Scarless wound healing material, preparation method and application thereof

Abstract

The application relates to a scarless wound healing promoting material, a preparation method and application, and belongs to the field of functional composite materials. The material is prepared by blending a tissue adhesive, a biological polypeptide and a conductive material; the tissue adhesive is a polyurethane adhesive; the biological polypeptide is one or a mixture of two or more of soybean peptides, acetyl hexapeptide-8, corn polypeptides and palmitoyl tripeptide-1; and the conductive material is one or a mixture of two or more of graphite, nano-silver powder and gallium-indium alloy. The scarless wound healing promoting material has excellent biocompatibility, tissue adhesion and skin compliance, has the functions of hemostasis, leakage prevention and antibiosis, can be widely applied to the field of medical materials, plays an active role in wound repair under the assistance of electric stimulation, effectively activates hair follicle regeneration, and can be used for scar removal adhesive and other scar prevention medical materials for mediating scarless repair.

Description

Technical Field

[0001] This invention belongs to the field of functional composite materials, specifically relating to a scarless wound healing promoting material, its preparation method, and its application. Background Technology

[0002] The skin is the largest organ in the human body, protecting internal tissues and organs from harmful external factors and playing a vital role in sensory and metabolic functions. However, due to factors such as diabetes, aging, and burns, skin or tissue injuries often fail to heal for extended periods. Therefore, research on promoting wound healing is an important topic in life sciences and for improving the quality of human life.

[0003] During skin healing, the proliferation of myofibroblasts at the wound site, while repairing the wound, can lead to scar tissue formation and, in severe cases, tissue dysfunction, resulting in both physical and psychological burdens. Studies have shown that regenerated hair follicles at the wound site can transform myofibroblasts into adipocytes, preventing excessive dermal fibrosis and thus achieving scarless wound healing. Therefore, activating hair follicles at the wound site helps achieve scarless skin repair. Appropriate exogenous electrical stimulation can activate human dermal papilla cells, playing a positive role in hair regeneration. Simultaneously, electrical stimulation helps accelerate cell migration and proliferation, contributing positively to wound healing. This study promotes rapid, scarless skin repair by activating wound hair follicles with adjuvant exogenous electrical stimulation, showing great promise for wound scar intervention and treatment. However, traditional electrical stimulation methods rely on large-scale electrical stimulation devices, which are expensive, involve cumbersome treatment processes, and the rigid electrodes are difficult to adhere well to the skin surface, hindering wound recovery. The paper Int. J. Mol. Sci. 2023, 24, 10986 describes the effective prevention and treatment of scars already formed during the healing stage using capacitive resistive electrotransfer therapy (CRET). However, the clinical device used in this study is large, cumbersome, expensive, and extremely inconvenient for daily use.

[0004] Tissue adhesives can adhere to tissue surfaces through covalent cross-linking reactions with functional groups or through non-covalent interactions such as hydrogen bonding and hydrophobic interactions, providing a better option for wound treatment and postoperative repair. Currently, there are no reports on tissue adhesives that promote scarless wound healing through electrical stimulation. Therefore, a medical material with good biocompatibility, tissue adhesion, skin compliance, and uniform electrical response to mediate scarless skin healing urgently needs to be developed. Summary of the Invention

[0005] To address the problems existing in the prior art, this invention provides a scarless wound healing promoting material, its preparation method, and its applications. The composite material obtained by this invention has uniform conductivity, good biocompatibility, and is safe and non-toxic. It can significantly promote wound repair through the auxiliary effect of external electrical stimulation and can also promote hair follicle regeneration to mediate scarless skin repair.

[0006] The technical solution of the present invention:

[0007] A scarless wound healing promoting material, wherein the material is prepared by blending a tissue adhesive, a bioactive peptide, and a conductive material; the tissue adhesive is a polyurethane adhesive; the bioactive peptide is one or more of soybean peptide, acetyl hexapeptide-8, corn peptide, and palmitoyl tripeptide-1; and the conductive material is one or more of graphite, nano-silver powder, and gallium indium alloy.

[0008] Furthermore, the amount of each substance added is calculated based on 100 parts by weight of tissue adhesive, 600-1500 parts by weight of conductive material, and 5-30 parts by weight of biopeptides.

[0009] Furthermore, in the scarless wound healing promoting material, the conductive material has conductivity and antibacterial properties, the tissue adhesive has tissue adhesion and helps stop wound bleeding, and the bioactive peptides improve the wettability of metals, enhance interfacial interactions, and have anti-inflammatory effects.

[0010] Furthermore, the tissue adhesive is prepared by solution reaction of raw materials comprising the following components: 100 parts by weight of isocyanate, 300-600 parts by weight of polyol mixture, 15-55 parts by weight of chain extender, 2-10 parts by weight of catalyst, and 500-1000 parts by weight of solvent.

[0011] Furthermore, the isocyanate is one or a mixture of two or more of isophorone diisocyanate, dicyclohexylmethane-4,4'-diisocyanate, and diphenylmethane diisocyanate.

[0012] Furthermore, the polypolyol mixture is a mixture of two or more of polyether diol 400-3000 or polyester diol 400-3000; the chain extender is one of dihydrazide carbonate, oxaloyl dihydrazide, heptane dihydrazide, and dodecane dicarboxylic acid dihydrazide; the catalyst is an organotin catalyst; and the solvent is dimethylformamide.

[0013] Furthermore, the preparation process of the tissue adhesive is as follows: the polyol mixture, catalyst and isocyanate are dissolved in the solvent in proportion, the temperature is raised to 60℃-80℃, and the reaction is stirred for 2-3 hours; after cooling to 10℃-30℃, the chain extender is added, and the reaction is stirred at room temperature for 2-4 hours; after removing the solvent, it is dissolved in anhydrous ethanol solution for later use.

[0014] A method for preparing a scarless wound healing promoting material includes the following steps: dispersing a conductive material in an alcohol-water solution of dissolved biological polypeptides using ultrasound for 10-120 min; adding anhydrous ethanol solution of tissue adhesive according to the specified ratio and ultrasound for 1-3 min; pouring the dispersion into a mold or scraping it into a film, and drying it to obtain the scarless wound healing promoting material.

[0015] Application of a scarless wound healing promoting material, wherein the material is non-biotoxic, has tissue adhesion and skin compliance, can prevent wound dehiscence through effective adhesion, and has hemostatic, anti-leakage and antibacterial functions, and can be widely used in the field of medical materials.

[0016] Furthermore, with the assistance of electrical stimulation, hair follicle regeneration can be activated, reducing scar formation. This can be used in scar removal adhesives that mediate scarless repair and other medical materials for scar prevention.

[0017] The present invention has the following beneficial effects:

[0018] (1) The material of the present invention has excellent biocompatibility and no biotoxicity, and can achieve long-term adhesion and stable treatment of human tissues.

[0019] (2) The tissue adhesive matrix of the present invention has good tissue adhesion, can be tightly bonded to the skin in both dry and wet conditions, and can be applied to joints and other parts of the body, which is beneficial for portable treatment.

[0020] (3) The biopeptides of the present invention can improve the wettability of conductive materials, enhance the interface interaction with the matrix, facilitate the uniform distribution of electrical stimulation, and at the same time help improve the immune function of the wound and help maintain the healthy state of skin fibers, thereby assisting in the repair of wound skin.

[0021] (4) The conductive material in this invention is non-toxic and has good conductivity and antibacterial properties, which helps to keep the wound sterile and facilitate the electrical stimulation treatment of the wound. Attached Figure Description

[0022] Figure 1 The image shows a cross-sectional X-ray energy spectrum of the scarless wound healing promoting material in Example 1 (white dots represent gallium elements);

[0023] Figure 2This is a schematic diagram of the scarless wound healing promoting material adhering to the skin in Example 1;

[0024] Figure 3 Images of H&E staining of wounds from the material in Example 1 after 9 days. Specific implementation methods

[0025] Unless otherwise specified, all reagents used in the embodiments of this invention can be purchased commercially.

[0026] It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the invention. In addition to the raw materials used in the embodiments of this invention, any raw material components that contain the same functional groups or include the same structural units involved in this invention, and which are replaced by equivalent substitutions, should be included within the scope of protection of this invention. The invention will be further described below with reference to specific embodiments.

[0027] The present invention provides accompanying drawings of detection results for some embodiments. Other embodiments and comparative examples use the same detection method. Those skilled in the art can directly and without doubt determine the content of the embodiments of the present invention using the detection method provided by the present invention.

[0028] The external electrical stimulation method used in this invention is to insert a 3.6V button battery into a material that mediates scarless wound healing.

[0029] The present invention will be further described below with reference to the embodiments.

[0030] Example 1

[0031] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of isophorone diisocyanate, 480 parts of polycarbonate diol 2000, 5 parts of polyethylene glycol 400, and 5 parts of dibutyltin disilicate. Add 600 parts of N,N-dimethylformamide to dissolve in a three-necked flask, heat to 60°C, and stir for 3 hours. Stop heating and allow to cool naturally to room temperature. Add 40 parts of dihydrazide carbonate and stir for 2 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 1, and dissolve it in 10 mL of anhydrous ethanol solution for later use.

[0032] (2) Preparation of scarless wound healing promoting material: 1500 parts by weight of gallium-indium alloy were dispersed in 20 ml of alcohol-water solution containing 30 parts by weight of soybean peptide using ultrasonic pulverization, and ultrasonicated for 60 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 1. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0033] Example 2

[0034] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of dicyclohexylmethane-4,4'-diisocyanate, 480 parts of polycarbonate diol 2000, 5 parts of polyethylene glycol 400, and 3 parts of dibutyltin disilicate. Place them in a three-necked flask and add 800 parts of N,N-dimethylformamide to dissolve them. Heat the mixture to 75°C and stir for 2.5 h. Stop heating and allow it to cool naturally to room temperature. Then add 40 parts of dihydrazide carbonate and stir for 3 h at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 2, and dissolve it in 10 mL of anhydrous ethanol solution for later use.

[0035] (2) Preparation of scarless wound healing promoting material: 1000 parts by weight of gallium-indium alloy were dispersed in 20 ml of alcohol-water solution containing 25 parts by weight of soybean peptide using ultrasonic pulverization, and ultrasonicated for 60 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 2. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0036] Example 3

[0037] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of isophorone diisocyanate, 400 parts of polyethylene glycol 2000, 20 parts of polycarbonate diol 400, and 2 parts of dibutyltin disilicate. Place them in a three-necked flask and add 500 parts of N,N-dimethylformamide to dissolve them. Heat to 80°C and stir for 2 hours. Stop heating and allow to cool naturally to room temperature. Add 30 parts of dihydrazide carbonate and stir for 3 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 3, which is then dissolved in 10 mL of anhydrous ethanol solution for later use.

[0038] (2) Preparation of scarless wound healing promoting material: 1000 parts by weight of nano-silver powder was dispersed in 20 ml of alcohol-water solution containing 25 parts by weight of soybean peptide using ultrasonic pulverization, and ultrasonicated for 100 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 3. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0039] Example 4

[0040] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of diphenylmethane diisocyanate, 400 parts of polycarbonate diol 2000, 20 parts of polyethylene glycol 400, and 2 parts of dibutyltin disilicate. Add 600 parts of N,N-dimethylformamide to dissolve in a three-necked flask, heat to 80°C, and stir for 3 hours. Stop heating and allow to cool naturally to room temperature. Add 30 parts of heptane dihydrazine and stir for 4 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 4, and dissolve it in 10 mL of anhydrous ethanol solution for later use.

[0041] (2) Preparation of scarless wound healing promoting material: 1200 parts by weight of nano-silver powder was dispersed in 20 ml of alcohol-water solution containing 25 parts by weight of soybean peptide using ultrasonic pulverization, and ultrasonicated for 100 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 4. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0042] Example 5

[0043] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of dicyclohexylmethane-4,4'-diisocyanate, 320 parts of polycarbonate diol 2000, 27 parts of polyethylene glycol 400, and 2 parts of dibutyltin disilicate. Add 500 parts of N,N-dimethylformamide to dissolve in a three-necked flask, heat to 75°C, and stir for 2.5 h. Stop heating and allow to cool naturally to room temperature. Add 40 parts of dihydrazide carbonate and stir for 3 h at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 5, and dissolve it in 10 mL of anhydrous ethanol solution for later use.

[0044] (2) Preparation of scarless wound healing promoting material: 1200 parts by weight of nano silver powder was dispersed in 20 ml of alcohol-water solution containing 20 parts by weight of soybean peptide using ultrasonic pulverization, and ultrasonicated for 80 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 5. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0045] Example 6

[0046] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of dicyclohexylmethane-4,4'-diisocyanate, 320 parts of polycarbonate diol 2000, 27 parts of polyethylene glycol 400, and 2 parts of dibutyltin disilicate. Add 700 parts of N,N-dimethylformamide to dissolve in a three-necked flask, heat to 70°C, and stir for 3 hours. Stop heating and allow to cool naturally to room temperature. Add 55 parts of dodecanedicarboxylic acid dihydrazide and stir for 3 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 6, and dissolve it in 10 mL of anhydrous ethanol solution for later use.

[0047] (2) Preparation of scarless wound healing promoting material: 1200 parts by weight of nano-silver powder were dispersed in 20 ml of alcohol-water solution containing 15 parts by weight of soybean peptides by ultrasonic pulverization, and ultrasonicated for 120 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 6. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0048] Example 7

[0049] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of dicyclohexylmethane-4,4'-diisocyanate, 280 parts of polyethylene glycol 2000, 20 parts of polyethylene glycol 400, and 2 parts of dibutyltin disilicate. Place them in a three-necked flask and add 500 parts of N,N-dimethylformamide to dissolve them. Heat the mixture to 70°C and stir for 3 hours. Stop heating and allow it to cool naturally to room temperature. Then add 40 parts of dihydrazide carbonate and stir for 3 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 7, which is then dissolved in 10 mL of anhydrous ethanol solution for later use.

[0050] (2) Preparation of scarless wound healing promoting material: 1000 parts by weight of nano silver powder was dispersed in 20 ml of alcohol-water solution containing 10 parts by weight of acetyl hexapeptide-8 using ultrasonic pulverization, and ultrasonicated for 40 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 7. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0051] Example 8

[0052] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of dicyclohexylmethane-4,4'-diisocyanate, 280 parts of polycarbonate diol 2000, 20 parts of polyethylene glycol 400, and 2 parts of dibutyltin disilicate. Add 1000 parts of N,N-dimethylformamide to dissolve in a three-necked flask, heat to 70°C, and stir for 3 hours. Stop heating and allow to cool naturally to room temperature. Add 15 parts of dihydrazide carbonate and stir for 2 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 8, and dissolve it in 10 mL of anhydrous ethanol solution for later use.

[0053] (2) Preparation of scarless wound healing promoting material: 1000 parts by weight of graphite were dispersed in 20 ml of alcohol-water solution containing 5 parts by weight of palmitoyl tripeptide-1 by ultrasonic pulverization and ultrasonicated for 40 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 8. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0054] Example 9

[0055] (1) Preparation of tissue adhesive: Weigh 100 parts by weight of isophorone diisocyanate, 520 parts of polyethylene glycol 3000, 80 parts of polyethylene glycol 400, and 10 parts of dibutyltin disilicate. Place them in a three-necked flask and add 1000 parts of N,N-dimethylformamide to dissolve them. Heat the mixture to 60°C and stir for 3 hours. Stop heating and allow it to cool naturally to room temperature. Then add 40 parts of oxaloyl dihydrazine and stir for 3 hours at room temperature. Dry the N,N-dimethylformamide to obtain tissue adhesive 9, which is then dissolved in 10 mL of anhydrous ethanol solution for later use.

[0056] (2) Preparation of scarless wound healing promoting material: 1000 parts by weight of nano-silver powder was dispersed in 20 ml of alcohol-water solution containing 22 parts by weight of corn polypeptide using ultrasonic pulverization, and ultrasonicated for 40 min; polyurethane-ethanol solution (100 parts by weight of polyurethane) prepared in step (1) was added according to the ratio, and ultrasonicated for 2 min; the dispersion was poured into a mold and dried to obtain scarless wound healing promoting material 9. A 3.6V button battery was fixed at the wound site of mice to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration status. The activation status is shown in Table 2.

[0057] Comparative Example 1

[0058] Keeping all other aspects of Example 1(1) unchanged, step (2) did not add soybean peptides. Instead, the nano-silver powder was directly sonicated in a polyurethane ethanol solution for 62 minutes. The dispersion was then poured into a mold and dried to obtain the product. A 3.6V button battery was used to fix the mouse wound to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe the hair follicle regeneration. The activation status is shown in Table 2.

[0059] Comparative Example 2

[0060] Keeping all other aspects of Example 1(1) unchanged, the prepared and dried product was directly applied to the wound of mice without connecting a button battery. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The activation status is shown in Table 2.

[0061] Comparative Example 3

[0062] Thin copper sheets were used as electrode adhesives, and a 3.6V button battery was used to fix the electrodes at the mouse wound site to apply electrical stimulation to activate hair follicle regeneration and promote healing. The wound recovery status is shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe the hair follicle regeneration and activation status, as shown in Table 2.

[0063] Table 1 Wound healing rate

[0064]

[0065]

[0066] Table 2 Hair follicle regeneration status (9 days)

[0067] Is hair follicle regeneration possible? Example 1 yes Example 2 yes Example 3 yes Example 4 yes Example 5 yes Example 6 yes Example 7 yes Example 8 yes Example 9 yes Comparative Example 1 no Comparative Example 2 no Comparative Example 3 no

[0068] Comparing Tables 1 and 2, Comparative Example 1, lacking the addition of bioactive peptides, showed slower wound healing and no hair follicle regeneration after 9 days, demonstrating that peptide addition enhances the wettability of the conductive filler, improves skin immunity, and promotes better wound repair and hair follicle regeneration. Comparative Example 2, without electrical stimulation, also showed slower wound healing and no new hair follicle generation, proving that electrical stimulation promotes wound repair and hair follicle activation, contributing to scarless repair. Comparative Example 3, using copper sheets as electrodes, is rigid, hard, and lacks tissue adhesion, making it difficult to achieve good close contact with the skin and uniform electrical stimulation treatment. Therefore, it cannot achieve good wound repair, let alone hair follicle regeneration and activation.

[0069] Figure 1 The energy dispersive spectroscopy (EDS) images show that the metal elements in the gallium-indium alloy in Example 1 do not aggregate over a large area in the scarless wound healing material, proving that soybean peptides are beneficial for achieving uniform dispersion of fillers and for achieving uniform and stable electrical stimulation. Figure 2 Scarless wound healing materials can achieve good adhesion to the skin surface, and the adhesive does not fall off when gripped, indicating that they have good bioadhesion and skin compliance, which is beneficial for daily wound treatment. Figure 3 H&E staining of the healed wound site revealed clearly newly formed hair follicles, proving that Example 1 successfully activated the wound hair follicles through electrical stimulation, thereby achieving scarless wound healing.

[0070] The scarless wound healing promoting material provided by this invention can activate hair follicle regeneration while promoting healing, mediating scarless wound repair. It also has good tissue adhesion, skin suppleness, and biocompatibility, overcoming the difficulty of traditional adhesive materials in achieving scarless repair. It has the potential to achieve more profound applications in challenging medical fields such as burns and deformities, and has important developmental value and significance.

[0071] Those skilled in the art should understand that the above description is merely a specific embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A scarless wound healing promoting material, characterized in that: The material is prepared by blending a tissue binder, a bioactive peptide, and a conductive material; the tissue binder is a polyurethane binder; the bioactive peptide is one or more of soybean peptide, acetyl hexapeptide-8, corn peptide, and palmitoyl tripeptide-1; the conductive material is one or more of graphite, nano-silver powder, and gallium-indium alloy; the tissue binder is prepared by solution reaction of raw materials comprising the following components: 100 parts by weight of isocyanate, 300-600 parts by weight of polypolyol mixture, 15-55 parts by weight of chain extender, 2-10 parts by weight of catalyst, and 500-1000 parts by weight of solvent.

2. The scarless wound healing promoting material according to claim 1, characterized in that: The amount of each substance added is calculated based on 100 parts by weight of tissue adhesive, 600-1500 parts by weight of conductive material, and 5-30 parts by weight of biopeptides.

3. The scarless wound healing promoting material according to claim 1, characterized in that, In the scarless wound healing promoting material, the conductive material has conductivity and antibacterial properties, the tissue adhesive has tissue adhesion and helps stop wound bleeding, and the bioactive peptides improve the wettability of metals, enhance interfacial interactions, and have anti-inflammatory effects.

4. The scarless wound healing promoting material according to claim 1, characterized in that, The isocyanate is one or a mixture of two or more of isophorone diisocyanate, dicyclohexylmethane-4,4'-diisocyanate, and diphenylmethane diisocyanate.

5. The scarless wound healing promoting material according to claim 1, characterized in that, The polyol mixture is a mixture of two or more of polyether diols 400-3000 or polyester diols 400-3000; the chain extender is one of dihydrazide carbonate, oxaloyl dihydrazide, heptane dihydrazide, and dodecane dicarboxylic acid dihydrazide; the catalyst is an organotin catalyst; and the solvent is dimethylformamide.

6. The scarless wound healing promoting material according to claim 1, characterized in that, The preparation process of the tissue adhesive is as follows: a mixture of polyols, a catalyst and isocyanate are dissolved in a solvent in proportion, the temperature is raised to 60℃-80℃ and stirred for 2-3 hours; after cooling to 10℃-30℃, a chain extender is added and stirred at room temperature for 2-4 hours; after removing the solvent, it is dissolved in anhydrous ethanol solution for later use.

7. A method for preparing a scarless wound healing promoting material according to any one of claims 1-6, characterized in that, Includes the following steps: The conductive material is dispersed in an alcohol-water solution containing dissolved biopeptides using ultrasound for 10-120 minutes; anhydrous ethanol solution of tissue adhesive is added according to the formula and ultrasound is performed for 1-3 minutes; the dispersion is poured into a mold or scraped into a film, and after drying, a scarless wound healing promoting material is obtained.

8. The application of a scarless wound healing promoting material according to any one of claims 1-6 or a scarless wound healing promoting material obtained by the preparation method of claim 7 in medical materials, characterized in that, The scarless wound healing material is non-toxic, has tissue adhesion and skin compliance, can effectively bond to prevent wounds from reopening, and has hemostatic, anti-leakage, and antibacterial functions.

9. The application of the scarless wound healing promoting material according to claim 8, characterized in that, With the assistance of electrical stimulation, it can activate hair follicle regeneration, reduce scar formation, and can be used in scar removal adhesives that mediate scarless repair as well as other medical materials for scar prevention.

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