A biological painless blood sampling microneedle chip manufacturing device and manufacturing method

Abstract

This invention discloses a biological painless blood collection microneedle chip manufacturing device and method, relating to the field of microneedle chip manufacturing and analysis. It includes: a base; a test frame fixedly connected to the base; a placement ring fixedly connected to the test frame; the placement ring is used to hold the chip body; microneedles are disposed on the chip body; and an analysis plate slidably connected to the test frame. In this invention, through the analysis sleeve set in the device, during the analysis process, the microneedles are inserted into the analysis sleeve by sliding down. After the microneedles are fully inserted into the analysis sleeve, the operator observes whether the light ring on the analysis sleeve lights up to determine if the diameter of the microneedles meets the standard size requirements. By observing whether the lamp on the analysis sleeve lights up, the operator determines whether the height of the microneedles meets the standard size requirements. By observing the brightness of the lamp, the operator analyzes whether the height of the microneedles exceeds the standard requirements.

Description

Technical Field

[0001] This invention belongs to the field of microneedle chip manufacturing and analysis technology, specifically, it relates to a biological painless blood collection microneedle chip manufacturing equipment and manufacturing method. Background Technology

[0002] Human blood sampling is a crucial step in human testing. Currently, blood samples are typically obtained through venipuncture or by pricking the finger with a needle. However, this sampling method can cause pain for the patient. With continuous research and development, researchers have gradually developed a painless blood collection microneedle chip. When using this chip, the blood collection process can be completed simply by placing the microneedle chip on a vein. The pain associated with microneedle chip sampling is significantly reduced.

[0003] Existing microneedle chip fabrication technology is relatively mature. A method for fabricating an ultrafine hollow microneedle chip was disclosed on September 28, 2021, with publication number CN113443603A. This method uses a silicon substrate as the main body and employs semiconductor manufacturing technology to first fabricate an ultrafine needle structure, then fabricate a beveled needle tip, and finally open a channel connecting the needle surface from the back of the silicon substrate. The hollow silicon needle provided by this invention utilizes silicon nitride to accelerate the internal etching reaction during deep silicon etching, resulting in a finer needle. Furthermore, the needle tip and surface angles are etched using anisotropic wet etching of the Si crystal plane, making it easier for the needle to penetrate skin tissue for drug delivery.

[0004] However, the above-mentioned fabrication technology still lacks a step for inspecting the microneedle body on the microneedle chip. If manual inspection is used, due to the small size of the microneedle body, it may not be possible to accurately observe whether the diameter of the microneedle body meets the standard size requirements. At the same time, when analyzing and inspecting the height of the microneedle body, if manual observation is used, it may not be possible to accurately determine whether the height is within the standard size range. The accuracy of the inspection needs to be further improved. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a biological painless blood collection microneedle chip manufacturing device and manufacturing method that can overcome the above problems or at least partially solve the above problems.

[0006] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is: a biological painless blood collection microneedle chip manufacturing device, comprising:

[0007] Base;

[0008] The test fixture is fixedly connected to the base;

[0009] Placement rings, which are fixedly connected to the test fixture;

[0010] The placement ring is used to hold the chip body;

[0011] The chip body is provided with microneedle bodies, and multiple sets of microneedle bodies are evenly distributed on the chip body;

[0012] An analysis plate is slidably connected to the test fixture, and the analysis plate is located directly above the placement ring;

[0013] An analytical sleeve is fixedly connected to an analytical plate. Multiple sets of the analytical sleeves are evenly distributed on the analytical plate, and the analytical sleeves correspond to the microneedle body.

[0014] Preferably, the conductive sheet is fixedly connected in the analytical sleeve;

[0015] The first power supply is fixedly connected to the analytical sleeve;

[0016] The lamp ring is fixedly connected to the analytical sleeve;

[0017] A first wire is fixedly connected to the first power supply. The first power supply, the conductive sheet, the microneedle body, and the lamp ring are connected through the first wire, and the first power supply, the conductive sheet, and the lamp ring are combined to form a complete circuit.

[0018] The rheostat is fixedly connected in the analysis sleeve;

[0019] The slider is slidably connected to the rheostat;

[0020] The second power source is fixedly connected inside the analysis sleeve;

[0021] The second wire is fixedly connected to the second power source;

[0022] The lamp tube is fixedly connected to the analytical sleeve;

[0023] The second power supply, the rheostat, and the lamp are connected by a second wire, and the combination of the second power supply, the rheostat, the microneedle body, and the lamp connected by the second wire forms a complete circuit.

[0024] To improve the stability of the sliding of the analysis plate, a sliding plate is fixedly connected to the analysis plate, and the sliding plate is slidably connected to the test frame.

[0025] In order to quickly fix the analysis plate onto the test frame when the slide plate is reset, a first magnet is fixedly connected to the slide plate and a second magnet is fixedly connected to the test frame, and the first magnet and the second magnet attract each other.

[0026] To prevent the slide from slipping excessively and falling off the test frame, a limiting plate is further fixedly connected to the test frame.

[0027] To improve the stability of the placement ring, a connecting plate is further fixedly connected to the placement ring, and the connecting plate is fixedly connected to the test frame.

[0028] To ensure that the chip body will not fall out of the placement ring when placed in the ring, a limiting piece is further fixedly connected to the bottom of the placement ring.

[0029] To facilitate staff observation of whether the light ring is lit, the light ring emits green light.

[0030] To facilitate staff observation of the lamp's brightness, the lamp emits red light.

[0031] A method for manufacturing a biological painless blood collection microneedle chip manufacturing device based on the above includes the following steps:

[0032] Step 1: Grow silicon dioxide on the front side of the silicon substrate, and deposit silicon nitride on the silicon dioxide;

[0033] Step 2: Form a masking film on silicon nitride using photolithography.

[0034] Step 3: Remove the silicon nitride outside the masking film to expose the silicon dioxide, then remove the exposed silicon dioxide to expose the silicon substrate;

[0035] Step 4: Etch the exposed silicon substrate, etching trenches on the silicon substrate to form a needle structure;

[0036] Step 5: Remove the remaining masking film, silicon nitride and silicon dioxide in sequence, and then deposit a silicon nitride insulating layer on the surface of the silicon substrate.

[0037] Step 6: Remove the silicon nitride barrier layer on the front side of the silicon substrate, while retaining the silicon nitride barrier layer on the back side of the silicon substrate and in the trench.

[0038] Step 7: Etch the angles of the needle tip and needle surface;

[0039] Step 8: Remove the remaining silicon nitride barrier layer;

[0040] Step 9: Apply a needle tip protective layer to the front side of the silicon substrate;

[0041] Step 10: Form an irregularly shaped adhesive film on the back of the silicon substrate to create channels on the needle surface;

[0042] Step 11: Open the needle surface to obtain the needle tube structure;

[0043] Step 12: Remove the irregularly shaped adhesive film and needle tip protective layer to obtain an ultrafine hollow microneedle chip;

[0044] Step 13: The analytical plate in the sliding preparation device is used to test the molded microneedle body by moving the analytical sleeve downward;

[0045] Step 14: When the diameter of the microneedle body is not up to standard, the microneedle body and the conductive plate do not contact when the analytical sleeve is inserted into the microneedle body, and the lamp ring does not light up at this time; when the diameter of the microneedle body is up to standard, the microneedle body and the conductive plate contact when the analytical sleeve is inserted into the microneedle body, and the lamp ring lights up at this time.

[0046] Step 15: After the analysis and testing are passed, the product is released from the warehouse.

[0047] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art: In the present invention, through the analysis sleeve set in the device, during the analysis process, the microneedle body is inserted into the analysis sleeve by sliding down the analysis sleeve. After the microneedle body is fully inserted into the analysis sleeve, the staff can observe whether the lamp ring on the analysis sleeve is lit to know whether the diameter of the microneedle body meets the standard size requirements. By observing whether the lamp tube on the analysis sleeve is lit, it can be determined whether the height of the microneedle body meets the standard size requirements. By observing the brightness of the lamp tube, it can be analyzed whether the height of the microneedle body exceeds the standard requirements. Attached Figure Description

[0048] Figure 1 This is a three-dimensional structural diagram of the painless blood collection microneedle chip manufacturing device proposed in this invention. Figure 1 ;

[0049] Figure 2 This is a three-dimensional structural diagram of the painless blood collection microneedle chip manufacturing device proposed in this invention. Figure 2 ;

[0050] Figure 3 This is a schematic diagram of the placement ring structure in the painless blood collection microneedle chip manufacturing device proposed in this invention;

[0051] Figure 4 This is a schematic diagram of the analytical sleeve in the painless blood collection microneedle chip manufacturing equipment proposed in this invention;

[0052] Figure 5 This is a cross-sectional view of the analytical sleeve in the painless blood collection microneedle chip manufacturing equipment proposed in this invention;

[0053] Figure 6 This is a schematic diagram of the variable resistor in the painless blood collection microneedle chip manufacturing equipment proposed in this invention.

[0054] In the diagram: 1. Base; 101. Test fixture; 1011. Limiting plate; 1012. Second magnet; 102. Display plate; 1021. Display plate; 2. Analysis plate; 201. Slide plate; 2011. First magnet; 3. Placement ring; 301. Limiting piece; 302. Connecting plate; 4. Chip body; 401. Microneedle body; 5. Analysis sleeve; 501. Lamp tube; 502. Second power supply; 6. First wire; 7. First power supply; 701. Lamp ring; 8. Rheostat; 801. Slider; 9. Second wire; 10. Conductive sheet. Detailed Implementation

[0055] The specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0056] Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprises" shall be understood to include the stated elements or components without excluding other elements or other components.

[0057] Example 1: Refer to Figure 1 , Figures 4-6 Painless blood collection microneedle chip manufacturing equipment, including:

[0058] Base 1;

[0059] Test fixture 101 is fixedly connected to base 1;

[0060] Place ring 3 and fix it to test fixture 101;

[0061] Placement ring 3 is used to hold the chip body 4;

[0062] The chip body 4 is provided with microneedle bodies 401, and multiple sets of microneedle bodies 401 are evenly distributed on the chip body 4.

[0063] Analysis plate 2 is slidably connected to test fixture 101, and analysis plate 2 is located directly above placement ring 3;

[0064] The analytical sleeve 5 is fixedly connected to the analytical plate 2. Multiple sets of analytical sleeves 5 are evenly distributed on the analytical plate 2, and the analytical sleeves 5 correspond to the microneedle body 401.

[0065] The conductive sheet 10 is fixedly connected in the analytical sleeve 5;

[0066] The first power supply 7 is fixedly connected to the analysis sleeve 5;

[0067] The lamp ring 701 is fixedly connected to the analysis sleeve 5;

[0068] A first wire 6 is fixedly connected to the first power supply 7. The first power supply 7, the conductive sheet 10 and the lamp ring 701 are connected through the first wire 6, and the first power supply 7, the conductive sheet 10 and the lamp ring 701 and the microneedle body 401 are combined to form a complete circuit.

[0069] The rheostat 8 is fixedly connected in the analysis sleeve 5;

[0070] Sliding contact 801 is slidably connected to rheostat 8;

[0071] The second power supply 502 is fixedly connected in the analysis sleeve 5;

[0072] The second wire 9 is fixedly connected to the second power supply 502;

[0073] Lamp tube 501 is fixedly connected to the analytical sleeve 5;

[0074] The second power supply 502, the rheostat 8, and the lamp tube 501 are connected by the second wire 9, and the second power supply 502, the rheostat 8, the lamp tube 501, and the sliding microneedle body 401 connected by the second wire 9 form a complete circuit.

[0075] Reference Figure 1 A first magnet 2011 is fixedly connected to the slide plate 201, and a second magnet 1012 is fixedly connected to the test frame 101. The first magnet 2011 and the second magnet 1012 attract each other.

[0076] Reference Figure 1 After the test is completed, by pushing the analysis plate 2 upward, the analysis plate 2 slides up a distance, and the first magnet 2011 on the analysis plate 2 will attract the second magnet 1012. At this time, the analysis plate 2 will be fixed on the test frame 101.

[0077] The attraction force of the first magnet 2011 and the second magnet 1012 is greater than the overall weight of the analysis plate 2 and the multiple sets of analysis sleeves 5, thereby ensuring the stability of the analysis plate 2 fixed on the test frame 101.

[0078] Reference Figure 1 , Figure 5The prepared chip body 4 is placed on the placement ring 3. When placing the chip body 4, the microneedle body 401 on the chip body 4 is aligned with the analysis sleeve 5, so that the microneedle body 401 is directly below the analysis sleeve 5, and the highest point of each group of microneedle bodies 401 is directly below the slider 801. After adjustment, the analysis plate 2 is manually pressed down. At this time, the analysis plate 2 receives downward pressure, and the first magnet 2011 on the analysis plate 2 will separate from the second magnet 1012. After separation, the analysis plate 2 can be manually pushed down. The analysis plate 2 will slide down and drive the analysis sleeve 5 fixedly connected to it to slide down. When the analysis plate 2 slides down a certain distance, the microneedle body 401 will extend into the analysis sleeve 5. When the analysis sleeve 5 and the upper surface of the chip body 4 are in contact, it can be observed whether the lamp ring 701 lights up.

[0079] When the diameter of the microneedle body 401 is smaller than the standard diameter, the microneedle body 401 cannot touch the conductive sheet 10. At this time, the first power supply 7, the conductive sheet 10, the lamp ring 701 and the microneedle body 401 cannot be combined to form a complete circuit. At this time, the first power supply 7 cannot supply power to the lamp ring 701 through the first wire 6. At this time, the lamp ring 701 will not light up.

[0080] When the diameter of the microneedle body 401 is equal to the standard diameter, the sidewall of the microneedle body 401 and the conductive sheet 10 are in contact. At this time, the first power supply 7, the conductive sheet 10, the lamp ring 701 and the microneedle body 401 are combined to form a complete circuit. At this time, the first power supply 7 will supply power to the lamp ring 701 through the first wire 6, and the lamp ring 701 will light up.

[0081] When staff analyze and test the diameter of the microneedle body 401, they can determine whether the diameter of the microneedle body 401 meets the qualified standard size by observing whether the light ring 701 set on the sleeve 5 is lit.

[0082] Reference Figure 1 , Figure 5 , Figure 6 During the analysis of the downward movement of sleeve 5,

[0083] If the height of the microneedle body 401 does not meet the standard size requirements, the highest point of the microneedle body 401 will not abut against the slider 801. At this time, the slider 801 is at the lowest point, and the lamp tube 501 will not light up.

[0084] If the height of the microneedle body 401 meets the standard size requirements, when the analysis sleeve 5 moves down, the microneedle body 401 will abut against the slider 801. During the downward movement of the analysis sleeve 5, the microneedle body 401 will push the slider 801 upward. When the analysis sleeve 5 moves down to be in contact with the chip body 4, the slider 801 will slide to the middle of the rheostat 8. At this time, the operating resistance of the rheostat 8 is half of the total resistance of the rheostat 8, and the lamp 501 will light up.

[0085] If the height of the microneedle body 401 is higher than the standard size requirement, during the downward movement of the analysis sleeve 5, the microneedle body 401 will push the slider 801 upward. When the analysis sleeve 5 moves down to be in contact with the chip body 4, the sliding height of the slider 801 is located at the upper end of the rheostat 8 and is higher than the middle position of the rheostat 8. At this time, the operating resistance of the rheostat 8 is greater than half of the total resistance of the rheostat 8. At this time, the brightness of the lamp tube 501 will be greater than the brightness when it is of the standard size.

[0086] The microneedle body 401 is a titanium alloy needle. During the sliding process of the slider 801, the hardness of the microneedle body 401 meets the pushing force of the slider 801, and it will not be deformed, bent or damaged due to pushing the slider 801.

[0087] When inspecting the height of the microneedle body 401, the staff can determine whether the height of the microneedle body 401 meets the standard size requirements by observing whether the lamp tube 501 fixedly connected to the sleeve 5 is lit. By observing the brightness of the lamp tube 501, the staff can determine whether the height of the microneedle body 401 exceeds the standard requirements.

[0088] During the analysis process, the microneedle body 401 is inserted into the analysis sleeve 5 by sliding down the analysis sleeve 5. After the microneedle body 401 is fully inserted into the analysis sleeve 5, the staff can observe whether the lamp ring 701 on the analysis sleeve 5 is lit to determine whether the diameter of the microneedle body 401 meets the standard size requirements. By observing whether the lamp tube 501 on the analysis sleeve 5 is lit, the staff can determine whether the height of the microneedle body 401 meets the standard size requirements. By observing the brightness of the lamp tube 501, the staff can analyze whether the height of the microneedle body 401 exceeds the standard requirements.

[0089] Reference Figure 1 A slide plate 201 is fixedly connected to the analysis plate 2, and the slide plate 201 is slidably connected to the test frame 101.

[0090] A limit plate 1011 is fixedly connected to the test fixture 101.

[0091] By using the slide plate 201 set on the analysis plate 2, the stability of the analysis plate 2 when it slides up and down can be ensured.

[0092] The limiting plate 1011 ensures that the analysis plate 2 will not slip off the test frame 101 due to excessive sliding when it slides upward.

[0093] Reference Figure 3 A connecting plate 302 is fixedly connected to the placement ring 3, and the connecting plate 302 is fixedly connected to the test frame 101.

[0094] By using the connecting plate 302 on the placement ring 3, the stability of the connection between the placement ring 3 and the test fixture 101 can be improved, thereby ensuring the stability of the chip body 4 during analysis and testing.

[0095] Reference Figure 3 The bottom of the ring 3 is fixedly connected to the limiting piece 301.

[0096] The limiting piece 301 set on the placement ring 3 can support the chip body 4, so that the chip body 4 will not fall off the placement ring 3 when it is placed in the placement ring 3.

[0097] Reference Figure 5 The light ring 701 emits green light.

[0098] The light emitted by tube 501 is red.

[0099] By setting the light emission color of the light ring 701 to green and the light emission color of the light tube 501 to red, it is easier for staff to observe the brightness of the light ring 701 and the light tube 501.

[0100] A method for manufacturing a bio-based painless blood collection microneedle chip, including manufacturing equipment, and the specific preparation steps are as follows:

[0101] Step 1: Grow silicon dioxide on the front side of the silicon substrate, and deposit silicon nitride on the silicon dioxide;

[0102] Step 2: A masking film is formed on silicon nitride using a photolithography process to form the needle structure on the silicon substrate;

[0103] Step 3: Use dry etching technology to remove the silicon nitride outside the masking film to expose silicon dioxide, and then use wet etching technology to remove the exposed silicon dioxide to expose the silicon substrate.

[0104] Step 4: Using deep silicon etching technology, anisotropic etching is performed on the exposed silicon substrate to etch trenches on the silicon substrate, forming a needle structure.

[0105] Step 5: Remove the remaining masking film, silicon nitride and silicon dioxide in sequence, and then deposit a silicon nitride insulating layer on the surface of the silicon substrate.

[0106] Step 6: Remove the silicon nitride barrier layer on the front side of the silicon substrate using ICP-RIE reaction, while retaining the silicon nitride barrier layer on the back side of the silicon substrate and in the trench.

[0107] Step 7: Use the anisotropic wet etching method of Si crystal plane to etch the tip and the angle of the needle surface;

[0108] Step 8: Wet removal of the remaining silicon nitride barrier layer;

[0109] Step 9: Apply a needle tip protective layer to the front side of the silicon substrate;

[0110] Step 10: Use photolithography to form an irregularly shaped adhesive film on the back of the silicon substrate to create channels on the needle surface;

[0111] Step 11: Etch deep holes from the back of the silicon substrate to transfer the pattern of the irregularly shaped adhesive film to the needle surface, and open the needle surface to obtain the needle structure.

[0112] Step 12: Remove the irregularly shaped adhesive film and needle tip protective layer to obtain an ultrafine hollow microneedle chip;

[0113] Step 13: The analytical plate 2 in the sliding preparation device is used to test the molded microneedle body 401 by moving the analytical sleeve 5 downward.

[0114] Step Fourteen: For customers who pass the test, the goods are released from the warehouse.

[0115] Example 2: Refer to Figures 1-3 The painless blood collection microneedle chip manufacturing equipment is basically the same as that in Example 1. Furthermore, a display disc 102 is fixedly connected to the base 1, and a display plate 1021 is fixedly connected to the display disc 102. Multiple sets of display plates 1021 are evenly distributed on the display disc 102, and the display plates 1021 and the microneedle body 401 correspond to each other.

[0116] When the chip body 4 is placed on the placement ring 3, the microneedle body 401 on the chip body 4 is located directly above the display plate 1021.

[0117] The display panel 1021 is a white panel.

[0118] During the height analysis and testing of the microneedle body 401, when the height of the microneedle body 401 meets the requirements and the lamp tube 501 is lit, the light from the lamp tube 501 will illuminate the microneedle body 401.

[0119] When the pinhole on the microneedle body 401 is a through hole, the light from the lamp tube 501 will pass through the pinhole and shine on the display plate 1021. At this time, the pinhole on the microneedle body 401 will be a standard through hole.

[0120] When the pinhole on the microneedle body 401 is blocked and is not a through hole, the light from the lamp tube 501 will not pass through the pinhole and illuminate the display plate 1021. At this time, the pinhole on the microneedle body 401 will not pass through the standard, and the microneedle body 401 at this point will not meet the product standard requirements.

[0121] During the height analysis and testing process, this device can also use the illumination light from the lamp tube 501 to illuminate the microneedle body 401, and observe whether the illumination light can pass through the microneedle body 401 and illuminate the display plate 1021. By observing whether there is an illumination spot on the display plate 1021, it can be determined whether the pinhole of the microneedle body 401 at that location is a through hole, and thus quickly determine whether the microneedle body 401 at that location meets the standard.

[0122] Example 3: Reference Figure 3 The painless blood collection microneedle chip manufacturing equipment is basically the same as that in Example 2. The difference is that a light sensor is connected to the display disc 102. The light sensor is a KE3003-25TEMT6000 light sensor. Multiple light sensors correspond to the microneedle body 401, and multiple light sensors are located directly below the microneedle body 401.

[0123] When the light from the lamp tube 501 passes through the microneedle body 401 and shines on the light sensor, the light sensor can receive the light signal and convert it into an electrical signal. At this time, the staff can observe the change in the electrical signal of the light sensor to know whether the light from the lamp tube 501 has passed through the microneedle body 401 and shines on the light sensor, and thus know whether the pinhole of the microneedle body 401 is a through hole.

[0124] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A biological painless blood collection microneedle chip manufacturing device, characterized in that, include: Base (1); The test fixture (101) is fixedly connected to the base (1); Placement ring (3) is fixedly connected to test fixture (101); The placement ring (3) is used to hold the chip body (4); The chip body (4) is provided with microneedle bodies (401), and multiple sets of microneedle bodies (401) are evenly distributed on the chip body (4); The analysis plate (2) is slidably connected to the test frame (101), and the analysis plate (2) is located directly above the placement ring (3); The analysis sleeve (5) is fixedly connected to the analysis plate (2). Multiple sets of the analysis sleeve (5) are evenly distributed on the analysis plate (2), and the analysis sleeve (5) corresponds to the microneedle body (401). Also includes: The conductive sheet (10) is fixedly connected in the analytical sleeve (5); The first power supply (7) is fixedly connected to the analysis sleeve (5); The lamp ring (701) is fixedly connected to the analysis sleeve (5); A first wire (6) is fixedly connected to the first power supply (7). The first power supply (7), the conductive sheet (10) and the lamp ring (701) are connected through the first wire (6). The first power supply (7), the conductive sheet (10), the microneedle body (401) and the lamp ring (701) are combined to form a complete circuit. The rheostat (8) is fixedly connected in the analysis sleeve (5); The slider (801) is slidably connected to the rheostat (8); The second power supply (502) is fixedly connected in the analysis sleeve (5); The second wire (9) is fixedly connected to the second power supply (502); The lamp tube (501) is fixedly connected to the analysis sleeve (5); The second power supply (502), the rheostat (8) and the lamp tube (501) are connected by a second wire (9), and the second power supply (502), the rheostat (8), the microneedle body (401) and the lamp tube (501) connected by the second wire (9) form a complete circuit.

2. The biological painless blood collection microneedle chip manufacturing device according to claim 1, characterized in that, A slide plate (201) is fixedly connected to the analysis plate (2), and the slide plate (201) is slidably connected to the test frame (101).

3. The biological painless blood collection microneedle chip manufacturing device according to claim 2, characterized in that, A first magnet (2011) is fixedly connected to the slide plate (201), and a second magnet (1012) is fixedly connected to the test frame (101). The first magnet (2011) and the second magnet (1012) attract each other.

4. The biological painless blood collection microneedle chip manufacturing device according to claim 3, characterized in that, A limiting plate (1011) is fixedly connected to the test fixture (101).

5. The biological painless blood collection microneedle chip manufacturing device according to claim 1, characterized in that, A connecting plate (302) is fixedly connected to the placement ring (3), and the connecting plate (302) and the test frame (101) are fixedly connected.

6. The biological painless blood collection microneedle chip manufacturing device according to claim 5, characterized in that, The bottom of the placement ring (3) is fixedly connected to a limiting piece (301).

7. The biological painless blood collection microneedle chip manufacturing device according to claim 1, characterized in that, The light ring (701) emits green light.

8. The biological painless blood collection microneedle chip manufacturing device according to claim 1, characterized in that, The light tube (501) emits red light.

9. A method for manufacturing a biological painless blood collection microneedle chip manufacturing device based on any one of claims 1-8, characterized in that, The manufacturing method includes the following steps: Step 1: Grow silicon dioxide on the front side of the silicon substrate, and deposit silicon nitride on the silicon dioxide; Step 2: Form a masking film on silicon nitride using photolithography. Step 3: Remove the silicon nitride outside the masking film to expose the silicon dioxide, then remove the exposed silicon dioxide to expose the silicon substrate; Step 4: Etch the exposed silicon substrate, etching trenches on the silicon substrate to form a needle structure; Step 5: Remove the remaining masking film, silicon nitride and silicon dioxide in sequence, and then deposit a silicon nitride insulating layer on the surface of the silicon substrate. Step 6: Remove the silicon nitride barrier layer on the front side of the silicon substrate, while retaining the silicon nitride barrier layer on the back side of the silicon substrate and in the trench. Step 7: Etch the angles of the needle tip and needle surface; Step 8: Remove the remaining silicon nitride barrier layer; Step 9: Apply a needle tip protective layer to the front side of the silicon substrate; Step 10: Form an irregularly shaped adhesive film on the back of the silicon substrate to create channels on the needle surface; Step 11: Open the needle surface to obtain the needle tube structure; Step 12: Remove the irregularly shaped adhesive film and needle tip protective layer to obtain an ultrafine hollow microneedle chip; Step 13: The analytical plate (2) in the sliding preparation device is used to test the molded microneedle body (401) by moving the analytical sleeve (5) downward. Step 14: When the diameter of the microneedle body (401) is not up to standard, when the analytical sleeve (5) is inserted into the microneedle body (401), the microneedle body (401) and the conductive sheet (10) do not contact each other, and the lamp ring (701) does not light up at this time; when the diameter of the microneedle body (401) is up to standard, when the analytical sleeve (5) is inserted into the microneedle body (401), the microneedle body (401) and the conductive sheet (10) contact each other, and the lamp ring (701) lights up at this time. Step 15: After the analysis and testing are passed, the product is released from the warehouse.

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