A multifunctional intelligent ecological cabin for depriving sleep of mice and rats and a use method thereof

Abstract

The present application relates to a kind of multifunctional intelligent ecological cabin for depriving the sleep of mouse, including bearing box, protective end cap, drive pump, multi-way valve, forced bearing platform and drive circuit, bearing box upper end surface is connected with protective end cap, forced bearing platform is connected with the bottom of bearing box and is vertically distributed, bearing box bottom is equipped with a sewage outlet, lower half of side wall is equipped with a water inlet, sewage outlet, water inlet are all communicated with multi-way valve, multi-way valve is communicated with drive pump, drive pump and drive circuit are all located the outside surface connection of bearing box.Its use method includes three steps of system assembly, preliminary experimental operation and depth experiment.The present application is flexible on one hand experimental means, can effectively meet the needs of various prompting experimental mouse to participate in experiment, and effectively improve the experimental effect and experimental efficiency of experimental mouse sleep deprivation experiment;On the other hand, it effectively simplifies the labor intensity of experimental operation and equipment maintenance operation, while effectively improving the working environment of experimental mouse sleep deprivation experiment.

Description

TECHNICAL FIELD

[0001] The present application relates to biological research equipment, in particular to a multifunctional intelligent ecological cabin for depriving sleep of mice and rats and a use method thereof. BACKGROUND

[0002] The main principle of the mouse and rat sleep deprivation device is to make the mouse and rat unable to enter the sleep state through physical or mental stimulation to achieve the purpose of sleep deprivation. At present, the mouse and rat sleep deprivation devices disclosed mainly include mechanical drum mode, electric shock mode and water environment mode. Compared with the mechanical drum mode and the electric shock mode, the water environment mode is more gentle and easy to accept for the mouse and rat, and therefore is widely used. However, the single water environment has relatively weak interference on the sleep deprivation results of the mouse and rat. Meanwhile, in the water environment mode, the mouse and rat frequently fall into the water tank, so that the hair of the mouse and rat is often in a wet state, which is easy to breed bacteria and cause the body temperature of the experimental mouse to drop, thereby causing the experimental mouse to be sick. In addition, the excrement and food residues of the experimental mouse in the experiment will enter the water tank below, thereby causing the water body to be polluted and the air environment in the experiment to be polluted, further increasing the risk of the experimental mouse being sick, and also causing the workers to frequently replace the sewage and ventilate the air environment in the experiment, which greatly increases the additional workload and working time of the experimenters, and is not conducive to the simultaneous implementation of a large number of experiments and the smooth progress of the experiment.

[0003] In addition, in the current sleep deprivation experiment, due to the lack of effective detection means, the workers often cannot synchronously obtain the physiological parameter change data such as the body temperature and heartbeat of the experimental mouse without affecting the normal experimental state of the experimental mouse, and need to frequently interrupt the experimental conditions of the experimental mouse for independent detection, thereby causing the time result collection to be difficult, the experimental operation to be tedious, the labor intensity to be large, and the detection result accuracy to be relatively low.

[0004] Therefore, in view of the above problems, it is urgent to develop a new experimental mouse sleep deprivation test device and method to meet the actual needs. SUMMARY

[0005] The present application aims to provide a multifunctional intelligent ecological cabin for depriving sleep of mice and rats and a use method thereof. The present application device is integrated and has a high degree of modularization. On the one hand, the experimental means is flexible, which can effectively meet the needs of reminding the experimental mouse to participate in the experiment, and effectively improve the experimental effect and efficiency of the experimental mouse sleep deprivation experiment. On the other hand, the labor intensity of the experimental operation and equipment maintenance work is effectively simplified, and the working environment of the experimental mouse sleep deprivation experiment is effectively improved.

[0006] To achieve the above-mentioned purpose, the present application provides a new multifunctional intelligent ecological cabin for depriving sleep of mice and rats and a use method thereof.

[0007] A multifunctional intelligent ecological cabin for depriving sleep of small and large mice, comprising a bearing box, a protective end cover, a driving pump, a multi-way valve, a forced bearing platform and a driving circuit, the bearing box is a "N" shaped groove structure in cross section, the upper end surface thereof is connected with the protective end cover and forms a closed cavity structure, the forced bearing platform is several, is located in the bearing box and is connected with the bottom of the bearing box and is vertically distributed, and the spacing between each forced bearing platform is at least 1.5 times of the maximum diameter of the upper end surface thereof, and the maximum height of the forced bearing platform is not greater than 80% of the depth of the bearing box, the bottom of the bearing box is provided with a sewage outlet, and the lower half of the side wall is provided with a water inlet, the sewage outlet and the water inlet are communicated with the multi-way valve through the flow guide pipe, the multi-way valve is connected with the outer side surface of the bearing box and is communicated with the driving pump through the flow guide pipe, and the driving pump and the driving circuit are located on the outer side surface of the bearing box, wherein the driving circuit is electrically connected with the driving pump, the multi-way valve and the forced bearing platform, and the forced bearing platforms are connected in parallel.

[0008] Further, the bearing box is provided with an auxiliary support, wherein the auxiliary support is a grid plate mechanism distributed parallel to the outer side surface of the bearing box, and the left side surface and the rear side surface of the bearing box are connected with an auxiliary support through a guide sliding groove, and the upper end surface and the lower end surface of the auxiliary support are at least 5 cm longer than the upper end surface and the lower end surface of the bearing box, respectively, the protective end cover is abutted and slidably connected with the upper end surface of the bearing box, and the outer side surface of the protective end cover is slidably connected with the auxiliary support through a sliding groove, and the two auxiliary supports symmetrically distributed on the left side surface and the right side surface of the bearing box are connected with each other through at least two reinforcing ribs, and the reinforcing ribs are distributed parallel to the bottom of the bearing box, and the two ends thereof are connected with the side surfaces of the auxiliary supports and are vertically distributed, and the reinforcing ribs are evenly distributed along the axis of the bearing box and are abutted and slidably connected with the lower end surface of the bearing box.

[0009] Further, the bearing box is additionally provided with an environmental regulation mechanism, which comprises an electric heating wire, a flow guide air port, a loudspeaker, an irradiation lamp, an ultrasonic transducer, an air exchange fan and a terminal, wherein the electric heating wire is at least one, connected with the inner side of the bearing box and distributed around the axis of the bearing box, the flow guide air port and the irradiation lamp are several, respectively connected with the lower end face of the protective end cover and located in the bearing box, and the axis of the flow guide air port and the irradiation lamp forms an angle of 30°-90° with the bottom of the bearing box, meanwhile the flow guide air port is communicated with the air exchange fan through a flow guide pipe, the air exchange fan is located outside the bottom of the bearing box and connected with the auxiliary support, meanwhile the loudspeaker and the ultrasonic transducer are several, located in the bearing box, connected with the inner side of the bearing box and distributed around the axis of the bearing box, and the loudspeaker and the ultrasonic transducer are distributed at intervals, and the axis of the loudspeaker and the ultrasonic transducer forms an angle of 0°-45° with the bottom of the bearing box, and the axis of each loudspeaker and ultrasonic transducer intersects, and the intersection point is located above the midpoint of the bottom of the bearing box, and the distance between the intersection point and the bottom of the bearing box is not less than 30% of the depth of the bearing box; in addition, the electric heating wire, the loudspeaker, the irradiation lamp, the ultrasonic transducer and the air exchange fan are electrically connected with the terminal through wires, and the terminal is at least one, connected with the auxiliary support and electrically connected with the driving circuit, and the driving circuit is connected with the front end face of the bearing box through an insulating pad.

[0010] Further, the forced bearing table comprises an insulating bearing base, a lifting driving column, a guide column, a main bearing disc, an adjusting bearing disc, an electrode contact, a weighing sensor, a detection electrode and a terminal, wherein the insulating bearing base is a cavity structure with a rectangular axial section, the terminal is embedded in the insulating bearing base and electrically connected with the lifting driving column, the electrode contact, the weighing sensor, the detection electrode and the driving circuit respectively, the guide column is a hollow cylindrical structure coaxially distributed with the insulating bearing base, the lower end face of the guide column is connected with the upper end face of the insulating bearing base through the weighing sensor and vertically distributed, the upper end face of the guide column is connected with the main bearing disc and coaxially distributed, the main bearing disc is a ring structure, the outer diameter of which is at least 2 times of the outer diameter of the guide column, and the inner diameter of which is the same as the inner diameter of the guide column, the lifting driving column is embedded in the guide column, coaxially distributed with the guide column and slidingly connected with the inner side of the guide column, the upper end face of the lifting driving column is connected with the adjusting bearing disc and coaxially distributed, the upper end face of the adjusting bearing disc is located above the upper end face of the main bearing disc by 0 to 5 times of the maximum thickness of the adjusting bearing disc, meanwhile the lower end face of the main bearing disc is provided with at least two electrode contacts which are uniformly distributed around the axis of the main bearing disc, the main bearing disc is provided with assembly holes corresponding to the electrode contacts, and each assembly hole is provided with an electrode contact which is coaxially distributed with the assembly hole, and the upper end face of the electrode contact is flushly distributed with the upper end face of the main bearing disc, the detection electrode is several, uniformly distributed around the axis of the main bearing disc and embedded in the upper end face of the main bearing disc and the adjusting bearing disc respectively.

[0011] Further, the lower end surface of the main bearing disc is parallel to the bottom of the bearing box, and the thickness of the edge of the maximum thickness near the center position is 0-5 cm. The inner side surface of the main bearing disc and the inner side surface of the upper end surface of the guide column are provided with at least one elastic sealing ring, which is connected with the lifting driving column and the adjusting bearing disc outer side surface by sliding.

[0012] Further, the adjusting bearing disc is in any one of spherical cap structure and circular table structure; the lifting driving column is an electric telescopic column, and the upper end surface of the lifting driving column is connected with the lower end surface of the adjusting bearing disc through a rotary table mechanism. The adjusting bearing disc can rotate in the range of 0°-360° through the rotary table mechanism, and the rotary table mechanism is electrically connected with the driving circuit.

[0013] Further, the insulation bearing base is connected with the bearing box through a guide frame, which includes a bearing keel, a connecting sliding groove, a guide sliding rail and an elastic pin. The bearing keel is a frame structure coaxially distributed with the bearing box, and is embedded in the bearing box and abuts against the inner surface of the side wall of the bearing box and the bottom of the bearing box. At least two connecting sliding grooves are arranged on the inner side surface of the bearing keel and symmetrically distributed with the axis of the bearing box. The connecting sliding grooves are located above the bottom of the bearing box by at least 5 mm. The guide sliding rails are embedded in the bearing keel and connected with the inner side surface of the bearing keel through the connecting sliding grooves at both ends. The axis of the guide sliding rail is perpendicular to the axis of the bearing box. The guide sliding rail is connected with the connecting sliding groove through the elastic pin, and is located above the bottom of the bearing box by at least 10 mm. The guide sliding rail is connected with the lower end surface of the insulation bearing base, and the guide sliding rail and the insulation bearing base are connected through the elastic pin.

[0014] Further, the driving circuit is a circuit system based on an FPGA chip, and the driving circuit is further provided with a data communication circuit.

[0015] A method for using a multifunctional intelligent ecological cabin for depriving sleep of mice, comprising the following steps:

[0016] Firstly, the system is assembled. The bearing box, the protective end cover, the driving pump, the multi-way valve, the forced bearing table and the driving circuit are assembled. The number of the forced bearing tables and the working positions between the forced bearing tables are set during the assembly process. After the adjustment of the forced bearing tables is completed, the experimental mice participating in the experiment are placed in the bearing box, and the bearing box is sealed and protected by the protective end cover.

[0017] Second step, preliminary experimental operation, after the first step, the multi-way valve is connected with the external water supply system, then the multi-way valve and the driving pump are driven by the driving circuit to run, the experimental water in the external water supply system is added to the bearing box, and the liquid level in the bearing box is located at 10-50 mm below the main bearing disc of the forced bearing platform. By adding water to the bearing box, the experimental mouse is forced to climb to the main bearing disc and the adjusting bearing disc of the forced bearing platform, so that the experimental mouse is forced to keep awake on the forced bearing platform to prevent falling into water during sleep;

[0018] Third step, deep experiment, after 24 hours of the second step, the adjusting bearing disc is first driven to rise, the height of the upper end surface of the adjusting bearing disc is adjusted to exceed the height of the upper end surface of the main bearing disc, so as to effectively increase the inclination angle of the upper end surface of the forced bearing platform, improve the difficulty of keeping balance of the experimental mouse to prevent falling into water, and force the experimental mouse to keep awake; then the environmental adjusting mechanism of the bearing box is driven to run, on one hand, the air exchange fan and the air guide port cooperate to perform air exchange operation on the air environment in the bearing box; on the other hand, the loudspeaker, the irradiation lamp and the ultrasonic transducer are driven to run, and the experimental mouse is stimulated by sound, light and ultrasonic waves, so as to force the experimental mouse to keep awake, thereby achieving the purpose of the experiment.

[0019] Further, in the third step, when the loudspeaker, the irradiation lamp and the ultrasonic transducer are driven to run, any one of the loudspeaker, the irradiation lamp and the ultrasonic transducer is independently driven to run, or any two of them are driven to run in cooperation, and meanwhile, the three types of devices can be driven to run simultaneously or in an interval type.

[0020] Compared with the prior art, the device has high integration and modularity, on one hand, the experimental means is flexible, can effectively meet the needs of reminding the experimental mouse to participate in the experiment, and effectively improve the experimental effect and efficiency of the sleep deprivation experiment of the experimental mouse, and meanwhile, the physiological function change data of the experimental mouse after sleep deprivation can be synchronously collected during the experiment, and the comprehensiveness of the data is further improved; on the other hand, the labor intensity of experimental operation and equipment maintenance operation is effectively simplified, and meanwhile, the working environment of the sleep deprivation experiment of the experimental mouse is effectively improved, air pollution and the physiological function interference of the experimental mouse caused by the decrease of body temperature due to wet fur in the morning are prevented, and thereby the accuracy of the test result is further improved. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is a partial structure schematic diagram of the present application;

[0022] Figure 2 It is a partial structure schematic diagram of the present application;

[0023] Figure 3 It is a structure schematic diagram of the forced bearing platform in a contracted state;

[0024] Figure 4 Fig. 3 is a structural schematic diagram of the forced bearing table in an unfolded state;

[0025] Figure 5 Fig. 4 is a structural schematic diagram of the main bearing disc;

[0026] Figure 6 Fig. 5 is a schematic diagram of a method for using the present application. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present application will be clearly and completely described with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of the present application.

[0028] Please refer to Figures 1-5 A multifunctional intelligent ecological cabin for depriving sleep of mice and rats comprises a bearing box 1, a protective end cover 2, a driving pump 3, a multi-way valve 4, forced bearing tables 5, and a driving circuit 6. The bearing box 1 has a "N" shaped groove structure in the cross section. The upper end surface of the bearing box 1 is connected with the protective end cover 2 and forms a closed cavity structure. The forced bearing tables 5 are located in the bearing box 1 and are connected with the bottom of the bearing box 1 and are distributed vertically. The spacing between the forced bearing tables 5 is at least 1.5 times the maximum diameter of the upper end surface of the forced bearing tables 5. The maximum height of the forced bearing tables 5 is not greater than 80% of the depth of the bearing box 1. A sewage outlet 7 is arranged at the bottom of the bearing box 1, and a water inlet 8 is arranged at the lower half of the side wall. The sewage outlet 7 and the water inlet 8 are communicated with the multi-way valve 4 through flow guide pipes. The multi-way valve 4 is connected with the outer side surface of the bearing box 1 and is communicated with the driving pump 3 through flow guide pipes. The driving pump 3 and the driving circuit 6 are located on the outer side surface of the bearing box 1. The driving circuit 6 is electrically connected with the driving pump 3, the multi-way valve 4, and the forced bearing tables 5, respectively. The forced bearing tables 5 are connected in parallel with each other.

[0029] In the embodiment, the bearing box 1 is additionally provided with an auxiliary support 9, wherein the auxiliary support 9 is a grid plate mechanism distributed in parallel with the outer side of the bearing box 1, and the left side and the rear side of the bearing box 1 are respectively connected with an auxiliary support 9 through a guide sliding groove 10, while the upper end surface and the lower end surface of the auxiliary support 9 respectively exceed the upper end surface and the lower end surface of the bearing box 1 by at least 5 cm, the protective end cover 2 is abutted and slidably connected with the upper end surface of the bearing box 1, while the outer side of the protective end cover 2 is additionally slidably connected with the auxiliary support 9 through a sliding groove 11, while the two auxiliary supports 9 symmetrically distributed on the left side and the right side of the bearing box 1 are additionally connected with each other through at least two reinforcing ribs 12, and the reinforcing ribs 12 are distributed in parallel with the bottom of the bearing box 1, and the two ends thereof are respectively connected with the side surfaces of the auxiliary supports 9 and are distributed vertically, while each reinforcing rib 12 is distributed along the axial direction of the bearing box 1, and is abutted and slidably connected with the lower end surface of the bearing box 1.

[0030] By setting the auxiliary support, the flexibility and convenience of the installation and assembly operation between the bearing box and the external equipment can be effectively improved, while the flexibility of the auxiliary equipment installed outside the bearing box is also improved, and the friction loss and collision failure of the bearing box caused by the direct contact between the bearing box and the external equipment are prevented.

[0031] Particularly, the bearing box 1 is additionally provided with an environmental adjustment mechanism 13, which comprises an electric heating wire 131, a flow guide air port 132, a loudspeaker 133, an irradiation lamp 134, an ultrasonic transducer 135, an air exchange fan 136 and a wiring terminal 137, wherein the electric heating wire 131 is at least one, connected with the inner side of the bearing box 1 and distributed around the axis of the bearing box 1, the flow guide air port 132 and the irradiation lamp 134 are both several, respectively connected with the lower end surface of the protective end cover 2 and located in the bearing box 1, and the axis of the flow guide air port 132 and the irradiation lamp 134 forms an angle of 30°-90° with the bottom of the bearing box 1, meanwhile, each flow guide air port 132 is communicated with the air exchange fan 136 through a flow guide pipe, the air exchange fan 136 is located outside the bottom of the bearing box 1 and connected with the auxiliary support 9, meanwhile, the loudspeaker 133 and the ultrasonic transducer 135 are both several, located in the bearing box 1, connected with the inner side of the bearing box 1 and distributed around the axis of the bearing box 1, meanwhile, the loudspeaker 133 and the ultrasonic transducer 135 are distributed at intervals, and the axis of the loudspeaker 133 and the ultrasonic transducer 135 forms an angle of 0°-45° with the bottom of the bearing box 1, and the axis of each loudspeaker 133 and ultrasonic transducer 135 intersects, and the intersection point is located above the midpoint of the bottom of the bearing box 1, and the distance between the intersection point and the bottom of the bearing box 1 is not less than 30% of the depth of the bearing box 1; in addition, the electric heating wire 131, the loudspeaker 133, the irradiation lamp 134, the ultrasonic transducer 135 and the air exchange fan 136 are electrically connected with the wiring terminal 137 through wires, and the wiring terminal 137 is at least one, connected with the auxiliary support 9 and electrically connected with the driving circuit 6, and the driving circuit 6 is connected with the front end surface of the bearing box 1 through an insulating pad.

[0032] Meanwhile, the experimental temperature environment in the bearing box can be effectively adjusted by the electric heating wire, and the air in the bearing box can be exchanged by the flow guide air port and the air exchange fan, so as to effectively reduce the pollution caused by the irritating gas in the bearing box during the experiment due to the experimental mouse feed and excrement.

[0033] In addition, the experimental mouse after falling into the water can be dried by the cooperation of the air flow and the electric heating wire, so as to prevent the physiological function of the experimental mouse from being reduced due to the wet fur of the experimental mouse, thereby affecting the accuracy of the test result.

[0034] In addition, in the experiment, the experimental mouse can be stimulated by noise, strong light and ultrasonic means through the loudspeaker, the irradiation lamp and the ultrasonic transducer, so as to force the experimental mouse to keep awake, thereby improving the working efficiency of the experiment and the flexibility of the experimental means.

[0035] The force bearing platform 5 includes an insulating bearing base 51, a lifting drive column 52, a guide column 53, a main bearing disc 54, an adjusting bearing disc 55, an electrode contact 56, a weighing sensor 58, a detection electrode 59, and a wiring terminal 137. The insulating bearing base 51 is a cavity structure with a rectangular axial section. The wiring terminal 137 is embedded in the insulating bearing base 51 and electrically connected with the lifting drive column 52, the electrode contact 56, and the driving circuit 6. The guide column 53 is a hollow cylindrical structure coaxially distributed with the insulating bearing base 51. The lower end surface of the guide column 53 is connected with the upper end surface of the insulating bearing base 51 through the weighing sensor 58 and vertically distributed. The upper end surface of the guide column 53 is connected with the main bearing disc 54 and coaxially distributed. The main bearing disc 54 is a ring structure with an outer diameter at least 2 times the outer diameter of the guide column 53 and an inner diameter same as the inner diameter of the guide column 53. The lifting drive column 52 is embedded in the guide column 53, coaxially distributed with the guide column 53, and slidingly connected with the inner side surface of the guide column 53. The upper end surface of the lifting drive column 52 is connected with the adjusting bearing disc 55 and coaxially distributed. The upper end surface of the adjusting bearing disc 55 is located above the upper end surface of the main bearing disc 54 by 0 to 5 times the maximum thickness of the adjusting bearing disc 55. At least two electrode contacts 56 are arranged around the axis of the main bearing disc 54. The main bearing disc 54 is provided with assembly holes 57 corresponding to the electrode contacts 56. Each assembly hole 57 is provided with an electrode contact 56 coaxially distributed therein. The upper end surfaces of the electrode contacts 56 are flushly distributed with the upper end surface of the main bearing disc 54. The detection electrode 59 is evenly arranged around the axis of the main bearing disc 54 and embedded in the upper end surfaces of the main bearing disc 54 and the adjusting bearing disc 55.

[0036] Further optimization, the lower end surface of the main bearing disc is parallelly distributed with the bottom of the bearing box. The maximum thickness of the edge near the center of the main bearing disc is 0-5 cm. At least one elastic sealing ring 16 is arranged on the inner side surface of the main bearing disc and the upper end surface of the guide column. The elastic sealing ring 16 is in abutment and sliding connection with the outer side surface of the lifting drive column 52 and the adjusting bearing disc 55.

[0037] Meanwhile, the adjusting bearing disc is in any one of a spherical cap structure and a circular table structure. The lifting drive column is an electric telescopic column. The upper end surface of the lifting drive column 52 is connected with the lower end surface of the adjusting bearing disc 5 through a rotary table mechanism 15. The adjusting bearing disc can rotate in a range of 0°-360° through the rotary table mechanism 15. The rotary table mechanism is electrically connected with the driving circuit.

[0038] Further optimization, the detection electrode includes but is not limited to any one or several of a temperature sensor, a blood oxygen sensor, a heart rate sensor, and a bioelectric impedance sensor.

[0039] In the experiment, on the one hand, the working height of the adjusting bearing disc can be driven by the lifting drive column, and the spherical crown structure of the adjusting bearing disc is adjusted, so that the surface of the upper end face of the forced bearing platform for the experimental mouse to climb is formed into an inclined surface by making the adjusting bearing disc higher than the upper end face of the main bearing disc, the climbing difficulty of the experimental mouse is increased, and the purpose of forcing the experimental mouse to keep awake is achieved.

[0040] On the other hand, in the operation, the experimental mouse can be stimulated by the electrode contact to achieve the purpose of forcing the experimental mouse to keep awake.

[0041] In addition, the adjusting bearing disc can be driven to rotate by the rotating table mechanism, and the difficulty of the experimental mouse in climbing the forced bearing platform is further improved.

[0042] In addition, in the experiment, on the one hand, the weight of the experimental mouse can be effectively detected by the weighing sensor; on the other hand, the physiological parameters such as body temperature and heartbeat of the experimental mouse can be detected in the detection process by the detection electrode.

[0043] In addition, the insulating bearing base 51 is slidably connected between the bearing box 1 and the guide frame 14, the guide frame 14 includes a bearing keel 141, a connecting sliding groove 142, a guide sliding rail 143, and an elastic pin 144, wherein the bearing keel 141 is a frame structure coaxially distributed with the bearing box 1, the bearing keel 141 is embedded in the bearing box 1 and abuts against the inner surface of the side wall of the bearing box 1 and the bottom of the bearing box 1, at least two connecting sliding grooves 142 are arranged on the inner side of the bearing keel 141 and symmetrically distributed with the axis of the bearing box 141, the connecting sliding groove 142 is located above the bottom of the bearing box 1 by at least 5 mm, the guide sliding rail 143 is embedded in the bearing keel 141 and slidably connected with the inner side of the bearing keel 141 at both ends of the guide sliding rail 143 through the connecting sliding groove 142, the axis of the guide sliding rail 143 is perpendicular to the axis of the bearing box 1, the guide sliding rail 143 is connected with the connecting sliding groove 142 through the elastic pin 144, and the guide sliding rail 143 is located above the bottom of the bearing box 1 by at least 10 mm, the guide sliding rail 143 is slidably connected with the lower end surface of the plurality of insulating bearing bases 51, and the guide sliding rail 143 and the insulating bearing base 51 are connected through the elastic pin 144.

[0044] In this embodiment, the driving circuit 6 is a circuit system based on an FPGA chip, and the driving circuit 6 further has a data communication circuit.

[0045] As shown in Figure 6 A use method of a multifunctional intelligent ecological cabin for depriving sleep of rats and mice, comprising the following steps:

[0046] The first step is system assembly, first, the bearing box, protective end cover, drive pump, multi-way valve, forced bearing platform and drive circuit are assembled, and the number of forced bearing platforms and the working positions between the forced bearing platforms are set during the assembly process, and after the adjustment of the forced bearing platforms is completed, the experimental mice participating in the experiment are placed in the bearing box, and the bearing box is sealed and protected by the protective end cover;

[0047] The second step is the preliminary experimental operation, after the first step is completed, the multi-way valve is connected with the external water supply system, then the drive circuit drives the multi-way valve and the drive pump to run at the same time, the experimental water in the external water supply system is added to the bearing box, and the liquid level in the bearing box is located at 10-50 mm below the main bearing disc of the forced bearing platform, by adding water to the bearing box, the experimental mice are forced to climb to the main bearing disc and the adjusting bearing disc of the forced bearing platform, so that the experimental mice are forced to keep awake on the forced bearing platform to prevent falling into water during sleep;

[0048] The third step is the depth experiment, after 24 hours of the preliminary experimental operation of the second step, first, the lifting drive column of the forced bearing platform is driven to run, the adjusting bearing disc is raised, and the height of the upper end surface of the adjusting bearing disc is adjusted to exceed the height of the upper end surface of the main bearing disc, so as to effectively increase the inclination angle of the upper end surface of the forced bearing platform, improve the difficulty of the experimental mice to keep balance and prevent falling into water, and force the experimental mice to keep awake; then the environmental adjusting mechanism of the bearing box is driven to run, on the one hand, the air exchange fan and the air guide air outlet cooperate to perform air exchange operation on the air environment in the bearing box; on the other hand, the loudspeaker, the irradiation lamp and the ultrasonic transducer are driven to run, and the experimental mice are stimulated by sound, light and ultrasonic waves, so as to force the experimental mice to keep awake, thereby achieving the purpose of the experiment.

[0049] In the third step of the embodiment, when the loudspeaker, the irradiation lamp and the ultrasonic transducer are driven to run, any one of the loudspeaker, the irradiation lamp and the ultrasonic transducer is independently run or any two of them are cooperatively run, and simultaneously, the three types of equipment can be simultaneously run or interval type cyclically run.

[0050] Compared with the prior art, the equipment of the present application has high integration and modularity, on the one hand, the experimental means is flexible, which can effectively meet the needs of various experimental mice to participate in the experiment, and effectively improve the experimental effect and efficiency of the sleep deprivation experiment of the experimental mice; on the other hand, the labor intensity of the experimental operation and equipment maintenance operation is effectively simplified, and the working environment of the sleep deprivation experiment of the experimental mice is effectively improved.

[0051] It is to be noted that, in the present text, the relative terms such as first and second, and the like, are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between such entities or operations. Moreover, the terms "comprising", "including", or any other variation thereof are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can also include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the statement "comprising a" does not exclude the existence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0052] In the description of the present specification, the terms "connection", "installation", "fixation", "arrangement", and the like are to be understood in a broad sense, for example, "connection" can be a fixed connection or indirectly through an intermediate component without affecting the relationship and technical effects of the components, or can be an integral connection or partial connection, as the case may be for a person of ordinary skill in the art, the specific meaning of the above terms in the present invention or invention can be understood according to the specific circumstances.

[0053] Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multifunctional intelligent ecological cabin for depriving sleep of small rodents, characterized in that, The multifunctional intelligent ecological cabin for depriving sleep of mice and rats comprises a bearing box, a protective end cover, a driving pump, a multi-way valve, forced bearing tables and a driving circuit, the bearing box is a "N" shaped groove structure in cross section, the upper end surface of the bearing box is connected with the protective end cover and forms a closed cavity structure, the forced bearing tables are arranged in the bearing box and are connected with the bottom of the bearing box and are vertically distributed, the spacing between the forced bearing tables is at least 1.5 times of the maximum diameter of the upper end surface of the forced bearing tables, and the maximum height of the forced bearing tables is not greater than 80% of the depth of the bearing box, a sewage outlet is arranged at the bottom of the bearing box, a water inlet is arranged at the lower half of the side wall of the bearing box, the sewage outlet and the water inlet are communicated with the multi-way valve through flow guide pipes, the multi-way valve is connected with the outer side surface of the bearing box and is communicated with the driving pump through the flow guide pipes, and the driving pump and the driving circuit are arranged on the outer side surface of the bearing box, wherein the driving circuit is electrically connected with the driving pump, the multi-way valve and the forced bearing tables respectively, and the forced bearing tables are connected in parallel with each other; The forced bearing table comprises an insulating bearing base, a lifting driving column, a guide column, a main bearing disc, an adjusting bearing disc, an electrode contact, a weighing sensor, a detection electrode and a wiring terminal, wherein the insulating bearing base is a cavity structure in axial section, the wiring terminal is embedded in the insulating bearing base and is electrically connected with the lifting driving column, the electrode contact, the weighing sensor, the detection electrode and the driving circuit respectively, the guide column is a hollow cylindrical structure coaxially distributed with the insulating bearing base, the lower end surface of the guide column is connected with the upper end surface of the insulating bearing base through the weighing sensor and is vertically distributed, the upper end surface of the guide column is connected with the main bearing disc and is coaxially distributed, the main bearing disc is a ring structure, the outer diameter of the main bearing disc is at least 2 times of the outer diameter of the guide column, and the inner diameter of the main bearing disc is the same as the inner diameter of the guide column, the lifting driving column is embedded in the guide column, is coaxially distributed with the guide column and is slidingly connected with the inner side surface of the guide column, the upper end surface of the lifting driving column is connected with the adjusting bearing disc and is coaxially distributed, the upper end surface of the adjusting bearing disc is located above the upper end surface of the main bearing disc by 0 to 5 times of the maximum thickness of the adjusting bearing disc, at least two electrode contacts are arranged on the lower end surface of the main bearing disc and are uniformly distributed around the axis of the main bearing disc, the main bearing disc is provided with assembly holes corresponding to the electrode contacts, each assembly hole is provided with an electrode contact coaxially distributed therein, and the upper end surfaces of the electrode contacts are flushly distributed with the upper end surface of the main bearing disc, and the detection electrodes are uniformly distributed around the axis of the main bearing disc and are embedded in the upper end surfaces of the main bearing disc and the adjusting bearing disc.

2. The multifunctional intelligent ecological cabin for depriving sleep of mice according to claim 1, characterized in that, The auxiliary support is a grid plate mechanism distributed in parallel with the outer side of the bearing box, and the left side and the rear side of the bearing box are connected with an auxiliary support through a guide chute, respectively, and the upper end surface and the lower end surface of the auxiliary support are at least 5 cm longer than the upper end surface and the lower end surface of the bearing box, respectively. The protective end cover is abutted and slidably connected with the upper end surface of the bearing box, and the outer side of the protective end cover is slidably connected with the auxiliary support through a sliding groove. The two auxiliary supports symmetrically distributed on the left side and the right side of the bearing box are connected with each other through at least two reinforcing ribs, and the reinforcing ribs are distributed in parallel with the bottom of the bearing box, and the two ends of the reinforcing ribs are connected with the side surfaces of the auxiliary supports and are distributed vertically, and each reinforcing rib is uniformly distributed along the axial direction of the bearing box and is abutted and slidably connected with the lower end surface of the bearing box.

3. The multifunctional intelligent ecological cabin for depriving sleep of mice according to claim 2, characterized in that, The bearing box is additionally provided with an environment adjusting mechanism, and the environment adjusting mechanism comprises an electric heating wire, a flow guide air port, a loudspeaker, an irradiation lamp, an ultrasonic transducer, a ventilation fan and a terminal. The electric heating wire is at least one, connected with the inner side of the bearing box and distributed around the axis of the bearing box. The flow guide air port and the irradiation lamp are several, connected with the lower end surface of the protective end cover and located in the bearing box, and the axes of the flow guide air port and the irradiation lamp form an angle of 30°-90° with the bottom of the bearing box. Each flow guide air port is communicated with the ventilation fan through a flow guide pipe. The ventilation fan is located outside the bottom of the bearing box and connected with the auxiliary support. The loudspeaker and the ultrasonic transducer are several, located in the bearing box, connected with the inner side of the bearing box and uniformly distributed around the axis of the bearing box. The loudspeaker and the ultrasonic transducer are distributed at intervals, and the axes of the loudspeaker and the ultrasonic transducer form an angle of 0°-45° with the bottom of the bearing box. The axes of the loudspeaker and the ultrasonic transducer intersect, and the intersection point is located above the midpoint of the bottom of the bearing box. The distance between the intersection point and the bottom of the bearing box is not less than 30% of the depth of the bearing box. In addition, the electric heating wire, the loudspeaker, the irradiation lamp, the ultrasonic transducer and the ventilation fan are electrically connected with the terminal through wires. The terminal is at least one, connected with the auxiliary support and electrically connected with the driving circuit. The driving circuit is connected with the front end surface of the bearing box through an insulating pad.

4. The multifunctional intelligent ecological cabin for depriving sleep of mice according to claim 3, characterized in that, The lower end surface of the main bearing disc is distributed in parallel with the bottom of the bearing box, and the maximum thickness near the center position is 0-5 cm larger than the thickness at the edge. The inner side of the main bearing disc and the inner side of the upper end surface of the guide column are provided with at least one elastic sealing ring, which is abutted and slidably connected with the outer side of the lifting driving column and the adjusting bearing disc through the elastic sealing ring.

5. The multifunctional intelligent ecological cabin for depriving sleep of mice according to claim 4, characterized in that, The adjusting bearing disc is in any one of spherical cap structure and circular table structure. The lifting driving column is an electric telescopic column, and the upper end surface of the lifting driving column is connected with the lower end surface of the adjusting bearing disc through a rotary table mechanism. The adjusting bearing disc can rotate in the range of 0°-360° through the rotary table mechanism, and the rotary table mechanism is electrically connected with the driving circuit.

6. The multifunctional intelligent ecological cabin for depriving sleep of mice according to claim 5, characterized in that, The insulation bearing base is slidably connected with the bearing box through a guide frame, the guide frame comprises a bearing keel, a connecting sliding groove, a guide sliding rail and an elastic pin, wherein the bearing keel is a frame structure coaxially distributed with the bearing box, the bearing keel is embedded in the bearing box and abuts against the inner surface of the side wall of the bearing box and the bottom of the bearing box, at least two connecting sliding grooves symmetrically distributed with the axis of the bearing box are arranged on the inner side of the bearing keel, the connecting sliding grooves are located above the bottom of the bearing box by at least 5 mm, the guide sliding rails are embedded in the bearing keel and slidably connected with the inner side of the bearing keel through the connecting sliding grooves at both ends of the guide sliding rails, the axis of the guide sliding rail is vertically distributed with the axis of the bearing box, the guide sliding rail is connected with the connecting sliding groove through the elastic pin, and the guide sliding rail is located above the bottom of the bearing box by at least 10 mm, the guide sliding rail is slidably connected with the lower end surface of the plurality of insulation bearing bases, and the guide sliding rail and the insulation bearing base are connected through the elastic pin.

7. The multifunctional intelligent ecological cabin for depriving sleep of mice according to claim 6, characterized in that, The driving circuit is a circuit system based on an FPGA chip, and the driving circuit is additionally provided with a data communication circuit.

8. The method of claim 7, wherein the multi-functional intelligent ecological cabin for depriving sleep of mice is characterized in that, The use method of the multifunctional intelligent ecological cabin for depriving sleep of mice and rats comprises the following steps: First step, system assembly, firstly, the bearing box, the protective end cover, the driving pump, the multi-way valve, the forced bearing table and the driving circuit are assembled, and the number of the forced bearing tables and the working positions between the forced bearing tables are set during the assembly process, and after the adjustment of the forced bearing tables is completed, the experimental mice participating in the experiment are placed in the bearing box, and the bearing box is sealed and protected by the protective end cover; Second step, preliminary experimental operation, after the first step is completed, the multi-way valve is connected with the external water supply system, then the driving circuit is used to drive the multi-way valve and the driving pump to operate at the same time, the experimental water in the external water supply system is added into the bearing box, and the liquid level in the bearing box is located at 10-50 mm below the main bearing disc of the forced bearing table, by adding water into the bearing box, the experimental mice are forced to climb to the main bearing disc and the adjusting bearing disc of the forced bearing table, so that the experimental mice are forced to keep awake on the forced bearing table to prevent falling into water during sleep; Third step, deep experiment, after 24 hours of the preliminary experimental operation of the second step, firstly, the lifting driving column of the forced bearing table is driven to operate, so that the adjusting bearing disc is raised, the height of the upper end surface of the adjusting bearing disc is adjusted to exceed the upper end surface of the main bearing disc, thereby effectively increasing the inclination angle of the upper end surface of the forced bearing table, improving the difficulty of the experimental mice to keep balance and prevent falling into water, and forcing the experimental mice to keep awake; then, the environmental adjusting mechanism arranged on the bearing box is driven to operate, on one hand, the air exchange fan and the air guide air outlet cooperate to perform air exchange operation on the air environment in the bearing box; on the other hand, the loudspeaker, the irradiation lamp and the ultrasonic transducer are driven to operate, and the experimental mice are stimulated by sound, light and ultrasonic waves, so that the experimental mice keep awake, thereby achieving the purpose of the experiment.

9. The use of a multifunctional intelligent ecological cabin for depriving sleep of mice and rats according to claim 8, characterized in that, In the third step, when the loudspeaker, the irradiation lamp and the ultrasonic transducer are driven to operate, any one of the loudspeaker, the irradiation lamp and the ultrasonic transducer is independently operated, or any two of them are cooperatively operated, or the three types of devices are simultaneously operated or are cyclically operated in an interval mode.

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