Highly antioxidant peach gum polysaccharide and preparation method thereof
High-antioxidant peach gum polysaccharide was prepared by organic base nitrogen doping technology, which solved the problem of poor antioxidant properties of peach gum polysaccharide, enabling its wide application in cosmetics, functional foods and biomedical materials, simplifying the preparation process and reducing costs.
Patent Information
- Application Number
- CN202311380715.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-24
- Publication Date
- 2025-11-25
- Estimated Expiration
- 2043-10-24
AI Technical Summary
In existing technologies, peach gum polysaccharides have poor antioxidant properties, which limits their application in cosmetics, functional foods and biomedical materials. Furthermore, traditional modification methods are complex, costly, and cause serious environmental pollution, making it difficult to achieve large-scale preparation.
Nitrogen doping of peach gum polysaccharide was carried out using organic bases, and high antioxidant peach gum polysaccharide was prepared by hydrolysis and dialysis freeze drying. Nitrogen doping was achieved by reacting primary amines in the organic bases with peach gum polysaccharide, thereby improving its water solubility and antioxidant properties.
The preparation process is simple and low-cost, and the obtained peach gum polysaccharide has excellent water solubility and antioxidant properties, which broadens its application prospects in cosmetics, functional foods and biomedical materials.
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Figure CN117402266B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of natural polysaccharides, and particularly relates to a high-antioxidant peach gum polysaccharide and a preparation method thereof. BACKGROUND
[0002] Natural polysaccharides are a kind of natural polymers with good biocompatibility and rich in sources, and have been widely used in many fields. Antioxidant property is an important factor affecting the application of natural polysaccharides. Natural polysaccharides with good antioxidant property can play an important role in preventing and treating diseases. On the contrary, natural polysaccharides with poor antioxidant property are limited in application.
[0003] Peach gum polysaccharide is a natural polysaccharide extracted from natural crude peach gum. Although the yield of natural peach gum is very high, its utilization rate is low. This is because the peach gum polysaccharide prepared by the traditional hydrolysis method has poor antioxidant property to a certain extent, which limits its application. In order to overcome this problem, the commonly used method is to modify the prepared peach gum polysaccharide by chemical modification. However, the existing modification method has the disadvantages of complex operation process, long reaction period, certain pollution to the environment, high preparation cost and the like, and it is difficult to realize large-scale preparation and application. In order to avoid the high cost problem caused by the complicated preparation process and greatly improve the practicability of the preparation of peach gum polysaccharide, it is urgent to invent a simple and effective method for preparing peach gum polysaccharide with high antioxidant property in the field. SUMMARY
[0004] The water-soluble peach gum polysaccharide prepared by the traditional hydrolysis method from natural crude peach gum has poor antioxidant property, which seriously limits its application as a carrier material or functional additive in cosmetics, functional food, biomedical materials and the like. In order to solve the above technical problems, the application provides a high-antioxidant peach gum polysaccharide and a preparation method thereof. The method can realize nitrogen doping of the natural crude peach gum which is insoluble in water during hydrolysis, and obtain peach gum polysaccharide with excellent water solubility and antioxidant property. The nitrogen-doped high-antioxidant peach gum polysaccharide has a good application prospect in the fields of cosmetics, functional food and biomedical materials.
[0005] To achieve the above object, the application provides the following technical scheme:
[0006] The application provides a preparation method of high-antioxidant peach gum polysaccharide. The organic base is used for nitrogen doping of the peach gum polysaccharide, and the high-antioxidant peach gum polysaccharide is obtained.
[0007] Further, the organic base is an organic amine containing primary amine. The organic base selected in the application is an organic compound containing amino group in the molecule, and the selected organic base is an organic amine containing primary amine. The nitrogen atom on the primary amine has strong nucleophilicity, and is more easily reacted with the peach gum polysaccharide to realize nitrogen doping.
[0008] Further, the organic base includes one or more of ethylenediamine, diethylenetriamine and triethylenetetramine.
[0009] Further, the method includes the following steps:
[0010] The crude peach gum is crushed into crude peach gum powder, and then swelled by adding water and an organic base to obtain a crude peach gum suspension, and the crude peach gum suspension is hydrolyzed in an oil bath, dialyzed and freeze-dried to obtain the high-antioxidant peach gum polysaccharide.
[0011] Further, the particle size of the crude peach gum powder is 30-50 mesh.
[0012] Further, the volume ratio of the crude peach gum powder, water and organic base is 1:(15-20):(0.1-1).
[0013] Further, the swelling temperature is 20-35℃ (room temperature), and the swelling time is 4-8 hours.
[0014] Further, the specific steps of the swelling are as follows: the crude peach gum is crushed into peach gum powder by a crusher, and then deionized water and an organic base are added to swell the peach gum sufficiently at 20-35℃, and the peach gum suspension is stirred by a stirrer.
[0015] Further, the oil bath hydrolysis temperature is 90-110℃, the hydrolysis time is 2-8 hours, and the stirring speed is 1000-2000 rpm.
[0016] Further, the peach gum suspension is placed in an oil bath for stirring and heating hydrolysis, and then dialyzed in a dialysis bag after hydrolysis, and then placed in a refrigerator for freezing (-4 to -24℃), and then freeze-dried.
[0017] The application further provides a high-antioxidant peach gum polysaccharide prepared according to the above preparation method.
[0018] The application further provides the use of the high-antioxidant peach gum polysaccharide in the fields of cosmetics, functional food and preparation of biomedical materials.
[0019] Compared with the prior art, the application has the following advantages and technical effects:
[0020] (1) The application first uses nitrogen doping technology to improve the antioxidant property of natural polysaccharide. The conventional nitrogen doping technology is based on post-functional modification, which has the disadvantages of complicated operation process and high cost. Unlike the conventional nitrogen doping technology, the application uses an organic base to provide alkaline conditions for hydrolysis of natural crude peach gum, and at the same time, the carboxyl contained in peach gum polysaccharide can react with the amino contained in the organic base to realize the hydrolysis of natural peach gum and the nitrogen doping of peach gum polysaccharide. In addition, due to the doping of nitrogen element, the prepared nitrogen-doped peach gum polysaccharide has excellent water solubility and antioxidant property.
[0021] (2) The preparation method proposed in the application has the advantages of simple preparation process, short preparation period, low cost, and large-scale preparation, and solves the problem of poor antioxidant property of peach gum polysaccharide. The prepared high-antioxidant peach gum polysaccharide has good application prospect in the fields of cosmetics, functional food and biomedical materials, and is expected to promote the application of peach gum polysaccharide and the development and utilization of natural peach gum resources. BRIEF DESCRIPTION OF DRAWINGS
[0022] The accompanying drawings, which form a part of this application, are included to provide a further understanding of the application and are incorporated in and constitute a part of this application. The embodiments of the present application illustrated in the drawings and their descriptions are used to explain the present application and are not intended to limit the present application. In the drawings:
[0023] Figure 1 A photo of the nitrogen-doped high-antioxidant peach gum polysaccharide prepared in Example 1 of the application;
[0024] Figure 2 A photo of the nitrogen-doped high-antioxidant peach gum polysaccharide prepared in Example 1 of the application after being dissolved in water;
[0025] Figure 3 An X-ray photoelectron spectroscopy full spectrum of the nitrogen-doped high-antioxidant peach gum polysaccharide prepared in Example 1 of the application;
[0026] Figure 4 An N1s spectrum of the nitrogen-doped high-antioxidant peach gum polysaccharide prepared in Example 1 of the application;
[0027] Figure 5 The ABTS free radical scavenging rate determination results of peach gum polysaccharide of Example 1 (nitrogen-doped peach gum polysaccharide) and Comparative Example 1 (undoped peach gum polysaccharide). DETAILED DESCRIPTION
[0028] Now, various exemplary embodiments of the application will be described in detail, which should not be considered as limiting the application, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the application.
[0029] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. Additionally, for a range of values of, an intermediate value of the upper limit and the lower limit of the range is specifically disclosed. Each smaller range between any stated value or inferred value and any other stated or inferred value in the stated range is also specifically disclosed. The upper and lower limits of these smaller ranges can independently be included or excluded in the ranges.
[0030] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application. All documents mentioned herein are incorporated by reference to disclose and describe in full the methods and / or materials which are described herein. In case of conflict, the content of the present specification will control.
[0031] Many modifications and variations of the present application described in the specification are possible without departing from the scope or spirit of the application. Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. The specification and examples are illustrative only.
[0032] As used herein, the terms "comprise", "comprising", "include", "including", "have", "having" and the like are open-ended and do not exclude additional elements or steps.
[0033] The embodiment of the present application provides a preparation method of high-antioxidant peach gum polysaccharide, which uses an organic base to perform nitrogen doping on the peach gum polysaccharide, so as to obtain the high-antioxidant peach gum polysaccharide.
[0034] In the embodiment of the present application, the organic base is an organic amine containing a primary amine. The organic base selected in the present application is an organic compound containing an amino group in the molecule, and the selected one is an organic amine containing a primary amine. The nitrogen atom on the primary amine has strong nucleophilicity, and is more easily reacted with the peach gum polysaccharide to realize nitrogen doping. As a preferred, the organic base includes one or more of ethylenediamine, diethylenetriamine and triethylenetetramine.
[0035] In the embodiment of the present application, the preparation method of the high-antioxidant peach gum polysaccharide specifically includes the following steps:
[0036] The dried natural crude peach gum is broken into crude peach gum powder by a crusher, and then deionized water and an organic base are added to swell the crude peach gum at 20-35℃. After swelling, the crude peach gum is stirred into a peach gum suspension by a stirrer. The peach gum suspension is heated and hydrolyzed in an oil bath, and then is put into a dialysis bag for dialysis. After dialysis, the peach gum is freeze-dried to obtain the high-antioxidant peach gum polysaccharide.
[0037] In the preferred embodiment of the present application, the particle size of the crude peach gum powder is 30-50 mesh. The particle size of the crude peach gum powder directly affects the swelling time of the crude peach gum. If the particle size is too large, the swelling time will be doubled. The particle size of the prepared crude peach gum powder is limited by the crushing equipment used. At present, the laboratory crusher can only crush to the above mesh size. In theory, the smaller the particle size, the faster the swelling and the shorter the time required.
[0038] In the preferred embodiment of the present application, the volume ratio of the crude peach gum powder, water and organic base is 1:(15-20):(0.1-1).
[0039] In the preferred embodiment of the present application, the swelling temperature is 20-35℃ (room temperature), and the swelling time is 4-8 hours. The swelling temperature is room temperature, which does not require additional energy consumption and reduces the preparation cost.
[0040] In the preferred embodiment of the present application, the oil bath hydrolysis temperature is 90-110℃, preferably 95-110℃, the hydrolysis time is 2-8 hours, and the stirring speed is 1000-2000 rpm. The oil bath hydrolysis provides a stable working temperature and a high temperature range, which can quickly reach the temperature conditions required for polysaccharide hydrolysis, accelerate the breakage of polysaccharide molecules, and form smaller fragments or monosaccharides at high temperatures. At the same time, the carboxyl groups in the polysaccharide will undergo condensation reaction with the organic base to form amide bonds. The above parameters of the oil bath hydrolysis temperature are the best parameters tested by experiments. Under these conditions, the hydrolysis rate of peach gum polysaccharide is fast. If the temperature is too high, the peach gum polysaccharide will be carbonized. If the temperature is too low, the peach gum polysaccharide will not be hydrolyzed enough or the hydrolysis time will be too long. If the hydrolysis time is too short, the polysaccharide molecular weight will be too large, which is not conducive to water solubility and subsequent application. After a certain hydrolysis time, the molecular weight of the peach gum polysaccharide tends to be stable. If the hydrolysis time is prolonged, the peach gum polysaccharide will be severely degraded or even completely decomposed into small molecules, and the cost of hydrolysis will also increase.
[0041] In the embodiment of the present application, the freezing temperature of the refrigerator is -4 to -24℃.
[0042] The natural crude peach gum used in the embodiment of the present application is picked in the Yushan Park in Guilin, China. Ethylenediamine, diethylenetriamine and triethylenetetramine are purchased from Xilong Chemical Co., Ltd.
[0043] The temperature range of the room temperature in the embodiment of the present application is 20-35℃.
[0044] The technical solutions of the present application are further illustrated by the following examples.
[0045] Example 1
[0046] (1) 1 kg of dried natural crude peach gum was crushed into 50-mesh peach gum powder by a crusher, and then swelled at room temperature by adding 20 times the volume of deionized water and 0.1 times the volume of ethylenediamine. The swelling was carried out at room temperature for 6 hours, and after the swelling was completed, a peach gum suspension was obtained by stirring with a blender;
[0047] (2) The peach gum suspension obtained in step (1) was placed in an oil bath at 100℃ for hydrolysis, and the stirring speed was adjusted to 1500 rpm. The hydrolysis time was 6 hours, and a nitrogen-doped peach gum polysaccharide aqueous solution was obtained;
[0048] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) was dialyzed in deionized water for 48 hours, and after dialysis, it was placed in a refrigerator for 24 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powder).
[0049] The photo of the nitrogen-doped peach gum polysaccharide with high antioxidant activity prepared in Example 1 of the present application is shown in Figure 1 , and the photo of the nitrogen-doped peach gum polysaccharide with high antioxidant activity prepared in Example 1 of the present application after being dissolved in water is shown in Figure 2 , and Figure 1 , and Figure 2 It can be seen that it has excellent water solubility.
[0050] The X-ray photoelectron spectroscopy full spectrum of the nitrogen-doped peach gum polysaccharide with high antioxidant activity prepared in Example 1 of the present application is shown in Figure 3 , and the N1s spectrum is shown in Figure 4 , and Figure 3 , and Figure 4 It can be seen that nitrogen doping is successfully achieved during the hydrolysis process.
[0051] Example 2
[0052] (1) 1 kg of dried natural crude peach gum was crushed into 50-mesh peach gum powder by a crusher, and then swelled at room temperature by adding 15 times the volume of deionized water and 0.5 times the volume of organic base ethylenediamine. The swelling was carried out at room temperature for 4 hours, and after the swelling was completed, a peach gum suspension was obtained by stirring with a blender;
[0053] (2) The peach gum suspension obtained in step (1) was placed in an oil bath at 95℃ for hydrolysis, and the stirring speed was adjusted to 1500 rpm. The hydrolysis time was 6 hours, and a nitrogen-doped peach gum polysaccharide aqueous solution was obtained;
[0054] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) is dialyzed in deionized water for 24 hours, and after dialysis, it is frozen in a refrigerator for 12 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powdered).
[0055] Example 3
[0056] (1) 1 kg of dried natural coarse peach gum is crushed into peach gum powder of 50 mesh by a crusher, and then swelled at room temperature by adding 15 times the volume of deionized water and 1 times the volume of organic base ethylenediamine, the swelling is carried out at room temperature, and the swelling time is 8 hours, and after swelling, a peach gum suspension is obtained by stirring with a blender;
[0057] (2) The peach gum suspension obtained in step (1) is hydrolyzed in an oil bath at 100°C, the stirring speed is adjusted to 1500 rpm, and the hydrolysis time is 8 hours, to obtain a nitrogen-doped peach gum polysaccharide aqueous solution;
[0058] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) is dialyzed in deionized water for 24 hours, and after dialysis, it is frozen in a refrigerator for 12 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powdered).
[0059] Example 4
[0060] (1) 1 kg of dried natural coarse peach gum is crushed into peach gum powder of 30 mesh by a crusher, and then swelled at room temperature by adding 15 times the volume of deionized water and 0.5 times the volume of organic base triethylenetetramine, the swelling is carried out at room temperature, and the swelling time is 4 hours, and after swelling, a peach gum suspension is obtained by stirring with a blender;
[0061] (2) The peach gum suspension obtained in step (1) is hydrolyzed in an oil bath at 97°C, the stirring speed is adjusted to 1300 rpm, and the hydrolysis time is 6 hours, to obtain a nitrogen-doped peach gum polysaccharide aqueous solution;
[0062] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) is dialyzed in deionized water for 48 hours, and after dialysis, it is frozen in a refrigerator for 24 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powdered).
[0063] Example 5
[0064] (1) 1 kg of dried natural crude peach gum was crushed into 30 mesh peach gum powder by a crusher, then swelled in 20 times volume of deionized water and 0.5 times volume of organic base (a mixture of diethylene triamine and triethylene tetramine with a mass ratio of 1:1) at room temperature, the swelling was carried out at room temperature, the swelling time was 8 hours, after the swelling was completed, the peach gum suspension was obtained by stirring with a blender;
[0065] (2) The peach gum suspension obtained in step (1) was placed in an oil bath at 110°C for hydrolysis, the stirring speed was adjusted to 1000 rpm, the hydrolysis time was 2 hours, and a nitrogen-doped peach gum polysaccharide aqueous solution was obtained;
[0066] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) was dialyzed in deionized water for 48 hours, after dialysis, it was placed in a refrigerator for 24 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powder).
[0067] Example 6
[0068] (1) 1 kg of dried natural crude peach gum was crushed into 30 mesh peach gum powder by a crusher, then swelled in 20 times volume of deionized water and 0.5 times volume of organic base (a mixture of diethylene triamine and triethylene tetramine with a mass ratio of 1:1) at room temperature, the swelling was carried out at room temperature, the swelling time was 8 hours, after the swelling was completed, the peach gum suspension was obtained by stirring with a blender;
[0069] (2) The peach gum suspension obtained in step (1) was placed in an oil bath at 110°C for hydrolysis, the stirring speed was adjusted to 1000 rpm, the hydrolysis time was 2 hours, and a nitrogen-doped peach gum polysaccharide aqueous solution was obtained;
[0070] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) was dialyzed in deionized water for 48 hours, after dialysis, it was placed in a refrigerator for 24 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powder).
[0071] Example 7
[0072] (1) 1 kg of dried natural crude peach gum was crushed into 30 mesh peach gum powder by a crusher, then swelled in 20 times volume of deionized water and 0.5 times volume of organic base (a mixture of diethylene triamine and triethylene tetramine with a mass ratio of 1:1) at room temperature, the swelling was carried out at room temperature, the swelling time was 8 hours, after the swelling was completed, the peach gum suspension was obtained by stirring with a blender;
[0073] (2) The peach gum suspension obtained in step (1) was placed in an oil bath at 110°C for hydrolysis, the stirring speed was adjusted to 1000 rpm, the hydrolysis time was 2 hours, and a nitrogen-doped peach gum polysaccharide aqueous solution was obtained;
[0074] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) is dialyzed in deionized water for 48 hours, and after dialysis, it is frozen in a refrigerator for 24 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powdered).
[0075] Comparative Example 1
[0076] The same as Example 1, except that no organic base is used for nitrogen doping, and the specific preparation process is as follows:
[0077] (1) 1 kg of dried natural coarse peach gum is crushed into 50-mesh peach gum powder with a crusher, and then swelled in 20 times the volume of deionized water at room temperature for 6 hours. After swelling, a peach gum suspension is obtained by stirring with a stirrer;
[0078] (2) The peach gum suspension obtained in step (1) is hydrolyzed in an oil bath at 100°C, with a stirring speed of 1500 rpm, and a hydrolysis time of 6 hours, to obtain a peach gum polysaccharide aqueous solution;
[0079] (3) The nitrogen-doped peach gum polysaccharide aqueous solution obtained in step (2) is dialyzed in deionized water for 48 hours, and after dialysis, it is frozen in a refrigerator for 24 hours, and then freeze-dried to obtain nitrogen-doped peach gum polysaccharide with high antioxidant activity (powdered).
[0080] Comparative Example 2
[0081] The same as Example 1, except that the organic base is replaced with an inorganic base NaOH with a concentration of 0.1 mol / L for hydrolysis.
[0082] Comparative Example 3
[0083] The same as Example 1, except that 1 kg of dried natural coarse peach gum is crushed into 100-mesh peach gum powder with a crusher.
[0084] Comparative Example 4
[0085] The same as Example 1, except that 1 kg of dried natural coarse peach gum is crushed into 50-mesh peach gum powder with a crusher, and then swelled in 25 times the volume of deionized water and 0.5 times the volume of inorganic base NaOH with a concentration of 0.1 mol / L at room temperature.
[0086] Comparative Example 5
[0087] The same as Example 1, except that the peach gum suspension obtained in step (1) is hydrolyzed in an oil bath at 150°C.
[0088] Comparative Example 6
[0089] The same as Example 1, except that the hydrolysis time in step (2) is 12 hours.
[0090] Comparative Example 7
[0091] The same as Example 1, except that the swelling time in step (1) was 12 h.
[0092] Performance Test
[0093] The peach gum polysaccharide powder prepared from Example 1 (nitrogen-doped peach gum polysaccharide) and Comparative Example 1 (undoped peach gum polysaccharide) was subjected to 2,2-azino-di(3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt) (ABTS) radical scavenging rate determination according to the method of Yong Liu, Yi-Qun Du, Jun-Hui Wang, et al. Int. J. Biol. Macromol. 2014, 64, 63-68, and the results are shown in Table 1. Figure 5 Figure 5 It can be seen that the antioxidant property of the peach gum polysaccharide after nitrogen doping by the method of the present application is significantly enhanced.
[0094] The (ABTS) radical scavenging rate determination results of Examples 2-7 and Comparative Examples 2-7 are as follows:
[0095] The nitrogen-doped peach gum polysaccharide prepared by Example 2 has a scavenging effect of 25% at 0.5 mg / mL and reaches 90% at 2 mg / mL. The nitrogen-doped peach gum polysaccharide prepared by Example 3 has a scavenging effect of 50% at a concentration of 0.5 mg / mL and almost 100% at 1.5 mg / mL. The nitrogen-doped peach gum polysaccharide prepared by Example 4 has a scavenging effect of nearly 80% at a concentration of 1 mg / mL and reaches 100% at 1.5 mg / mL. The nitrogen-doped peach gum polysaccharide prepared by Example 5 has a scavenging effect of 20% at a concentration of 0.5 mg / mL and reaches 80% at 2 mg / mL. The nitrogen-doped peach gum polysaccharide prepared by Example 6 has a scavenging effect of 90% at 1.5 mg / mL. The nitrogen-doped peach gum polysaccharide prepared by Example 7 has a scavenging effect of 95% at 1.5 mg / mL.
[0096] The ABTS radical scavenging efficiency of the inorganic base hydrolyzed peach gum polysaccharide prepared in Comparative Example 2 is lower than that of the nitrogen-doped peach gum polysaccharide in Example 1. The nitrogen-doped peach gum polysaccharide in Example 1 almost reaches 100% scavenging efficiency at a concentration of 2 mg / mL, while the inorganic base hydrolyzed peach gum polysaccharide has only 15% scavenging efficiency at 2 mg / mL, which is because the amino group contained in the nitrogen-doped peach gum polysaccharide has reducing property and can also have the effect of scavenging free radicals.
[0097] In the comparative example 3, due to the larger particle size of the broken particles, the degree of hydrolysis is reduced, the water solubility of peach gum polysaccharide is worse, the contact area with ABTS free radicals is small, and the removal effect is worse, only 60% removal efficiency at a concentration of 2 mg / mL.
[0098] In the comparative example 4, more and higher concentration of NaOH was added in the swelling, but the ABTS free radical scavenging effect of peach gum polysaccharide was not better, and the removal efficiency was still only 15% at a concentration of 2 mg / mL.
[0099] In the comparative example 5, peach gum polysaccharide was carbonized at a hydrolysis temperature of 150°C, which was partially insoluble in water, and the removal effect on ABTS free radicals was reduced, and the removal efficiency was only 40% at a concentration of 2 mg / mL.
[0100] In the comparative example 6, the extension of the hydrolysis time did not improve its removal effect, and the solubility was also not improved, and only at a concentration of 2 mg / mL, the removal efficiency was close to 100%.
[0101] In the comparative example 7, the swelling time of the crude polysaccharide was prolonged, similar to the results of comparative example 6, the removal effect was not improved, the solubility was also not improved, and only at a concentration of 2 mg / mL, the removal efficiency was close to 100%.
[0102] The above is only the preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical scope disclosed in the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.
Claims
1. A method for preparing a high-antioxidant peach gum polysaccharide, characterized in that, Nitrogen doping of peach gum polysaccharide with an organic base yields the highly antioxidant peach gum polysaccharide; the organic base includes one or more of ethylenediamine, diethylenetriamine, and triethylenetetramine; the process includes the following steps: The crude peach gum was crushed into crude peach gum powder, then water and organic alkali were added to swell it to obtain a crude peach gum suspension. The crude peach gum suspension was hydrolyzed in an oil bath, dialyzed and freeze-dried to obtain the high antioxidant peach gum polysaccharide. The volume ratio of the crude gum powder, water, and organic alkali is 1:(15-20):(0.1-1); The temperature for oil bath hydrolysis is 90℃~110℃.
2. The method for preparing high antioxidant peach gum polysaccharide according to claim 1, characterized in that, The particle size of the coarse peach gum powder is 30 mesh to 50 mesh.
3. The method for preparing high antioxidant peach gum polysaccharide according to claim 1, characterized in that, The swelling is carried out at room temperature, and the swelling time is 4 to 8 hours.
4. The method for preparing high antioxidant peach gum polysaccharide according to claim 1, characterized in that, The hydrolysis time of the oil bath hydrolysis is 2 to 8 hours, and the stirring speed of the oil bath hydrolysis is 1000 rpm to 2000 rpm.
5. A highly antioxidant peach gum polysaccharide, characterized in that, It is prepared according to any one of claims 1 to 4.
6. The application of the high antioxidant peach gum polysaccharide according to claim 5 in the fields of cosmetics, functional foods and preparation of biomedical materials.
Citation Information
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Preparation method and application of peach gum polysaccharide and cosmetics
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Hydrolysis method of natural peach gum, peach gum alkaline hydrolysate, application of peach gum alkaline hydrolysate and facial cleanser
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