A processing method of evodia rutaecarpa seeds and a seedling raising method of evodia rutaecarpa

Abstract

This invention belongs to the field of plant cultivation technology, specifically relating to a method for treating seeds of *Trifolium repens* and a method for raising seedlings of *Trifolium repens*. The treatment method includes (1) screening *Trifolium repens* seeds and refrigerating them; (2) washing the seeds in water at 40-60℃; then soaking the seeds in a gibberellin solution and drying them. This method, through refrigeration, washing the seeds at 40-60℃, and soaking them in a gibberellin solution, can improve the germination rate and the seedling rate in the later seedling stage. After treating *Trifolium repens* seeds using this method, germination can begin in 40 days, reaching its peak at 60 days. When the treated seeds are sown, the average germination rate per pot is 95%. Compared with the conventional seedling raising germination rate (below 15%) and germination time (120 days) of existing technologies, this invention significantly improves the germination rate and shortens the germination time. Furthermore, the treated seeds, when sown, have a higher seedling rate.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, specifically relating to a method for treating seeds of Trifolium repens and a method for raising seedlings of Trifolium repens. Background Technology

[0002] Evodia lepta (Spreng.) Merr., a plant belonging to the Rutaceae family, is a major ingredient in the medicinal materials "999 Cold Relief" series and "Sanjiu Stomach Relief," with annual consumption exceeding tens of thousands of tons. Wild Evodia lepta is mainly distributed in subtropical regions such as Guangdong, Guangxi, Fujian, and Hainan. To ensure the supply of Evodia lepta, large-scale artificial cultivation is necessary to guarantee market availability. Currently, the common method for cultivating Evodia lepta is direct sowing in open fields or by artificial seed broadcasting. This method is outdated, has a low germination rate per acre, and requires a large amount of labor.

[0003] Chinese patent document CN104285538A discloses a method for treating and propagating *Trifolium repens* seeds. The method involves collection, washing, and sand stratification, followed by direct seeding in a seedbed or field. The process includes land preparation, manual sowing, covering with soil, covering with shade netting, watering, and manual seedling removal. This requires multiple people working together, and the germination rate is only about 35%, with a relatively low germination rate. Existing techniques for propagating *Trifolium repens* involve numerous steps and require manual labor, resulting in low efficiency. Furthermore, the seedling rate of this method needs further improvement. Summary of the Invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of low germination rate and seedling emergence rate and cumbersome seedling process in the existing technology of *Trifolium repens* seedling cultivation, and thus provide a method for treating *Trifolium repens* seeds and a method for cultivating *Trifolium repens* seedlings.

[0005] To this end, the present invention provides the following technical solution.

[0006] The first aspect of this invention provides a method for treating trifoliate bitter berries, comprising the following steps:

[0007] (1) Select the seeds of the bitter thorn and refrigerate them;

[0008] (2) Wash the seeds in water at 40-60℃; then soak the seeds in gibberellin solution and air dry.

[0009] The gibberellin solution contains gibberellin at a concentration of 200-600 mg / L.

[0010] Preferably, the soaking time is 24-48 hours.

[0011] The refrigeration temperature is 0-4℃, and the time is no more than 8 months;

[0012] Preferably, the refrigeration time is 3-8 months.

[0013] In step (1), a 10-mesh sieve is used when screening Trifoliate bitter seeds.

[0014] A second aspect of the present invention provides a method for cultivating seedlings of Trifolium repens, comprising the following steps:

[0015] (1) The seeds of Trifoliate bitter orange are treated by the treatment method described in any one of claims 1-4;

[0016] (2) Mechanized potting and sowing are carried out; the pot size is 6cm×9cm (diameter×height), and the sowing amount per pot is 2-4 seeds;

[0017] (3) Seedling management: Monitor the data of each indicator. Water once every 5-8 days before the seeds of Trifolium repens germinate. Apply the first compound fertilizer 3-5 times a month for 1-3 months after the seeds of Trifolium repens germinate. Apply the second compound fertilizer once a month for 4-7 months after the seeds of Trifolium repens germinate. When the seedlings reach a height of 25-35cm, start hardening off the seedlings until they are qualified and ready for transplanting.

[0018] The first compound fertilizer includes a first fertilizer and a rooting agent;

[0019] Preferably, the molar ratio of N, P and K in the first fertilizer is 9:(75-85):50;

[0020] Preferably, based on 1 hectare, the molar content of N in the first fertilizer is 8-10 mol;

[0021] Preferably, the mass ratio of the first fertilizer to the rooting agent is 1:(1-3).

[0022] The molar ratio of N, P, and K in the second compound fertilizer is 9:(15-25):22;

[0023] Preferably, based on 1 hectare, the molar content of N in the second compound fertilizer is 8-10 mol.

[0024] Step (2) includes: feeding, filling pots, seed trays, drilling holes, sowing, covering with soil, and watering.

[0025] Preferably, the acupuncture tray has 21 holes and measures 54cm × 28cm;

[0026] Preferably, during the watering process, the automatic watering time for each row of pots does not exceed 30 seconds, and the spray volume does not exceed 150 ml / m. 2 ;

[0027] Preferably, during the watering process, the automatic watering time for each row of pots is 10 seconds, and the spray volume is 50 ml / m. 2 .

[0028] In step (3), the indicators include temperature, humidity, light intensity and CO2;

[0029] Preferably, the temperature is 15-35°C;

[0030] Preferably, the humidity is 20-80%; when the air humidity is below 40% during the day, the sprinkler system in the greenhouse is automatically turned on, watering the seedbed in a misting state for 30 seconds to keep the seedbed moist, promote seed germination and prevent the germinating seedlings from dying due to prolonged drought; no sprinkler operation is performed at night, and the air humidity at night is maintained at 20-80%.

[0031] Preferably, the light intensity is 0-22000 Lux; when the light intensity is greater than 12000 Lux, the shading net on the greenhouse roof will automatically unfold to reduce the direct light intensity inside the greenhouse and prevent young seedlings from being scorched by strong light. The light intensity must be ensured not to exceed 22000 Lux.

[0032] Preferably, the CO2 concentration is 170-1600 ppm.

[0033] The standards for qualified seedlings are: height 40-60cm, main stem diameter not less than 0.4cm, and lignification not less than 5cm.

[0034] The technical solution of this invention has the following advantages:

[0035] 1. The present invention provides a method for treating *Trifolium repens* seeds, comprising (1) screening *Trifolium repens* seeds and refrigerating them; (2) washing the seeds in water at 40-60℃; then soaking the seeds in gibberellin solution and drying them. This method, through refrigeration, washing the seeds at 40-60℃, and soaking them in gibberellin solution, can improve the germination rate and the seedling rate in the later seedling stage. After treating *Trifolium repens* seeds with the method of the present invention, germination can begin in 40 days, and the number of germinations reaches its peak in 60 days. After sowing the treated seeds, the germination rate can be as high as 95%. Compared with the conventional seedling germination rate (less than 15%) and germination time (120 days) of the prior art, the present invention significantly improves the germination rate and shortens the germination time. After sowing the treated seeds, the seedling rate is high.

[0036] Furthermore, washing seeds in water at 40-60℃ softens the seed coat and improves germination rate; without washing, germination rate will be affected and germination time will be prolonged. Soaking seeds in gibberellin solution can improve germination. Cold storage treatment of seeds can preserve seed viability.

[0037] 2. The seedling raising method for Trifolium repens provided by this invention uses mechanized potting, controls the pot size and sowing amount, and combines intelligent and integrated water and fertilizer management technology for seedling management. This achieves automated, intelligent and precise seedling raising operations, reduces fertilizer application, improves fertilizer utilization, and also increases seed germination rate and seedling qualification rate. This reduces the production cost of Trifolium repens seedling raising, and improves farmland utilization and labor efficiency.

[0038] Furthermore, specific sowing equipment can be customized according to the pot specifications, realizing the entire process of seedling cultivation, including feeding, potting, seedling tray placement, sowing, covering with soil, and watering. The output can reach approximately 80,000 seedlings, which is 9-12 times more efficient than traditional seedling production. Using pots of specific specifications, each pot contains 2-4 seeds, ensuring a high germination rate. Moreover, the process of removing seedlings from the nursery is simple; seedlings can be directly removed from the seedling trays. Each seedling is separated by plastic perforations, preventing root cross-contamination and greatly reducing the problem of decreased seedling quality caused by cross-contamination during the nursery process.

[0039] 3. The seedling cultivation method of Trifolium repens provided by this invention manages seedlings through intelligent control index data and integrated water and fertilizer management technology. By adjusting the proportion of N, P, and K in fertilizer at different stages according to the different micronutrient requirements of seedlings at different production stages, it can accurately apply fertilizer, reduce fertilizer amount, improve fertilizer utilization rate, and also ensure the balanced growth of germinated Trifolium repens seedlings, maintain a consistent production rate, and further ensure the germination rate of the three-stage seedling bank. Detailed Implementation

[0040] The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0041] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0042] The automatic paper pot seeding line equipment in the following embodiments is from Dongguan Enzhuo Intelligent Technology Co., Ltd.

[0043] Example 1

[0044] This embodiment provides a method for treating trifoliate bitter berries, including the following steps:

[0045] (1) The seeds of Trifolium repens are harvested around October each year. After being dried in the shade, cracked, and winnowed to remove impurities, they are graded and screened. The seeds are passed through a 10-mesh pharmacopoeia sieve. The Trifolium repens seeds that do not pass through the sieve are retained as seeds for seedling cultivation. The seeds are then stored in a cold storage at 0-4℃ for 150 days.

[0046] (2) First, use pure dish soap to rub and wash away the oil layer on the surface of the bitter seeds, then wash with 40℃ water, and dry after washing; then soak in 200mg / L gibberellin solution for 48h, and then take it out and let it air dry naturally.

[0047] This embodiment also provides a method for raising seedlings of Trifolium repens, including the following steps:

[0048] (1) Three-forked bitter seeds after the above treatment.

[0049] (2) The automatic paper pot sowing line equipment performs the processes of feeding, potting, tray filling, hole punching, sowing, covering with soil, and watering. This process is completed in May each year. The feeding material is lightweight substrate soil; the paper pots are 6cm × 9cm (diameter × height), with 3 seeds sown per pot; the trays are 21-hole (3 × 7), 54cm × 28cm (length × width); one hole is punched in each paper pot; the filled and sown trays are neatly arranged in the greenhouse, with 4000 trays per acre, approximately 80,000 paper pots, corresponding to 80,000 seedlings. The watering rate is 50ml / m². 2 The automatic watering time for each row of pots is 10 seconds.

[0050] (3) Place the seed trays on the greenhouse seedbed and manage the seedlings using intelligent and integrated water and fertilizer management technology. Monitor and control temperature, humidity, light intensity, and CO2 using the smart Internet of Things. When the air humidity is below 40%, the sprinkler system in the greenhouse will automatically turn on, watering the seedbed in a misting state for 30 seconds to keep the seedbed moist, promote seed germination, and prevent the germinated seedlings from dying due to prolonged drought. When the light intensity is above 12000 Lux, the shading net on the greenhouse roof will automatically unfold to reduce the intensity of direct sunlight inside the greenhouse and prevent the tender seedlings from being scorched by strong light.

[0051] Water the seeds of *Trifolium repens* once a week before they germinate. After germination, apply the first compound fertilizer four times a month (this stage corresponds to August to October each year). The first compound fertilizer consists of a first fertilizer and a rooting agent with a mass ratio of 1:2. The molar ratio of N, P, and K in the first fertilizer is 9:80:50 (the first fertilizer is purchased from Yara International Ltd. - produced in Norway). The amount of N in the first fertilizer is 9 mol per hectare.

[0052] Four to seven months after the seeds of *Trifolium repens* germinate, apply a second compound fertilizer once a month (this stage corresponds to November to February of the following year). The molar ratio of N, P, and K in the second compound fertilizer is 9:20:22 (the second compound fertilizer is purchased from Yara International Ltd.—produced in Norway). Based on a per-hectare basis, the amount of N in the second compound fertilizer is 9 mol. When the seedlings reach a height of 30±5 cm, begin to control water and fertilizer to harden them off until the *Trifolium repens* seedlings are qualified and ready for transplanting. The qualified seedling standards are: height 40-80 cm, main stem diameter not less than 0.4 cm, and lignification not less than 5 cm.

[0053] Example 2

[0054] This embodiment provides a method for treating trifoliate bitter berries, including the following steps:

[0055] (1) The seeds of Trifolium repens are harvested around October each year. After being dried in the shade, cracked, and winnowed to remove impurities, they are graded and screened, passing through a 10-mesh pharmacopoeia sieve. The seeds that do not pass through the sieve are retained. The seeds are then stored in a cold storage at 0-4℃ for 130 days.

[0056] (2) First, use pure dish soap to rub and wash away the oil layer on the surface of the bitter seeds, then wash with 50℃ water, and dry after washing; then soak in 400mg / L gibberellin solution for 48h, and then take it out and let it air dry naturally.

[0057] This embodiment also provides a method for raising seedlings of Trifolium repens, including the following steps:

[0058] (1) Three-forked bitter seeds after the above treatment.

[0059] (2) The automatic paper pot sowing line equipment performs the processes of feeding, potting, seeding, drilling, sowing, covering with soil, and watering. The feeding material is lightweight substrate soil; the paper pots are 6cm × 9cm (diameter × height), with 4 seeds sown per pot; the seeding trays are 21-hole (3 × 7), 54cm × 28cm (length × width); one hole is drilled in each paper pot; the seeding trays are neatly arranged in the greenhouse, with 4000 trays per acre, approximately 80,000 paper pots, corresponding to 80,000 seedlings. The watering rate is 50ml / m². 2 The automatic watering time for each row of pots is 10 seconds.

[0060] (3) Place the seed trays on the greenhouse seedbed and manage the seedlings using intelligent and integrated water and fertilizer management technology. Monitor and control temperature, humidity, light intensity, and CO2 using the smart Internet of Things. When the air humidity is below 40%, the sprinkler system in the greenhouse automatically turns on, watering the seedbed in a misting state for 30 seconds to keep the seedbed moist and promote seed germination. This also prevents the germinated seedlings from dying due to prolonged drought. When the light intensity is above 12000 Lux, the shading net on the greenhouse roof automatically unfolds to reduce the intensity of direct sunlight inside the greenhouse and prevent the tender seedlings from being scorched by strong light.

[0061] Water the seeds of *Trifolium repens* once a week before they germinate. After germination, apply the first compound fertilizer four times a month (this stage corresponds to August to October each year). The first compound fertilizer consists of a first fertilizer and a rooting agent with a mass ratio of 1:2. The molar ratio of N, P, and K in the first fertilizer is 9:80:50 (the first fertilizer is purchased from Yara International Ltd. - produced in Norway). The amount of N in the first fertilizer is 9 mol per hectare.

[0062] Four to seven months after the seeds of *Trifolium repens* germinate, apply a second compound fertilizer once a month (this stage corresponds to November to February of the following year). The molar ratio of N, P, and K in the second compound fertilizer is 9:20:22 (the second compound fertilizer is purchased from Yara International Ltd.—produced in Norway). Based on a per-hectare basis, the amount of N in the second compound fertilizer is 9 mol. When the seedlings reach a height of 30±5 cm, begin to control water and fertilizer to harden them off until the *Trifolium repens* seedlings are qualified and ready for transplanting. The qualified seedling standards are: height 40-80 cm, main stem diameter not less than 0.4 cm, and lignification not less than 5 cm.

[0063] Example 3

[0064] This embodiment provides a method for treating trifoliate bitter berries, including the following steps:

[0065] (1) The seeds of Trifolium repens are harvested around October each year. After being dried in the shade, cracked, and winnowed to remove impurities, they are graded and screened, passing through a 10-mesh pharmacopoeia sieve. The seeds that do not pass through the sieve are retained. The seeds are then stored in a cold storage at 0-4℃ for 100 days.

[0066] (2) First, use pure dish soap to rub and wash away the oil layer on the surface of the bitter seeds, then wash with 60℃ water, and dry after washing; then soak in 600mg / L gibberellin solution for 48h, and then take it out and let it air dry naturally.

[0067] This embodiment also provides a method for raising seedlings of Trifolium repens, including the following steps:

[0068] (1) Three-forked bitter seeds after the above treatment.

[0069] (2) The automatic paper pot sowing line equipment performs the processes of feeding, potting, seeding, drilling, sowing, covering with soil, and watering. The feeding material is lightweight substrate soil; the paper pots are 6cm × 9cm (diameter × height), with 4 seeds sown per pot; the seeding trays are 21-hole (3 × 7), 54cm × 28cm (length × width); one hole is drilled in each paper pot; the seeding trays are neatly arranged in the greenhouse, with 4000 trays per acre, approximately 80,000 paper pots, corresponding to 80,000 seedlings. The watering rate is 50ml / m². 2 The automatic watering time for each row of pots is 10 seconds.

[0070] (3) Place the seed trays on the greenhouse seedbed and manage the seedlings using intelligent and integrated water and fertilizer management technology. Monitor and control temperature, humidity, light intensity, and CO2 using the smart Internet of Things. When the air humidity is below 40%, the sprinkler system in the greenhouse automatically turns on, watering the seedbed in a misting state for 30 seconds to keep the seedbed moist and promote seed germination. This also prevents the germinated seedlings from dying due to prolonged drought. When the light intensity is above 12000 Lux, the shading net on the greenhouse roof automatically unfolds to reduce the intensity of direct sunlight inside the greenhouse and prevent the tender seedlings from being scorched by strong light.

[0071] Water the seeds of *Trifolium repens* once a week before they germinate. After germination, apply the first compound fertilizer four times a month (this stage corresponds to August to October each year). The first compound fertilizer consists of a first fertilizer and a rooting agent with a mass ratio of 1:2. The molar ratio of N, P, and K in the first fertilizer is 9:80:50 (the first fertilizer is purchased from Yara International Ltd. - produced in Norway). The amount of N in the first fertilizer is 9 mol per hectare.

[0072] Four to seven months after the seeds of *Trifolium repens* germinate, apply a second compound fertilizer once a month (this stage corresponds to November to February of the following year). The molar ratio of N, P, and K in the second compound fertilizer is 9:20:22 (the second compound fertilizer is purchased from Yara International Ltd.—produced in Norway). Based on a per-hectare basis, the amount of N in the second compound fertilizer is 9 mol. When the seedlings reach a height of 30±5 cm, begin to control water and fertilizer to harden them off until the *Trifolium repens* seedlings are qualified and ready for transplanting. The qualified seedling standards are: height 40-80 cm, main stem diameter not less than 0.4 cm, and lignification not less than 5 cm.

[0073] Comparative Example 1

[0074] This comparative example provides a method for treating trifoliate bitter berries, including the following steps:

[0075] (1) The seeds of Trifolium repens are harvested around October each year. After being dried in the shade, cracked, and winnowed to remove impurities, they are graded and screened, passing through a 10-mesh pharmacopoeia sieve. The seeds that do not pass through the sieve are retained. The seeds are then stored in a cold storage at 0-4℃ for 200 days.

[0076] (2) First, use pure dish soap to rub and wash away the oil layer on the surface of the bitter seeds, then wash with 68°C water, and dry after washing; then soak in 400mg / L gibberellin solution for 48h, and then take it out and let it air dry naturally.

[0077] This comparative example also provides a method for raising seedlings of Trifolium repens, including the following steps:

[0078] (1) Three-forked bitter seeds after the above treatment.

[0079] (2) The automatic paper pot sowing line equipment performs the processes of feeding, potting, seeding, drilling, sowing, covering with soil, and watering. The feeding material is lightweight substrate soil; the paper pots are 6cm × 9cm (diameter × height), with 3 seeds sown per pot; the seeding trays are 21-hole (3 × 7), 54cm × 28cm (length × width); one hole is drilled in each paper pot; the seeding trays are neatly arranged in the greenhouse, with 4000 trays per acre, approximately 80,000 paper pots, corresponding to 80,000 seedlings. The watering rate is 50ml / m². 2 The automatic watering time for each row of pots is 10 seconds.

[0080] (3) Place the seed trays for sowing on the seedbed of a regular greenhouse and water them once a week before the seeds of Trifolium repens germinate. After the seeds of Trifolium repens germinate, apply the first compound fertilizer 4 times a month (this stage corresponds to August to October each year). The first compound fertilizer includes the first fertilizer and the rooting agent with a mass ratio of 1:2. The molar ratio of N, P and K in the first fertilizer is 9:80:50 (the first fertilizer is purchased from Yara International Ltd. - produced in Norway). Based on the per hectare, the amount of N in the first fertilizer is 9 mol.

[0081] Four to seven months after the seeds of *Trifolium repens* germinate, apply a second compound fertilizer once a month (this stage corresponds to November to February of the following year). The molar ratio of N, P, and K in the second compound fertilizer is 9:20:22 (the second compound fertilizer is purchased from Yara International Ltd.—produced in Norway). Based on a per-hectare basis, the amount of N in the second compound fertilizer is 9 mol. When the seedlings reach a height of 30±5cm, begin to control water and fertilizer, hardening off the seedlings until the *Trifolium repens* seedlings are qualified and ready for transplanting. The qualified seedling standards are: height 40cm, main stem diameter not less than 0.4cm, and lignification not less than 5cm.

[0082] Comparative Example 2

[0083] This comparative example provides a method for treating trifoliate bitter seeds, which differs from Example 1 in that it involves washing with water at 35°C.

[0084] This comparative example also provides a method for raising seedlings of Trifolium repens, the same as in Example 1.

[0085] Comparative Example 3

[0086] This comparative example provides a method for treating seeds of Trifolium repens, using indoleacetic acid instead of the gibberellin solution in Example 2. The concentration of the indoleacetic acid solution is 400 mg / L, and the other steps are the same as in Example 2.

[0087] This comparative example also provides a method for raising seedlings of Trifolium repens, the same as in Example 2.

[0088] Table 1. Quality of Trifoliate Bitter Orange Seedlings in Each Example and Comparative Example Note: Germination rate refers to the ratio of the number of germinating pots to the total number of pots; qualified seedling emergence rate refers to the ratio of the number of seedlings that meet the qualified standard to the total number of pots; Qualified seedlings = Total number × Germination rate × Qualified seedling emergence rate.

[0089] The results in the table show that the germination rate of bitter sesame seeds varies when washed with water at different temperatures. Both excessively high and low temperatures significantly reduce the germination rate of bitter sesame seeds. When the water temperature is between 40-60℃, the germination rate of bitter sesame seeds can be guaranteed, as well as the quality rate of bitter sesame seedlings.

[0090] The results of Comparative Example 3 show that soaking the seeds of Trifolium repens in gibberellin solution can significantly improve the germination rate and the seedling qualification rate of Trifolium repens.

[0091] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A method for treating trifoliate bitter berries, characterized in that, Includes the following steps: (1) Select three-pronged bitter seeds and refrigerate; (2) Wash the seeds in water at 40-60℃; then soak the seeds in gibberellin solution and air dry; the mass concentration of gibberellin in the gibberellin solution is 200-600mg / L; the soaking time is 24-48h.

2. The processing method according to claim 1, characterized in that, The refrigeration temperature is 0-4℃, and the time is no more than 8 months.

3. The processing method according to claim 2, characterized in that, The refrigeration period is 3-8 months.

4. The processing method according to any one of claims 1-3, characterized in that, In step (1), a 10-mesh sieve is used when screening Trifoliate bitter seeds.

5. A method for raising seedlings of *Trifolium repens*, characterized in that, Includes the following steps: (1) The seeds of Trifoliate bitter orange are treated by the treatment method described in any one of claims 1-4; (2) Mechanized potting and sowing are carried out; the pot size is 6cm×9cm (diameter×height), and the sowing amount per pot is 2-4 seeds; (3) Seedling management: Monitor the data of each indicator, water once every 5-8 days before the seeds of Trifolium repens germinate; apply the first compound fertilizer 3-5 times a month within 1-3 months after the seeds of Trifolium repens germinate; apply the second compound fertilizer once a month within 4-7 months after the seeds of Trifolium repens germinate; start hardening off the seedlings when the seedlings reach a height of 25-35cm until the seedlings are qualified and ready for transplanting. The first compound fertilizer includes a first fertilizer and a rooting agent; the molar ratio of N, P and K in the first fertilizer is 9:(75-85):50; The molar ratio of N, P, and K in the second compound fertilizer is 9:(15-25):

22.

6. The seedling raising method according to claim 5, characterized in that, Based on 1 hectare, the molar content of N in the first fertilizer is 8-10 mol.

7. The seedling raising method according to claim 6, characterized in that, The mass ratio of the first fertilizer to the rooting agent is 1:(1-3).

8. The seedling raising method according to claim 7, characterized in that, Based on 1 hectare, the molar content of N in the second compound fertilizer is 8-10 mol.

9. The seedling raising method according to any one of claims 5-8, characterized in that, Step (2) includes the following processes: feeding, filling pots, seed trays, drilling holes, sowing, covering with soil, and watering.

10. The seedling raising method according to claim 9, characterized in that, The acupuncture plate has 21 holes and measures 54cm x 28cm.

11. The seedling raising method according to claim 10, characterized in that, During the watering process, the automatic watering time for each row of pots shall not exceed 30 seconds, and the spray volume shall not exceed 150 ml / m. 2 .

12. The seedling raising method according to claim 11, characterized in that, During the watering process, the automatic watering time for each row of pots is 10 seconds, and the spray volume is 50 ml / m. 2 .

13. The seedling raising method according to claim 12, characterized in that, In step (3), the indicators include temperature, humidity, light intensity and CO2.

14. The seedling raising method according to claim 13, characterized in that, The temperature is 15-35℃.

15. The seedling raising method according to claim 14, characterized in that, The humidity level is 20-80%.

16. The seedling raising method according to claim 15, characterized in that, The light intensity is 0-22000 Lux.

17. The seedling raising method according to claim 16, characterized in that, The CO2 concentration is 170-1600 ppm.

18. The seedling raising method according to claim 17, characterized in that, The standards for qualified seedlings are: height 40-60cm, main stem diameter not less than 0.4cm, and lignification not less than 5cm.

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