An adventitious root culture vessel

Abstract

This invention discloses an adventitious root culture tank, comprising a tank body, an exhaust device, and a feeding device. The tank body includes: a tank cover and a discharge port, the discharge port being located at the bottom of the tank body; an exhaust device installed on the tank cover for discharging waste gas from the tank body; a feeding device located above the outer wall of the tank body; an upward-expanding bottom valve located at the bottom of the tank body, fitted into the discharge port; the height from the tank cover to the discharge port is defined as H, the diameter of the tank body is defined as D, and the ratio between the height H and the diameter D is between 1:0.5 and 1:1.2; preferably, the ratio between the height H and the diameter D of the tank body 1 of an adventitious root culture tank is set to 1:1; the feeding device includes a feeding structure, an acid feeding structure, an alkali feeding structure, and an antifoaming liquid feeding structure arranged horizontally in sequence; the middle part of the tank body is cylindrical, and the bottom of the tank gradually tapers from the bottom end of the cylindrical part in the middle of the tank body towards the discharge port to form an inverted conical shape.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of biological culture device, in particular, relates to an adventitious root culture tank. BACKGROUND

[0002] With the continuous improvement of living conditions, people are increasingly paying more attention to the use of medicinal materials with nourishing effect through diet to enhance physical fitness and health care. Due to the huge population, the demand for medicinal plants has increased sharply. And cultivating medicinal plants by traditional methods not only requires a large amount of land, a long growth cycle, but also requires suitable climate. Any unsuitable condition will limit and reduce the yield of medicinal plants.

[0003] In the prior art, a method and a culture device for large-scale cultivation using plant tissues or cells in vitro have been designed. By peeling off the organs of the plant: roots, stems, leaves, etc., and then placing them in a culture medium containing nutrients for cultivation, while providing suitable temperature, light and other environmental conditions for growth, the plant organs are induced to callus, adventitious buds, and adventitious roots. Finally, these callus, adventitious buds, and adventitious roots are used as seeds for cultivating plants, and they are placed in a culture device for cultivation.

[0004] In the prior art, the culture of tissues and cells is carried out in a fermentation reactor. The traditional reactor is made in a ratio of 1:1.5, 1:2. When culturing tissues and cells, especially adventitious roots, due to the height of the culture device, a certain pressure will be generated on the bottom tissues, which is not conducive to tissue growth, and the tissues will also be squeezed during growth. This way of cultivation has limited capacity and is not suitable for large-scale cultivation, and it is also not convenient to maintain. SUMMARY

[0005] The purpose of the present application is to overcome the shortcomings of the prior art and provide a culture tank for tissues and cells, especially a culture tank for adventitious roots.

[0006] To solve the above technical problems, the basic idea of the technical solution of the present application is:

[0007] An adventitious root culture tank, comprising:

[0008] a tank body, a tank bottom, and a discharge port, the discharge port being provided on the tank bottom;

[0009] an exhaust device mounted on the tank cover for exhausting waste gas in the tank body;

[0010] a feeding device provided above the outer sidewall of the tank body;

[0011] The tank body is provided with an upper expansion type tank bottom valve embedded in the discharge port.

[0012] The height between the tank cover and the discharge port is defined as H, and the diameter of the tank body is defined as D. The ratio between the height H and the diameter D of the tank body is between 1:0.5 and 1:1.2.

[0013] Further, the tank body top is provided with a part of the tank cover in the shape of a circular arc, the tank body middle part is provided in the shape of a cylinder, and the tank body further comprises a tank bottom which is gradually tapered in the shape of a circular cone from the bottom end of the tank body middle part cylinder to the direction of the discharge port.

[0014] The tank bottom is provided with at least four groups of air inlet structures, the air inlet structures are connected with air inlet branch pipes through air inlet three-way pipes, the air inlet three-way pipes are connected with air inlet branch pipes, and the air inlet three-way pipes supply air to the air inlet structures.

[0015] Further, the upper expansion type tank bottom valve comprises a valve core, a valve rod,

[0016] The valve core is matched with the shape of the discharge port;

[0017] The valve rod is connected with the valve core and is used for driving the valve core to move along the axial direction of the discharge port;

[0018] The air inlet branch pipe is formed after the air inlet branch pipe extends to the center axis of the tank body for a length and then extends perpendicularly to the air inlet branch pipe for a length, and the lengths of the air inlet branch pipes at both ends are equal.

[0019] Further, the exhaust device comprises:

[0020] An air inlet is used for connecting the exhaust device with the tank body;

[0021] An exhaust port is used for connecting the exhaust device with a waste gas exhaust pipe;

[0022] The exhaust device further comprises cooling water inlets and cooling water outlets arranged on both sides, which are used for condensing and liquefying water vapor in the exhaust device;

[0023] The air inlet branch pipe is connected with the air inlet part of the air inlet structure, and the air inlet branch pipe and the air inlet branch pipe uniformly supply air to the air inlet structure and provide air with the same pressure.

[0024] Further, the feeding device comprises feeding structures, acid supplement structures, alkali supplement structures and defoaming liquid supplement structures which are arranged transversely in sequence.

[0025] The feeding structure comprises a feeding pneumatic valve which is used for controlling the opening and closing or on-off of a feeding pipeline and supplementing the culture solution required in the tank body.

[0026] The acid supplement structure comprises an acid supplement pneumatic valve which is used for controlling the opening and closing or on-off of an acid supplement pipeline and supplementing the acidic solution required in the tank body.

[0027] The alkali supplement structure comprises an alkali supplement pneumatic valve for controlling the opening and closing of the alkali supplement pipeline, and supplementing the alkali solution in the tank;

[0028] The bubble-removing solution supplement structure comprises a bubble-removing solution supplement pneumatic valve for controlling the opening and closing of the bubble-removing solution supplement pipeline, and supplementing the bubble-removing solution in the tank;

[0029] The cutting structure is arranged at the bottom of the tank and comprises at least two groups of cutting parts for cutting the adventitious roots in the culture solution.

[0030] Further, the supplement device comprises a supplement tank, an acid supplement tank, an alkali supplement tank, and a bubble-removing solution supplement tank, which are arranged vertically in sequence.

[0031] When the supplement tank detects that the culture solution in the tank needs to be supplemented, the supplement pneumatic valve is opened, and the culture solution is transported into the tank through the hose.

[0032] The acid supplement tank, the alkali supplement tank, and the bubble-removing solution supplement tank have the same transport mode as the culture solution transport mode of the supplement pneumatic valve.

[0033] The air inlet structure is arranged at the bottom of the tank and comprises at least four groups of air inlets for supplying air into the tank.

[0034] Further, one end of the supplement structure is connected to a supplement air inlet pipe, and the other end is connected to a supplement air outlet pipe. A supplement air inlet valve is arranged on the supplement air inlet pipe, and a supplement air outlet valve is arranged on the supplement air outlet pipe. Before supplementing the culture solution into the tank, the inside of the supplement structure needs to be sterilized through the high-temperature steam pipeline.

[0035] The acid supplement structure, the alkali supplement structure, and the bubble-removing solution supplement structure have the same connection mode with the pipeline.

[0036] The air inlet structure comprises an air inlet rod, which is horizontal and has a certain length. The air inlet rod is in the shape of a long column and is hollow for air inlet. The cavity wall is covered with micropores for air outlet.

[0037] Further, the supplement air inlet pipe of the supplement pneumatic valve is connected to the high-temperature steam air inlet pipe, and the supplement air outlet pipe is connected to the high-temperature steam air outlet pipe.

[0038] The air inlet pipes of the acid supplement pneumatic valve, the alkali supplement pneumatic valve, and the bubble-removing solution supplement pneumatic valve have the same connection mode with the high-temperature steam air inlet pipe.

[0039] The cutting structure comprises a shearing part, which comprises a blade group. The blade group is arranged perpendicularly to the central axis of the cutting structure.

[0040] Further, one end of the exhaust air outlet pipe is connected to the exhaust port of the exhaust device, and the other end is connected to an exhaust filter.

[0041] The blade groups of the cutting structure are arranged on the axis of the cutting part; the blade groups comprise a first blade and a second blade, and the second blade rotates relative to the first blade to form a continuously opening and closing cut.

[0042] Further, the high-temperature steam inlet pipe is connected to a high-temperature steam main pipe, steam in the culture tank enters the high-temperature steam inlet pipe from the high-temperature steam main pipe to each pneumatic valve inlet pipe of the feeding device, and waste gas generated after sterilization is discharged through a high-temperature steam exhaust pipe.

[0043] Compared with the prior art, the technical scheme has the following beneficial effects.

[0044] 1. According to the growth environment required by adventitious roots, one end of the tank body of the culture tank is a cylinder, and the other end is a reverse cone, the height of the culture tank from the tank cover to the discharge port is in a ratio of 1:1 with the diameter of the tank body, compared with the height-diameter ratio of 1:1.5 or 1:2 of the traditional adventitious root culture tank, the adventitious root culture tank reduces the pressure on the bottom adventitious roots due to the height, and the height-diameter ratio of 1:1 of the tank body makes the internal space of the culture tank more uniform and spacious, so that the adventitious roots have enough space to expand and develop during the growth process. In addition, compared with the tank body with a larger height, the height-diameter ratio of 1:1 reduces the mutual extrusion between the adventitious roots. The height-diameter ratio of 1:1 of the adventitious root culture tank of the present application is more conducive to the growth of adventitious roots.

[0045] 2. The exhaust device of the adventitious root culture tank is arranged at the tank cover of the culture tank, and two small pipes are arranged on the side of the exhaust device for cooling water, and the cooling water pipeline provides cooling when the temperature in the culture tank is too high. The exhaust device can effectively exhaust the waste steam in the tank body, control the gas pressure in the culture tank, and appropriately adjust the gas pressure in the tank body.

[0046] 3. The feeding device of the adventitious root culture tank is arranged above the outer sidewall of the tank body for supplementing acid, alkali, culture solution and defoaming solution into the tank body. The feeding device reduces the need for manual operation and reduces the possibility of unnecessary pollutants entering the tank body. Through accurate control and metering, it ensures that the materials are supplemented according to the predetermined ratio, and improves the operation convenience.

[0047] The specific embodiments of the present application will be further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0048] The accompanying drawings, which are part of the present application, serve to provide a further understanding of the present application, and the schematic embodiments of the present application and their descriptions serve to explain the present application, but do not constitute an improper limitation on the present application. Obviously, the accompanying drawings in the following description are only some embodiments, and other drawings can be obtained by those skilled in the art without creating creative labor. In the drawings:

[0049] Figure 1 Figure 1 is a schematic diagram of the tank body structure of the culture tank of the present application;

[0050] Figure 2 Figure 5 is a schematic diagram of the exhaust device;

[0051] Figure 3 Figure 6 is a schematic diagram of the feeding device;

[0052] Figure 4 Figure 7 is a schematic diagram of the up-and-out tank bottom valve on the bottom of the tank body;

[0053] Figure 5 Figure 8 is a schematic diagram of the discharge port structure;

[0054] Figure 6 Figure 9 is a schematic diagram of the side of the culture tank;

[0055] Figure 7 Figure 10 is a schematic diagram of the connection of the air inlet structure of the tank bottom to the air inlet pipeline;

[0056] Figure 8 Figure 11 is a schematic diagram of the overall structure of the culture tank of the present application;

[0057] Figure 9 Figure 12 is a schematic diagram of the air inlet structure and the cutting structure located on the bottom of the tank body of the present application;

[0058] Figure 10 Figure 13 is a schematic diagram of the cutting structure located on the side wall of the tank bottom of the present application;

[0059] Figure 11 Figure 14 is a schematic diagram of the air inlet structure located on the side wall of the tank bottom of the present application;

[0060] Figure 12 Figure 15 is a schematic diagram of the air inlet structure of a root culture tank of the present application;

[0061] Figure 13 Figure 16 is a schematic diagram of the cutting structure of a root culture tank of the present application;

[0062] Figure 14 Figure 17 is a schematic diagram of the cutting part of the cutting structure of the present application.

[0063] Explanation of reference numerals in the drawings: 1, tank body; 2, tank cover; 3, discharge port; 30, upper expansion tank bottom valve; 31, valve core; 32, valve stem; 321, first through pipe; 322, isolation sleeve; 323, second through pipe; 33, discharge pipe; 34, through hole; 35, connecting wall; 36, support part; 37, connecting part; 38, driving assembly; 39, support wall; 4, exhaust device; 40, air inlet; 41, cooling water inlet pipe; 42, cooling water outlet pipe; 43, exhaust port; 5, tail gas filter; 6, replenishment device; 7, heat preservation sandwich; 71, steam air inlet valve; 72, steam air outlet valve; 73, feed pipe; 10, high-temperature steam main pipe; 102, high-temperature steam air inlet pipe; 103, exhaust gas exhaust pipe; 104, high-temperature steam exhaust pipe; 11, tank bottom; 13, replenishment structure; 14, acid replenishment structure; 15, alkali replenishment structure; 16, defoaming liquid replenishment structure; 113, replenishment pneumatic valve; 114, acid replenishment pneumatic valve; 115, alkali replenishment pneumatic valve; 116, defoaming liquid replenishment pneumatic valve; 131, replenishment air inlet pipe; 132, replenishment air outlet pipe; 141, acid replenishment air inlet pipe; 142, acid replenishment air outlet pipe; 151, alkali replenishment air inlet pipe; 152, alkali replenishment air outlet pipe; 161, defoaming liquid replenishment air inlet pipe; 162, defoaming liquid replenishment air outlet pipe; 133, replenishment air inlet valve; 134, replenishment exhaust valve; 143, acid replenishment air inlet valve; 144, acid replenishment exhaust valve; 153, alkali replenishment air inlet valve; 154, alkali replenishment exhaust valve; 163, defoaming liquid replenishment air inlet valve; 164, defoaming liquid replenishment exhaust valve; 130, replenishment tank; 140, acid replenishment tank; 150, alkali replenishment tank; 160, defoaming liquid replenishment tank; 105, replenishment hose; 106, acid replenishment hose; 107, alkali replenishment hose; 108, defoaming liquid replenishment hose; 12, air inlet structure; A1106, air inlet branch pipe; A1107, air inlet sub-pipe; B8, air inlet tee pipe; 20, cutting structure; 201, isolation sleeve; 202, shearing part; 203, transmission part; 204, power part; 21, air guide support; 211, first straight section; 212, second straight section; 213, third straight section; 22, air inlet part; 23, seat plate; 24, air vent rod; 51, interface; 325, blade set; 326, first blade; 324, second blade.

[0064] It should be noted that these drawings and written descriptions are not intended to limit the scope of the concept of the present application in any way, but to illustrate the concept of the present application to those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0065] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments will be described clearly and completely below with reference to the drawings in the embodiments of the present application. The following embodiments are used to illustrate the present application, but are not used to limit the scope of the present application.

[0066] In the description of the present application, it should be noted that the terms "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0067] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "contacting", "communicating" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0068] As shown in Figure 1 The present embodiment discloses an adventitious root culture tank, which comprises a tank body 1, a tank cover 2, and a discharge port 3. The tank cover 2 is partially provided in a semi-elliptical shape on the top of the tank body 1, the middle part of the tank body 1 is provided in a cylindrical shape, and the tank bottom 11 is provided in a reverse conical shape. The tank bottom 11 gradually shrinks from the bottom end of the cylindrical middle part of the tank body 1 to the direction of the discharge port 3 to form a reverse conical structure, and the shrinkage angle is greater than 30° and less than 180°. The inside of the tank body 1 is hollow.

[0069] As shown in Figure 1 The height between the tank cover 2 and the discharge port 3 of the tank body 1 of the culture tank is defined as H, and the diameter of the culture tank is defined as D. The ratio between the height H and the diameter D is between 1:0.5 and 1:1.2. When the adventitious root culture is carried out, when the ratio between the height H and the diameter D of the tank body 1 exceeds 1:0.5, the culture solution accumulates at the tank bottom 11, which causes the water pressure at the tank bottom 11 to be too high and affects the growth of the adventitious roots. When the ratio between the height H and the diameter D of the tank body 1 is less than 1:1.2, the growth rate of the adventitious roots slows down, and the adventitious roots in the tank body 1 are pressed against each other, which is not conducive to the growth of the adventitious roots. Preferably, the ratio between the height H and the diameter D of the tank body 1 of the adventitious root culture tank is set to 1:1, which solves the technical problem of the liquid pressure on the adventitious roots at the tank bottom 11. For the adventitious roots, the growth environment is crucial. Through experimental comparison, the growth amount of the adventitious roots in the culture tank with the ratio of height H to diameter D of 1:1 is 70-90 g / L, and the growth amount of the adventitious roots in the culture tank with the ratio of height H to diameter D of 1:1.2 is 40-50 g / L. Obviously, the ratio of height to diameter of 1:1 is more conducive to the growth of the adventitious roots.

[0070] AsFigure 1 、 2 As shown in FIG. 1, a root culture tank includes an exhaust device 4, which is provided in a hollow cylindrical shape and made of polytetrafluoroethylene material with corrosion resistance and high temperature resistance. The exhaust device 4 includes an air inlet 40 provided at the bottom end and connected to the tank body 1, and an exhaust port 43 provided at the top end of the exhaust device 4 and connected to the exhaust pipe 103. The exhaust device 4 further includes a cooling water inlet pipe 41 and a cooling water outlet pipe 42 on both sides.

[0071] In this embodiment, the main function of the exhaust device 4 is to exhaust the waste steam in the tank body 1. The water vapor in the tank body 1 needs to be controlled within a certain range. Too little water vapor in the tank body 1 will cause the concentration of the culture solution in the tank body 1 to increase, affecting the growth of the adventitious roots. When part of the water vapor in the tank body 1 is exhausted into the exhaust device 4, the water vapor is condensed and liquefied under the action of cooling water and flows back into the tank body 1.

[0072] As shown in FIG. 2, the culture tank needs to provide a certain amount of air for the growth of the adventitious root plant tissue in the culture tank and the respiration of the adventitious roots in the tank body 1. Therefore, a certain amount of air is usually dissolved in the culture solution. During the circulation of the culture solution, gas gradually separates from the culture solution. The carbon dioxide and other waste gas generated by the respiration of the adventitious roots are exhausted into the exhaust pipe 103 through the exhaust device 4. The other end of the exhaust pipe 103 is also provided with an exhaust filter 5. Figure 1 The exhaust filter 5 is connected to the exhaust pipe 103 and includes a multi-element filter element with a stainless steel filter screen. The exhaust filter 5 is used to filter harmful gases in the exhaust pipe 103 and has an anti-suction function to prevent bacteria or impurities in the external air from entering the culture tank. In addition, the filtered gas is exhausted into the air.

[0073] As shown in FIG. 3, the root culture tank also includes a feeding device 6. The feeding device 6 is fixed to the upper side of the outer wall of the tank body 1 of the culture tank. The feeding device 6 includes feeding structure 13, acid supplementing structure 14, alkali supplementing structure 15, and defoaming liquid supplementing structure 16 arranged transversely in sequence. The feeding device 6 has feeding inlet pipe 131, acid supplementing inlet pipe 141, alkali supplementing inlet pipe 151, and defoaming liquid supplementing inlet pipe 161. The four groups of inlet pipes are connected to the high-temperature steam inlet pipe 102. The feeding device 6 also includes feeding outlet pipe 132, acid supplementing outlet pipe 142, alkali supplementing outlet pipe 152, and defoaming liquid supplementing outlet pipe 162. The four groups of outlet pipes are connected to the high-temperature steam exhaust pipe 104. The four groups of structures are sterilized by the high-temperature steam pipe.

[0074] Figure 3 As shown in FIG. 4, the root culture tank also includes a feeding device 6. The feeding device 6 is fixed to the upper side of the outer wall of the tank body 1 of the culture tank. The feeding device 6 includes feeding structure 13, acid supplementing structure 14, alkali supplementing structure 15, and defoaming liquid supplementing structure 16 arranged transversely in sequence. The feeding device 6 has feeding inlet pipe 131, acid supplementing inlet pipe 141, alkali supplementing inlet pipe 151, and defoaming liquid supplementing inlet pipe 161. The four groups of inlet pipes are connected to the high-temperature steam inlet pipe 102. The feeding device 6 also includes feeding outlet pipe 132, acid supplementing outlet pipe 142, alkali supplementing outlet pipe 152, and defoaming liquid supplementing outlet pipe 162. The four groups of outlet pipes are connected to the high-temperature steam exhaust pipe 104. The four groups of structures are sterilized by the high-temperature steam pipe.

[0075] As shown in FIG. 5, the root culture tank also includes a feeding device 6. The feeding device 6 is fixed to the upper side of the outer wall of the tank body 1 of the culture tank. The feeding device 6 includes feeding structure 13, acid supplementing structure 14, alkali supplementing structure 15, and defoaming liquid supplementing structure 16 arranged transversely in sequence. The feeding device 6 has feeding inlet pipe 131, acid supplementing inlet pipe 141, alkali supplementing inlet pipe 151, and defoaming liquid supplementing inlet pipe 161. The four groups of inlet pipes are connected to the high-temperature steam inlet pipe 102. The feeding device 6 also includes feeding outlet pipe 132, acid supplementing outlet pipe 142, alkali supplementing outlet pipe 152, and defoaming liquid supplementing outlet pipe 162. The four groups of outlet pipes are connected to the high-temperature steam exhaust pipe 104. The four groups of structures are sterilized by the high-temperature steam pipe. Figure 6 ​As shown, an insulation layer 7 is provided on the outer periphery of the tank body 1. A feeding device 6 is provided on the upper side wall of the non-insulation layer 7 of the tank body 1. The feeding device 6 has a feeding structure 13, an acid feeding structure 14, an alkali feeding structure 15, and an antifoaming liquid feeding structure 16, which are arranged in a ring around the upper side wall of the non-insulation layer 7 of the tank body 1. It is connected to the tank body 1 through a pipeline. Before feeding, the inside of the feeding device 6 needs to be sterilized by steam. The steam enters from the top and exits from the bottom, as shown by the arrow in the figure, from the steam inlet valve 71 to the steam outlet valve 72 for sterilization. During sterilization, the feed pipe 73 is closed. After sterilization, the steam inlet valve 71 and the steam outlet valve 72 are closed, and the feeding valve 73 is opened.

[0076] In this embodiment, before replenishing the solution into the tank 1 through the feeding device 6, the feeding device 6 needs to be sterilized with high-temperature steam. The setting of the high-temperature steam inlet pipe 102 and the high-temperature steam exhaust pipe 104 achieves the effect of sterilizing the inside of the feeding device 6. The high-temperature steam required for sterilization enters the feeding device 6 through the high-temperature steam inlet pipe 102, and the sterilized exhaust gas is discharged through the high-temperature steam exhaust pipe 104. This high-temperature sterilization method realizes the aseptic culture technology of adventitious roots.

[0077] The high-temperature steam inlet pipe 102 and the high-temperature steam outlet pipe 104 are made of ceramic titanium filter membrane material. The steam in the high-temperature steam pipe passes through the ceramic titanium filter membrane for filtration. The ceramic titanium filter membrane is used to filter microorganisms and impurities in the high-temperature steam. The ceramic titanium filter membrane adopts a multi-stage filtration structure to improve the filtration effect and lifespan, making the filtered high-temperature steam cleaner and more thoroughly disinfected.

[0078] like Figure 1 As shown, an adventitious root culture tank includes a feeding device 6 that further comprises a feeding tank 130, an acid feeding tank 140, an alkali feeding tank 150, and an antifoaming solution feeding tank 160 for detecting the remaining solution or stored solution in the tank 1. The four tanks are connected to their corresponding structures via hoses. Based on the remaining solution in the four tanks or the need to replenish any missing culture medium, acidic solution, alkaline solution, or antifoaming solution in the tank 1, the feeding device 6 opens the corresponding pneumatic valve and replenishes the corresponding solution to the tank 1, or adjusts the acidity or alkalinity of the culture medium or replenishes the antifoaming solution. The feeding process can be manually controlled or automatically replenished based on the pH value detected by the acid feeding tank 140 and the alkali feeding tank 150, or the foam generation detected by the antifoaming solution feeding tank 160 in the tank 1. The required culture medium in the tank 1 is replenished in stages.

[0079] like Figure 3As shown, the feeding structure 13 in the feeding device 6 of an adventitious root culture tank includes a feeding pneumatic valve 113, a feeding inlet pipe 131, a feeding outlet pipe 132, a feeding inlet valve 133, a feeding exhaust valve 134, a feeding hose 105, and a feeding box 130. The feeding pneumatic valve 113 is installed on the feeding hose 105 and is used to control the opening and closing of the feeding pipeline. One end of the feeding hose 105 is connected to the feeding structure 13, and the other end is connected to the feeding box 130. One end of the feeding structure 13 is connected to the feeding inlet pipe 131, and the other end is connected to the feeding outlet pipe 132. The feeding inlet pipe 131 is equipped with the feeding inlet valve 133, and the feeding outlet pipe 132 is equipped with the feeding exhaust valve 134. The feeding inlet pipe 131 is connected to the high-temperature steam inlet pipe 102, and the feeding outlet pipe 132 is connected to the high-temperature steam exhaust pipe 104. The high-temperature steam inlet pipe 102 and the high-temperature steam exhaust pipe 104 are respectively fixed to the side wall of the tank body 1.

[0080] An acid-supplementing structure 14 in a feeding device 6 for an adventitious root culture tank includes an acid-supplementing pneumatic valve 114, an acid-supplementing air inlet pipe 141, an acid-supplementing air outlet pipe 142, an acid-supplementing air inlet valve 143, an acid-supplementing air outlet valve 144, an acid-supplementing hose 106, and an acid-supplementing box 140.

[0081] An alkali replenishment structure 15 in a feeding device 6 for an adventitious root culture tank includes an alkali replenishment pneumatic valve 115, an alkali replenishment air inlet pipe 151, an alkali replenishment air outlet pipe 152, an alkali replenishment air inlet valve 153, an alkali replenishment air outlet valve 154, an alkali replenishment hose 107, and an alkali replenishment box 150.

[0082] The defoaming liquid replenishment structure 16 in the feeding device 6 of an adventitious root culture tank includes a defoaming liquid replenishment pneumatic valve 116, a defoaming liquid replenishment air inlet pipe 161, a defoaming liquid replenishment air outlet pipe 162, a defoaming liquid replenishment air inlet valve 163, a defoaming liquid replenishment exhaust valve 164, a defoaming liquid replenishment hose 108, and a defoaming liquid replenishment tank 160.

[0083] The connection method between the acid replenishing structure 14, the alkali replenishing structure 15, and the defoaming liquid replenishing structure 16 and the pipeline is the same as the connection method between the material replenishing structure 13 and the pipeline. This connection provides a more convenient way to inject nutrients and realizes the technology of systematically controlling the injection of nutrients into the tank 1.

[0084] The feeding structure 13 is used to replenish the culture medium required in the tank 1. When it is necessary to replenish the culture medium in the tank, high-temperature steam is first introduced into the high-temperature steam inlet pipe 102, and then the feeding inlet valve 133 is opened to introduce high-temperature steam into the feeding inlet pipe 131. The high-temperature steam is then introduced into the feeding structure 13 for high-temperature steam sterilization. At the same time, the feeding exhaust valve 134 on the feeding exhaust pipe 132 is opened to discharge the sterilized waste steam into the high-temperature steam exhaust pipe 104. During feeding, the tank 1 is kept under positive pressure to prevent external bacteria from entering. After feeding, the culture is carried out at room temperature of 20-25℃.

[0085] The acid-replenishing structure 14 is used to replenish the acidic solution required in the tank 1. When the pH of the culture medium in the tank 1 is less than 7, the solution is acidic. If the acidity of the culture medium is too high, it will affect the growth of adventitious roots, resulting in the inhibition of adventitious root reproduction, reduction in the number of adventitious roots, or cessation of growth. Therefore, it is necessary to replenish the alkali according to the pH value in the tank 1.

[0086] Alkali replenishment structure 15 is used to replenish the alkaline solution required in tank 1. When the pH of the culture medium in tank 1 is >7, the solution is alkaline. If the alkalinity of the culture medium is too high, it will affect the growth of adventitious roots. Therefore, acid replenishment is required according to the pH value in tank 1. The pH value of the culture medium in tank 1 should be controlled within an appropriate range.

[0087] The defoaming liquid replenishment structure 16 is designed to address the issue that the liquid inside the tank 1 causes the adventitious roots to tumble up and down, generating bubbles. These bubbles cause changes in the concentration of the culture medium. Excessive bubbles can affect the growth of the adventitious roots. A preset value X is set for the standard height of the foam inside the tank 1. When the generated foam exceeds the preset value X, the defoaming liquid replenishment pneumatic valve 116 opens and replenishes the tank 1 with defoaming liquid as needed to eliminate or reduce the foam on the surface of the culture medium.

[0088] like Figure 1 , 3 As shown, a feeding hose 105 of an adventitious root culture tank is connected at one end to a feeding structure 13 and at the other end to a feeding box 130. An acid feeding hose 106 is connected at one end to an acid feeding structure 14 and at the other end to an acid feeding box 140. An alkali feeding hose 107 is connected at one end to an alkali feeding structure 15 and at the other end to an alkali feeding box 150. An antifoaming solution feeding hose 108 is connected at one end to an antifoaming solution feeding structure 16 and at the other end to an antifoaming solution feeding box 160.

[0089] like Figure 1 As shown, steam from an adventitious root culture tank enters the high-temperature steam inlet pipe 102 from the high-temperature steam main pipe 10 above the tank body 1, and then enters the tank body 1 through the pipeline in sequence. The waste steam in the tank body 1 is discharged to the waste gas exhaust pipe 103 through the exhaust device 4. The steam in the waste gas exhaust pipe 103 is filtered by the tail gas filter 5 and then discharged into the air.

[0090] like Figure 1 , 4 As shown, the bottom of the tank body 1 is also equipped with an upward-expanding bottom valve 30. The bottom end of the upward-expanding bottom valve 30 of the tank body 1 is equipped with a discharge pipe 33. The discharge pipe 33 is equipped with an "L"-shaped bend. One end is connected to the bottom discharge port 3 of the tank body 1, and the other end is connected to the pipeline for replanting or discharge.

[0091] like Figure 4As shown, the top-mounted tank bottom valve 30 includes a valve core 31 and a valve stem 32. The valve core 31 is adapted to the shape of the discharge port 3 and is fitted into the discharge port 3 from the inside of the tank bottom 11. The top surface of the valve core 31 and the inner wall of the tank bottom 11 on the outer periphery of the discharge port 3 have a smooth transition. The valve stem 32 extends along the axial direction of the discharge port 3 and passes through the pipe wall of the discharge pipe 33 from the corner of the discharge pipe 33, extending to the outside of the discharge pipe 33. The valve core 31 is connected to the valve stem 32. The valve core 31 is used to control the closing and opening of the discharge port 3, and the valve stem 32 is used to drive the valve core 31 to move along the axial direction of the discharge port 3.

[0092] like Figure 4 , 5 As shown, the discharge port 3 includes: a through hole 34, which is located at the bottom of the tank 11 and is adapted to the shape of the valve core 31; and a connecting wall 35, which extends downward from the bottom of the tank 11 and is coaxial with the through hole 34, for connecting the discharge pipe 33.

[0093] Specifically, the inner diameter of the connecting wall 35 is larger than the diameter of the through hole 34. The end of the discharge pipe 33 near the tank body 1 is provided with: a support part 36, which is inserted into the connecting wall 35 and abuts against the bottom of the tank 11; and a connecting part 37, which is provided at the end of the support part 36 away from the bottom of the tank 11, extends outward along the radial direction of the support part 36, and abuts against the lower end of the connecting wall 35.

[0094] In this embodiment, the thickness of the end of the connecting wall 35 away from the bottom of the tank 11 is greater than the thickness of the end closer to the bottom of the tank 11. Correspondingly, the outward extension length of the connecting part 37 is adapted to the thickness of the end of the connecting wall 35 away from the bottom of the tank 11. The larger the contact area between the connecting part 37 and the connecting wall 35, the stronger the connection.

[0095] like Figure 4 , 5 As shown, the inner diameter of the discharge pipe 33 is smaller than the diameter of the through hole 34. The end of the discharge pipe 33 near the tank 1 is provided with a support wall 39 whose diameter gradually increases along the direction of approaching the tank 1. The inner wall of the through hole 34 and the inner wall of the discharge pipe 33 are connected by the support wall 39. The bottom of the valve core 31 is in contact with the support wall 39.

[0096] like Figure 4 As shown, a drive assembly 38 is also provided below the tank body 1, which is connected to the end of the valve stem 32 away from the valve core 31 and is used to drive the valve stem 32 to move axially along the discharge port 3.

[0097] like Figure 4 As shown, a first through pipe 321 is provided extending from the notch edge at the corner of the discharge pipe 33 away from the discharge port 3. The inner diameter of the first through pipe 321 is larger than the diameter of the valve stem 32. The valve stem 32 is located inside the first through pipe 321 and includes an isolation sleeve 322, which is located between the first through pipe 321 and the valve stem 32. The isolation sleeve 322 is in contact with the first through pipe 321 and the valve stem 32.

[0098] At one end of the drive assembly 38 near the discharge port 3, a second passage pipe 323 is provided extending from the middle of the drive assembly 38 away from the discharge port 3. The inner diameter of the second passage pipe 323 is adapted to the diameter of the valve stem 32. The end of the first passage pipe 321 away from the discharge port 3 is abutted and fixed to the end of the second passage pipe 323 near the discharge port 3.

[0099] The drive assembly 38 is located outside the discharge pipe 33. The discharge pipe 33 needs to be completely sealed. If the drive assembly is located inside the discharge pipe 33, the wiring of the drive assembly 38 needs to pass through the tank 1 to connect to the outside. At the same time, a large amount of adventitious roots and adventitious root culture solution will frequently flow through the discharge pipe 33. The drive assembly 38 may also malfunction after being immersed in the liquid for a long time. If the drive assembly 38 is located inside the discharge pipe 33, the transfer of adventitious roots and adventitious root culture solution will not be smooth. The adventitious roots will also get stuck on the drive assembly 38, which will further reduce the passability of the discharge pipe 33 and may eventually lead to blockage of the discharge pipe 33.

[0100] In this embodiment, by placing the drive component 38 outside the discharge pipe 33 and connecting the drive component 38 and the discharge pipe 33 into a whole through a through pipe, the drive component 38 can be placed outside the discharge pipe 33 while still being able to control the opening or closing state of the discharge port 3.

[0101] The isolation sleeve 322 is designed to ensure a tight seal between the valve stem 32 and the discharge pipe 33. When the discharge port 3 is open, the adventitious roots and culture medium inside the tank flow into the discharge pipe 33. The first connecting pipe 321 is connected to the discharge pipe 33. When the liquid flows through, it can easily flow into the drive assembly 38 through the gap, causing damage to the device. On the other hand, the gap at the junction of the first connecting pipe 321 and the discharge pipe 33 can cause the adventitious roots to get caught in the gap, resulting in damage to the adventitious roots. It can also cause the adventitious roots to accumulate at the junction of the first connecting pipe 321 and the discharge pipe 33, affecting the normal operation of the valve stem 32. Therefore, the isolation sleeve 322 is provided to avoid the above situations.

[0102] In this embodiment, the opening and closing of the discharge port 3 is controlled by manually or automatically controlling the upward-expanding bottom valve 30. When the valve core 31 moves away from the interior of the tank 1, the discharge port 3 closes; when the valve core 31 moves towards the interior of the tank 1, the discharge port 3 opens. Preferably, after the upward-expanding bottom valve 30 is pushed upward, the annular interception area of ​​the gap between the outer circumferential surface of the valve core 31 and the side wall of the tank bottom 11 is equal to the cross-sectional area of ​​the discharge pipe 33. When it is extended to this extent, the effect of adventitious roots or culture medium in the culture tank entering the discharge pipe 33 is optimal.

[0103] When adventitious roots or culture medium need to flow from outlet 3 into outlet pipe 33, the upward-expanding bottom valve 30 reduces the accumulation of adventitious roots in outlet pipe 33 and prevents contamination due to the liquid pressure. In addition, the isolation sleeve 322 of drive assembly 38 can prevent adventitious roots or culture medium from flowing into first through pipe 321 or second through pipe 323.

[0104] In this embodiment, the bottom 11 of the tank is provided with at least four sets of evenly distributed air intake structures 12, which are equally spaced. The air intake structures 12 supply sterile air to the tank body 1. During air supply, the flow of gas provides better stirring for the adventitious roots and culture medium in the tank body 1, and can provide sufficient sterile air for the adventitious roots to respire. A good supply of sterile air can promote the respiration and metabolism of adventitious roots and the growth of adventitious root systems.

[0105] Four sets of air intake structures 12 are connected in pairs to one air intake branch pipe A1106. Taking one air intake branch pipe A1106 as an example, the extension distance of the air intake branch pipe A1106 is less than the maximum radius of the culture tank. After extending a certain distance, the air intake branch pipe A1106 bends towards the front of the main support frame and extends through the main support frame to the same plane as the central axis of the culture tank. This plane is parallel to the front of the main support frame, so that the two air intake branch pipes A1106 are symmetrically located below the culture tank and close to the center of the bottom of the culture device. After extending a certain distance, the air intake branch pipe A1106 extends vertically upwards for a certain distance and then extends two air intake branch pipes A1107 to both sides through the air intake tee pipe B8. The two air intake branch pipes A1107 The distance between the air intake structure 12 and the central axis of the culture tank is greater than the distance between the central axis of the culture tank and the air intake structure 12. The air intake structure 12 bends upward at an angle and extends to connect with the two air intake structures 12 on the same side of the bottom of the culture tank. Another air intake branch pipe A1106 is symmetrically distributed with it. The two air intake branch pipes A1107 on the symmetrical side are connected to the two air intake structures 12 on the same side. The angle between the upward-bending pipe and the pipe extending through the tee pipe is less than 90°. The pipe is connected to the four air intake structures 12 at the bottom of the culture tank through the air intake tee pipe B8. Air is evenly supplied to the culture tank through the air intake branch pipe A1107 extending from the air intake tee pipe B8, which greatly improves the contact surface between air and culture medium. The pipes of the extended frame are hidden under the tank body, making full use of space without affecting the layout of other pipes. The air intake structure 12 supplies air evenly to the inside of the tank body 1. The waste gas generated during the cultivation of adventitious roots is discharged through the exhaust device 4.

[0106] The intake manifold (A1106) extends a certain length in the direction of the central axis of the tank (1) and then extends a certain length in the direction perpendicular to the intake manifold (A1106) to form an intake branch pipe (A1107). The intake branch pipes (A1107) at both ends of the intake manifold (A1106) are of equal length.

[0107] The intake branch pipe (A1107) is connected to the intake section (22) of the intake structure (12). The intake branch pipe (A1107) and the intake manifold (A1106) supply gas to the intake structure (12) evenly, providing gas at the same pressure.

[0108] like Figure 8 , 9 As shown, this embodiment discloses an adventitious root culture vessel, including a vessel body 1, a vessel lid 4, and a discharge port 3. The top of the vessel body 1 has a portion of the vessel lid 4 in a semi-spherical shape, the middle of the vessel body 1 has a cylindrical shape, and the bottom of the vessel body 1 has an inverted conical shape. The inverted conical portion gradually tapers from the side wall of the vessel body 1 towards the bottom discharge port 3, and the vessel body 1 is hollow. The inverted conical bottom of the vessel body 1 has an air inlet structure 12 for supplying sterile air into the vessel body 1 and a cutting structure 20 for shearing the adventitious roots inside the vessel body 1. The bottom 11 of the vessel body has mounting holes for the air inlet structure 12 and mounting holes for the spacer 201 of the cutting structure 20 to pass through; the two types of mounting holes have different sizes.

[0109] On the tank body 1, the line connecting the installation positions of the cutting structure 20 and the two adjacent air intake structures 12 approximately forms an isosceles triangle, and the installation position of the cutting structure 20 is higher than that of the air intake structure 12 in the vertical direction.

[0110] The mounting positions of the air intake structure 12 mounting hole and the cutting structure 20 mounting hole at the bottom of the tank 11 approximately form an isosceles triangle. The positions of the two sets of cutting structure 20 mounting holes are on the same horizontal plane relative to the central axis of the tank body 1, and the mounting position of the cutting structure 20 is higher than the mounting position of the air intake structure 12.

[0111] like Figure 10 As shown, an adventitious root culture vessel includes a cutting structure 20 with a spacer 201. A circular mounting hole is formed in the bottom 11 of the culture vessel. The outer circumferential surface of the spacer 201 is cylindrical and has the same diameter as the mounting hole. The spacer 201 is detachably installed in the mounting hole on the bottom 11. One side of the spacer 201 is connected to a shearing part 202, and the other side is connected to a power unit 204, separating the shearing part 202 and the power unit 204 inside and outside the culture vessel. The shearing part 202 is installed on the side of the spacer 201 facing inwards from the culture vessel 1, and the power unit 204 is installed on the side of the spacer 201 facing outwards from the culture vessel. The power unit 204 drives the shearing part 202 to cut the adventitious roots. The cross-section of the shearing part 202 is smaller than the cross-section of the spacer 201, allowing the cutting structure 20 to be easily and quickly installed on the adventitious root culture vessel.

[0112] like Figures 13-14As shown, the shearing section 202 of the cutting structure 20 of an adventitious root culture vessel includes at least two sets of blade groups 325 connected in series on a rotating shaft. The blade groups 325 are evenly distributed along the axial direction of the rotating shaft, forming a multi-layer structure. Each blade group 322 is composed of a first blade 326 and a second blade 324 fitted together in pairs.

[0113] Specifically, all the first blades 326 in the blade assembly 325 are parallel to each other and fixed to the rotating shaft. The second blade 324 rotates relative to the first blades 326 to form a continuously opening and closing slit. When the second blade 324 is driven to rotate relative to the first blade 326, adjacent blade assemblies 325 simultaneously cut the adventitious roots entering the shearing section 202. The gap between each blade assembly 325 is 1.5 cm.

[0114] like Figure 13 As shown, the cutting structure 20 also includes a transmission section 203 between the shearing section 202 and the power section 204. Specifically, the transmission section 203 is cylindrical and is located inside the spacer 201 near one end of the shearing section 202. The outer surface of the inner cylinder of the transmission section 203 and the inner surface of the outer periphery of the spacer 201 can be slidably connected or rotatably connected and connected together by a bearing.

[0115] like Figures 9-10 As shown, the bottom 11 of an adventitious root culture tank of the present invention is provided with at least two sets of cutting structures 20. The two sets of cutting structures 20 are symmetrically arranged on both sides of the bottom 11 of the tank body 1 with the discharge port 3 at the bottom of the tank body 1 as the center, and the distance between the installation position of the two sets of cutting structures 20 and the discharge port 3 is equal. One end of the cutting structure 20 with the power unit 204 extends outward to the outside of the tank body 1, and one end with the shearing part 202 extends inward toward the central axis of the tank body 1, and is nearly perpendicular to the position formed with the bottom 11 of the tank. The cutting structure 20 is provided at the bottom 11 of the tank to cut the adventitious roots that have grown to a certain number or a certain volume to a length suitable for secondary culture of adventitious roots. Preferably, the adventitious roots are cut into small segments of 5-15 mm.

[0116] like Figures 11-12 As shown, this embodiment discloses an installation assembly for an air intake structure 12 of an adventitious root culture tank. The air intake structure 12 is detachably connected to the bottom 11 of the adventitious root culture tank. The installation assembly for the air intake structure 12 includes a base plate 23 and an interface 51. The interface 51 is formed on the bottom 11 of the adventitious root culture tank. The air intake structure 12 is fixedly connected to the base plate 23. By rotating the base plate 23 relative to the interface 51 of the culture tank, the relative position of the ventilator 24 of the air intake structure 12 within the culture tank can be adjusted. This allows the air intake structure 12 to have more relative positions and orientations relative to the culture tank, improving the flexibility of installation and setup of the air intake structure 12, and also facilitating the full utilization of the ventilation function of the air intake structure 12 within the culture tank.

[0117] The air intake structure 12 includes an air intake section 22, an air vent 24, and an air guide bracket 21 connecting the air intake section 22 and the air vent 24. It also includes a seat plate 23 that can be embedded in the interface 51. The seat plate 23 is sleeved on the outer periphery of the air guide bracket 21 and its edge is connected to the edge of the interface 51, separating the air vent 24 from the air intake section 22 inside and outside the adventitious root culture tank.

[0118] like Figure 12 As shown, the air intake structure 12 includes an air guide bracket 21, which is a bent pipe comprising a first straight section 211, a second straight section 212, and a third straight section 213. The first straight section 211 is perpendicular to the second straight section 212, and the second straight section 212 and the third straight section 213 are set at an obtuse angle, with a smooth transition between the first straight section 211 and the second straight section 212, and between the second straight section 212 and the third straight section 213. One end of the first straight section 211 is provided with an air intake part 22 for conveying sterile air. A venting rod 24 is fitted around the outer periphery of the third straight section 213. Gas enters the air guide bracket 21 from the air intake part 22 of the first straight section 211 of the air intake structure 12, and sequentially passes through the second straight section 212 and the third straight section 213 to the venting rod 24. Sterile air is then conveyed into the tank 1 through the micropores on the surface of the venting rod 24. The air intake effect is optimal when the venting rod 24 is in a horizontal position.

[0119] The ventilation rod 24 is hollow inside, and its surface is densely covered with numerous micropores made of a material that can be sintered to a pore size of 1-20 μm. The micropores are spaced apart and extend from the inner to the outer surface of the ventilation rod 24, ensuring uniform airflow. The ventilation rod 24 extends from one end of the third straight section 213 of the air guide bracket 21 towards the central axis of the tank body 1. The line connecting the projections of the four ventilation rods 24 extending towards the central axis of the tank body 1 is approximately a square. The optimal effect is achieved when the ventilation rods 24 extend to or beyond the central axis, allowing all four sets of ventilation rods 24 to simultaneously deliver sterile air into the tank.

[0120] like Figure 9As shown, in this embodiment, the bottom sidewall 11 of an adventitious root culture tank is provided with at least four sets of air intake structures 12. The four sets of air intake structures 12 are evenly arranged inside the bottom sidewall 11 and are spaced at equal intervals. The air vents 24 of the air intake structures 12 are kept horizontal and their length is greater than the diameter of the base plate 23. The projections of any two opposing sets of air vents 24 inside the tank 1 are approximately parallel, and the projections of two adjacent sets of air vents 24 inside the tank 1 are approximately perpendicular. When the air intake structures 12 introduce sterile air into the tank 1, the gas flow force plays a better role in stirring the adventitious roots and culture medium inside the tank 1, and can provide sufficient sterile air to the tank 1 for the adventitious roots to breathe. A good supply of sterile air can promote the respiratory metabolism and root growth of adventitious roots.

[0121] The venting rod 24 is a long column with a hollow interior for air intake and the cavity wall is covered with micropores for air exhaust. One end of the venting rod 24 is spaced from the bottom wall, and the other end is tangent to or intersects the center line of the tank 1, so that the projection of the extension direction of the venting rod 24 inside the tank 1 is approximately parallel.

[0122] like Figure 1 As shown, the air intake structure 12 is connected in pairs to an air intake manifold A1106. Taking one air intake manifold A1106 as an example, the extension distance of the air intake manifold A1106 is less than the maximum radius of the culture tank. After extending a certain distance, the air intake manifold A1106 bends towards the front of the main support frame and extends through the main support frame to the same plane as the central axis of the culture tank. This plane is parallel to the front of the main support frame, so that the two air intake manifolds A1106 are symmetrically located below the culture tank and close to the center of the bottom of the culture device. After extending a certain distance, the air intake manifold A1106 extends vertically upwards for a certain distance and then extends two air intake branch pipes A1107 to both sides through the air intake tee pipe B8. The two air intake branch pipes A1107 The distance between the pipe and the inlet structure 12 is greater than the distance between the central axis of the culture tank and the inlet structure 12. The pipe bends upward at an angle and extends to connect with the two inlet structures 12 on the same side of the bottom of the culture tank. Another inlet branch pipe A1106 is symmetrically distributed with it. The two inlet branch pipes A1107 on the symmetrical side are connected to the two inlet structures 12 on the same side. The angle between the upward bend of the pipe and the pipe extending through the tee pipe is less than 90°. The pipe is connected to the four inlet structures 12 at the bottom of the culture tank through the inlet tee pipe B8. Air is evenly delivered to the culture tank through the inlet branch pipe A1107 extending from the inlet tee pipe B8, which greatly improves the contact surface between air and culture medium. The pipes of the extended frame are hidden under the tank body, making full use of space without affecting the arrangement of other pipes.

[0123] like Figure 9As shown, in one embodiment of the present invention, at least four sets of air inlet structures 12 and at least two sets of cutting structures 20 are provided on the bottom sidewall 11 of an adventitious root culture tank. They are located at different heights inside the tank bottom 11 near the discharge port 3 and around the discharge port 3. A cutting structure 20 is provided between every two sets of air inlet structures 12. The line connecting the installation position of a set of cutting structures 20 on the same side of the tank bottom 11 and the installation positions of two adjacent sets of air inlet structures 12 inside the tank body 1 approximately forms an isosceles triangle. The installation position of the cutting structure 20 is higher than the air inlet structure 12 in the vertical direction.

[0124] The relative positions of the air intake structure 12 and the cutting structure 20 cause the culture medium and the flow of adventitious roots in the tank 1 to form a vortex. This vortex is generated by the combined action of the cutting structure 20 cutting the adventitious roots and the upward movement of the bubbles generated when sterile air is introduced by the air intake structure 12.

[0125] The air intake structure 12 provides upward-flowing gas into the tank 1 and generates downward turbulence in the culture medium inside the tank 1. The turbulence causes the disordered plant tissue to align in the same direction and transports the plant tissue to the cutting structure 20, where it enters the cut of the cutting device and is cut into small segments.

[0126] The introduction of sterile air into the tank 1 reduces the liquid density at the bottom of the tank 1. Air bubbles generated by the venting rod 24 disperse the adventitious roots at the bottom of the tank 1, preventing them from tangling or becoming intertwined. The air intake structure 12 provides an upward buoyancy when supplying sterile air into the tank 1. This buoyancy causes the liquid at the bottom of the tank 1 to flow, raising the adventitious roots towards the middle of the tank 1. When the adventitious roots reach a certain height, they naturally flow back to the bottom of the tank 1 due to gravity. This process causes the adventitious roots to tumble and churn within the tank 1. During this tumbling process, the adventitious roots are attracted by the attractive force generated by the rotation of the shearing section 202 of the cutting structure 20.

[0127] When the shearing part 202 inside the cutting structure 20 rotates, it will cause the blade assembly 325 inside the shearing part 202 to rotate rapidly. The fluid flow force generated by the rotation of the blade assembly 325 will cause a low-pressure area to be formed around the cutting structure 20, and a high negative pressure to be generated around the shearing part 202. When the adventitious root approaches the shearing part 202, it will be attracted by the shearing part 202 and sheared.

[0128] The installation method of the air intake structure 12 and the cutting structure 20 can better trim and maintain the adventitious roots, provide sufficient sterile air for the adventitious roots, and better control the length of the adventitious roots, avoiding the adventitious root system from being too long and dense, which is not conducive to material discharge and processing.

[0129] The air intake structure 12 and the cutting mechanism 6 are respectively arranged symmetrically with respect to the center of the tank body 1.

[0130] One end of the vent rod 24 of the air intake structure 12 is spaced from the bottom of the tank 11, and the other end is tangent to or intersects the center line of the tank body 1, so that the projection of the extension direction of the vent rod 24 inside the tank body 1 is approximately parallel.

[0131] The gas provided by the aeration rod 24 causes the culture medium and plant tissue in the tank 1 to circulate along a parabolic trajectory that rises near the central axis and falls near the inner wall of the tank.

[0132] The cutting mechanism 6 has a cut end that extends toward the center line of the tank and has a gap between the ends to allow the culture medium and plant tissue rising near the central axis to pass through.

[0133] As the culture medium rises and falls along a near-parabolic trajectory, it exerts a driving force on the plant tissue, causing it to align regularly in the same direction and flow downwards into the cut of the cutting structure 20.

[0134] The cutting structure 20 and the air intake structure 12 are configured in this way to provide uniformly distributed gas into the tank 1. Since the distribution ratio of the venting rod 24 at the center line of the tank 1 is higher than that at the bottom 11 of the tank, the kinetic energy provided by the gas in the center line area is greater than the potential energy and the pressure above the culture medium. The culture medium can move upward under the influence of the airflow. When it reaches a certain height, it is far away from the venting rod 24, and the kinetic energy generated by the gas on the culture medium weakens and becomes less than the potential energy and the pressure above the culture medium. At the same time, the potential energy and the pressure above the culture medium at the inner wall are greater than the kinetic energy provided by the gas. This allows the culture medium to rise along the area near the central axis, then diffuse to the periphery, and circulate along the trajectory of the outer parabolic surface that descends near the inner wall of the tank. The moving culture medium guides the plant tissue to arrange in a regular manner and flow into the cut of the cutting mechanism 6. At the same time, the rotation of the cutter in the cutting mechanism 6 generates vortex suction, which further draws the regularly arranged plant tissue into the cut of the cutting mechanism 6, ensuring the uniformity of the cut and preventing uneven cutting of entangled plant tissue or repeated cutting of plant tissue.

[0135] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An adventitious root culture tank, characterized by, The application relates to a kind of indefinite root culture tanks, comprising: A tank body, a tank bottom, and a discharge port provided on the tank bottom; the middle part of the tank body is in the shape of a cylinder, and the tank bottom is gradually tapered from the bottom end of the middle part of the tank body to the discharge port to form the shape of an inverted cone; At least four groups of air inlet structures and at least two groups of cutting structures are provided on the side wall of the tank bottom and are arranged at different heights near the discharge port and around the discharge port in the tank bottom; one group of cutting structures is arranged between every two groups of air inlet structures; the installation positions of one group of cutting structures and the installation positions of the adjacent two groups of air inlet structures on the same side of the tank bottom form an isosceles triangle in the tank body; the installation position of the cutting structure is higher than that of the air inlet structure in the vertical direction; The air inlet structure is provided with an air inlet rod in the culture tank; the rod body of the air inlet rod is hollow; and a large number of micropores are densely arranged on the surface of the air inlet rod; The positions of the four groups of air inlet rods extending towards the central axis of the tank body are approximately square in projection in the tank body; The cutting structure is provided with a transmission part between the shearing part and the power part; and the power part can drive the shearing part to cut the indefinite roots; The blade groups of the cutting structure are arranged at intervals on the central axis of the shearing part; each blade group comprises a first blade and a second blade; all the first blades in the blade group are parallel to each other and are fixed to the rotating shaft; and the second blade rotates relative to the first blade to form a continuously opening and closing cutout; An exhaust device is installed on the tank cover and is used for exhausting waste gas in the tank body; A feeding device is arranged above the outer side wall of the tank body; An upper expansion type tank bottom valve is arranged at the bottom of the tank body and is embedded in the discharge port; The height between the tank cover and the discharge port is defined as H; the diameter of the tank body is defined as D; and the ratio between the height H and the diameter D of the tank body is between 1:0.5 and 1:1.

2.

2. The indefinite root culture tank according to claim 1, wherein The cutting structure is provided with a spacer sleeve; a circular mounting hole is arranged on the tank bottom; the outer circumferential surface of the spacer sleeve is in the shape of a cylinder and has the same diameter as the mounting hole; and the spacer sleeve is detachably arranged in the mounting hole on the tank bottom; One side of the spacer sleeve facing the inside of the tank body is connected to the shearing part; and the other side of the spacer sleeve facing the outside of the indefinite root culture tank is connected to the power part; The cross section of the shearing part is smaller than that of the spacer sleeve.

3. The rhizome culture tank according to claim 1, wherein The blade groups are arranged perpendicularly to the central axis of the cutting structure; The shearing part comprises at least two groups of blade groups connected in series on the rotating shaft; the blade groups are arranged at intervals along the axis direction of the rotating shaft to form a multi-layer structure; and each blade group is composed of two first blades and two second blades.

4. The rhizome culture tank according to claim 1, wherein The top of the tank body is provided with the part of the tank cover in the shape of a circular arc.

5. The indefinite root culture tank according to claim 1, wherein The air inlet structure is connected to an air inlet branch pipe and an air inlet three-way pipe; the air inlet three-way pipe is connected to an air inlet branch pipe and supplies air to the air inlet structure.

6. The indefinite root culture tank according to claim 5, wherein The air inlet branch pipe is formed by extending the air inlet branch pipe in the direction of the central axis of the tank body for a length and then extending the air inlet branch pipe in a direction perpendicular to the air inlet branch pipe for a length; and the lengths of the air inlet branch pipes at the two ends of the air inlet branch pipe are equal. The air intake branch is connected with an air intake part of the air intake structure, and the air intake branch and the air intake branch provide the air intake structure with air uniformly and provide the air with the same pressure.

7. The adventitious root culture tank according to claim 1, characterized in that, An interface is formed on the bottom of the tank; the air intake structure comprises an air intake part, an air rod and a gas guide support connecting the air intake part and the air rod, and further comprises a seat plate which can be embedded at the interface; the seat plate is sleeved on the outer periphery of the gas guide support and connected with the edge of the interface by the edge thereof, so that the air rod is separated from the air intake part inside and outside the adventitious root culture tank; The gas guide support is a bent pipeline comprising a first straight section, a second straight section and a third straight section; the first straight section is arranged perpendicularly to the second straight section, and the second straight section and the third straight section are arranged at an obtuse angle; the first straight section and the second straight section and the second straight section and the third straight section are smoothly connected.

8. The adventitious root culture tank according to claim 7, characterized in that, The air intake structure comprises the air rod which is kept horizontal and has a certain length, and the air rod is in the shape of a long column.

9. The rhizome culture tank according to claim 1, wherein The upwardly-expanding tank bottom valve comprises a valve core, a valve rod, The valve core is matched with the shape of the discharge port; The valve rod is connected with the valve core and used for driving the valve core to move along the axial direction of the discharge port.

10. The rhizome culture tank according to claim 1, wherein The exhaust device comprises: An air inlet for connecting the exhaust device with the tank body; An exhaust port for connecting the exhaust device with a waste gas exhaust pipe; The exhaust device further comprises cooling water inlets and outlets arranged on both sides, for condensing and liquefying water vapor in the exhaust device.

11. The rhizome culture tank according to claim 1, wherein The feeding device comprises feeding structure, acid supplement structure, alkali supplement structure and defoaming liquid supplement structure arranged transversely in sequence; The feeding structure comprises a feeding pneumatic valve for controlling the opening and closing of a feeding pipeline, and supplementing the culture solution required in the tank body; The acid supplement structure comprises an acid supplement pneumatic valve for controlling the opening and closing of an acid supplement pipeline, and supplementing the acidic solution required in the tank body; The alkali supplement structure comprises an alkali supplement pneumatic valve for controlling the opening and closing of an alkali supplement pipeline, and supplementing the alkaline solution required in the tank body; The defoaming liquid supplement structure comprises a defoaming liquid supplement pneumatic valve for controlling the opening and closing of a defoaming liquid supplement pipeline, and supplementing the defoaming liquid in the tank body.

12. The adventitious root culture tank according to any one of claims 1 to 11, wherein The feeding device further comprises a feeding tank, an acid supplement tank, an alkali supplement tank and a defoaming liquid supplement tank, which are arranged vertically in sequence; When the feeding tank detects that the culture solution needs to be supplemented in the tank body, the feeding pneumatic valve is opened, and the culture solution is transported to the tank body through a hose; The acid supplement tank, the alkali supplement tank and the defoaming liquid supplement tank have the same feeding mode as the feeding pneumatic valve.

13. A rhizotron tank according to claim 12, wherein One end of the feeding structure is connected with a feeding air inlet pipe, and the other end is connected with a feeding air outlet pipe; a feeding air inlet valve is arranged on the feeding air inlet pipe, and a feeding exhaust valve is arranged on the feeding air outlet pipe; before feeding the tank body, the inside of the feeding structure needs to be sterilized through a high-temperature steam pipeline; The acid supplement structure, the alkali supplement structure and the defoaming liquid supplement structure have the same connection mode with the pipelines.

14. The rhizome culture tank according to claim 13, wherein The feeding air inlet pipe of the feeding pneumatic valve is connected with a high-temperature steam air inlet pipe, and the feeding air outlet pipe is connected with a high-temperature steam exhaust pipe. The acid supplement pneumatic valve, the alkali supplement pneumatic valve and the defoaming liquid supplement pneumatic valve have the same connection mode between the air inlet pipe and the high-temperature steam air inlet pipe.

15. A rhizotron tank according to claim 14, wherein One end of the exhaust air pipe is connected with the exhaust port of the exhaust device, and the other end is connected with the tail gas filter.

16. A rhizotron according to claim 15, wherein, The high-temperature steam air inlet pipe is connected with a high-temperature steam main pipe, steam in the culture tank enters the high-temperature steam air inlet pipe from the high-temperature steam main pipe to the air inlet pipes of the respective pneumatic valves of the feeding device, and the waste gas generated after sterilization is discharged through the high-temperature steam exhaust pipe.

Images