A fungicidal composition for controlling crop diseases caused by fusarium

Abstract

The application discloses a bactericidal composition and application thereof in prevention and treatment of crop diseases, and belongs to the technical field of plant disease prevention and treatment. An active ingredient of the composition comprises a compound ZJS178 with a structure as shown in formula (I) and a methoxy acrylate bactericide, and the bactericidal composition has excellent prevention and treatment effects on crop diseases caused by fusarium. The compound ZJS178 and the methoxy acrylate bactericide have a synergistic effect under certain matching conditions, and can significantly improve bacteriostatic activity and prevention and treatment effects. The composition provided by the application is composed of effective ingredients with different action mechanisms, can overcome and delay drug resistance of pathogenic bacteria, is safe for crops, and meets the requirements of pesticide reduction and synergism.

Description

Technical Field

[0001] This invention relates to the field of plant disease control technology, specifically to a bactericidal composition and its application in the control of crop diseases. Background Technology

[0002] Fusarium is a globally distributed fungus that causes various plant diseases, including root rot, stem rot, stem base rot, flower rot, and ear rot, leading to crop wilting and death, affecting yield and quality, and is one of the most difficult diseases to control in production. Common diseases include rice seedling blight, wheat scab, cucurbit wilt, and root rot in various crops. *Fusarium oxysporum* is a globally distributed soil-borne pathogen with a wide host range, causing wilt in more than 100 plant species, including cucurbits, solanaceae, bananas, cotton, legumes, and flowers. Watermelon wilt caused by this fungus is a widespread and devastating soil-borne and seed-borne fungal disease. Generally, affected fields experience yield reductions of 30%–40%, with severely affected fields experiencing yield reductions of over 80%, even resulting in total crop failure. This is especially true for watermelons grown in protected cultivation facilities, where years of continuous cropping exacerbate the disease, posing a significant threat to watermelon production. In recent years, with the continuous expansion of chili pepper planting area and the increasing multiple cropping index in my country, Fusarium oxysporum, the wilt pathogen of chili peppers, has accumulated and spread in the fields year by year, leading to a decline in quality and yield. In severe cases, it can reduce yield by 70% to 80%, or even cause complete crop failure. Therefore, continuous and efficient control of crop diseases caused by Fusarium is of great significance for ensuring the effective supply and quality safety of agricultural products in my country.

[0003] Currently, due to the lack of highly resistant varieties, chemical control plays an important role in crop diseases.

[0004] Azoxystrobin, chemically known as (E)-2-{2-[6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl}-3-methoxyacrylate methyl methacrylate, belongs to the methoxyacrylate fungicide class. It inhibits mitochondrial respiration by suppressing electron transfer between fungal cytochrome bc1 cells, thus causing fungal cell death. This agent has good control effects on various crop diseases caused by pathogens of Ascomycota, Basidiomycota, Oomycota, and Deuteromycota. It is highly effective and broad-spectrum, exhibiting good activity against powdery mildew, rust, glume blight, net blotch, downy mildew, and rice blast in cereals, rice, peanuts, grapes, potatoes, fruit trees, vegetables, coffee, and lawns. It can be used for foliar spraying, seed treatment, and soil treatment.

[0005] Trifloxystrobin, chemical name: (E)-methoxyimino-{(E)-α-[1-(α,α,α-trifluorom-methyl)ethiminooxy]o-tolyl]acetic acid methyl ester, is a broad-spectrum fungicide with a long-lasting effect. It exhibits protective, curative, and eradicative activity against most diseases caused by pathogenic fungi in the Ascomycetes, Basidiomycetes, Oomycetes, and Deuteromycetes classes. It shows no cross-resistance with existing fungicides.

[0006] Fluoxastrobin, chemical name: {2-[6-(2-chlorophenoxy)-5-fluoropyrimidin-4-yloxy]phenyl}(5,6-dihydro-1,4,2-dioxazin-3-yl)methyl ketone O-methyl oxime, has broad-spectrum fungicidal activity and is highly effective against almost all fungal diseases (Ascomycetes, Basidiomycetes, Oomycetes, and Deuteromycetes), such as rust, glume, net blotch, powdery mildew, and downy mildew.

[0007] Picoxystrobin, chemical name: (E)-3-methoxy-2-{2-[6-(trifluoromethyl)-2-pyridoxymethyl]phenyl}methyl acrylate, is a broad-spectrum systemic fungicide.

[0008] Pyraclostrobin, chemical name: N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate, has protective, curative, systemic, and rain-wash-resistant properties, and has a wide range of applications.

[0009] Enoximilamide, chemical name: (E,E,E)-N-methyl-2-[((((1-methyl-3-(2,6,-dichlorophenyl)-2-propenyl)imino)oxy)methyl)phenyl]-2-methoxyiminoacetamide, has a broad fungicidal spectrum, high activity, and both preventive and curative effects. It has good control effects on a variety of plant diseases caused by flagellates, zygomycetes, ascomycetes, basidiomycetes, and deuteromycetes, and is particularly effective against powdery mildew and rust.

[0010] Long-term use of pesticides can lead to pathogen resistance, affecting their efficacy. Therefore, developing suitable pesticide combinations is of significant practical importance for the sustainable control of crop diseases. Summary of the Invention

[0011] The purpose of this invention is to provide a highly efficient, low-toxicity, and environmentally friendly bactericidal composition that is effective against crop diseases caused by Fusarium, while also delaying the development of pesticide resistance and extending the lifespan of the pesticide.

[0012] To achieve the above objectives, the present invention adopts the following technical solution:

[0013] This invention provides a bactericidal composition comprising compound ZJS178 with the structure shown in formula (I) and a methoxyacrylate bactericide.

[0014]

[0015] The aforementioned compound ZJS178 is a novel compound previously prepared by our research group. Its chemical name is ethyl 2-cyano-3-amino-3-[4-(N-ethyl-N-methylamino)phenyl]acrylate, and the synthetic route is as follows:

[0016]

[0017] This compound is a cyanoacrylate bactericide that acts on type I myosin (motor protein) of Fusarium, causing the bacteria to lose its growth momentum and leading to bacterial death. ZJS178 has a novel structure and a unique mode of action, and does not exhibit cross-resistance with methoxyacrylate bactericides.

[0018] Preferably, the mass ratio of ZJS178 to the methoxyacrylate bactericide is 60:1 to 1:60.

[0019] The methoxyacrylate fungicides include, but are not limited to: azoxystrobin, oxadiazon, fluopyram, pyraclostrobin, pyraclostrobin, difenoconazole, and acetamiprid.

[0020] More preferably, the mass ratio of ZJS178 to the methoxyacrylate bactericide is 20:1 to 1:20.

[0021] More preferably, the mass ratio of ZJS178 to the methoxyacrylate bactericide is 12:1 to 1:12.

[0022] More preferably, the mass ratio of ZJS178 to the methoxyacrylate bactericide is 6:1 to 1:6.

[0023] Most preferably, the mass ratio of ZJS178 to the methoxyacrylate bactericide is 3:1 to 1:3.

[0024] The composition provided by this invention is suitable for sterilization, and is particularly suitable for agricultural sterilization.

[0025] Preferably, the composition provided by the present invention is suitable for the prevention and control of diseases caused by Fusarium.

[0026] More preferably, the Fusarium refers to plant pathogenic Fusarium, mainly including Fusarium graminearum complex, Fusarium fujikuroi complex, Fusarium oxysporum, and Fusarium moniliforme, etc.

[0027] More preferably, the composition provided by the present invention is used to prevent and control diseases such as wilt of cucurbits, wilt of tomatoes, wilt of bananas, wilt of cotton, wilt of strawberries, bakanae disease of rice, and scab of wheat.

[0028] The bactericidal composition provided by the present invention contains 0.5 to 90% of the composition by weight of the bactericide.

[0029] More preferably, the bactericide contains 5 to 80% of the composition by weight.

[0030] The fungicide provided by the present invention may further include, in addition to the composition, an agriculturally acceptable carrier and adjuvants.

[0031] ZJS178 has a different mechanism of action than methoxyacrylate fungicides. This invention combines ZJS178 with both, resulting in a significantly higher control efficacy against Fusarium-induced diseases compared to single agents. This reduces the amount of a single agent required, helps delay the development of fungal resistance, and extends the lifespan of the fungicide.

[0032] Studies have shown that compound ZJS178, when combined with methoxyacrylate fungicides under certain mixing ratios, exhibits a synergistic effect in inhibiting the growth of Fusarium. For example, compound ZJS178, when combined with azoxystrobin at a mass ratio of 3:1 to 1:1, shows a significant synergistic effect in inhibiting the growth of Fusarium oxysporum.

[0033] Furthermore, the bactericidal composition also includes auxiliary components required for pesticide formulations, with the active ingredient comprising 0.5% to 90% of the composition by mass. These auxiliary components are commonly used carriers and adjuvants in pesticides.

[0034] According to methods known to those skilled in the art, the bactericidal composition of the present invention can be formulated into various agriculturally permissible formulations, including water-dispersible granules, suspensions, and seed treatment suspensions, in practical applications.

[0035] For water-dispersible granules, dispersants can be polycarboxylate, lignin sulfonate, or alkyl naphthalene sulfonate; wetting agents can be alkyl sulfate, alkyl sulfonate, or naphthalene sulfonate; disintegrants can be ammonium sulfate, urea, sucrose, or glucose; binders can be diatomaceous earth, corn starch, polyvinyl alcohol, or carboxymethyl cellulose; and fillers can be diatomaceous earth, kaolin, silica, light calcium carbonate, talc, attapulgite, clay, etc.

[0036] For suspension formulations, the following additives can be used: dispersants include polycarboxylate, lignin sulfonate, and alkyl naphthalene sulfonate; wetting agents include alkylphenol polyoxyethylene polyether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, and naphthalene sulfonate; thickeners include xanthan gum, polyvinyl alcohol, and bentonite; preservatives include formaldehyde, benzoic acid, and sodium benzoate; defoamers are silicone-based defoamers; and antifreeze agents include ethylene glycol, propylene glycol, glycerin, urea, and inorganic salts such as sodium chloride.

[0037] For seed treatment suspensions, the following adjuvants can be used: film-forming agents can be polyethylene glycol, polyvinyl alcohol resin, polyvinylpyrrolidone, hydroxyethyl cellulose, homopolymer vinyl acetate, sodium carboxymethyl cellulose, gum arabic, gelatin, polyvinyl alcohol, or polyacrylamide, or mixtures thereof. Technicians can adjust the proportion of homopolymer vinyl acetate according to the required viscosity of the product.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] (1) The bactericidal composition provided by the present invention has excellent control effect on diseases caused by Fusarium. The compound ZJS178 has a significant synergistic effect when combined with methoxyacrylate bactericides, which can significantly improve bactericidal activity and control effect.

[0040] (2) The composition provided by the present invention is composed of effective ingredients with different mechanisms of action, which is beneficial to overcome and delay the drug resistance of pathogens, has good safety for crops, and meets the requirements of reducing pesticide use and increasing efficiency. Detailed Implementation

[0041] The present invention will be further described below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the spirit and essence of the invention are within the scope of the invention.

[0042] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0043] Example 1: Synthesis of compound ZJS178

[0044] Step 1: Synthesis of intermediate b

[0045]

[0046] 7.0 g of starting material a (CAS No.: 67710-36-5) and 150 mL of dichloromethane were added to a reaction flask. 11.7 g of Boc anhydride was added under ice bath conditions, followed by the slow addition of 6.0 g of triethylamine and 3.3 g of DMAP. The mixture was stirred under ice bath conditions for 0.5 hours, then brought to room temperature and stirred for another 5 hours. After the reaction was complete, 1 M hydrochloric acid was added to the reaction solution, and the organic phase was separated. The organic phase was then washed once with pure water and once with saturated brine. The organic phase was dried over anhydrous Na₂SO₄ and concentrated to obtain 9.6 g of intermediate b. This crude product was directly added to the next reaction step without further purification.

[0047] Step 2: Synthesis of intermediate c

[0048]

[0049] 9.6 g of intermediate b, 120 mL of ethanol, and 24 mL of water were added to a reaction flask. The mixture was heated to 90 °C, and 3.7 g of iron powder and 12 mL of saturated ammonium chloride solution were added. The reaction was continued for 4 hours. After the reaction was completed, the mixture was filtered with diatomaceous earth. The filtrate was concentrated, and water was added. The mixture was extracted three times with ethyl acetate. The organic phases were combined, concentrated, and separated by column chromatography (eluting solvent: ethyl acetate and petroleum ether, volume ratio 1:2) to give 4.5 g of intermediate c, with a yield of 51.1%.

[0050] Step 3: Synthesis of intermediate d

[0051]

[0052] 0.19 g of sodium hydride was added to 20 mL of tetrahydrofuran under ice bath conditions, followed by 1.0 g of intermediate c. The mixture was then heated to room temperature and stirred for 30 minutes. Next, 0.74 g of iodoethane was added, and the mixture was stirred overnight at room temperature. After the reaction was complete, water was added to quench the reaction, followed by extraction three times with ethyl acetate. The organic phases were combined, concentrated, and separated by column chromatography (eluent: ethyl acetate and petroleum ether, volume ratio 1:5) to give 0.52 g of intermediate d, in 48.0% yield.

[0053] Step 4: Synthesis of intermediate e

[0054]

[0055] 0.11 g of sodium hydride was added to 10 mL of tetrahydrofuran under ice bath conditions, followed by 0.5 g of intermediate d. The mixture was then stirred for 30 minutes after reaching room temperature. Next, 0.40 g of iodomethane was added, and the mixture was stirred overnight at room temperature. After the reaction was complete, water was added to quench the reaction, followed by extraction three times with ethyl acetate. The organic phases were combined, concentrated, and separated by column chromatography (eluent: ethyl acetate and petroleum ether, volume ratio 1:5) to give 0.34 g of intermediate e, in 65.5% yield.

[0056] Step 5: Synthesis of compound ZJS178

[0057]

[0058] 0.34 g of intermediate e and 10 mL of tetrahydrofuran were added to a reaction flask. Hydrogen chloride gas was produced by adding sodium chloride dropwise with concentrated sulfuric acid and slowly passed into the reaction solution. After 1 hour, the gas flow was stopped, and the mixture was stirred overnight at room temperature. After the reaction was complete, saturated sodium bicarbonate aqueous solution was added, and the mixture was extracted twice with ethyl acetate. The organic phases were combined, concentrated, and separated by column chromatography (eluent: ethyl acetate and petroleum ether, volume ratio 1:2) to give 0.22 g of compound ZJS178, with a yield of 93.2%.

[0059] Example 2: Indoor toxicity test of ZJS178 combined with azoxystrobin against Fusarium wilt pathogen of tomato

[0060] Experimental subjects: Fusarium oxysporum f. sp. lycopersici, the pathogen of tomato wilt, was isolated from diseased tomatoes. After morphological and pathogenic identification, the test strains were preserved in our laboratory for future use.

[0061] Test reagents: ZJS178 was synthesized from Example 1; azoxystrobin was provided by Zhejiang Provincial Chemical Research Institute.

[0062] Experimental Methods: The mycelial growth rate method was referenced in the "Agricultural Industry Standard of the People's Republic of China NY / T 1156.2-2006". Five dosage treatments were set up for each agent based on the content of the active ingredient, with inhibition rates against pathogen growth ranging from 10% to 90%. The above-mentioned *Fusarium wilt* pathogen was inoculated onto PDA medium, and 50 μg / ml salicylic acid (SHAM) was added to the medium to inhibit the pathogen's bypass respiration. When the colonies covered 2 / 3 of the petri dish, mycelial blocks were formed at the edge of the colonies using a 5 mm diameter punch. These mycelial blocks were then transferred to the center of a pre-prepared drug-containing medium plate using an inoculation needle and incubated at 25°C for 3 days. The colony diameter for each treatment was measured using calipers using the cross-sectional method to determine the corrected inhibition percentage. The EC50 value of each agent was then calculated using linear regression analysis between the probability value of the inhibition rate and the logarithm of the series concentrations. Each treatment was repeated four times. ZJS178 was used as the standard agent, and the co-toxicity coefficient (CTC) was calculated using the following formula.

[0063] Actual Toxicity Index (ATI) = (EC50 of the standard reagent / EC50 of the test reagent) × 100;

[0064] Theoretical Toxicity Index (TTI) = Toxicity Index of Standard Agent × Percentage of Standard Agent in Mixture + Toxicity Index of Test Agent × Percentage of Test Agent in Mixture;

[0065] Cotoxicity coefficient (CTC) = (ATI / TTI) × 100%;

[0066] A CTC value less than 80 indicates that the combination of drugs has an antagonistic effect; a value between 80 and 120 indicates an additive effect; and a value greater than 120 indicates a synergistic effect.

[0067] The test results are shown in Table 1.

[0068] Table 1: Indoor toxicity test results of ZJS178 combined with azoxystrobin against Fusarium wilt pathogen of tomato.

[0069]

[0070]

[0071] As shown in Table 1, when the mass percentage of ZJS178 to azoxystrobin is 4:1 to 1:6, the antibacterial effect against the growth of Fusarium wilt of tomato is significantly enhanced; other ratios have an additive effect.

[0072] Example 3: Indoor toxicity test of ZJS178 combined with pyraclostrobin against rice bakanae disease

[0073] Test subject: Rice bakanae disease (Fusarium moniliforme), preserved in the strain room of this laboratory. Bioassay method is as described in Example 2.

[0074] Table 2: Indoor toxicity test results of ZJS178 combined with pyraclostrobin against rice bakanae disease.

[0075] medicine <![CDATA[EC 50 (μg / mL)]]> ATI TTI Cotoxicity coefficient (CTC) ZJS178(A) 0.6781 100.00 / / Pyraclostrobin (B) 4.9852 13.61 / / A+B(1:20) 3.4121 19.89 17.72 112.19 A+B(1:15) 3.0678 22.12 19.01 116.35 A+B(1:12) 2.6822 25.30 20.26 124.89 A+B(1:8) 2.2455 30.22 23.21 130.19 A+B(1:6) 1.9855 34.17 25.95 131.68 A+B(1:4) 1.5321 44.29 30.89 143.37 A+B(1:3) 1.3025 52.09 35.21 147.96 A+B(1:2) 1.1034 61.49 42.41 145.00 A+B(1:1) 0.8322 81.53 56.81 143.53 A+B(2:1) 0.6215 109.17 71.20 153.32 A+B(3:1) 0.578 117.39 78.40 149.72 A+B(4:1) 0.5899 115.02 82.72 139.04 A+B(6:1) 0.6021 112.69 87.66 128.55 A+B(8:1) 0.6107 111.10 90.40 122.90 A+B(12:1) 0.6124 110.79 93.35 118.68 A+B(15:1) 0.6278 108.08 94.60 114.24 A+B(20:1) 0.6488 104.58 95.89 109.06

[0076] As shown in Table 2, when the mass percentage of ZJS178 to pyraclostrobin is 1:12 to 8:1, the inhibitory effect on the growth of rice bakanae disease fungus is significantly enhanced; other ratios have an additive effect.

[0077] Example 4: Field efficacy trial

[0078] I. Formulation

[0079] All percentages in the formulation are by mass.

[0080] (1) 40% ZJS178·pyraclostrobin suspension

[0081] Weigh out 30% ZJS178, 10% azoxystrobin, 2% NNO, 2% TERSPERSE 2500, 1% emulsifier T-60, 3% agricultural emulsion 700#, 0.1% xanthan gum, 3% silica, 5% propylene glycol, 0.5% formaldehyde, and 0.5% silicone defoamer, and add deionized water to 100% by weight.

[0082] According to the formula ratio, using water as the medium, the active ingredient, dispersant, suspending agent and antifreeze are added to the mixing tank and mixed evenly. The mixture is then dispersed for 30 minutes by ball mill or high-speed shearing, and finally ground with a sand mill to make a suspension.

[0083] (2) 24% ZJS178·pyraclostrobin suspension

[0084] Weigh out 16% ZJS178, 8% pyraclostrobin, 2% TERSPERSE 2500, 3% TERSPERSE 2425, 0.2% xanthan gum, 3% silica, 5% ethylene glycol, 0.3% benzoic acid, and 0.5% silicone defoamer (trade name: S-29, produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight.

[0085] According to the formula ratio, using water as the medium, the active ingredient, dispersant, suspending agent and antifreeze are added to the mixing tank and mixed evenly. The mixture is then dispersed for 30 minutes by ball mill or high-speed shearing, and finally ground with a sand mill to make a suspension.

[0086] II. Field Trials

[0087] Field efficacy trial for controlling watermelon wilt

[0088] The control of watermelon wilt was carried out in accordance with the provisions of "GB-T 17980.113-2004 Field Efficacy Test Guidelines for Pesticides (II) - Control of Cucurbit Wilt by Fungicides". The test site was located in Dongpu Town, Shaoxing City. The soil type of the test site was loam, with medium fertility and neutral pH. The variety was Zhemi No. 8. After the watermelon seedlings were transplanted, the roots were drenched with pesticide solution, and the control pesticide was applied by broadcasting. The types and amounts of pesticides are shown in Table 3. One week after the first application, the roots were drenched again. Fourteen days after the pesticide treatment, all plants in the plot were investigated to see if they had typical symptoms of wilt. The number of plants with wilt and the total number of plants investigated were recorded. Based on the investigation results, the disease incidence and control efficacy were calculated according to the formulas (1) and (2) below. The experimental data were statistically analyzed using Duncan's New Multiple Range Method (DMRT).

[0089]

[0090]

[0091] The experimental results are shown in Table 3.

[0092] Table 3: Field efficacy test results of ZJS178 and azoxystrobin compound against watermelon wilt disease

[0093]

[0094] *Different lowercase letters in the same column indicate significant differences at the P<0.05 level.

[0095] Table 3 shows that, under the same effective dosage, the combination of ZJS178 and pyraclostrobin has excellent control effect against watermelon wilt, with significantly higher efficacy than the single agent. The tested agents are safe for the tested crops.

[0096] Field efficacy trial for controlling rice bakanae disease

[0097] The control of rice bakanae disease was conducted in accordance with the provisions of "GB-T 17980.104-2004 Field Efficacy Test Guidelines for Pesticides (II) - Control of Rice Bakanae Disease with Fungicides". Five samples were taken from each plot, with 100 plants investigated at each point. The total number of plants and the number of diseased plants were recorded. Before heading, five random samples were taken from each plot, with 20 clumps at each point. The experimental site was located in Dongpu Town, Shaoxing City. The pesticide solution was diluted to a certain ratio and the seeds were soaked for 72 hours. The soil type of the experimental site was loam, with moderate fertility and a neutral pH. The rice variety was Zhongzao 39 (a susceptible variety). Nutrient soil was used for seedling raising, and the seedling raising process strictly followed the machine transplanting seedling raising procedure. The seed quantity and soil fertility were consistent for all treatments. Each treatment had 20 seedling trays, with 150g of seeds used per tray. The incidence of bakanae disease was investigated 1 day before rice transplanting and before heading. Efficacy calculation method:

[0098] Based on the survey results, the disease index and prevention efficacy were calculated according to formulas (1) and (2) below. The experimental data were statistically analyzed using the Duncan Multiple Range Test (DMRT).

[0099]

[0100]

[0101] Table 4: Results of field efficacy trials for controlling rice bakanae disease

[0102]

[0103] *Different lowercase letters in the same column indicate significant differences at the P<0.05 level.

[0104] Table 4 shows that 24% ZJS178·pyraclostrobin suspension concentrate, when diluted 3000–2000 times, has a good effect on rice bakanae disease during seed soaking, with a control efficacy of 89.8%–96.2%, significantly better than single-agent treatment. The tested agent is safe for the tested crops.

[0105] In summary, the results of indoor bioassays and field efficacy trials indicate that the composition of this invention has a reasonable composition, providing both therapeutic and protective effects, good bactericidal efficacy, reduced application frequency, and low cost. Furthermore, its activity and bactericidal effect are not simply the sum of the activities of its components, but rather exhibit a significant synergistic effect. The composition consists of active ingredients with different mechanisms of action, increasing the number of action sites, which helps overcome and delay the development of pathogen resistance. It has no significant adverse effects on the tested crops; leaf color and growth remained normal, demonstrating good safety and meeting the safety requirements for pesticide formulations. This invention has excellent control efficacy against diseases caused by Fusarium.

Claims

1. A bactericidal composition, characterized in that: The active components of the bactericidal composition include compound ZJS178 with the structure shown in formula (I) and a methoxyacrylate bactericide: The methoxyacrylate fungicide is azoxystrobin or pyraclostrobin; The mass ratio of ZJS178 to azoxystrobin in the bactericidal composition is 4:1 to 1:

6. The mass ratio of ZJS178 to pyraclostrobin in the bactericidal composition is 1:12 to 8:

1.

2. The bactericidal composition according to claim 1, characterized in that: The mass ratio of ZJS178 to methoxyacrylate bactericide in the bactericidal composition is 3:1 to 1:

3.

3. The bactericidal composition according to claim 1 or 2, characterized in that: The bactericidal composition also includes auxiliary components required for pesticide formulations, and the mass percentage of the active ingredient in the composition is 0.5% to 90%.

4. The application of the fungicidal composition according to any one of claims 1-3 in the prevention and control of crop diseases caused by Fusarium, characterized in that: The Fusarium species mentioned are Fusarium oxysporum and Fusarium moniliforme.

5. The application as described in claim 4, characterized in that: The crop diseases mentioned include: wilt of cucurbits, wilt of tomatoes, wilt of bananas, wilt of cotton, wilt of strawberries, and bakanae disease of rice.

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