly above the liquid storage tank and spaced apart from its upper surface; each hollow fiber membrane filter tube is also equipped with horizontally spaced turbulence rollers arranged sequentially from top to bottom, which decelerate the mixed polypeptide liquid flowing through the hollow fiber membrane filter tube; inclined sealing plates are also provided between the lower ends of adjacent hollow fiber membrane filter tubes and between the rightmost row of hollow fiber membrane filter tubes and the right inner side of the shell, each inclined sealing plate being horizontally inclined downwards towards the partition, and the lower end of the leftmost row of inclined sealing plates being spaced apart from the upper surface of the liquid storage tank. A sealing connection is formed, thereby sealing the lower ends of adjacent hollow fiber membrane filter tubes and ensuring that the lower end of each hollow fiber membrane filter tube is not connected to the liquid storage tank. The pore size of each hollow fiber membrane filter tube is 0.1~50 micrometers, and the permeable polypeptide molecules are between 2500~3500 Da. The permeable polypeptide molecules are collected on the inclined sealing plate through the membrane wall of the hollow fiber membrane filter tube, and then guided through the inclined sealing plate to the inlet and stored in the liquid storage tank. The non-permeable polypeptide molecules are collected on the upper surface of the liquid storage tank and discharged through the first drain pipe.

2. The polypeptide fractionation and separation device according to claim 1, characterized in that: It also includes a push rod, a first spring, and a locking block; a feed inlet matching the feed pipe is embedded through the upper surface of the cover plate at a position relative to the middle of the left chamber, and grooves are also embedded at intervals on the upper surface of the cover plate relative to the left and right sides of the feed inlet; a matching push rod is vertically provided in each groove, and each push rod slides horizontally in the corresponding groove of the cover plate; a first spring is horizontally provided between the side of each push rod away from the feed inlet and the inner side of the corresponding groove of the cover plate, and the two ends of each first spring are fixedly connected to the corresponding inner side of the cover plate and the corresponding push rod; a locking block is fixedly provided on the side of each push rod near the feed inlet, and locking slots are embedded on the left and right sides of the lower end of the feed pipe relative to the locking block position; the lower end of the feed pipe is vertically inserted into the feed inlet of the cover plate, and the spring force of the first spring abuts and locks the corresponding locking block and the corresponding locking slot of the feed pipe, thereby connecting the feed pipe to the left chamber.

3. The polypeptide fractionation and separation device according to claim 2, characterized in that: It also includes a buffer tank, horizontal plates, and a level gauge; a trumpet-shaped buffer tank is horizontally and fixedly attached to the lower surface of the cover plate relative to the feed inlet, the buffer tank is located in the left chamber, and its upper end is the smaller diameter end, which is sealed and connected to the feed inlet; inside the buffer tank, several horizontal plates are horizontally and parallelly spaced from top to bottom, each horizontal plate matching the corresponding position inside the buffer tank, and its length is three-quarters of the length of the corresponding position of the buffer tank. The left end face of each horizontal plate located on the left side is fixedly connected to the left inner side wall of the buffer tank. The right end face of each of the horizontal plates located on the right side is fixedly connected to the inner right side wall of the buffer tank. The buffer tank is spaced apart and positioned directly above the pretreatment mechanism, with its large diameter end facing the pretreatment mechanism. The mixed peptide solution enters the buffer tank through the feed pipe, is buffered and slowed down by the horizontal plates, and then flows into the pretreatment mechanism for filtration and stirring. A level gauge is also provided on the upper left inner side wall of the shell. The level gauge is located slightly above the horizontal plane where the large diameter end of the buffer tank is located, and the level gauge is used to monitor the amount of mixed peptide solution entering.

4. The polypeptide fractionation and separation device according to claim 1, characterized in that: The secondary membrane filtration assembly includes a second suction pipe, a second inlet pipe, an ultrafiltration membrane, a second drain pipe, a second solenoid valve, a second guide plate, a third drain pipe, and a third solenoid valve. In the right chamber, an ultrafiltration membrane and a second guide plate are also arranged vertically and vertically at intervals relative to the secondary pump. The ultrafiltration membrane is horizontally inclined downwards towards the right side of the housing and forms a secondary separation area with the storage tank. A second drain pipe is also inclined on the right outer side of the housing relative to the ultrafiltration membrane. The left end of the second drain pipe extends upwards into the right chamber, and the lower end of the upper surface of the ultrafiltration membrane is located at the lower edge of the upper end of the second drain pipe. A second solenoid valve is also provided on the second drain pipe to control its on / off state. The second guide plate is horizontally inclined downwards towards the right side of the housing, and a third drain pipe is also inclined on the right outer side of the housing relative to the second guide plate. The left end of the drain pipe extends upward at an angle into the right chamber, and the lower end of the upper surface of the second guide plate is located at the lower edge of the upper end of the third drain pipe. A third solenoid valve for controlling its on / off state is also provided on the third drain pipe. One end of the second suction pipe is connected to the input end of the secondary pump, and the other end extends upward into the storage tank. It is fixedly connected to the lower surface of the storage tank by several second reinforcing ribs, thereby strengthening and fixing the second suction pipe. The output end of the secondary pump pumps the mixed peptide solution in the storage tank into the ultrafiltration membrane through the second inlet pipe, and performs secondary separation through the ultrafiltration membrane. The pore size of the ultrafiltration membrane is 0.1~50 micrometers, and the peptide molecules that can pass through it are between 1000~1500 Da. The permeable peptide molecules are collected on the second guide plate through the ultrafiltration membrane and then discharged through the third drain pipe, while the non-permeable peptide molecules are discharged through the second drain pipe.

5. The polypeptide fractionation and separation device according to claim 4, characterized in that: It also includes electric push rods, casters, and support legs; electric push rods are vertically and symmetrically arranged at the four right angles of the inner bottom surface of the housing, with two of the electric push rods located in the left chamber and spaced apart below the first guide plate, and the other two electric push rods located in the right chamber and spaced apart below the second guide plate; the telescopic end of each electric push rod extends vertically downward from the lower surface of the housing and is connected to the corresponding caster; support legs are vertically and symmetrically arranged at the four right angles of the lower surface of the housing, with each support leg located within a square area enclosed by the four casters; when in the upper limit position, the lower end face of each caster is located above the horizontal plane where the lower end of the corresponding support leg is located, and under the drive of the electric push rods, the housing can switch between a moving state and a graded separation state through the cooperation of the casters and support legs.

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