An air inlet structure for a plant tissue culture vessel and the plant tissue culture vessel

Abstract

This invention discloses an air intake structure for a plant tissue culture vessel and the vessel itself. The air intake structure includes an air intake section, an aeration section, and a conduit connecting the air intake section and the aeration section. The aeration section has a cavity communicating with the conduit, and the cavity wall has a plurality of densely distributed micropores communicating with the inside and outside of the cavity. In this invention, by densely opening a large number of micropores on the side wall of the aeration section, and ensuring that all micropores communicate with the cavity at the center of the aeration section, the gas entering the cavity of the aeration section through the air guide can be simultaneously sprayed into the solution from all the micropores. This simultaneously forms a large number of small bubbles in the solution. The small bubbles move outward from the outer surface of the aeration section and float upward, preventing them from coalescing into large bubbles, thus improving the contact and dissolution rate between the gas and the solution. Furthermore, the dispersed floating movement of a large number of small bubbles in the solution promotes the circulation of the solution, further improving the dissolved oxygen effect.

Description

Technical Field

[0001] This invention belongs to the technical field of plant tissue culture devices, specifically, it relates to an air intake structure and a plant tissue culture vessel. Background Technology

[0002] In the existing technology, scientists have developed a method and cultivation device for large-scale cultivation using isolated plant tissues or cells. This involves separating plant organs such as roots, stems, and leaves, placing them in a nutrient-rich culture medium, and providing suitable environmental conditions such as temperature and oxygen to induce callus formation, adventitious buds, and adventitious roots. Finally, these callus tissues, adventitious buds, and adventitious roots are used as seeds for cultivating plants, and then placed in the cultivation device for further cultivation.

[0003] Existing plant tissue culture tanks typically place the aeration device for increasing dissolved oxygen at the bottom of the tank. This allows the gas sprayed from the aeration device to rise from the bottom to the top of the tank, ensuring sufficient contact with the culture medium to increase dissolved oxygen levels. However, because the number of air outlets on the aeration device is small and the orifice diameter is too large, the gas sprayed from the aeration device easily forms large bubbles in the solution, failing to effectively disperse oxygen into the culture medium.

[0004] Chinese utility model patent application number 201620182727.0 discloses a novel fermenter for culturing microorganisms. It includes a tank body, a sealing cover disposed on the top of the tank body, an aeration device, and a computer. The tank body is wrapped with a jacket, and a straight pipe is disposed on the jacket. The aeration device is disposed inside the tank body and consists of an air inlet pipe and an aeration belt. The air inlet pipe is disposed on the tank body, and multiple nozzles are disposed on the air inlet pipe along the length of the air inlet pipe. Each nozzle is connected to an aeration belt, and multiple micropores are distributed on each aeration belt. A temperature sensor is disposed below the air inlet pipe and is connected to the computer.

[0005] This invention evenly disperses the micropores for air output on multiple aeration zones in the aeration device, thereby preventing the formation of large bubbles in the culture medium from the gas sprayed from the aeration device and improving the dissolved oxygen effect. However, the aeration device has a large number of aeration zones, which, when suspended in the culture medium, easily adsorb adventitious roots, affecting the circulation and flow of adventitious roots, and may even lead to the decay of adventitious roots.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] The purpose of this invention is to provide an air intake structure for a plant tissue culture tank, so as to reduce the volume of bubbles, increase the number of bubbles, and greatly reduce the volume of the aeration device inside the tank by optimizing the microporous structure of the aeration device.

[0008] The present invention also aims to provide a plant tissue culture vessel with the above-mentioned air intake structure, so as to increase the dissolved oxygen content of the culture solution in the vessel, better promote the growth of adventitious roots, and improve the quality and yield of adventitious roots.

[0009] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows:

[0010] An air intake structure for a plant tissue culture vessel includes an air intake section, an aeration section, and a conduit connecting the air intake section and the aeration section. The aeration section has a cavity communicating with the conduit, and the cavity wall has a plurality of densely distributed micropores communicating with the inside and outside of the cavity.

[0011] Furthermore, the aeration section is formed by stacking and sintering a large number of small particles with a diameter of less than 1 mm, and the gaps between different small particles form airflow channels extending from the inner surface of the cavity wall to the outer surface of the cavity wall.

[0012] Furthermore, one end of the aeration section is connected to the conduit and extends in a straight line away from the conduit to form a long column shape, and the aeration section is arranged to coincide with the central axis of the conduit.

[0013] Furthermore, the conduit is provided with a seat plate, the aeration section is disposed on one side of the seat plate, the conduit passes through the center of the seat plate and is connected to the air inlet section disposed on the other side of the seat plate.

[0014] Furthermore, the conduit is provided with a long straight air inlet section, which extends at least a certain length to both sides of the seat plate. The aeration section and the air inlet section are respectively connected to the ends of the air inlet sections that extend out of the side of the seat plate.

[0015] Furthermore, the conduit is also provided with an air outlet section that is angled to the air inlet section, with one end of the air outlet section connected to the air inlet section and the other end connected to the aeration section.

[0016] Furthermore, the duct also has a transition section connecting the air inlet section and the air outlet section. The transition section is parallel to the seat plate, and the air outlet section is inclinedly connected to the transition section and extends away from the seat plate.

[0017] Furthermore, the air intake is provided with a quick-release connector, which is conical in shape and its bottom surface coincides with the air inlet end face of the duct. The conical surface of the quick-release connector faces the seat plate and gradually tapers to the outer circumference of the duct.

[0018] Furthermore, the quick-release connector is provided with a sealing groove that is recessed inward from the bottom and forms a notch on the bottom surface, and the sealing groove is arranged around the outer periphery of the air inlet of the duct.

[0019] The present invention also provides a plant tissue culture vessel having any of the above-mentioned air intake structures.

[0020] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0021] 1. By densely opening a large number of micropores on the side wall of the aeration section and connecting all the micropores to the cavity in the center of the aeration section, the gas entering the cavity of the aeration section through the air guide pipe can be sprayed into the solution from all the micropores at the same time. This creates a large number of small bubbles in the solution. The small bubbles move outward from the outer surface of the aeration section and float upward, preventing the small bubbles from agglomerating into large bubbles. This improves the contact and dissolution rate between the gas and the solution. Furthermore, the dispersed floating movement of a large number of small bubbles in the solution promotes the circulation of the solution and further improves the oxygenation effect.

[0022] 2. The aeration section is set by sintering and bonding small particles, which makes the micropores formed on the aeration section more evenly distributed, thereby better decomposing air into small bubbles that dissolve in the culture medium, which helps to increase the solubility of air in the culture medium.

[0023] 3. Designing the aeration section as a long column and extending it outward from the end of the conduit reduces the diameter of the opening on the culture tank and increases the contact area between the air inlet structure and the culture medium, which is beneficial for fully dispersing and dissolving air in the culture medium.

[0024] 4. The optimized conduit contains multiple straight sections with adjacent straight sections connected by bends. This allows the aeration section to be positioned in more locations within the culture tank when the air inlet structure is installed, increasing the range of positions where the aeration section can hover within the culture tank. This facilitates the full diffusion of air bubbles from all sides of the aeration section into the culture medium.

[0025] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0026] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0027] Figure 1 This is a schematic diagram of the air intake structure of a plant tissue culture vessel according to the present invention;

[0028] Figure 2 This is a cross-sectional schematic diagram of an air intake structure according to the present invention;

[0029] Figure 3 This is a schematic diagram of the air intake structure installation assembly of a plant tissue culture tank according to the present invention.

[0030] Among them: 10. Plant tissue culture tank; 11. Tank wall; 40. Air intake structure; 41. Air intake section; 42. Aeration section; 421. Cavity; 43. Conduit; 431. Air intake section; 432. Air outlet section; 433. Transition section; 44. Seat plate; 45. Quick-release connector; 451. Sealing groove; 51. Interface; 52. Overlapping component; 521. Through hole; 53. Hub kit; 531. Support ring; 532. Groove; 533. Connecting hole; 54. Sealing ring.

[0031] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0033] In the description of this invention, it should be noted that the terms "inner" and "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0034] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," "contact," and "communication" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0035] In this invention, plant tissues include callus, adventitious buds, adventitious roots, etc., induced from isolated plant tissues or cells.

[0036] A culture device for large-scale cultivation of adventitious roots is provided with at least two plant tissue culture tanks of different volumes.

[0037] Take a culture device with two plant tissue culture vessels as an example. For ease of distinction, the smaller plant tissue culture vessel is called an adventitious root seed vessel, and the larger plant tissue culture vessel is called an adventitious root fermentation vessel.

[0038] The plant tissue culture jars are all made of stainless steel. The height-to-diameter ratio of the jars is 1:1. The adventitious root seed jars have a volume of 200L, while the adventitious root fermentation jars have volumes of 1000L and 1500L.

[0039] Plant tissue culture vessels contain culture medium, and adventitious roots grow much faster in plant tissue culture vessels than native roots.

[0040] Example 1

[0041] like Figure 1 As shown in the embodiment of the present invention, an air intake structure 40 is provided on a plant tissue culture tank 10. The air intake structure 40 includes an air intake section 41, an aeration section 42, and a conduit 43 connecting the air intake section 41 and the aeration section 42. The conduit 43 is a straight rod, and the air intake section 41 and the aeration section 42 are respectively connected to the two ends of the conduit 43.

[0042] Specifically, the aeration section 42 has a cavity 421 at its center. The cavity 421 is connected to the conduit 43, and the cavity wall of the cavity 421 has micropores that connect from the outside of the aeration section 42 to the inside of the cavity 421. A large number of micropores are densely and evenly distributed throughout the cavity wall.

[0043] The aeration section 42 can be made of ceramic material or polymer material.

[0044] Preferably, the aeration section 42 is made of titanium alloy material, so that adventitious roots cannot adhere to the outer surface of the aeration section 42, thereby avoiding the accumulation and decay of adventitious roots.

[0045] In this example, by densely opening a large number of micropores on the side wall of the aeration section 42 and connecting all the micropores to the cavity 421 in the center of the aeration section 42, the gas entering the cavity 421 of the aeration section 42 through the air guide pipe can be sprayed into the solution from all the micropores at the same time, thereby forming a large number of small bubbles in the solution. The small bubbles move outward from the outer surface of the aeration section 42 and float upward, avoiding the small bubbles from agglomerating into large bubbles, which improves the contact and dissolution rate between the gas and the solution. Furthermore, the dispersed floating movement of a large number of small bubbles in the solution promotes the circulation of the solution and further improves the oxygenation effect.

[0046] like Figure 2As shown, in another embodiment of the present invention, an aeration section 42 of an air intake structure 40 is described. To reduce the time required to create micropores in the aeration section 42 and to ensure a more uniform distribution of micropores in the aeration section 42, air can be better decomposed into small bubbles dissolved in the culture medium. The aeration section 42 is constructed by stacking and sintering a large number of small particles with a diameter less than 1 mm together to form a shell with a central cavity 421.

[0047] Furthermore, the small particles can be ceramic materials, polymer materials, or metal alloy materials.

[0048] Specifically, the small particles are spherical. A large number of small particles are closely packed together, with gaps between adjacent particles. These gaps are arranged sequentially and connected along the radial direction of the aeration section 42, forming micropores extending from the inner surface of the cavity wall to the outer surface. Thus, the outer surface of the aeration section 42 is covered with micropores, similar to the mesh of a sieve.

[0049] Preferably, the small particles are made of titanium alloy material, which can prevent adventitious roots from adhering to the outer surface of the aeration section 42.

[0050] In this embodiment, the aeration section 42 is set by sintering and bonding small particles, so that the micropores formed on the aeration section 42 are more evenly distributed, thereby better decomposing air into small bubbles that dissolve in the culture medium, which is beneficial to increasing the solubility of air in the culture medium.

[0051] like Figure 2 As shown, in another embodiment of the present invention, an air intake structure 40 is introduced that can be more easily installed on a culture tank.

[0052] Specifically, the aeration section 42 of the air intake mechanism is elongated cylindrical, with one end of the aeration section 42 connected to the conduit 43. The central axis of the aeration section 42 coincides with the central axis of the end of the conduit 43 and extends in a straight line away from the conduit 43. At the same time, the conduit 43 is long and straight, so that the central axes of the aeration section 42 and the conduit 43 coincide.

[0053] In this embodiment, the aeration section 42 is designed as a long column and extends outward from the end of the conduit 43. This reduces the diameter of the opening on the culture tank and increases the contact area between the air inlet structure 40 and the culture liquid, which is beneficial for fully dispersing and dissolving air in the culture liquid.

[0054] like Figure 2 As shown, in another embodiment of the present invention, an air intake structure 40 that can be more easily fixed is introduced. A seat plate 44 provided on the air intake structure 40 is located between the air intake section 41 and the aeration section 42, separating the air intake section 41 and the aeration section 42.

[0055] Specifically, the seat plate 44 is connected to the conduit 43 and extends radially outward along the conduit 43, forming a shape in which the conduit 43 passes vertically through the center of the seat plate 44. In particular, the seat plate 44 is located between the two ends of the conduit 43, the aeration section 42 is distributed on one side of the seat plate 44, and the air inlet section 41 is provided on the other side of the seat plate 44.

[0056] Preferably, the seat plate 44 is configured as a circular flat plate, so that the air intake structure 40 can rotate around the duct 43 during installation.

[0057] In this embodiment, a seat plate 44 is provided on the air intake structure 40, which makes it easier to install and connect the air intake structure 40 to the culture tank, and also allows the air intake structure 40 to rotate around the conduit 43, reducing the difficulty of assembly work.

[0058] like Figure 2 As shown, in another embodiment of the present invention, an air intake structure 40 with better air intake performance is introduced. The conduit 43 of the air intake structure 40 is optimized and adjusted to improve air dissolution when it enters the culture medium.

[0059] Specifically, the duct 43 includes a long, straight air inlet section 431. The air inlet section 431 extends at least a certain length to both sides of the seat plate 44, thereby facilitating better connection and fixation of the aeration section 42 and the air inlet section 41 to the duct 43.

[0060] The aeration section 42 and the air intake section 41 are respectively connected to the ends of the air intake section 431 that extend out of the side of the seat plate 44, thereby separating the aeration section 42 and the air intake section 41 on both sides of the seat plate 44.

[0061] In another embodiment of the present invention, in order to increase the position of the aeration section 42 in the culture tank after it is installed, the conduit 43 is further provided with an air outlet section 432 that is set at an angle to the air inlet section 431.

[0062] One end of the air outlet section 432 is connected to the air inlet section 431, and the other end is connected to the aeration section 42. In particular, the included angle between the air outlet section 432 and the air inlet section 431 is greater than 90°.

[0063] In another embodiment, in order to further widen the position range of the aeration section 42 in the culture tank in conjunction with the seat plate 44, the conduit 43 is also provided with a transition section 433 connecting the air inlet section 431 and the air outlet section 432.

[0064] Specifically, a transition section 433 is provided parallel to the seat plate 44. One end of the transition section 433 is connected to the air intake section 431, and the other end is connected to the air outlet section 432. The air outlet section 432 is inclined to the transition section 433 and extends from the transition section 433 in a direction away from the seat plate 44.

[0065] Preferably, the total length of the aeration section 42, the air outlet section 432, and the transition section 433 connected together is at least greater than the radius of the cross-section of the culture tank. Furthermore, the length of the aeration section 42 is greater than the sum of the lengths of the air outlet section 432 and the transition section 433.

[0066] In this embodiment, the optimized conduit 43 includes multiple straight sections with adjacent straight sections connected by bends. This allows the air intake structure 40 to be rotated when installed in the culture tank, ensuring that the aeration section 42 can be positioned in more locations within the culture tank. This increases the range of positions where the aeration section 42 can be suspended within the culture tank, which is beneficial for bubbles to diffuse fully from the sides of the aeration section 42 into the culture medium.

[0067] like Figure 1 , Figure 2 As shown, in another embodiment of the present invention, an air intake structure 40 capable of quick connection is described. The air intake portion 41 of the air intake structure 40 includes a quick-release connector 45.

[0068] Specifically, a quick-release connector 45 is located at the end of the duct 43 in the air intake section 41. The quick-release connector 45 is conical in shape. Furthermore, the conical bottom surface of the quick-release connector 45 coincides with the air inlet end face of the duct 43. The air inlet of the duct 43 is located at the center of the bottom surface. The conical surface of the quick-release connector 45 faces the seat plate 44 and gradually tapers to the outer peripheral surface of the duct 43.

[0069] like Figure 2 As shown, in another embodiment of the present invention, a quick-release connector 45 having a sealed air intake structure 40 is described.

[0070] The quick-release connector 45 has a sealing groove 451 on its conical bottom surface. The sealing groove 451 is recessed from the bottom surface into the quick-release connector 45 and forms a notch on the bottom surface. In particular, the sealing groove 451 surrounds the outer periphery of the air inlet of the duct 43.

[0071] like Figure 3 As shown, the present invention also provides a plant tissue culture vessel 10 having any of the above-described air intake structures 40. The bottom of the plant tissue culture vessel 10 is configured as an inverted cone shape. An interface 51 for connecting the air intake structure 40 is provided on the side wall of the cone shape. The air intake structure 40 is connected to the interface 51, and its aeration part 42 is located in the cavity 421 of the plant tissue culture vessel 10, while the air intake part 41 extends out of the outside of the plant tissue culture vessel 10.

[0072] In this embodiment, the plant tissue culture tank 10, by setting the above-mentioned air intake structure 40, can make air form a large number of small bubbles in the solution when cultivating adventitious roots. The small bubbles move outward and upward from the aeration section 42, promoting the circulation of nutrient solution. Furthermore, the large number of small bubbles dispersed in the solution further improves the contact and solubility of gas and nutrient solution, providing stable conditions for the proliferation of adventitious roots, which is beneficial to improving the cultivation efficiency and quality of adventitious roots.

[0073] Example 2

[0074] like Figure 3 As shown in the embodiment of the present invention, an air inlet structure 40 mounting assembly for a plant tissue culture vessel 10 is introduced. This mounting assembly makes the connection process between the air inlet structure 40 and the plant tissue culture vessel 10 more convenient.

[0075] The air intake structure 40 includes an air intake section 41, an aeration section 42, and a conduit 43 connecting the air intake section 41 and the aeration section 42. The conduit 43 is a straight rod or a multi-section straight rod with bends, and the air intake section 41 and the aeration section 42 are respectively connected to the two ends of the conduit 43.

[0076] Specifically, the air intake structure 40 mounting assembly includes an interface 51 formed on the wall 11 of the plant tissue culture tank 10, and a seat plate 44 that can be embedded in the interface 51. The seat plate 44 is sleeved on the outer periphery of the conduit 43 and its outer periphery edge is connected to the edge of the interface 51, separating the aeration part 42 and the air intake part 41 inside and outside the plant tissue culture tank 10.

[0077] Preferably, the outer peripheral edge of the seat plate 44 is provided with external threads, and the corresponding edge of the interface 51 is provided with internal threads. The seat plate 44 and the interface 51 are connected together by the engagement of the internal and external threads.

[0078] In this embodiment, the mounting assembly includes a base plate 44 and an interface 51. The air intake structure 40 is fixedly connected to the base plate 44. By rotating the base plate 44 relative to the interface 51 of the culture tank, the relative position of the aeration section 42 of the air intake structure 40 in the culture tank can be adjusted. This allows the air intake structure 40 to have more relative positions and postures relative to the culture tank, improving the flexibility of the installation and setting of the air intake structure 40, and also helping to fully utilize the aeration function of the air intake structure 40 in the culture tank.

[0079] like Figure 3 As shown, in one embodiment of the present invention, an air intake structure 40 mounting assembly is described, which can rotate at any angle around its own central axis when connected to a culture tank. This mounting assembly includes an annular stacked member 52.

[0080] When the air intake structure 40 is fixedly installed, the stacked component 52 and the interface 51 on the culture tank are connected at their end faces and the seat plate 44 is sandwiched between them. Thus, when the stacked component 52 and the interface 51 are not tightly attached, the seat plate 44 can be rotated to adjust the position and orientation of the air intake structure 40 relative to the culture tank.

[0081] Specifically, the stacking member 52 is configured as an annular structure with a central annular hole. The stacking member 52 is sleeved with the base plate 44 and its annular hole edge abuts against the outer surface of the base plate 44 facing the plant tissue culture vessel 10, for clamping the air intake structure 40 onto the plant tissue culture vessel 10.

[0082] In this embodiment, by setting the stacking member 52, the base plate 44 can rotate at any angle relative to the interface 51, thereby causing a large number of small bubbles ejected from the air intake structure 40 to disperse in the solution, which is beneficial to promote the circulation of the solution and improve the dissolved oxygen effect. This can better promote the circulation of the culture medium in the culture tank, avoid the adventitious roots from tangling and clumping, and thus promote the faster growth of the adventitious roots.

[0083] like Figure 3 As shown, in one embodiment of the present invention, a mounting assembly is provided that can more securely clamp and fix the air intake structure 40.

[0084] Specifically, the mounting assembly also includes a hub assembly 53 with a cylindrical outer peripheral surface. The hub assembly 53 is nested in the interface 51 and connected to the interface 51 with its outer peripheral surface. In particular, the axis of the hub assembly 53 is perpendicular to the surface of the culture tank wall 11 and protrudes axially towards the inner and outer sides of the culture tank wall 11.

[0085] Meanwhile, the inner circumferential surface of the wheel hub assembly 53, which forms a central hole, is connected to the seat plate 44.

[0086] In this embodiment, the hub kit 53 protrudes from the culture tank wall 11, increasing the width of the contact surface between the seat plate 44 and the hub kit 53. Compared with the direct connection between the seat plate 44 and the tank wall 11, the sealing performance is higher and the connection strength is greater.

[0087] like Figure 3 As shown, in one embodiment of the present invention, an optimized and improved wheel hub kit 53 capable of covering the seat plate 44 is introduced.

[0088] Specifically, the hub assembly 53 has a support ring 531 at one end facing the inner wall 11 of the plant tissue culture tank 10. The support ring 531 extends radially from the inner circumference of the hub assembly 53 toward the central axis of the hub assembly 53 and forms a through hole 521 through which the aeration section 42 can pass.

[0089] The seat plate 44 is fitted onto the inner circumferential surface of the hub assembly 53 from the outside of the wall 11 of the plant tissue culture tank 10, and the seat plate 44 abuts against the support ring 531.

[0090] In this embodiment, the support ring 531 ensures that the seat plate 44 can withstand greater clamping force when fitted into the hub assembly 53, preventing the seat plate 44 from detaching from the hub assembly 53 and falling into the plant tissue culture tank 10 under excessive pressure. Therefore, the support ring 531 on the hub assembly 53 improves both the stability and sealing of the intake structure 40 mounting assembly.

[0091] like Figure 3 As shown, in one embodiment of the present invention, an intake structure 40 mounting assembly with improved sealing is provided. The mounting assembly of the intake structure 40 further includes a sealing ring 54 for filling gaps between contact surfaces.

[0092] Specifically, the sealing ring 54 is made of rubber, which is elastic and has good extensibility. Furthermore, the sealing ring 54 is disposed between the seat plate 44 and the support ring 531, and is held between the end face of the seat plate 44 facing the inside of the plant tissue culture jar 10 and the end face of the support ring 531.

[0093] Specifically, when the seat plate 44 and the support ring 531 clamp the sealing ring 54, the thickness of the sealing ring 54 is reduced, so that the protrusions and depressions on the end faces of the seat plate 44 and the support ring 531 that are close to each other are filled by the sealing ring 54, thereby improving the sealing performance of the air intake structure 40 mounting assembly.

[0094] like Figure 3 As shown, in one embodiment of the present invention, an intake structure 40 mounting assembly that can better define the sealing ring 54 is described.

[0095] Specifically, the support ring 531 is disposed at the end of the wheel hub assembly 53 located inside the tank wall 11. The through hole 521 on the support ring 531 is aligned with the central hole of the wheel hub assembly 53 along its central axis. Furthermore, one end face of the support ring 531 is flush with the end face of the wheel hub assembly 53, and the other end face is located inside the central hole of the wheel hub assembly 53.

[0096] Specifically, a groove 532 is formed on the end face of the support ring 531 located within the central hole of the hub assembly 53. The opening of the groove 532 faces the outer side of the wall 11 of the plant tissue culture tank 10. The sealing ring 54 is embedded in the groove 532 and protrudes from the end face of the support ring 531. When the air intake structure 40 is installed and fixed, the seat plate 44 compresses the sealing ring 54, causing the sealing ring 54 to deform and fill the groove 532.

[0097] In this embodiment, by providing a groove 532 on the end face of the support ring 531 and fixing the sealing ring 54 in the groove 532, the sealing ring 54 can be rigidly supported when it is squeezed and deformed, avoiding damage by huge pressure, thus improving the service life of the sealing ring 54. At the same time, it can also prevent the sealing ring 54 from sliding out of the set position after being squeezed, causing the sealing failure problem.

[0098] like Figure 3 As shown, in one embodiment of the present invention, a more flexible intake structure 40 mounting assembly is introduced. The mounting assembly has a seat plate 44 that fits into the wheel hub assembly 53 with a clearance. The seat plate 44 can rotate flexibly when inserted into the center hole of the wheel hub assembly 53. The seat plate 44 is clamped and fixed in the wheel hub assembly 53 by the pressing member 52 pressing from the other side.

[0099] Specifically, the hub assembly 53 has a connecting hole 533 on its end face protruding from the outer side of the culture tank wall 11. The connecting hole 533 extends along the axial direction of the hub assembly 53 and is used to fix the stacked member 52.

[0100] Specifically, the stacked member 52 is connected to the hub assembly 53 at their end faces, clamping the seat plate 44 in the middle. The stacked member 52 also has a through hole 521 corresponding to the connecting hole 533 for inserting a fixing screw.

[0101] In this embodiment, the base plate 44 is connected to the tank wall 11 of the culture tank by the clamping of the stacking member 52 and the hub assembly 53, so that the air intake structure 40, which passes through and is fixed from the center of the base plate 44, has more positions in the culture tank. When installing the air intake structure 40, the base plate 44 can rotate at any angle relative to the hub assembly 53, and the posture and position of the aeration section 42 can be changed by rotating the base plate 44, which greatly facilitates the installation of the air intake structure 40 and makes the installation assembly more flexible.

[0102] like Figure 3 As shown, in one embodiment of the present invention, a more flexible intake structure 40 mounting assembly is introduced.

[0103] The inner circumferential surface of the hub assembly 53 is cylindrical. The seat plate 44 is spherical and its radius is equal to the radius of the inner circumferential surface of the hub assembly 53. The stacking member 52 and the support ring 531 rotatably clamp the seat plate 44 in the hub assembly 53.

[0104] In another embodiment, in order to better apply force to the seat plate 44 and distribute the force evenly across the entire cross section of the seat plate 44, the end of the stacked member 52 and the support ring 531 near the inner circumferential surface of the hub assembly 53 is configured as a spherical curved surface.

[0105] In this embodiment, the seat plate 44 is spherical, and the end faces of the stacked member 52 and the support ring 531 that contact the seat plate 44 are hemispherical curved surfaces corresponding to the seat plate 44. This allows the intake structure 40 to rotate flexibly in more directions, and also avoids the problem of stress concentration caused by the force acting on the seat plate 44, thereby improving the service life of the mounting components.

[0106] The present invention also provides a plant tissue culture tank 10 using any of the above-described air intake structures 40 mounting components.

[0107] Specifically, the plant tissue culture vessel 10 has an inverted conical bottom, which reduces the pressure of the nutrient solution on the adventitious roots and promotes their growth. An interface 51 for connecting the air intake structure 40 is provided on the conical side wall. The air intake structure 40 mounting assembly is located at the interface 51.

[0108] In this embodiment, an air intake structure 40 mounting assembly is provided on the plant tissue culture tank 10, which improves the flexibility of the installation and setting of the air intake structure 40, and also helps to give full play to the aeration effect of the air intake structure 40 in the culture tank, which helps to improve the air dissolution rate, and can also promote the circulation of the culture solution in the culture tank, avoid the adventitious roots from tangling and clumping, thereby promoting the faster growth of adventitious roots.

[0109] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An air intake structure for a plant tissue culture vessel, comprising an air intake section (41), an aeration section (42), and a conduit (43) connecting the air intake section (41) and the aeration section (42), characterized in that, The aeration section (42) is provided with a cavity (421) that communicates with the conduit (43), and the cavity wall is provided with a number of densely distributed micropores that communicate with the inside and outside of the cavity (421). The aeration section (42) is formed by stacking and sintering a large number of small particles with a diameter of less than 1 mm. The gap between different small particles forms an airflow channel that extends from the inner surface of the cavity wall to the outer surface of the cavity wall. The intake structure also includes a mounting assembly, which includes: Interface (51) is located on the wall of the plant tissue culture jar; A seat plate (44) is fitted around the outer periphery of the conduit (43) and its outer periphery edge is rotatably connected to the edge of the interface (51) for adjusting the relative position of the aeration section (42) in the plant tissue culture tank. The conduit (43) passes through the center of the seat plate (44) and is connected to the air inlet (41) provided on the other side of the seat plate (44). The conduit (43) is provided with a long straight air inlet section (431), which extends at least a length to both sides of the seat plate (44). The aeration section (42) and the air inlet section (41) are respectively connected to the ends of the air inlet section (431) that extend out of the side of the seat plate (44). An annular stacked component is fitted around the outer periphery of the conduit. The stacked component (52) and the interface (51) on the culture vessel are connected at their end faces and the seat plate (44) is sandwiched between them. The wheel hub assembly (53) has a cylindrical outer circumference. The wheel hub assembly (53) is nested in the interface (51) and its outer circumference is connected to the interface (51). The seat plate (44) of the mounting assembly is fitted with the wheel hub assembly (53) with a gap. The seat plate (44) is fitted into the center hole of the wheel hub assembly (53) and can rotate flexibly. The seat plate (44) is clamped and fixed in the wheel hub assembly (53) by the pressing member (52) pressing from the other side of the seat plate (44).

2. The air inlet structure of a plant tissue culture vessel according to claim 1, characterized in that, One end of the aeration section (42) is connected to the conduit (43) and extends in a straight line away from the conduit (43) to form a long column. The aeration section (42) and the conduit (43) are arranged to coincide with the central axis.

3. The air inlet structure of a plant tissue culture vessel according to claim 1, characterized in that, The conduit (43) is also provided with an air outlet section (432) which is set at an angle to the air inlet section (431). One end of the air outlet section (432) is connected to the air inlet section (431), and the other end is connected to the aeration section (42).

4. The air inlet structure of a plant tissue culture vessel according to claim 3, characterized in that, The duct (43) is also provided with a transition section (433) connecting the air inlet section (431) and the air outlet section (432). The transition section (433) is parallel to the seat plate (44). The air outlet section (432) is inclinedly connected to the transition section (433) and extends away from the seat plate (44).

5. The air inlet structure of a plant tissue culture vessel according to claim 3, characterized in that, The air intake (41) is provided with a quick-release connector (45), which is conical and its bottom surface coincides with the air inlet end face of the duct (43). The conical surface of the quick-release connector (45) faces the seat plate (44) and gradually shrinks to the outer circumference of the duct (43).

6. The air inlet structure of a plant tissue culture vessel according to claim 5, characterized in that, The quick-release connector (45) is provided with a sealing groove (451) that is recessed from the bottom and forms a notch on the bottom surface. The sealing groove (451) is arranged around the outer periphery of the air inlet of the duct (43).

7. A plant tissue culture vessel, characterized in that, The air intake structure described in any one of claims 1-6 is adopted.

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