Taraxacum mongolicum Tmhy5 gene, expression protein and application thereof
CN116987712BActive Publication Date: 2026-07-14INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
- Filing Date
- 2023-08-17
- Publication Date
- 2026-07-14
AI Technical Summary
Technical Problem
The biological function of the HY5 gene in dandelion is still unclear, and existing technologies cannot effectively improve the quality of dandelion, especially the content of chicoric acid.
Method used
The overexpression vector pRI101-TmHY5-GFP of the dandelion TmHY5 gene was cloned and constructed, and transformed into dandelion. The gene promoted the synthesis of chicoric acid by transcribed activation of chicoric acid synthase genes such as PAL1, PAL3, PAL4, C4H1, 4CL1 and HQT2.
Benefits of technology
The chicoric acid content in transgenic dandelion was significantly increased, and a new high-chicoric acid dandelion germplasm was obtained.
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Figure CN116987712B_ABST
Abstract
The present application discloses Taraxacum mongolicum TmHY5 gene, expression protein and application thereof, and belongs to the technical field of plant molecular biology.The nucleotide sequence of the Taraxacum mongolicum TmHY5 gene disclosed by the present application is shown as SEQ ID NO.1.The present application takes Taraxacum mongolicum leaves as materials, obtains the Taraxacum mongolicum TmHY5 gene through cloning, constructs an overexpression vector pRI101-TmHY5-GFP on the basis, and is transferred into Taraxacum mongolicum to obtain transgenic plants.The chicoric acid content analysis result shows that the chicoric acid content of the transgenic Taraxacum mongolicum is significantly higher than that of the control group;the gene function identification result shows that the TmHY5 gene activates the expression of the Tm4CL1 gene in Taraxacum mongolicum to increase the chicoric acid content.The TmHY5 gene is an important gene for Taraxacum mongolicum to carry out genetic engineering and improved breeding.
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