Iron supplement tablet candy and method for preparing the same

Abstract

The application provides an iron supplement tabletting candy and a preparation method thereof, and the iron supplement tabletting candy comprises the following components in parts by weight: sorbitol 48-53 parts, hemoglobin peptide 35-40 parts, needle cherry fruit powder 4.1-4.8 parts, hemin chloride 1.2-1.5 parts, L-ascorbic acid 1.5-3.0 parts, and magnesium stearate 3.4-3.8 parts. The tabletting candy and the preparation method improve the viscosity of the mixture by using hemoglobin peptide in the preparation of the hemin tabletting candy, and the flowability and compressibility of the material can be improved by adding the flow agent powder in batches and premixing the material except part of the magnesium stearate and the hemoglobin peptide.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food, in particular, to a kind of iron supplement tablet candy and preparation method thereof. BACKGROUND

[0002] Iron plays an important role in the life process of organism; iron is involved in oxygen transport and storage, synthesis of cytochrome and metalloenzyme, cholesterin catabolism, collagen metabolism and neurotransmitter metabolism in the body, and is closely related to immune function of the body.

[0003] Iron deficiency or iron utilization disorder will lead to oxygen transport and storage capacity weakening, oxygen metabolism level reducing, cell viability decreasing; long-term iron deficiency will lead to iron deficiency anemia (IDA), accompanied by chronic fatigue, restlessness, immune function disorder, emotional or cognitive impairment, memory decline and other symptoms, and iron deficiency of infants and preschool children is more likely to affect brain development of children. WHO reported that in 2011, nearly half of the global 6-59 month old infants and nearly 1 / 3 of women of childbearing age were anemic.

[0004] At present, iron deficiency anemia is mainly treated by taking iron supplement, and a small part takes erythropoietin drugs; among them, iron supplement includes inorganic iron salt, small molecule organic acid iron salt, amino acid iron and hematin iron, among which hematin iron has the advantages of high bioavailability (absorption rate up to 80%), no in vivo iron accumulation poisoning and gastrointestinal irritation, and is currently widely used in food and health product industry.

[0005] Hemoglobin peptide is a small molecule peptide converted from globin in pig blood by modern biotechnology, has the characteristics of smaller molecular weight, high bioavailability, amino acid balance and resistance, and has the effects of nutritional fortification and enhancement of immune activity of the body. It is found that after protein is hydrolyzed by digestive enzymes, it is mainly absorbed in the form of small peptides, which is easier and faster to be absorbed and utilized by the body than completely free amino acids. Therefore, hemoglobin peptide used for iron supplement with hematin has attracted more and more attention.

[0006] And tablet candy is a common leisure food in our daily life, has the advantages of convenient carrying, easy storage and more health than general candy; but due to the poor mobility of raw materials, easy moisture absorption and no compressibility, tablet candy is prone to problems such as loose tablet, cracked tablet, poor hardness, sticky and so on. In recent years, various types of iron supplement have emerged in an endless stream, but there is no related report on tablet candy of hemoglobin peptide and hematin. In view of this, the present application is proposed. SUMMARY

[0007] The problem solved by the present application is to provide an iron supplement tablet candy.

[0008] To solve the above problems, the application provides an iron supplement tablet candy, which comprises the following components in parts by weight: sorbitol 48-53 parts, hemoglobin peptide 35-40 parts, needle cherry fruit powder 4.1-4.8 parts, hemin chloride 1.2-1.5 parts, L-ascorbic acid 1.5-3.0 parts, and magnesium stearate 3.4-3.8 parts.

[0009] Preferably, the iron supplement tablet candy comprises the following components in parts by weight: sorbitol 48-50 parts, hemoglobin peptide 38-40 parts, needle cherry fruit powder 4.2-4.5 parts, hemin chloride 1.3-1.5 parts, L-ascorbic acid 2.0-3.0 parts, and magnesium stearate 3.6-3.8 parts.

[0010] Preferably, the iron supplement tablet candy is composed of the following components in parts by weight: sorbitol 48 parts, hemoglobin peptide 40 parts, needle cherry fruit powder 4.2 parts, hemin chloride 1.5 parts, L-ascorbic acid 2.5 parts, and magnesium stearate 3.8 parts.

[0011] Preferably, the average particle size of the hemoglobin peptide is 123.67-469.53 μm. Preferably, the average particle size of the hemoglobin peptide is 198.76-326.94 μm, which has good compressibility and is beneficial to the preparation of the iron supplement tablet candy.

[0012] The application further provides a preparation method of the iron supplement tablet candy, which comprises the following steps:

[0013] Preparation of the first material: L-ascorbic acid is dissolved in an appropriate amount of 50% ethanol aqueous solution, needle cherry fruit powder is added, and the mixture is heated to 38 ℃ and stirred for 30 min; vacuum drying is performed, and the mixture is crushed to pass through a 65-mesh sieve for use;

[0014] Crushing and sieving: hemin chloride and sorbitol are sieved respectively for use;

[0015] Tabletting: hemin chloride, sorbitol, the first material, magnesium stearate, and hemoglobin peptide are added into a multidirectional motion mixer, and the mixture is mixed at a stirring speed of 10-18 r / min for 27-35 min;

[0016] Packaging: the tablet candy is packaged to obtain the iron supplement tablet candy.

[0017] Preferably, step S2 comprises: sieving the hemin chloride through a 60-mesh sieve and sieving the sorbitol through a 40-mesh sieve for use;

[0018] Preferably, the step S3 comprises adding the hemin, sorbitol, the first material and 1 / 2 magnesium stearate into the multi-directional motion mixer, mixing for 25-30 min at a stirring speed of 10-18 r / min, and then adding the magnesium stearate and hemoglobin peptide into the multi-directional motion mixer, mixing for 2-5 min at a stirring speed of 10-18 r / min.

[0019] Preferably, the stirring speed of the hemin, sorbitol, the first material and magnesium stearate during mixing is 14-16 r / min, and the stirring speed after adding the magnesium stearate and hemoglobin peptide is 10-14 r / min.

[0020] Compared with the prior art, the tabletted candy and the preparation method have the following technical effects: 1) the hemoglobin peptide is used in the preparation of the hemin tabletted candy to improve the viscosity of the mixture, and the flowability and compressibility of the material can be improved by adding the flow agent in batches and pre-mixing the material except for part of the magnesium stearate and hemoglobin peptide; 2) compared with the conventional stirring speed, the material has a good rest angle after mixing at a lower stirring speed, and the tabletting operation can be directly performed, thereby omitting the wet mixing and drying steps in the conventional preparation process, and the whole process is simpler and does not involve wet mixing and heating and drying during the preparation process; 3) the L-ascorbic acid is adsorbed by the acerola cherry powder in the aqueous ethanol solution during the preparation process, so that the L-ascorbic acid is combined with the acerola cherry powder through hydrogen bonds, which ensures the sweet and sour taste and is beneficial to the stability of hemin. DETAILED DESCRIPTION

[0021] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific embodiments of the present application are described in detail below. It should be noted that the technical features in the embodiments of the present application can be combined with each other.

[0022] Iron is an essential trace element for the human body, and exists in two forms in the body, i.e. circulating iron and storage iron, of which 60% to 70% exists in hemoglobin, which is an important component of hemoglobin, cytochrome enzymes, peroxidase, ribonucleotide reductase, etc., and participates in blood oxygen transport and oxidation-reduction metabolism. Iron deficiency or iron utilization disorder will lead to iron deficiency anemia, and about 1.62 billion people in the world currently suffer from iron deficiency anemia, which is a health problem that needs to be urgently addressed. Most of the existing iron supplements are in the form of liquid drug preparations, which are inconvenient to carry and have poor user acceptance, and it is of practical significance to develop an iron supplement tabletted candy. Therefore, the applicant proposes the following technical scheme:

[0023] Embodiment 1

[0024] An iron supplement tablet candy comprises the following components by weight: sorbitol 51 parts, hemoglobin peptide 38 parts, cherry powder 4.8 parts, hematin chloride 1.2 parts, L-ascorbic acid 1.5 parts, and magnesium stearate 3.5 parts, and is prepared according to the following method:

[0025] 1) Preparation of the first material: dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add cherry powder, heat to 38°C and stir for 30 min; vacuum dry, and pulverize to pass through a 65-mesh sieve for use;

[0026] 2) Pulverization and sieving: pass hematin chloride through a 60-mesh sieve, and pass sorbitol through a 40-mesh sieve for use;

[0027] As an example of the present application, the hematin chloride and hemoglobin peptide are prepared according to the following method:

[0028] A1. Take 1500 ml of anticoagulated pig blood, centrifuge at 4500 rpm for 20 min, and collect 580 ml of the lower layer of blood cells; wash once with 900 ml of 0.9% NaCl, centrifuge at 3500 r / min for 30 min; add 800 ml of deionized water, freeze at -30°C for 150 min, and thaw at a temperature increase of 12°C / min to 32°C; after homogenization, freeze again at -28°C for 130 min, and thaw at a temperature increase of 10°C / min to 30°C;

[0029] A2. Add 400 ml of water, mix, and adjust the pH to 8.7; after adding 0.05% L-ascorbic acid, heat to 55°C, and add 0.8% of a composite enzyme for enzymatic hydrolysis for 8 h, wherein the composite enzyme is composed of alkaline protease and flavor protease at a ratio of 1:3;

[0030] A3. Adjust the pH of the enzymatic hydrolysis solution to 3.7, stand for 35 min, and then centrifuge at 4000 rpm for 15 min to collect the supernatant and the precipitate, respectively; desalt the supernatant by ion exchange resin, concentrate, freeze-dry, and pulverize to obtain hemoglobin peptide;

[0031] A4. Dissolve the precipitate in a sodium hydroxide solution at pH 10.0, filter out impurities using a ceramic membrane with a pore size of 0.5 μm at a membrane pressure of 1.8 MPa and a temperature of 22°C; load the magnetic resin into a resin column placed vertically and in an adjustable magnetic field, control the magnetic induction intensity to be 0.8 T, and pass the filtrate through the magnetic resin column (height:diameter ratio of 6:1, resin amount of 90 ml) at a flow rate of 1.8 BV / h; after adsorption, adjust the magnetic induction intensity to 1.4 T, and wash out impurities using 3 BV of 30% ethanol solution (pH 9.0) at a flow rate of 1.0 BV / h.

[0032] The sodium hydroxide solution is treated as follows: the agitator tank is filled with nitrogen in advance, the sodium hydroxide solution with pH 10.0 is added and heated to 50℃, vacuum is extracted to 0.015Mpa, and reduced pressure stirring is performed for 30min; the temperature is slowly decreased to 4℃ at a speed of 10℃ / h, nitrogen is introduced into the agitator tank to 0.15Mpa, and pressure stirring is performed for 30min; the temperature is slowly increased to 50℃ at a speed of 15℃ / h, and the pressure is slowly decreased to 0.015Mpa at a speed of 0.04Mpa / h, and stirring is performed for 30min; the temperature is slowly decreased to 4℃ at a speed of 10℃ / h, and nitrogen is slowly introduced into the agitator tank to 0.35Mpa at a speed of 0.04Mpa / h, and pressure stirring is performed for 30min, and it is placed at room temperature and normal pressure for standby.

[0033] The magnetic resin is prepared by the following method:

[0034] A401, 6.2g FeCl3.6H2O and 2.2g FeCl2.4H2O are weighed and dissolved in 180ml deionized water, N2 is introduced for protection, stirring is performed under 80℃ water bath, NH3.H2O is added, pH is adjusted to 10.0, after reaction for 25min, deionized water is washed, and vacuum drying is performed at 55℃ to obtain magnetic particles; methanol, magnetic particles and tetraethyl orthosilicate are mixed according to 5:1:0.2, reaction is performed at 45℃ for 3.5h to obtain modified magnetic particles;

[0035] A402, acrylonitrile 10 parts, styrene 15 parts, divinylbenzene 70 parts, triallyl isocyanurate 20 parts, methyl acrylate 12 parts, gasoline 36 parts, diphenyl peroxide 1.2 parts, and modified magnetic particles 5 parts are uniformly mixed as an oil phase; deionized water 600 parts, calcium oxide 55 parts, sodium chloride 24 parts, and polyvinyl alcohol 0.9 parts are uniformly mixed as an aqueous phase;

[0036] A403, the oil phase is added to the aqueous phase and stirred to disperse the oil phase into beads; the temperature is increased to 82℃ and the beads are shaped, then the temperature is continuously increased to 90℃, and reaction is performed for 2.5h; the reaction temperature is increased to 102℃, and reaction is performed for 1.5h;

[0037] A404, cooling to room temperature and filtering, repeating washing 2 times with 2 times (v / m) of anhydrous ethanol, and vacuum drying at 48℃ to obtain the magnetic macroporous resin.

[0038] A5, adjusting the magnetic induction intensity to 0.7T, eluting with 50% ethanol solution with pH 10.2 at a flow rate of 0.5BV / h and collecting the eluate; concentrating to 1 / 3 of the original volume at 48℃ under vacuum pressure-0.09MPa, filtering with 0.5μm filter membrane; stirring at a speed of 50rpm, adding 4mol / l HCl to the filtrate to adjust pH 5.2, cooling to-2℃ at a rate of 10℃ / h, keeping for 5h, and centrifuging at 3500rpm for 20min to collect the precipitate;

[0039] A6, keeping the precipitate to cool to-25℃ for 4h, vacuumizing to a vacuum degree of <100pa, slowly warming to-5℃, meanwhile adjusting the vacuum degree to 1.0kpa and keeping for 1h; meanwhile, using microwave under 2450MHZ for 1s every 10min until the water content in the sample is 15%; keeping the vacuum degree at 1.4kPa, and microwave drying under 2450MHZ for 4s every 5min until completely dried; the prepared hematin chloride has a purity of 99.3% and an extraction yield of 90.4% after detection.

[0040] As an example of the present application, the acerola cherry fruit powder is prepared as follows:

[0041] B1, selecting fruits with high maturity, full and bright, no rot and mildew, washing the acerola cherry fruits clean, adding 5% NaOH solution at 80℃, soaking for about 2min, taking out and placing in a container that can leak, controlling the water flow rate to wash the acerola cherry, then pouring into a glass container and stirring with a wooden stick, adding appropriate amount of sodium sulfite to make SO2 content reach 100mg / L, stirring the mixture, and removing acerola cherry seeds and peels that fall off through sieving.

[0042] B2, sending the acerola cherry mixture into a double-pass pulper to pulper (the first pass pulper has a screen hole diameter of 3mm, and the second pass pulper has a screen hole diameter of 0.6mm), to obtain acerola cherry pulp; adding 0.5% citric acid and 0.2% sodium chloride (i.e. de-astringent liquid) to the separated pulp, treating at 50℃ for 20min.

[0043] B3, after the treated pulp is treated by colloid mill, it is sent into a high-pressure homogenizer for second fine crushing; the obtained slurry is subjected to solid-liquid separation (the obtained filtrate is the first filtrate for standby), the filtered coarse residue (first filter residue) is added with enzyme preparation (pectinase 0.2% and cellulase 0.2%), heated to 45℃, and homogenized and kept for 2.5-5h, the obtained slurry is treated by colloid mill, and then sent into a homogenizer for fine treatment, to obtain second filtrate.

[0044] B4, mixing the first filtrate and the second filtrate, then entering a vacuum concentrator to concentrate, the temperature is 50℃, the vacuum degree is -0.08Mpa, and the concentration is to 1 / 3 of the original volume. The slurry after concentration is dosed with resistant starch and isomaltulose at 20% of the mass, the mass ratio of the added resistant starch and isomaltulose is 4:1, and the slurry is homogenized and dried at 55℃ and a vacuum degree of -0.08Mpa, and the iron supplement tablet candy is obtained.

[0045] 5) tabletting: hematin chloride, sorbitol, the first material and 1 / 2 magnesium stearate are added into a multi-directional motion mixer, mixed at a stirring speed of 10r / min for 30min, magnesium stearate and hemoglobin peptide are continuously added into the multi-directional motion mixer, and total mixing is carried out at a stirring speed of 10r / min for 5min;

[0046] 6) packaging: the tabletted candy is packaged, and the iron supplement tablet candy is obtained.

[0047] Example 2

[0048] An iron supplement tablet candy, comprising the following components in parts by weight: sorbitol 53 parts, hemoglobin peptide 35 parts, cherry fruit powder 4.1 parts, hematin chloride 1.5 parts, L-ascorbic acid 3.0 parts, and magnesium stearate 3.4 parts, is prepared according to the following method:

[0049] 1) preparation of the first material: L-ascorbic acid is dissolved in an appropriate amount of 60% ethanol aqueous solution, cherry fruit powder is added, heated to 35℃ and stirred for 30min; vacuum dried, and pulverized through a 65-mesh sieve for use;

[0050] 2) pulverization and sieving: hematin chloride is sieved through a 60-mesh sieve, and sorbitol is sieved through a 40-mesh sieve for use;

[0051] 3) tabletting: hematin chloride, sorbitol, the first material and 1 / 2 magnesium stearate are added into a multi-directional motion mixer, mixed at a stirring speed of 18r / min for 28min, magnesium stearate and hemoglobin peptide are continuously added into the multi-directional motion mixer, and total mixing is carried out at a stirring speed of 18r / min for 2min;

[0052] 4) packaging: the tabletted candy is packaged, and the iron supplement tablet candy is obtained.

[0053] Example 3

[0054] An iron supplement tablet candy, comprising the following components in parts by weight: sorbitol 48 parts, hemoglobin peptide 40 parts, cherry fruit powder 4.2 parts, hematin chloride 1.5 parts, L-ascorbic acid 2.5 parts, and magnesium stearate 3.8 parts, is prepared according to the following method:

[0055] 1) Preparation of the first material: dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add cherry powder, stir for 30 min; vacuum dry, crush to pass through a 65 mesh sieve for use;

[0056] 2) Crushing and sieving: crush hemin to pass through a 60 mesh sieve, crush sorbitol to pass through a 40 mesh sieve for use;

[0057] 3) Tabletting: add hemin, sorbitol, the first material and 1 / 2 magnesium stearate into a multi-directional motion mixer, mix for 25 min at a stirring speed of 15 r / min, continue to add magnesium stearate and hemoglobin peptide into the multi-directional motion mixer, mix for 3 min at a stirring speed of 12 r / min;

[0058] 4) Packaging: package the tabletted candy to obtain the iron supplement tabletted candy.

[0059] Comparative Example 1

[0060] An iron supplement tabletted candy, comprising the following components in parts by weight: sorbitol 51 parts, hemoglobin peptide 38 parts, cherry powder 4.8 parts, hemin 1.2 parts, L-ascorbic acid 1.5 parts, colloidal silicon dioxide 3.5 parts, and prepared according to the following method:

[0061] 1) Preparation of the first material: dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add cherry powder, heat to 38℃ and stir for 30 min; vacuum dry, crush to pass through a 65 mesh sieve for use;

[0062] 2) Crushing and sieving: crush hemin to pass through a 60 mesh sieve, crush sorbitol to pass through a 40 mesh sieve for use;

[0063] 3) Tabletting: add hemin, sorbitol, the first material and 1 / 2 magnesium stearate into a multi-directional motion mixer, mix for 30 min at a stirring speed of 10 r / min, continue to add magnesium stearate and hemoglobin peptide into the multi-directional motion mixer, mix for 5 min at a stirring speed of 10 r / min;

[0064] 4) Packaging: package the tabletted candy to obtain the iron supplement tabletted candy.

[0065] Comparative Example 2

[0066] An iron supplement tabletted candy, comprising the following components in parts by weight: sorbitol 53 parts, hemoglobin peptide 38 parts, cherry powder 4.8 parts, hemin 1.2 parts, L-ascorbic acid 1.5 parts, colloidal silicon dioxide 1.5 parts, and prepared according to the following method:

[0067] 1) Preparation of the first material: dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add cherry powder, heat to 38°C and stir for 30 min; vacuum dry, crush and pass through a 65 mesh sieve for use;

[0068] 2) Crushing and sieving: pass the hemin through a 60 mesh sieve, and pass the sorbitol through a 40 mesh sieve for use;

[0069] 3) Tabletting: add the hemin, sorbitol, first material and 1 / 2 magnesium stearate into a multi-directional motion mixer, mix at a stirring speed of 10 r / min for 30 min, continue to add magnesium stearate and hemoglobin peptide into the multi-directional motion mixer, and mix at a stirring speed of 10 r / min for a total of 5 min;

[0070] 4) Packaging: package the tabletted candy to obtain the iron supplement tabletted candy.

[0071] Comparative Example 3

[0072] An iron supplement tabletted candy, comprising the following components by weight: sorbitol 51 parts, hemoglobin peptide 38 parts, cherry powder 4.8 parts, hemin 1.2 parts, L-ascorbic acid 1.5 parts, and magnesium stearate 3.5 parts, is prepared according to the following method:

[0073] 1) Preparation of the first material: dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add cherry powder, heat to 38°C and stir for 30 min; vacuum dry, crush and pass through a 65 mesh sieve for use;

[0074] 2) Crushing and sieving: pass the hemin through a 60 mesh sieve, and pass the sorbitol through a 40 mesh sieve for use;

[0075] 3) Tabletting: add the hemin, sorbitol, first material and magnesium stearate into a multi-directional motion mixer, mix at a stirring speed of 10 r / min for 35 min;

[0076] 4) Packaging: package the tabletted candy to obtain the iron supplement tabletted candy.

[0077] Comparative Example 4

[0078] An iron supplement tabletted candy, comprising the following components by weight: sorbitol 51 parts, hemoglobin peptide 38 parts, cherry powder 4.8 parts, hemin 1.2 parts, L-ascorbic acid 1.5 parts, and magnesium stearate 3.5 parts, is prepared according to the following method:

[0079] 1) Preparation of the first material: dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add cherry powder, heat to 38°C and stir for 30 min; vacuum dry, crush and pass through a 65 mesh sieve for use;

[0080] 2) Pulverization and sieving: hematin chloride was sieved through a 60-mesh sieve, and sorbitol was sieved through a 40-mesh sieve for standby use;

[0081] 3) Tabletting: hematin chloride, sorbitol, the first material, and 1 / 2 magnesium stearate were added into a multi-directional motion mixer, mixed at a stirring speed of 50 r / min for 30 min, and then hemoglobin peptide and magnesium stearate were added into the multi-directional motion mixer, and total mixing was performed at a stirring speed of 150 r / min for 5 min;

[0082] 4) Packaging: the tabletted candy was packaged to obtain the iron supplement tabletted candy.

[0083] Comparative Example 5

[0084] An iron supplement tabletted candy, comprising the following components in parts by weight: sorbitol 51 parts, hemoglobin peptide 38 parts, cherry powder 4.8 parts, hematin chloride 1.2 parts, L-ascorbic acid 1.5 parts, and magnesium stearate 3.5 parts, was prepared according to the following method: hematin chloride was sieved through a 60-mesh sieve, and sorbitol was sieved through a 40-mesh sieve for standby use; hematin chloride, sorbitol, L-ascorbic acid, cherry powder, and magnesium stearate were added into a multi-directional motion mixer, mixed at a stirring speed of 10 r / min for 35 min; and the tabletted candy was packaged to obtain the iron supplement tabletted candy.

[0085] Verification Experimental Example

[0086] I. Influence of Hemoglobin Peptide Particle Size on Tabletting Performance

[0087] Hemoglobin peptide is a small molecule peptide converted from porcine globin by modern biotechnology, and has the effects of nutrition and immune enhancement. In addition, due to its high viscosity and anti-hygroscopicity, it can improve the viscosity and flowability of raw materials, and is beneficial to solve the problems of loose tablets, cracked tablets, and sticking. Hemoglobin peptide with different particle size distributions was prepared by grinding for 3 min, 5 min, 15 min, and 30 min, and iron-containing tabletted candy was prepared according to the method of Example 1, and the tabletting performance was determined, and the results are shown in Table 1.

[0088] The particle size and particle size distribution of hemoglobin peptide were determined by light scattering method. The specific determination method was carried out according to the general specification in the third method (light scattering method) of “0982 Particle Size and Particle Size Distribution Determination Method” in the fourth part of the People's Republic of China Pharmacopoeia 2015 edition (People's Republic of China Pharmacopoeia 2015 edition, edited by the State Pharmacopoeia Commission, China Medical Science and Technology Press, June 2015, page 132). Similarly, the determination methods of hardness, friability, and angle of repose are all prior art, and will not be described here.

[0089] Table 1 Influence of different particle sizes on tabletting performance

[0090]

[0091] As shown in the above table, the particle size distribution of hemoglobin peptide can affect the compressibility of tabletted candy; when the particle size is too small or too large, the hardness is low, resulting in poor compressibility, therefore, the particle size of hemoglobin peptide is 198.76 to 326.94 μm, which is beneficial to the preparation of tabletted candy.

[0092] II. Tabletting parameters of different samples

[0093] The tabletted candy samples prepared by examples 1-3 and comparative examples 1-4 were taken respectively, and the hardness, friability, angle of repose and appearance shape were detected, and the results are shown in table 2.

[0094] Table 2 Statistics of tabletting parameters of different samples

[0095]

[0096] The angle of repose is also commonly used as the collapse angle, which is a commonly used powder flowability model parameter in China, which is obtained by calculating the plane angle of the conical pile formed by the vertical flow of powder due to gravity, and generally it can be roughly considered that the angle less than 40° can meet the requirements of powder flowability in industrial production process.

[0097] As shown in table 2, the angle of repose of the powder before tabletting in examples 1-3 of the present application is less than 30°, which has good flowability; the appearance of the prepared tabletted candy is complete and smooth, the hardness is more than 5.5 kg / mm2 and the friability is less than 0.35%, and the tabletting performance is good; the hardness of the final tabletted candy of comparative example 1 is poor because hemoglobin peptide is not added; the powder flowability of comparative example 2 is not good because the amount of flow aid added is small, the angle of repose is 38.6°, and the hardness and friability of the tablet are not as good as examples 1-3; comparative example 3 uses the conventional mixing tabletting method, which has an influence on the powder flowability, tablet hardness and friability, and the final powder flowability and tabletted candy performance are not good; comparative example 4 uses the conventional rotation speed (50-200 rpm) to mix the materials, and the applicant found that the final angle of repose value is high, and the flowability of the final powder is not ideal.

[0098] The application is used for the preparation of tablet candy by hemoglobin peptide and hemin, which can effectively improve the viscosity of the material, and the flowability and compressibility of the material can be improved by adding the flow agent powder in batches and premixing the materials except part of magnesium stearate and hemoglobin peptide, so that the tabletting operation can be directly carried out after mixing, and the wet mixing and drying steps in the conventional preparation process are omitted, the whole process is simpler, and the preparation process does not involve wet mixing and heating drying conditions, and the stability of the sample is better. The accelerated three-month stability of the tablet candy prepared in examples 1-3 and comparative example 5 is investigated, and the results show that the retention rate of hemin in examples 1-3 is as high as 99.6% or more, while the retention rate of hemoglobin in comparative example 5 is 94.3%, which shows that the L-ascorbic acid is adsorbed in the ethanol aqueous solution by using the needle cherry fruit powder in the preparation process, so that the L-ascorbic acid is combined with the needle cherry fruit powder by hydrogen bond, which ensures the sweet and sour taste of the tablet candy and is beneficial to the stability of hemin.

[0099] Although the application is disclosed as above, the application is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the application, and the protection scope of the application should be limited by the scope defined in the claims.

Claims

1. An iron-supplemented compressed candy, characterized in that, It includes the following components in parts by weight: 48-53 parts sorbitol, 35-40 parts hemoglobin peptide, 4.1-4.8 parts acerola cherry fruit powder, 1.2-1.5 parts ferric heme chloride, 1.5-3.0 parts L-ascorbic acid, and 3.4-3.8 parts magnesium stearate; The ferric chloride heme and hemoglobin peptides were prepared using the following method: A1. After centrifuging anticoagulated pig blood, collect the lower layer of blood cells; wash once with 0.9% NaCl, centrifuge; add deionized water to homogenize, freeze at -30℃ for 150 min, and thaw at 32℃; after homogenization, freeze again at -28℃ for 130 min, and thaw at 30℃. A2. After mixing with water, adjust the pH to 8.7, add 0.05% L-ascorbic acid, heat to 55°C, add 0.8% compound enzyme for enzymatic hydrolysis for 8 hours. The compound enzyme is composed of alkaline protease and flavor protease in a 1:3 ratio. A3. Adjust the pH of the enzymatic hydrolysate to 3.7, let it stand, and then centrifuge to collect the supernatant and precipitate. The supernatant is desalted by ion exchange resin, then concentrated, freeze-dried, and pulverized to obtain hemoglobin peptides. A4. Dissolve the precipitate in a sodium hydroxide solution with a pH of 10.0, and filter it through a ceramic membrane with a pore size of 0.5 μm to remove impurities. Pack the magnetic resin into a vertically placed resin column and place it in an adjustable magnetic field, controlling the magnetic induction intensity to 0.8T. Filter the filtrate onto the magnetic resin column. After adsorption, adjust the magnetic induction intensity to 1.4T and wash with a 30% ethanol solution with a pH of 9.0 to remove impurities. A5. After adjusting the magnetic induction intensity to 0.7T, elute with 50% ethanol solution at pH 10.2 and collect the eluent; concentrate to 1 / 3 of the original volume, filter through a filter membrane; stir, add 4 mol / L HCl dropwise to the filtrate to adjust the pH to 5.2, and at the same time cool down to -2℃ at 10℃ / h, keep warm for 5h, and then centrifuge at 3500rpm for 20min to collect the precipitate. A6. After cooling the precipitate to -25℃, keep it at that temperature for 4 hours. Evacuate the vacuum to <100 Pa, slowly raise the temperature to -5℃, and simultaneously adjust the vacuum to 1.0 kPa and keep it at that temperature for 1 hour. At the same time, microwave the sample for 1 second every 10 minutes until the water content in the sample reaches 15%. Maintain the vacuum at 1.4 kPa and microwave dry for 4 seconds every 5 minutes until completely dry. The preparation method of the iron-supplemented compressed candy includes: 1) Preparation of the first material: Dissolve L-ascorbic acid in an appropriate amount of 50% ethanol aqueous solution, add acerola cherry fruit powder, heat to 38℃ and stir for 30 min; vacuum dry, pulverize and pass through a 65 mesh sieve for later use. 2) Crushing and sieving: Sift ferric chloride heme and sorbitol separately for later use; 3) Tableting: Add ferric chloride heme, sorbitol, the first material and 1 / 2 magnesium stearate to a multi-directional motion mixer and mix at a stirring speed of 10-18 r / min for 25-30 min. Then add magnesium stearate and hemoglobin peptide to the multi-directional motion mixer and mix at a stirring speed of 10-18 r / min for 2-5 min. 4) Packaging: The compressed candy is packaged to obtain iron-supplemented compressed candy.

2. The iron-supplementing compressed candy according to claim 1, characterized in that, It includes the following components in parts by weight: 48-50 parts sorbitol, 38-40 parts hemoglobin peptide, 4.2-4.5 parts acerola cherry fruit powder, 1.3-1.5 parts ferric heme chloride, 2.0-3.0 parts L-ascorbic acid, and 3.6-3.8 parts magnesium stearate.

3. The iron-supplementing compressed candy according to claim 2, characterized in that, It is composed of the following components in parts by weight: 48 parts sorbitol, 40 parts hemoglobin peptide, 4.2 parts acerola cherry fruit powder, 1.5 parts ferric heme chloride, 2.5 parts L-ascorbic acid, and 3.8 parts magnesium stearate.

4. The iron-supplementing compressed candy according to claim 1, characterized in that, The average particle size of the hemoglobin peptide is 123.67-469.53 μm.

5. The iron-supplementing compressed candy according to claim 1, characterized in that, Step S2 includes: passing ferric chloride heme through a 60-mesh sieve and sorbitol through a 40-mesh sieve, for later use.

6. The iron-supplementing compressed candy according to claim 1, characterized in that, The mixing speed of ferric chloride heme, sorbitol, the first material and 1 / 2 magnesium stearate is 14-16 r / min, and the mixing speed after adding magnesium stearate and hemoglobin peptide is 10-14 r / min.

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