A pollution-free cultivation method for king oyster mushroom

Abstract

The present invention discloses a pollution-free cultivation method for King Oyster Mushroom, specifically relating to the technical field of King Oyster Mushroom cultivation, comprising the following specific steps: S1, selecting a culture environment, S2, preparing a culture medium, S3, filling the culture medium, S4, adding culture nutrient materials, S5, soaking and steaming, S6, inoculating the strain, S7, piling up the culture medium, S8, regulating culture conditions, adding water to the culture medium to maintain humidity, and S9, picking and harvesting. The present invention places the culture nutrient materials into an extruder, and a mobile injection machine injects the culture nutrient materials into a central hole. The mobile injection machine moves the culture nutrient materials to the circular hole for injection, ensuring that a specified amount of culture nutrient materials is provided at multiple layers of the culture medium from the outside to the inside. Later, no more spraying of an influencing liquid is required to provide the nutrients required for the growth of King Oyster Mushroom, thereby reducing the cultivation cost and increasing the growth rate of King Oyster Mushroom.

Description

Technical Field

[0001] The present invention relates to the technical field of King Oyster Mushroom cultivation, and more particularly to a pollution-free King Oyster Mushroom cultivation method. Background Art

[0002] King Oyster Mushroom is a typical wild edible fungus in the subtropical grassland and arid desert areas. It is saprophytic and facultatively parasitic on the roots and surrounding soil of large Umbelliferae plants such as Eryngium, Ferula, and Lassia. In the cultivation process of King Oyster Mushroom, the main method used is pollution-free cultivation.

[0003] Among the existing published documents, patent publication number CN106852255A discloses a method for cultivating King Oyster Mushroom. In response to the currently commonly used high-temperature steam sterilization method, which causes the culture medium to collapse and clump easily during the sterilization process, resulting in poor air permeability, affecting the uniform inoculation of subsequent strains, and further affecting the uniformity and consistency of mushroom production, resulting in uneven quality of King Oyster Mushrooms and affecting economic benefits, the culture medium is subjected to a certain degree of anti-gravity flushing, which ensures that the culture medium will not collapse and air permeability is guaranteed, which is conducive to the uniform inoculation of strains, good consistency of bacterial age, and neat mushroom production. Both ports of the improved culture bottle can be inoculated with strains, that is, within the same cultivation cycle, the amount of mushrooms produced has doubled, improving economic benefits; however, the King Oyster Mushroom cultivation method still has the following defects;

[0004] During cultivation, the above-mentioned King Oyster Mushroom grows directly from the outside into the culture medium. Since it is difficult for the culture medium to have the required nutrients, a nutrient solution needs to be provided from the outside. This requires adding a large amount of nutrient solution for watering and spraying in the later stage to increase the nutrients required for the growth of the King Oyster Mushroom. Not only is it difficult for the nutrient solution to penetrate into the culture medium, but the nutrient solution needs to be sprayed by equipment and machines, resulting in certain losses. This not only increases the cultivation cost but also reduces the growth rate of the King Oyster Mushroom. Therefore, it is necessary to provide a pollution-free cultivation method for King Oyster Mushroom. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a pollution-free cultivation method of Pleurotus eryngii.

[0006] To achieve the above object, the present invention provides the following technical solution: a pollution-free cultivation method of King Oyster Mushroom, comprising the following specific steps:

[0007] S1. Cultivation environment selection: Choose a place with good ventilation, suitable temperature and good humidity control for cultivation. The temperature range for the growth of King Oyster Mushroom is between 20-25℃ and the relative humidity is maintained at 80%-90%;

[0008] S2. Culture medium preparation: The culture medium uses 100 parts of corn stalks, 50 parts of rice straw, and 30-50 parts of sawdust as the main raw materials. The raw materials are cut into 5-10 cm lengths and then steamed for 30 to 60 minutes to eliminate bacteria and spores. 5-10 parts of nitrate nitrogen and 5-10 parts of ammonium nitrogen, 5-10 parts of calcium salt powder, 8-10 parts of sucrose, 5-10 parts of inositol, 5-10 parts of organic additives, and 5-15 parts of growth regulators are added and soaked for 40-60 minutes. After steaming, the culture medium is cooled to an appropriate temperature.

[0009] S3. Filling the culture medium: Using a cutting tool, a circular hole with a diameter of 10-20 cm is opened at the center of the culture medium. A water-permeable filter membrane is placed inside the circular hole. Multiple middle holes with a diameter of 10-20 cm are opened along the top of the culture medium. A water-permeable filter membrane is placed in the middle hole. Edge holes with a diameter of 5-10 cm are opened at the edge of the culture medium. A water-permeable filter membrane is placed in the edge holes.

[0010] S4. Adding culture nutrient raw materials: The culture nutrient raw materials are placed into the extruder, injected into the edge holes by the injection machine, and then injected into the middle holes by the mobile injection machine. The mobile injection machine moves the culture nutrient raw materials to the dot holes for injection. After the injection is completed, the compactor is used to perform vertical compaction;

[0011] S5. Soaking and cooking: Place the compacted culture medium in a cooking chamber, heat it to 50-60°C and keep it warm for 5-10 minutes, then heat it to 80-100°C and keep it warm for 3-5 minutes. After cooling to room temperature, soak it in an appropriate amount of potassium iodate and sufficient deionized water for 5-10 minutes.

[0012] S6. Inoculation: Place the culture medium to be inoculated into a petri dish, then pour in a culture medium cooled to 45°C. Evenly spread the King Oyster Mushroom spawn on the culture medium at an inoculum rate of 200-300g per 100kg of culture medium. Quickly and gently shake the culture medium until the spawn is diluted and evenly covers the culture medium. Cover the culture medium with a plastic mesh bag and place the culture medium in a designated location. Excess spawn is removed by rotating the culture medium.

[0013] S7. Culture medium stacking: Stack the inoculated culture medium in a container with each layer no thicker than 15 cm. Use a bracket to support the outer wall of each culture medium, reinforce the culture medium and maintain the relative humidity at 80%-90%, and the temperature control range is between 20-25°C.

[0014] S8. Culture condition control: Add water to the culture medium to maintain humidity and spray water regularly to moisten the mycelium. At the same time, maintain the temperature control range between 20-25℃ and the relative humidity between 70% and 80%. Pay attention to ventilation during the growth period. Check the water level every day to avoid excessive evaporation and dryness. Spray water in time to maintain the relative humidity between 80% and 90%;

[0015] S9. Harvesting: When the King Oyster Mushrooms are full and the caps begin to open, they can be picked. Rotate the entire fruiting body and cut it off with scissors.

[0016] Preferably, the temperature and humidity information of the King Oyster Mushroom growth environment collected in S1 is sent to the central processing unit for processing. The central processing unit judges and compares the collected temperature and humidity information to determine whether the temperature and humidity in the King Oyster Mushroom growth environment are within the set range. If not within the specified range, humidity and temperature control are required, and the temperature and humidity need to be measured once every 30 minutes using a temperature and humidity sensor.

[0017] Preferably, the growth regulator in S2 is prepared by adding 5-10 parts of zeatin and 5-10 parts of 6-benzylpurine into a stirring tank, controlling the speed of the stirring tank to 40-60 r / min, stirring for 5-15 minutes, heating the stirring tank to 50-80°C, keeping warm for 3-5 minutes, cooling to room temperature, adding 2-5 parts of gibberellic acid and soaking for 5-10 minutes, wearing gloves after soaking, taking out the growth regulator and placing it in a holding bottle, using a vacuum machine to remove the air in the holding bottle for backup use, and preparing the organic additive in S2 by putting 5-10 parts of casein hydrolyzate, 1-5 parts of yeast extract, and 5-20 parts of coconut juice into a mixer, mixing at a speed of 10-20 r / min for 5-10 minutes, heating the mixer to 40°C, keeping warm for 3-5 minutes, then heating to 50-80°C, adding 1-3 parts of corn starch, and mixing at a speed of 10-25 r / min for 3-10 minutes.

[0018] Preferably, the number of dot holes in the S3 is set to 1, the number of middle holes is set to 10-15, the number of edge holes is set to 15-20, and multiple edge holes are arranged in a circular ring with equal spacing. The S3 water-permeable filter membrane is mainly prepared by mixing 1-5 parts of tert-butyl peroxyisopropylbenzene, 3-10 parts of fumaric acid, 5-10 parts of microcrystalline wax, and 2-10 parts of polyethylene wax, and then injection-molded by an injection molding machine. A circular through hole with a cross-sectional shape of 5-10 mm needs to be opened on the water-permeable filter membrane.

[0019] Preferably, the culture nutrient raw materials in S4 are formed by stirring 15-25 parts of potatoes, 2-5 parts of glucose, 1-5 parts of peptone, 0.5-1 part of aluminum dihydrogen phosphate, 0.2-1 part of magnesium sulfate, and 5-10 parts of agar powder. The injection pressure of the injection machine in S4 is 1-1.5Mpa, and the injection time is 20-30S. The injection machine port needs to be wiped after each injection, and 3-5 injection personnel are required to supervise each other to see whether the culture nutrient raw materials in the dot holes, edge holes, and middle holes are full.

[0020] Preferably, the culture medium in S6 needs to be rotated at a speed of 10-20 r / min, and the bacterial liquid needs to be discharged for 5-10 seconds. The discharge personnel need to wear goggles.

[0021] The technical effects and advantages of the present invention are as follows:

[0022] 1. The present invention puts culture nutrient raw materials into an extruder. The culture nutrient raw materials are formed by stirring potatoes, glucose, peptone, aluminum dihydrogen phosphate, magnesium sulfate, and agar powder. The culture nutrient raw materials are injected into the edge holes by an injection machine. The mobile injection machine injects the culture nutrient raw materials into the middle hole. The mobile injection machine moves the culture nutrient raw materials to the dot holes for injection. The dot holes, edge holes, and middle holes are supervised to see whether the culture nutrient raw materials are filled. This ensures that the culture nutrient raw materials of a specified amount are provided at multiple positions from the outside to the inside of the culture medium. In the later stage, the nutrients required for the growth of King Oyster Mushroom can be provided without spraying the influencing liquid. The nutrients are provided from the inside of the culture medium, which avoids reducing the loss of the nutrient solution spraying, not only reducing the cultivation cost but also improving the growth rate of King Oyster Mushroom.

[0023] 2. The present invention provides corn stalks, rice straw, and sawdust as main raw materials, which are cut into specified lengths and then steamed to eliminate bacteria and spores. Nitrate nitrogen and ammonium nitrogen, calcium salt powder, sucrose, inositol, organic additives, and growth regulators are added to provide King Oyster Mushroom with a variety of basic nutrients required for growth, thereby increasing the growth rate of King Oyster Mushroom and achieving pollution-free cultivation after steaming.

[0024] 3. The oyster mushroom spawn of the present invention is poured into a cooled culture medium, and the oyster mushroom spawn is evenly spread on the culture medium. The culture medium is inoculated with the amount of bacteria used, and the culture medium is quickly and gently shaken to dilute the bacterial solution and evenly cover the culture medium. A plastic mesh bag is put on the culture medium, and the culture medium is placed in a designated position. The excess bacterial solution is removed by rotating the culture medium. The rotation speed of the culture medium needs to be maintained during rotation, and the bacterial solution is removed for a certain period of time to ensure that each culture medium can make the oyster mushroom spawn evenly distributed and contact the bacterial solution, so that the cultivated oyster mushroom spawn is more evenly distributed, and the growth rate of the oyster mushroom cultivation is improved;

[0025] In summary, through the mutual influence of the above-mentioned multiple effects, firstly, by ensuring that the specified amount of culture nutrient raw materials is provided at multiple layers of positions from the outside to the inside of the culture medium, the nutrients required for the growth of King Oyster Mushroom can be provided without spraying the influencing liquid in the later stage. Then, by being able to provide King Oyster Mushroom with a variety of basic nutrients required for growth, and finally by covering the plastic mesh bag, and then placing the culture medium at the specified position, the culture medium is rotated to remove excess bacterial liquid, and the rotation speed of the culture medium needs to be maintained during rotation. In summary, the cost of growing King Oyster Mushroom can be reduced, and the growth rate of King Oyster Mushroom can be increased. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention. DETAILED DESCRIPTION

[0027] The following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0028] Example 1:

[0029] S1. Cultivation environment selection: Choose a place with good ventilation, suitable temperature and good humidity control for cultivation. The temperature range for the growth of King Oyster Mushroom is between 20℃. The temperature and humidity information of the King Oyster Mushroom growth environment is collected and sent to the central processing unit for processing. The central processing unit judges and compares the collected temperature and humidity information to determine whether the temperature and humidity in the King Oyster Mushroom growth environment are kept within the relative humidity of 80%. If they are not within the specified range, humidity and temperature control are required. The temperature and humidity need to be measured every 30 minutes using a temperature and humidity sensor;

[0030] S2. Preparation of culture medium: The culture medium uses 100 parts of corn stalks, 50 parts of rice straw, and 30 parts of sawdust as the main raw materials. The raw materials are cut into 5 cm lengths and then steamed for 30 minutes to eliminate bacteria and spores. 5 parts of nitrate nitrogen and 5 parts of ammonium nitrogen, 5 parts of calcium salt powder, 8 parts of sucrose, 5 parts of inositol, and 5 parts of organic additives are added. For the preparation of organic additives, 5 parts of casein hydrolyzate, 1-5 parts of yeast extract, and 5 parts of coconut juice are put into a mixer, and the speed is 10 r / min. After mixing for 5 minutes, the mixer is heated to 40°C, kept warm for 3 minutes, and then heated to 50°C and added. After 1 part of corn starch, mix at a speed of 10 r / min for 3 minutes, 5 parts of growth regulator, 5 parts of zeatin and 5 parts of 6-benzylpurine are added to the stirring tank, the speed of the stirring tank is controlled at 40 r / min, and the stirring time is 5 minutes. The stirring tank is heated to 50°C, kept warm for 3 minutes, cooled to room temperature, and then 2 parts of gibberellic acid are added and soaked for 5 minutes. After soaking, put on gloves to take out the growth regulator and place it in a holding bottle. Use a vacuum machine to remove the air in the holding bottle and then use it as a backup. Soak for 40 minutes. After the cooking is completed, the culture medium is cooled to an appropriate temperature.

[0031] S3, filling the interior of the culture medium: using a cutting tool to open a dot hole with a diameter of 10 cm at the center point of the culture medium, adding a water-permeable filter membrane inside the dot hole, then opening a plurality of middle holes with a diameter of 10 cm along the middle top position of the culture medium, placing a water-permeable filter membrane in the middle hole, and then opening edge holes with a diameter of 5 cm at the edge position of the culture medium, adding a water-permeable filter membrane to the edge hole, the number of dot holes is set to 1, the number of middle holes is set to 10, and the number of edge holes is set to 15, and the multiple edge holes are arranged in a circular equidistant distribution. The water-permeable filter membrane is mainly prepared by mixing 1 part of tert-butyl peroxyisopropylbenzene, 3 parts of fumaric acid, 5 parts of microcrystalline wax, and 2 parts of polyethylene wax, and then injection-molded by an injection molding machine. The water-permeable filter membrane needs to have a circular through hole with a cross-sectional shape of 5 mm;

[0032] S4, culture nutrient raw material addition: the culture nutrient raw material is placed in the extruder, the culture nutrient raw material consists of 15 parts of potatoes, 2 parts of glucose, 1 part of peptone, 0.5 parts of aluminum dihydrogen phosphate, 0.2 parts of magnesium sulfate, and 5 parts of agar powder, which are stirred and formed, and injected into the edge hole position by an injection machine, and the mobile injection machine injects the culture nutrient raw material into the middle hole position, and the mobile injection machine moves the culture nutrient raw material to the dot hole position for injection. The injection pressure of the injection machine is 1Mpa, and the injection time is 20S. The injection machine port needs to be wiped after each injection. The injection personnel require 3 people to supervise each other to see whether the culture nutrient raw materials in the dot hole position, the edge hole position, and the middle hole position are filled. After the injection is completed, a compactor is used for vertical compaction;

[0033] S5. Soaking and cooking: Place the compacted culture medium in a cooking chamber, heat it to 50°C and keep it warm for 5 minutes, then heat it to 80°C and keep it warm for 3 minutes, cool it to room temperature, and soak it in an appropriate amount of potassium iodate and sufficient deionized water for 5 minutes;

[0034] S6. Inoculation: Place the culture medium to be inoculated into a culture dish, then pour in a culture medium cooled to 45°C. Evenly spread the King Oyster Mushroom spawn on the culture medium at an inoculum rate of 200g per 100kg of culture medium. Shake gently and quickly to dilute the culture solution. Evenly cover the culture medium with the solution. Cover the culture medium with a plastic mesh bag and place the culture medium in a designated location. Drain excess culture solution by rotating the culture medium at a speed of 10 rpm. Drain the culture solution for 5 seconds. The person draining the culture solution should wear goggles.

[0035] S7. Culture medium stacking: stack the inoculated culture medium in a container with each layer no thicker than 15 cm. Use a bracket to support the outer wall of each culture medium, reinforce the culture medium and maintain the relative humidity at 80% and the temperature at 20°C.

[0036] S8. Culture condition control: Add water to the culture medium to maintain humidity and spray water regularly to moisten the mycelium. At the same time, maintain the temperature control range between 20°C and the relative humidity at 70%. Pay attention to ventilation during the growth period. Check the water level every day to avoid excessive evaporation and dryness. Spray water in time to maintain the relative humidity at 80%;

[0037] S9. Harvesting: When the King Oyster Mushrooms are full and the caps begin to open, they can be picked. Rotate the entire fruiting body and cut it off with scissors.

[0038] Example 2:

[0039] S1. Cultivation environment selection: Choose a place with good ventilation, suitable temperature and good humidity control for cultivation. The temperature range for the growth of King Oyster Mushroom is between 23°C. The temperature and humidity information of the King Oyster Mushroom growth environment is collected and sent to the central processing unit for processing. The central processing unit judges and compares the collected temperature and humidity information to determine whether the temperature and humidity in the King Oyster Mushroom growth environment are kept within the relative humidity of 85%. If they are not within the specified range, humidity and temperature control are required. The temperature and humidity need to be measured every 30 minutes using a temperature and humidity sensor;

[0040] S2. Preparation of culture medium: The culture medium uses 100 parts of corn stalks, 50 parts of rice straw, and 40 parts of sawdust as the main raw materials. The raw materials are cut into 8 cm lengths and then steamed for 50 minutes to eliminate bacteria and spores. 8 parts of nitrate nitrogen and 8 parts of ammonium nitrogen, 8 parts of calcium salt powder, 9 parts of sucrose, 8 parts of inositol, and 8 parts of organic additives are added. For the preparation of organic additives, 8 parts of casein hydrolyzate, 3 parts of yeast extract, and 15 parts of coconut juice are put into a mixer, and the mixer is heated to 40°C, kept warm for 4 minutes, and then heated to 60°C and corn stalks are added. After 2 parts of starch, the mixture was mixed at a speed of 15 r / min for 8 minutes, and 10 parts of growth regulators, 8 parts of zeatin and 8 parts of 6-benzylpurine were added to the stirring tank. The stirring tank speed was controlled at 50 r / min and the stirring time was 10 minutes. The stirring tank was heated to 70°C, kept warm for 4 minutes, cooled to room temperature, and then 4 parts of gibberellic acid were added and soaked for 8 minutes. After soaking, gloves were put on to take out the growth regulators and place them in a holding bottle. The air in the holding bottle was removed by a vacuum machine and then used as a backup. The mixture was soaked for 50 minutes. After the cooking was completed, the culture medium was cooled to an appropriate temperature.

[0041] S3, filling the interior of the culture medium: using a cutting tool to open a dot hole with a diameter of 15 cm at the center point of the culture medium, adding a water-permeable filter membrane inside the dot hole, and then opening a plurality of middle holes with a diameter of 15 cm along the middle top position of the culture medium, placing a water-permeable filter membrane in the middle hole, and then opening edge holes with a diameter of 8 cm at the edge position of the culture medium, adding a water-permeable filter membrane to the edge hole, the number of dot holes is set to 1, the number of middle holes is set to 10-15, the number of edge holes is set to 18, and the multiple edge holes are arranged in a circular equidistant distribution. The water-permeable filter membrane is mainly prepared by mixing 4 parts of tert-butyl peroxyisopropylbenzene, 8 parts of fumaric acid, 8 parts of microcrystalline wax, and 8 parts of polyethylene wax, and then injection-molded by an injection molding machine. The water-permeable filter membrane needs to have a circular through hole with a cross-sectional shape of 8 mm;

[0042] S4, culture nutrient raw material addition: the culture nutrient raw material is placed in an extruder, the culture nutrient raw material consists of 20 parts of potatoes, 3 parts of glucose, 4 parts of peptone, 0.8 part of aluminum dihydrogen phosphate, 0.8 part of magnesium sulfate, and 5-10 parts of agar powder, which are formed after stirring and injected into the edge hole position by an injection machine, and the mobile injection machine injects the culture nutrient raw material into the middle hole position. The mobile injection machine moves the culture nutrient raw material to the dot hole position for injection, and the injection pressure of the injection machine is 1.25Mpa, and the injection time is 25S. The injection machine port needs to be wiped after each injection, and the injection personnel need 4 people to supervise each other to see whether the culture nutrient raw materials in the dot hole position, the edge hole position, and the middle hole position are filled. After the injection is completed, a compactor is used for vertical compaction;

[0043] S5. Soaking and cooking: Place the compacted culture medium in a cooking chamber, heat it to 55°C and keep it warm for 8 minutes, then heat it to 90°C and keep it warm for 4 minutes. After cooling to room temperature, soak it in an appropriate amount of potassium iodate and sufficient deionized water for 8 minutes.

[0044] S6. Inoculation: Place the culture medium to be inoculated into a culture dish, then pour in a culture medium cooled to 45°C. Evenly spread the King Oyster Mushroom spawn on the culture medium at an inoculum rate of 250g per 100kg of culture medium. Shake gently and quickly to dilute the culture solution. Evenly cover the culture medium with the solution. Cover the culture medium with a plastic mesh bag and place the culture medium in a designated location. Drain excess culture solution by rotating the culture medium at a speed of 18 rpm. Drain the culture solution for 8 seconds. The person draining the culture solution should wear goggles.

[0045] S7. Culture medium stacking: Stack the inoculated culture medium in a container with each layer no thicker than 15 cm. Use a bracket to support the outer wall of each culture medium. Strengthen the culture medium and maintain the relative humidity at 85% and the temperature at 22°C.

[0046] S8. Culture condition control: Add water to the culture medium to maintain humidity and spray water regularly to moisten the mycelium. At the same time, maintain the temperature control range between 22°C and the relative humidity at 75%. Pay attention to ventilation during the growth period. Check the water level every day to avoid excessive evaporation and dryness. Spray water in time to maintain the relative humidity at 85%;

[0047] S9. Harvesting: When the King Oyster Mushrooms are full and the caps begin to open, they can be picked. Rotate the entire fruiting body and cut it off with scissors.

[0048] Example 3:

[0049] S1. Cultivation environment selection: Choose a place with good ventilation, suitable temperature and good humidity control for cultivation. The temperature range for the growth of King Oyster Mushroom is between 25℃. The temperature and humidity information of the King Oyster Mushroom growth environment is collected and sent to the central processing unit for processing. The central processing unit judges and compares the collected temperature and humidity information to determine whether the temperature and humidity in the King Oyster Mushroom growth environment are kept within the relative humidity of 90%. If they are not within the specified range, humidity and temperature control are required. The temperature and humidity need to be measured every 30 minutes using a temperature and humidity sensor;

[0050] S2. Preparation of culture medium: The culture medium uses 100 parts of corn stalks, 50 parts of rice straw, and 50 parts of sawdust as the main raw materials. The raw materials are cut into 10 cm lengths and then steamed for 60 minutes to eliminate bacteria and spores. 10 parts of nitrate nitrogen and 10 parts of ammonium nitrogen, 10 parts of calcium salt powder, 10 parts of sucrose, 10 parts of inositol, and 10 parts of organic additives are added. 10 parts of casein hydrolyzate, 5 parts of yeast extract, and 20 parts of coconut juice are put into a mixer, and the speed is 20 r / min. After mixing for 10 minutes, the mixer is heated to 40°C, kept warm for 5 minutes, and then heated to 80°C and added. After adding 3 parts of corn starch, the mixture was mixed at a speed of 25 r / min for 10 minutes. 15 parts of a growth regulator, which was prepared as zeatin, and 10 parts of 6-benzylpurine were added to the stirred tank. The stirring tank speed was controlled at 60 r / min and the stirring time was 15 minutes. The stirred tank was heated to 80°C, kept warm for 5 minutes, cooled to room temperature, and then 5 parts of gibberellic acid was added and soaked for 10 minutes. After soaking, gloves were put on to remove the growth regulator and place it in a holding bottle. The air in the holding bottle was removed by a vacuum machine and then used as a backup. The mixture was soaked for 60 minutes. After the cooking was completed, the culture medium was cooled to an appropriate temperature.

[0051] S3, filling the interior of the culture medium: using a cutting tool to open a dot hole with a diameter of 20 cm at the center point of the culture medium, adding a water-permeable filter membrane inside the dot hole, and then opening a plurality of middle holes with a diameter of 20 cm along the middle top position of the culture medium, placing a water-permeable filter membrane in the middle hole, and then opening edge holes with a diameter of 10 cm at the edge position of the culture medium, adding a water-permeable filter membrane to the edge hole, the number of dot holes is set to 1, the number of middle holes is set to 15, the number of edge holes is set to 20, and the multiple edge holes are arranged in a circular equidistant distribution. The water-permeable filter membrane is mainly prepared by mixing 5 parts of tert-butyl peroxyisopropylbenzene, 10 parts of fumaric acid, 10 parts of microcrystalline wax, and 10 parts of polyethylene wax, and then injection-molded by an injection molding machine. The water-permeable filter membrane needs to be opened with a circular through hole with a cross-sectional shape of 10 mm;

[0052] S4, culture nutrient raw material addition: the culture nutrient raw material is placed in the extruder, the culture nutrient raw material consists of 25 parts of potatoes, 5 parts of glucose, 5 parts of peptone, 1 part of aluminum dihydrogen phosphate, 1 part of magnesium sulfate, and 10 parts of agar powder, which are stirred and formed, and injected into the edge hole position by an injection machine, and the mobile injection machine injects the culture nutrient raw material into the middle hole position, and the mobile injection machine moves the culture nutrient raw material to the dot hole position for injection. The injection pressure of the injection machine is 1.5Mpa, and the injection time is 30S. The injection machine port needs to be wiped after each injection. The injection personnel require 5 people to supervise each other to see whether the culture nutrient raw materials in the dot hole position, the edge hole position, and the middle hole position are filled. After the injection is completed, a compactor is used for vertical compaction;

[0053] S5. Soaking and cooking: Place the compacted culture medium in a cooking chamber, heat it to 60°C and keep it warm for 10 minutes, then heat it to 100°C and keep it warm for 5 minutes. After cooling to room temperature, soak it in an appropriate amount of potassium iodate and sufficient deionized water for 10 minutes.

[0054] S6. Inoculation: Place the culture medium to be inoculated into a culture dish, then pour in a culture medium cooled to 45°C. Evenly spread the King Oyster Mushroom spawn on the culture medium at an inoculum rate of 300g per 100kg of culture medium. Shake gently and quickly to dilute the culture solution. Evenly cover the culture medium with the solution. Cover the culture medium with a plastic mesh bag and place the culture medium in a designated location. Drain excess culture solution by rotating the culture medium at a speed of 20r / min. Drain the culture solution for 10s. The person draining the culture solution should wear goggles.

[0055] S7. Culture medium stacking: Stack the inoculated culture medium in a container with each layer no thicker than 15 cm. Use a bracket to support the outer wall of each culture medium, reinforce the culture medium and maintain the relative humidity at 90% and the temperature at 25°C.

[0056] S8. Culture condition control: Add water to the culture medium to maintain humidity and spray water regularly to moisten the mycelium. At the same time, maintain the temperature control range between 25°C and the relative humidity at 80%. Pay attention to ventilation during the growth period. Check the water level every day to avoid excessive evaporation and dryness. Spray water in time to maintain the relative humidity at 90%;

[0057] S9. Harvesting: When the King Oyster Mushrooms are full and the caps begin to open, they can be picked. Rotate the entire fruiting body and cut it off with scissors.

[0058] The King Oyster Mushrooms prepared in Examples 1-3 were respectively tested for the number of days of cultivation, growth length, cost-saving rate, and whether the growth medium was missing growth substances. When they grew to the specified indicators, the data were recorded and the following data were obtained:

[0059] Planting days Growth length Growth width Cost savings Is the growth material in the culture medium missing? Example 1 44.5t 18.5cm 12.9cm 35% no Example 2 46.5t 16.5cm 11.8cm 25% no Example 3 45.6t 17.8cm 10.9cm 36.5% no

[0060] In summary, the data detection of planting days, growth length, cost saving rate, and whether the culture medium growth substance is missing in Example 1 is relatively excellent. Therefore, the present invention first ensures that the specified amount of culture nutrient raw materials is provided at multiple positions from the outside to the inside of the culture medium, and the nutrients required for the growth of King Oyster Mushroom can be provided without spraying the influencing liquid in the later stage. Then, a variety of basic nutrients required for the growth of King Oyster Mushroom can be provided. Finally, a plastic mesh bag is put on, and the culture medium is placed at a specified position. The excess bacterial liquid is removed by rotating the culture medium. The rotation speed of the culture medium needs to be maintained during rotation. This can not only save the cost of King Oyster Mushroom growth, but also increase the growth rate of King Oyster Mushroom.

[0061] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A pollution-free cultivation method for King Oyster Mushroom, characterized by: The specific steps are as follows: S1. Cultivation environment selection: Choose a place with good ventilation, suitable temperature and good humidity control for cultivation. The temperature range for the growth of King Oyster Mushroom is between 20-25℃ and the relative humidity is maintained at 80%-90%; S2. Culture medium preparation: The culture medium uses 100 parts of corn stalks, 50 parts of rice straw, and 30-50 parts of sawdust as the main raw materials. The raw materials are cut into 5-10 cm lengths and then steamed for 30 to 60 minutes to eliminate bacteria and spores. 5-10 parts of nitrate nitrogen and 5-10 parts of ammonium nitrogen, 5-10 parts of calcium salt powder, 8-10 parts of sucrose, 5-10 parts of inositol, 5-10 parts of organic additives, and 5-15 parts of growth regulators are added and soaked for 40-60 minutes. After steaming, the culture medium is cooled to an appropriate temperature. S3. Filling the culture medium: Using a cutting tool, a circular hole with a diameter of 10-20 cm is opened at the center of the culture medium. A water-permeable filter membrane is placed inside the circular hole. Multiple middle holes with a diameter of 10-20 cm are opened along the top of the culture medium. A water-permeable filter membrane is placed in the middle hole. Edge holes with a diameter of 5-10 cm are opened at the edge of the culture medium. A water-permeable filter membrane is placed in the edge holes. S4. Adding culture nutrient raw materials: The culture nutrient raw materials are placed into the extruder, injected into the edge holes by the injection machine, and then injected into the middle holes by the mobile injection machine. The mobile injection machine moves the culture nutrient raw materials to the dot holes for injection. After the injection is completed, the compactor is used to perform vertical compaction; S5. Soaking and cooking: Place the compacted culture medium in a cooking chamber, heat it to 50-60°C and keep it warm for 5-10 minutes, then heat it to 80-100°C and keep it warm for 3-5 minutes. After cooling to room temperature, soak it in an appropriate amount of potassium iodate and sufficient deionized water for 5-10 minutes. S6. Inoculation: Place the culture medium to be inoculated into a petri dish, then pour in a culture medium cooled to 45°C. Evenly spread the King Oyster Mushroom spawn on the culture medium at an inoculum rate of 200-300g per 100kg of culture medium. Quickly and gently shake the culture medium until the spawn is diluted and evenly covers the culture medium. Cover the culture medium with a plastic mesh bag and place the culture medium in a designated location. Excess spawn is removed by rotating the culture medium. S7. Culture medium stacking: Stack the inoculated culture medium in a container with each layer no thicker than 15 cm. Use a bracket to support the outer wall of each culture medium, reinforce the culture medium and maintain the relative humidity at 80%-90%, and the temperature control range is between 20-25°C. S8. Culture condition control: Add water to the culture medium to maintain humidity and spray water regularly to moisten the mycelium. At the same time, maintain the temperature control range between 20-25℃ and the relative humidity between 70% and 80%. Pay attention to ventilation during the growth period. Check the water level every day to avoid excessive evaporation and dryness. Spray water in time to maintain the relative humidity between 80% and 90%; S9. Harvesting: When the King Oyster Mushrooms are full and the caps begin to open, they can be picked. Rotate the entire fruiting body and cut it off with scissors.

2. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: In S1, the temperature and humidity information of the King Oyster Mushroom growth environment is collected and sent to the central processing unit for processing. The central processing unit judges and compares the collected temperature and humidity information to determine whether the temperature and humidity in the King Oyster Mushroom growth environment are within the set range. If not within the specified range, humidity and temperature control are required, and the temperature and humidity need to be measured once every 30 minutes using a temperature and humidity sensor.

3. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: The growth regulator in S2 is prepared as 5-10 parts of zeatin and 5-10 parts of 6-benzylpurine, which are added into a stirring kettle. The speed of the stirring kettle is controlled to 40-60 r / min, and the stirring time is 5-15 minutes. The stirring kettle is then heated to 50-80°C, kept warm for 3-5 minutes, and cooled to room temperature. 2-5 parts of gibberellic acid are then added and soaked for 5-10 minutes. After soaking, the growth regulator is taken out with gloves and placed in a holding bottle. The air in the holding bottle is removed by a vacuum machine and then used as a backup.

4. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: The organic additive in S2 is prepared by adding 5-10 parts of casein hydrolyzate, 1-5 parts of yeast extract, and 5-20 parts of coconut juice into a mixer, mixing at a speed of 10-20 r / min for 5-10 minutes, heating the mixer to 40°C, keeping the temperature for 3-5 minutes, and then heating it to 50-80°C, adding 1-3 parts of corn starch, and mixing at a speed of 10-25 r / min for 3-10 minutes.

5. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: The number of dot holes in S3 is set to 1, the number of middle holes is set to 10-15, the number of edge holes is set to 15-20, and the multiple edge holes are arranged in a circular ring with equal spacing.

6. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: The S3 water-permeable filter membrane is prepared by mixing 1-5 parts of tert-butyl peroxyisopropylbenzene, 3-10 parts of fumaric acid, 5-10 parts of microcrystalline wax, and 2-10 parts of polyethylene wax, and then injection-molded by an injection molding machine. The water-permeable filter membrane needs to have a circular through hole with a cross-sectional shape of 5-10 mm.

7. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: The culture nutrient raw materials in S4 are prepared by stirring 15-25 parts of potatoes, 2-5 parts of glucose, 1-5 parts of peptone, 0.5-1 part of aluminum dihydrogen phosphate, 0.2-1 part of magnesium sulfate, and 5-10 parts of agar powder.

8. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: The injection pressure of the injection machine in S4 is 1-1.5Mpa, and the injection time is 20-30S. The injection machine mouth needs to be wiped after each injection. 3-5 injection personnel are required to supervise each other to see whether the dot holes, edge holes, and middle holes are filled with culture nutrient raw materials.

9. The pollution-free cultivation method of King Oyster Mushroom according to claim 1, characterized in that: When the culture medium in S6 rotates, the rotation speed needs to be maintained at 10-20 r / min, and the bacterial liquid needs to be discharged for 5-10 seconds. The discharge personnel need to wear goggles.

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