Method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum NCPSLZ1

Abstract

The present invention belongs to the field of microbial fermentation technology, and specifically relates to a method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum. The method of the present invention comprises the following steps: S1: crushing Acer truncatum seeds, S2: preparing and activating Ganoderma lucidum seed liquid, S3: preparing culture medium, and S4: fermentation and culturing. Compared with Acer truncatum seed tea that has not been fermented or fermented with commercially available Ganoderma lucidum strains, the Acer truncatum seed tea fermented with the Ganoderma lucidum strain NCPSLZ1 of the present invention has a significantly increased content of flavonoids, a more thorough dissolution of active ingredients, and a full utilization of active ingredients in the Acer truncatum seeds. The resulting tea product has a certain health-care effect, and compared with unfermented Acer truncatum seed tea, the astringency after soaking is significantly improved, making it suitable for daily drinking.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum. Background Art

[0002] Ganoderma lucidum possesses important pharmacological effects, including anti-tumor, immunomodulatory, lipid regulation, blood sugar reduction, hypoxia resistance, and free radical scavenging. These pharmacological activities are closely related to the polysaccharides and triterpenoids contained in Ganoderma lucidum. Testing of the active ingredient content of different strains of Ganoderma lucidum, a type of Ganoderma lucidum resembling deer antlers, revealed that the crude polysaccharide content of Ganoderma lucidum from Deer Antler is significantly higher than that of other Ganoderma lucidum strains, suggesting that Deer Antler Ganoderma lucidum has a higher value for utilization than standard Ganoderma lucidum.

[0003] Acer truncatum seeds are the kernels of Acer truncatum, which are rich in flavonoids, unsaturated fatty acids, vitamin E, protein, trace elements, etc. that are essential to the human body. They have a good effect in regulating brain nerve development and metabolism, scavenging free radicals, preventing cancer, and regulating skin metabolism; the functional fatty acid it contains, neuraminic acid, can also repair and clear damaged brain nerve fibers and promote nerve cell regeneration. It is an essential "advanced nutrient" for the redevelopment and maintenance of nerve cells such as brain cells and optic nerve cells.

[0004] Currently, the primary method for utilizing Acer truncatum seeds is oil extraction. For example, patent application number 201910342215.4 discloses a method for preparing Acer truncatum seed oil, which includes preparation, cleaning and drying, peeling, microwave heating, pressing, filtering, refining, alkali refining, and bleaching and deodorizing to obtain Acer truncatum seed oil. This method utilizes Acer truncatum seeds to produce Acer truncatum seed oil, but the process is complex and costly. Furthermore, repeated microwave heating and pressing damage nutrients such as the oil, preventing the effective utilization of the active substances in the seeds.

[0005] Other documents also describe teas prepared from young or fresh leaves of Acer truncatum. For example, Patent Document No. 200310110788.3 discloses a health-care Acer truncatum tea and its preparation method. The tea uses young or fresh leaves of Acer truncatum as raw material, which are washed, air-dried, and then placed in a steamer for withering. After withering, rolling, shaping, and drying, the tea is produced, which can be consumed as a daily beverage. However, this method uses Acer truncatum leaves as raw material, not Acer truncatum seeds, so the beneficial ingredients of Acer truncatum seeds cannot be utilized. Furthermore, the Acer truncatum leaves are prepared using only conventional tea-making processes. Therefore, when the tea is steeped in boiling water for daily consumption, the beneficial ingredients, such as flavonoids, are difficult to dissolve effectively.

[0006] The inventors have discovered that there are few publications on the use of Acer truncatum seeds in tea products. Furthermore, when Acer truncatum seeds are used in tea products, the active ingredients are often difficult to dissolve simply by soaking in boiling water.

[0007] Therefore, it is of great significance to provide a tea product that can dissolve the effective ingredients in the Acer truncatum seeds more thoroughly, has a higher nutritional value, and thus has a health-care effect on the human body. Summary of the Invention

[0008] In order to solve the above technical problems, the present invention provides a method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum strains. By fermenting Acer truncatum seeds with preserved specific Ganoderma lucidum, the flavonoid components in the Acer truncatum seeds can be dissolved more fully and the astringency can be reduced. People can drink the tea in the form of tea on a daily basis, thereby improving the utilization rate of the Acer truncatum seeds. In addition, the crude polysaccharides and triterpenoid compounds contained in the specific Ganoderma lucidum have anti-tumor, anti-inflammatory and lipid-lowering effects, and can achieve better health care effects when combined with the active ingredients in the Acer truncatum seeds.

[0009] The method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum strains provided by the present invention comprises the following steps:

[0010] S1: crushing the dried Acer truncatum seeds;

[0011] S2: Screening a Ganoderma lucidum strain, preparing a Ganoderma lucidum seed solution, and activating the strain; the screened Ganoderma lucidum strain was deposited with the China Center for Type Culture Collection on December 29, 2015, at Wuhan University, Wuhan, China, with a deposit number of CCTCCNO: M 2015796 and a taxonomic name of Ganoderma amboinense NCPSLZ1;

[0012] S3: mixing the crushed Acer truncatum seeds in S1 with nutrients, sterilizing the mixture, and cooling the mixture to room temperature to obtain a culture medium;

[0013] S4: Fermentation and culture: The Ganoderma lucidum activated in S2 is inoculated into the culture medium in S3, fermented and cultured, and dried to obtain Acer truncatum seed tea.

[0014] Specifically, in S2, the preparation method of the Ganoderma lucidum seed liquid is as follows:

[0015] The potato glucose water culture medium was divided into 500 mL Erlenmeyer flasks, 250 mL per bottle, sterilized at 121 ° C for 30 min, and then taken out after cooling as seed liquid culture medium for later use;

[0016] Cut the activated Ganoderma lucidum into pieces with a cross-sectional area of ​​about 1 cm 2The slant mycelia of different sizes were inoculated into the seed liquid culture medium and cultured in a shaking incubator at 27±2℃ and 150r / min for 7 days.

[0017] Preferably, in S3, the nutrient is at least one of glucose, soluble starch, peptone, and bran.

[0018] Preferably, in S3, when preparing the culture medium, 40-60 mL of water, 0.5-1 wt% of glucose, 0.5-1 wt% of soluble starch, 0.5-1 wt% of peptone, and 0.5-1 wt% of bran are added to every 100 g of Acer truncatum seeds, and the mixture is placed in a culture bag and sterilized at 121° C. for 30 min and then cooled to room temperature to obtain the culture medium.

[0019] Preferably, in S4, 8-12 mL of Ganoderma lucidum seed solution is inoculated into the culture medium per 100 g of sterilized Acer truncatum seeds, and the inoculated culture bag is cultured at 28-30° C. for 12-14 days.

[0020] Preferably, in S4, after the cultivation is completed, the tea is dried to a moisture content of 8-12 wt%, and the dried Acer truncatum seed tea is bagged to make tea bags;

[0021] Alternatively, the dried Acer truncatum seed tea is pressed into any one of a brick shape, a cake shape, and a lump shape to form Acer truncatum seed tea.

[0022] The present invention adopts a specific preserved deer horn Ganoderma lucidum, which is rich in protease, cellulase and ligninase. Utilizing this characteristic, the strain is used as the main biotransformation body, and solid-state fermentation is carried out with Acer truncatum seeds as a matrix. Not only can the fermentation product obtain mycelium components rich in active substances such as Ganoderma polysaccharides, but the nutritional properties of the matrix can also be improved through the biotransformation of Ganoderma lucidum. Compared with ordinary commercially available Ganoderma lucidum strains, the active ingredients in Acer truncatum seeds fermented by this strain can be dissolved more thoroughly.

[0023] Acer truncatum seeds are rich in oil and protein, as well as a certain amount of cellulose, making them a suitable substrate for Ganoderma lucidum fermentation. The components of Acer truncatum seeds, such as protein, free amino acids, and trace elements, promote the growth and metabolism of Ganoderma lucidum and the production of antioxidant substances (such as polysaccharides and triterpenes). Furthermore, during their growth and metabolism, Ganoderma lucidum, leveraging the penetrating power of its hyphae and the enzymatic activity of its hydrolases and oxidases, can depolymerize the cellular structure of the Acer truncatum seed matrix, promoting the release of antioxidant substances. Furthermore, Ganoderma lucidum not only metabolizes substances with antioxidant activity itself but also may convert certain components of the Acer truncatum seed matrix to produce antioxidant substances not originally present in the matrix, such as flavonoids. Ganoderma lucidum fermentation on Acer truncatum seeds is bidirectional, and the resulting mycelium possesses enhanced antioxidant activity.

[0024] In addition, the tea drink prepared by the method of the present invention has the following characteristics:

[0025] The present invention uses Acer truncatum seeds as raw materials and uses specific Ganoderma lucidum to ferment the Acer truncatum seeds, thereby overcoming the disadvantage of the Acer truncatum seeds themselves mentioned in the background art that the active ingredients are difficult to dissolve, effectively improving the dissolution rate of antioxidant active ingredients such as flavonoids and their antioxidant activity; and through the fermentation process, the bitter taste of the Acer truncatum seeds themselves is reduced, making the taste more pleasant to consumers.

[0026] The beneficial effects of the present invention are:

[0027] (1) The product obtained by the method of the present invention has a maximum flavonoid content of about 3.8 mg / g, a total antioxidant capacity of about 21.82 μmol / g, a DPPH scavenging rate of about 45.74%, and an ABTS scavenging rate of about 84.62%. Compared with unfermented Acer truncatum seed tea, the Acer truncatum seed tea prepared by the method provided by the present invention effectively dissolves the active ingredients in the Acer truncatum seeds, thereby improving the nutritional value of the tea product. The total antioxidant capacity is increased by 7.4%, and the DPPH free radical scavenging capacity and ABTS free radical scavenging capacity are increased by 10.0% and 21.8%, respectively.

[0028] However, the flavonoid content of the Acer truncatum seed tea fermented with ordinary commercially available Ganoderma lucidum is significantly lower than that of the present invention, and the DPPH clearance rate and ABTS are also much lower than those of the present invention.

[0029] (2) In terms of taste improvement, the Acer truncatum seed tea of ​​the present invention is sweet and refreshing when taken in the mouth, without obvious astringency, thus overcoming the disadvantage of the unfermented Acer truncatum seed tea having a bitter taste.

[0030] (3) The Acer truncatum seed tea of ​​the present invention not only contains the active substances of the Acer truncatum seeds themselves, but also contains crude polysaccharides and triterpenoid compounds of Ganoderma lucidum, which have anti-tumor, anti-inflammatory and lipid-lowering effects. When combined with the active ingredients in the Acer truncatum seeds, better health care effects can be achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a species map of the strain NCPSLZ1 of the present invention;

[0032] Figure A shows the slant morphology of the strain, and Figure B shows the colony morphology after 7-8 days of culture.

[0033] Figure 2 The invention uses the strain NCPSLZ1 to ferment and prepare Acer truncatum seed tea;

[0034] Figure 2In the figure, A and B are pictures of the cultured cells after 12-14 days; C and D are pictures before and after drying, respectively;

[0035] Figure 3 These are pictures of the finished product of Acer truncatum seed tea fermented with the strain NCPSLZ1 of the present invention, including tea bags (left) and tea soup (right). DETAILED DESCRIPTION

[0036] In order to enable those skilled in the art to better understand the present invention, the present invention will be further explained in conjunction with specific embodiments.

[0037] Example 1

[0038] The method for preparing Acer truncatum seed tea bag by fermenting Ganoderma lucidum comprises the following steps:

[0039] S1: crushing the dried Acer truncatum seeds into 40 mesh;

[0040] S2: Screening Ganoderma lucidum strains, preparing Ganoderma lucidum seed solution, and activating the strains; the deposit number of the Ganoderma lucidum strain is CCTCC NO: M 2015796;

[0041] Unless otherwise specified in this application, all references refer to the Ganoderma lucidum strains with the above-mentioned deposit numbers;

[0042] Specifically, the preparation method of Ganoderma lucidum seed liquid is as follows:

[0043] The potato glucose water culture medium was divided into 500 mL Erlenmeyer flasks, 250 mL per bottle, sterilized at 121 ° C for 30 min, and then taken out after cooling as seed liquid culture medium for later use;

[0044] Cut the activated Ganoderma lucidum into 1cm pieces 2 The slant mycelium of different sizes was inoculated into the seed liquid culture medium and cultured in a shaking incubator at 27°C and 150 r / min for 7 days; Figure 1 shown.

[0045] S3: The crushed Acer truncatum seeds in S1 are mixed with nutrients, sterilized, and cooled to room temperature to obtain a culture medium; when preparing the culture medium, 50 mL of water, 1 wt% of glucose, 1 wt% of soluble starch, 1 wt% of peptone, and 1 wt% of bran are added to every 100 g of Acer truncatum seeds, and the mixture is placed in a culture bag and sterilized at 121° C. for 30 min, and then cooled to room temperature to obtain the culture medium;

[0046] S4: Fermentation culture: Inoculate the culture medium with 10 mL of Ganoderma lucidum seed solution per 100 g of sterilized Acer truncatum seeds. Incubate the inoculated culture bag at 28°C for 13 days. The fermentation culture process is shown in the attached picture. Figure 2After the culture is completed, the mixture is dried to a moisture content of 10 wt% (see attached Figure 2 The dried Acer truncatum seed tea is bagged to make tea bags.

[0047] Example 2

[0048] The method for preparing Acer truncatum seed tea bag by fermenting Ganoderma lucidum comprises the following steps:

[0049] S1, S2, and S4 are the same as in Example 1;

[0050] S3: The crushed Acer truncatum seeds in S1 are mixed with nutrients, sterilized, and cooled to room temperature to obtain a culture medium; when preparing the culture medium, 50 mL of water, 0.5 wt% of glucose, 0.5 wt% of soluble starch, 0.5 wt% of peptone, and 0.5 wt% of bran are added to every 100 grams of Acer truncatum seeds, and the mixture is placed in a culture bag and sterilized. After sterilization at 121° C. for 30 minutes, the mixture is cooled to room temperature to obtain the culture medium.

[0051] Example 3

[0052] The method for preparing Acer truncatum seed tea bag by fermenting Ganoderma lucidum comprises the following steps:

[0053] S1, S2, and S4 are the same as in Example 1;

[0054] S3: The crushed Acer truncatum seeds in S1 are mixed with nutrients, sterilized, and cooled to room temperature to obtain a culture medium; when preparing the culture medium, 50 mL of water, 0.8% glucose, 0.8% soluble starch, 0.8% peptone, and 0.8% bran are added to every 100 grams of Acer truncatum seeds, and the mixture is placed in a culture bag and sterilized. After sterilization at 121° C. for 30 minutes, the mixture is cooled to room temperature to obtain the culture medium.

[0055] Example 4

[0056] The method for preparing Acer truncatum seed tea bag by fermenting Ganoderma lucidum comprises the following steps:

[0057] S1, S2, and S3 are the same as in Example 1;

[0058] S4: Fermentation culture: 8 mL of Ganoderma lucidum seed liquid is inoculated into the culture medium at a ratio of 100 g of sterilized Acer truncatum seeds, and the inoculated culture bag is cultured at 28° C. for 10 days. After the culture is completed, the culture bag is dried to a moisture content of 10 wt %. The dried Acer truncatum seed tea is bagged to make tea bags.

[0059] Example 5

[0060] The method for preparing Acer truncatum seed tea bag by fermenting Ganoderma lucidum comprises the following steps:

[0061] S1, S2, and S3 are the same as in Example 1;

[0062] S4: Fermentation culture: Inoculate the culture medium with 12 mL of Ganoderma lucidum seed solution per 100 g of sterilized Acer truncatum seeds, incubate the inoculated culture bag at 28°C for 13 days, and dry the bag to a moisture content of 10 wt%. Pack the dried Acer truncatum seed tea into tea bags. Figure 3 As shown in the left picture, this picture is a sample of tea bag; the right picture shows the appearance of the tea bag after brewing.

[0063] Example 6

[0064] The method for preparing Acer truncatum seed tea bag by fermenting Ganoderma lucidum comprises the following steps:

[0065] S1, S2, and S3 are the same as in Example 1;

[0066] S4: Fermentation culture: 10 mL of Ganoderma lucidum seed liquid is inoculated into the culture medium at a ratio of 100 g of sterilized Acer truncatum seeds, and the inoculated culture bag is cultured at 29° C. for 13 days. After the culture is completed, the culture bag is dried to a moisture content of 10 wt %. The dried Acer truncatum seed tea is bagged to make tea bags.

[0067] Comparative Example 1A

[0068] Compared with Example 1, in Comparative Example 1, the Acer truncatum seeds were not fermented, but directly baked to be used as tea powder.

[0069] S1: crushing the dried Acer truncatum seeds into 40 mesh;

[0070] S2: Pack the Acer truncatum seeds in S1 to obtain Acer truncatum seed tea.

[0071] Comparative Example 1B

[0072] S1: same as Comparative Example 1A;

[0073] S2: Inoculate freeze-dried Ganoderma lucidum with 10 mL of Ganoderma lucidum seed solution per 100 grams of sterilized Acer truncatum seeds;

[0074] S3: The Acer truncatum seeds obtained in S1 and the freeze-dried bacteria obtained in S2 are fully mixed according to the proportions in Example 1.

[0075] Comparative Example 2

[0076] The difference from Example 1 is that the commercially available Ganoderma lucidum strain 1 is used to prepare the Ganoderma lucidum seed liquid.

[0077] Comparative Example 3

[0078] The difference from Example 1 is that the commercially available Ganoderma lucidum strain 2 is used to prepare the Ganoderma lucidum seed liquid.

[0079] Example 7

[0080] Regarding the Acer truncatum seed tea in the present application, the applicant has determined the flavonoids, total antioxidant capacity, DPPH and ABTS of the tea samples in Examples 1-6 and Comparative Examples 1-3.

[0081] The determination of flavonoids, total antioxidant capacity, DPPH free radical scavenging capacity, and ABTS free radical scavenging capacity were performed using the Solebao flavonoid content, total antioxidant capacity (T-AOC), DPPH free radical scavenging capacity, and ABTS free radical scavenging capacity detection kits, respectively; the detection methods were carried out according to the kit instructions.

[0082] The measurement results are shown in Tables 1 and 2;

[0083] Table 1 Determination of indexes of Acer truncatum seed tea under different conditions

[0084]

[0085] As can be seen from the table above, the antioxidant activity data obtained from the tea products in Examples 1-6 are comparable, demonstrating the stability and reliability of the Acer truncatum seed tea prepared using the method of the present invention. Furthermore, the product in Example 1 showed a flavonoid content of 3.806 mg / g; a DPPH of 45.74, an increase of approximately 3.5 percentage points from the pre-fermentation 41.57%; and an ABTS of 84.62%, an increase of approximately 15 percentage points from the pre-fermentation 69.44%. These data demonstrate that fermenting Acer truncatum seeds with Ganoderma lucidum facilitates the dissolution and increase of the antioxidant activity present in the seeds.

[0086] This is because maple seeds are rich in oil and protein, as well as a certain amount of cellulose, which can serve as a substrate for Ganoderma lucidum fermentation. Components of maple seeds, such as protein, free amino acids, and trace elements, can promote the growth and metabolism of Ganoderma lucidum and the production of antioxidant substances (such as polysaccharides and triterpenes). Furthermore, during its growth and metabolism, Ganoderma lucidum, leveraging the penetrating power of its hyphae and the enzymatic activity of its hydrolases and oxidases, can depolymerize the cellular structure of the maple seed matrix and promote the release of antioxidant substances. Furthermore, Ganoderma lucidum not only metabolizes and produces substances with antioxidant activity itself, but may also transform certain components of the maple seed matrix to produce antioxidant substances not originally present in the matrix, such as flavonoids. Ganoderma lucidum fermentation on the maple seed matrix is ​​bidirectional, and the resulting mycelium possesses higher antioxidant activity.

[0087] Table 2 Determination of indices of Acer truncatum seed tea fermented with different Ganoderma lucidum fungi

[0088] Example 1 Comparative Example 2 Comparative Example 3 Flavonoids (mg / g) 3.806 2.663 3.074 Total antioxidant capacity (umol / g) 21.82 18.51 19.33 DPPH (%) 45.74 39.26 41.83 ABTS (%) 84.62 77.69 79.20

[0089] From the data in Table 2, it can be seen that compared with the Acer truncatum seed tea fermented with ordinary commercially available Ganoderma lucidum, the flavonoids in the product of the present invention are about 1 percentage point higher than the commercial product, the DPPH clearance rate is about 4-6 percentage points higher than the commercial product, and the ABTS clearance rate is about 5-7 percentage points higher than the commercial product. That is, the Acer truncatum seed tea fermented with the Ganoderma lucidum strain NCPSLZ1 of the present invention has a significantly increased flavonoid content and has more excellent antioxidant capacity, as well as DPPH free radical scavenging capacity and ABTS free radical scavenging capacity. It can be seen that although they all belong to the same Ganoderma lucidum strain, the antioxidant activity of the products obtained by fermenting Acer truncatum seeds with different Ganoderma lucidum strains is significantly different. The fermentation of Acer truncatum seed tea with the Ganoderma lucidum strain of the present invention has a more excellent effect.

[0090] Example 8

[0091] Ten tasters were selected to conduct sensory evaluation on the tea samples obtained from each embodiment and comparative example, and sensory scores were given based on the color, smell and taste of the tea samples after soaking. The scoring criteria and scores are as follows:

[0092] Table 3 Sensory scoring criteria for tea samples

[0093]

[0094] The scores for each tea sample are as follows:

[0095] Table 4 Sensory scores of tea samples

[0096] Case Color odor Taste Total score Example 1 23 34 38 95 Example 2 23 34 36 93 Example 3 24 33 38 95 Example 4 22 34 37 93 Example 5 24 34 35 93 Example 6 23 34 35 92 Comparative Example 1A 21 12 18 51 Comparative Example 1B 22 30 33 85 Comparative Example 2 22 33 34 89 Comparative Example 3 23 34 36 93

[0097] As can be seen from the data in Table 4, Examples 1-6 and Comparative Example 3 had relatively high sensory scores, all exceeding 90 points, while Comparative Example 1B and Comparative Example 2 scored below 90, and Comparative Example 1A scored only 51 points, indicating that tea products prepared by fermenting Acer truncatum seeds with Ganoderma lucidum can effectively improve the astringency of Acer truncatum seeds when soaked alone. As can be seen from the data in Comparative Examples 2-3, commercially available Acer truncatum seed tea fermented with Ganoderma lucidum can also improve the astringency of Acer truncatum seeds, but the tea products prepared by fermenting Acer truncatum seeds with the specific Ganoderma lucidum fungus NCPSLZ1 of the present invention have higher and more stable overall sensory scores.

[0098] In summary, the tea product prepared by fermenting Acer truncatum seeds with the specific Ganoderma lucidum NCPSLZ1 of the present invention is richer in nutrients, has a relatively high sensory score, and has a good taste.

Claims

1. A method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum NCPSLZ1, comprising the following steps: S1: crushing the dried Acer truncatum seeds; S2: Screening Ganoderma lucidum strains, preparing Ganoderma lucidum seed liquid, and activating the strains; the screened Ganoderma lucidum was deposited in the China Center for Type Culture Collection on December 29, 2015, at Wuhan University, Wuhan, China, with a deposit number of CCTCC NO: M 2015796 and a classification name of Ganoderma lucidum. Ganoderma amboinense NCPSLZ1; S3: The crushed Acer truncatum seeds in S1 are mixed with nutrients, sterilized, and cooled to room temperature to obtain a culture medium. The specific operation is as follows: 40-60 mL of water, 0.5-1 wt% of glucose, 0.5-1 wt% of soluble starch, 0.5-1 wt% of peptone, and 0.5-1 wt% of bran are added to every 100 g of Acer truncatum seeds, and the mixture is placed in a culture bag and sterilized at 121° C. for 30 min, and then cooled to room temperature to obtain a culture medium. S4: Fermentation culture: inoculate into the culture medium, ferment and culture, inoculate the Ganoderma lucidum activated in S2 into the culture medium of S3 at a ratio of 8-12 mL of Ganoderma lucidum seed liquid per 100 grams of sterilized Acer truncatum seeds, culture the inoculated culture bag at 28-30°C for 12-14 days, and dry to obtain Acer truncatum seed tea.

2. The method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum NCPSLZ1 according to claim 1, characterized in that: In S2, the preparation method of the Ganoderma lucidum seed liquid is as follows: The potato glucose water culture medium was divided into 500 mL Erlenmeyer flasks, 250 mL per bottle, sterilized at 121 ° C for 30 min, and then taken out after cooling as seed liquid culture medium for later use; Cut the activated Ganoderma lucidum into pieces with a cross-sectional area of ​​1 cm 2 The slant mycelia of different sizes were inoculated into the seed liquid culture medium and cultured in a shaking incubator at 27±2℃ and 150r / min for 7 days.

3. The method for preparing Acer truncatum seed tea by fermenting Ganoderma lucidum NCPSLZ1 according to claim 1, characterized in that: In S4, after the incubation is completed, the tea is dried to a moisture content of 8-12 wt%, and the dried Acer truncatum seed tea is bagged to make tea bags; Alternatively, the dried Acer truncatum seed tea is pressed into any one of a brick shape, a cake shape, and a lump shape to obtain Acer truncatum seed tea.

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