A bactericidal composition for improving the prevention and treatment effect of jackfruit fruit rot

Abstract

The present invention relates to the field of pesticide technology, and more specifically to a fungicide composition that improves the effectiveness of preventing and treating jackfruit fruit rot. The fungicide composition, comprising a compound of farnesol and cyproconazole as active ingredients; the mass ratio of farnesol to cyproconazole is 1-9:5-1. Based on existing pesticides, the present invention compounds different active ingredients of pesticides to screen for synergistic combinations. This combination can improve the effectiveness of preventing and treating jackfruit fruit rot, reduce pesticide application dosage, and delay the development of drug resistance in pathogens.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a bactericidal composition capable of improving the prevention and treatment effect of jackfruit fruit rot. Background Art

[0002] Both Cylindrocladium sp. and L. theobromae can cause jackfruit fruit rot. L. theobromae can parasitize over 500 plant species in tropical and subtropical regions, causing a variety of diseases in field crops, vegetables, fruits, and forest trees, including dieback, branch dieback, root rot, fruit rot, leaf spot, ulcers, and gummosis.

[0003] Jackfruit dactylic acid (Dipora caespitosa) fruit rot primarily affects the male flowers and fruit. When the male flowers are infected, the initial infected area turns brown and quickly spreads to the surrounding area, quickly infecting the entire flower. In later stages, the entire flower turns black and falls off. The disease can occur anywhere on the fruit, with irregular lesions. Initially, the infected peel turns brown with white mycelium. In later stages, the initial infected area turns dark brown, causing fruit rot and impacting both yield and quality.

[0004] Currently, chemical pesticides are one of the most effective means of controlling jackfruit fruit rot. However, the long-term, unscientific use of single, continuous, and repetitive pesticides has caused the fruit rot pathogen to develop varying degrees of resistance to various currently used pesticides. Furthermore, this unscientific use shortens the time it takes for resistance to develop, significantly increasing control costs, environmental costs, and pesticide research and development costs. Given this current situation, improving the effectiveness of chemical control is a cost-effective and effective way to address these issues.

[0005] Combining the active ingredients of different pesticides is an effective and efficient method for developing new pesticides and combating resistant agricultural pathogens. The active ingredients of different pesticides typically exhibit three types of interactions: additive, synergistic, and antagonistic. Formulas with excellent synergistic effects can significantly improve control effectiveness, reduce pesticide usage, and significantly slow the development of resistance in pathogens, making them an important tool for comprehensive disease control.

[0006] Through indoor biological activity tests, the inventors found that when farnesol is compounded with fluazifop, benzovintriazole or cyproconazole within a certain mass ratio range, it exhibits a synergistic effect on jackfruit fruit rot. Currently, there are no reports on such compounding. Summary of the Invention

[0007] The object of the present invention is to provide a fungicide composition for improving the prevention and control effect of jackfruit fruit rot. On the basis of existing pesticides, different pesticide active ingredients are compounded to screen out a combination with synergistic effect, which can improve the prevention and control effect of jackfruit fruit rot, help reduce the pesticide application dosage, and delay the development of pathogen resistance.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A fungicide composition for improving the prevention and control effect of jackfruit fruit rot disease. The effective component of the fungicide composition is prepared by compounding farnesol with fluazifop, benzovintriazole or cyproconazole.

[0010] Preferably, the mass ratio of farnesol to pentozolin is 1-40:2-1.

[0011] Preferably, the mass ratio of farnesol to benzovindiflupyr is 1-10:25-1.

[0012] Preferably, the mass ratio of farnesol to cyproconazole is 1-9:5-1.

[0013] Another object of the present invention is to provide an application of the fungicide composition in preventing and treating jackfruit fruit rot.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The effective ingredient farnesol in the composition of the present invention has a good synergistic effect after being compounded with fluazifop, benzovintriazole or cyproconazole, can improve the prevention and control effect of jackfruit fruit rot, is conducive to reducing the dosage of pesticide application, and delays the development of pathogen resistance. DETAILED DESCRIPTION

[0016] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0017] Example :Indoor bioactivity test of farnesol complex against jackfruit fruit rot

[0018] Experimental subjects: Diseased fruits were selected from jackfruit plantations, and Lasiodiplodia theobromae was isolated and purified in the laboratory and stored on PDA culture medium.

[0019] Test agents: 98% farnesol technical (Shanghai Yuanye Biotechnology Co., Ltd.), 96% fluazifop technical (Syngenta Nantong Crop Protection Co., Ltd.), 96% benzotriazole technical (Syngenta Nantong Crop Protection Co., Ltd.), 95% cyproconazole technical (Xiangshui Zhongshan Biotechnology Co., Ltd.)

[0020] Test method: (Refer to "NY / T 1156.2-2006 Pesticide Indoor Bioassay Test Guidelines Fungicides Part 2: Inhibition of Pathogenic Fungal Mycelial Growth Test Plate Method")

[0021] 1. Dissolve the original drug in dimethyl sulfoxide first, then dilute with 0.1% Tween-80 aqueous solution to prepare single-dose stock solutions. Set up multiple groups of proportions, and set 5 gradient mass concentrations for each single dose and each group of mixtures.

[0022] 2. Add 9 mL of pre-thawed PDA medium to a sterile conical flask. Quantitatively pipette 1 mL of the drug solution from the low and high concentrations into each of the flasks and shake thoroughly. Then, pour equal amounts into three 9 cm diameter culture dishes to create drug-containing plates of the corresponding concentrations. A blank control should be prepared without drug. Each treatment should be replicated three times.

[0023] 3. Use a hole puncher to take a 5mm diameter bacterial cake from the expanded Colletotrichum gloeosporioides colony, inoculate the center of the drug-containing plate and the blank control plate, and culture in a constant temperature incubator at 28℃.

[0024] 4. After 24 hours, the colony diameter was measured using the cross-cross method, and the mycelial growth inhibition rate of different treatments was calculated. The logarithm value of the agent was used as the independent variable, and the probability value of mycelial growth inhibition rate was used as the dependent variable. The DPS software was used for analysis to obtain the toxicity regression equation and the toxicity EC value of the agent to the target pathogen. 50 The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0025] D = D1 - D2, where D is the diameter of the colony growth; D1 is the diameter of the colony; D2 is the diameter of the cake;

[0026] I=[(D0-D t )÷D0]×100, where I is the mycelial growth inhibition rate (%); D0 is the colony growth diameter of the blank control; D t The diameter of the colony growth after drug treatment;

[0027] Measured toxicity index (ATI) = (standard agent EC 50 ÷Test agent EC 50 )×100;

[0028] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture;

[0029] Co-toxicity coefficient (CTC) = [measured toxicity index (ATI) of the mixture ÷ theoretical toxicity index (TTI) of the mixture] × 100.

[0030] The criteria for categorizing combined effects are: a CTC ≥ 120 indicates synergism; a CTC ≤ 80 indicates antagonism; and a CTC 80 < 120 indicates additive effects. The experimental results are shown in Tables 1-3.

[0031] Table 1 Indoor bioactivity determination of farnesol and pentozolin against jackfruit fruit rot pathogens

[0032] Drug name and ratio EC50(mg / L) ATI TTI CTC Farnesol 18.3491 100.0000 -- -- cypermethrin 1.7283 1061.6849 -- -- Farnesol 1: oxazolidinone 2 1.8704 981.0254 741.1233 132.3701 Farnesol 1: oxazolidinone 1 2.5511 719.2623 580.8424 123.8309 Farnesol 3: oxazolidinone 1 3.7088 494.7449 340.4212 145.3332 Farnesol 7: oxazolidinone 1 6.0529 303.1456 220.2106 137.6617 Farnesol 15: Fluoxetine 1 4.4881 408.8389 160.1053 255.3563 Farnesol 30: cypermethrin 1 9.8974 185.3931 131.0221 141.4976 Farnesol 40: cyfluthrin 1 11.5630 158.6881 123.4557 128.5384

[0033] As shown in Table 1, within the mass ratio of 1-40:2-1, the co-toxicity coefficient of farnesol and cyfluthrin against jackfruit fruit rot pathogens ranged from 123.8309 to 255.3563, all greater than 120, showing a synergistic effect.

[0034] Table 2 Indoor bioactivity determination of farnesol and benzovinflumizone complex against jackfruit fruit rot pathogens

[0035] Drug name and ratio EC50(mg / L) ATI TTI CTC Farnesol 18.3491 100.0000 -- -- Benzovindiflupyr 10.5042 174.6835 -- -- Farnesol 1: Benzovinflumazole 25 8.8170 208.1105 171.8110 121.1275 Farnesol 1: Benzovinflumazole 15 5.0460 363.6365 170.0157 213.8840 Farnesol 1: Benzovinflumazole 7 7.1186 257.7628 165.3480 155.8910 Farnesol 1: Benzovinflumazole 3 2.8687 639.6312 156.0126 409.9869 Farnesol 1: Benzovinflumazole 1 4.0190 456.5588 137.3417 332.4254 Farnesol 3: Benzovinflumazole 1 3.4194 536.6175 118.6709 452.1898 Farnesol 7: Benzovinflumazole 1 1.4122 1299.3273 109.3354 1188.3863 Farnesol 10: Benzovinflumazole 1 10.0151 183.2143 106.7894 171.5660

[0036] As shown in Table 2, within the mass ratio of 1-10:25-1, the co-toxicity coefficient of farnesol and benzovinfluzide against jackfruit fruit rot pathogens was between 121.1275-1188.3863, all greater than 120, showing a synergistic effect; especially when the mass ratio was 7:1, the co-toxicity coefficient reached 1188.3863, and the synergistic effect was most significant.

[0037] Table 3 Indoor bioactivity determination of farnesol and cyproconazole complex against jackfruit fruit rot pathogens

[0038] Drug name and ratio EC50(mg / L) ATI TTI CTC Farnesol 18.3491 100.0000 -- -- Cyproconazole 3.1429 583.8270 -- -- Farnesol 1: Cyproconazole 5 2.6527 691.7141 503.1892 137.4660 Farnesol 1: Cyproconazole 3 3.1786 577.2699 462.8703 124.7153 Farnesol 1: Cyproconazole 1 4.0142 457.1048 341.9135 133.6902 Farnesol 3: Cyproconazole 1 5.0338 364.5179 220.9568 164.9725 Farnesol 5: Cyproconazole 1 7.7554 236.5977 180.6378 130.9790 Farnesol 9: Cyproconazole 1 10.0853 181.9391 148.3827 122.6147

[0039] As shown in Table 3, within the mass ratio of 1-9:5-1, the co-toxicity coefficient of farnesol and cyproconazole against jackfruit fruit rot pathogens ranged from 122.6147 to 164.9725, all greater than 120, showing a synergistic effect.

[0040] In summary, the active ingredient farnesol in the composition of the present invention has a good synergistic effect when combined with fluazifop, benzovintriazole or cyproconazole, which can improve the prevention and control effect of jackfruit fruit rot, help reduce the dosage of pesticide application, and delay the development of pathogen resistance.

[0041] The above is only a preferred embodiment of the present invention. Those skilled in the art can make appropriate improvements without departing from the principles of the present invention. These improvements are also within the scope of protection of the present invention.

Claims

1. A bactericidal composition for improving the prevention and control effect of jackfruit fruit rot, characterized in that: The active ingredient of the bactericidal composition is prepared by compounding farnesol and cyproconazole; the mass ratio of the farnesol to cyproconazole is 1-9:5-1.

2. The use of the bactericidal composition according to claim 1 in preventing and treating jackfruit fruit rot, characterized in that: The pathogen of the jackfruit fruit rot is Diplodia theobromin.