A method and device for constructing an animal model of seawater drowning-induced lung injury

Abstract

The present invention belongs to the technical field of animal model construction, and specifically relates to a method and device for constructing an animal model of seawater drowning lung injury. The device includes a box body, a movable partition is provided in the box body, the partition is bendable, and the partition divides the interior of the box body into a water storage chamber and a drowning chamber; a stirring device is installed in the water storage chamber, and the stirring device includes a rotating shaft, a driving device and a stirring blade, the rotating shaft is installed on the inner wall of the water storage chamber, and a driving device for driving the rotating shaft to rotate is installed on the inner wall of the water storage chamber, and the stirring blade is installed on the rotating shaft. When the stirring blade rotates, it can contact the partition, and when in contact, the partition bends toward the drowning chamber. The present invention provides a method and device for constructing an animal model of seawater drowning lung injury, which can simulate the real-world drowning process, and the injury state of the constructed animal model is closer to the real state of seawater drowning injury.

Description

Technical Field

[0001] The present invention belongs to the technical field of animal model construction, and particularly relates to a method and device for constructing an animal model of seawater drowning-induced lung injury. Background Art

[0002] Traditionally, drowning is believed to consist of the following processes: first, entering the water, then feeling breathless, followed by swallowing water. After swallowing water, the stomach becomes irritated and vomited out, and then the water is aspirated into the lungs, causing suffocation and death. The most critical of these is the water in the stomach and lungs. Unlike ordinary freshwater drowning lung damage, freshwater has a low salt content, while seawater has a high salt content. For seawater drowning patients, because seawater is hypertonic (3.5% salt), aspiration of high-salinity water into the lungs causes pulmonary edema. In this case, the ventilation disorder is not in the airway, but due to alveolar edema. Therefore, the key to resuscitation is mouth-to-mouth and positive pressure ventilation.

[0003] Since the pathogenic mechanism of seawater drowning lung injury is different from that of ordinary freshwater drowning lung injury, and the clinical symptoms caused are also different, the establishment of an animal model of seawater drowning lung injury and the study of treatment options for seawater drowning lung injury have far-reaching clinical significance. The characteristics of university research work are that there are many students and teachers engaged in research, and a large number of animal model samples are required. For example, drug research or clinical treatment program research in universities first needs to go through multiple animal model tests with multiple samples. Therefore, for the study of seawater drowning lung injury, the frequency of seawater drowning lung injury animal models is required to be high. Therefore, easy-to-operate seawater drowning lung injury animal model construction equipment and supporting methods are very necessary.

[0004] The existing methods for constructing animal models of drowning-induced lung injury mainly include perfusion and drowning experiments using simulation devices. For example, the article "Cao Wanying, Jin Guangfa, Luo Ying, et al. Experimental observation of ulinastatin in the treatment of seawater drowning-induced acute lung injury in rats [J]. Chinese Journal of Respiratory and Critical Care Medicine, 2011(2):5.DOI:10.3969 / j.issn.1671-6205.2011.02.009" and "Hua Jing, Feng Huasong, Wang Qing, et al. Establishment of an acute lung injury model in rats induced by seawater drowning [J]. Progress in Modern Biomedicine, 2013(27):5.DOI:CNKI:SUN:SWCX.0.2013-27-012" use perfusion to construct injury models. This method is artificial perfusion. Although it can show the situation of lung injury to a certain extent, it is different from the real drowning reaction and cannot accurately reflect the results of the comprehensive systemic reaction, because the real drowning process includes the comprehensive effects of the animal's struggle. Another example is a drowning lung injury simulation device disclosed in CN215223912U, which includes a water inlet assembly, a water inlet device housing, a simulated seawater storage tank, a drug inlet, a water inlet, a stirring motor, stirring blades, a switch A, a water inlet pipe, a water pump, and a water supply pipe. Chemicals such as sodium chloride, calcium salts, and magnesium salts are added through the drug inlet, and fresh water is added to the simulated seawater storage tank through the water inlet. Switch A then controls the stirring motor to drive the stirring blades, stirring the mixture evenly to form a simulated seawater experimental liquid. This liquid then flows through the water inlet pipe to the water pump, which then delivers the simulated seawater to an observation box containing mice via a water supply pipe. While these devices can effectively simulate the formulation of seawater, the simulated seawater is added directly to the observation box containing mice via a water supply pipe, failing to simulate the motion of seawater in the form of real waves, and thus differs from drowning caused by real-world waves.

[0005] In summary, the problem existing in the prior art is that there is a lack of a simulation device for seawater drowning lung injury with a simulation effect closer to the real state. Summary of the Invention

[0006] In order to solve the above-mentioned technical problem of "lack of a simulation device for seawater drowning lung injury with a simulation effect closer to the real state", the present invention provides a method and device for constructing an animal model of seawater drowning lung injury, which can simulate the real-world drowning process. The injury state of the constructed animal model is closer to the real state of seawater drowning injury.

[0007] The present invention aims to provide a device for constructing an animal model of seawater drowning-induced lung injury, comprising a box body, wherein a movable partition is provided in the box body, the partition being bendable, and the partition body divides the interior of the box body into a water storage chamber and a drowning chamber;

[0008] A stirring device is installed in the water storage chamber, and the stirring device includes a rotating shaft, a driving device and a stirring blade. The rotating shaft is installed on the inner wall of the water storage chamber, and the inner wall of the water storage chamber is also installed with a driving device for driving the rotating shaft to rotate. The stirring blade is installed on the rotating shaft. When the stirring blade rotates, it can contact the partition, and when in contact, the partition bends toward the drowning chamber.

[0009] Preferably, in the above-mentioned device for constructing an animal model of seawater drowning-induced lung injury, the edge of the partition is wrapped with a waterproof sleeve, and the waterproof sleeve contacts the inner wall of the box.

[0010] Preferably, in the above-mentioned device for constructing an animal model of seawater drowning-induced lung injury, the stirring blade is an arc-shaped plate or a bucket, and when the stirring blade contacts the partition, the stirring blade bends toward the drowning chamber.

[0011] Preferably, in the above-mentioned device for constructing an animal model of seawater drowning lung injury, the rotating shaft is arranged parallel to the partition, and the rotating shaft is also arranged parallel to the bottom surface of the box. When the stirring blade is not in contact with the partition, the partition is arranged vertically or at an angle of 0-15° to the vertical direction. At this time, the distance between the rotating shaft and the partition is less than the distance between the rotating shaft and the side of the box body opposite to the partition.

[0012] Preferably, the above-mentioned device for constructing an animal model of lung injury induced by seawater drowning, the device for constructing an animal model of lung injury induced by seawater drowning further includes a switching device, the switching device including a telescopic component, a deformable component and a locking component, the telescopic component being mounted on a side of the partition facing the water storage chamber, and being capable of contracting under the abutment of the stirring blade, and being in an extended state when there is no abutment of the stirring blade, the telescopic component being in a deformable state, the deformable component being mounted on a side of the telescopic component facing the stirring blade, and the locking component being mounted on the inner wall of the water storage chamber and being arranged corresponding to the deformable component, and when the telescopic component is extended, the deformable component is in an expanded state, and the end of the deformable component is connected to the corresponding locking component.

[0013] Preferably, in the above-mentioned device for constructing an animal model of seawater drowning-induced lung injury, the telescopic component is a first spring;

[0014] Alternatively, the telescopic component includes a second spring and a docking plate, one end of the second spring is connected to the partition, and the other end is connected to one side of the docking plate, and the other side of the docking plate is connected to the deformation component.

[0015] Preferably, in the above-mentioned device for constructing an animal model of seawater drowning-induced lung injury, the deformable component is formed by two connecting rods rotatably connected. When the deformable component is unfolded, the two connecting rods are unfolded into a straight line, and the connecting rods are connected to the corresponding locking components. When the deformable component is deformed, the two connecting rods become V-shaped, and the connecting rods are separated from the corresponding locking components.

[0016] Preferably, the above-mentioned device for constructing an animal model of lung injury induced by seawater drowning further comprises a height adjustment device, which comprises a lifting component, a load-bearing component and a fixing component, wherein the lifting component is arranged at the bottom of the drowning chamber, the lifting component is connected to the load-bearing component, and the fixing component is detachably connected to the load-bearing component.

[0017] Preferably, the above-mentioned device for constructing an animal model of lung injury induced by seawater drowning further comprises a water circulation device, which comprises a water pipe and a circulation pump, one end of the water pipe being located at the bottom of the drowning chamber, and the other end passing through the partition and extending toward the water storage chamber, a circulation pump being provided in the water storage chamber, and the circulation pump being installed at the position of the water pipe located in the water storage chamber.

[0018] The present invention also provides a method for constructing an animal model of seawater drowning-induced lung injury, comprising:

[0019] Configure simulated seawater for backup;

[0020] The device for constructing a seawater drowning lung injury animal model is installed according to the device, and the device is placed in a ready state, wherein the partition separates the water storage chamber and the drowning chamber;

[0021] Place the animal in a drowning chamber;

[0022] Add simulated seawater into the water storage chamber, turn on the driving device, and when the stirring blade rotates, the partition is abutted and deformed, pouring and flowing the simulated seawater into the drowning chamber, simulating the drowning effect caused by real waves.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] Since in the real world, most cases of drowning occur at the seaside, where people or animals are knocked down by waves and subsequently drown, in the present invention, a stirring device is installed in the water storage chamber. The stirring device is used to stir the simulated seawater to produce a disturbance that simulates the effect of waves. The stirring blade is an arc-shaped plate or bucket-shaped, and when the stirring blade rotates to be located above the rotating shaft, it bends toward the drowning chamber. Then, when the stirring blade rotates, it carries a certain amount of simulated seawater like a tipping bucket, and can dump the simulated seawater into the drowning chamber during rotation, which is more similar to the effect of real waves.

[0025] Animals freely placed in the drowning chamber can move and struggle in the simulated seawater. However, if left to their own devices, their instinctive desire to survive and their conditioned reflexes to survive may lead to different states of activity. Some animals exhibit ideal drowning symptoms, while others struggle nervously and helplessly. If the simulated seawater level is too high, the animals may drown, making subsequent treatment experiments impossible. Some animals have strong self-rescue abilities and avoid drowning. To improve the success rate of establishing an animal model of seawater drowning-induced lung injury, the present invention also provides a height adjustment device.

[0026] In addition, since the waves in the real world are continuous, it is difficult for people or animals to stand up on their own after being knocked down by the waves. Most of the struggles are in the limbs and head. Therefore, the present invention provides a fixed component to control the lateral position of the animal, which also meets the needs of simulating real seawater drowning situations.

[0027] In order to simulate the situation where continuous waves hit and drown animals, the present invention also provides a water circulation device. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The three-dimensional structure of the device for constructing the seawater drowning lung injury animal model of the present invention Figure 1 .

[0029] Figure 2 for Figure 1 Longitudinal section of the box (excluding the box cover).

[0030] Figure 3 The three-dimensional structure of the device for constructing the seawater drowning lung injury animal model of the present invention Figure 2 .

[0031] Figure 4 for Figure 3 Longitudinal section of the box (excluding the box cover).

[0032] Figure 5 This is a working state diagram of the present invention.

[0033] Figure 6For the present invention Figure 1 Left view of the internal structure of the water storage chamber.

[0034] Figure 7 For the present invention Figure 1 Top view of the internal structure of the water storage chamber (ready state).

[0035] Figure 8 For the present invention Figure 1 Top view of the internal structure of the water storage chamber (working state)

[0036] Figure 9 This is a diagram of the fixed relationship between the load-bearing components and the animal. DETAILED DESCRIPTION

[0037] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below with reference to specific embodiments and drawings.

[0038] In the description of the present invention, unless otherwise specified, all reagents used are commercially available and all methods used are conventional techniques in the art.

[0039] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0040] The terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of the technical features indicated. Therefore, features specified as "first" or "second" may explicitly or implicitly include one or more of such features; and in the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0041] Example 1

[0042] A device for constructing an animal model of seawater drowning-induced lung injury, see Figure 1-2, including a box body 1, and the box body 1 is made of transparent material or opaque material. If a transparent material is used, it is convenient to observe the simulated seawater flow inside the box body 1 and the drowning situation of animals. If an opaque material is used, it can simulate the drowning situation of animals in the dark. The top of the box body 1 is in an open state, and the simulated seawater and the animals to be modeled are both fed into the box body 1 from the opening. A box cover 2 is provided on the top of the box body 1, and the function of the box cover 2 is to cover the open part of the box body 1, and it has the function of dustproof and waterproof. The box cover 2 and the box body 1 can be made of the same material or different materials.

[0043] A movable partition 3 is provided in the box body 1. The partition 3 is bendable and is made of a plate coated with a waterproof and rust-proof layer, such as an elastic steel plate, an acrylic plate or a memory alloy plate. These materials have a common feature, that is, they can bend under the action of an external force, and when the external force disappears, they can return to their original shape. In the present invention, before constructing the animal model, the partition 3 in the preparation state is set vertically or at an angle of 0-15° to the vertical direction. Preferably, the partition 3 in the preparation state is a flat plate. When the partition 3 is subjected to an external force, it is in a bent state. Preferably, the inner bottom surface of the box body 1 is set horizontally to facilitate the installation of the partition 3.

[0044] The partition 3 divides the interior of the box body 1 into a water storage chamber 11 on the left and a drowning chamber 12 on the right. The water storage chamber 11 contains simulated seawater, which is configured using a conventional formula, such as 24.72 grams of table salt, 0.67 grams of potassium chloride, 1.36 grams of calcium chloride, 4.66 grams of magnesium chloride, 6.29 grams of magnesium sulfate, 0.18 grams of sodium bicarbonate, and distilled water added to 1 liter. The drowning chamber 12 is used to place animals, such as mice and rats, for constructing an animal model of seawater drowning-induced lung injury. The edge of the partition 3 is wrapped with a waterproof sleeve 31, which is a rubber sleeve or a latex sleeve. The waterproof sleeve 31 contacts the inner wall of the box body 1, which can achieve a waterproof effect.

[0045] Since in the real world, most cases of drowning in seawater occur at the seaside, where people or animals are knocked down by waves and subsequently drown, in the present invention, a stirring device 4 is installed in the water storage chamber 11. The function of the stirring device 4 is to stir the simulated seawater and generate disturbances that simulate the effect of waves. The stirring device 4 includes a rotating shaft 41, a driving device, and a stirring blade 42. The rotating shaft 41 is installed on the inner wall of the water storage chamber 11. Note that it is not installed on the partition 3. The inner wall of the water storage chamber 11 is also installed with a driving device that drives the rotating shaft 41 to rotate. The driving device is used to control the rotation of the rotating shaft 41. The driving device is a unidirectional rotating motor or a bidirectional rotating motor. The driving device includes a fixing portion and a driving portion. The fixing portion is installed on the inner wall of the water storage chamber 11. The driving portion is connected to the rotating shaft 41. The switch of the driving device is set on the outer wall of the box body 1 to facilitate operation by the experimenter. The stirring blade 42 is installed on the rotating shaft 41.

[0046] Exemplarily, the stirring blade 42 is an arc-shaped plate or a bucket-shaped, and when the stirring blade 42 rotates to be located above the rotating shaft, the stirring blade 42 bends toward the drowning chamber 12. When the stirring blade 42 contacts the partition 3, the stirring blade 42 bends toward the drowning chamber 12. Then, when the stirring blade 42 rotates, it carries a certain amount of simulated seawater like a tipping bucket, and can pour simulated seawater into the drowning chamber 12 when rotating, which is closer to the effect of real waves hitting.

[0047] In order to cooperate with the operation of the stirring device 4 and more accurately simulate the effect of real waves, the rotating shaft 41 is set parallel to the partition 3. Preferably, the rotating shaft 41 is also set parallel to the bottom surface of the box body 1. Before constructing the animal model, the partition 3 in the preparation state is set vertically or at an angle of 0-15° to the vertical direction. Preferably, the partition 3 is tilted toward the drowning chamber 12, which facilitates the partition 3 to bend toward the drowning chamber 12. At this time, the distance between the rotating shaft 41 and the partition 3 is smaller than the distance between the rotating shaft 41 and the side of the box body 1 opposite to the partition 3. The water storage chamber 11 contains simulated seawater, and the first liquid level of the simulated seawater is high. See Figure 1 and Figure 2 When the stirring blade 42 rotates, it can contact the partition 3, but cannot contact the side of the box body 1 opposite to the partition 3, nor can it contact the bottom surface of the box body 1. When the stirring blade 42 contacts the partition 3, when the partition 3 is subjected to external force, it bends toward the drowning chamber 12, and the height of the bent partition 3 is lower than the height of the original first liquid level 111 of the water storage chamber 11. Then, when the partition 3 bends toward the drowning chamber 12, the simulated seawater enters the drowning chamber 12 ( Figure 4 The direction indicated by the black arrow is the direction of water flow), and the animals in the drowning chamber 12 are drowned. Figure 3-4 , plus the effect of the stirring blade 42 dumping the simulated seawater, this simulates the effect of real waves hitting the animals and drowning them. As the stirring blade 42 continues to rotate, the simulated seawater enters the drowning chamber 12, and the external force on the partition 3 is reduced or even disappears, and it returns to its original shape. Figure 5 The second liquid level 121 in the drowning chamber 12 submerges the animal, and the animal then exhibits symptoms such as struggling, agitation, and lung damage, which is closer to a real seawater drowning scene, with a high degree of restoration and great practical value.

[0048] It is important to note that the critical deformation force of the partition 3 must be carefully monitored. When the water in the water storage chamber 11 reaches the first liquid level 111, without the abutment of the stirring blades 42, the partition 3 does not bend toward the drowning chamber 12. In other words, the abutment of the stirring blades 42 is required to cause the partition 3 to deform. When the simulated seawater in the water storage chamber 11 enters the drowning chamber 12, the height of the original first liquid level 111 in the water storage chamber 11 drops. When the abutment of the stirring blades 42 disappears, the partition 3 automatically returns to its original shape.

[0049] It should be noted that the deformation of the partition 3 can also be controlled by a switch device, so that there is no need to detect the critical deformation force. Figure 6-8 The switch device includes a telescopic component 5, a deformable component 51 and a locking component 52. The telescopic component 5 is installed on the side of the partition 3 facing the water storage chamber 11, and can shrink under the abutment of the stirring blade 42. When there is no abutment of the stirring blade 42, the telescopic component 5 is in an extended state. The deformable component 51 is installed on the side of the telescopic component 5 facing the stirring blade 42, and the locking component 52 is installed on the inner wall of the water storage chamber 11, and is arranged corresponding to the deformable component 51. When the telescopic component 5 is extended, the deformable component 51 is in an expanded state, and the end of the deformable component 51 is connected to the corresponding locking component 52 and is limited. Then, the partition 3 cannot be deformed at this time, and the partition 3 and its external sleeve block the water storage chamber 11 from the drowning chamber 12, and the simulated seawater in the water storage chamber 11 cannot enter the drowning chamber 12. When the telescopic part 5 is abutted by the stirring blade 42 and contracts, the deformable part 51 is deformed and separated from the corresponding locking part 52, releasing the limiting effect. At this time, the partition 3 is deformed and bent toward the drowning chamber 12, and the simulated seawater in the water storage chamber 11 enters the drowning chamber 12.

[0050] Exemplarily, the telescopic component 5 is a first spring, one end of which is fixedly connected to the partition 3 and the other end is connected to the deformation component 51. Alternatively, the telescopic component 5 includes a second spring and a docking plate, one end of which is fixedly connected to the partition 3 and the other end is fixedly connected to one side of the docking plate, the other side of which is connected to the deformation component 51. The docking plate serves to increase the contact area with the stirring blade 42. Preferably, the docking plate is arranged parallel to the partition 3.

[0051] For example, the deformable component 51 is formed by two connecting rods 511 rotatably connected by a rotating component. When the deformable component 51 is unfolded, the two connecting rods 511 are extended into a straight line and connected to the corresponding locking components 52. When the deformable component 51 is deformed, the two connecting rods 511 become V-shaped and separate from the corresponding locking components 52. This structure is simple and easy to produce, and is easy to connect to the locking component 52.

[0052] For example, the rotating component is a hinge, hinge, or shaft. First, the rotating component is used to connect the two connecting rods 511 so that they can rotate relative to each other. Then, one of the rotating parts of the rotating component is connected to the telescopic component 5. For example, the hinge has a first rotating part and a second rotating part, the first rotating part is rotatably connected to the second rotating part, and a connecting rod 511 is connected to each of the first and second rotating parts. Then, the first rotating part or the second rotating part is connected to the docking plate of the telescopic component 5. Of course, it is also possible to connect one of the connecting rods 511 to the docking plate. This is sufficient as long as the working principle requirements of the present invention are met.

[0053] Exemplarily, the locking component 52 is a tube with an opening, fixedly attached to the inner wall of the housing 1. The opening of the tube faces its corresponding connecting rod 511, with each connecting rod 511 inserted into each tube. Because the first and second springs not only have contraction and extension functions but also a certain degree of twisting action, the ends of the connecting rods 511 can be manually and adaptively connected to the corresponding tubes. The tubes exert a restraining effect on the connecting rods 511, thereby exerting a restraining effect on the telescopic component 5 and the partition 3. Preferably, to enhance the restraining effect, the connecting rods 511 are snap-fitted to the tubes.

[0054] For example, in order to prevent the deformation component 51 from being excessively deformed and affecting the rotation of the stirring blade 42, a third spring is provided between the two connecting rods 511. The third spring is located between the stirring blade 42 and the rotating component, and the distance between the third spring and the rotating component is less than or equal to 3 cm.

[0055] In the above structure, the animals are freely within the drowning chamber 12 and can move and struggle in the simulated seawater. However, if the animals are allowed to struggle freely, their instinctive desire to survive and their conditioned reflexes to triumph may lead to different states of activity. Some animals will exhibit ideal drowning symptoms, while others will struggle nervously and helplessly. If the simulated seawater level is too high, the animals may drown, making subsequent treatment experiments impossible. Some animals have strong self-rescue abilities and float above the second liquid level 121, avoiding drowning. To improve the success rate of constructing an animal model of seawater drowning-induced lung injury, the present invention also provides a height adjustment device.

[0056] Reference Figure 5 and Figure 9 The height adjustment device includes a lifting component 6, a load-bearing component 61, and a fixed component 62. The lifting component 6 is located at the bottom of the drowning chamber 12 and has a height adjustment function. For example, the lifting component 6 includes an electric telescopic rod or a telescopic motor. The lifting component 6 has a fixed portion and a movable portion. The fixed portion is fixedly mounted on the bottom of the drowning chamber 12, and the movable portion is connected to the load-bearing component 61. The animal is placed on the load-bearing component 61 and is fixed to the load-bearing component 61 by the fixed component 62.

[0057] Exemplarily, the fixing component 62 is a mesh plate or a porous plate, and the fixing component 62 is a rope. The rope body is tied to the animal's waist, and then the end of the rope is tied to the fixing component 62. In this way, the animal's head, neck, limbs and other parts can move freely, but because the waist is tied to the fixing component 62, the height of the animal can be adjusted. Then the experimental operator can adjust the height of the animal by adjusting the height of the lifting component 6 to adapt to the drowning demand. Since the height of the animal is manually controlled, drowning or ineffective drowning caused by the animal's autonomous activities can be avoided, thereby improving the success rate of constructing an animal model of seawater drowning-induced lung injury. In addition, since the waves in the real world are continuous, it is difficult for people or animals to stand up on their own after being knocked down by the waves, and most of the struggling parts are the limbs and head. Therefore, even if the present invention is provided with a fixing component 62 to control the lateral position of the animal, it also meets the needs of simulating real seawater drowning.

[0058] In order to simulate the situation where continuous waves hit drowning animals, the present invention also provides a water circulation device, see Figure 5 The water circulation device includes a water pipe 7 and a circulation pump 71. One end of the water pipe 7 is located at the bottom of the drowning chamber 12, and the other end passes through the partition 3 and extends to the water storage chamber 11. Preferably, it passes through the bottom of the partition 3. A circulation pump 71 is provided in the water storage chamber 11, and the circulation pump 71 is installed at the position of the water pipe 7 located in the water storage chamber 11. When the circulation pump 71 is working, the water pipe 7 continuously pumps the simulated seawater in the drowning chamber 12 back to the water storage chamber 11, and the driving device continuously drives the rotating shaft 41 to rotate, and the stirring blade 42 continuously rotates, and the simulated seawater in the water storage chamber 11 is returned to the drowning chamber 12 in the form of simulated waves. Such a cycle can simulate the situation where continuous waves hit drowning animals.

[0059] Preferably, the load-bearing component 61 is rotatably connected to the lifting component 6 via a rotating shaft or a universal shaft, so that the angle of the load-bearing component 61 is adjustable, and the angle of the animal fixed thereon is also adjustable, which can simulate the drowning effect of water coming from different directions of the animal.

[0060] Illustratively, a mounting platform 72 is installed at the bottom of the water storage chamber 11, and the mounting platform 72 covers the bottom of the water storage chamber 11. The circulating pump 71 is fixed on the mounting platform 72. The water pipe 7 passes through and is fixed on the mounting platform 72, and the outlet of the water pipe 7 is located above the mounting platform 72. In this way, the lowest water level of the liquid in the water storage chamber 11 is higher than the lowest water level of the liquid in the drowning chamber 12, which helps the simulated seawater in the water storage chamber 11 to flow into the drowning chamber 12.

[0061] Example 2

[0062] A method for constructing an animal model of seawater drowning-induced lung injury, comprising:

[0063] Use existing technical formula to configure simulated seawater for standby use;

[0064] The apparatus for constructing an animal model of seawater drowning-induced lung injury was installed according to the apparatus of Example 1, and the apparatus was placed in a ready state. In the ready state, the partition 3 separated the water storage chamber 11 from the drowning chamber 12 .

[0065] The animal is placed in the drowning chamber 12 and, if a height adjustment device is provided, the animal's waist is secured to the securing member 62 .

[0066] Add simulated seawater into the water storage chamber 11, turn on the driving device, and when the stirring blade 42 rotates, it abuts and deforms the partition 3, pouring and flowing the simulated seawater into the drowning chamber 12, simulating the drowning effect caused by real waves.

[0067] Circulation pump 71 is turned on, causing water pipe 7 to continuously pump simulated seawater from drowning chamber 12 back into water storage chamber 11. The drive device continuously drives shaft 41 to rotate, causing stirring blades 42 to continuously rotate, thereby returning the simulated seawater from water storage chamber 11 to drowning chamber 12 in the form of simulated ocean waves. This cycle can simulate the situation of continuous ocean waves hitting drowning animals.

[0068] It should be noted that the component connection relationships not specifically mentioned in the present invention are all assumed to adopt the existing technology. Since they do not involve the invention point and are widely used in the existing technology, the structural connection relationships are not described in detail.

[0069] It should be noted that when numerical ranges are mentioned in the present invention, it should be understood that both endpoints of each numerical range and any value between the two endpoints may be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes preferred embodiments. Although preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they understand the basic inventive concepts. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0070] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A device for constructing an animal model of seawater drowning-induced lung injury, comprising a box (1), characterized in that: A movable partition (3) is provided in the box body (1), the partition (3) is bendable, and the partition (3) divides the interior of the box body (1) into a water storage chamber (11) and a drowning chamber (12); A stirring device (4) is installed in the water storage chamber (11), and the stirring device (4) includes a rotating shaft (41), a driving device and a stirring blade (42). The rotating shaft (41) is installed on the inner wall of the water storage chamber (11), and the inner wall of the water storage chamber (11) is also installed with a driving device for driving the rotating shaft (41) to rotate. The stirring blade (42) is installed on the rotating shaft (41). When the stirring blade (42) rotates, it can contact the partition (3), and when in contact, the partition (3) bends toward the drowning chamber (12); The device for constructing an animal model of lung injury caused by seawater drowning also includes a switch device, which includes a telescopic component (5), a deformable component (51) and a locking component (52). The telescopic component (5) is installed on the side of the partition (3) facing the water storage chamber (11), and can be retracted under the abutment of the stirring blade (42). When there is no abutment of the stirring blade (42), the telescopic component (5) is in an extended state. The deformable component (51) is installed on the side of the telescopic component (5) facing the stirring blade (42). The locking component (52) is installed on the inner side wall of the water storage chamber (11) and is arranged corresponding to the deformable component (51). When the telescopic component (5) is extended, the end of the deformable component (51) is connected to the corresponding locking component (52).

2. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 1, characterized in that: The edge of the partition (3) is wrapped with a water-proof sleeve (31), and the water-proof sleeve (31) contacts the inner wall of the box body (1).

3. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 2, characterized in that: The stirring blade (42) is an arc-shaped plate, and when the stirring blade (42) contacts the partition (3), the stirring blade (42) bends toward the drowning chamber (12).

4. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 3, characterized in that: The rotating shaft (41) is arranged parallel to the partition (3), and the rotating shaft (41) is also arranged parallel to the inner bottom surface of the box body (1). When the stirring blade (42) is not in contact with the partition (3), the angle between the partition (3) and the vertical direction is 0-15 degrees. At this time, the distance between the rotating shaft (41) and the partition (3) is smaller than the distance between the rotating shaft (41) and the side surface of the box body (1) opposite to the partition (3).

5. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 1, characterized in that: The telescopic component (5) is a first spring; Alternatively, the telescopic component (5) includes a second spring and a docking plate, one end of the second spring is connected to the partition (3), and the other end is connected to one side of the docking plate, and the other side of the docking plate is connected to the deformation component (51).

6. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 5, characterized in that: The deformable component (51) is formed by two connecting rods (511) being rotatably connected. When the deformable component (51) is unfolded, the two connecting rods (511) are unfolded into a straight line, and the connecting rods (511) are connected to the corresponding locking components (52). When the deformable component (51) is deformed, the two connecting rods (511) become V-shaped, and the connecting rods (511) are separated from the corresponding locking components (52).

7. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 1, characterized in that: The device for constructing a seawater drowning lung injury animal model further includes a height adjustment device, which includes a lifting component (6), a load-bearing component (61) and a fixing component (62). The lifting component (6) is arranged at the bottom of the drowning chamber (12), the lifting component (6) is connected to the load-bearing component (61), and the fixing component (62) is detachably connected to the load-bearing component (61).

8. The device for constructing an animal model of seawater drowning-induced lung injury according to claim 1, characterized in that: The device for constructing a seawater drowning lung injury animal model further includes a water circulation device, which includes a water pipe (7) and a circulation pump (71). One end of the water pipe (7) is located at the bottom of the drowning chamber (12), and the other end passes through the partition (3) and extends toward the water storage chamber (11). The water storage chamber (11) is provided with a circulation pump (71), and the circulation pump (71) is installed at the position of the water pipe (7) located in the water storage chamber (11).

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