A whitening and anti-aging composition for skin care and application thereof, and an essence oil and a preparation method thereof

Abstract

The present application relates to the field of skin care products, and discloses a whitening and anti-aging composition for skin care products and application thereof, and an essence oil and a preparation method thereof. The whitening and anti-aging composition for skin care products contains an active substance combination and a compound essential oil and plant oil combination; the active substance combination contains glabridin, 4-butyl resorcinol, undecylenoyl phenylalanine, natural bisabalol, ascorbic acid tetraisopalmitate and teprenone; the compound essential oil and plant oil combination contains Damascus rose essential oil, magnolia essential oil, frankincense essential oil, Roman chamomile essential oil, carrot seed essential oil, passion fruit seed oil, red wolfberry seed oil, rose fruit oil and sea buckthorn fruit oil. The skin care product prepared from the whitening and anti-aging composition has a more moderate and efficient transdermal absorption rate, and has excellent anti-photoaging effect and whitening and freckle-removing effect.

Description

Technical Field

[0001] This invention relates to the field of skincare products, specifically to a whitening and anti-aging composition for use in skincare products and its application, and an essential oil and its preparation method. Background Technology

[0002] As we age, changes in our internal functions, as well as external environmental factors such as light damage, chemical and physical harm, can all promote and exacerbate the production and deposition of melanin, resulting in dull skin, age spots, sun spots, melasma, and other skin problems.

[0003] There are many methods and products on the market that claim to whiten skin, but based on the principle of similarity compatibility, lipid-based substances are more easily absorbed and penetrated by the skin.

[0004] In recent years, due to the skin's lipophilic nature, oil-based skincare products such as facial oils have become increasingly popular with consumers, and more and more facial oils are flooding the market. Currently, most whitening facial oils rarely combine modern biotechnology with traditional aromatherapy and phytotherapy, and their whitening mechanisms are unclear, their whitening pathways are singular, their efficacy is not significant, and their transdermal absorption rate is not ideal. Some whitening facial oils deliberately pursue a sweet fragrance and add a high proportion of artificial fragrances, resulting in a high allergy rate. Some whitening facial oils excessively pursue a pleasant skin feel and add a high proportion of volatile alkanes and various cyclic siloxanes, causing potential environmental damage. Some facial oils that do not contain alkanes and siloxanes but are pure plant oil systems often have a greasy and heavy feel on the skin.

[0005] The ideal whitening essence oil that perfectly balances high efficacy and gentle skincare, achieving outstanding whitening and skincare effects while also pursuing a soothing natural fragrance, and without adding environmentally harmful cyclic siloxanes, alkanes with no real skincare benefits, or various skin feel modifiers, represents the direction that future consumers will truly seek for high-quality whitening essence oils.

[0006] Therefore, there is an urgent need to develop a truly effective whitening and spot-removing essence oil that is gentle on the skin, non-irritating, and has good transdermal absorption. This would allow for a precise and scientific formula that can not only reduce and inhibit melanin at its source but also achieve highly effective transdermal absorption in an extremely gentle and skin-friendly manner, thus achieving remarkable anti-aging effects. Summary of the Invention

[0007] The purpose of this invention is to solve the problems of existing skin care products having poor whitening, spot removal, and wrinkle reduction effects, heavy texture, and poor absorption.

[0008] To achieve the above objectives, a first aspect of the present invention provides a whitening and anti-aging composition for use in skincare products, the whitening and anti-aging composition comprising an active ingredient combination and a compound essential oil and plant oil combination; the mass ratio of the active ingredient combination to the compound essential oil and plant oil combination is 1:0.5-15;

[0009] The active ingredient combination contains glycyrrhizin, 4-butylresorcinol, undecenoyl phenylalanine, natural bisabolol, ascorbate tetraisopalmitate and teprenone;

[0010] The compound essential oil and plant oil combination contains Damask rose essential oil, magnolia essential oil, frankincense essential oil, Roman chamomile essential oil, carrot seed essential oil, passion fruit seed oil, red goji berry seed oil, rosehip oil, and sea buckthorn fruit oil.

[0011] In the whitening and anti-aging composition, relative to 100 parts by weight of 4-butylresorcinol, the content of glycyrrhizin is 2-50 parts by weight, the content of undecylenoyl phenylalanine is 10-350 parts by weight, the content of natural bisabolol is 10-100 parts by weight, the content of ascorbic acid tetraisopalmitate is 50-1000 parts by weight, the content of teprenone is 5-200 parts by weight, and the content of Damask rose essential oil is 5-300 parts by weight. The content of the magnolia essential oil is 2-150 parts by weight, the content of the frankincense essential oil is 5-250 parts by weight, the content of the Roman chamomile essential oil is 2-150 parts by weight, the content of the carrot seed essential oil is 5-200 parts by weight, the content of the passion fruit seed oil is 50-500 parts by weight, the content of the red goji berry seed oil is 50-500 parts by weight, the content of the rosehip oil is 50-3000 parts by weight, and the content of the sea buckthorn fruit oil is 10-200 parts by weight.

[0012] A second aspect of the present invention provides the use of the whitening and anti-aging composition described in the first aspect in skin care products.

[0013] A third aspect of the present invention provides an essential oil containing excipients and the whitening and anti-aging composition described in the first aspect, wherein the total content of the whitening and anti-aging composition is not less than 50 wt%.

[0014] A fourth aspect of the present invention provides a method for preparing the essential oil described in the third aspect, the method comprising: (1) mixing the components in phase A to obtain a mixture I; wherein phase A contains caprylic / capric triglyceride, triglyceride (ethylhexanoate) and glycyrrhizin;

[0015] (2) Mix phase B with the mixture I for the second time to obtain mixture II; phase B contains Hallstar's Sensolene Light ET, ethyl macadamia oleate, dimethyl methoxybenzodihydropyranol, unsaponifiables of shea butter, 4-butylresorcinol and undecenoyl phenylalanine.

[0016] (3) Mix phase C with mixture II for the third time to obtain mixture III; phase C contains reduced coenzyme Q10 and natural bisabolol;

[0017] (4) The D phase is mixed with the mixture III for the fourth time to obtain the mixture IV; the D phase contains a compound essential oil and plant oil combination, jojoba seed oil, ascorbate tetraisopalmitate, comfrey oil, natural tocopherol, and teprenone.

[0018] The whitening and anti-aging composition for skincare products provided by this invention uses a combination of specific types of active ingredients and compound essential oils and plant oils as the main components. Skincare products prepared using such a whitening and anti-aging composition have a gentler and more efficient transdermal absorption rate, while also exhibiting excellent anti-photoaging effects. It can effectively resist oxidation and effectively inhibit the connection between tyrosinase and α-MSH, PGE2, ET1, ET3, endothelin, and interfere with the connection between keratinocytes and melanocytes, blocking the expression of PAR-2, thereby achieving the effect of whitening and removing blemishes.

[0019] The whitening and anti-aging composition provided by this invention can effectively quench UVA and UVB-induced reactive oxygen free radicals, prevent cell membrane lipid peroxidation, improve the survival rate of HaCat cells damaged by UVB light, promote the expression of anti-apoptotic factor B lymphoma / leukemia-2 mRNA, inhibit the generation of intracellular ROS, and reduce the cell apoptosis rate; at the same time, it protects keratinocytes from ultraviolet damage, achieves deep and lasting moisturization of the stratum corneum, effectively protects the activity of fibroblasts in the basal layer, and truly achieves anti-photoaging; it can also brighten skin tone and has a mood-healing effect.

[0020] The whitening and anti-aging composition provided by this invention does not achieve unhealthy whitening by physically covering or chemically exfoliating to promote the shedding of keratinocytes, nor does it cause toxicity to melanocytes or affect the normal physiological activity of the skin. This whitening and anti-aging composition can achieve highly efficient, green, gentle, and healthy whitening, and can prevent and inhibit pigmentation induced by skin inflammation.

[0021] The skincare products prepared from the whitening and anti-aging composition provided by this invention achieve a gentle, effective, and multi-dimensional whitening effect primarily through the following aspects:

[0022] First, it has antioxidant and photo-aging-resistant properties; second, it eliminates inflammation; third, it inhibits tyrosinase; and fourth, it antagonizes melanin production-promoting factors. In addition, the essential oil provided by this invention can provide the skin with multi-dimensional nutrients, stimulate the body's own repair ability, and improve cell vitality. While enhancing the skin barrier function, it can also increase the activity of superoxide dismutase and catalase in the body, activate the activity of telomerase, clear various free radicals in the body, and reduce the production of lipofuscin by acting on multiple targets.

[0023] Specifically, this invention is based on the following mechanism and through inventive research, thus completing this invention:

[0024] Appropriate sun exposure can activate 7-dehydrosterol in the skin, helping the body synthesize vitamin D. However, excessive sun exposure can induce photodamage and photoaging of the skin, as well as induce skin immunosuppression and oxidative stress, causing sunburn, pigmentation, and symptoms such as roughness, sagging, and telangiectasia.

[0025] UVA irradiation activates photosensitive substances in the body, producing reactive oxygen species (ROS), which indirectly cause oxidative damage to melanocytes, converting melanin precursors into melanin and accelerating the transport of melanosomes within melanocytes, thus causing immediate skin darkening. UVB irradiation, on the other hand, stimulates keratinocytes to secrete α-MSH, activates MCIR on melanocytes, promotes the synthesis of tyrosinase within melanocytes, and increases melanin synthesis. Simultaneously, UVB can also stimulate dendritic growth in melanocytes, prompting the rapid transport of melanosomes to keratinocytes, thereby leading to skin darkening.

[0026] People can use physical, chemical, and natural plant-based sunscreens to prevent photoaging and achieve whitening effects. However, traditional physical sunscreens, if not densely packed, can cause further oxidative stress and damage. They require very high-quality manufacturing processes, resulting in a thick, sticky texture that can feel uncomfortable and mask-like. They can also easily clog sebaceous and sweat glands, especially for sensitive, acne-prone skin. Traditional chemical sunscreens have unclear accumulation and metabolic pathways in the body, posing potential risks of skin irritation and endocrine disruption. While natural plant-based active ingredients may not offer the same sun protection as traditional physical and chemical sunscreens, they are gentle, safe, and have no side effects, effectively protecting the skin from photoaging.

[0027] The whitening and anti-aging composition provided by this invention combines Damask rose essential oil, Roman chamomile essential oil, passion fruit seed oil, goji berry oil and rosehip oil to achieve a gentle and effective anti-photoaging effect.

[0028] Among them, Damask rose essential oil is rich in citronellol, geraniol, and nerol, making it the most effective anti-photoaging essential oil among all known natural plant essential oils. It can protect keratinocyte genes, protect fibroblasts, and increase the synthesis of type III collagen. Roman chamomile essential oil is a collection of various mild, calming, and soothing ester compounds, rich in multiple short-chain esters. It is second only to Damask rose in anti-photoaging effects, also protecting keratinocytes from free radical damage and increasing the synthesis of type III collagen in the dermis. At the same time, chamomile essential oil can help reduce inflammation deep in the skin and has excellent moisturizing effects, protecting fibroblasts. Passion fruit seed oil contains lecithin and its derivatives, which can prevent the production of melanin in the skin. Passion fruit seed oil is also rich in polyphenols and flavonoids, which can effectively scavenge free radicals and prevent lipid oxidation.

[0029] The red goji berry seed oil in this composition is rich in polyunsaturated fatty acids, β-carotene, zeaxanthin, lutein, sitosterol, and tocopherol. These active ingredients can significantly improve the survival rate of HaCat cells damaged by UVB light, promote the expression of anti-apoptotic factor B lymphoma / leukemia-2 mRNA, inhibit intracellular ROS generation, and reduce cell apoptosis rate. Treatment with different concentrations of red goji berry seed oil with UVB-induced photodamage showed varying degrees of improvement in HaCat cell survival. Furthermore, red goji berry seed oil is rich in linoleic acid, which can also prevent lipid peroxidation damage; lutein, an active ingredient, can protect HaCaT cells from UV radiation by reducing pro-inflammatory mediators such as IL-6, TNF-α, and cyclooxygenase-2; phytosterols can also alleviate skin inflammation and protect it from UV damage; natural tocopherols can significantly reduce skin erythema, swelling, and skin allergies caused by UV radiation, and inhibit the occurrence of skin cancer caused by UV radiation. These active ingredients provide a material basis for effectively resisting UVB photodamage to HaCaT cells.

[0030] Meanwhile, red goji berry seed oil can significantly reduce UVB-induced apoptosis rate in HaCaT cells and related factors; the rosehip oil in this composition is rich in various active ingredients such as polyphenols, flavonoids, tocopherols, lutein, zeaxanthin, and β-carotene. Its total flavonoid content is 72.23 times that of soybean oil, its tocopherol content is 1.69 times that of soybean oil and 6.77 times that of olive oil; its zeaxanthin content is 11.52 times that of soybean oil and 5.5 times that of olive oil, and its β-carotene content is 1.52 times that of olive oil; it also contains up to 21 kinds of fatty acids, including 6 kinds of unsaturated fatty acids, which have different degrees of scavenging ability against four free radicals: DPPH, ABTS, hydroxyl radicals, and superoxide radicals.

[0031] The compound essential oil and plant oil combination in this invention contains a variety of monoterpenoids, esters, various flavonoids, polyphenols, polyunsaturated fatty acids, lutein, zeaxanthin, β-carotene, various natural tocopherols and phytosterols, which can effectively quench UVA and UVB-induced reactive oxygen free radicals, prevent cell membrane lipid peroxidation, protect keratinocytes from ultraviolet damage, achieve deep and long-lasting moisturization of the stratum corneum, effectively protect the activity of fibroblasts in the basal layer, and truly achieve anti-photoaging.

[0032] Besides ultraviolet radiation, inflammation is also a key factor in inducing dull skin and pigmentation. Incorrect skincare techniques, excessive hormones, acne, trauma, laser irradiation, or long-term chronic or acute skin inflammation can all activate the keratinocyte, forming a series of inflammatory mediators and cytokines. These substances promote melanocyte differentiation and melanin synthesis. The prostaglandin receptors PGE2 and PGF2 can stimulate melanocyte dendrite formation and activate tyrosinase, upregulating the level of PG receptors on melanocytes; the leukotriene compounds LTC4 and LTD4 also promote melanocyte proliferation. Furthermore, histamine released from mast cells binds to H1 and H2 receptors on melanocytes, inducing melanocyte dendritic growth and upregulating tyrosinase levels, resulting in the production of large amounts of melanin.

[0033] Therefore, a highly effective whitening formula needs to primarily eliminate inflammation and block the release of various inflammatory factors.

[0034] The whitening and anti-aging active composition provided by this invention contains glycyrrhizin, bisabolol, and Roman chamomile, all of which possess remarkable anti-inflammatory effects in different dimensions. Among them, glycyrrhizin is the most important component of glycyrrhiza flavonoids. It is a mild phytoestrogen with excellent antioxidant, neuroprotective, anti-inflammatory, and whitening and spot-fading properties. Glycyrrhizin has an aromatic ring with two phenolic hydroxyl groups and a tricyclic isoflavone core. It has an active olefin and carries two hydroxyl groups on its aromatic ring, which gives it strong antioxidant function and is crucial for inhibiting melanin synthesis. In addition, its isoprene flavonoid core can bind to estrogen receptors, inhibit cytochrome P450 3A4 enzyme (CYP3A4), and has anti-inflammatory activity. It can enhance the effects of steroids, significantly reduce peroxidase activity, and inhibit inflammatory mediators PGE2 (prostaglandin E2), NO, and various pro-inflammatory cytokines; therefore, it can inhibit tyrosinase involved in melanin production.

[0035] Glycyrrhizin induces non-competitive inhibition; while natural bisabolol, derived from natural sesquiterpenoid compounds, has both α and β configurations and possesses anti-inflammatory, antibacterial, antiviral, and ulcer-healing effects, making it widely used in the pharmaceutical industry. It is stable, has excellent skin compatibility, and inhibits melanin production. It can inhibit the production of peroxidase (a major substance causing inflammation in organisms), exhibiting good inhibitory effects on acute and chronic inflammation. It can inhibit the release of leukotrienes LTB4 and interleukin IL-1α, thereby reducing the stimulation of the skin by ultraviolet radiation and chemical factors; although it does not inhibit prostaglandin PGE2, it can inhibit CREB phosphorylation through the cAMP signaling pathway in melanocytes, reducing the expression of inflammatory factors and the production of tyrosinase, thus inhibiting α-MSH-induced melanin deposition. The specific amount of natural bisabolol in this invention has a significant effect on reducing pigmentation.

[0036] Roman chamomile is one of the most abundant essential oils in nature in terms of ester content. It not only possesses excellent anti-inflammatory properties but also deep-penetrating anti-inflammatory effects, reaching directly to the dermis. Its effects are gentle and long-lasting, and it also has high therapeutic value for emotions. High-quality Roman chamomile essential oil, rich in short-chain esters, has a mild and soothing sweet aroma. It can quickly calm the central nervous system and soothe emotional trauma, achieving inner harmony and tranquility. It is very suitable for individuals with sensitive minds and skin, and also for various types of inflamed skin. Skin and nerves are inherently interconnected, both differentiated from the ectoderm; they have always been closely related. People with a harmonious and peaceful mental state often have healthy, flawless skin, while those who are anxious, insecure, and irritable often have sensitive, oily skin and numerous skin problems. Therefore, advanced skin care should also consider the healing and conditioning of the mental state.

[0037] Tyrosinase is the most crucial rate-limiting enzyme and key enzyme in melanin production, and it is most closely related to sunspots, freckles, melasma, and skin tone. A superior whitening formula always revolves around the topic of highly effective tyrosinase inhibition. However, the most important premise remains achieving gentle and safe whitening without harming melanocytes. Therefore, the gentle yet highly effective combination of glycyrrhizin and 4-butylresorcinol is an excellent synergistic pairing. The specific mechanism by which glycyrrhizin inhibits tyrosinase has been detailed above and will not be repeated here. Glycyrrhizin's inhibition rate of tyrosinase is 15 times that of kojic acid and more than 200 times that of vitamin C, making it not only highly effective but also gentle. 4-Butylresorcinol is a very effective human tyrosinase inhibitor. Both in vitro and in vivo experiments have confirmed that 4-butylresorcinol is a very effective skin whitening agent with a strong inhibitory effect on melanin synthesis. Its inhibition of tyrosinase activity is antagonistic, and it can compete for the active site of tyrosinase as a substrate, exhibiting concentration dependence. It can directly inhibit tyrosinase without affecting the signaling pathways related to melanin synthesis. Furthermore, 4-butylresorcinol is a small molecule compound with a molecular weight of only 166.21 and is lipophilic, resulting in high transdermal absorption efficiency. When combined with glycyrrhizin, the effect is multiplied.

[0038] Besides tyrosinase, skin whitening and fading dark spots also require attention to antagonizing melanin-promoting factors produced by keratinocytes. Keratinocytes secrete cytokines that gradually regulate melanin synthesis, such as bFGF, α-MSH, SCF, ET, IL-1, IFN-γ, LT-B4, PGE-2, NGF, and GM-SCF. Among these, the most important, core, and crucial is α-MSH, melanocyte-stimulating hormone, which is produced and secreted by skin keratinocytes and induces the production of eumelanin. Among natural amino acids, phenylalanine is considered a key amino acid that interacts with the α-MSH receptor. Peptides containing phenylalanine and other derivative compounds can affect the binding of ligands to this receptor, activating or inhibiting α-MSH function.

[0039] The undecenoyl phenylalanine in the whitening and anti-aging composition provided by this invention possesses both lipophilic and hydrophilic groups, exhibiting better biocompatibility than phenylalanine. It has a strong affinity for the α-MSH receptor MC1-R, making it the best known α-MSH antagonist. It can competitively compete with α-MSH for cell receptors, significantly reducing melanin production in melanocytes and effectively reducing excessive facial pigmentation. Undecenoyl phenylalanine is mild and non-irritating; even at concentrations as high as 2%, it remains non-irritating. In this invention, undecenoyl phenylalanine is combined with compound essential oils and plant oils to create an anhydrous formula, ensuring it maintains its molecular structure and penetrates the dermis. If formulated as a water-soluble emulsion, it would have stringent pH requirements, easily become ionic, and easily precipitate, making it difficult to penetrate the living cell layer to exert its effects.

[0040] Furthermore, the whitening and anti-aging composition provided by this invention also contains ascorbate tetraisopalmitate (VCIP), teprenone, frankincense essential oil, pink pepper essential oil, carrot seed essential oil, and magnolia essential oil, which can provide the skin with multi-dimensional nutrients, stimulate the body's own repair ability, enhance the skin barrier function, act on multiple targets, and reduce the production of lipofuscin. Among them, ascorbate tetraisopalmitate is a lipophilic ascorbic acid derivative with excellent transdermal absorption function. It can inhibit intracellular tyrosinase activity and melanin production, reduce UV-induced cell and DNA damage, prevent lipid peroxidation and skin aging, and stabilize photothermal oxygen. Yasunobu Ochiai evaluated the effect of VCIP on UV-induced skin pigmentation. The results showed that after topical application of VCIP, the increase of cellular peroxides after UVB irradiation was significantly inhibited, and the tolerance of cells to reactive oxygen species (hydrogen peroxide, tert-butyl hydrogen peroxide) was enhanced, and the production of interleukin IL-1α and PGE2 in keratinocytes irradiated by UVB was reduced.

[0041] Teprenone, in particular, can maintain telomere length by aligning DNA-stimulating genes in keratinocytes. It is a gentle telomere-protecting ingredient that combats aging at the cellular level. It stimulates telomerase activity, preserving DNA sequences as completely as possible during cell division, extending cell lifespan, and enhancing cell activity, making it an excellent cell recharger. Simultaneously, teprenone can enhance skin barrier function, repair skin mucous membranes, increase hydration, and reduce the formation of sunspots. Teprenone can significantly alter skin structure, making it smoother, and has anti-aging properties, refining pores, evening skin tone, and addressing issues such as pigmentation, dullness, and sagging. Beyond anti-aging and repair, teprenone also has excellent whitening effects. This is because it can inhibit lipid peroxidation, preventing peroxides from cross-linking with normal cellular components to produce lipofuscin, a substance that is difficult to metabolize and easily deposited. It also reduces the breakdown of collagen and elastin, improving fine lines and resulting in a brighter, more translucent complexion.

[0042] Frankincense essential oil is a water-distilled extract from the gum-like resin secreted by the trunk of the frankincense tree (Boswellia spp.), a metabolic product secreted by the frankincense tree to heal its wounds. Frankincense is an ancient medicinal herb that has been refined over thousands of years, growing in harsh desert environments, and possesses strong stress-resistance and excellent antioxidant activity. Pink pepper essential oil contains δ-carene, α-pinene, β-pinene, and hydroquinone, which can help improve skin microcirculation, maintain skin flora balance, and effectively brighten skin tone.

[0043] Carrot seed oil is a volatile oil extracted from dried, mature carrot seeds using steam distillation. Rich in carotene, leucosterol, terpenes, sesquiterpenes, β-bisabolol, β-eugenol, α-asarone, baicalediene, bergamot acetate, and trans-α-bergamotene, it is an excellent natural antioxidant. Its aroma has a fresh and intense top note, followed by a sweet and mellow base with powdery and warm earthy notes. It can scavenge DPPH free radicals, ABTS free radicals, hydroxyl free radicals, and hydrogen peroxide. Carrot seed oil promotes liver cell regeneration and lowers blood cholesterol, possessing excellent detoxifying and revitalizing effects to help the body detoxify. It can also regenerate skin cells, soothe and reduce inflammation, and promote blood and lymphatic circulation. The most significant characteristic of carrot seed oil is its ability to effectively lighten blemishes. Long-term use can treat dull skin, liver spots, and age spots, revitalizing the skin and helping to improve its immunity.

[0044] Among them, magnolia essential oil is the essential oil of the genus Michelia in the family Magnoliaceae. Magnolia essential oil is rich in various terpenes, alcohols, esters, ketones and triterpenoids. Among them, linalool and linalool derivatives have the highest content, reaching 46.96%. It also contains phenylethyl alcohol, vanillin, and up to 5.77% ketones, such as 3-hydroxy-2-butanone, cis-jasmone, dihydroβ-ionone and 2-indolone. The aromatic molecules of these ketones have the effect of promoting cell regeneration and have excellent whitening effects. In addition, magnolia essential oil also contains small amounts of citral, benzaldehyde, phenylacetaldehyde and vanillin. These components also have supplementary whitening effects and can inhibit tyrosinase, which are non-competitive inhibitors. Attached Figure Description

[0045] Figure 1 This is a comparison image of the improvement of spots in one of the subjects provided by the present invention after using the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0046] Figure 2 This is a comparison image of the improvement of spots in one of the subjects provided by the present invention after using the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0047] Figure 3 This is a comparison image of the improvement of spots in one of the subjects provided by the present invention after using the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0048] Figure 4 This is a comparison image of the improvement of brown spots in one of the subjects provided by the present invention after using the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0049] Figure 5 This is a comparison image of the improvement of brown spots in one of the subjects provided by the present invention after using the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0050] Figure 6 This is a comparison image of wrinkle improvement after one of the subjects provided by the present invention used the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0051] Figure 7 This is a comparison image of wrinkle improvement after one of the subjects provided by the present invention used the essential oil prepared in Example 1 (left image is before use, right image is after use);

[0052] Figure 8 This is a comparison image of wrinkle improvement after one of the subjects provided by this invention used the essential oil prepared in Example 1 (left image is before use, right image is after use). Detailed Implementation

[0053] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0054] As previously stated, a first aspect of the present invention provides a whitening and anti-aging composition for use in skincare products, the whitening and anti-aging composition comprising an active ingredient combination and a compound essential oil and plant oil combination; the mass ratio of the active ingredient combination to the compound essential oil and plant oil combination is 1:0.5-15;

[0055] The active ingredient combination contains glycyrrhizin, 4-butylresorcinol, undecenoyl phenylalanine, natural bisabolol, ascorbate tetraisopalmitate and teprenone;

[0056] The compound essential oil and plant oil combination contains Damask rose essential oil, magnolia essential oil, frankincense essential oil, Roman chamomile essential oil, carrot seed essential oil, passion fruit seed oil, red goji berry seed oil, rosehip oil, and sea buckthorn fruit oil.

[0057] In the whitening and anti-aging composition, relative to 100 parts by weight of 4-butylresorcinol, the content of glycyrrhizin is 2-50 parts by weight, the content of undecylenoyl phenylalanine is 10-350 parts by weight, the content of natural bisabolol is 10-100 parts by weight, the content of ascorbic acid tetraisopalmitate is 50-1000 parts by weight, the content of teprenone is 5-200 parts by weight, and the content of Damask rose essential oil is 5-300 parts by weight. The content of the magnolia essential oil is 2-150 parts by weight, the content of the frankincense essential oil is 5-250 parts by weight, the content of the Roman chamomile essential oil is 2-150 parts by weight, the content of the carrot seed essential oil is 5-200 parts by weight, the content of the passion fruit seed oil is 50-500 parts by weight, the content of the red goji berry seed oil is 50-500 parts by weight, the content of the rosehip oil is 50-3000 parts by weight, and the content of the sea buckthorn fruit oil is 10-200 parts by weight.

[0058] Preferably, the mass ratio of the active ingredient combination to the compound essential oil and plant oil combination is 1:4-10. The inventors have discovered that the whitening and anti-aging composition obtained under this preferred embodiment has superior whitening and spot-removing effects and transdermal absorption.

[0059] Preferably, in the whitening and anti-aging composition, relative to 100 parts by weight of 4-butylresorcinol, the content of glycyrrhizin is 5-30 parts by weight, the content of undecylenoyl phenylalanine is 100-300 parts by weight, the content of natural bisabolol is 20-80 parts by weight, the content of ascorbic acid tetraisopalmitate is 100-800 parts by weight, the content of teprenone is 10-150 parts by weight, and the content of Damask rose essential oil is 20-250 parts by weight. The content of the magnolia essential oil is 5-120 parts by weight, the content of the frankincense essential oil is 10-200 parts by weight, the content of the Roman chamomile essential oil is 5-120 parts by weight, the content of the carrot seed essential oil is 10-150 parts by weight, the content of the passion fruit seed oil is 100-400 parts by weight, the content of the red goji berry seed oil is 100-400 parts by weight, the content of the rosehip oil is 150-2500 parts by weight, and the content of the sea buckthorn fruit oil is 20-150 parts by weight.

[0060] Preferably, relative to 100 parts by weight of 4-butylresorcinol, the compound essential oil and plant oil combination also contains 10-300 parts by weight of blood orange essential oil, 5-250 parts by weight of pink pepper essential oil, 50-1500 parts by weight of tomato seed oil, 100-1000 parts by weight of pomegranate seed oil, and 1000-4000 parts by weight of perilla seed oil.

[0061] More preferably, relative to 100 parts by weight of 4-butylresorcinol, the compound essential oil and plant oil combination also contains 20-200 parts by weight of blood orange essential oil, 2-100 parts by weight of pink pepper essential oil, 5-1000 parts by weight of tomato seed oil, 350-450 parts by weight of pomegranate seed oil, and 3000-3500 parts by weight of perilla seed oil. The inventors have found that the whitening and anti-aging composition obtained under this preferred embodiment is gentler on the skin and has superior whitening, spot-removing, wrinkle-reducing, and transdermal absorption effects.

[0062] Preferably, relative to 100 parts by weight of 4-butylresorcinol, the whitening and anti-aging composition also contains 100-200 parts by weight of an antioxidant.

[0063] More preferably, based on the total weight of the antioxidants, the antioxidants contain 1-10 wt% dimethylmethoxybenzodihydropyranol, 5-20 wt% reduced coenzyme Q10, and 50-85 wt% natural tocopherol. The inventors have found that the whitening and anti-aging composition obtained under this preferred embodiment has superior whitening and spot-removing effects.

[0064] As previously stated, a second aspect of the present invention provides the application of the whitening and anti-aging composition described in the first aspect in skin care products.

[0065] Preferably, the skincare product includes an essential oil.

[0066] As mentioned above, a third aspect of the present invention provides an essential oil containing excipients and the whitening and anti-aging composition described in the first aspect, wherein the total content of the whitening and anti-aging composition is not less than 50 wt%.

[0067] Preferably, the excipient is selected from at least one of the following: triglyceride (ethylhexanoate), Hallstar's Sensolene LightET, macadamia oleate, jojoba seed oil, comfrey oil, unsaponifiables of shea butter, and caprylic / capric triglyceride.

[0068] More preferably, based on the total weight of the excipients, the excipients contain 10-20 wt% triglyceride (ethylhexanoate), 1-5 wt% Hallstar's Sensolene Light ET, 5-15 wt% ethyl macadamia oleate, 40-65 wt% jojoba seed oil, 1-5 wt% comfrey oil, 1-5 wt% unsaponifiable shea butter, and 10-20 wt% caprylic / capric triglyceride. The inventors have found that the essential oil obtained under this preferred embodiment has a smoother, lighter feel on the skin, and exhibits superior whitening and spot-removing effects as well as better scalp absorption.

[0069] As previously described, a fourth aspect of the present invention provides a method for preparing the essential oil described in the third aspect, the method comprising:

[0070] (1) The components in phase A are first mixed to obtain mixture I; phase A contains caprylic / capric triglyceride, triglyceride (ethylhexanoate) and glycyrrhizin;

[0071] (2) Mix phase B with the mixture I for the second time to obtain mixture II; phase B contains Hallstar's Sensolene Light ET, ethyl macadamia oleate, dimethyl methoxybenzodihydropyranol, unsaponifiables of shea butter, 4-butylresorcinol and undecenoyl phenylalanine.

[0072] (3) Mix phase C with mixture II for the third time to obtain mixture III; phase C contains reduced coenzyme Q10 and natural bisabolol;

[0073] (4) The D phase is mixed with the mixture III for the fourth time to obtain the mixture IV; the D phase contains a compound essential oil and plant oil combination, jojoba seed oil, ascorbate tetraisopalmitate, comfrey oil, natural tocopherol, and teprenone.

[0074] In this invention, the preparation methods of phases A, B, C, D, E, and F can be conventional preparation methods in the art, which can be achieved by stirring.

[0075] Preferably, the conditions for the first mixing include: a temperature of 60-65°C, a stirring speed of 30-80 rpm, and a time of 10-30 min;

[0076] The conditions for the second mixing include: a temperature of 55-60℃, a stirring speed of 30-80 rpm, and a time of 20-40 min;

[0077] The conditions for the third mixing include: a temperature of 45-50℃, a stirring speed of 30-80 rpm, and a time of 10-40 min;

[0078] The conditions for the fourth mixing include: a temperature of 25-35℃, a stirring speed of 30-80 rpm, and a time of 10-40 min.

[0079] The present invention will now be described in detail through implementation.

[0080] In the following examples, unless otherwise specified, each part by weight represents 1g.

[0081] In the following examples, unless otherwise specified, all raw materials are commercially available products.

[0082] The raw material information used in the following examples is shown in Table 1.

[0083] Table 1

[0084]

[0085]

[0086] Example 1

[0087] This embodiment illustrates the preparation of the whitening and anti-aging composition of the present invention according to the formulation in Table 2 and the method described below for preparing the essential oil of the present invention.

[0088] Methods for preparing essential oils include:

[0089] (1) Mix the components in phase A to obtain mixture I; the conditions for the first mixing are: temperature 65℃, stirring speed 30rpm, and time 30min.

[0090] (2) Mix phase B with the mixture I to obtain mixture II; the conditions for the second mixing are: temperature 60℃, stirring speed 30rpm, and time 30min.

[0091] (3) The C phase is mixed with the mixture II for the third time to obtain the mixture III; the conditions for the third mixing are: temperature of 50°C, stirring speed of 30 rpm, and time of 10 min.

[0092] (4) The D phase is mixed with the mixture III for the fourth time to obtain the mixture IV; the conditions for the fourth mixing are: temperature of 25°C, stirring speed of 30 rpm, and time of 30 min.

[0093] Unless otherwise specified, the remaining examples follow a similar process to Example 1. The difference is that the formulas and parameters used in each example are different, as detailed in Table 2 (Note: Parameters not listed in Table 2 are the same as those in Example 1).

[0094] Table 2

[0095]

[0096] Table 2 (continued)

[0097]

[0098] Comparative Example 3

[0099] This comparative example follows a similar procedure to Example 1, except that the same mass of phenylethyl resorcinol is used in this comparative example to replace the 4-butylresorcinol in Example 1.

[0100] Everything else is the same as in Example 1.

[0101] The essential oil DS3 was prepared.

[0102] Comparative Example 4

[0103] This comparative example follows a similar procedure to Example 1, except that the same mass of rose geranium essential oil is used in this comparative example to replace the Damask rose essential oil in Example 1.

[0104] Everything else is the same as in Example 1.

[0105] The essential oil DS4 was prepared.

[0106] Comparative Example 5

[0107] This comparative example was conducted using a similar procedure to Example 1, except that the same mass of stearyl glycyrrhizate was used in this comparative example to replace glycyrrhizin in Example 1.

[0108] Everything else is the same as in Example 1.

[0109] The essential oil DS5 was prepared.

[0110] Comparative Example 6

[0111] This comparative example follows a similar procedure to Example 1, except that the same mass of dipalmitoyl hydroxyproline is used in this comparative example to replace undecenoyl phenylalanine in Example 1.

[0112] Everything else is the same as in Example 1.

[0113] The essential oil DS6 was prepared.

[0114] Comparative Example 7

[0115] This comparative example follows a similar procedure to Example 1, except that the same mass of acetylcurcumone is used in this comparative example to replace teprenone in Example 1.

[0116] Everything else is the same as in Example 1.

[0117] The essential oil DS7 was prepared.

[0118] Test case

[0119] I. Toxicological testing:

[0120] The essential oils prepared in the aforementioned examples were tested according to the multiple skin irritation test method in the "Cosmetic Safety Technical Specifications" (2015 edition).

[0121] Test subjects: Healthy adult New Zealand rabbits (female, weighing 2.0-2.5kg), 4 rabbits were tested in parallel.

[0122] Experimental Procedure: Before the experiment, the hair on both sides of the spine of the experimental animal was shaved, with each area measuring approximately 3cm x 3cm on the left and right sides. 0.5mL of essential oil was applied to the skin on the left side, covering an area of ​​2.5cm x 2.5cm; the right side was left untreated as a control. Application was performed once daily for 14 consecutive days. Starting from the second day, the hair was shaved before each application, and any residue on the skin surface was removed with water. Results were observed one hour later, and skin irritation reactions were scored according to the "Cosmetic Safety Technical Specifications" (2015 edition).

[0123] After the experiment, the total score over 14 days, the average score for each animal over 14 days, and the average score for each animal per day were calculated, and the intensity of skin irritation was graded.

[0124] Observation results: The average score of each animal over 14 days and the average score of each animal per day were 0, indicating that the essential oil provided by this invention has good safety.

[0125] II. Human Patch Test:

[0126] The essential oil prepared in Example 1 above was tested.

[0127] Subjects: Healthy women (i.e., non-allergic) aged between 20 and 53 years old, who met the criteria for voluntary inclusion, and 30 subjects were included in the trial.

[0128] Patch test method: Using the closed patch test method, 0.020 mL to 0.025 mL of essential oil is placed in the patch tester, and a hypoallergenic adhesive tape is applied to the back of the subject. After 24 hours, the essential oil is removed, and the skin reaction is observed at 0.5, 24, and 48 hours after removal. The results are recorded according to the skin reaction grading standards in the currently effective technical specifications (Cosmetic Safety Technical Specifications (2015 Edition)).

[0129] Experimental results: The human skin patch test results showed that all 30 subjects had negative skin reactions, indicating that the essential oil provided by this invention has good safety.

[0130] III. Efficacy Testing:

[0131] Experiments were conducted on the whitening essential oils prepared in the aforementioned examples and comparative examples, with 20 subjects participating in each type of essential oil.

[0132] Experimental method: After cleansing, apply 0.5mL of the essence oil to the face twice a day, morning and evening, for 8 consecutive weeks.

[0133] Using the Visia7 skin image analysis system from Canfield, USA, we detected and analyzed all facial aging and lesions (spots, brown spots, and wrinkles) from the epidermis to the dermis through photography.

[0134] (1) Spots: These refer to spots or other pigmentation on the skin surface that are visible to the naked eye, such as sunspots, freckles, pimples, acne marks, acne scars, moles, and vascular lesions. The higher the measured value, the more severe the spot condition.

[0135] (2) Brown spots: These reflect the condition of the superficial and deep layers of the skin, such as pigmentation, freckles, lentigines, and melasma. Brown spots appear when melanin is secreted without restraint (excessive secretion). The higher the measured value, the more severe the brown spots.

[0136] (3) Wrinkles: These are folds or creases in the skin, mainly crow's feet. The higher the measurement value, the more severe the wrinkles.

[0137] The results of the product use test after 8 weeks were compared with the initial values ​​before product use, and the improvement rates of spots, brown spots, and wrinkles were calculated. The results of the spot test are shown in Table 3, the results of the brown spot test are shown in Table 4, and the results of the wrinkle test are shown in Table 5.

[0138] The improvement rate was calculated as follows: {(mean of spot score (brown spot score / wrinkle score) of the first 20 subjects - mean of spot score (brown spot score / wrinkle score) of the same subjects after 8 weeks) / mean of spot score (brown spot score / wrinkle score) of the first 20 subjects} × 100%.

[0139] Table 3

[0140] Before use After use (8 weeks) Improvement rate (%) Example 1 29.659 16.302 45.04 Example 2 30.799 17.655 42.68 Example 3 29.905 18.224 39.06 Example 4 31.197 19.689 36.89 Example 5 29.220 18.715 35.95 Example 6 28.366 18.621 34.35 Comparative Example 1 29.144 21.215 27.21 Comparative Example 2 31.829 25.681 19.32 Comparative Example 3 36.935 30.257 18.08 Comparative Example 4 29.658 24.168 18.51 Comparative Example 5 31.684 28.105 11.30 Comparative Example 6 32.881 29.392 10.61 Comparative Example 7 30.370 26.321 13.33

[0141] As can be seen from the results in Table 3, the essential oil provided by this invention has a better effect on improving spots.

[0142] This invention provides, exemplarily, images showing the improvement in blemishes in three subjects after using the essential oil prepared in Example 1, as shown. Figure 1 (Left: Before use; Right: After use) Figure 2 (Left: Before use, Right: After use) and Figure 3 (Left: Before use, Right: After use) As shown in the figure, it is very clear that using the essential oil provided by this invention can significantly improve blemishes and has an excellent blemish-removing effect.

[0143] Table 4

[0144]

[0145]

[0146] As can be seen from the results in Table 4, the essential oil provided by this invention has a superior effect on improving brown spots.

[0147] This invention provides, exemplarily, images showing the improvement in brown spots in two subjects after using the essential oil prepared in Example 1, as shown. Figure 4 (Left: Before use, Right: After use) and Figure 5 (Left: Before use, Right: After use) As shown in the figure, it is very clear that using the essential oil provided by this invention can significantly improve brown spots and has an excellent spot-removing effect.

[0148] Table 5

[0149] Before use After use (8 weeks) Improvement rate (%) Example 1 28.888 4.360 84.91 Example 2 31.456 5.653 82.03 Example 3 30.657 7.006 77.15 Example 4 29.835 10.683 64.19 Example 5 29.723 10.903 63.32 Example 6 31.851 13.537 57.50 Comparative Example 1 27.195 13.865 49.02 Comparative Example 2 28.996 15.258 47.38 Comparative Example 3 30.612 19.236 37.16 Comparative Example 4 30.335 21.132 30.34 Comparative Example 5 29.391 20.951 28.72 Comparative Example 6 26.825 19.334 27.93 Comparative Example 7 29.106 22.156 23.88

[0150] As can be seen from the results in Table 5, the wrinkle-improving effect is superior when using the essential oil provided by this invention.

[0151] This invention provides, exemplarily, images showing the improvement in blemishes in three subjects after using the essential oil prepared in Example 1, as shown. Figure 6 (Left: Before use; Right: After use) Figure 7(Left: Before use, Right: After use) and Figure 8 (Left: before use, Right: after use) As shown in the figure, it is very clear that the essential oil provided by this invention has excellent wrinkle-reducing effect.

[0152] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. A whitening and anti-aging composition for use in skincare products, characterized in that, This whitening and anti-aging composition contains an active ingredient combination and a compound essential oil and plant oil combination; the mass ratio of the active ingredient combination to the compound essential oil and plant oil combination is 1:0.5-15. The active ingredient combination contains glycyrrhizin, 4-butylresorcinol, undecenoyl phenylalanine, natural bisabolol, ascorbate tetraisopalmitate and teprenone; The compound essential oil and plant oil combination contains Damask rose essential oil, magnolia essential oil, frankincense essential oil, Roman chamomile essential oil, carrot seed essential oil, passion fruit seed oil, red goji berry seed oil, rosehip oil, and sea buckthorn fruit oil. In the whitening and anti-aging composition, relative to 100 parts by weight of 4-butylresorcinol, the content of glycyrrhizin is 2-50 parts by weight, the content of undecylenoyl phenylalanine is 10-350 parts by weight, the content of natural bisabolol is 10-100 parts by weight, the content of ascorbic acid tetraisopalmitate is 50-1000 parts by weight, the content of teprenone is 5-200 parts by weight, and the content of Damask rose essential oil is 5-300 parts by weight. The content of the magnolia essential oil is 2-150 parts by weight, the content of the frankincense essential oil is 5-250 parts by weight, the content of the Roman chamomile essential oil is 2-150 parts by weight, the content of the carrot seed essential oil is 5-200 parts by weight, the content of the passion fruit seed oil is 50-500 parts by weight, the content of the red goji berry seed oil is 50-500 parts by weight, the content of the rosehip oil is 50-3000 parts by weight, and the content of the sea buckthorn fruit oil is 10-200 parts by weight. Relative to 100 parts by weight of 4-butylresorcinol, the whitening and anti-aging composition also contains 100-200 parts by weight of antioxidants; based on the total weight of the antioxidants, the antioxidants contain 1-10 wt% of dimethylmethoxybenzodihydropyranol, 5-20 wt% of reduced coenzyme Q10, and 50-85 wt% of natural tocopherol.

2. The whitening and anti-aging composition according to claim 1, characterized in that, The mass ratio of the active ingredient combination to the compound essential oil and plant oil combination is 1:4-10.

3. The whitening and anti-aging composition according to claim 1 or 2, characterized in that, In the whitening and anti-aging composition, relative to 100 parts by weight of 4-butylresorcinol, the content of glycyrrhizin is 5-30 parts by weight, the content of undecylenoyl phenylalanine is 100-300 parts by weight, the content of natural bisabolol is 20-80 parts by weight, the content of ascorbic acid tetraisopalmitate is 100-800 parts by weight, the content of teprenone is 10-150 parts by weight, and the content of Damask rose essential oil is 20-250 parts by weight. The content of the magnolia essential oil is 5-120 parts by weight, the content of the frankincense essential oil is 10-200 parts by weight, the content of the Roman chamomile essential oil is 5-120 parts by weight, the content of the carrot seed essential oil is 10-150 parts by weight, the content of the passion fruit seed oil is 100-400 parts by weight, the content of the red goji berry seed oil is 100-400 parts by weight, the content of the rosehip oil is 150-2500 parts by weight, and the content of the sea buckthorn fruit oil is 20-150 parts by weight.

4. The whitening and anti-aging composition according to claim 1 or 2, characterized in that, In addition to 100 parts by weight of 4-butylresorcinol, the compound essential oil and plant oil combination also contains 10-300 parts by weight of blood orange essential oil, 5-250 parts by weight of pink pepper essential oil, 50-1500 parts by weight of tomato seed oil, 100-1000 parts by weight of pomegranate seed oil, and 1000-4000 parts by weight of perilla seed oil.

5. The whitening and anti-aging composition according to claim 4, characterized in that, In addition to 100 parts by weight of 4-butylresorcinol, the compound essential oil and plant oil combination also contains 20-200 parts by weight of blood orange essential oil, 2-100 parts by weight of pink pepper essential oil, 5-1000 parts by weight of tomato seed oil, 350-450 parts by weight of pomegranate seed oil, and 3000-3500 parts by weight of perilla seed oil.

6. The use of the whitening and anti-aging composition according to any one of claims 1-5 in the preparation of skin care products.

7. The application according to claim 6, characterized in that, The skincare products include facial oils.

8. An essential oil, characterized in that, The essential oil contains excipients and the whitening and anti-aging composition according to any one of claims 1-5, and the total content of the whitening and anti-aging composition is not less than 50 wt%.

9. The essential oil according to claim 8, characterized in that, The excipients are selected from at least one of the following: triglyceride (ethylhexanoate), Hallstar's Sensolene Light ET, macadamia oleate, jojoba seed oil, comfrey oil, unsaponifiables of shea butter, and caprylic / capric triglyceride.

10. The essential oil according to claim 9, characterized in that, Based on the total weight of the excipients, the excipients contain 10-20 wt% triglyceride (ethylhexanoate), 1-5 wt% Hallstar's Sensolene LightET, 5-15 wt% macadamia oleate, 40-65 wt% jojoba seed oil, 1-5 wt% comfrey oil, 1-5 wt% unsaponifiables of shea butter, and 10-20 wt% caprylic / capric triglyceride.

11. A method for preparing the essential oil according to claim 9 or 10, characterized in that, The method includes: (1) The components in phase A are mixed for the first time to obtain mixture I; phase A contains caprylic / capric triglyceride, triglyceride (ethylhexanoate) and glycyrrhizin; (2) Mix phase B with the mixture I for the second time to obtain mixture II; phase B contains Hallstar's Sensolene Light ET, ethyl macadamia oleate, dimethyl methoxybenzodihydropyranol, unsaponifiables of shea butter, 4-butylresorcinol and undecenoyl phenylalanine; (3) Mix phase C with mixture II for the third time to obtain mixture III; phase C contains reduced coenzyme Q10 and natural bisabolol; (4) The D phase is mixed with the mixture III for the fourth time to obtain the mixture IV; the D phase contains a compound essential oil and plant oil combination, jojoba seed oil, ascorbate tetraisopalmitate, comfrey oil, natural tocopherol, and teprenone.

12. The method according to claim 11, characterized in that, The conditions for the first mixing include: a temperature of 60-65℃, a stirring speed of 30-80 rpm, and a time of 10-30 min; The conditions for the second mixing include: a temperature of 55-60℃, a stirring speed of 30-80 rpm, and a time of 20-40 min; The conditions for the third mixing include: a temperature of 45-50℃, a stirring speed of 30-80 rpm, and a time of 10-40 min; The conditions for the fourth mixing include: a temperature of 25-35℃, a stirring speed of 30-80 rpm, and a time of 10-40 min.

Images