Pesticidal compositions and use thereof

Abstract

The present application relates to a kind of insecticidal composition and its application, it is related to the field of pesticide, the effective component of insecticidal composition includes carbamate compound and amide insecticide, and its weight ratio is (1-50) :(1-50);The carbamate compound is selected from one or several of thiodicarb, fenobucarb, fenoxycarb and propetamphos;The amide insecticide is selected from one or several of broflan, cyhalofop-butyl, chlorantraniliprole;Carbamate compound of the present application can be used as the internal absorption of amide insecticide Conducting agent, greatly improve the absorption and transmission amount of amide insecticide in crop, improve the insecticidal effect of amide insecticide, improve the utilization rate of pesticide, reduce environmental pollution.

Description

Technical Field

[0001] This invention relates to the field of pesticides, and in particular to an insecticidal composition and its application. Background Technology

[0002] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art.

[0003] Based on their research on plant-derived pesticides, Xu Hanhong et al. proposed the concept of targeted pesticides: using targeted carriers to deliver the active ingredients of pesticides to the target sites where pests cause damage. (Xu Hanhong, 2009 Research and Application Progress of Plant-Derived Pesticides in China)

[0004] The targeted absorption and translocation properties of pesticides in plants help improve pesticide utilization and reduce dosage levels in non-target areas and the environment. However, this requires the pesticide molecule to couple with a targeting group to increase the compound's transport properties within the plant and enhance the absorption and distribution of the agent in the target area, but it also reduces the biological activity of the original drug. Furthermore, altering the structure of the original drug results in enormous costs for developing new drugs (CN111567541A).

[0005] Therefore, there is still a need to develop targeted pesticides that can be directed without coupling. By leveraging the targeting and systemic properties of these pesticides, the systemic absorption of pesticides can be increased, leading to greater accumulation within the crop and minimizing the amount of pesticide agent or active ingredient used, thus providing optimal control of pests.

[0006] Structure of Fenobucarb

[0007]

[0008] Molecular formula C 11 H 15 NO2 is a carbamate compound with strong systemic activity within crops. Simultaneously, isoprocarb inhibits the activity of cholinesterase in pests, blocking normal nerve conduction and leading to dysregulation of physiological and biochemical processes, resulting in poisoning and death. It has good rapid-acting properties, a short residual period, and strong selectivity, showing good control effects against planthoppers, leafhoppers, and aphids. Isoprocarb is produced by the reaction of methyl isocyanate with o-isopropylphenol.

[0009] The structural formula of Fenobucarb is C 12 H 17 NO2, the structural formula is as follows:

[0010]

[0011] Butylcarbamate is a carbamate insecticide with strong contact action, and also has stomach poison, fumigation, and ovicidal effects. It is fast-acting, but has a short residual period. It is mainly used to control rice planthoppers, rice leafhoppers, and rice thrips. Spray with a 1000-2000 times dilution of 50% Chemicalbook emulsifiable concentrate, or dust with 300-375 g / 100 m² of 2% basil powder. It also has good control effects on rice leaf rollers, stink bugs, rice stem borers, and aphids.

[0012] Fenoxycarb (molecular formula C) 17 H 19 NO4, the structural formula is as follows:

[0013]

[0014] Phenoxycarb is a non-terpenoid insect growth regulator insecticide with juvenile hormone activity. It has a broad insecticidal spectrum, exhibiting both stomach poison and contact action, effectively controlling resistant pests, and is safe for natural enemies. Its insecticidal action is non-neurogenic, showing strong juvenile hormone activity against most insects, preventing egg hatching, inhibiting adult metamorphosis and molting in larvae, causing death in late larval or pupal stages. It also inhibits the growth of adults and larvae. It can be used against fruit tree leafrollers, cotton leaf moths and pyralids, pear psyllids, citrus scale insects, as well as storage pests and red imported fire ants.

[0015] Thiodicarb has the molecular formula C 10 H 18 N404S3, structural formula as follows:

[0016]

[0017] Thiamethoxam is a sulfur-linked dicarboxylic acid ester insecticide with insecticidal activity similar to methomyl, but lower toxicity. Its main effect is stomach poisoning; it has almost no contact action, no fumigation or systemic action, and exhibits strong selectivity with a very short residual period in soil. It is effective against Lepidoptera, Coleoptera, and Diptera pests, and also shows high activity against Lepidoptera eggs. For the control of bollworms and pink bollworms, a 75% suspension at 6–12 mL / 100 m² diluted with water and sprayed evenly can achieve significant insecticidal effects.

[0018] Cyantraniliprole, with the molecular formula C 19 H 14 BrClN6O2, structural formula as follows

[0019]

[0020] Bromnipotent is a systemic insecticide with both stomach poison and contact action, effective against both chewing and piercing-sucking pests (Xu Hongfei, 2022, Functional Study of Chemosensory Proteins in Bromnipotent Resistance in Cotton Aphids). Its target is the insect's RyR, causing uncontrolled release and consumption of calcium ions, inhibiting muscle contraction, and ultimately leading to muscle paralysis and death (Cordova et al., 2006; Jeanguenat, 2013; Sattelle et al., 2008).

[0021] Chlorantraniliprole, with the molecular formula C 18 H 14 BrC l2 N5O2, structural formula as follows:

[0022]

[0023] Chlorantraniliprole is a novel systemic insecticide belonging to the o-formamide class. It possesses a broad insecticidal spectrum, can be used on various crops, and exhibits good residual effect, high activity, low toxicity, and environmental and biosafety. It primarily works by binding to ryanodine receptors in the muscle cells of insect pests, causing abnormal opening of the receptor channels. This results in the unrestricted release of calcium ions from the insect's calcium stores into the cytoplasm, leading to paralysis and death. It exhibits high activity against lepidopteran pests.

[0024] Cyclaniliprole, with the molecular formula C 21 H 17 Br2C l2 N5O2, structural formula as follows:

[0025]

[0026] Cyclobromamide belongs to the amide class of insecticides and has a similar chemical structure and insecticidal mechanism to chlorantraniliprole. However, it does not act on ryanodine receptors, but rather on ryanodine receptor allosteres. This active ingredient has a broad insecticidal spectrum and currently exhibits high insecticidal activity against Lepidoptera, Coleoptera, Thysanoptera, and Diptera pests on various crops such as vegetables (tomatoes, peppers, eggplants, etc.), fruit trees, potatoes, tea trees, soybeans, and cotton, while having minimal impact on mammals, beneficial arthropods, and the environment. Cyclobromamide has advantages such as a broad insecticidal spectrum, low dosage, insensitivity to temperature, and long residual effect.

[0027] Broflanilide, with the molecular formula C 25 H 14 BrF 11 N2O2, structural formula as follows:

[0028]

[0029] Brombutamide is a novel diamide insecticide. It is an allosteric regulator of GABA-gated chloride ion channels (also known as ionic GABA receptors), primarily acting on a unique binding site on this ion channel to inhibit chloride ion translocation into the cell, causing excessive excitation or spasms in insects, thus exhibiting rapid insecticidal activity. It shows good insecticidal activity against lepidopteran and coleopteran insects, such as the beet armyworm *Spodoptera litura*, the tea leafroller *Adoxophyes honmai*, the yellow-striped flea beetle *Phyllotreta striolata*, the diamondback moth *Plutella xylostella*, the rice stem borer *Chilo suppressalis*, and the rice leaf roller *Cnaphalocrocis medinalis*, especially exhibiting high larval-killing activity against lepidopteran pests, causing symptoms such as vomiting and intense excitement. Due to its high insecticidal activity against common lepidopteran and coleopteran pests and termites found on leafy vegetables and grains, it can be widely used on related crops. In addition, bromuconazole can also be used as a seed treatment agent and has good killing activity against ants, cockroaches and flies. It also has insecticidal activity against insects such as western flower thrips (Frankliniella occidentalis), cabbage caterpillar (Pieris rapae), black spiny whitefly (Aleurocanthus spiniferus), and small green leafhopper (Empoasca onukii).

[0030] Isoprocarb and methomyl belong to the carbamate insecticide class. This class of insecticides is highly selective in its application to pests, exhibiting very poor control efficacy, even zero, against lepidopteran pests. However, the technical solution that claims to have discovered the directing effect of isoprocarb on diamides has not been reported. Summary of the Invention

[0031] Purpose of the invention

[0032] The purpose of this invention is to provide an insecticidal composition and its application. The carbamate compounds of this invention can act as systemic transdermal agents for amide insecticides, thereby improving the insecticidal efficacy of amide insecticides.

[0033] Solution

[0034] To achieve the objective of this invention, in a first aspect, this invention provides an insecticidal composition, the active ingredients of which include carbamate compounds and amide insecticides in a weight ratio of (1-50):(1-50);

[0035] The carbamate compounds are selected from one or more of isoprocarb, phenoxycarb, thiamethoxam, and sec-butylcarb.

[0036] The amide insecticides mentioned are selected from one or more of bromfenac, cyclobromin, brofenac, and chlorantraniliprole.

[0037] Further, the weight ratio of carbamate compounds to amide insecticides is (1-50):(1-5), optionally (5-50):(1-5), optionally (5-50):(1-5), optionally (10-50):(1-2), optionally (10-40):(1-2), optionally (5-20):1, optionally (10-40):1, optionally (10-20):1.

[0038] Further, the carbamate compound is phenoxycarb, and the amide insecticide is selected from one or two of brofenoxam and cyclobromin; optionally, the amide insecticide is brofenoxam.

[0039] Furthermore, the carbamate compound is isoprocarb, and the amide insecticide is selected from one or two of brofenoxam and cyclobromin.

[0040] Further, the carbamate compound is thiamethoxam, and the amide insecticide is selected from one or more of bromfenac, cyclobrombutamide, brofenoxam, and chlorantraniliprole; optionally, the amide insecticide is brofenoxam.

[0041] Further, the carbamate compound is sec-butylcarbamate, and the amide insecticide is selected from one or more of brofenlanamide, cyclobrominamide, brofenlanamide, and chlorantraniliprole; optionally, the amide insecticide is brofenlanamide or chlorantraniliprole.

[0042] Furthermore, the amide insecticide is brofenoxam, and the carbamate compound is selected from one or more of isoprocarb, phenoxycarb, thiamethoxam, and sec-butylcarb.

[0043] Furthermore, the amide insecticide is cyclobrombutamide, and the carbamate compound is selected from one or more of isoprocarb, phenoxycarb, thiamethoxam, and sec-butylcarb.

[0044] Furthermore, the amide insecticide is bromocyanamide, and the carbamate compound is selected from one or more of thiamethoxam, sec-butylcarbide, isoprocarb, and phenoxycarb.

[0045] Furthermore, the amide insecticide is chlorantraniliprole, and the carbamate compound is selected from one or more of thiamethoxam, sec-butylcarbamide, isoprocarb, and phenoxycarb.

[0046] Furthermore, it also includes other pesticide-acceptable adjuvants selected from one or more of dispersants, wetting agents, emulsifiers, stabilizers, antifreeze agents, defoamers, preservatives, thickeners, solvents, organic acids, and dispersion media;

[0047] And / or, the insecticidal composition is one or more of the following: emulsifiable concentrate, microemulsion, soluble liquid, suspension concentrate, aqueous solution, water emulsion, ultra-low volume liquid, and dispersible oil suspension.

[0048] In a second aspect, the application of the insecticidal composition described in the first aspect in the control of plant pests is provided.

[0049] Further, the plant pests are lepidopteran pests and / or homoptera pests; optionally, the plant pests are one or more of the following: cotton bollworm, cotton aphid, tomato aphid, and beet armyworm.

[0050] Thirdly, a carbamate compound is provided as a systemic transdermal agent for amide insecticides, characterized in that the weight ratio of the carbamate compound to the amide insecticide is (1-50):(1-50); the carbamate compound is selected from one or more of thiamethoxam, sec-butylcarbate, phenoxycarb, and isoprocarb; and the amide insecticide is selected from one or more of bromonitrile diphenyl acetamiprid, cyclobrombutamide, bromonitrile cyantraniliprole, and chlorantraniliprole.

[0051] Fourthly, a method for improving the utilization rate of amide insecticides is provided, wherein a carbamate compound is added to the amide insecticide; the weight ratio of the carbamate compound to the amide insecticide is (1-50):(1-50), optionally, the carbamate compound is selected from one or more of thiamethoxam, sec-butylcarbide, phenoxycarb, and isoprocarb; optionally, the amide insecticide is selected from one or more of brofenoxam, cyclobromin, brofenoxam, and chlorantraniliprole.

[0052] In the first, second, third and / or fourth aspects, the weight ratio of carbamate compounds to amide insecticides is (1-50):(1-5), optionally (5-50):(1-5), optionally (5-50):(1-5), optionally (10-50):(1-2), optionally (10-40):(1-2), optionally (5-20):1, optionally (10-40):1, optionally (10-20):1.

[0053] In the first, second, third and / or fourth aspects, the carbamate compound is thiamethoxam, and the amide insecticide is selected from one or more of bromfenoxam, cyclobrombutamide, brofenoxam, and chlorantraniliprole; optionally, the amide insecticide is brofenoxam.

[0054] In the first, second, third and / or fourth aspects, the carbamate compound is sec-butylcarbamate, and the amide insecticide is selected from one or more of brofenoxam, cyclobromin, brofenoxam, and chlorantraniliprole; optionally, the amide insecticide is brofenoxam or chlorantraniliprole.

[0055] In the first, second, third and / or fourth aspects, the carbamate compound is phenoxycarb, and the amide insecticide is selected from one or two of brofenoxam and cyclobromin; optionally, the amide insecticide is brofenoxam.

[0056] In the first, second, third and / or fourth aspects, the carbamate compound is isoprocarb, and the amide insecticide is selected from one or two of brofenoxam and cyclobromin.

[0057] Beneficial effects

[0058] (1) The carbamate compounds of the present invention can be used as systemic transdermal agents for amide insecticides, which greatly improves the absorption and transduction of amide insecticides on crops, enhances the insecticidal effect of amide insecticides, improves the utilization rate of pesticides, and reduces environmental pollution.

[0059] (2) In this invention, the molecular forces of amide insecticides interact chemically with the molecules of carbamates, which assists the delivery system of amide insecticides and enhances the systemic absorption of amide insecticides on the crop surface.

[0060] (3) The carbamate and amide insecticides of this invention both contain -NH- (imine) groups. The two compounds mix to form hydrogen bonds and "π-π interactions," also known as "π-π stacking." This stacking results in the spatial orientation and ordering of the carbamate and amide insecticide molecules, allowing them to stack together in various possible configurations to form a "dimer," which in turn forms a complex. The former promotes the dissolution and penetration of the latter. Amide insecticides are thus "stacked" by carbamate compounds. Detailed Implementation

[0061] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprising of" will be understood to include the stated elements or components, without excluding other elements or other components.

[0062] Furthermore, to better illustrate the present invention, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, elements, methods, and means well known to those skilled in the art are not described in detail in order to highlight the spirit of the invention.

[0063] All pesticide technical materials used in this invention are commercially available products.

[0064] In the following examples and comparative examples, the surface tension was measured using a surface tension meter. The surface tension of the pesticide solution was measured when the surface tension of the pesticide solution was lower than the surface free energy of the target crop. Only when the surface tension of the pesticide solution was lower than the surface free energy of the target crop could the pesticide solution wet and spread on the target surface.

[0065] In the following examples and comparative examples, the liquid-solid interface contact angle was measured using a contact angle meter to determine the contact angle formed when the pesticide droplet landed on the target crop. When the liquid and solid come into contact, the free energy of the system decreases. The degree of decrease in free energy reflects the wettability of the liquid on the target crop. The smaller the contact angle, the stronger the wettability of the selected target, the better the spreadability of the pesticide on the target crop, and the higher the pesticide utilization rate.

[0066] In the following examples, the method for detecting the content of diamide insecticides in plant roots, leaves, and stems is as follows:

[0067] The analytical method used was ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). The roots, stems, and leaves of the plant were separated using scissors. The sample to be tested was collected using the quartering method. Unused samples were labeled and stored in a -20℃ freezer. 10.00 g of the sample to be tested was accurately weighed and placed in a 50 mL PTFE tube. 20 mL of a 0.08% formic acid-acetonitrile mixed solvent was added, and the mixture was vortexed for 30 min. Then, 1 g of sodium chloride and 4 g of anhydrous magnesium sulfate were added, and the mixture was shaken well and vortexed for 5 min. The mixture was then centrifuged (4000 rpm) for 5 min and purified.

[0068] Purification was performed using a dispersant adsorbent. 1.5 mL of the above extract was transferred into a 2 mL dispersion purification tube containing 150 mg of anhydrous magnesium sulfate and 25 mg of N-propylethylenediamine. The tube was vortexed for 5 min, centrifuged at 12000 r / min for 3 min, and the supernatant was filtered through a 0.22 μm filter membrane and then transferred into a pre-cut vial for analysis.

[0069] The liquid chromatography conditions were: Acquity UPLC BEH C 18 A stainless steel column was used, with a column temperature of 40℃ and a sample chamber temperature of 10℃. The injection volume was 3.0 μL. Mobile phase A was 0.05% formic acid aqueous solution, and mobile phase B was acetonitrile, with a flow rate of 0.2 μL / min. The mobile phase gradient elution program was as follows: 0–2.0 min, mobile phase A linearly decreased from 90% to 10%; 2.0–3.0 min, mobile phase A maintained at 10%; 3.0–4.0 min, mobile phase A linearly decreased from 10% to 70%; 4.0–5.0 min, mobile phase A linearly decreased from 70% to 90%.

[0070] Mass spectrometry conditions: electrospray ionization source, positive ion ionization (ESI) + The capillary voltage was 3.63 kV, the ion source temperature was 150℃, and the desolventizing temperature was 350℃. Both the desolventizing gas and the cone gas were high-purity liquid nitrogen, with a desolventizing gas flow rate of 600 L / h and a cone gas flow rate of 40 L / h. The collision gas was high-purity argon. A multi-reaction ion pair monitoring mode was employed, with qualitative analysis based on quantitative ion pairs, qualitative ion pairs, and retention time. Quantitative analysis was performed using quantitative ion pairs with good peak shape, low interference, and high response. (Reference: Determination of Brombutamide Residues in Tomatoes and Cabbage by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry)

[0071] In the following examples, in order to study whether carbamate compounds can act as systemic translocators for amide insecticides (especially diamide insecticides) or accelerate the translocation of amide insecticides (especially diamide insecticides) in plants, relevant verification experiments were conducted, and some data are as follows:

[0072] Example 1: Content determination and insecticidal effect of the targeted agent thiamethoxam combined with bromuconazole in different parts of the plant.

[0073] Example 1-1: Results of content determination of the directing agent thiamethoxam and bromuconazole in various parts of cotton plants.

[0074] Experimental Methods: The bioactivity of bromutrin dimethyl ...

[0075] Table 1-1 Results of content determination of the directing agent thiamethoxam and brofenoxam in different parts of cotton plants

[0076]

[0077] Table 1-1 shows that thiamethoxam can increase the systemic absorption of bromutsulfuron-methyl in cotton plants. The addition of the directing agent thiamethoxam significantly increases the systemic absorption of bromutsulfuron-methyl in the roots, stems, and leaves of cotton plants. The highest systemic absorption of bromutsulfuron-methyl was observed at thiamethoxam:bromutsulfuron-methyl ratios of 5:1, 10:1, and 20:1.

[0078] Example 1-2: Determination of the control effect of the combination of the directing agent thiophanate-methyl and bromofenac on cotton aphids.

[0079] Before the pot experiment, a certain amount of thiamethoxam and bromuconazole were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0080] Test Method: The systemic effect of the targeted agent thiodicarb on bromutrin dimethyl ether was determined using a pot method. Plastic pots (15×13×9cm) were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were moistened and germinated, they were sown in the plastic pots, with 4 cotton plants per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of bromutrin dimethyl ether and the targeted agent thiodicarb were mixed according to the dosages in Table 1-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with 25 cotton aphids. Four replicates were set up, with 5 pots per replicate. The number of dead aphids was checked after 48 hours. The insect population reduction rate and control efficacy were calculated, and the results are shown in Table 1-2.

[0081] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0082] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0083] Table 1-2 Efficacy of thiamethoxam and brofenoxam against cotton aphids

[0084]

[0085] As shown in Tables 1-2, the addition of the directing agent thiophanate-methyl to the root drenching treatment significantly increased the control efficacy against cotton aphids. The control efficiencies at ratios of thiophanate-methyl to bromutrin dimethyl (1:1, 5:1, 10:1, and 20:1) were all above 50% after 3 days. The highest control efficacy of 82.64% was achieved with a 20:1 ratio of thiophanate-methyl to bromutrin dimethyl as the root drenching treatment.

[0086] Results of content determination in various parts of tomato plants after the combination of the directing agent thiamethoxam and bromuconazole was achieved in Examples 1-3.

[0087] Experimental Methods: The translocation of thiophanate-methyl and bromofenac on tomato plants was tested. 100 mg / L bromofenac and the translocate agent thiophanate-methyl were mixed according to the dosage ratios in Table 1-3, and uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of bromfenac in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Tables 1-3.

[0088] Table 1-3 Results of foliar spraying with a mixture of thiamethoxam and bromofenac in tomato leaves

[0089]

[0090]

[0091] The results in Tables 1-3 indicate that the addition of the direct-directing agent thiamethoxam to brofenoxam can increase the systemic absorption of brofenoxam in tomato plants. The highest systemic absorption of brofenoxam was observed at thiamethoxam:brofenoxam ratios of 5:1, 10:1, and 20:1.

[0092] Examples 1-4: Determination of the control effect of the combined application of the directing agent thiamethoxam and bromofenopram on tomato aphids.

[0093] Before the pot experiment, a certain amount of thiamethoxam and bromuconazole were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0094] Measurement method: The experiment was conducted in fields with severe tomato aphid infestations. The treatment concentrations were diluted with water and sprayed at a rate of 1000 m² per 667 m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 3 tomato plants sampled at each point, and the insect population count recorded. Further surveys were conducted 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy.

[0095] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0096] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0097] Table 1-4 Field efficacy results of thiamethoxam and bromofenac in treating tomato aphids.

[0098]

[0099]

[0100] As can be seen from the data in Table 1-4, the control efficacy of bromoxynil dimethyl sulfadiazine against tomato aphids was significantly increased after adding the directing agent thiamethoxam. The highest control efficacy (86.78%) was observed after 3 days of treatment with a ratio of thiamethoxam:bromoxynil dimethyl sulfadiazine = 20:1.

[0101] Example 2: Content determination and insecticidal effect of the targeted agent sec-butylcarbamide combined with bromuconazole in various parts of the plant.

[0102] Example 2-1: Results of content determination of the directing agent sec-butylcarbate and bromuconazole in various parts of cotton plants.

[0103] Experimental Methods: The bioactivity of brofentanil in cotton plants was determined using a pot experiment. Cotton plants were planted in 15×13×9cm plastic pots, with 4 plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Brofentanil and tebuconazole were mixed according to the dosages in Table 2-1 and applied as a root drenching treatment to the cotton plants, with 50 mL of the agent per plant. Four replicate plots were set up, with 5 pots in each plot. The content of brofentanil in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 2-1.

[0104] Table 2-1 Results of content determination of the directing agent sec-butylcarbate and bromofenac in different parts of cotton plants

[0105]

[0106]

[0107] Table 2-1 shows that the directing agent sec-butylcarbide can increase the absorption of bromutsulfuron-methyl itself in cotton plants. The addition of sec-butylcarbide significantly increases the systemic absorption of bromutsulfuron-methyl in the roots, stems, and leaves of cotton plants. Among these, the treatments with sec-butylcarbide:bromutsulfuron-methyl ratios of 5:1, 10:1, and 20:1 resulted in the highest systemic absorption of bromutsulfuron-methyl.

[0108] Example 2-2: Determination of the control effect of the combination of the directing agent sec-butylcarbamide and bromofenac on cotton bollworm.

[0109] Before the pot experiment, a certain amount of sec-butylcarbamate and bromuconazole were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0110] Methods: The systemic effect of the targeted agent tebufenozide on bromutrin dimethyl ether was determined using a pot method. Plastic pots (15×13×9cm) were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were moistened and germinated, they were sown in the plastic pots, four plants per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of bromutrin dimethyl ether and the targeted agent tebufenozide were mixed according to the dosages in Table 2-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with two second-instar bollworms. Four replicates were set up, with five pots per replicate. The number of dead bollworms was checked after 48 hours. The pest population reduction rate and control efficacy were calculated, and the results are shown in Table 2-2.

[0111] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0112] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0113] Table 2-2 Efficacy of bromofenac in controlling cotton bollworm

[0114]

[0115] As can be seen from Table 2-2, the root drenching treatment with the addition of the directing agent sec-butylcarbide significantly increased the control efficacy against cotton bollworm. The root drenching treatment with a ratio of sec-butylcarbide to brombutamide of 20:1 achieved the highest control efficacy of 63.13% after 3 days.

[0116] Example 2-3: Determination of systemic translocation of the directing agent sec-butylcarbate combined with bromuconazole in tomato plant leaves.

[0117] Experimental Methods: The translocation of tebufenozide and brofenoxam on tomato plants was tested. 100 mg / L brofenozide and the translocate agent tebufenozide were mixed according to the dosage ratios in Table 2-3, and uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of bromfenac in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 2-3.

[0118] Table 2-3 Results of foliar spraying of tomato plant leaves with a mixture of methylparaben and bromofenac.

[0119] The results in Table 2-3 indicate that the addition of the directing agent tebuconazole to brofenoxam can increase the systemic absorption of brofenoxam in tomato plants. Among these, the highest systemic absorption of brofenoxam was observed after 5 days of treatment with tebuconazole:brofenoxam ratios of 5:1, 10:1, and 20:1.

[0120] Example 2-4: Determination of the control effect of the targeted agent tebuconazole combined with bromofenac on tomato cutworm.

[0121] Before the experiment, weigh out a certain amount of sec-butylcarbamide and bromuconazole, dissolve them in acetone, and put them into volumetric flasks for later use.

[0122] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Further surveys were conducted 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy.

[0123] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0124] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0125] Table 2-4 Field efficacy results of bromofenaciocarbamate against the tomato cutworm.

[0126]

[0127]

[0128] As can be seen from the data in Table 2-4, the control efficacy of cyclochlorfenapyr against tomato cutworm was significantly increased after the addition of the directing agent sec-butylcarbide. The highest control efficacy of 83.33% was achieved in the sec-butylcarbide:cyclochlorfenapyr = 20:1 treatment at 3 days.

[0129] Example 3: Content determination results and insecticidal effect of the combined direct-targeting agent phenoxycarb and bromoxynil dimethyl methacrylate in different parts of the plant.

[0130] Example 3-1: Content determination results of the directing agent phenoxycarb and bromofenac in various parts of cotton plants.

[0131] Experimental Methods: The bioactivity of bromofenac in cotton plants was determined using a pot experiment. Cotton plants were planted in 15×13×9cm plastic pots, with 4 plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Bromofenac and phenoxycarb were mixed according to the dosages in Table 3-1, and 50 mL of the agent was applied to the roots of the cotton plants as a root drenching treatment. Four replicate plots were set up, with 5 pots in each plot. The content of bromofenac in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 3-1.

[0132] Table 3-1 Results of content determination of the directing agent phenoxycarb and bromofenac in different parts of cotton plants

[0133]

[0134]

[0135] Table 3-1 shows that phenoxycarb can increase the systemic absorption of brofenoxam in cotton plants. The addition of the directing agent phenoxycarb significantly increased the systemic absorption of brofenoxam in the roots, stems, and leaves of cotton plants. The highest systemic absorption of brofenoxam was observed at phenoxycarb:brofenoxam ratios of 5:1, 10:1, and 20:1.

[0136] Example 3-2: Determination of the control effect of the combination of the directing agent phenoxycarb and bromoxynil dimethamide on cotton bollworm.

[0137] Before the pot experiment, a certain amount of phenoxycarb and bromoxynil were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0138] Test Method: The systemic effect of the directing agent phenoxycarb on bromutrin dimethyl ether was determined using a pot method. Plastic pots (15×13×9cm) were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were moistened and germinated, they were sown in the plastic pots, four plants per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of bromutrin dimethyl ether and the directing agent phenoxycarb were mixed according to the dosages in Table 3-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with two second-instar bollworms. Four replicates were set up, with five pots per replicate. The number of dead bollworms was checked after 48 hours. The pest population reduction rate and control efficacy were calculated, and the results are shown in Table 3-2.

[0139] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0140] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0141] Table 3-2 Control efficacy of phenoxycarb and bromofenac against cotton bollworm.

[0142] As can be seen from Table 3-2, the root drenching treatment with the addition of the directing agent phenoxycarb significantly increased the control efficacy against cotton bollworm. The highest control efficacy of 81.25% was achieved after 3 days when the ratio of directing agent phenoxycarb to brombutamide was 20:1.

[0143] Example 3-3: Determination of systemic translocation in tomato plant leaves using a combination of the directing agent phenoxycarb and bromonitrile dimethamide.

[0144] Experimental Methods: The translocation of phenoxycarb and brofenoxam on tomato plants was tested. 100 mg / L brofenoxam and the translocator phenoxycarb were mixed according to the dosage ratios in Table 3-3, and the mixture was uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of bromfenac in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 3-2.

[0145] Table 3-3 Results of foliar spraying of tomato plant leaves with a mixture of phenoxycarb and bromofenac.

[0146] The results in Table 3-3 indicate that the addition of the directing agent phenoxycarb to brofenoxam can increase the systemic absorption of brofenoxam in tomato plants. Among them, the systemic absorption of brofenoxam was highest after 5 days of treatment with phenoxycarb:brofenoxam ratios of 5:1, 10:1, and 20:1.

[0147] Example 3-4: Determination of the control effect of the combined directing agent phenoxycarb and bromonitrile dimethamide on tomato beet armyworm.

[0148] Before the pot experiment, a certain amount of phenoxycarb and bromoxynil were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0149] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Further surveys were conducted 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy.

[0150] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0151] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0152] Table 3-4 Field efficacy results of bromofenaciocarbamate against the tomato cutworm.

[0153]

[0154] As can be seen from the data in Table 3-4, the control efficacy of bromoxynil dimethyl methacrylate (BMF) against tomato cutworm was significantly increased after adding the directing agent phenoxycarb. Among them, the highest control efficacy of 92.06% was achieved after 3 days when the ratio of methyl methacrylate to bromoxynil dimethyl methacrylate (20:1) was used for foliar spraying.

[0155] Example 4: Content determination results and insecticidal effect of the combined targeted agent isoprocarb and brofenoxam in various parts of the plant. Example 4-1: Content determination results of the combined targeted agent isoprocarb and brofenoxam in various parts of the cotton plant.

[0156] Experimental Methods: The bioactivity of brofentanil in cotton plants was determined using a pot experiment. Cotton plants were planted in 15×13×9cm plastic pots, with 4 plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Brofentanil and isoprocarb were mixed according to the dosages in Table 4-1, and 50 mL of the agent was applied to the roots of the cotton plants as a root drenching treatment. Four replicate plots were set up, with 5 pots in each plot. The content of brofentanil in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 4-1.

[0157] Table 4-1 Results of content determination of the directing agent isoprocarb and brofenoxam in different parts of cotton plants

[0158]

[0159]

[0160] Table 4-1 shows that isoprocarb can increase the systemic absorption of brofentanil in cotton plants. The addition of the directing agent isoprocarb significantly increases the systemic absorption of brofentanil in the roots, stems, and leaves of cotton plants. The highest systemic absorption of brofentanil was observed at isoprocarb:brofentanil ratios of 5:1, 10:1, and 20:1.

[0161] Example 4-2: Determination of the control effect of the combination of the directing agent isoprocarb and bromofenac dimethyl ether on cotton bollworm.

[0162] Before the pot experiment, a certain amount of isoprocarb and bromoxynil were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0163] Test Method: The systemic effect of the targeted agent isoprocarb on bromutrin dimethyl ...

[0164] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0165] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0166] Table 4-2 Control efficacy of isoprocarb and bromofenac against cotton bollworm.

[0167]

[0168] As shown in Table 4-2, the addition of the directing agent isoprocarb to the root drenching treatment significantly increased the control efficacy against cotton bollworm. The highest control efficacy of 88.13% was achieved after 3 days when the ratio of isoprocarb to brofentanil was 20:1.

[0169] Example 4-3: Determination of systemic translocation in tomato plant leaves using a combination of the directing agent isoprocarb and brofenoxam.

[0170] Experimental Methods: A test of the translocation properties of isoprocarb and brofenoxam on tomato plants was conducted. 100 mg / L brofenoxam and the translocator isoprocarb were mixed according to the dosage ratios shown in Table 4-3. The mixture was then uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of bromfenac in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 4-3.

[0171] Table 4-3 Results of foliar spraying with a mixture of isoprocarb and bromofenac in tomato leaves

[0172] The results in Table 4-3 indicate that the addition of the directing agent isoprocarb to brofenoxam can increase the systemic absorption of brofenoxam in tomato plants. The highest systemic absorption of brofenoxam was observed after 5 days of treatment with isoprocarb:brofenoxam ratios of 5:1, 10:1, and 20:1.

[0173] Example 4-4: Determination of the control effect of the combination of the directing agent isoprocarb and brofenoxam on tomato beet armyworm.

[0174] Before the pot experiment, a certain amount of isoprocarb and bromoxynil were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0175] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Further surveys were conducted 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy.

[0176] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0177] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0178] Table 4-4 Field efficacy results of bromofenaciocarbamate against the tomato cutworm.

[0179]

[0180] As can be seen from the data in Table 4-4, the control efficacy of brofenoxam with the addition of the directing agent isoprocarb significantly increased against tomato cutworm. Among them, the highest control efficacy of 98.15% was achieved after 3 days when the foliar spray treatment was 20:1 of brofenoxam and 20:1.

[0181] Example 5: Content determination and insecticidal effect of the targeted agent thiamethoxam combined with cyclochlorfenapyr in various parts of the plant.

[0182] Example 5-1: Results of content determination of the directing agent thiamethoxam and cyclochlorfenapyr in various parts of cotton plants.

[0183] Experimental Methods: The bioactivity of cyclochlorfenapyr in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with two plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Cyclochlorfenapyr and thiamethoxam were mixed according to the dosages in Table 5-1 and applied as a root drenching treatment to the cotton plants, with 50 mL of the agent per plant. Four replicate plots were set up, with five pots in each plot. The content of cyclochlorfenapyr in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 5-1.

[0184] Table 5-1 Results of content determination of the directing agent thiamethoxam and cyclochlorfenapyr in different parts of cotton plants

[0185]

[0186]

[0187] Table 5-1 shows that thiamethoxam can increase the systemic absorption of cyclochlorfenapyr in cotton plants. The addition of the directing agent thiamethoxam significantly increases the systemic absorption of cyclochlorfenapyr in the roots, stems, and leaves of cotton plants. Among these, the treatments with thiamethoxam:cyclochlorfenapyr ratios of 5:1, 10:1, and 20:1 resulted in the highest systemic absorption of cyclochlorfenapyr.

[0188] Example 5-2: Determination of the control effect of the combination of the directing agent thiamethoxam and cyclobrombutamide on cotton aphids.

[0189] Before the pot experiment, a certain amount of thiamethoxam and cyclophosphamide were weighed out, dissolved in acetone, and placed in volumetric flasks for later use.

[0190] Methods: The systemic effect of the targeted agent thiamethoxam on cyclophosphamide was determined using a pot experiment. Plastic pots (15×13×9cm) were used, each filled with soil that had passed through a 200-mesh sieve. Cotton plants were moistened and germinated before being sown in the pots, two seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of cyclophosphamide and the targeted agent thiamethoxam were mixed according to the dosages in Table 5-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with 50 cotton aphids. Four replicates were set up, with five pots per replicate. The number of dead aphids was checked after 48 hours. The insect population reduction rate and control efficacy were calculated, and the results are shown in Table 5-2.

[0191] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0192] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0193] Table 5-2 Control efficacy of thiamethoxam and cyclobrombutamide against cotton aphids.

[0194]

[0195]

[0196] As shown in Table 5-2, the addition of the directing agent thiophanate-methyl to cyclophosphamide during root drenching significantly increased the control efficacy against cotton aphids. The control efficacy at 3-day levels was higher than 50% for treatments with a thiophanate-methyl:cyclophosphamide ratio of 5:1, 10:1, and 20:1. The highest control efficacy of 84.13% was achieved at a thiophanate-methyl:cyclophosphamide ratio of 20:1 during root drenching.

[0197] Example 5-3: Determination of systemic translocation of the directing agent thiamethoxam and cyclophosphamide in tomato plant leaves.

[0198] Experimental Methods: A test was conducted on the translocation of thiamethoxam and cyclophosphamide on tomato plants. 100 mg / L cyclophosphamide and the translocate agent thiamethoxam were mixed according to the dosage ratios in Table 5-3, and the mixture was uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of cyclophosphamide in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 5-3.

[0199] Table 5-3 Results of foliar spraying of tomato plant leaves with a mixture of thiamethoxam and cyclophosphamide

[0200]

[0201] The results in Table 5-3 indicate that the addition of the direct-directing agent thiamethoxam to cyclochlorfenapyr can increase the systemic absorption of cyclochlorfenapyr in tomato plants. The highest systemic absorption of cyclochlorfenapyr was observed after 1 day of treatment with thiamethoxam:cyclochlorfenapyr ratios of 5:1, 10:1, and 20:1.

[0202] Example 5-4: Determination of the control effect of the combination of the directing agent thiamethoxam and cyclophosphamide on tomato aphids.

[0203] Before the pot experiment, a certain amount of thiamethoxam and cyclophosphamide were weighed out, dissolved in acetone, and placed in volumetric flasks for later use.

[0204] Measurement method: The experiment was conducted in fields with severe tomato aphid infestations. The treatment concentrations were diluted with water and sprayed at a rate of 1000 m² per 667 m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Further surveys were conducted 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy.

[0205] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0206] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0207] Table 5-4 Field efficacy results of cyclochlorfenapyr against tomato aphids.

[0208]

[0209] As can be seen from the data in Table 5-4, the control efficacy of cyclobrombutamide against tomato aphids was significantly increased after adding the directing agent thiamethoxam. The highest control efficacy (94.34%) was observed after 3 days of treatment with a ratio of thiamethoxam:cyclobrombutamide of 20:1.

[0210] Example 6: Content determination and insecticidal effect of the targeted agent tebuconazole combined with cyclochlorfenapyr in different parts of the plant.

[0211] Example 6-1: Results of content determination of the directing agent sec-butylcarbate and cyclochlorfenapyr in different parts of cotton plants.

[0212] Experimental Methods: The bioactivity of cyclochlorfenapyr in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with two plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Cyclochlorfenapyr and isoprocarb were mixed according to the dosages in Table 6-1, and the cotton plants were treated with 50mL of the agent per plant by root drenching. Four replicate plots were set up, with five pots in each plot. The content of cyclochlorfenapyr in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 6-1.

[0213] Table 6-1 Results of content determination of the directing agents sec-butylcarbamate and cyclochlorfenapyr in different parts of cotton plants

[0214]

[0215]

[0216] Table 6-1 shows that the directing agent sec-butylcarbate can increase the absorption of cyclochlorfenapyr itself in cotton plants. The addition of sec-butylcarbate significantly increases the systemic absorption of cyclochlorfenapyr in the roots, stems, and leaves of cotton plants. Among these, the treatments with isoprocarb:cyclochlorfenapyr ratios of 5:1, 10:1, and 20:1 resulted in the highest systemic absorption of cyclochlorfenapyr.

[0217] Example 6-2: Determination of the control effect of the combined application of the directing agent tebuconazole and cyclophosphamide on cotton bollworm.

[0218] Before the pot experiment, a certain amount of sec-butylcarbamate and cyclophosphamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0219] Test Method: The systemic effect of the targeted agent tebufenozide on cyclophosphamide was determined using a pot method. 15×13×9cm plastic pots were used, each filled with soil that had passed through a 200-mesh sieve. Cotton plants were moistened and germinated before being sown in the pots, two seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of cyclophosphamide and the targeted agent tebufenozide were mixed according to the dosages in Table 41. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with two second-instar bollworms. Four replicates were set up, with five pots per replicate. The number of dead bollworms was checked after 72 hours. The pest population reduction rate and control efficacy were calculated.

[0220] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0221] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0222] Table 6-2 Control efficacy of chlorfenapyr and cyclophosphamide against cotton bollworm

[0223]

[0224]

[0225] As shown in Table 6-2, the addition of the directing agent tebuconazole to the root drenching treatment significantly increased the control efficacy against cotton bollworm. The control efficiencies were all above 50% in treatments with a tebuconazole:cyclochlorfenapyr ratio of 5:1, 10:1, and 20:1. The highest control efficacy (80.63%) was observed after 3 days with a tebuconazole:cyclochlorfenapyr ratio of 20:1.

[0226] Example 6-3: Determination of systemic translocation of the directing agents sec-butylcarbate and cyclophosphamide in tomato plant leaves.

[0227] Experimental Methods: A test was conducted on the translocation of chlorfenapyr and cyclophosphamide on tomato plants. 100 mg / L cyclophosphamide and the translocate agent chlorfenapyr were mixed according to the dosage ratios shown in Table 6-2. The mixture was then uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of cyclophosphamide in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 6-3.

[0228] Table 6-3 Results of foliar spraying of tomato plant leaves with a mixture of methyl parathion and cyclophosphamide

[0229] The results in Table 6-3 indicate that the addition of the directing agent tebuconazole to cyclochlorfenapyr can increase the systemic absorption of cyclochlorfenapyr in tomato plants. Among the ratios of tebuconazole to cyclochlorfenapyr, 5:1, 10:1, and 20:1, the systemic absorption of cyclochlorfenapyr was the highest.

[0230] Example 6-4: Determination of the control effect of the combined application of the directing agent tebufenozide and cyclophosphamide on tomato beet armyworm.

[0231] Before the pot experiment, a certain amount of sec-butylcarbamate and cyclophosphamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0232] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before application of pesticides, the initial insect population was investigated using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Surveys were conducted again at 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy. The results are shown in 6-4.

[0233] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0234] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0235] Table 6-4 Field efficacy results of cyclochlorfenapyr against tomato cutworm.

[0236]

[0237] As can be seen from the data in Table 56, the control efficacy of cyclochlorfenapyr against tomato cutworm was significantly increased after the addition of the directing agent sec. Butyr. carbamate. The highest control efficacy of 83.75% was achieved in the 3-day treatment with a ratio of sec. butyr:cyclochlorfenapyr = 20:1.

[0238] Example 7: Results of content determination in various parts of the plant after the directing agent phenoxycarb and cyclobrombutamide were combined.

[0239] Example 7-1: Results of content determination of the directing agent phenoxycarb and cyclochlorfenapyr in various parts of cotton plants.

[0240] Experimental Methods: The bioactivity of cyclochlorfenapyr in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with two plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Cyclochlorfenapyr and phenoxycarb were mixed according to the dosages in Table 7-1, and the cotton plants were treated with root irrigation using 50mL of the agent per plant. Four replicate plots were set up, with five pots in each plot. The content of cyclochlorfenapyr in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 7-1.

[0241] Table 7-1 Results of content determination of the directing agent phenoxycarb and cyclobrombutamide in different parts of cotton plants

[0242]

[0243]

[0244] Table 7-1 shows that phenoxycarb can increase the systemic absorption of cyclochlorfenapyr in cotton plants. The addition of the directing agent phenoxycarb significantly increased the systemic absorption of cyclochlorfenapyr in the roots, stems, and leaves of cotton plants. The highest systemic absorption of cyclochlorfenapyr was observed at phenoxycarb:cyclochlorfenapyr ratios of 5:1, 10:1, and 20:1.

[0245] Example 7-2: Determination of the control effect of the combined application of the directing agent phenoxycarb and cyclobrombutamide on cotton bollworm.

[0246] Before the pot experiment, a certain amount of phenoxycarb and cyclobrombutamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0247] Methods: The systemic effect of the directing agent phenoxycarb on cyclophosphamide was determined using a pot method. 15×13×9cm plastic pots were used, each filled with soil that had passed through a 200-mesh sieve. Cotton plants were moistened and germinated before being sown in the pots, two seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of cyclophosphamide and the directing agent phenoxycarb were mixed according to the dosages in Table 7-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with two second-instar bollworms. Four replicates were set up, with five pots per replicate. The number of dead bollworms was checked after 48 hours. The pest population reduction rate and control efficacy were calculated, and the results are shown in Table 7-2.

[0248] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0249] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0250] Table 7-2 Efficacy of Cyclofenac against Cotton Bollworm

[0251]

[0252]

[0253] As can be seen from Table 7-2, the root drenching treatment with the addition of the directing agent phenoxycarb significantly increased the control efficacy against cotton bollworm. The highest control efficacy of 76.25% was achieved after 3 days when the ratio of directing agent phenoxycarb to cyclobrombutamide was 20:1.

[0254] Example 7-3: Determination of systemic translocation of the directing agent phenoxycarb and cyclobrombutamide in tomato plant leaves.

[0255] Experimental Methods: The translocation test of phenoxycarb and cyclochlorfenapyr on tomato plants was conducted. 100 mg / L cyclochlorfenapyr and the translocate agent phenoxycarb were mixed according to the dosage ratio in Table 7-3, and the mixture was uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of cyclophosphamide in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 7-3.

[0256] Table 7-3 Results of foliar spraying of phenoxycarb and cyclochlorfenapyr in tomato plant leaves

[0257] The results in Table 7-3 indicate that the addition of the directing agent phenoxycarb to cyclochlorfenapyr can increase the systemic absorption of cyclochlorfenapyr in tomato plants. Among these, the systemic absorption of cyclochlorfenapyr was highest with mixtures of phenoxycarb:cyclochlorfenapyr at ratios of 5:1, 10:1, and 20:1.

[0258] Example 7-4: Determination of the control effect of the combined application of the directing agent phenoxycarb and cyclobrombutamide on tomato beet armyworm.

[0259] Before the pot experiment, a certain amount of phenoxycarb and cyclobrombutamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0260] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Further surveys were conducted 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy.

[0261] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0262] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0263] Table 7-4 Field efficacy results of cyclochlorfenapyr against tomato cutworm.

[0264]

[0265] As can be seen from the data in Table 7-4, the control efficacy of cyclochlorfenapyr against tomato cutworm was significantly increased after adding the directing agent phenoxycarb. Among them, the foliar spray treatment with a ratio of 20:1 of cyclochlorfenapyr to bismuth subtilis showed the highest control efficacy of 79.35% after 3 days.

[0266] Example 8: Content determination and insecticidal effect of the targeted agent isoprocarb combined with cyclochlorfenapyr in different parts of the plant.

[0267] Example 8-1: Determination of the content of the directing agent isoprocarb and cyclochlorfenapyr in different parts of cotton plants.

[0268] Experimental Methods: The bioactivity of cyclochlorfenapyr in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with two plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Cyclochlorfenapyr and isoprocarb were mixed according to the dosages in Table 8-1, and the cotton plants were treated with 50mL of the mixture as a root drenching. Four replicate plots were set up, with five pots in each plot. The content of cyclochlorfenapyr in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 8-1.

[0269] Table 8-1 Results of content determination of the directing agents isoprocarb and cyclochlorfenapyr in different parts of cotton plants

[0270]

[0271]

[0272] Table 8-1 shows that isoprocarb can increase the systemic absorption of cyclochlorfenapyr in cotton plants. The addition of the directing agent isoprocarb significantly increases the systemic absorption of cyclochlorfenapyr in the roots, stems, and leaves of cotton plants. The highest systemic absorption of cyclochlorfenapyr was observed at isoprocarb:cyclochlorfenapyr ratios of 5:1, 10:1, and 20:1.

[0273] Example 8-2: Determination of the control effect of the combination of the directing agent isoprocarb and cyclochlorfenapyr on cotton bollworm.

[0274] Before the pot experiment, a certain amount of isoprocarb and cyclobrombutamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0275] Test Method: The systemic effect of the targeted agent isoprocarb on cyclochlorfenapyr was determined using a pot method. Plastic pots (15×13×9cm) were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were kept moist and germinated, they were sown in the plastic pots, 4 seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of cyclochlorfenapyr and the targeted agent isoprocarb were mixed according to the dosages in Table 8-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with 2 second-instar bollworms. Four replicates were set up, with 5 pots per replicate. The number of dead bollworms was checked after 48 hours. The pest population reduction rate and control efficacy were calculated, and the results are shown in Table 8-2.

[0276] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0277] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0278] Table 8-2 Control efficacy of isoprocarb and cyclobrombutamide against cotton bollworm

[0279]

[0280]

[0281] As shown in Table 8-2, the addition of the directing agent isoprocarb to the root drenching treatment significantly increased the control efficacy against cotton bollworm. The control efficiencies of the isoprocarb:cyclobromide ratios of 5:1, 10:1, and 20:1 were all above 50%. The highest control efficacy of 90.51% was achieved after 3 days with the isoprocarb:cyclobromide ratio of 20:1.

[0282] Example 8-3: Determination of systemic translocation of the directing agents isoprocarb and cyclochlorfenapyr in tomato plant leaves.

[0283] Experimental Methods: The translocation of isoprocarb and cyclopropamide on tomato plants was tested. 100 mg / L cyclopropamide and the translocate agent isoprocarb were mixed according to the dosage ratio in Table 8-3, and uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of cyclophosphamide in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 8-3.

[0284] Table 8-3 Results of foliar spraying of isoprocarb and cyclochlorfenapyr in tomato plant leaves

[0285] The results in Table 8-3 indicate that the addition of the directing agent isoprocarb to cyclochlorfenapyr can increase the systemic absorption of cyclochlorfenapyr in tomato plants. Among these, the systemic absorption of cyclochlorfenapyr was highest with mixtures of isoprocarb:cyclochlorfenapyr at ratios of 5:1, 10:1, and 20:1.

[0286] Example 8-4: Determination of the control effect of the combined application of the directing agent isoprocarb and cyclobrombutamide on tomato beet armyworm.

[0287] Before the pot experiment, a certain amount of isoprocarb and cyclobrombutamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0288] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2The blocks were randomly arranged among the groups. Before applying the pesticide, the initial insect population was investigated using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Surveys were conducted again at 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy. The results are shown in Table 8-4.

[0289] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0290] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0291] Table 8-4 Field efficacy results of cyclochlorfenapyr against tomato cutworm.

[0292]

[0293] As can be seen from the data in Table 57, the control efficacy of cyclochlorfenapyr against tomato cutworm was significantly increased after adding the directing agent isoprocarb. Among them, the foliar spray treatment with a ratio of 10:1 of cyclochlorfenapyr to sec. showed the highest control efficacy of 95.26% after 3 days.

[0294] Example 9: Content determination and insecticidal effect of the targeted agent thiamethoxam combined with bromocyanamide in different parts of the plant.

[0295] Example 9-1: Results of content determination of the directing agent thiamethoxam and bromocyanamide in various parts of cotton plants.

[0296] Experimental Methods: The bioactivity of cyantraniliprole in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with 4 plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Cyantraniliprole and thiamethoxam were mixed according to the dosages in Table 9-1, and 50 mL of the agent was applied to the roots of the cotton plants as a root drenching treatment. Four replicate plots were set up, with 5 pots in each plot. The content of cyantraniliprole in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 9-1.

[0297] Table 9-1 Results of content determination of the directing agent thiamethoxam and bromocyanamide in different parts of cotton plants

[0298]

[0299]

[0300] Table 9-1 shows that thiamethoxam can increase the systemic absorption of cyantraniliprole in cotton plants. The addition of the directing agent thiamethoxam significantly increases the systemic absorption of cyantraniliprole in the roots, stems, and leaves of cotton plants. The highest systemic absorption of cyantraniliprole was observed at thiamethoxam:cyantraniliprole ratios of 5:1, 10:1, and 20:1.

[0301] Example 9-2: Determination of the control effect of the combination of the directing agent thiamethoxam and bromocyanamide on cotton aphids.

[0302] Before the pot experiment, a certain amount of thiamethoxam and bromocyanamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0303] Methods: The systemic effect of the targeted agent thiamethoxam on cyantraniliprole was determined using a pot experiment. 15×13×9cm plastic pots were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were kept moist and germinated, they were sown in the plastic pots, 4 seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of cyantraniliprole and the targeted agent thiamethoxam were mixed according to the dosages in Table 9-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to the roots of each cotton plant for root irrigation. 24 hours later, each cotton plant was inoculated with 50 cotton aphids. Four replicates were set up, with 5 pots per replicate. The number of dead aphids was checked after 48 hours. The insect population reduction rate and control efficacy were calculated, and the results are shown in Table 9-2.

[0304] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0305] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0306] Table 9-2 Efficacy of bromocyanamide against cotton aphids

[0307]

[0308]

[0309] As shown in Table 9-2, the addition of the directing agent thiophanate-methyl to the root drenching treatment significantly increased the control efficacy against cotton aphids. The control efficiencies at ratios of thiophanate-methyl:bromocyanamide of 1:1, 5:1, 10:1, and 20:1 were all above 75% after 3 days. The highest control efficacy of 87.04% was achieved with a thiophanate-methyl:bromocyanamide ratio of 20:1.

[0310] Example 9-3: Determination of systemic translocation of the directing agent thiamethoxam and bromocyanamide in tomato plant leaves.

[0311] Experimental Methods: The translocation test of thiamethoxam and fenpropathrin on tomato plants was conducted. 100 mg / L fenpropathrin and the translocate agent thiamethoxam were mixed according to the dosage ratio in Table 9-3, and uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of bromocyanamide in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 9-3.

[0312] Table 9-3 Results of foliar spraying of tomato plant leaves with a mixture of thiamethoxam and bromocyanamide

[0313] The results in Table 9-3 indicate that the addition of the direct-directing agent thiamethoxam to broflanilide can increase the systemic absorption of broflanilide in tomato plants. The highest systemic absorption of broflanilide was observed after 5 days of treatment with thiamethoxam:broflanilide ratios of 5:1, 10:1, and 20:1.

[0314] Example 9-4: Determination of the control effect of the combination of the directing agent thiamethoxam and bromocyanamide on tomato aphids.

[0315] Before the pot experiment, a certain amount of thiamethoxam and bromocyanamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0316] Measurement method: The experiment was conducted in fields with severe tomato aphid infestations. The treatment concentrations were diluted with water and sprayed at a rate of 1000 m² per 667 m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before applying the pesticide, the initial insect population was investigated using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. Surveys were conducted again at 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy. The results are shown in Table 9-4.

[0317] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0318] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0319] Table 9-4 Field efficacy results of bromocyanamide against tomato aphids.

[0320]

[0321] As can be seen from the data in Table 9-4, the control efficacy of bromocyanamide against tomato aphids was significantly increased after adding the directing agent thiamethoxam. The 3-day control efficacy of the thiamethoxam:bromocyanamide = 5:1, 10:1 and 20:1 treatments all reached 89.07%.

[0322] Example 10: Content determination and insecticidal effect of the targeted agent tebuconazole combined with bromocyanamide in different parts of the plant.

[0323] Example 10-1: Results of content determination of the directing agent sec-butylcarbate and bromocyanamide in various parts of cotton plants.

[0324] Experimental Methods: The translocation of cyantraniliprole in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with two plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Cyantraniliprole and tebuconazole were mixed according to the dosages in Table 10-1, and the cotton plants were treated with root irrigation at a rate of 50 mL per plant. Four replicate plots were set up, with five pots in each plot. The content of cyantraniliprole in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 10-1.

[0325] Table 10-1 Results of content determination of the directing agents sec-butylcarbate and bromocyanamide in different parts of cotton plants

[0326]

[0327]

[0328] Table 10-1 shows that the directing agent tebuconazole can increase the absorption of cyantraniliprole itself in cotton plants. The addition of tebuconazole significantly increases the systemic absorption of cyantraniliprole in the roots, stems, and leaves of cotton plants. Among these, the treatments with tebuconazole:cyantraniliprole ratios of 5:1, 10:1, and 20:1 resulted in the highest systemic absorption of cyantraniliprole.

[0329] Example 10-2: Determination of the control effect of the combination of the directing agent tebuconazole and bromocyanamide on cotton bollworm.

[0330] Before the pot experiment, a certain amount of sec-butylcarbamate and bromocyanamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0331] Test Method: The systemic effect of the targeted agent tebufenozide on cyantraniliprole was determined using a pot method. 15×13×9cm plastic pots were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were kept moist and germinated, they were sown in the plastic pots, 4 seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of cyantraniliprole and the targeted agent tebufenozide were mixed according to the dosage in Table 10-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with 2 second-instar bollworms. Four replicates were set up, with 5 pots per replicate. The number of dead bollworms was checked after 48 hours. The pest population reduction rate and control efficacy were calculated, as shown in Table 10-2.

[0332] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0333] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0334] Table 10-2 Efficacy of cyantraniliprole against cotton bollworm

[0335]

[0336]

[0337] As shown in Table 10-2, the addition of the directing agent tebuconazole to the root drenching treatment significantly increased the control efficacy against cotton bollworm. The control efficiencies of the tebuconazole:brobuconazole ratios of 1:1, 5:1, 10:1, and 20:1 were all above 80%. The highest control efficacy (85.63%) was observed after 3 days with the tebuconazole:brobuconazole ratio of 20:1.

[0338] Example 10-3 Results of content determination in tomato plant leaves after the combination of the directing agent sec-butylcarbate and bromocyanamide.

[0339] Experimental Methods: A test was conducted on the translocation of tebuconazole and cypermethrin on tomato plants. 100 mg / L cypermethrin and the translocate agent tebuconazole were mixed according to the dosage ratio in Table 10-3, and the mixture was uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of bromocyanamide in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 10-3.

[0340] Table 10-3 Results of foliar spraying of tomato plant leaves after the combination of methylparaben and bromocyanamide

[0341]

[0342]

[0343] The results in Table 10-3 indicate that the addition of the directing agent tebuconazole to cyantraniliprole can increase the systemic absorption of cyantraniliprole in tomato plants. Among them, the systemic absorption of cyantraniliprole was highest after 5 days of treatment with tebuconazole:cyantraniliprole ratios of 5:1, 10:1, and 20:1.

[0344] Example 10-4: Determination of the control effect of the combined application of the directing agent tebuconazole and bromocyanamide on tomato cutworm.

[0345] Before the pot experiment, a certain amount of sec-butylcarbamate and bromocyanamide were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0346] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2 The blocks were randomly arranged among the groups. Before applying the pesticide, the initial insect population was investigated using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count was recorded. Surveys were conducted again at 1 day, 3 days, and 7 days after application to calculate the insect population reduction rate and control efficacy. The results are shown in Table 10-4.

[0347] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0348] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0349] Table 10-4 Field efficacy results of bromocyanamide against tomato cutworm.

[0350]

[0351] As can be seen from the data in Table 10-4, the control efficacy of bromocyanamide against tomato cutworm was significantly increased after adding the directing agent sec. The control efficacy was highest at 84.18% 3 days after foliar spraying when the ratio of directing agent sec.sec:bromocyanamide = 10:1.

[0352] Example 11: Content determination and insecticidal effect of the combined direct-acting agent sec-butylcarbate and chlorantraniliprole in different parts of the plant.

[0353] Example 11-1: Results of content determination of the directing agent sec-butylcarbate and chlorantraniliprole in different parts of cotton plants.

[0354] Experimental Methods: The bioactivity of chlorantraniliprole in cotton plants was determined using a pot method. Cotton plants were planted in 15×13×9cm plastic pots, with 4 plants per pot, and cultivated in a greenhouse at 28℃ until the 10-leaf stage. Chlorantraniliprole and tebufenozide were mixed according to the dosages in Table 11-1 and applied as a root drenching treatment to the cotton plants, with 50 mL of the agent per plant. Four replicate plots were set up, with 5 pots in each plot. The content of chlorantraniliprole in the roots, leaves, and stems of the plants treated at different time points was determined by liquid chromatography. The results are shown in Table 11-1.

[0355] Table 11-1 Results of content determination of the directing agents sec-butylcarbate and chlorantraniliprole in different parts of cotton plants

[0356]

[0357]

[0358] Table 11-1 shows that the directing agent sec-butylcarbide can increase the absorption of chlorantraniliprole itself in cotton plants. The addition of sec-butylcarbide significantly increases the systemic absorption of chlorantraniliprole in the roots, stems, and leaves of cotton plants. Among these, the treatments with isoprocarb:chlorantraniliprole ratios of 5:1, 10:1, and 20:1 resulted in the highest systemic absorption of chlorantraniliprole.

[0359] Example 11-2: Determination of the control effect of the combination of the directing agent sec-butylcarbamide and chlorantraniliprole on cotton bollworm.

[0360] Before the pot experiment, a certain amount of sec-butylcarbamide and chlorantraniliprole were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0361] Methods: The systemic effect of the targeted agent tebufenozide on chlorantraniliprole was determined using a pot method. Plastic pots (15×13×9cm) were used, each filled with soil that had passed through a 200-mesh sieve. After cotton plants were kept moist and germinated, they were sown in the plastic pots, 4 seeds per pot. The plants were cultivated in a greenhouse at 28℃ until the cotton plants reached the 10-leaf stage. A fixed amount of chlorantraniliprole and the targeted agent tebufenozide were mixed according to the dosages in Table 11-2. 400mL of the solution was prepared for each treatment. 50mL of the solution was applied to each cotton plant as a root drench. 24 hours later, each cotton plant was inoculated with 2 second-instar bollworms. Four replicates were set up, with 5 pots per replicate. The number of dead bollworms was checked after 48 hours. The pest population reduction rate and control efficacy were calculated, and the results are shown in Table 11-2.

[0362] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0363] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0364] Table 11-2 Control efficacy of methyl parathion and chlorantraniliprole against cotton bollworm

[0365]

[0366]

[0367] As can be seen from Table 12-2, the control efficacy of chlorantraniliprole combined with the directing agent sec-butylcarbide for root drenching significantly increased the control efficacy against cotton bollworm. The highest control efficacy of 88.13% was achieved after 3 days when the ratio of directing agent sec-butylcarbide to chlorantraniliprole was 20:1.

[0368] Example 11-3: Determination of systemic translocation of the directing agent sec-butylcarbate combined with chlorantraniliprole in tomato plant leaves.

[0369] Experimental Methods: A test was conducted on the translocation of chlorantraniliprole and tebufenozide on tomato plants. 100 mg / L chlorantraniliprole and the translocate agent tebufenozide were mixed according to the dosage ratio in Table 12-3, and the mixture was uniformly sprayed onto tomatoes at the 6-leaf stage. Three replicate plots were set up, each with an area of ​​30 m². 2 The content of chlorantraniliprole in the leaves of plants treated at different time points was determined by liquid chromatography. The results are shown in Table 11-3.

[0370] Table 11-3 Results of foliar spraying of tomato plant leaves with a mixture of methylparaben and chlorantraniliprole

[0371]

[0372]

[0373] The results in Table 11-3 indicate that the addition of the directing agent tebuconazole to chlorantraniliprole can increase the systemic absorption of chlorantraniliprole in tomato plants. Among them, the systemic absorption of chlorantraniliprole was highest after 5 days of treatment with tebuconazole:chlorantraniliprole ratios of 5:1, 10:1, and 20:1.

[0374] Example 11-4: Determination of the control effect of the combination of the directing agent tebuconazole and chlorantraniliprole on tomato beet armyworm.

[0375] Before the pot experiment, a certain amount of sec-butylcarbamide and chlorantraniliprole were weighed, dissolved in acetone, and placed in volumetric flasks for later use.

[0376] Test method: The experiment was conducted in fields with severe tomato cutworm infestations. The treatment concentrations were diluted with water and sprayed at a rate of 100 mg / m². 2 Use 40 kg of water and spray the tomatoes evenly on both sides using a standard sprayer. Each treatment was repeated four times, with each plot covering an area of ​​60 m². 2The blocks were randomly arranged among the groups. Before application, the initial insect population was assessed using a 5-point sampling method, with 5 tomato plants sampled at each point, and the insect population count recorded. A follow-up survey was conducted 3 days after application to calculate the insect population reduction rate and control efficacy on affected plants. The results are shown in Table 11-4.

[0377] Insect population reduction rate (%) = (Number of insects before application - Number of insects after application) / Number of insects before application × 100

[0378] Control efficacy (%) = (Pest population reduction rate in the treated area - Pest population reduction rate in the control area) / (100 - Pest population reduction rate in the control area) × 100

[0379] Table 11-4 Field efficacy results of chlorantraniliprole against tomato cutworm.

[0380]

[0381] As can be seen from the data in Table 11-4, the control efficacy of chlorantraniliprole against tomato cutworm was significantly increased after adding the directing agent sec. The highest control efficacy of sec.sec.:chlorantraniliprole = 20:01 was 89.52% after 3 days.

[0382] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An insecticidal composition, characterized in that, The active ingredients are carbamate compounds and amide insecticides in a weight ratio of (1~50):(1~5); among which, The carbamate compound is isopropylcarbate; The amide insecticide mentioned is brofenoxam.

2. The insecticidal composition according to claim 1, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (5~50):(1~5).

3. The insecticidal composition according to claim 1, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~50):(1~2).

4. The insecticidal composition according to claim 1, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~40):(1~2).

5. The insecticidal composition according to claim 1, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (5~20):

1.

6. The insecticidal composition according to claim 1, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~40):

1.

7. The insecticidal composition according to claim 1, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~20):

1.

8. The insecticidal composition according to any one of claims 1 to 7, characterized in that, It also includes other pesticide-acceptable adjuvants selected from one or more of dispersants, wetting agents, emulsifiers, stabilizers, antifreeze agents, defoamers, preservatives, thickeners, organic acids, and dispersion media.

9. The insecticidal composition according to any one of claims 1 to 7, characterized in that, It also includes other pesticide-acceptable adjuvants, which are solvents.

10. The insecticidal composition according to any one of claims 1 to 7, characterized in that, The insecticidal composition is an emulsifiable concentrate, microemulsion, soluble liquid, suspension concentrate, aqueous solution, water emulsion, ultra-low volume liquid, or dispersible oil suspension.

11. The use of the insecticidal composition according to any one of claims 1 to 10 in the control of plant pests; wherein the plant pest is the cotton bollworm or the beet armyworm.

12. The application of carbamate compounds as systemic transdermal agents in amide insecticides, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (1~50):(1~5); The carbamate compound is isopropylcarbate; The amide insecticide mentioned is brofenoxam.

13. The application according to claim 12, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (5~50):(1~5).

14. The application according to claim 12, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~50):(1~2).

15. The application according to claim 12, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~40):(1~2).

16. The application according to claim 12, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (5~20):

1.

17. The application according to claim 12, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~40):

1.

18. The application according to claim 12, characterized in that, The weight ratio of carbamate compounds to amide insecticides is (10~20):

1.

19. A method for improving the utilization rate of amide insecticides, characterized in that, Add carbamate compounds to amide insecticides; The weight ratio of the carbamate compound to the amide insecticide is (1~50):(1~5); The carbamate compound is isopropylcarbate; The amide insecticide mentioned is brofenoxam.

20. The method according to claim 19, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (5~50):(1~5).

21. The method according to claim 19, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (10~50):(1~2).

22. The method according to claim 19, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (10~40):(1~2).

23. The method according to claim 19, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (5~20):

1.

24. The method according to claim 19, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (10~40):

1.

25. The method according to claim 19, characterized in that, The weight ratio of the carbamate compound to the amide insecticide is (10~20):1.

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